This study aimed to assess effect of nitrogen (N) form and phosphorus (P) level on the growth and mineral composition of hybrid Napier grass. Experimental plants were grown with different N forms (NO3−, NH4NO3, and NH4+; 500 µM) and P concentrations (100 and 500 µM) under greenhouse conditions for 42 days. Growth rate, morphology, pigments, and mineral nutrients in the plant tissue were analysed. At the low P concentration, the better growth was found in the plants supplied with NH4+ (relative growth rate (RGR) = 0.05 g·g−1·d−1), but at the high P concentration, the NH4+-fed plants had 37% lower growth rates and shorter roots and stems. At the high P level, the NH4NO3−-fed plants had the highest RGR (0.04 g·g−1·d−1). The mineral nutrient concentrations in the plant tissues were only slightly affected by N form and P concentration, although the P concentrations in the plant tissue of the NO3−-fed plants supplied with the high P concentration was 26% higher compared to the low P concentration plants. The N concentrations in the plant tissues did not vary between treatments. The results showed that the optimum N form for the plant growth and biomass productivity of hybrid Napier grass depends on P level. Hybrid Napier grass may be irrigated by treated wastewater containing high concentrations of N and P, but future studies are needed to evaluate biomass production and composition when irrigating with real wastewater from animal farms.
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