High Production Research Articles

Engineering the Non-catalytic Domain to Enhance Catalytic Activity and Thermal Stability of a Nκ2-Producing κ-Carrageenase.

κ-Carrageenases play an important role in achieving the high-value utilization of carrageenan polysaccharides. They can be used in the preparation of even-numbered κ-neocarrageenan oligosaccharides by degrading κ-carrageenan (KC). We previously identified and characterized a κ-carrageenase, CaKC16B, with high specificity for producing a single κ-neocarrabiose. It can produce a single κ-neocarrabiose from degrading KC. However, they also exhibited poor thermal stability and catalytic efficiency. To improve these properties, we introduced noncatalytic domains (nonCDs) from a heat-resistant κ-carrageenase, MtKC16A, into the C-terminus of CaKC16B to construct CaKC16BUN and CaKC16BUNB. Compared to the original CaKC16B, both of them exhibited improved enzymatic properties, including optimal reaction temperature, thermal stability, substrate affinity, and catalytic efficiency. Remarkably, the kcat/Km values of 16BUN and 16BUNB increased by 127 and 290 times, respectively. Importantly, the addition of nonCDs did not alter the final products of degrading KC, retaining the high product specificity of CaKC16B. Interestingly, the addition of nonCDs changed CaKC16B's substrate specificity for hydrolyzing KC and βκ-carrageenan, with mutants exhibiting higher relative activity for βκ-carrageenan. We further observed through biolayer interferometry that the binding and dissociation process between MtUN nonCD and βκ-carrageenan is faster compared to that of KC. This may explain the change in the substrate specificity observed in the mutants of CaKC16B.

Co Cluster-Modified Ni Nanoparticles with Superior Light-Driven Thermocatalytic CO2 Reduction by CH4

Excessive fossil burning causes energy shortages and contributes to the environmental crisis. Light-driven thermocatalytic CO2 reduction by methane (CRM) provides an effective strategy to conquer these two global challenges. Ni-based catalysts have been developed as candidates for CRM that are comparable to the noble metal catalysts. However, they are prone to deactivation due to the thermodynamically inevitable coking side reactions. Herein, we reported a novel Co-Ni/SiO2 nanocomposite of Co cluster-modified Ni nanoparticles, which greatly enhance the catalytic durability for light-driven thermocatalytic CRM. It exhibits high production rates of H2 (rH2) and CO (rCO, 22.8 and 26.7 mmol min−1 g−1, respectively), and very high light-to-fuel efficiency (ƞ) is achieved (26.8%). Co-Ni/SiO2 shows better catalytic durability than the referenced catalyst of Ni/SiO2. Based on the experimental results of TG-MS, TEM, and HRTEM, we revealed the origin of the significantly enhanced light-driven thermocatalytic activity and durability as well as the novel photoactivation. It was discovered that the focused irradiation markedly reduces the apparent activation energy of CO2 on the Co-Ni/SiO2 nanocomposite, thus significantly enhancing the light-driven thermocatalytic activity.

“Antimicrobial and antioxidant capacity of Dunaliella salina, Tetraselmis chuii and Isochrysis galbana and their potential use in food.” a systematic review

AbstractThe main compounds extracted from the biomass of marine microalgae have antioxidant, antimicrobial, antifungal, anti-inflammatory and antitumor effects, making the possibility of using these properties in the development of foods feasible. Despite the proven biological activity of microalgae, there are still challenges regarding the production and use of microalgal biomass or its derivatives in food industries that are related to high production costs, and there is little research regarding the evaluation of their safety and the search for their application in food development. Therefore, this research aimed to collect information regarding the biological activities of marine microalgae, which allows their use as a natural antimicrobial additive in food matrices and as an ingredient in the development of functional foods with antioxidant capacity. The search interval for the PRISMA (preferred reporting items for systematic reviews and meta-analyses) guidelines was five years. Different methods for the extraction of antioxidant and antimicrobial compounds from the marine microalgae Dunaliella salina, Tetraselmis chuii and Isochrysis galbana were compared and discussed, and the viability of their use and application in food matrices and in the food industry in general were analyzed. It was concluded that there are research gaps in the use of microalgae biomass as an extract, the identification of bioactive molecules for use in the food industry as antimicrobial agents and for the development of functional foods with antioxidant capacity.

Enhanced vitamin B12 production by isolated Bacillus strains with the application of response surface methodology.

Vitamin B12 is a crucial B-group vitamin, first isolated from the liver due to its role in combating pernicious anemia. It is distinguished by its unique and complex structure, which makes its chemical synthesis challenging and expensive. Consequently, vitamin B12 is alternatively obtained through microbial fermentations. Molasses, an affordable and safe agro-industrial waste, can be used as a carbon source for vitamin B12 production, offering a cost-effective alternative to expensive sugars in the production medium. A total of 87 yeast, actinomycete, and bacterial isolates were screened for vitamin B12 production, with 15 isolates showing high productivity. Bacillus isolates were selected for further analysis using MALDI-TOF and molecular identification. These isolates were identified as four strains of Bacillus subtilis (MZ08, JT10, BY11, and JT17), one strains of Bacillus sp. (CB09), and one strain of Peribacillus acanthi (MZ01). Genetic circuits associated with vitamin B12 production were demonstrated in a closely related strain of Peribacillus acanthi MZ01 strain. Three strains (MZ01, MZ08, and JT17) were selected for further evaluation of vitamin B12 productivity under different sugar types (glucose, sucrose, fructose, lactose, and galactose) and varying inoculum sizes. The inoculum size significantly impacted vitamin B12 production, with an increase from 5 to 10% enhancing yields. The ability of the strains to produce vitamin B12 varied depending on the type of sugar used. Peribacillus acanthi MZ01 strain showed the highest productivity and subsequently, selected for optimizing vitamin B12 production conditions using response surface methodology. Furthermore, the optimized conditions were then applied to molasses-based medium to achieve high vitamin B12 yields by MZ01 strain. In this study, Peribacillus acanthi was characterized for the first time as a vitamin B12 producer, demonstrating high productivity among various tested strains. The optimization of production conditions using response surface methodology, further enhanced vitamin B12 yields, showcasing the strain's efficiency in microbial fermentations. This research also highlights the potential of using molasses as a cost-effective alternative carbon source, significantly reducing production costs.

Influential Factors of Indonesian Cocoa Export: Evidence from FMOLS and DOLS Approaches

The study investigated the determinants of cocoa exports using both FMOLS and DOLS approaches. The time series data were obtained from FAOSTAT and ICCO websites from the year 1990 to 2022. ADF and PP stationary unit roots were conducted to examine the stationarity of the variables while Johansen cointegration was used to verify the cointegrating among the variables. The outcome of the Johansen cointegration analysis suggests the existence of a cointegration relationship among the eight variables, indicating the presence of long-run relationships among the variables. The estimated results revealed that cocoa productivity, domestic cocoa production, domestic cocoa supply, exchange rates, and world cocoa prices affect Indonesian cocoa exports. Based on the estimated results and discussion, the study draws a conclusion that cocoa productivity, domestic cocoa production, exchange rates, and world cocoa prices positively and statistically significantly influenced cocoa exports while domestic cocoa supply negatively affected Indonesian cocoa exports. The study recommends that the government of Indonesia should stabilize the fluctuating exchange rates and encourage cocoa production via higher productivity by practicing farm rehabilitation practices to increase productivity and cocoa beans for exports. Moreover, the supply of cocoa beans should also be increased by increasing domestic producer prices. These key determinants are crucial for policymakers and industry stakeholders to understand and address to enhance the competitiveness and growth of the cocoa export industry in Indonesia.

Impact of Exopolysaccharides from Fructilactobacillus sanfranciscensis Ls5 on the Quality of Mantou: A Comparative Study of Fermentation Conditions

Lactic acid bacteria (LAB) and their exopolysaccharides (EPS) have the potential to enhance the quality of flour-based products. This study investigated the effect of EPS produced by Fructilactobacillus sanfranciscensis Ls5 on the quality of Mantou. LAB strains with high EPS production were isolated from traditional fermenters, and their characteristics and EPS properties were examined. Four different fermentation conditions (Blank, Yeast, Ls5-Yeast, and EPS-Yeast) were compared in terms of their effects on the physicochemical properties, in vitro digestion characteristics, and shelf-life of Mantou. The incorporation of Ls5 and its EPS into Mantou resulted in a lower dough fermentation pH, increased organic acid production, and enhanced fermentation activity. Additionally, the incorporation of Ls5 and its EPS led to significant improvements in the quality of the Mantou, including the extension of shelf-life, improved sensory evaluation, and a reduction in the sugar content. Additionally, there was an increase in resistant starch content during digestion in both types of Mantou, which could offer potential benefits to human glycemic health.

GESTÃO SUSTENTÁVEL DE RESÍDUOS NA INDÚSTRIA DE LATICÍNIOS: ESTRATÉGIAS PARA O REAPROVEITAMENTO DO SORO DO LEITE

Whey, one of the main by-products generated during the production of cheese and other dairy products, has a rich composition that can be transformed into relevant resources. In this context, various technologies, such as membrane filtration, microfiltration, ultrafiltration, nanofiltration and reverse osmosis, are suitable for separating and concentrating important components, such as proteins and lactose, from whey. In addition, spray-drying transforms whey into high value-added products, while anaerobic digestion allows waste to be converted into biogas, promoting the generation of renewable energy. Reviewing the approach to integrating these technologies not only reduces the environmental impact of the dairy industry, but also contributes to economic efficiency, promoting a circular economy. The article concludes that adopting these practices is essential to ensure a sustainable future for the sector, benefiting both the industry and the environment.

Impact of Arable Land Abandonment on Crop Production Losses in Ukraine During the Armed Conflict

The outbreak of Russia-Ukraine conflict casted an impact on the global food market, which was believed to be attributed to that Ukraine has suffered significant production losses due to cropland abandonment. Nevertheless, recent outbreaks of farmer protests against Ukraine’s grain exports demonstrated that the production losses might not be as severe as previous estimates. By utilizing the adaptive threshold segmentation method to extract abandoned cropland from the Sentinel-2 high-resolution imagery and calibrating the spatial production allocation model’s gridded crop production data from Ukraine’s statistical data, this study explicitly evaluated Ukraine’s crop-specific production losses and the spatial heterogeneity. The results demonstrated that the estimated area of abandoned cropland in Ukraine ranges from 2.34 to 2.40 million hectares, constituting 7.14% to 7.30% of the total cropland. In Ukrainian-controlled zones, this area spans 1.44 to 1.48 million hectares, whereas in Russian-occupied areas, it varies from 0.90 to 0.92 million hectares. Additionally, the total production losses for wheat, maize, barley, and sunflower amount to 1.92, 1.67, 0.70, and 0.99 million tons, respectively, with corresponding loss ratios of 9.10%, 7.48%, 9.54%, and 8.67%. Furthermore, production losses of wheat, barley, and sunflower emerged in both the eastern and southern states adjacent to the conflict frontlines, while maize losses were concentrated in the western states. The findings imply that Ukraine ought to streamline the food transportation channels and maintain stable agricultural activities in regions with high crop production.

Distinct Pathways Determined by Photocatalytic C─H or O─H Bond Activation for Hydroxyl Compounds Conversion Paired With Hydrogen Production

AbstractSelective control of C─H or O─H bond activation in photocatalytic conversion coupled with hydrogen production is a promising yet challenging goal. Here, an efficient photocatalytic system is reported that can produce high‐valued tartaric acid or formaldehyde and simultaneous producing hydrogen. At optimized conditions, the directional conversion of glycolic acid into tartaric acid is achieved with a selectivity of 76.24%, and the selectivity of methanol oxidation into formaldehyde reaches 88.21%. A high hydrogen production rate of 21.43 mmol·g−1·h−1 is obtained using glycolic acid as substrate. Mechanism studies reveal that α‐C─H bond is preferentially activated in glycolic acid adsorbed on the photocatalyst, while O─H bond is preferentially activated in methanol, forming carbon‐centered radical (•CH(OH)COOH) or oxygen‐centered radical (CH3O•) for subsequent coupling or oxidation reactions. This work demonstrates the selective control of the photocatalytic conversion process of different hydroxyl compounds, providing a new perspective for achieving organic compounds selective activation coupled with hydrogen production.

Discovery of Lipoxygenase-Like Materials for Inducing Ferroptosis.

Recent research has highlighted the pivotal role of lipoxygenases in modulating ferroptosis and immune responses by catalyzing the generation of lipid peroxides. However, the limitations associated with protein enzymes, such as poor stability, low bioavailability, and high production costs, have motivated researchers to explore biomimetic materials with lipoxygenase-like activity. Here, we report the discovery of lipoxygenase-like two-dimensional (2D) MoS2nanosheets capable of catalyzing lipid peroxidation and inducing ferroptosis. The resulting catalytic products were successfully identified using mass spectrometry and a luminescent substrate. Unlike native lipoxygenases, MoS2 nanosheets exhibited exceptional catalytic activity at extreme pH, high temperature, high ionic strength, and organic solvent conditions. Structure-activity relationship analysis indicates that sulfur atomic vacancy sites on MoS2 nanosheets are responsible for their catalytic activity. Furthermore, the lipoxygenase-like activity of MoS2 nanosheets was demonstrated within mammalian cells and animal tissues, inducing distinctive ferroptotic cell death. In summary, this research introduces an alternative to lipoxygenase to regulate lipid peroxidation in cells, offering a promising avenue for ferroptosis induction.

From the player to the sponsor: exploring the interplay of attitudes in MMA sports sponsorship

PurposeThis study aims to investigate the complex dynamics of sports sponsorship within the context of mixed martial arts (MMA). Specifically, it explores how audience attitudes toward MMA influence their perceptions of players and sponsors, and subsequently, how these attitudes affect brand perceptions and purchase intentions. Furthermore, this research examines the moderating effect of fans' attitudes toward MMA on the relationship between their attitudes toward sponsors and their purchase intentions. MMA was selected as the focus of this study due to its propensity to evoke more intense emotional responses than other sports, as evidenced by previous research.Design/methodology/approachStudy 1 was conducted to systematically assess the emotional responses elicited by MMA. This preliminary study involved 277 participants. Building on the findings from Study 1, Study 2 employed an online survey to investigate the relationships among various constructs related to MMA. This survey was facilitated through an external portal and included a sample of 459 participants residing in the United States. The constructs measured in this survey were attitudes toward the player (ATP), attitudes toward the sponsor (ATS), perception of brand quality (PBQ), purchase intention (ITP) and attitudes toward mixed martial arts (ATM).FindingsThe study reveals that respondents rated MMA the most negatively among the analyzed sports, with the highest standard deviation, indicating that MMA evokes extreme emotional responses. The findings confirm that attitudes toward the player positively influence attitudes toward the sponsor, aligning with sponsorship-linked marketing theory. Moreover, positive attitudes toward the player were found to enhance perceptions of the sponsor’s brand quality. The research further demonstrated that attitudes toward the sponsor and perceptions of brand quality mediate the relationship between attitudes toward the player and purchase intentions, underscoring the complex nature of consumer decision-making in MMA sponsorship.Originality/valueThis research provides novel insights into the critical role of audience engagement and personal connection with sports in formulating effective sponsorship strategies. It emphasizes the need for marketers to tailor their approaches to align with the sport’s personal significance to the audience, employing narratives that resonate with their identities and values. Enhancing player-sponsor associations through co-branded campaigns and endorsements is essential. Furthermore, consistently maintaining high product quality and fostering emotional connections with the sport are key strategies to drive increased purchase intentions.

Hiring and firing – a signaling game

Abstract We study a signaling game between an employer and a potential employee, where the employee has private information regarding their production capacity. At the initial stage, the employee communicates a salary claim, after which the true production capacity is gradually revealed to the employer as the unknown drift of a Brownian motion representing the revenues generated by the employee. Subsequently, the employer has the possibility to choose a time to fire the employee in case the estimated production capacity falls short of the salary. In this setup, we use filtering and optimal stopping theory to derive an equilibrium in which the employee provides a randomized salary claim and the employer uses a threshold strategy in terms of the conditional probability for the high production capacity. The analysis is robust in the sense that various extensions of the basic model can be solved using the same methodology, including cases with positive firing costs, incomplete information about an individual’s own type, as well as an additional interview phase.

Facile Synthesis of Fluorescent Carbon Quantum Dots with High Product Yield Using a Solid-Phase Strategy

The liquid-phase method is the most commonly utilized strategy for synthesizing fluorescent carbon quantum dots (CQDs). However, the liquid-phase synthesis of CQDs faces challenges such as low yield, complex purification, and the use of toxic solvents, which limit large-scale production and practical applications. In this study, fluorescent CQDs with a high product yield of 78% were synthesized using glucose as a carbon source through a green and facile one-step solid-phase approach, without solvents or post-treatment. A systematic study of the structure and fluorescence properties of the synthesized CQDs was conducted using various characterization techniques. The results indicated that the mean size of obtained CQDs was 4.1 nm, and that their surface had abundant oxygen-containing functional groups, resulting in favorable water solubility. The synthesized CQDs exhibited excitation-dependent fluorescence, with optimal excitation and emission wavelengths at 358 and 455 nm, respectively. Additionally, the CQDs solution showed bright blue fluorescence under 365 nm UV light, with a quantum yield of 6.21% and a fluorescence lifetime of 3.02 ns. This study offers valuable insights into the green and efficient synthesis of fluorescent CQDs powder.

Tailored pore-confined single-site iron(III) catalyst for selective CH4 oxidation to CH3OH or CH3CO2H using O2.

Direct oxidation of methane to valuable oxygenates like alcohols and acetic acid under mild conditions poses a significant challenge due to high C‒H bond dissociation energy, facile overoxidation to CO and CO2 and the intricacy of C-H activation/C-C coupling. In this work, we develop a multifunctional iron(III) dihydroxyl catalytic species immobilized within a metal-organic framework (MOF) for selective methane oxidation into methanol or acetic acid at different reaction conditions using O2. The active-site isolation of monomeric FeIII(OH)2 species at the MOF nodes, their confinement within the porous framework, and their electron-deficient nature facilitate chemoselective C‒H oxidation, yielding methanol or acetic acid with high productivities of and , respectively. Experiments and theoretical calculations suggest that methanol formation occurs via a FeIII-FeI-FeIII catalytic cycle, whereas CH3CO2H is produced via hydrocarboxylation of in-situ generated CH3OH with CO2 and H2, and direct CH4 carboxylation with CO2.

Green Synthesis of New Spiropyrrole Derivatives by Employing Activated Carbonyl Compounds and Graphen Oxide Decorated on Ag@CuO/ZnO NPs or CuO/ZnO NPs: Theoretical and Biological Study

ABSTRACTThis study focused on investigating the synthesis of new spiropyrrole derivatives with high yields. The two nanocatalyst CuO/ZnO@Graphen Oxide nanocomposites (CuO/ZnO@GO NCs) and Ag/CuO/ZnO@Graphen Oxide (Ag@CuO/ZnO@GO NCs) were employed in a multicomponent reaction at room temperature to synthesize new compounds. The reaction involved isatins, N‐alkyl isatins, and ninhydrin as activated carbonyl compounds, ammonium acetate, ethyl 2‐arylamino‐4‐dioxo‐4‐arylbutanoates, hydrazonoyl chloride, or ethylbromopyruvate in water. Also, the catalytic activity of the green synthesized CuO/ZnO@GO and Ag@CuO/ZnO@GO was evaluated in the reduction of organic pollutants such as 4‐nitrophenol (4‐NP) in water at mild conditions. The results indicated that the biosynthesized MNCs have very high and effective catalytic activity for organic pollutants within a few seconds. The newly synthesized spiro compounds demonstrate antioxidant activity as a result of two assessment processes conducted by its NH group. In addition, the antibacterial activity of newly created spiro compounds was assessed using a disk distribution method, involving two types of Gram‐negative bacteria. These compounds were found to limit the growth of Gram‐positive bacteria as well. Also, to better understand the reaction mechanism, density functional theory (DFT)–based quantum chemical methods have been applied. The advantages of this technology encompass rapid response times, high product yields, and uncomplicated catalyst and product separation via simple procedures.

Techno-Economic Feasibility: Planning an On-Grid Solar Power System for Shrimp Pond Aeration

The rapid growth of the Indonesian shrimp farming industry is accompanied by high production costs, primarily driven by the reliance on fossil fuel-based energy sources that can destabilize the ecosystem. This study investigates the technical, economic, and environmental feasibility of using three energy sources, including photovoltaics (PV), grid, and generator, to supply aeration needs in shrimp ponds. A comparative analysis of three scenarios with on-grid schemes was conducted through optimization using Queen Honey Bee Migration (QHBM) and Grey Wolf Optimization (GWO) algorithms, namely Net Present Cost (NPC), Renewable Fraction (RF), and Carbon Emission (ECO2). From a technical point of view, a lower electricity tariff is obtained compared to the grid, which is US$ 589,968. The optimization results on the NPC, RF, and ECO2 parameters show that scenario 1 of the QHBM algorithm is the most optimal. This condition is evidenced by the acquisition of 3 parameters that are closest to the determination of the objective function, yielding an NPC of US$ 230,390.34, RF of 26.01%, and ECO2 of 1,484KgCO2e, with 655Wp PV specifications and the number of PV as many as 578pcs. Economically, the investment in a solar power plant for the shrimp pond obtained BEP of 4.2 years with a payback period (PP) obtained in year 5, net cash-flow of US$ 63,317.31, with ROI of 19% and NPV of US$ 775,159.40 in the same year.

Antimicrobial Peptides: A Promising Alternative to Conventional Antimicrobials for Combating Polymicrobial Biofilms.

Polymicrobial biofilms adhere to surfaces and enhance pathogen resistance to conventional treatments, significantly contributing to chronic infections in the respiratory tract, oral cavity, chronic wounds, and on medical devices. This review examines antimicrobial peptides (AMPs) as a promising alternative to traditional antibiotics for treating biofilm-associated infections. AMPs, which can be produced as part of the innate immune response or synthesized therapeutically, have broad-spectrum antimicrobial activity, often disrupting microbial cell membranes and causing cell death. Many specifically target negatively charged bacterial membranes, unlike host cell membranes. Research shows AMPs effectively inhibit and disrupt polymicrobial biofilms and can enhance conventional antibiotics' efficacy. Preclinical and clinical research is advancing, with animal studies and clinical trials showing promise against multidrug-resistant bacteria and fungi. Numerous patents indicate increasing interest in AMPs. However, challenges such as peptide stability, potential cytotoxicity, and high production costs must be addressed. Ongoing research focuses on optimizing AMP structures, enhancing stability, and developing cost-effective production methods. In summary, AMPs offer a novel approach to combating biofilm-associated infections, with their unique mechanisms and synergistic potential with existing antibiotics positioning them as promising candidates for future treatments.

Current Advances in the Photoconversion of Plastics: the Catalysts and Reaction Pathways.

Plastic waste has caused severe global environmental pollution and health issues due to the high production rate and lack of proper disposal technology. Traditional methods to deal with plastic waste, such as incineration and landfilling, are deemed unsustainable and energy-intensive. A promising alternative is the photocatalytic conversion of plastic waste, using sunlight as a sustainable and carbon-neutral energy source to break down plastic waste under ambient pressure and low temperatures. This review aims to provide a comprehensive summary of recent advancements in plastic photoconversion, with an emphasis on the catalysts and reaction pathways. The mechanisms and reaction pathways are first summarized, followed by a detailed discussion of strategies to design catalysts for improved performance in photoconversion. Then, examples of photothermal degradation processes are presented. Finally, current strategies, challenges, and possible future directions of plastic photoconversion are summarized and discussed.

Response of Onion to Deficit Irrigation Levels at Different Growth Stages on Yield and Water Productivity

The Ethiopian vegetable sub-sector, particularly onion, is crucial for the economy due to its high productivity and ease of cultivation. However, water scarcity in arid and semi-arid regions hinders its expansion. This study conducted at Wondo Genet Agricultural Research Center aimed to investigate the impact of deficit irrigation levels at various growth stages on onion yield and water productivity. The experiment employed a split plot design with four crop growing stages and three deficit irrigation levels. Results revealed that deficit irrigation significantly affected onion growth parameters and yield components. Bulb diameter, number of leaves per plant, and total bulb yield 5.84 cm, 6.85, 23.26 t/ha, respectively, were notably influenced by deficit irrigation levels, with the highest yields observed under full irrigation. Additionally, water use efficiency varied with deficit irrigation application timing, with the highest efficiency recorded during the maturation stage. Overall, deficit irrigation during initial and maturation stages at 55% ETc application level emerged as the most viable option, ensuring sustainable crop production in water-scarce areas. These findings underscore the importance of adopting deficit irrigation strategies to optimize water use efficiency and enhance onion yield in Ethiopia's vegetable sub-sector.

Screening, Gene Cloning and Expression of Cellulase-Producing Strain Bacillus subtilis Xh-16.

Cellulase is a complex enzyme system composed of multiple hydrolytic enzymes. It can degrade cellulose into glucose and improve the utilization efficiency of cellulose resources. Cellulase produced by microorganisms is the main method used in industry, offering the advantages of convenience and being environmentally friendly. A strain Xh-16 with high cellulase production was screened from the rotten rice straw. It was identified as Bacillus subtilis by morphological identification, physiological and biochemical identification, and 16S rRNA gene sequence analysis. Strain Xh-16 was used to degrade rice straw. After a 48h cellulase treatment, the complete degradation and structural breakdown of the straw were observed. We cloned the endoglucanase Cel5L gene of Bacillus subtilis Xh-16 and induced expression of the cloned gene in Escherichia coli BL21 (DE3). The results showed that the coding length of Cel5L gene was 1500bp, and the molecular weight of the encoded protein was about 55kDa. The molecular formula is C2456H3811N671O761S10 and it has 7709 atoms and 499 amino acids. Cel5L differs significantly from some the glycoside hydrolase family 5 cellulases because it has only one carbohydrate binding module family 3 at the C-terminus of its catalytic domain. The cellulase gene Cel5L in Xh-16 can encode active cellulase and can be heterologously expressed in Escherichia coli, which makes Escherichia coli have cellulase function. This study serves as a foundation for further research on cellulase diversity in Bacillus subtilis and offers insights for enhancing cellulase production.