Higher Protein Percentage Research Articles

Phytochemical profiling from Dioscorea bulbifera L. bulbils using LC-MS, proximate analysis and antidiabetic activity: in vitro and in silico approaches

ABSTRACT The nutritional value and medicinal properties of highly edible Bulbils of Dioscorea bulbifera L are still unexplored. This study reported the nutritional and medicinal values by In vitro, and In silico studies along with chemical profiling using LC-MS. The proximate analysis was conducted by the AOAC method. The antioxidant and antidiabetic activities were assessed using DPPH and α- α-glucosidase enzyme inhibition assay. Molecular docking was used to do an In silico examination of the identified molecules, and ADMET analysis of the hit candidate was also performed. The result showed that bulbils of D. bulbifera were found to have a higher percentage of protein (8.83%), fat (0.96%), and fiber (5.40%). It exhibited significant levels of phenolic and flavonoid content 106.93 ± 0.02 mg GAE/g and 11.51 ± 0.02 mg QE/g, respectively. The ethyl acetate fraction of D. bulbifera showed potent antioxidants with an IC 50 = 13.75 ± 0.6 μg/mL. Furthermore, the methanol extract showed more than 50 % inhibition activity with IC 50 values of 26.29 ± 0.6 μg/mL and 191.2 ± 0.7 μg/mL against α-glucosidase and α-amylase, respectively. The ethyl acetate fraction showed higher α-glucosidase and α-amylase inhibitory activity with an IC 50 of 14.42 ± 0.4 μg/mL and 171.5 ± 0.4 μg/mL, respectively, compared to the standard acarbose (p < 0.05). Chemical profiling of most bio-active ethyl acetate fraction was done using LC-MS analysis, where eleven compounds were annotated. In conclusion, bulbils of D. bulbifera demonstrate promising anti-diabetic activity through In vitro and In silico studies.

Yellow mealworm: effects of adults breeding density on adults and larvae performances from an industrial perspective

A key aspect to optimise the Tenebrio molitor (TM) farm productivity is to find an optimal breeding density for adults. To this purpose, this study investigated, from an industrial perspective, the impact of 4 breeding densities (D1, D2, D3 and D4, equal to 0.8, 1.1, 1.3 and 1.6 adults/cm2, respectively) on mortality and reproductive performances of TM adults and larvae. Two weeks after pupae emergence, TM adults were randomly assigned to the four groups and housed in 48 breeding crates (60 x 40 x 14.5 cm; 12 crates/group). The trial consisted in 4 consecutive weeks of adults breeding (each week represented one oviposition), and 8 weeks of larvae growth. From each week of oviposition, a batch of larvae was obtained for a total of 4 batches of larvae (48 crates/batch). Larvae of each batch were grown until 8 weeks of age, corresponding to the period required to reach the selling size. Larvae were kept in the original crates until the 5th weeks of age, after which they were divided into additional crates to ensure a density of 4.2 larvae/cm2 and a final weight of 1500 g of larvae/crate (0.6 g of larvae/cm2). For each oviposition week, TM adult’s mortality, eggs hatchability, BW, feed intake, chemical composition and fatty acids (FAs) profile were evaluated, while ovary weight was measured at weeks 1 and 4. The number of larvae was monitored at week 5 of age while their chemical composition at week 5 and 8. Larvae feed intake and BW were monitored over the 8-weeks growth period. Individual feed intake and BW of adults increased with increasing breeding density (P<0.0001 and P<0.05, respectively). With increasing adults breeding density, the number of larvae per crate increased (P<0.001) while the grams of larvae/gram of adult decreased (R2=0.8856). Larvae from groups D2, D3 and D4 showed higher feed intake per crate and higher individual and total final BW than D1 (P<0.001). However, the feed conversion ratio (FCR) worsened with increasing breeding density (P<0.001). D4 larvae had higher percentages of protein (P<0.001), lipids (P<0.05) and cholesterol (P<0.001) compared to larvae from other groups. Concluding, a breeding density of 0.8 adults/cm2 maximised both grams of larvae produced/grams of adults and larvae FCR. However, breeding densities above 0.8 adults/cm2, resulted in a higher number of produced larvae per crate with a greater final mass weight making a density of 1.6 adults/cm2 the preferable choice from and industrial perspective.

Analysis of physicochemical, functional, and antioxidant properties of four different Indian seaweed species

The present study aims to elucidate the nutritional, functional, flow, and antioxidant properties of Ulva fasciata, Ulva lactuca, Chaetomorpha linum, and Gracilaria edulis. The highest percentage of protein (24.26 %) in U. fasciata; fat (6.33 %) and total carbohydrates (46.39 %) in C. linum; crude fibre (8.67 %) in U. lactuca; ash (22.45 %) content in G. edulis was observed among the four-seaweed. The highest oil absorption capacity was found for C. linum (1.59 %), followed by G. edulis (1.21 %), U. lactuca (1.04 %), and U. fasciata (0.91 %). The water absorption capacity in U. fasciata (4.55 %) was observed to be the highest followed by C. linum (3.84 %), U. lactuca (3.78 %), and G. edulis (2.22 %). U. fasciata exhibited the highest solubility (35.83 ± 1.97 %) and emulsifying capacity (99.00 ± 0.00 %), while G. edulis showed the highest foaming capacity (75.00 ± 1.00 %). The angle of repose of G. edulis and C. linum powder showed excellent flowability than U. lactuca and U. fasciata. Carr's index and Hausner ratio showed that all seaweed samples were free-flowability, except for G. edulis and C. linum. The bulk density (0.71 g/cm3) and tapped density (0.85 g/cm3) of U. fasciata were observed to be the highest, whereas true density (1.11 g/cm3) was recorded highest in U. lactuca. The most increased antioxidant activity was found in G. edulis (65.90 %) followed by C. linum (53.01 %), U. lactuca (51.76 %), and U. fasciata (43.56 %). The total phenolic content (10.93 mg GAE/g) and flavonoid content (1.71 mg QE/g) were higher in U. fasciata. FTIR analysis detected the presence of amide, amino, hydroxyl, ester groups and it can conclude the presence of essential components such as proteins, amino acids, polysaccharides, and fat. Thus, the selected four marine seaweeds act as a good source of functional components and can be utilized for the development of functional food products such as bread, biscuits, cookies, tea, snacks, soups and beverages.

Genomic Selection of Large Yellow Croaker (Larimichthys crocea) with a High Plant Protein Diet Enhances the Growth Performance of Offspring.

Fishmeal is over-represented in the diets of large yellow croaker (Larimichthys crocea), and this farming mode, which relies heavily on fishmeal, is highly susceptible to the price of fishmeal and is unsustainable. Therefore, more and more studies on the large yellow croaker tend to replace fishmeal with land-based animal or plant proteins, but few studies have considered it from the genomic selection. In this study, we evaluated the survival rate (SR), final body weight (FBW), body weight gain (BWG), weight gain rate (WGR), and specific growth rate (SGR) of the large yellow croaker GS7 strain, which was obtained through genomic selection for tolerance to plant proteins and analyzed the differences in plant protein utilization between the GS7 strain and unselected commercial large yellow croaker (control group). The results of separate feeding for 60days showed that although there was no significant difference in SR between the control and GS7 strains (P > 0.05), the BWG, WGR, and SGR of the control were significantly lower (P < 0.05) than those of the GS7 group. Results of mixed feeding after PIT marking showed that compared to the control fish, the GS7 strain had significantly higher BWG, WGR, and SGR (P < 0.0001). To make the experimental results more precise, we compared fishes with equivalent initial body weight (IBW) in the GS7 strain and the control group. The final fish body weight (FBW) of Ctrl-2 (IBW 300-399g) and Ctrl-4 (IBW 500-599g) was significantly lower than those of the corresponding GS7-2 and GS7-4 (P < 0.05), while the FBW of Ctrl-1 (IBW 200-299g) and Ctrl-3 (IBW 400-499g) was much significantly lower than the corresponding GS7-1 and GS7-3 (P < 0.01). The BWG, WGR, and SGR of Ctrl-1 and Ctrl-4 were more significantly lower than those of the corresponding GS7-1 and GS7-4 (P < 0.01), while the BWG, WGR, and SGR of Ctrl-2 and Ctrl-3 were more significantly different from the corresponding GS7-2 and GS7-3 (P < 0.0001). Our results seem to point toward the same conclusion that the GS7 strain is better adapted to high plant protein diets than the unselected commercial large yellow croaker. These results will provide a reference for the low-fishmeal culture industry of large yellow croakers and the selection and breeding of strains tolerant to a high percentage of plant proteins in other marine fishes.

Productivity, seed quality and protein yield of pea samples developed by the FSBSI “ARC “Donskoy”

The purpose of the current study was to determine the quantitative and qualitative indicators of seeds of pea samples. The study was carried out in the southern part of the Rostov region in the fields of the FSBSI Agricultural Research Center “Donskoy” in 2021–2023. The objects of research were 12 pea samples developed by the ARC “Donskoy”. The zoned variety ‘Aksaisky Usatiy 5’ was used as a standard. Quality characteristics of peas, such as protein percentage in seeds and their ability to boil soft, are highly dependent on environmental conditions and often have a negative correlation with seed productivity. One of the goals in pea breeding is to change this inverse correlation between productivity and seed quality. The mean productivity of the varieties and lines in the Competitive Variety Testing through the years of study was 3.66 t/ha on average. The standard ‘Aksai Usatiy 5’ (3.46 t/ha) was reliably exceeded by such varieties and lines as ‘G-1181’ (4.17 t/ha), ‘Kazak’ (4.02 t/ha), ‘AKM’ (3.99 t/ha), ‘G-1313’ (3.80 t/ha). The samples with the highest protein percentage (second quality class, 22.0–25.0 %) were ‘Aksaysky Usatiy 5’ (23.7 %), ‘Skif’ (24.4 %), ‘Kazak’ (23.1 %), ‘AKM’ (23.6 %), ‘G-1141’ (24.3 %), ‘G-1172’ (25.0 %), ‘G-1181’ (22.3 %). The mean boiling time was 75 minutes. The minimum boiling time was 63 minutes for the varieties ‘Aksaisky Usatiy 5’, ‘Skif’, 65 minutes for the line ‘G-1234’ and 70 minutes for the line ‘G-1288’. The mean coefficient of the ability to boil soft over the years of study was 2.20, varying from 1.92 to 2.61. The mean protein yield over the years of study was 0.85 t/ha. The varieties ‘Kazak’ (0.94 t/ha), ‘AKM’ (0.93 t/ha) and breeding lines ‘G-1181’ (0.93 t/ha), ‘G-1313’ (0.90 t/ha) showed the best indicators. The maximum values of protein yield were identified at maximum productivity of 4.2–4.3 t/ha and 23.5–24.0% of protein in seeds.

Development and Physico-chemical analysis of value-added Chikki using iron rich garden cress seed and exploring its benefits in treatment of Anaemic patient

The effect of the incorporation of roasted garden cress seed powder (RGCSP) in the diet of girls with anaemia on Hb level and quality was evaluated. Twenty-two girls were selected and their Hb levels were estimated. Out of twenty-two girls, fifteen girls were found to be anemic and were selected for the study and it was found that four girls had mild, nine had moderate and two had severe degree of anaemia. The girls' average Hb levels dramatically raised from 8.9 to 10.4 g/dL following a 60-day intervention that also included RGCSP. Research on garden cress seed powder (GCSP) showed that roasting decreased the amount of moisture, protein, carbohydrate, and zinc while increasing the amount of ash, fat, crude fiber, and iron. Four distinct formulations were created with various amounts of GCSP (5 %, 10 %, 15 %, and 20 %) to generate fortified chikki. The chikki with 15 % GCSP had the highest overall acceptance rating of among them. Compared to the control chikki, this enriched variety had higher percentages of protein (6.95 %), fat (15.87 %), moisture (4.60 %), fiber (3.95 %), and calorie content (456.23 kcal/100 g). Supplemented chikki made by fortifying with RGCSP was rich in nutritional composition.

Integrated proteomic, phosphoproteomic, and N-glycoproteomic analyses of small extracellular vesicles from C2C12 myoblasts identify specific PTM patterns in ligand-receptor interactions

Small extracellular vesicles (sEVs) are important mediators of intercellular communication by transferring of functional components (proteins, RNAs, and lipids) to recipient cells. Some PTMs, including phosphorylation and N-glycosylation, have been reported to play important role in EV biology, such as biogenesis, protein sorting and uptake of sEVs. MS-based proteomic technology has been applied to identify proteins and PTM modifications in sEVs. Previous proteomic studies of sEVs from C2C12 myoblasts, an important skeletal muscle cell line, focused on identification of proteins, but no PTM information on sEVs proteins is available.In this study, we systematically analyzed the proteome, phosphoproteome, and N-glycoproteome of sEVs from C2C12 myoblasts with LC–MS/MS. In-depth analyses of the three proteomic datasets revealed that the three proteomes identified different catalogues of proteins, and PTMomic analysis could expand the identification of cargos in sEVs. At the proteomic level, a high percentage of membrane proteins, especially tetraspanins, was identified. The sEVs-derived phosphoproteome had a remarkably high level of tyrosine-phosphorylated sites. The tyrosine-phosphorylated proteins might be involved with EPH-Ephrin signaling pathway. At the level of N-glycoproteomics, several glycoforms, such as complex N-linked glycans and sialic acids on glycans, were enriched in sEVs. Retrieving of the ligand-receptor interaction in sEVs revealed that extracellular matrix (ECM) and cell adhesion molecule (CAM) represented the most abundant ligand-receptor pairs in sEVs. Mapping the PTM information on the ligands and receptors revealed that N-glycosylation mainly occurred on ECM and CAM proteins, while phosphorylation occurred on different categories of receptors and ligands. A comprehensive PTM map of ECM-receptor interaction and their components is also provided.In summary, we conducted a comprehensive proteomic and PTMomic analysis of sEVs of C2C12 myoblasts. Integrated proteomic, phosphoproteomic, and N-glycoproteomic analysis of sEVs might provide some insights about their specific uptake mechanism.

Identification of grain quality genes among wintercommon wheat varieties

Currently, the agricultural market offers a wide range of winter wheat varieties of domestic breeding. However, due to climate warming and the increasing frequency of arid years, it is urgent to develop varieties with wide adaptability, large productivity potential and grain quality. The purpose of the current study was to identify new genetic sources of winter common wheat with a complex of favorable gene alleles that control grain quality traits. In 2022–2023 there was studied the collection of 26 winter common wheat varieties from breeding institutions of the Rostov region and Krasnodar region using generally accepted breeding techniques. There was carried out an analysis of technological quality traits and identification of loci associated with these traits using KASP-markers developed at the Institute of Plant Biology and Biotechnology (Kazakhstan). The varieties developed by the Federal Rostov ARC generated the largest productivity (341 g/m2) with less accumulation of protein and gluten (14.2 and 29.5 %), and, on the contrary, the varieties of the ARC “Donskoy” and the National Grain Center named after P. P. Lukyanenko were characterized by higher percentage of protein and gluten (15.5–15.7 and 33.2–34.4 %), but lower productivity (244–276 g/m2). The varieties ‘Donskoy Mayak’, ‘Konkurent’, ‘Nakhodka’, ‘Rostovchanka 7’, ‘Duplet’ and the line ‘K 18918’ were distinguished by the best technological traits of grain (protein &gt;15 %, gluten &gt; 30 %, gluten index &gt; 85 % and sedimentation ≥ 53 %) and many favorable alleles of SNP loci associated with these features. The varieties ‘Donna’, ‘Zolushka’, ‘Donskaya Lira’ exceeded the standard ‘Omskaya 4’ (368–378 g/m2) in productivity, but their allelic variants of the identified markers were lower. There have been identified the varieties ‘Donna’, ‘Zolushka’, ‘Donskaya Lira’ (Federal Rostov ARC), ‘Donskoy Mayak’, ‘Konkurent’, ‘Nakhodka’, ‘Rostovchanka 7’ (ARC “Donskoy”), ‘Duplet’, ‘line K 18918’ (“National Grain Center named after P. P. Lukyanenko”) with the maximum number of favorable alleles (10–11), associated with technological quality traits, recommended for improving winter wheat varieties in terms of grain quality.

Dietary CP and digestion kinetics influence BW loss, litter weight gain, and reproduction by affecting postprandial amino acid metabolism in lactating sows

To avoid a high body protein mobilization in modern lean sows during lactation, an adequate dietary amino acid (AA) supply and an efficient AA utilization are crucial. This study evaluated the effects of dietary CP and in vitro protein digestion kinetics on changes in sow body condition, litter weight gain, milk composition, blood metabolites, protein utilization efficiency and subsequent reproductive performance. We hypothesized that a slower digestion of dietary protein would improve AA availability and utilization. In total, 110 multiparous sows were fed one of four lactation diets in a 2 × 2 factorial design, with two CP concentrations: 140 g/kg vs 180 g/kg, and two protein digestion kinetics, expressed as a percentage of slow protein (in vitro degradation between 30 and 240 min): 8 vs 16% of total protein. Feeding sows the high CP diets reduced sow weight loss (Δ = 7.6 kg, P < 0.01), estimated body fat loss (Δ = 2.6 kg, P = 0.02), and estimated body protein loss (Δ = 1.0 kg, P = 0.08), but only at a high percentage of slow protein. A higher percentage of slow protein increased litter weight gain throughout lactation (Δ = 2.6 kg, P = 0.04) regardless of CP concentrations, whereas a higher CP only increased litter weight gain during week 3 of lactation (Δ = 1.2 kg, P = 0.01). On Day 15 postfarrowing, serial blood samples were taken from a subsample of sows fed with the high CP diets. In these sows, a high percentage of slow protein resulted in higher plasma AA concentrations at 150 and 180 min after feeding (Δ = 0.89, P = 0.02, Δ = 0.78, P = 0.03, mmol/L, respectively) and lower increases in urea at 90 and 120 min after feeding (Δ = 0.67, P = 0.04, Δ = 0.70, P = 0.03, mmol/L, respectively). The higher dietary CP concentration increased total nitrogen loss to the environment (Δ = 604 g, P < 0.01) with a reduction of protein efficiency (Δ = 14.8%, P < 0.01). In the next farrowing, a higher percentage of slow protein increased subsequent liveborn litter size (Δ = 0.7, P < 0.05). In conclusion, feeding sows with a high dietary CP concentration alleviated maternal weight loss during lactation when the dietary protein digestion rate was slower, but lowered protein efficiency. A slower protein digestion improved litter weight gain, possibly by reducing AA oxidation and improving plasma AA availability, thus, improving protein efficiency.

A Novel Bacillus amyloliquefaciens Specifically Improving the Solubility and Antioxidant Activities of Edible Bird's Nest.

Edible bird's nest (EBN), a most highly priced and valuable foodstuff, contains high percentage of proteins and carbohydrates. However, proteins adhering to these carbohydrates make the EBN hard and tough, which need to be boiled as the bird's nest soup to make the Chinese cuisine. To overcome the hard and tough texture of EBN and improve the digestion degrees, the present study screened and identified a probiotic strain Bacillus amyloliquefaciens YZW02 from 5-year stored EBN sample completely solubilizing EBN for the first time. The 24-h B. amyloliquefaciens fermented EBN contained 20.30-21.48mg/mL of the soluble protein contents with a recovery rate of 98-100%, DPPH radical scavenging rate of 84.76% and ABTS radical scavenging capacity of 41.05%. The mixed fermentation of B. amyloliquefaciens YZW02 and Bacillus natto BN1 were further applied to improve the low-MW peptide percentages and antioxidant activities. The mixed-fermentation of B. natto BN1 with 4-h cultured B. amyloliquefaciens YZW02 had the lowest percentage (82.23%) of >12-kDa proteins/peptides and highest percentages of 3-12kDa, 1-3kDa and 0.1-1kDa peptides of 8.6% ± 0.08, 7.57% ± 0.09, 1.77% ± 0.05 and 0.73% ± 0.05, with the highest DPPH, ABTS and •OH scavenging capacity of 90.23%, 46.45% and 49.12%, respectively. These findings would provide an efficient strategy for improving the solubility and antioxidant activities of EBNs.

Nutritional and Organoleptic Characteristics of Sausage Based on an Autochthonous Ecuatorian Fish, Old Blue (Andinoacara rivulatus).

The consumption of fisheries and aquaculture products has been increasing in recent decades, and it is necessary to strike a balance between production and sustainability in aquaculture; this is essential homework to support the demand for human food. This study aimed to investigate the sensory and nutritional characteristics of a sausage made from the fillet of the native fish Old Blue (Andinoacara rivulatus) to stimulate the local economy. A multifactorial A*B*C design was used, with Factor A being rearing systems (wild and farmed), Factor B being types of protein (quinoa meal and soybean meal), and Factor C being lipids of animal (pork fat) and vegetable (sunflower oil) origin. Highly significant differences were observed in all formulas, according to Tukey (p < 0.05). The highest protein percentage was with soybean flour at 11.24%, while quinoa flour had 10.80% of the product. In sensory characteristics, the best attributes were for texture in the mouth with a hedonic scale from 0 to 5, with 4.2 firmness being mostly acceptable, odor at 4.5, the aromatic attribute with the highest and best value, and color was the clearest at 4.3 acceptability. The flavor was 4.3, mostly more pleasant according to the tasters. The yield of farmed fillets was 23.16% compared to wild 13.89%, and the balance of sausage yield was 393 g with a yield of 76.33% of the total weight. Microbiological analysis of the sausage with the native species showed the absence of pathogenic microorganisms. A commercial shelf life of 30 days was also established for the sausage, demonstrating an added value through this processing, allowing its exploitation in areas further away from the fishing sites and, thus, a greater possibility of social development in the area.

Harnessing the power of new genetic tools to illuminate Giardia biology and pathogenesis.

Giardia is a prevalent single-celled microaerophilic intestinal parasite causing diarrheal disease and significantly impacting global health. Double diploid (essentially tetraploid) Giardia trophozoites have presented a formidable challenge to the development of molecular genetic tools to interrogate gene function. High sequence divergence and the high percentage of hypothetical proteins lacking homology to proteins in other eukaryotes have limited our understanding of Giardia protein function, slowing drug target validation and development. For more than 25 years, Giardia A and B assemblages have been readily amenable to transfection with plasmids or linear DNA templates. Here, we highlight the utility and power of genetic approaches developed to assess protein function in Giardia, with particular emphasis on the more recent clustered regularly interspaced palindromic repeats/Cas9-based methods for knockdowns and knockouts. Robust and reliable molecular genetic approaches are fundamental toward the interrogation of Giardia protein function and evaluation of druggable targets. New genetic approaches tailored for the double diploid Giardia are imperative for understanding Giardia's unique biology and pathogenesis.

The proximate composition and phytochemical screening of Momordica Balsamina (balsam apple) fruit and leaves

Malnutrition is a global issue that affects both children and adults irrespective of their socio-economic status. It is therefore important to find various means to tackle malnutrition. This is especially important as undernutrition and overnutrition can be linked to a variety of non-communicable diseases (NCDs). Therefore, this study aimed to gather more insight into the nutritional and phytochemical quality of Momordica balsamina leaves and fruit (fruit pericarp, fruit flesh and seeds). The results showed that M. balsamina had a nutritional composition that would be advantageous to the human diet. The nutritional quality was verified by the presence of a high protein percentage across all samples (19.72–29.08%), with the leaves containing the highest protein content (29.08% ± 0.77). There was also a low-fat content present across all samples which ranged from 1.03% to 2.40%. The ash content indicated the presence of total minerals to be adequate (2.93–21.16%), where the pericarp had the highest ash quantity (21.16% ± 0.09). Overall, the moisture levels were low (7.11–13.40%); with M. balsamina seeds containing the highest carbohydrate content (67.84% ± 0.30). Moreover, rich in the major phytoconstituents, M. balsamina extracts were found to contain alkaloids, saponins, cardiac glycosides, steroids and triterpenoids. Based on these findings, it can be deduced that the incorporation of M. balsamina into an individual’s diet could prevent diseases associated with malnutrition and could be used to supplement the human diet in managing certain NCDs.

Role of Nutrient Management in Yield, Quality and Nutrient Content of Egyptian Clover (Trifolium alexandrinum L.) Under Calcareous Soil Conditions

Nutrient management is vital for maximizing forage crop yield and economic viability; however, improper application can result in decreased yield and quality. Therefore, two seasonal experiments were conducted during the winter seasons of 2022-2023 and 2023-2024 to examine the impact of varying nitrogen, phosphorus and potassium (NPK) levels, along with the foliar application of zinc, on the forage yield and nutrient content of Egyptian clover or berseem (Trifolium alexandrinum L.) grown in calcareous soil conditions (&gt;36% CaCO3). The experiment consisted of eight treatments, including two NPK levels (50% and 100% of the recommended dose) and two forms of Zn (Zn NPs and Zn Edta at 0.2 and 0.5% as foliar sprays), compared with the control (without treatment). Results showed that NPK full dose (100%) or in combination with Zn NPs significantly improved fresh and dry weight by an average of 67%, and 80%, respectively, across all cuts. Whereas, the highest protein percentage in berseem forage occurred with 50% NPK and foliar Zn NPs. Protein yield per unit area was highest with 100% NPK and foliar Zn NPs in the second and third cuts. Furthermore, Zn NPs boosted manganese (Mn) levels in the 2nd cut but decreased them in the 3rd cut. Iron (Fe) and Mn were mostly in normal ranges, but zinc was often below normal levels. Overall, Zn NPs increased forage yields. The results of translocation factor (TF) for micronutrients from the soil to berseem plants showed that TF values were &gt; 1 for all micronutrients except for Cu in several instances in the 2nd cut in such calcareous soil. Furthermore, micronutrient translocations were arranged in the following order Cu &lt; Zn &lt;Mn &lt;Fe in the 2nd and 3rd cuts. Our study suggested that there is a beneficial role of correcting Zn deficiency under calcareous soil conditions which is reflected on berseem yield and quality and nutrient status. Thus, using ZnO NPs along with the full recommended dose of NPK is a suitable approach to enhance berseem crop yield and quality in calcareous soil.

Investigation of the peptides with calcium chelating capacity in hydrolysate derived from spent hen meat.

Calcium peptide chelates are developed as efficient supplements for preventing calcium deficiency. Spent hen meat (SHM) contains a high percentage of proteins but is generally wasted due to the disadvantages such as hard texture. We chose the underutilized SHM to produce peptides to bind calcium by proteolysis and aimed to investigate chelation between calcium and peptides in hydrolysate for a sustainable purpose. The optimized proteolysis conditions calculated from the result of response surface methodology for two-step hydrolysis were 0.30% (wenzyme/wmeat) for papain with a hydrolysis time of 3.5h and 0.18% (wenzyme/wmeat) for flavourzyme with a hydrolysis time of 2.8h. The enzymatic hydrolysate (EH) showed a binding capacity of 63.8±1.8mg calcium/g protein. Ethanol separation for EH improved the capacity up to a higher value of 68.6±0.6mg calcium/g protein with a high association constant of 420M-1 (25°C) indicating high stability. The separated fraction with a higher amount of Glu, Asp, Lys, and Arg had higher calcium-binding capacity, which was related to the number of ─COOH and ─NH2 groups in peptide side chains according to the result from amino acid analysis and Fourier transform infrared spectroscopy. Two-step enzymatic hydrolysis and ethanol separation were an efficient combination to produce peptide mixtures derived from SHM with high calcium-binding capacity. The high percentage of hydrophilic amino acids in the separated fraction was concluded to increase calcium-binding capacity. This work provides foundations for increasing spent hen utilization and developing calcium peptide chelates based on underutilized meat.

New pea variety ‘Antey’

The current paper has presented the characteristics of the new pea variety ‘Antey’ of Altai breeding according to the most important economically valuable and morphological characteristics. Breeding work to develop a variety at the FSBSI “Federal Altai Research Center of Agrobiotechnologies” began in 2003 from the moment of hybridization of the original parental forms. The variety ‘Antey’ was developed by individual selection from a hybrid combination (Varyag x Novosibirets) x Batrak. The species of the variety was ecaducum. On average, over the years of testing (2020–2022), the variety ‘Antey’ reliably differed from the standard variety ‘Batrak’ in terms of a set of breeding valuable traits. This variety has the most important economic and biological indicators, such as high productivity (2.75 t/ha), resistance to lodging (5 points), low susceptibility to diseases (4.5 points), high protein percentage (23.5 %). Based on phenological observations of pea crops, there have been identified some morphological features of this variety. The variety ‘Antey’ is a middle maturing one. The vegetation period lasts 70...75 days. The variety is determinate, plant height is 65 cm. The leafless type and compressed internodes of the new pea variety increase the resistance of shoots to lodging. The plants bear white flowers of medium size. Beans are shelled, straight. The number of seeds in a bean is 4–7. Our research has shown that over the course of three years in the Competitive Variety Testing, according to the above-mentioned traits, the variety ‘Antey’ significantly exceeds those of the standard variety ‘Batrak’, which made it possible to send this pea variety to the State Variety Testing in 2022. The variety ‘Antey’ is going to be tested in region 10 (West Siberian).

Pumpkin seed flour: physical, physicochemical and chemical characteristics

Around 1.3 billion tons of food are thrown away every year in the world, and it is estimated that 60 kg/per capita are wasted in Brazil. As a result, the UN's 2030 schedule includes among the 17 Sustainable Development Goals (SDGs), number 12, which deals with responsible consumption and production, involving the social, environmental, economic and institutional dimensions. By using food in its entirety and/or its fractions (peels, seeds, stalks and leaves), there is a reduction in waste, environmental impacts and food production costs. Pumpkin seeds are nutritionally rich and can be used in many different applications, including as flour. Pumpkin Seed Flour (PSF), produced from Cucurbita maxima L., was evaluated for yield, physical, physicochemical and chemical characteristics. The flour yield was 93.9%, the water absorption index confirmed the low presence of starch and the solubility index confirmed the presence of soluble solids. In composition, it presented a high percentage of fibers, lipids and proteins, characteristic of oilseeds and legumes. FSA does not contain gluten and can nutritionally enhance foods for celiac patients.

Associations of bovine beta-casein and kappa-casein genotypes with genomic merit in Holstein Friesian cattle

Abstract. The relationship between the index values used to evaluate the genomic value and essential markers such as casein genes provides important information at the herd level. Therefore, this study aimed to determine the association between casein gene genotypes and the indices of genetic merit in Holstein Friesian cattle. A total of 805 cows were genotyped using the Affymetrix® Axiom® array system. We used data composed of the total performance index (TPI) and net merit (NM) values as well as the predicted transmitting abilities (PTAs) indices, including milk, fat, fat percentage, protein, protein percentage, combined fat and protein (CFP), productive life (PL), somatic cell score (SCS), daughter pregnancy rate (DPR), livability (LV), udder composite (UDC), and feet–legs composite (FLC) of each animal. The statistical analysis consisted of a one-way analysis of variance (ANOVA) test followed by Tukey's test. The A2A2 and the AB genotypes were predominant in the CSN2 and CSN3 genes, respectively. The A2A2 animals were found to have higher TPI and NM values. Moreover, they exhibited higher PTA values for proteins, CFP, and PL. On the other hand, the A1A1 genotype was significantly associated with the highest UDC. Concerning the CSN3 haplotypes, the BB animals had higher protein percentage and PL than alternative haplotypes. The AA and AB haplotypes were found to be significantly associated with the highest SCS and DPR values, respectively. In addition, the BE haplotype had the highest NM. Selection procedures focusing on casein genes in dairy cattle are becoming increasingly common worldwide, especially for A2 milk. However, herd-based dynamics are also fundamental to providing a desired genetic merit for the animals. This study may be valuable for further analyses regarding selection decisions using the breeding values of candidate animals in commercial dairy herds.

Acetylomics reveals an extensive acetylation diversity within Pseudomonas aeruginosa.

Bacteria employ a myriad of regulatory mechanisms to adapt to the continuously changing environments that they face. They can, for example, use post-translational modifications, such as Nε-lysine acetylation, to alter enzyme activity. Although a lot of progress has been made, the extent and role of lysine acetylation in many bacterial strains remains uncharted. Here, we applied stable isotope labeling by amino acids in cell culture (SILAC) in combination with the immunoprecipitation of acetylated peptides and LC-MS/MS to measure the first Pseudomonas aeruginosa PAO1 acetylome, revealing 1076 unique acetylation sites in 508 proteins. Next, we assessed interstrain acetylome differences within P. aeruginosa by comparing our PAO1 acetylome with two publicly available PA14 acetylomes, and postulate that the overall acetylation patterns are not driven by strain-specific factors. In addition, the comparison of the P. aeruginosa acetylome to 30 other bacterial acetylomes revealed that a high percentage of transcription related proteins are acetylated in the majority of bacterial species. This conservation could help prioritize the characterization of functional consequences of individual acetylation sites.

Effect of Some Fertilizer Combinations of Nitrogen, Phosphorus and Potassium on Some Qualitative Characteristics of Quinoa in Seed

A field experiment was carried out during the season 2020/2021 in Anbar Governorate - Heet district - Sweeb region to study the effect of levels of nitrogen, phosphorus and potassium on some yield characteristics of quinoa plants using three levels of nitrogen (50, 100, 150) kg h−1 and symbolized by (N1, N2, N3) respectively and three levels of phosphorus (22, 44 and 66) kg P ha−1 and took the symbolized (P1, P2, P3) and three levels of potassium (33.2, 66.2, 99.2) kg K.ha−1 The experiment was designed according to the randomized complete block design (RCBD) with three replications. The results of the experiment showed that the highest percentage of protein in quinoa grains reached 16.50% at low fertilizer levels of nitrogen and high levels of phosphorus and potassium when treated N1P3K3, As for the oil percentage, the low levels of nitrogen, phosphorus and potassium gave the highest oil percentage of 4.39%, as well as the phenols gave the highest value of 74.73 mg Gallic gm−1 at medium levels of nitrogen and low levels of potassium and phosphorus 34.20 mg Gallic gm−1 when treated N2P1K1. As for the flavonoids, it gave the highest value of 34.20 mg Rutin gm−1 It was found that there was a strong positive correlation between phenols and flavonoids of r = 0.78. As for saponins it increased with a decrease in the level of nitrogen and an increase in the levels of phosphorus and potassium, as it gave the highest value of 8.21%, that is, when treating N2P1K1, and it was found that there was a strong negative correlation between The percentage of saponins and the percentage of nitrogen in seeds had a value of r=-0.75.