Highest Germination Percentage Research Articles

Effect of Different Seed Treatment on Germination of Fishtail Palm (Caryota urens) under Naturally Ventilated Poly-house Conditions

An investigation was conducted to study the effect of different seed treatments comprising of mechanical scarification, chemical and plant growth regulator on germination of Fishtail palm (Caryota urens) seeds under naturally ventilated poly-house at sheth D. M. Polytechnic in Horticulture College, Anand Agricultural University, Vadodara during 2021 to 2023. There were total 12 treatments under study which was replicated trice and statistically analysed by completely randomized design at 90 days after sowing. The results revealed that treatment T12 i.e. seeds treated with GA3 1000 ppm for one day recorded significantly lesser days to initiation of germination (46.33 days) along with higher germination percentage (86.67 % and 85.00 %), germination rate index (5.90 %/day and 5.70 %/day) and germination index (255 and 243.67), coefficient of velocity of germination (6.18 and 6.02), timpson’s germination index (1275 and 1218), modified timpson germination index (42.50 and 40.61) and survival percentage (88.33 and 86.67) at 90 days after sowing during first and second year respectively. While, seed without any treatment kept in open condition treatment T1 showed poor performance in germination percentage as well as survival percentage. Thus, Fishtail palm (Caryota urens) seeds soaked in solution of 1000 ppm GA3 along with sowing them in polybags and kept under NVPH condition provides better germination and survival.

Molecular characterization of plant growth-promoting Trichoderma from Saudi Arabia.

Fungi in the genus Trichoderma are widespread in the environment, mainly in soils. They are used in agriculture because of their mycoparasitic potential; Trichoderma have the ability to increase plant health and provide protection against phytopathogens, making them desirable plant symbionts. We isolated, identified, and characterized Trichoderma from different regions of Saudi Arabia and evaluated the ability of Trichoderma to promote plant growth. Morphological and molecular characterization, along with phylogenetic studies, were utilized to differentiate between Trichoderma species isolated from soil samples in the Abha and Riyadh regions, Saudi Arabia. Then, plant growth-promoting traits of the isolated Trichoderma species were assessed. Eight Trichoderma isolates were characterized via morphological and molecular analysis; six (Trichoderma koningiopsis, Trichoderma lixii, Trichoderma koningii, Trichoderma harzianum, Trichoderma brevicompactum, and Trichoderma velutinum) were from Abha and two (T. lixii and T. harzianum) were from Riyadh. The isolated Trichoderma strains belonged to three different clades (Clade 1: Harzianum, Clade 2: Brevicompactum, and Clade 3: Viride). The Trichoderma isolates varied in plant growth-promoting traits. Seeds treated with most isolates exhibited a high percentage of germination, except seeds treated with the T3-T. koningii isolate. 100% germination was reported for seeds treated with the T4-T. harzianum and T6-T. brevicompactum isolates, while seeds treated with the T1-T. koniniopsis and T5-T. lixii isolates showed 91.1% and 90.9% germination, respectively. Seeds treated with the T8-T. velutinum, T2-T. lixii, and T7-T. harzianum isolates had germination rates of 84.1%, 82.2%, and 72.7%, respectively. The Trichoderma isolate T5-T. lixii stimulated tomato plant growth the most, followed by T7-T. harzianum, T8-T. velutinum, T4-T. harzianum, T1-T. koniniopsis, T2-T. lixii, and T6-T. brevicompactum; the least effective was T3-T. koningii. A maximum fresh weight of 669.33mg was observed for the T5-T. lixii-treated plants. The Abha region had a higher diversity of Trichoderma species than the Riyadh region, and most isolated Trichoderma spp. promoted tomato growth.

Impact of dielectric barrier discharge plasma and plasma-activated water on cotton seed germination and seedling growth

Unfavorable environmental conditions during planting can reduce seed germination and hinder seedling growth. To address this issue, manufacturers are exploring innovative and cost-effective methods, such as cold plasma discharge. This simple, low-cost, and efficient physical technique induces significant biological responses in seeds and plants without the use of traditional, environmentally hazardous chemicals. This study investigated the impact of dielectric barrier discharge (DBD) plasma and plasma-activated water (PAW), produced by gliding arc plasma, on the germination and seedling growth of My344 cotton seeds. The seeds were pre-treated with 80 W of DBD plasma for 0, 1, 2, and 3 min, and subsequently soaked for 90 min in PAW with varying pH levels of 5.82, 3.88, 3.63, and 3.38. The results showed that plasma treatment positively influenced seed germination and seedling growth. The highest germination percentage (98.89 %) was observed with 1 min of DBD treatment, followed by PAW priming at pH levels of 3.63 and 3.38. Additionally, a 3-min DBD treatment followed by soaking in PAW with a pH of 3.63 led to significant increases in stem length (76.76 %), root length (48.77 %), and wet weight (76.44 %). Furthermore, it was observed that the electrical conductivity of the seeds in all groups decreased significantly with increased PAW acidity. The physical and chemical effects of cold DBD plasma on the seed surface, as well as changes in hydrophilicity, were further examined using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and water contact angle imaging.

Physiological quality of green corn seeds under water stress conditions

This study aimed to evaluate the physiological quality of green corn seeds subjected to water stress. The experimental design used was completely randomized, in a 2 x 5 factorial arrangement, consisting of two corn hybrids (AG1051 and BRS3046) and five osmotic potentials (0; -0.4; -0.8; -1.2 and – 1.6 MPa) simulated with aqueous solutions of polyethylene glycol 6000 (PEG 6000), with four replications of 50 seeds per treatment, totaling 40 experimental units. The analyzed variables were: water content, germination, root protrusion, final root protrusion, germination speed index, root length and coleoptile length. There was a significant effect of the interaction between hybrid factors and osmotic potentials for all analyzed variables, except for final root protrusion, which showed an isolated effect for the two factors studied. The results obtained indicated a higher percentage of germination and initial development of green corn seedlings for green corn seeds, hybrids AG1051 and BRS3046 at the osmotic potential of 0.0 MPa. The germination and vigor of green corn seeds decrease under water stress conditions from the osmotic potential of -0.4 MPa induced by PEG 6000, inhibiting the development of normal seedlings, affecting seed viability. Hybrid AG1051 presents superior germination and vigor compared to BRS3046.

Pre-Germinative Treatments and In Vitro Germination of Dianthus caryophyllus and Alstroemeria spp. Seeds

Alstroemeria spp. and carnation (Dianthus caryophyllus L.) have considerable and increasing economic importance in the floriculture market, therefore breeders carry out intense breeding programs to select new superior varieties. However, poor germination of hybrid seeds remains a bottleneck. Based on this assumption, seed pre-treatments and in vitro germination protocols, using different germination substrates, were applied in Alstroemeria spp. and carnation to improve germinability. Seed viability was tested using the 2,3,5-triphenyl tetrazolium chloride (TTC) test, and resulted in 91.10% ± 2.33 and 86.66% ± 3.85 in Alstroemeria and carnation, respectively. In Alstroemeria, pre-treatment with potassium nitrate (KNO3) in combination with modified ½ Murashige and Skoog (MS) medium ensured high germination uniformity combined with high germination percentage, showing significantly higher values than the control. In carnation, a suitable seed sterilization procedure was set up (up to 95.8% sterility); treatments with gibberellic acid (GA3) and KNO3 did not influence germination percentage compared to the control. A high multiplication rate of seedling lines was obtained on hormone-free MS medium.

Isolation, morphological characterization, and screening virulence of Beauveria bassiana and Metarhizium robertsii fungal isolates on Galleria mellonella

Background Entomopathogenic fungi exists naturally in plants as an asymptote and have the potential to reduce the population of insect pests through indirect interactions. This study was conducted to detect and characterize the endophytic fungi Beauveria bassiana and Metarhizium robertsii from the rhizosphere soil of tomato plants and their virulence effect on Galleria melonella. Methods From the rhizosphere soil of 40 tomato fields, three Beauveria bassiana and seven Metarhizium robertsii isolates were isolated using the galleria bait method. All fungi isolate were morphologically characterized by their colony color, shape, and surface texture. Isolates with the highest percentages of germination, conidial yield, and radial growth were selected, and their virulence was evaluated on second instar larvae of Galleria melonella under laboratory conditions. Results In this study, Beauveria bassiana showed white colony color and aseptate hyphae, whereas Metarhizium robertsii showed dark green to light green colony color and septate hyphal structures. Maximum spore production and conidial length were obtained by Beauveria bassiana isolate APPRC-27 with 2.67x107 spores ml-1 and 3.24 µm, respectively. Colony radial growth rates ranged from 1.73 to 3.24 mm day-1. The results revealed that the highest mortality rate of Galleria melonella (100%) was obtained by Metarhizium robertsii isolates K-61 and K-102 at a concentration of 1x108 conidial ml-1 at 7 days post-inoculation. The lowest mortality rate was registered by Metarhizium robertsii isolate RST-11. Conclusions In the present study, isolates that produced the most spores and had the highest germination rates were the most virulent to Galleria mellonella second instar larvae. Therefore, virulent entomopathogenic fungi, Beauveria bassiana and Metarhizium robertsii, are promising bioagents for the control of insect pests.

The Effects of Organic Fertilizers and NPK on Growth and Yields of Cowpea (Vigna unguiculata. L) in Kano Plains Kisumu, Kenya

Excessive use of inorganic mineral fertilizers as an agronomic practice has resulted in the depletion of soil organic matter of soils of many regions thereby rendering such soils infertile and unable to maintain sustainable production. The use of organic amendments provides the immediate strategy for compensating for soil carbon depletion thereby reducing land degradation. The purpose of this study was to evaluate the effects of six organic amendments on the growth and yield of Cowpea (Vigna unguiculata L) as compared to the conventional use of inorganic fertilizer NPK. The organic amendments were Boom Max, Ecoplanting, Evergrow, Dung slurry, Filter mud, and Market waste slurry applied to the soil at 8.33 t/ha against NPK (9:15:20) at 250 kg/ha. The experiment was laid in a RCBD. Data on growth and yields was collected on 10 tagged plants per plot. The results showed differences among treatments. Mature composts (Boom Max, Ecoplanting, and Evergow) resulted in a significantly (p< 0.05) higher germination percentage (53.49% on average) compared to that of Filter mud (41.50%) and the slurries (34.18% on average) while all the composts resulted in 25% higher number of branches on the average in season two as compared to the bio-slurries. In season three, Boom Max gave the highest germination percentage (41.02%) which was significantly higher than those of Evergrow (29.80 %), Filter mud and Dung slurry (27.18% on average) and NPK and Market waste slurry (5.64% on average) but not different from that of Ecoplanting. Five of the amendments had significantly better germination rates than NPK by an average of 20%. There were no significance differences (P≤0.05) in growth parameters between the mature composts and the NPK fertilizer, suggesting that the mature composts were equally efficient in nutrient supply to the crop as did the NPK fertilizer for plant growth. The application of organic amendments produced significant improvements in fresh leaf yields of the cowpea crop, with the highest yield recorded from the Boom Max applied plot (5781 kg on average), which was not different from others in seasons one and three but significantly different from that of NPK in season three. It can be concluded that depending on choice or type of amendment, organic manures are capable of producing similar or better results of crop growth and yields as the inorganic fertilizers.

Seed Priming and Biopriming in Two Squash Landraces (Cucurbita maxima Duchesne) from Tunisia: A Sustainable Strategy to Promote Germination and Alleviate Salt Stress.

In recent years, seed priming has gained interest, with researchers aiming to enhance seed germination and early growth, especially under abiotic stress conditions. In this study, seeds from two squash landraces (Cucurbita maxima Duchesne; i.e., Galaoui large seeds (Galaoui hereafter) and Batati green (Batati hereafter)) were subjected to different priming methods ((a) 0.3% and 0.4% KNO3 (halopriming); (b) 0.1% and 0.2% GA3 (hormopriming); (c) inoculation with Trichoderma spp. (T. harzianum, T. viride, and T. virens), Bacillus subtilis, and Pseudomonas fluorescens (biopriming) in order to promote germination parameters and seedling growth under salinity stress (0, 100, and 200 mM of NaCl). Our findings indicate the better performance of primed seeds compared to the untreated ones in terms of germination and seedling growth traits, although a varied response depending on the priming method and the landrace was observed. The highest germination percentage (GP) and the lowest mean germination time (MGT) were observed in 0.4% KNO3-primed seeds. The positive effects of 0.4% KNO3 were also depicted in all traits related to seedling growth and the seedling vigor index (SVI), indicating its effectiveness as a priming agent in squash seeds. Under salinity stress conditions, priming with 0.4% KNO3 significantly improved the germination and seedling growth traits for both landraces, while the application of 0.2% GA3 at high salinity significantly improved photosynthetic quantum yield (Fv/Fm ratio). Regarding the effects of biopriming in germination and seedling growth traits, our results indicate that T. harzianum and B. subtilis were the most effective bioagents in promoting germination and seedling growth in Galaoui and Batati seeds, respectively. In conclusion, our findings provide important information regarding the practice of using priming and biopriming agents to enhance the germination and seedling growth capacity of squash seeds, as well to mitigate the negative effects of salinity stress at the critical stages of germination and early growth.

Effect of D-mannitol and Gibberellic Acid on Physiological Parameters, Early Seedling Growth Characteristics, and Some of the Most Important Chemical Components of Barberry (Berberis vulgaris L.)

Background: One of the important medicinal plants and agricultural products in Asia, particularly in Iran, is barberry, and both salinity and drought can cause osmotic or ionic imbalance in plant cells. In order to study the impacts of D-Mannitol and gibberellic acid on seed germination, physiological parameters, early seedling growth characteristics, and some of the most important chemical ingredients of barberry, one experiment is designed. Methods: The experimental design was Completely Randomized Design (CRD) with three replicates, and drought stress was done by D-mannitol at various stress levels, namely, 0 MPa, 40 MPa, and 80 MPa, and the reaction of studied cultivar was assessed on the basis of experimental characteristics associated to germination and seedling growth, and Gibberellic acid treatments were 0 mgL-1, 300 mgL-1, 600 mgL-1, and 900 mgL-1. Results: The effects of D-mannitol were significant in germination percentage, germination rate, seedling vigor index, chlorophyll a, total chlorophyll, leaf dry weight and total sugar. Experimental treatments such as germination percentage, germination rate, and total chlorophyll were also influenced by the application of gibberellic acid. The interaction between D-mannitol and gibberellic acid had significant effect on peroxidase activity. The highest germination percentage, germination rate, seedling vigor index, catalase activity, peroxide activity, and Chlorophyll a were obtained for 0 MPa application of D-Mannitol, followed by usage of 40 MPa, and 80 Mpa, respectively. The maximum total chlorophyll, relative water content, and leaf dry weight were also related to control treatment (0 MPa D-Mannitol), while the higher values of proline content, carotenoids and total sugar were obtained for 80 MPa application of D-Mannitol. The maximum values of germination rate, germination percentage, seedling vigor index, peroxidase activity, and chlorophyll a were related to control treatment (0 mgL-1 gibberellic acid), and the highest CAT activity was related to the application of 300 mgL-1 gibberellic acid. The highest total chlorophyll content and relative water content were related to 0 mgL-1 gibberellic acid, while the maximum praline content and leaf dry weight were obtained for the application of 600 mgL-1 and 0 mgL-1 gibberellic acid. There was not any significant difference in carotenoids between gibberellic acid treatments, while the application of 900 mgL-1 gibberellic acid obtained the highest value of total sugar of barberry. Conclusion: Conclusively, on the basis of the results, the application of 80 MPa D-Mannitol and 600 mgL-1 could be recommended as they can increase the absorption of nutrients and water.

Sulfuric acid as a germination stimulator in forage soybean seeds (Neonotonia wightii)

Forage soybean (Neonotonia wightii) is a legume frequently used as a cover crop in field crops. However, as a species of the Phaseoloideae subfamily, it is characterized by the physical latency of the seed, which makes it difficult to obtain a high, homogeneous, and rapid germination. The aim of the present study was to determine which method of scarification stimulates the germination process to a greater extent. The following immersion times were evaluated: 5 to 20 minutes in 98% sulfuric acid, 24 hours in 5.4% sodium hypochlorite, water, and gibberellic acid [100 ppm], 15 minutes of treatment with sandpaper and the combination of these last two. They were distributed in a completely randomized experimental design, where eight treatments and a control were evaluated, with three replicates each and 200 seeds as experimental units, subjected to controlled conditions (germination chambers at constant temperatures and a relative humidity of 27 °C and 70%, respectively) during the spring of 2022. The results were favorable, a high germination percentage was obtained from seeds subjected to a 20-minute immersion time in sulfuric acid (90% germination and an average of six germinated seeds per day) and this was significantly higher than the rest, making it an effective method to break the physical latency of the seeds, as it removes the impermeable cover allowing inhibition and consequently the activation of the seed’s metabolism.

Differential effects of increasing temperature on the germination of five wild species with varying range sizes in a Carrara marble quarry

Assessing the performance of spontaneous plants in abandoned quarries under future environmental scenarios is important for successful restoration practises. Air warming is one of the most relevant ongoing climatic changes in the Mediterranean. We tested the effects of increasing temperature on the germination of five species spontaneously colonising the abandoned sectors of a Carrara marble quarry (Tuscany, central Italy). We selected five plant species with different widths of their distribution range, from local endemic to invasive alien: Santolina pinnata, Globularia incanescens, Hypericum coris, Helichrysum italicum, and Buddleja davidii. Seeds were collected in situ and their germination was tested in laboratory conditions at 10, 15, 20, 25, and 30 °C. The effects of temperature and species on germination percentage (GP) and mean germination time (MGT) were tested through two-way ANOVA. Increasing temperatures negatively affected the GP of S. pinnata and H. coris. G. incanescens performed better at intermediate temperatures, whilst H. italicum benefited from increasing temperatures. B. davidii showed a similar high GP under all the treatments. MGT decreased with increasing temperature for all the species, except for S. pinnata, which showed an increase of MGT with increasing temperature. We highlighted that, under future warmer climatic conditions, the two endemic species will be disadvantaged with respect to the species with a wider distribution range and the non-native species in the studied quarry. This evidence is relevant for restoration planning since seeding the two endemic species could be unsuccessful under future environmental scenarios, when H. italicum and B. davidii will be more competitive in the colonisation of the quarry.

Response of Soil, Growth, and Biomass Properties of Lupin (Lupinus albus L.) to Wood ash and Sawdust

Two organic carbon resources (wood ash and sawdust) were applied in the study site to assess their profound effects on soil properties and growth and biomass properties of lupin (Lupinus albus L.). Because of their contribution to improving soil structure, enhanced water-holding capacity, and increased carbon sequestration. The experiments were conducted in mid-November 2022 at Grdarasha Research Station, College of Agricultural Engineering Sciences, Salahaddin University-Erbil, Iraq. The experiment includes two organic amendments (wood ash and sawdust) as a treatment and Control. Results showed that the application of wood ash and sawdust improved and raised the number of essential elements and heavy metals in the soil, which in turn boosted the ability of the roots to absorb nutrients. The highest germination percentage, plant height, and leaf number were observed in the ash treatment (95.00 %, 22.00 cm, and 21.22) respectively. The great values of fresh and dry shoot weight were found in sawdust treatment 63.78 and 14 g/plant) respectively. The effects of treatments were not significant on root length and fresh and dry root weights. The final findings showed that adding various forms of organic carbon could alter the chemical characteristics of the soil. This, in turn, might have a good impact on microbes, which can subsequently help plants grow by converting, solubilizing, and mobilizing soil nutrients. Furthermore, lupin plants appeared to be a form of phytoremediation, or absorption, for certain heavy metals.

Preparation, Characterization, and Testing of Compost Tea Derived from Seaweed and Fish Residues

Non-aerated compost tea (CT) was prepared from compost derived from rockweed (Ascophyllum nodosum) and fish (cod, common ling, haddock, saithe) residues that fermented in water. Electrical conductivity, pH, concentrations of dry matter, ash, C, macronutrients (N, P, K, Ca, and Mg), and micronutrients (Cu, Fe, Mn, Mo, and Zn) of CT prepared under different fermentation conditions were measured. The effects of process factors, i.e., water/compost mass ratio (4.2–9.8 g/g) and fermentation time (4.2–9.8 days = 100–236 h), on the physicochemical properties of CT were quantified using quadratic polynomial models. CT obtained at optimal levels of process factors (4.2 g/g and 5.6 days = 134 h) was tested for lettuce seed germination and seedling growth. Diluted CT (25% CT + 75% ultrapure water) improved seedling growth while achieving a high germination percentage (97%).

In vitro germination and seedling formation of Plantago tomentosa Lam. (Plantaginaceae): influence of concentrations of the MS medium

Plantago tomentosa Lam. (Plantaginaceae) is an herbaceous plant native to Brazil. It is widely used in folk medicine. The species has potential for use in the pharmaceutical and food industry due to its possible bioactive properties and the presence of mucilage in the seeds. The objective of this work was to investigate the influence of different concentrations of Murashige-Skoog (MS) medium on germination and seedling formation in vitro of P. tomentosa. The seeds were collected in Lajeado, disinfected, and inoculated in bottles with MS medium added to 7 g L-1 of agar and 30 g L-1 of sucrose, in four treatments: 25%, 50%, 75%, and 100% of the medium concentration. The experimental design was completely randomized, consisting of 17 replications of ten seeds for each treatment. The evaluation of germination and formed seeds was carried out every two days to determine the variables of germination percentage (GP), mean germination time (MGT), germination speed index (GSI), seedling formation percentage (SFP), mean seedling formation time (MSFT) and seedling formation speed index (SFSI). No significant difference was found between treatments for all the assessed variables. It is concluded that the use of a concentration of 25% of the MS medium is viable, as it promoted a high percentage of germination and seedling formation and in a time interval similar to that of the other concentrations, with the advantage of using fewer reagents.

Biomass of Eichhornia crassipes as an Alternative Substrate for the Formation of Lettuce Seedlings

The production of lettuce has increased significantly due to the use of hydroponic systems that rely on substrates. Disposal and acquisition costs present problems, necessitating the identification of sustainable alternatives. The present study aimed to evaluate the use of Eichhornia crassipes (water hyacinth) dry matter in a substrate for the formation of lettuce seedlings. Water plants were collected to obtain their dry matter, and twelve mixtures were formed with Sphagnum and perlite. Mixtures with more water hyacinth dry matter exhibited greater water retention. However, these mixtures also lost water at a faster rate than those containing primarily Sphagnum dry matter did. Higher percentages of germination were detected in the mixtures with water hyacinth dry matter, but these seedlings also presented higher concentrations of proline, such as 16.0 µg mL−1. The mixtures with water hyacinth dry matter presented the highest ion concentrations, mainly at high levels of humidity. Mixtures with a high proportion of water hyacinth dry matter had a greater water retention capacity and a high percentage of lettuce seed that germinated. The mixtures with a higher proportion of Sphagnum led to greater root length, greater concentrations of chlorophyll in cotyledonary leaves, and better morphological development of the seedlings.

Physiological Maturity and Harvesting Date on Seed Yield and Quality of Indigo (Indigofera suffruticosa Mill.)

Background: Indigo is used to dye many fabrics, which also needs a lot of indigo plants enough to dye the fabric. Indigo seeds were used for propagation, but the seed of indigo is not simultaneous. The physiological maturity and optimum harvesting date affected the yield and quality of indigo seeds. This research aims to study the physiological maturity and harvesting date of indigo on seed yield and quality. Methods: Indigo plants were grown in the dry season. The experiment was a randomized complete block design (RCBD) with 4 replications, consisting of 4 harvesting dates; 140, 150, 160 and 170 days after planting (DAP). Result: The results showed that the first indigo flower was at 51 DAP and matured to pods at 59 DAP. It takes 113 DAP for the indigo color to change from green to brown. The appearance of the pods changed from green to yellowish green, brown and black, respectively. As the indigo pods became 50% brown, they started harvesting. The weights of a dry pod, a fresh pod and a seed were different. When harvesting at 140 DAP, the fresh pod weight had the maximum of 82.60 g/plant and dropped as the indigo harvesting date grew (P less than 0.01). The dry pod weight was the lowest, at 27.58 g/plant (P less than 0.05). The seed weight per plant for harvesting at 170 DAP was 25.57 g. The harvesting date, however, did not differ statistically. The highest percentage of germination and vigor were from the indigo seeds, which were harvested at 140 DAP, 31.94% and 38.81%, respectively (P less than 0.01). Therefore, the optimum harvesting date at 140 DAP is the physiological maturity stage and harvesting of the pods when they turn brown for the highest seed quality.

Evaluation of endophytic colonization and establishment of entomopathogenic fungi against Tuta absoluta (Lepidoptera: Gelechiidae) in tomato plants.

The tomato, Solanum lycopersicum L., is one of the most important horticultural crops that can be consumed fresh or after being processed worldwide. The tomato leaf miner (Tuta absoluta) is one of the most devastating pest to tomato plants due to its mine-feeding nature in the mesophyll tissue of the plant. Fungal entomopathogens can exist naturally in plants as an asymptote. This study aimed to detect the endophytic colonization of Beauveria bassiana and Metarhizium robertsii within tomato plants via artificial inoculation and their virulence effects on Tuta absoluta. Isolates with the highest percent germination and virulence against T. absoluta were selected for endophytic evaluation within tomato plants by different artificial inoculation techniques. This study revealed that, isolates with the highest percent germination and virulent to Tuta absoluta had the potential to colonize tomato plants. The result showed that, the maximum mortality rate (97.5%) of Tuta absoluta larvae was achieved by Metarhizium robertsii isolate K-61 at a concentration of 1x10 8conidial/ml at 7 days post inoculated. However, the highest cumulative mortality (100%) was recorded by Beauveria bassiana isolate APPRC-27 at 10 days post inoculated through the direct contact method. The highest endophytic colonization was registered by isolate APPRC-27 (76.67%) at 7 days post-inoculated using the leaf spray technique, but it declined to 11.67% after 28 days of inoculated. In the case of the seedling inoculation technique, the highest endophytic colonization was obtained in the root tissues of tomatoes at 28 days of inoculated by isolate K-61. This study revealed that the leaf spray inoculation technique was the most effective method, followed by seedling inoculation, for the deployment of Beauveria bassiana and Metarhizium robertsii endophytes in tomato plant tissues. Therefore, virulent Beauveria bassiana and Metarhizium robertsii, are promising bioagents for the control of Tuta absoluta if deployed as endophytes.

INTRODUCTION TO IN VITRO CULTURE OF RHAPОNTICUM CARTHAMOIDES

The article presents the results of studies on the introduction into in vitro culture of the valuable medicinal plant Rhaponticum carthamoides, growing in the territory of the Kazakhstan Altai. R. carthamoides - or maral root is a source of various biologically active compounds (flavonoids, polysaccharides, saponins, cumanins). This species is of particular value as a producer of ultra-high quantities of ecdysteroids, which have a hepatoprotective and hypoglycemic effect, acting as adaptogens. Currently, natural reserves of this species are sharply reduced due to increasing demand and, as a consequence, lead to the depletion of natural populations. Modern biotechnology methods make it possible to develop highly effective approaches for the propagation and preservation of R. carthamoides for subsequent introduction and expansion of the range of fodder and medicinal crops that meet the needs of agriculture, medicine and the food industry. Seeds collected from their natural habitats were used as research material. A seed sterilization protocol has been developed; it has been established that to obtain aseptic seedlings it is necessary to use a multi-stage sterilization protocol using various sterilizing agents. Surface-sterilized seeds were placed on MS and MS media containing ½ mineral salts with different hormonal compositions. The results showed that the percentage of seed germination varied depending on the hormonal status of the environment and ranged from 42.9 to 85.5%. Thus, the lowest percentage of germination was noted on the B0 medium option (½ MS) - 42.9%. The highest percentage of germination was in seeds on the B3 medium option (MS + 3 mg/l BAP + 3 mg/l kinetin) - 85.5%.

Reproductive biology of Santolina ligustica Arrigoni, a rare narrow-ranged species: implications for conservation

Abstract Santolina ligustica is a rare narrow-ranged species endemic to Liguria (Italy) for which a likely trend of decline has been recorded. For this reason, it would be important to assess the reproductive biology of this endemic species. In this study, we investigated the plant–pollinator interactions, the reproductive mode of the plant, and we evaluated both reproductive efficiency and seeds’ germination performances. Santolina ligustica is an obligate cross-pollinating species with a generalist pollination system. The most frequent insects are Coleoptera belonging to the genus Oedemera. Seed set is low, but the seeds germinate under a wide range of temperatures. Taken together, our results indicate that S. ligustica is an insect pollinated and self-incompatible plant, in which low visitation rate, secondary pollen presentation and/or lack of compatible mate may cause a low seed set per capitulum. Nevertheless, the high number of capitula per plant and the high percentage of germination may assure high number of seedlings. Thus, the survival of S. ligustica is probably threatened by other factors such as fragmented distribution and habitat reduction because of land-use change. Under this scenario, we proved that species’ decline is not directly related to reproductive failure and different studies are desirable to provide suggestions for the species conservation.

Evaluation of different spring rice genotypes for seedling stage growth at Tulsipur, Dang, Nepal

Rice is the fundamental staple crop of Nepal. For food security, the production rate of main season rice is insufficient; nevertheless, spring rice can be a possibility. Spring rice is short duration crop as compared to the main season crop as, it can be best utilized through its cultivation. An experiment on spring rice (Oryza sativa) was performed to examine ten different elite rice genotypes in irrigated seedling stage at Campus of Live Sciences, Tulsipur, Dang from April to May 2023 with an objective to assess the response of different genotypes of seedling stage under controlled environment condition and to compare seedling stage growth and development of different spring rice variety The study was conducted with three replications on a completely randomized design (CRD). Data on the seedlings' growth was gathered at intervals of ten days. The outcome showed that there were statistical differences in morphological features between various genotypes. In comparison to other plants, IR09R270 had the highest average plant height (12.9 cm), IR17A106 had the greatest amount of leaves (2.44), IR09R270 had the greatest length of leaves (6.59 cm), Svin080 had the widest leaves (0.18 cm), IR112208B-B-RGA-BRGA had the longest shoots length (16.46 cm), and IR112208B-B-RGA-BRGA had the longest roots length (9.30 cm). The sample with the highest percentage of germination (85.65%) was IR112208B-B-RGA-BRGA. The majority of genotypes were found to have heritability more than 60%, indicating a greater contribution from genetic factors than environmental conditions. It was discovered that the phenotypic coefficient of variance (PCV) was marginally larger than the genotypic coefficient of variance (GCV), suggesting that the environment had little effect on how characters are expressed. The study's conclusions demonstrated that the rice genotypes IR09R270 and IR112208B-B-RGA-BRGA were preferable to other elite genotypes found in the Terai region. In conclusion under irrigated conditions, rice genotypes IR09R270 and IR112208B-B-RGA-BRGA performed well.