Higher Percentage Content Research Articles

Зміни жирнокислотного складу проростків кукурудзи за впливу мікробіологічного комплексу

Orientation of agricultural production to ecological expediency leads to interest in the use of microbial preparations that are able to increase the resistance of plants to negative environmental factors and activate germination processes. This study was conducted with the aim to investigate the influence of a microbiological complex on the sowing properties of seeds and the fatty acid composition of seedlings of different breeding samples of corn. Methods. Corn seeds of two lines 1-Ak026 and 2-Ak033 and their simple interline hybrid, which is the parent component of the three-line hybrid NUBiSe included in the State Register of plant varieties suitable for distribution in Ukraine, were used for the research. Seed inoculation was carried out with a microbial complex, which contained biological preparations Sporazyn, Avercom, and Phytovit. Seedlings from the seeds of the corresponding corn samples not treated with the microbial complex served as controls. The fatty acid composition of lipids was determined in the biological material of corn seedlings of different variants of the experiment. The Folch method was used to extract fats from plant material. Hydrolysis and methylation of fatty acids of lipids obtained from corn seedlings was carried out according to DSTU ISO 5509-2002. Fatty acids were detected and identified by gas chromatography. The quantity of fatty acids was calculated by the method of internal normalization in percentages. Results. The influence of the microbiological complex on the quantitative and qualitative composition of fatty acids of the lipid complex of corn seedlings was established, which depended on the breeding sample. In the process of research, 22 fatty acids were detected and quantitatively identified in corn seedlings. In the seedlings of lines 1-Ak033 and 2-Ak033, 20 and 19 of them were found, respectively, and in the hybrid – 18. The highest percentage content (more than 90 %) was revealed for palmitic (C16:0), oleic (C18:1n9c), and linoleic (C18:2ω6c) fatty acids. The use of the microbial complex made it possible to detect the fatty acids in line 1 and the hybrid that were not detected in the samples without inoculation. Conclusions. The study of the fatty acid composition of the microbial preparation Sporazyn revealed the highest content of unsaturated fatty acids (67.47 %) and the ratio of saturated to unsaturated (0.48). The use of microbiological preparations on corn seeds increased the activity of the germination processes, which led to the improvement of sowing properties and changes in the fatty acid composition of the lipid complex of seedlings of various breeding forms of Zea mays.

ON THE AGE AND CONDITIONS OF THE FORMATION OF THE POLYANYTSKA SUITE ACCORDING TO THE FORAMINIFERA STUDY RESULTS (LOWER MIOCENE, BORYSLAV-POKUTTYA NAPPE, UKRAINIAN CARPATHIANS)

Neogene deposits of the Polyanytska Suite were exposed by the South Boryslav-1 well within the eastern block of the South Boryslav structure of the Boryslav oil and gas condensate field of the Outer Carpathians. The studied sediments of the Polyanytska Suite belong to the complex zone of planktonic foraminifers Globigerina pseudoedita with a characteristic association: Globigerina pseudoedita, Globoquadrina dehiscens, Dentoglobigerina langhiana, Trilobatus trilobus, Tenuitella brevispira, Tenuitella denseconnexa, Globorotalia tetracamerata, Globigerinella evoluta, Globigerinella obesa, Chiloguembelina gracillima. The Globigerina pseudoedita zone of planktonic foraminifera correlates with the zonal interval of nannoplankton NN2-NN3 of the Central Paratethys, which covers the upper part Eggerian, Eggenburgian, and the lowest part Ottnangian of the Central Paratethys and is compared with the upper Aquitan – the lower part of the Burdigal of the lower Miocene of the Neogene of the International Chronostratigraphic Scale. The sediments of the Polyanytska Suite were formed in a normally saline sea basin, with depths from the middle to the inner shelf. The medium shelf conditions (intervals 1812–1805 m; 1684–1645 m and 1546–1485 m in the the South Boryslav-1 well) are indicated by a relatively high percentage content and morphology of the shells of planktonic foraminifera, the taxonomic composition of calcareous benthic foraminifera. The presence of plankton indicates normal water salinity. Analysis of foraminifera in the interval 1395–1365 m indicates the depths of the inner shelf. The identified redeposited foraminifera of the Paleocene-Lower Oligocene testify to the erosion and demolition of clastic material from the uplifted part of the Carpathians.

Testing and Evaluation Method Investigates Cement Expansion in Cased-Hole Wells

This article, written by JPT Technology Editor Chris Carpenter, contains highlights of paper SPE 208679, “Cement Expansion in Cased-Hole Environments: A Novel Laboratory Testing and Evaluation Method With Successful Field Implementation,” by Vasilii Sukhachev, Amir Salehpour, SPE, and Ilshat Akhmetzianov, SPE, Schlumberger, et al. The paper has not been peer reviewed. Preventing bulk shrinkage of cement in cased-hole wells is important when dealing with the risk of sustained casing pressure (SCP). Current studies based on membrane tests suggest that, even with a high-percentage content of expanding agents, cement systems show little or no expansion in dry media. An alternative laboratory method for measuring cement expansion, followed by a successful field application, was used to evaluate the total bulk volume change of the cement based on curing in a dry environment and in water. Optimal cement-slurry properties were obtained. The developed cement system, which was successfully implemented in the field, provided long-term zonal isolation with no indication of SCP to date. Introduction For a major operator in the Caspian region, drilling development wells required passing through shallow overpressured permeable zones containing water and biogenic gas. The optimal casing design used to minimize the likelihood and severity of SCP consisted of isolating the shallow-soil zone by use of a cemented 24-in. liner followed by a 20-in. surface casing cemented using a two-stage method (Fig. 1). During the first stage, the cement was pumped inside of the 24-in. liner. Then, an external casing packer placed in the 20-in. casing was inflated against the 24-in. liner before performing the second-stage cement job. The second-stage cementing operation brought the cement above the top of 24-in. liner and provided sufficient length to isolate against the shallowest distinct permeable zone. Cement is considered a primary wellbore barrier element. However, the cement placed between the 24-in. liner and the 20-in. casing and in the annulus of the 30-in. conductor and 20-in. casing is subject to shrinkage. This shrinkage leaves a potential leak path all the way to the wellhead on an offshore platform. The cement systems chosen consisted of the 1.92-SG (16-lbm/gal) slurry for the second stage cementing of the 20-in. casing because this stage was the system most susceptible to shrinkage. This slurry was placed in the annular space of the 20-in. casing and both the 24-in. liner and the 30-in. conductor. The curing temperatures and pressures in the field are 32°C and 6.74 MPa at the bottom of the well and 28°C and 2.48 MPa at the top of the cement. The top of the cement will be in direct contact with the spacer, a fluid with 89% water content; however, the remainder of the cement column will not have access to excess water during curing. If acceptable results could be achieved with the 1.92-SG cement system, then the same blend could be used for the 24-in. liner tail and the first-stage tail slurry of the 20-in. casing. This method is a possible solution to obtain a more-robust system that prevents the risk of SCP.

STUDY OF LIPIDS AND SOME BIOLOGICALLY ACTIVE COMPOUNDS OF THE FLOWERS SAMBUCUS NIGRA GROWING IN GEORGIA

The goal of the research was to study flowers of Sambucus nigra L. for the content of lipids and some biologically active compounds. There was obtained sums of neutral and polar lipids from the investigated object with various percentage yield. There was established the basic classes of lipids in them, was determined some physical – chemical constants of them. By using the gas-chromatographic method analysis in the sum of neutral lipids quantitatively and qualitatively were identified saturated, unsaturated and poly-unsaturated fatty acids with the high percentage content some of them. In the sum of polar lipids qualitatively and quantitatively is established and determined phospholipids. On the basis of research is established some other biologically active compounds in the mentioned object – carotenoids and aminoacids. On the basis of received results the vegetable oil from the flowers of Sambucus nigra L. is rich in biologically active compounds, which gives the opportunity to use it in medical practice.

A Comparison of Characteristics of Periodic Surface Micro/Nano Structures Generated Via Single Laser Beam Direct Writing and Particle Lens Array Parallel Beam Processing

Abstract Changing material surface micro/nanostructures using laser beam texturing is a valuable approach in wide applications such as control of cell/bacterial adhesion and proliferation, solar cells and optical metamaterials. Here, we report a comparison of the characteristics of surface micro/nanostructures produced using single beam laser direct writing and particle lens array parallel laser beam patterning. A Nd:YVO4 nanosecond pulsed laser at the wavelength of 532 nm was used in the laser direct writing method to texture the stainless steel surface submerged in water and in air with different scanning patterns. Changes in surface morphology, wettability, surface chemistry, and optical reflectivity were analyzed. In the particle lens array method, an excimer nanosecond laser at 248 nm wavelength was adopted to produce surface patterns on GeSbTe (GST) film coated on a polycarbonate substrate by splitting and focusing a single laser beam into millions of parallel breams. Single beam laser direct writing shows that the surface of high roughness and oxygen percentage content presented high wettability and low reflectivity characteristics. However, the controllability of the type of surface micro/nanopatterns is limited. The parallel laser beam processing using particle lens array allows rapid production of user designed periodic surface patterns at nanoscale overcoming the optical diffraction limit with a high degree of controllability. Controlling the uniformity of the particle lens array is a challenge.

Effects of Hydrophobicity on Antimicrobial Activity, Selectivity, and Functional Mechanism of Guanidinium-Functionalized Polymers.

In this study, a series of guanidinium-functionalized polycarbonate random co-polymers is prepared from organocatalytic ring-opening polymerization to investigate the effect of the hydrophobic side chain (ethyl, propyl, isopropyl, benzyl, and hexyl) on their antimicrobial activity and selectivity. Although the polymers exhibit similar minimum inhibitory concentrations, the more hydrophobic polymers exhibit a faster rate of bacteria elimination. At higher percentage content (20 mol%), polymers with more hydrophobic side chains suffer from poor selectivity due to their high hemolytic activity. The highly hydrophobic co-polymer, containing the hydrophobic hexyl-functionalized cyclic carbonate, kills bacteria via a membrane-disruptive mechanism. Micelle formation leads to a lower extent of membrane disruption. This study unravels the effects of hydrophobic side chains on the activities of the polymers and their killing mechanism, providing insights into the design of new antimicrobial polymers.

Characterisation and Comparison of Pith and Cortex of Napier Grass Stem

In the previous study, only pith part had been attracted to be investigated by researcher eventhough cortex had a high percentage content in the Napier grass stems (NG). Hence, further studied can help to achieve the aim of maximizing the use of NG stems by using cortex and pith in manufacturing biopolymers. For this purpose, the thermal,chemical, morphological characterization of cortex and pith of NG stems had been explore. The results of TGA, FTIR, microscope, and SEM had shown the difference in the cortex and pith of NG stems. Although their peak absorption value appear to be different, the group involved in pith still present in the cortex. The cortex showed a high decomposed percentage which was 93.79 % compared to the pith with 92.55 %. The micrographs images display the presence of hemicellulose, lignin, impurities, and wax on the surface of cortex and pith. Overall, the results of the cortex showed that it had the potential to be used the same as the pith for the production of biopolymers in the future.

The Influence of Primary Electrons on the Mechanism of Plasma Generation

It is shown that at high percentage content of primary electrons, plasma is generated due to the retention of ions in a potential well created by electrons and not due to the accumulation of secondary electrons in the region of a potential hump formed by ions.

Effect of urea, compost, manure and bio-fertilizers on yield, percentage and composition of fatty acids of sesame seed oil (Sesamum indicum L.)

This study was carried out in Iran during two growing seasons 2016-2017. Sesame is one of the earliest oilseed plants, high oil percentage and high content of unsaturated fatty acids, especially oleic acid and linoleic acid, have led to an increase in the nutritional quality of sesame oil. Accordingly, a two-year experiment was conducted as factorial based on randomized complete block design with three replications. The evaluated factors included four levels of urea as nitrogen fertilizer (0, 33, 66 and 100% of plant requirement), three kinds of organic fertilizer (manure, vermicompost and control) and four kinds of biofertilizers (Azosperillum, Azotobacter, Azosperillum +Azotobacter and control). At the end of the growing season, seed yield, percentage and yield of seed oil and fatty acids composition (oleic acid, linolenic acid, linoleic acid, stearic acid and palmitic acid) were measured. The results showed that the effect of different levels of nitrogen, organic and biological fertilizers on all studied traits was significant in two years of experiment and in all three environments (control, manure and compost). Vermicompost fertilizer and Azosperillum + Azotobacter have a more favorable effect on traits than other levels of each of these fertilizers, but nitrogen fertilizer levels have different effects on different traits. The highest seed yield (1298 kg ha-1) was obtained for control environment in 100% nitrogen application, for manure environment in 33% nitrogen application (1565 kg ha-1) and for compost environment in 66% nitrogen application (1799 kg ha-1).

Heterogeneous activation of peroxymonosulfate by different ferromanganese oxides for tetracycline degradation: Structure dependence and catalytic mechanism

In this work, ferromanganese oxides (FMOs) synthesized with different Fe and Mn ratio using sol–gel method showed good activity in the activation of peroxymonosulfate (PMS) for tetracycline degradation. The structure and surface characterizations of the FMOs were measured using XRD, BET and XPS. The variation of the catalytic performance of FMOs in the degradation of TC was investigated with varied initial conditions. The FMO-46 structured as FeMnO3 with cubic structure could activate PMS efficiently due to the large surface area and surface Mn(III) sites. While the high catalytic activity of FMO-73 was attributed to the high percentage content of surface Fe(II) and Mn(II). The more vicinities of Fe and Mn exist on the surface of FMOs result in a better catalytic activity towards PMS and degradation of TC. The catalytic mechanism was elucidated by detecting the reactive oxidation species generated using ESR measurement and radical scavengers. It was found that Mn-oxides with Fe-regulated-surface active sites were expected to enhance the catalytic activity in the generation of SO4−. The experimental results demonstrated that SO4− had a higher contribution to degrade tetracycline in PMS/FMO system than OH. This study proves that surface regulated FMOs can efficiently activate PMS to degrade refractory organic pollutants as an environmentally friendly catalyst.

Effect of storage conditions, packing materials and seed treatments on viability and seedling vigour of onion (Allium cepa l.) seeds

The present investigation was carried out from July 2013 to July 2015, wherein 100 g of fresh quality seed of onion cv. GWO 1 was having high germination percentage and moisture content below 8 per cent. The treatment consisted of two storage conditions (C) viz., C1 (Ambient temperature) and C2 (Cold storage at 70C + 20C); two packing materials (P) viz., P1 = Cloth Bag and P2 = Polythene Bag (500 gauge = 125 µ), and five seed treatments (S) viz., S1 = Control, S2 = Carbendazim @ 2g/kg seed, S3 = Mancozeb @ 2g/kg seed, S4 = Thirum @ 3g/kg seed, and S5 = Neem leaf powder @ 10g / kg seed. After proper mixing or smearing the seeds as per the treatments, seeds were packed and stored as per treatments. Observations were recorded at 90 days interval on viability and vigour parameters. The results revealed that seed stored under cold storage (7±2 °C) and in polyethylene bags (500 gauge) noted significantly higher values for all the characters even after two years of storage. All the treatment combinations of seed stored under cold storage gave more than 70 per cent germination (As per ISTA standard) even after two years of storage, of which, seed treated with thirum @ 3g/kg seed was the best treatment. Therefore, it can be concluded that seed of onion can be stored up to two year in cold storage packed in polyethylene bag without or with seed treatment without deterioration in germination and seedling vigour.

Effect of different storage conditions and seed treatments on seed viability in soybean [Glycine max (L.) Merr.

The present investigation was carried out in laboratory of the Department of Seed Science and Technolo-gy, College of Agriculture, Junagadh Agricultural University, Junagadh from the April 2013 to April 2015, wherein two kg of freshly harvested quality seed of soybean cv. Gujarat Junagadh Soybean 3 having high germination percentage and low moisture content (below 8%) was taken for each repetition and for each combination of treat-ments. The treatment consisted of two storage conditions (C) viz., C1 (Ambient temperature) and C2 (Cold storage at 7oC + 2oC), and five seed treatments (S) viz., S1 = Control, S2 = Carbendazim @ 2g/kg seed, S3 = Mancozeb @ 2g/ kg seed, S4 = Neem leaf powder @ 10g/kg seed, and S5 = Neem Oil @ 5 ml/kg seed. The experiment was carried out using Completely Randomized Design (Factorial) repeated three times. After proper mixing or smearing the seeds as per the treatments, seeds were packed in cloth bag and kept in laboratory under two different storage conditions. Observations were recorded at 90 days interval on germination (%), root length (cm), shoot length (cm), seedling dry weight (g), seed vigour index I, seed vigour index II and seed moisture content (%). The results revealed that storage condition (C) and seed treatments (S) exhibited significant differences almost for the all the traits for germination and seedling parameters after 2 years of storage. The results of soybean seed stored in two different storage conditions showed that on an average, the seed stored under cold storage (70C + 20C) noted higher values for all the traits studied except seed moisture content after 2 years of storage. Among the seed treatments, on an average, after 2 years of seed storage, significantly (P<0.05) higher values were recorded by all the seed treatments over the control. However, seed treated with Mancozeb @ 2g/kg of seed recorded the significantly highest germination percentage (71.50 %) and it was at par with Neem leaf powder @ 10 g / kg seed (70.67%) and Carbendazim @ 2g /kg seed (69.67%) after 2 years of storage. The germination percentage noted in control treatment was 33.17 per cent after 2 years of storage. An ISTA standard for germination in soybean is 70 per cent. Most of the interactions effects were found significant (P<0.05) for all the traits studied.

Performance of neem products on the storability of mungbean[Vigna radiata (L.) Wilczek] seeds

The present investigation was carried out in laboratory of the Department of Seed Science and Technology, College of Agriculture, Junagadh Agricultural University, Junagadh from August, 2013 to June, 2015, wherein two kg of freshly harvested quality seed of mungbean cv. GM 4 having high germination percentage and low moisture content (below 8%) was taken for each repetition and given the treatments. The experiment was carried out using completely randomized design (CRD). After proper mixing or smearing, seeds were packed in 500 gauge polythene bags and kept in laboratory under ambient condition. Observations were recorded at bimonthly interval on germination (%), root length (cm), shoot length (cm), seedling dry weight (g), seed vigour index I, seed vigour index II and seed moisture content (%). The results indicated that all the treatments exhibited significant difference for all the traits studied except shoot length after two years of storage. The Indian Minimum Seed Certification Standards in mungbean for germination percentage is 75 per cent. Looking to the germination percentage and Seed vigourindex I and II, among all the treatments, T1 (Neem Seed kernel powder @ 5 g/kg seed), T2 (Neem seed kernel powder @ 10 g/kg seed), T3 (Neem Cake @ 5g/kg Seed) and T4 (Neem Cake @ 10g/kg Seed) recorded the higher values for these traits and can be recommended as a seed treatment before storage for maintaining the seed germination and seedling vigour for more than two years.

The application of mycelial wastes from microbiological production of antibacterial antibiotics as lubricating oil additives

The antiwear and antitear properties of mycelial wastes from production of antibacterial antibiotics have been studied. The waste mycelium from fucidin production has been shown to exhibit the best antiwear and antitear performance due to a higher percentage content of phosphorus and sulfur as compared to the mycelial wastes from production of other antibacterial antibiotics.

Investigation of CYP3A4 and CYP2D6 Interactions of Withania somnifera and Centella asiatica in Human Liver Microsomes.

Withania somnifera is commonly used as a rejuvenator, whereas Centella asiatica is well known for its anxiolytic and nootropic effects. The present study aims at investigating the effect of crude extracts and principal phytoconstituents of both the medicinal plants with CYP3A4 and CYP2D6 enzyme activity in human liver microsomes (HLM). Phytoconstituents were quantified in the crude extracts of both the medicinal plants using reverse phase HPLC. Crude extracts and phytoconstituents of W. somnifera showed no significant interaction with both CYP3A4 and CYP2D6 enzymes in HLM. Of the crude extracts of C. asiatica screened in vitro, methanolic extract showed potent noncompetitive inhibition of only CYP3A4 enzyme (Ki-64.36 ± 1.82 µg/mL), whereas ethanol solution extract showed potent noncompetitive inhibition of only CYP2D6 enzyme (Ki-36.3 ± 0.44 µg/mL). The flavonoids, quercetin, and kaempferol showed potent (IC50 values less than 100 μM) inhibition of CYP3A4 activity, whereas quercetin alone showed potent inhibition of CYP2D6 activity in HLM. Because methanolic extract of C. asiatica showed a relatively high percentage content of quercetin and kaempferol than ethanol solution extract, the inhibitory effect of methanolic extract on CYP3A4 enzyme activity could be attributed to the flavonoids. Thus, co-administration of the alcoholic extracts of C. asiatica with drugs that are substrates of CYP3A4 and CYP2D6 enzymes may lead to undesirable herb-drug interactions in humans.

The Interface Morphology of Thixo-Joined Cold Work Tool Steel

Cold-work tool steel is considered to be a nonweldable metal due to its high percentage content of carbon and alloying elements. The application of a new process of the semi-solid joining of two parts of AISI D2 cold-work tool steel is proposed using a partial remelting method. Samples were heated in an argon atmosphere up to 1275°C which corresponded to about 20% of liquid fraction and held for 10 minutes. Metallographic analyses along the joint interface showed a smooth transition from one to the other and neither oxides nor micro-cracking was observed. The current work successfully confirmed that avoidance of a dendritic microstructure in the semi-solid joined zone and high bonding quality components can be achieved without the need for force or complex equipment when compared to conventional welding processes.

Semi-Solid Joining of D2 Cold-Work Tool Steel

Cold-work tool steel is considered to be a non-weldable metal due to its high percentage content of carbon and alloying elements. To address this problem the application of a new process of semisolid joining using a direct partial remelting method was developedto achieve a spherical join structure between two parts of AISI D2 cold-work tool steel. Since the surface oxidation of this metalis very high, the control of the atmosphere during joining had to be considered. Samples were heated in an argon atmosphere at two different temperatures of 1250°C and 1275°C for 10 minutes. Metallographic analyses along the joint interface showed that an increase in temperature promoted the final joining properties and also that at a liquid fraction of 15% joining was not fully practicable. However, a20% liquid fraction can produce a very good joint and microstructure as compared to the other experimental liquid fraction. Metallographic analyses along the joint interface showed a smooth transition from one to the other and neither oxides nor microcracking was observed. The current work confirmed that avoidance of a dendritic microstructure in the semisolid joined zone and high bonding quality components can be achieved without the need for force or complex equipment when compared to conventional welding processes.

Modified Wallschläger Sequential Extraction as a Tool for Evaluating the Mobility of Mercury

Abstract The presented studies were focused on evaluating the utility of one of sequential extraction methods for evaluating the bioavailability of mercury in soils polluted by this element. Soil samples collected from horizons 0-20 cm and 20-80 cm were subject to analysis of the basic physical and chemical properties of soils. Moreover, the total content of mercury was determined and sequential extraction of mercury was conducted using a modified five-stage Wallschläger method. The analyses show that the studied soils are characterized by a variable mercury content, the highest in the surface soil horizons. Sequential extraction of mercury in the analyzed soils has indicated that the highest percentage content in the total content had mercury linked with sulphides. A high content of mercury linked with organic matter was also noted. The content of bioavailable mercury did not exceed 1.5% of the total content.

Influence of physical seed enhancement techniques on storability of groundnut Kernals (Arachis hypogaeaL.) cv. VRI 2

Genetically pure seeds of groundnut cv. VRI 2 were obtained from the Oilseed Research Station, Virudhachalam. The bulk seeds were graded for uniformity using 18/64 size sieve and were imposed with various physical seed enhancement techniques. The treated seeds along with control were stored in cloth bag and 700 gauge polythene bag under ambient condition of Annamalai nagar for a period of 10 months and were evaluated for seed quality characters viz., moisture content, germination per cent, oil content and electrical conductivity at bimonthly intervals. The study revealed that the seeds treated with halogen slurry treatment and stored in 700 gauge polythene bag registered low moisture content (8 %), electrical conductivity (0.529 dsm-1), high germination percentage (74 %) and oil content (43 %) when compared to control. It was also noted that they maintained the minimum seed certification standard upto 10 months of storage.

Exploitation of Rape Flow by Bee Colonies in Stationary and Migratory Apiary

Summary The aim of the study conducted in 2009 - 2011 was to estimate the differences in the nectar and pollen oilseed rape flow exploitation by bee colonies kept in the stationary apiary (permanently located on the rape field) and in the migratory apiary. The migratory apiary was transferred to the rape field at the appropriate time and situated on the same area as the stationary apiary. Every study year, 8 bee colonies per apiary, in two apiaries of different types (stationary and migratory apiary), were prepared. The colonies from the migratory apiary were placed on the rape field when about 10% of rape flowers were blooming. During that time, bottom pollen traps were inserted into the hives of both apiaries. The pollen loads were collected every day, separately from each colony. After the end of the flow season, honey was extracted separately from each colony. The honey was weighed and samples were taken for the palynological analysis. The weather conditions were monitored during the whole study period. The average harvest of pollen loads from one bee colony during one day, was similar in both apiaries. The content of Brassica napus pollen was significantly higher in the pollen loads harvested from the migratory apiary. Despite the fact that the amounts of honey extracted from both apiaries were similar, the microscopic pollen analysis showed significantly higher percentage content of rape pollen in the honey from the migratory apiary. The results confirmed that placing the migratory apiary in the winter rape field at the time when about 10% of flowers were blooming resulted in a better exploitation of the rape flow by the bee colonies from this apiary in comparison to the colonies from the stationary apiary.