High Levels Of Oleic Acid Research Articles

The Nutritional Potential of Avocado By-Products: A Focus on Fatty Acid Content and Drying Processes.

The aim of this study was to analyze the content of fatty acids and tocopherols in various components (pulp, seeds, peel) of avocado (Persea americana), which are often neglected as by-products. In addition, the effects of different drying processes on these components were investigated and the health benefits of the main fatty acids contained in avocados were highlighted. The samples were subjected to three drying processes: hot air (HAD), vacuum (VD), and hot-air microwave (HAMD). In all parts of fresh avocado, oleic acid was the most abundant (41.28-57.93%), followed by palmitic acid (19.90-29.45%) and linoleic acid (8.44-14.95%). Drying led to a significant reduction in the oleic acid content, with palmitic acid showing the greatest stability. HAD resulted in higher levels of oleic acid and linoleic acid in dried pulp and peel samples compared with VD and HAMD, while HAMD had the highest content of α-linolenic acid in all parts. In addition, HAMD had the shortest drying time. HAMD duration was 35 min, which was 76.7% shorter than HAD (150 min) and 82.5% shorter than VD (200 min). Considering fatty acid retention and drying efficiency, HAMD appears to have been the most effective method, especially for the avocado peel. Remarkably, the avocado peel consistently contained higher total tocopherol, with δ-tocopherol generally being the most abundant form. The high content of tocopherols, oleic acid, and linoleic acid in the avocado peel suggests promising health benefits.

Effects of gender and slaughter age on physicochemical and quality traits of Korean Hanwoo striploin.

Hanwoo beef is in high demand because of its unique flavor, freshness, and high-fat content. However, the longer rearing period required to enhance marbling in Hanwoo cattle has adverse environmental consequences, such as greenhouse gas emissions and overall rearing costs. To address consumer preferences for leaner and healthier meat, the Korean meat industry has recently introduced Hanwoo heifer meat as an alternative source, but its quality traits are still unclear. Nevertheless, there is a limited body of research exploring the impact of Hanwoo gender (steer, heifer, and cow) and their corresponding slaughter ages on meat quality traits. This study looked into how gender affected the physicochemical and qualitative features of Hanwoo striploin at their respective slaughter ages. Results revealed that cow striploin has higher levels of moisture (66.81%) and protein (20.76%), whereas it contains lower levels of fat (10.66%) and cholesterol (34.66 mg/100 g). Regarding the physicochemical properties, cow striploin exhibited significantly lower shear force, color indexes, and soluble collagen (p < 0.05). However, chondroitin (1.19%) and muscle fiber area (1,545.23 μm2) were significantly higher in steer striploin than in heifer and cow (p < 0.05). Cow striploin exhibited significantly higher levels of oleic acid, unsaturated fatty acids (UFAs), and monounsaturated fatty acids (MUFAs) while having lower levels of eicosadienoic acid and atherogenic index compared to the other two groups. Cows and heifers had higher concentrations of amino acid metabolites than striploin from steers. Furthermore, bioactive metabolites such as carnitine and carnosine content were found higher in cow and heifer respectively. Overall, Hanwoo cattle gender influences the qualitative attributes of striploin; nevertheless, compared to steer and heifer striploin, cow striploin is a relatively good source of protein, fatty acid content, and metabolites conducive to a healthy diet.

Formulasi Sediaan Hair Tonic Ekstrak Rumput Laut (Hormophysa Triquetra) Dengan Kombinasi Ekstrak Kemiri (Aleurites Moluccana) Sebagai Penumbuh Rambut

Hormophysa triquetra seaweed is one of the marine natural resources that has not been utilized optimally by society. H. triquetra seaweed has a dominant pigment, namely fucoxanthin, which has anti-cancer and anti-obesity properties. Seaweed is widely used as a cosmetic product by utilizing its ingredients. H. triquetra seaweed contains flavonoids, phenols as antioxidants which are able to repair damaged hair cells and produce skin tissue which is conducive to hair growth, besides the vitamin C content which can increase blood circulation and provide nutrition for hair follicles. Aleurites moluccana candlenut oil contains high levels of oleic acid and has activity as a hair growth stimulant which has been widely used for generations as a hair tonic. In addition, the antioxidant properties of candlenut oil's oleic acid are believed to slow hair loss and accelerate hair growth. The aim of this research is to determine the formulation and characteristics of the best hair tonic made from H. triquetra seaweed and candlenut extract as a hair growth agent. A completely randomized design with 4 treatments and 3 replications was used in this experiment. The best results were in the F0 treatment because it showed good physical stability in the test animals and fast hair length growth among other treatments because the samples used contained the highest seaweed extract and contained antioxidants which could protect hair and maintain good hair pigmentation.

High-throughput diagnostic markers for foliar fungal disease resistance and high oleic acid content in groundnut

BackgroundFoliar diseases namely late leaf spot (LLS) and leaf rust (LR) reduce yield and deteriorate fodder quality in groundnut. Also the high oleic acid content has emerged as one of the most important traits for industries and consumers due to its increased shelf life and health benefits.ResultsGenetic mapping combined with pooled sequencing approaches identified candidate resistance genes (LLSR1 and LLSR2 for LLS and LR1 for LR) for both foliar fungal diseases. The LLS-A02 locus housed LLSR1 gene for LLS resistance, while, LLS-A03 housed LLSR2 and LR1 genes for LLS and LR resistance, respectively. A total of 49 KASPs markers were developed from the genomic regions of important disease resistance genes, such as NBS-LRR, purple acid phosphatase, pentatricopeptide repeat-containing protein, and serine/threonine-protein phosphatase. Among the 49 KASP markers, 41 KASPs were validated successfully on a validation panel of contrasting germplasm and breeding lines. Of the 41 validated KASPs, 39 KASPs were designed for rust and LLS resistance, while two KASPs were developed using fatty acid desaturase (FAD) genes to control high oleic acid levels. These validated KASP markers have been extensively used by various groundnut breeding programs across the world which led to development of thousands of advanced breeding lines and few of them also released for commercial cultivation.ConclusionIn this study, high-throughput and cost-effective KASP assays were developed, validated and successfully deployed to improve the resistance against foliar fungal diseases and oleic acid in groundnut. So far deployment of allele-specific and KASP diagnostic markers facilitated development and release of two rust- and LLS-resistant varieties and five high-oleic acid groundnut varieties in India. These validated markers provide opportunities for routine deployment in groundnut breeding programs.

The shift in the fatty acid composition of the circulating lipidome in Alzheimer's disease.

Fatty acids (FAs) are the building blocks of complex lipids and signaling compounds; the role of the lipidome fatty acid profile (LFA) in AD progression remains unclear. The LFA of plasma and cerebrospinal fluid (CSF) samples from 289 participants (103 AD patients, 92 MCI patients, and 94 controls) was determined by GC-FID. The MCI subjects were followed up for 58±12.5 months. In controls, CSF has a more neuroprotective LFA than plasma. In CSF, a higher content of docosahexaenoic acid was associated with a reduced risk of MCI-to-AD progression. In plasma, higher oleic acid content was associated with lower risk of AD, MCI, and MCI-to-AD progression, whereas higher levels of vaccenic acid and docosahexaenoic acid were associated with greater risk of AD and MCI, and higher rate of MCI-to-AD progression, respectively. The circulating LFA is involved in the pathogenesis and progression of AD. The lipidome fatty acid profile in CSF and plasma was markedly different. Higher levels of vaccenic acid and lower levels of oleic acid in plasma were associated with greater risk of Alzheimer's disease. In plasma, higher levels of oleic acid were associated with a reduced risk of MCI-to-AD progression. Higher levels of docosahexaenoic acid in CSF were associated with a lower risk of MCI-to-AD progression. Higher levels of docosahexaenoic acid in plasma were associated with a greater rate of MCI-to-AD progression.

Physicochemical Characterization of ‘Moroccan Picholine’ Olive (Olea europaea L.) Oil Produced in Southern Morocco Using Multivariate Statistical Analysis

This study focuses on evaluating the characteristics of olive oil produced in the Taroudant province (southern Morocco), making this the first comprehensive study focusing on olive oil from the ‘Moroccan Picholine’ cultivar. Our objective was to elucidate the distinctive qualities of olive oil from this region, providing valuable insights into its potential contributions to the country’s olive oil sector. For this purpose, several quality criteria (free fatty acids, moisture content, saponification value, and iodine value), oxidation indices (peroxide value, specific UV extinction coefficients, and oxidizability value), and purity indices (fatty acids and sterol composition) were evaluated. Our results reveal minor-to-significant variations (p &lt; 0.05) in the quality and oxidation parameters. Specifically, our results indicate diverse ranges for free fatty acids (0.33–3.62 g/100 g), peroxide values (0.85–4.01 mEq O2/kg oil), K232 (1.68 to 2.73), and K270 (0.09–0.34). Furthermore, consistently high levels of oleic acid (55.8 to 73.1%) and β-sitosterol (94.2 to 97%) were observed in the studied samples. These outcomes were confirmed through the use principal component analysis and hierarchical cluster analysis. Likewise, important correlations were outlined among the studied parameters. Multidimensional analyses not only highlight inherent variations, but also facilitate the classification of the analyzed olive oils into distinct categories. The results suggest that the Taroudant province exhibits favorable conditions for producing high-quality olive oil.

Assessment of Full-Fat Tenebrio molitor as Feed Ingredient for Solea senegalensis: Effects on Growth Performance and Lipid Profile.

Tenebrio molitor (TM) is considered as one of the most promising protein sources for replacing fish meal in aquafeeds, among other things because it is rich in protein, a good source of micronutrients and has a low carbon footprint and land use. However, the main drawback of TM is its fatty acid profile, in particular its low content of n-3 PUFA. This study evaluates the effects of partially replacing plant or marine-derived with full-fat TM meal at two different levels on growth performance and lipid profiles of Senegalese sole (Solea senegalensis). For this purpose, a control diet (CTRL) and four experimental isoproteic (53%) and isolipidic (16%) diets were formulated containing 5 and 10% TM meal replacing mostly fish meal (FM5 and FM10), or 10 and 15% TM meal replacing mostly plant meal (PP10 and PP15). Fish (215 g) were fed at 1% of their body weight for 98 days. The final body weight of fish fed the experimental diets containing TM meal was not different from that of fish fed the CTRL diet (289 g). However, the inclusion of TM meal resulted in a gradual improvement in growth rate and feed efficiency in both cases (replacement of fish or plant meals), and significant differences in specific growth rate (SGR) were observed between fish fed the CTRL diet (SGR = 0.30% day-1) and those fed diets with the highest TM meal content (PP15; SGR = 0.35% day-1). The experimental groups did not show any differences in the protein content of the muscle (19.6% w/w). However, significant differences were observed in the total lipid content of the muscle, with the FM10, PP10, and PP15 groups having the lowest muscle lipid contents (2.2% ww). These fish also showed the lowest neutral lipid content in muscle (6.6% dw), but no differences were observed in the total phospholipid content (2.6% dw). Regarding the fatty acid profile, fish fed FM10, PP10 and PP15 had lower levels of linoleic acid (18:2n-6) and higher levels of oleic acid (18:1n-9) in liver and muscle compared to fish fed CTRL. However, no differences were found between fish fed CTRL and TM-based diets for docosahexaenoic acid (22:6n-3) and total n-3 PUFA in liver and muscle. In conclusion, our study demonstrated that full-fat TM inclusion up to 15% in S. senegalensis diets had no negative effects or even some positive effects on fish survival, growth performance, nutrient utilization and flesh quality.

Effect of Growing Regions on Discrimination of Turkish-Style Black Table Olives from Gemlik Cultivar.

Gemlik is a cultivar that grows in a distinct region of Turkiye and is ideal for brine fermentation of brine black table olives. Bursa Protected Designated Origin (PDO) and Izmir non-PDO Gemlik table olives have high levels of oleic acid (74%), total phenol (190 mg/kg), and dry matter (57%), while being low in linoleic acid (8%). The pH values and salt contents were observed to be in the range of 4.1 to 4.3 and 3.9% to 4.8%, respectively. During the fermentation of Gemlik table olives, a mass transfer occurred, resulting in a reduction in reducing sugar and total sugar contents as well as an increase in the salt content of the olives. Despite the reduction of phenolic content in both Gemlik PDO and non-PDO table olives, their antioxidant capacity remains high after fermentation. The oil content, antioxidant activity, phenolic contents, palmitic, palmitoleic, oleic, and linoleic acids were all found to be significant variables in distinguishing between Gemlik PDO and non-PDO table olives using PLS-DA analysis. There is a statistically significant correlation between the phenolic content and oleic (0.588) and linoleic (-0.659) acids (p < 0.05). Bursa PDO and Izmir non-PDO exhibit enhanced nutritional quality and antioxidant activity, unequivocally differentiating them from Hatay and Mersin non-PDO Gemlik table olives with 98% accuracy through discriminant analysis (p < 0.05). PLS-DA and DA can effectively identify variations in the quality of Turkish-style black table olives preserved in brine, originating from PDO and non-PDO growing areas.

Extraction methods and geographical variability influence on phenolic content, fatty acid composition and physicochemical quality, of Moroccan Picholine olive oils in the Fes-Meknes region

The study aims was to analyze the influence of extraction method and geographical origin on the quality of olive oils. Thirty samples of the "Moroccan Picholine" variety were collected from three types of crushers (hydraulic press, two-phase and three-phase) in four cities of the main Moroccan olive-growing region, Fes-Meknes, during the 2021 and 2022 harvest seasons. Oils from this region are generally of virgin and extra virgin olive oils quality, according to the International Olive Council, with high levels of oleic acid reaching up to 80.12 ± 1.94% for oils produced in Meknes, two-phase triturations. Two-phase extracted oils from Fez and Meknes show the highest MUFA/PUFA (monounsaturated fatty acids/ polyunsaturated fatty acids) ratios and lower quality parameters, indicating richness in monounsaturated fatty acids and improved oxidative stability. Polyphenol levels varied significantly between the cities of Fez, Meknes, Taounate and Taza, reaching 307.31 ± 17.44 mg/kg GAE for Fez, with notable differences (p &lt; 0.05). In addition, chlorophyll and carotenoid levels differed significantly according to the type of extraction and the city of origin (p = 0.004 and p = 0.042, respectively). Principal component analysis identified three distinct groups based on extraction method and geographical location, highlighting the importance of these factors in obtaining olive oils with the specific characteristics sought.

Improving Shelf Life and Content of Unsaturated Fatty Acids in Meat of Lambs Fed a Diet Supplemented with Grape Dregs.

This study was conducted to evaluate the potential effects of dietary grape residue levels on the slaughter indicators, meat quality, meat shelf-life, unsaturated fatty acid content, and expression of fatty acid deposition genes in the muscle of lambs. Sixty 30-month-old male Dorper and Small-Tailed Han F1 hybrid lambs were assigned to a single factor complete randomized trial design and fed with four different diets including 0%, 8%, 16%, and 24% grape dregs, respectively. The findings regarding meat production efficacy in the lambs revealed substantial differences. The control group showed notably lower dressing percentage, carcass weight, net meat weight, meat percentage concerning carcass, meat-to-bone ratio, relative visceral and kidney fat mass, and rib eye area compared to the other groups (p < 0.05). Additionally, the meat shearing force of lambs fed a diet with 16% grape pomace (GP) was significantly higher than that of the 24% GP group (p < 0.05), while the 24 h meat color parameter a* value of the control group was notably higher than that of the 8% GP group (p < 0.05). In addition, compared to the control group, lambs fed with a diet containing 16% GP had higher levels of oleic acid (C18:1n-9c), linoleic acid (C18:2n-6c), behenic acid (C22:0), tricosanoic acid (C23:0), lignoceric acid (C24:0), and conjugated linoleic acid (CLA), at a ratio of ∑CLA/TFA, ∑n-6, ∑MUFA, and ∑PUFA in the longissimus dorsi muscle (p < 0.05), but the reverse case was applicable for Total Volatile Basic Nitrogen (TVB-N) content (p < 0.05). GP supplementation did not substantially affect the expression of stearoyl-CoA desaturase (SCD), peroxisome proliferator activated receptor alpha (PPARα), and peroxisome proliferator-activated receptor gamma (PPARγ) genes (p > 0.05). The findings indicated that incorporating grape dregs in the diets of fattening lambs leads to notable enhancements in meat production and the antioxidant capacity of lamb meat, and effectively extends the shelf life of the meat.

Breeding of high seed oil oleate levels into Upland cotton from wild Gossypium barbadense L. germplasm

AbstractA high seed oil oleate trait identified in wild Gossypium barbadense L. cotton accession GB713 (PI 608139) was bred into Upland cotton (Gossypium hirsutum L.) cultivar Sure‐Grow 747 (SG747, PVP 9800118). Starting from the F2 generation of an initial cross between GB713 and SG747, plants were selected by measuring the seed oil fatty acid distributions by gas chromatography. Selected F3 plants were crossed to M Rk‐Rn 6, a line previously created from GB713 and SG747 for improved nematode resistance. High‐oleate plants were selected in the F2 population. Selected plants were then crossed to HOa1 (a ∼35% oleate line derived from M Rk‐Rn 1, a sibling line to M Rk‐Rn 6), and high‐oleate plants were again selected in the F2 population. F3 plants were re‐selected for the high‐oleate trait, and four selected F4 lines were evaluated in 2022 field plots. The four lines had oleate levels between 52% and 57%, approximately triple that found in normal cottonseed oil (15%–19%) and higher than in either the high‐oleate wild GB713 parent (∼40%) or in the previously released HOa cotton plant lines (33%–35%). The increased oleate levels occurred with an approximately equal reduction in linoleic acid. Other seed and fiber properties were largely like those of SG747, although some residual GB713 character existed within the lines. The resulting oil composition should be ideal for use in deep fat frying, with the high level of oleic acid improving the oil's oxidative stability but with sufficient linoleic acid to maintain frying flavor.

FATTY ACID COMPOSITION OF NIGELLA SATIVA L. SEED OIL INTRODUCED IN DAGESTAN

Nigella sativa L. is an annual herbaceous plant grown not only as an ornamental plant, but also has been used in folk medicine. N. sativa seeds are a source of biologically active substances. This paper provides an analysis of the quantitative content of fatty acids in samples of various ecological and geographical origin. It is shown that seeds grown in Dagestan are characterized by a relatively high content of fatty oil. The characteristics of the qualitative and quantitative composition of fatty acids, depending on their origin, are given. The highest content of linoleic and stearic fatty acids was found in the sample from Egypt. Palmitic acid was the highest in the sample from Saudi Arabia, and oleic acid was the highest in the sample from Azerbaijan. The revealed differences in the fat content are of great importance, since samples for breeding purposes can be selected on their basis. In particular, the sample from Azerbaijan showed relatively high levels of oleic acid, which generally contributes to the increase in the antioxidant activity of vegetable oil and lengthens of the keeping period.

Molecular breeding to develop advanced lines with high oleic acid and pod yield in peanut

Peanut (Arachis hypogaea L.) is an important crop, and its seeds contain high amounts of protein and oil. The high oleic acid content of peanut oil provides prolonged shelf life and health benefits. Delta-12-desaturase (oleoyl-PC desaturase) is a key enzyme that catalyzes the conversion of oleic acid into linoleic acid and is coded by two homologs genes: ahFAD2A and ahFAD2B. It is known that plants with both point mutations (aabb) in these genes fail to produce Δ-12-desaturase enzyme cause to higher oleic acid. In this study, we employed marker-assisted breeding to develop high-oleic and high-pod-yield lines using a population sourced from crosses between a high-yield cultivar, NC-7 and an advanced high-oleic line, HOG. The oleic acid character of the parents was initially confirmed using molecular markers and fatty acid composition analyses. After crossing, two “true hybrids” were selected from the F1 generation with the use of an allele-specific marker. True hybrids were selfed and the fatty acids profiles of selected F2 plants with higher pod yield and seed length were determined. These two F2 populations (NH-2 and NH-3) having high levels of oleic acid were advanced to further generations. Single plants in F3 to F6 were selected and selfed based on seed yield and pod features. Finally, a total of 21 advanced inbred lines with high pod yields and oleic acid content of up to 83.3% were obtained. The ahFAD2B gene of selected high oleic lines were also sequenced and homozygous insertion was confirmed based on the reference genome. The oleic/linoleic ratio was increased to 39.0 with regard to cultivar NC-7, which was only 2.9. The advanced lines reported here showed combine high oleic content with desired agronomic traits and that this can meet the market demand for high-quality vegetable oil.

Oleanolic Acid Inhibits SCD1 Gene Expression to Ameliorate Fructose-Induced Hepatosteatosis through SREBP1c-Dependent and -Independent Mechanisms.

The mechanisms of oleanolic acid (OA) regulating hepatic sterol regulatory element-binding protein (SREBP) 1c/stearoyl-CoA desaturase (SCD) 1 pathway to ameliorate fructose-induced hepatosteatosis are investigated. Rats treated with 10% w/v fructose solution are co-administered by OA for 5 weeks, and then sacrifice after fasting for 14 h. OA reverses the fructose-induced increase in hepatic triglyceride (TG) content and downregulates Scd1 mRNA expression. However, two upstream transcription factors, ChREBP and SREBP1c, remain at normal levels with or without fructose and/or OA. In vivo and in vitro studies using SREBP1c-/- mice and HepG2 cell models show that OA also inhibits SCD1 gene overexpression and high hepatic TG levels induced by fructose. On the other hand, in SCD1-/- mice, when the fructose diet is supplemented with high levels of oleic acid (OLA) to compensate for the deficiency of SCD1, OA inhibits hepatic SREBP1c and lipogenic gene expression and reduces hepatic OLA (C18:1) production to improve fructose and/or OLA induced liver lipid deposition. Furthermore, OA promotes PPARα and AMPK to enhance fatty acid oxidation in fructose + OLA-fed SCD1-/- mice. OA may inhibit SCD1 gene expression to ameliorate fructose-induced hepatosteatosis through SREBP1c-dependent and -independent mechanisms.

Generation of Sesame Mutant Population by Mutagenesis and Identification of High Oleate Mutants by GC Analysis.

Sesame is one of the important oilseed crops in the world. Natural genetic variation exists in the sesame germplasm collection. Mining and utilizing the genetic allele variation from the germplasm collection is an important approach for seed quality improvement. The sesame germplasm accession, PI 263470, which has a significantly higher level of oleic acid (54.0%) than the average (39.5%), was identified by screening the entire USDA germplasm collection. The seeds from this accession were planted in a greenhouse. Leaf tissues and seeds were harvested from individual plants. DNA sequencing of the coding region of the fatty acid desaturase gene (FAD2) confirmed that this accession contained a natural mutation of G425A which may correspond to the deduced amino acid substitution of R142H leading to the high level of oleic acid, but it was a mixed accession with three genotypes (G/G, G/A, and A/A at the position). The genotype with A/A was selected and self-crossed for three generations. The purified seeds were used for EMS-induced mutagenesis to further enhance the level of oleic acid. A total of 635 M2 plants were generated from mutagenesis. Some mutant plants had significant morphological changes including leafy flat stems and others. M3 seeds were used for fatty acid composition analysis by gas chromatography (GC). Several mutant lines were identified with high oleic acid (70%). Six M3 mutant lines plus one control line were advanced to M7 or M8 generations. Their high oleate traits from M7 or M8 seeds harvested from M6 or M7 plants were further confirmed. The level of oleic acid from one mutant line (M7 915-2) was over 75%. The coding region of FAD2 was sequenced from these six mutants, but no mutation was identified. Additional loci may contribute to the high level of oleic acid. The mutants identified in this study can be used as breeding materials for sesame improvement and as genetic materials for forward genetic studies.

Abstract PR017: Differences in melanoma lipid metabolism among distinct metastatic sites

Abstract Metastasis is a very inefficient process in which few disseminated cancer cells survive. We developed a patient-derived xenograft assay in which melanomas engraft and spontaneously metastasize (Quintana Nature 456:593-598). Using this assay, we discovered that melanoma cells experience a spike in reactive oxygen species during metastasis and that distant metastasis is limited by oxidative stress (Piskounova Nature 527:186-191). Successfully metastasizing cells undergo reversible metabolic changes during metastasis that increase oxidative stress resistance. For example, melanoma cells in lymph experience less oxidative stress and form more metastases than melanoma cells in the blood (Ubellacker Nature 585:113-120). This is true in both patient-derived melanomas growing in immunocompromised mice and mouse melanomas growing in syngeneic immunocompetent mice. Cells metastasizing through blood, but not lymph, appear to undergo ferroptosis, a form of cell death marked by lipid oxidation. Multiple differences between lymph fluid and blood plasma may contribute to this difference in oxidative stress, including higher levels of oleic acid in lymph. Oleic acid is a monounsaturated fatty acid that protects melanoma cells from ferroptosis, apparently by reducing the abundance of oxidizable polyunsaturated fatty acids (PUFAs) in phospholipids. In the current study we found that some PUFAs sensitize melanoma cells to lipid peroxidation and cell death more than others. Moreover, we analyzed the lipid profiles of common sites of distant metastasis – liver, brain, kidney, pancreas, bone marrow, lung, and liver – and found that the PUFA compositions of these tissues varies significantly. This raises the possibility that metastasizing melanoma cells may incorporate more PUFAs into their phospholipid membranes and exhibit increased ferroptosis sensitivity in some metastatic sites as compared to others. We found that melanoma cells isolated from metastatic sites by flow cytometry had higher levels of PUFAs in their phospholipid membranes compared to those isolated from subcutaneous tissues. These cells isolated from distal sites also exhibited higher levels of lipid ROS and contained higher levels of oxidized lipid species compared to subcutaneous tumors. These data suggest that the lipid metabolism of metastatic tumors reflects both lipid availability in the metastatic site as well as cell-intrinsic differences in lipid metabolism, both of which contribute to lipid ROS. Both cell-intrinsic and cell-extrinsic lipid metabolism likely influence the ability of melanoma cells to survive in distinct metastatic sites during metastasis. Citation Format: Thomas P. Mathews, Sarah Muh, Arin B. Aurora, Sean J. Morrison. Differences in melanoma lipid metabolism among distinct metastatic sites [abstract]. In: Proceedings of the AACR Special Conference: Cancer Metastasis; 2022 Nov 14-17; Portland, OR. Philadelphia (PA): AACR; Cancer Res 2022;83(2 Suppl_2):Abstract nr PR017.

Processing and Utilization of Natural Phospholipids from Fish Belly Fat Waste of Jambal Siam Fish (Pangasius Hypophthalamus)

Phospholipids are used for food products, drug formulas, stabilizers, lubricants, cosmetics, pharmaceuticals, and emulsifiers. Synthetic phospholipids tend to be utilized for food and medicinal purposes, but nowadays consumer concern has shifted to phospholipids from natural ingredients. Phospholipids have functional properties towards health, consumer often requires phospholipids containing certain fatty acids such as omega 3. The purpose of this research is to process the belly fat waste of jambal siam fish into phospholipids and purify the phospholipids that are already obtained. The results showed that the highest saturated fatty acid content in jambal siam fish belly oil was palmitic acid which was expected to dominate the structure of phosphatidylglycerol, phosphatidic acid, and cardiolipin. The highest content of unsaturated fatty acids is oleic acid which was expected to dominate phosphatidylinositol, phosphatidyl ethanolamine, and phosphatidylcholine structure. The highest phospholipid content was phosphotidylcholine, followed by phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylinositol. The hexane solvent produced a higher soluble hexane unsaturated fatty acid fraction than the insoluble hexane fraction, especially omega 6 and omega 9. While acetone produced higher levels of oleic acid and omega 3 soluble acetone, which was higher than the insoluble acetone. The higher the level of purity of phospholipids, the higher the content of oleic acid and omega 3.

Evaluation of variability in seed coat color, weight, oil content, and fatty acid composition within the entire USDA‐cultivated peanut germplasm collection

AbstractNatural genetic diversity in plant germplasm collections provides the genetic variability for crop improvement. To explore this natural genetic variability, over 8,800 accessions within the USDA cultivated peanut (Arachis hypogaea L.) germplasm collection were evaluated for several seed quality traits including seed coat color, 100‐seed weight, oil content, and fatty acid composition. After removing contaminated and/or mixed accessions, the remaining 8,466 accessions were used for further data analysis. The mean of 100‐seed weight was 49.44 g with a wide range of 21.84 to 148.36 g (6.8‐fold difference). Six accessions (PI 414995, PI 648234, PI 318740, PI 336978, PI 320059, and PI 578229) with 100‐seed weight &gt;110 g were identified. The mean of oil content was 48.94% with a range of 36.51 to 59.45% (1.6‐fold difference). Seven accessions (PI 471949, PI 472001, PI 497369, PI 442739, PI 343423, PI 161298, and PI 668582) with oil content &gt;58% were identified. The mean of oleic acid was 45.2% with a wide range of 39.99 to 82.08% (2.1‐fold difference). The mean of linoleic acid was 32.56% with a wide range of 2.0 to 46.68% (23.3‐fold difference). Thirty‐seven accessions were identified with &gt;65% oleic acid. Among them, PI 636472 and PI 596800 had the highest level of oleic acid (82.1 and 81.8%, respectively). Significant variation in these traits was also identified between subspecies and among botanical varieties. Some significantly positive and negative correlations among these traits were also determined. The results presented here will be useful for peanut breeders, geneticists, and other users.

Physicochemical Study, Fatty Acid Profile and Antioxidant Potential of the Seed Oil of Polyalthia longifolia angustifolia

In the face of worsening malnutrition or undernutrition in poor countries, diversification of food resources remains a high priority for the World Health Organization (WHO). Thus, the present study aims to determine the phytochemical composition and antioxidant activity of the fat extracted from the seeds of Polyalthia longifolia angustifolia.&#x0D; The fatty acid profile of the oil, extracted from the seeds with hexane by the Soxhlet method, was determined by gas chromatography-mass spectrometry (GC/MS) analysis. The physicochemical parameters of the oil were measured according to AFNOR standards. The total phenol and flavonoid contents of the seeds were evaluated by UV-visible spectrophotometric measurement. Antioxidant activity of the oil was determined by the reduction test of ferric ions (Fe3+) of the TPTZ complex to ferrous ion (Fe2+). The results revealed that the oil contains a higher level of oleic acid (63.38%), followed by: palmitic acid (13.80%); linoleic acid (11.74%) and stearic acid (6.24%). The physicochemical parameters of the oil were: refractive index (1.499); acid value (87.69 mg KOH/g of oil); acidity (23.659 g oleic acid/100 g of oil); iodine value (78.26 g I2/100 g of oil); saponification value (179.741 mg KOH/g of oil) and peroxide number (10.85 meq O2 /kg of oil).&#x0D; In short, the seeds of P. l. angustifolia contain fat and phytochemicals with appreciable antioxidant activity. However, further studies are needed to evaluate the toxicity, nutritional and therapeutic properties of the oil before considering the different types of applications that can be made with this oil.

Proximate Composition, Physicochemical, and Lipids Profiling and Elemental Profiling of Rapeseed (Brassica napus L.) and Sunflower (Helianthus annuus L.) Grown in Morocco.

We investigate and compare the nutritional and physicochemical properties of rapeseed and sunflower grown in Morocco. In order to examine a complete physicochemical characterization, various parameters such as mineral profile, fatty acid composition, sterols contents, total flavonoids content (TFC), total polyphenols content (TPC), and quality oil parameters were evaluated. The results showed a relatively small difference in the physicochemical composition of the seeds, as sunflower seeds recorded higher amounts of protein and oil content (22.98 ± 0.01 g/100 g and 41.30 ± 0.50 g/100 g) than rapeseed (22.98 ± 0.01 and 38.80 ± 0.50), while mineral elements profile was observed to be statistically different. Nevertheless, both seeds were rich in K, Ca, P, Mg, and Na and they were relatively poor in Na, Fe, Mn, Cu, and Zn. The most represented macroelement was K with the amount of 7936.53 ± 63.87 mg/Kg in rapeseed and 7739.22 ± 59.50 mg/Kg in sunflower. On the other hand, Cu was present in the analyzed samples the least, mostly below 20 mg/kg. For TPC and TFC, the sunflower recorded higher values (49.73 ± 0.50 and 25.37 ± 0.39 mg GAE/g) than rapeseed (38.49 ± 0.24 and 22.55 ± 1.76 mg QE/g). The fatty acid composition showed that both extracted oils have beneficial proprieties, as they are rich in unsaturated fatty acids; namely, rapeseed oil contains a high level of oleic acid (C18 : 1) (62.19%), while sunflower oil was richer in linoleic acid (C18 : 2) (55.7%). As a result, we conclude that the studied varieties have major importance in terms of both nutritional and seed improvement potentials.