This study aimed to investigate the relationship between various job stressors and health-related quality of life among female nursing assistants working in long-term care facilities. A cross-sectional study was conducted in Taiwan. Data were collected using a structured, well-designed, pre-tested questionnaire with background questions and questions about job stressors and health-related quality of life as measured by SF-12. Our empirical results show that nursing assistants with higher scores for job control and work-related social support tend to enjoy better mental health, as indicated by higher mental component summary scores. Additionally, nursing assistants with higher psychological demand scores tend to have worse overall health, as indicated by lower physical component summary and mental component summary scores. We suggest reducing selected job stressors and enhancing job control to improve nursing assistants' health-related quality of life.