High Mass Research Articles

Subclinical hypothyroidism and metabolic syndrome in psychiatric patients: A systematic literature review and meta-analysis.

The systematic review evaluated the association of subclinical hypothyroidism (SCH) with metabolic syndrome (MetS) and specific MetS components in people with major psychiatric disorders. A systematic review and meta-analysis was conducted to evaluate the association of SCH with MetS and its components in people with major psychiatric conditions. Five studies incorporating 24,158 participants met the inclusion criteria. All five studies comprised patients with depression and/or anxiety. Three studies incorporating 3365 participants were suitable for the meta-analysis. The pooled Odds Ratio (OR) of MetS was 3.46 (95% Confidence Interval/CI = 1.39-8.62) in major depressive disorder (MDD) and anxiety disorders patients with concurrent SCH compared to those without SCH. Meta-analysis showed a significant positive association between SCH and high body mass index (OR = 2.58, 95%CI = 1.33-5.01), high fasting plasma glucose (OR = 3.05, 95%CI = 1.79-5.18) and low high-density lipoprotein cholesterol (OR = 2.30, 95%CI = 1.82-2.92). These findings suggest a significant positive association between MetS and SCH in people with MDD and anxiety disorders. This review informed the clinical implications of MetS in MDD with comorbid SCH and the importance of early diagnosis and treatment for SCH and MetS in psychiatric patients.

Common origin for black holes in both high mass X-ray binaries and gravitational-wave sources

Common origin for black holes in both high mass X-ray binaries and gravitational-wave sources

Insight into the mechanism of adsorption and release of 11-nor-9-carboxy-Δ9-tetrahydrocannabinol in sewage by modeling regional suspended particles for evaluation of the influence on monitoring of cannabis illicit abuse

The adsorption of 11-nor-9-carboxy-∆9-tetrahydrocannabinol (THC-COOH) by the suspended particles in sewage makes it fail to accurately monitor cannabis abuse. In this work, the model sewage sample was prepared through equivalent mixing the sewage from 10 different sewage treatment plants in Guangdong province of China and used as a comprehensive representative for investigating the adsorption and release behavior of THC-COOH on the suspended particles under different temperature and pH. The solid-liquid distribution of THC-COOH in sewage depended strongly on the adsorption and release properties which were susceptible to the temperature and pH, specially adjusting pH to 11.0 could release more than 90% of THC-COOH from the suspended particles. By means of the kinetics models, pseudo-second-order kinetic and Weber-Morris models revealed the mechanism of adsorption and release of THC-COOH in sewage that was a relatively reversible and controllable process with multiple interactions, and then it was further confirmed by the validation experiment in a variety of actual sewage samples. According to the suggested pH, the modification of the existing detection protocol prior to high performance liquid chromatography-tandem triple quadrupole mass spectrometry (HPLC-TQ-MS/MS), was successfully applied to determination of THC-COOH in the stimulated positive samples, and the recoveries and RSDs were respectively 95.48-99.79% and 4.0-5.6%. The finding could greatly help improving the accuracy of not only the detection of THC-COOH in sewage but also the estimation data of the consumption level of cannabis in the related regions.

Global Years Lived with Disability for Musculoskeletal Disorders in Adults 70 Years and Older from 1990 to 2019, and Projections to 2040

In this article, we provided a comprehensive overview and in-depth analysis of global patterns and temporal trends in years lived with disability (YLDs) for musculoskeletal (MSK) disorders in individuals aged ≥70. Data on YLDs for MSK disorders in individuals aged ≥70 were obtained from the Global Burden of Disease 2019. The average annual percentage change (AAPC) was calculated to assess the temporal trends in the YLDs rate of MSK disorders. A Bayesian Age-Period-Cohort model was used to predict the YLDs rate up to the year 2040. In 2019, the global rate of YLDs for MSK disorders in individuals aged ≥70 were 4,819.81 (95% UI: 3,402.91 - 6,550.77) per 100,000 persons. The YLDs rate of MSK disorders in female was 1.36 times higher than that in male, and was highest in high SDI regions. From 1990 to 2019, the global YLDs rate showed a slightly downward trend (AAPC=-0.04%, 95% CI: -0.06% to -0.03%), while it significantly increased in high, low-middle, low SDI regions. Tobacco and high body mass index were the primary risk factors worldwide, while in low SDI regions, occupational risks emerged as the predominant factors. Up to 2040, the global YLDs rate of MSK disorders are expected to increase by 1.78%, with 36.39%, 20.66%, 18.96% and 5.32% growth in other MSK disorders, rheumatoid arthritis, neck pain and osteoarthritis. MSK disorders are a significant and continuously growing public health concern among older adults. Tailored interventions should be developed for older adults, taking into account the variations across distributions, trends, and risk factors in terms of sex and SDI levels.

Understanding proteome quantification in an interactive learning module on Google Cloud Platform.

This manuscript describes the development of a resource module that is part of a learning platform named 'NIGMS Sandbox for Cloud-based Learning' https://github.com/NIGMS/NIGMS-Sandbox. The overall genesis of the Sandbox is described in the editorial NIGMS Sandbox at the beginning of this Supplement. This module delivers learning materials on protein quantification in an interactive format that uses appropriate cloud resources for data access and analyses. Quantitative proteomics is a rapidly growing discipline due to the cutting-edge technologies of high resolution mass spectrometry. There are many data types to consider for proteome quantification including data dependent acquisition, data independent acquisition, multiplexing with Tandem Mass Tag reporter ions, spectral counts, and more. As part of the NIH NIGMS Sandbox effort, we developed a learning module to introduce students to mass spectrometry terminology, normalization methods, statistical designs, and basics of R programming. By utilizing the Google Cloud environment, the learning module is easily accessible without the need for complex installation procedures. The proteome quantification module demonstrates the analysis using a provided TMT10plex data set using MS3 reporter ion intensity quantitative values in a Jupyter notebook with an R kernel. The learning module begins with the raw intensities, performs normalization, and differential abundance analysis using limma models, and is designed for researchers with a basic understanding of mass spectrometry and R programming language. Learners walk away with a better understanding of how to navigate Google Cloud Platform for proteomic research, and with the basics of mass spectrometry data analysis at the command line. This manuscript describes the development of a resource module that is part of a learning platform named ``NIGMS Sandbox for Cloud-based Learning'' https://github.com/NIGMS/NIGMS-Sandbox. The overall genesis of the Sandbox is described in the editorial NIGMS Sandbox [1] at the beginning of this Supplement. This module delivers learning materials on the analysis of bulk and single-cell ATAC-seq data in an interactive format that uses appropriate cloud resources for data access and analyses.

Temperature and Pressure Prediction of Offshore High CO2-Content Associated Gas Lift and Analysis of Influencing Factors

With the continuous injection of CO2 into the reservoir, a portion of the sequestered CO2 is co-produced with crude oil, resulting in a significant concentration of CO2 in the associated gas. Utilizing high CO2-content associated gas for offshore gas lift operations improves the reutilization of such gas and reduces costs related to sourcing and transporting it. To ensure smooth activation of the gas lift valve, conducting a thorough study on temperature and pressure within the wellbore during the gas lift process is essential. This study developed a dynamic model for calculating the transient temperature and pressure within offshore gas lift wellbores and fitted equations to calculate the physical properties of high CO2-content associated gas for specific compositions. This refinement minimized computational discrepancies arising from fluctuations in physical property parameters. When applying this model to oilfield scenarios, the maximum error observed was 3.83%, which aligned well with the accuracy standards required for oilfield computations. By integrating oilfield production data, this research explored the influence of injection parameters on temperature and pressure dynamics within the gas lift wellbore. The results revealed that variations in injection temperature and pressure had minimal impact on the temperature at the gas lift valve. Yet, they induced significant changes in the pressure at that point. An increase in CO2 concentration within the gas source composition was associated with considerable shifts in temperature and pressure gradients throughout the wellbore. At high mass flow rates, the temperature gradient of the wellbore was notably reduced, while the pressure gradient was significantly enhanced. These research findings provided a theoretical basis for optimizing gas lift operations using high CO2-content associated gas in offshore settings.

Hollow Fiber Contactors with Improved Hydrophobicity for Acid Gas Removal: Progress and Recent Advances

The gas–liquid membrane contactor technology, which integrates the absorption process with membranes, is a developing membrane technology that is especially pertinent to acid gas absorption. When it comes to removing acid gases from natural gas or after combustion, membrane technology has demonstrated potential as a substitute for conventional absorption columns. The membrane contactor offers exceptional operating flexibility and a high mass transfer area. In addition to summarizing the key elements of membrane materials, absorbents, and membrane contactor design, this paper presents the working principle and wetting mechanism of hollow membrane contactors and focuses the most recent advancements in membrane contactor research in gas separation from gas mixtures. The state-of-the-art overview of highly hydrophobic microporous membranes is presented after a discussion of the main challenges to the preparation of superhydrophobic membranes.

Vertical VOPO4·2H2O Nanosheets with Richly Exposed (200) Plane Enable Fast-Kinetics High-Mass-Loading Cathodes for Zn-Ion Batteries.

Extending the layer spacing of the (001) planes to regulate the mobility of Zn2+ is widely adopted to optimize the performance of VOPO4·2H2O cathode for zinc-ion batteries. However, the unique function originating from other planes is often neglected. Herein, an effective in situ conversion methodology is proposed for the synthesis of the (200) oriented growth of vertical VOPO4·2H2O nanosheets with oxygen vacancies (VOd-VOPO4). Theoretical simulation and ex situ characterizations collaboratively demonstrate that the richly exposed (200) plane with tetragonal channels can offer quick pathways for in-layer and cross-layer migration of Zn2+, exhibiting enhanced transfer kinetics with improved reversible capacity. Meanwhile, efficient electron migration in VOd-VOPO4 is guaranteed by the introduction of oxygen vacancies. Thus, the as-prepared VOd-VOPO4 harvests exceptional discharge capacity, impressive rate capability, and remarkable long-cycle stability at high mass loading. Notably, the VOd-VOPO4 electrode (15mg cm-2) provides a capacity of 213.5mAhg-1 with an ultrahigh areal capacity of 3.02 mAh cm-2 at 0.1 A g-1, showing great potential for applications. This study highlights the orientated growth strategy for facilitating ion storage and migration, offering novel perspectives on the development of high-performance electrodes and beyond.

10-year outcome after Roux-en-Y gastric bypass vs. duodenal switch with high body mass index: a randomized clinical study

10-year outcome after Roux-en-Y gastric bypass vs. duodenal switch with high body mass index: a randomized clinical study

The tubular baffled reactor and its potential for the biological methanation of carbon dioxide

The biological Power to Methane process (PtM) is gaining ground as an answer to the long-term renewable energy storage problem. Methane is an efficient hydrogen carrier, has an established worldwide transport infrastructure and can serve as a link between renewable power generation and a circular carbon economy. One of the defining factors regarding the scalability of the PtM process is the design of the reactor as it can determine the production rate/energy expenditure ratio. The tubular baffled reactor, a popular reactor design within the chemical industry has been assessed in the present study as a biomethanation reactor for the first time. The experiments were conducted with mixed cultures and the results point to high gas-liquid mass transfer capabilities as indicated by the methanation rates achieved (>90 % CH4 at 270 L/L/d mixed gas input rate). The gas/liquid flow ratio appears to have a stronger effect on methanation than the gas residence time. The working length of the reactor determines the pressure drop experienced by the culture, with higher pressure drops showing a negative correlation to methanogenesis.

Adopting adalimumab combined surgery in the management of moderate to severe hidradenitis suppurativa: Experience from a single medical center in southern Taiwan.

Hidradenitis suppurativa (HS) is a chronic inflammatory disorder primarily affecting the intertriginous and anogenital regions. Guidelines recommend various treatments for HS, including biologic agents like adalimumab for moderate to severe cases. Adalimumab is a type of human monoclonal IgG1 antibody designed to target tumor necrosis factor α. Recent studies have shown the effectiveness of adalimumab, either alone or combined with surgery, in managing HS. We retrospectively analyzed the medical chart of HS patients in a southern Taiwan medical center from 2019 to 2022 and investigated clinical features and treatment response. The institutional review board at Chang Gung Medical Foundation granted approval for the study. We primarily focused on moderate to severely affected patients. One hundred and two clinically diagnosed HS patients participated, with a male-to-female ratio of 2:1 and an average age of 31.8 at diagnosis. Among them, 41.2% were in Hurley stage III and 32.4% in stage II. Nineteen patients received excision with pre-surgical adalimumab; their average age at diagnosis was 31.1, with a gender ratio of 5.3:1. Surgery was most common on the buttocks (68%), axillae (21%), and groin (10%). Excision patients were primarily in advanced stages (Hurley III 94.7%, II 5.3%) with high body mass index. Adalimumab and surgery combined yielded a 68.4% improvement rate, while 15.8% remained stable and 15.8% did not respond as expected. In addition, smoking and obesity were prevalent among patients. Adalimumab showed promising results in moderate to severe HS, with significant improvement observed in our cases. The combination of adalimumab and surgery appeared effective in advanced HS patients with larger involved areas and more tunnels. No severe adverse events were reported. However, our study was limited by its retrospective nature and the lack of a control group. Despite these limitations, our study revealed the benefits of integrating adalimumab with suitable surgical procedures in managing patients experiencing moderate to severe HS in real-world scenarios.

Polyprenylated acylphloroglucinols from Hypericum sampsonii with cytotoxicity against pancreatic carcinomas

Chemical investigation on the 80% EtOH extract of the air dried aerial parts of Hypericum sampsonii resulted in the isolation of two new polycyclic polyprenylated derivatives, hypersampines A and B (1 and 2). The structures of the new compounds were elucidated by spectroscopic data (NMR, IR, and UV) and high resolution mass analysis. The two isolated polyprenylated acylphloroglucinols were tested in vitro for cytotoxic activities against 6 pancreatic cell lines. As a result, compounds 1 and 2 possessed modest cytotoxic activities against all the tested tumor cell lines with IC50 values less than 40 μM.

Wood derived carbon embedded with shell-core CoP@CoFe for efficient oxygen evolution

Development of hybrid materials with specific architecture, interface, and multicomponent interactions demonstrates effective paradigm for pursuing high-performance energy conversion materials. Herein, using renewable and inexpensive wood-derived carbon as host, the shell-core CoP@CoFe particles are embedded on walls of carbonized wood channels (termed as CoP@CoFe/CW) to fabricate composite catalyst for electrocatalysis oxygen evolution reaction (OER). With benefits of the well distribution of CoP@CoFe particles, high conductivity of CW with channels, and good integration between them, highly exposed catalysis surface as well as rapid charge/mass transfer are achieved. The interface effect of shell-core hybrid activates the intrinsic OER efficiency of CoP@CoFe. Thus, the designed CoP@CoFe/CW yields excellent OER performance with ultralow overpotential of 120 mV (10 mA cm−2). This strategy of catalyst design based on eco-friendliness and renewable CW host may offer promising reference to explore composite materials with high conductivity and fast mass transfer for water electrolysis and other energy storage/conversion systems.

A Triple Crosslinked Binder with Hierarchical Stress Dissipation and High Ionic Conductivity for Advanced Silicon Anodes in Lithium-ion Batteries.

Silicon (Si) is a promising anode material for high-energy-density lithium-ion batteries, but the significant volume change of Si particles during alloying/dealloying with lithium (Li) undermines the mechanical integrity of Si anode, causing electrode fracture, delamination and rapid capacity decay. Herein, a robust triple crosslinked network (TCN) binder with high ionic conductivity and hierarchical stress dissipation is reported for Si anodes, which is prepared by in situ chemical crosslinking polyacrylic acid (PAA) and melamine (MA). The triple interactions of hydrogen bonds, electrostatic interactions, and covalent amide bonds enhance the adhesion of binder to Si and synergistically promote stress dissipation within Si anodes, thus strengthening the dynamic structural stability of Si anodes during cycling. Moreover, the rapid coupling/decoupling of Li+ with the TCN binder enables an impressive Li+ transference number of 0.63 and high ionic conductivity of 1.2 × 10-4 Scm-1. Consequently, the Si-TCN anode delivers specific capacity of 2268 mAhg-1 with a high mass loading of 2mgcm-2, high-rate performance of 1673 mAhg-1 at 5 Ag-1, and stable cycling for 250 cycles at 1 Ag-1, thus showing great prospects for high-energy-density Si-based batteries.

Empowering the rapid authentication of the botanical origin of monofloral honey by coated blade spray mass spectrometry (CBS-MS)

In the quest for a rapid and cost-effective tool for determining the botanical origin of monofloral honey, the analytical capabilities of coated blade spray mass spectrometry (CBS-MS) were investigated. To this aim, the chemical profiles of 64 honey samples from seven different botanical origins (acacia, dandelion, chestnut, rhododendron, citrus, sunflower, and linden) were captured by the absorbent of the coated blades and then analyzed by mass spectrometry. An exploratory analysis was performed by principal component analysis (PCA) to generate a graphical representation of the CBS-MS data that allows the discovery of patterns, outliers, or relations between types of honey in an unsupervised manner. Additionally, the performances of four different classification algorithms (least absolute shrinkage and selection operator (LASSO), random forest (RF), and neural network (NNET) partial least squares discriminant analysis (PLS-DA)) were built up and compared. The performances of the four classifiers were verified by a 50 times-repeated, 5-fold-cross-validation and permutation test. Although all classifiers performed well, the RF showed significantly higher performances in cross-validation (with area under the curve (AUC) of 0.99, overall accuracy 0.94, Kappa 0.93, sensitivity 0.94, and specificity 0.99). Moreover, the permutation tests showed the models were not overfitted. Finally, to determine the molecular identities of the ions that most contribute to the classification, extracts from the same honey samples were prepared, analyzed by liquid chromatography coupled to high resolution tandem mass spectrometry (LC-HRMS/MS), and the most significant features were annotated. This proof-of-principle work warrants a future large-scale study to validate and challenge this CBS-MS-based method with a greater number of honeys from different years and geographical origins.

Ligand effect of PdRu on Pt-enriched surface for glucose complete electro-oxidation to carbon dioxide and abiotic direct glucose fuel cells.

The development of advanced electrocatalysts for the abiotic direct glucose fuel cells (ADGFCs) is critical in the implantable devices in living organisms. The ligand effect in the Pt shell-alloy core nanocatalysts is known to influence the electrocatalytic reaction in interfacial structure. Herein, we reported the synthesis of ternary Pt@PdRu nanoalloy aerogels with ligand effect of PdRu on Pt-enriched surface through electrochemical cycling. Pt@PdRu aerogels with optimized Pt surface electronic structure exhibited high mass activity and specific activity of Pt@PdRu about 450 mA·mgPt-1 and 1.09 mA·cm-2, which were 1.4 and 1.6 times than that of commercial Pt/C. Meanwhile, Pt@PdRu aerogels have higher electrochemical stability comparable to commercial Pt/C. In-situ FTIR spectra results proved that the glucose oxidation reaction on Pt@PdRu aerogels followed the CO-free direct pathway reaction mechanism and part of the products are CO2 by completed oxidation. Furthermore, the ADGFC with Pt@PdRu ultrathin anode catalyst layer showed a much higher power density of 6.2 mW·cm-2 than commercial Pt/C (3.8 mW·cm-2). To simulate the blood fuel cell, the Pt@PdRu integrated membrane electrode assembly was exposed to glucose solution and a steady-state open circuit of approximately 0.6 V was achieved by optimizing the glucose concentration in cell system.

A hot-Jupiter progenitor on a super-eccentric retrograde orbit.

Giant exoplanets orbiting close to their host stars are unlikely to have formed in their present configurations1. These 'hotJupiter' planets are instead thought to have migrated inward from beyond the ice line and several viable migration channels have been proposed, including eccentricity excitation through angular-momentum exchange with a third body followed by tidally driven orbital circularization2,3. The discovery of the extremely eccentric (e = 0.93) giant exoplanet HD 80606 b (ref. 4) provided observational evidence that hot Jupiters may have formed through this high-eccentricity tidal-migration pathway5. However, no similar hot-Jupiter progenitors have been found and simulations predict that one factor affecting the efficacy of this mechanism is exoplanet mass, as low-mass planets are more likely to be tidally disrupted during periastron passage6-8. Here we present spectroscopic and photometric observations of TIC 241249530 b, a high-mass, transiting warm Jupiter with an extreme orbital eccentricity of e = 0.94. The orbit of TIC 241249530 b is consistent with a history of eccentricity oscillations and a future tidal circularization trajectory. Our analysis of the mass and eccentricity distributions of the transiting-warm-Jupiter population further reveals a correlation between high mass and high eccentricity.

Non-monotonic relations of galaxy star formation, radius, and structure at fixed stellar mass

Abstract We investigate the relation between galaxy structure and star formation rate (SFR) in a sample of ∼2.9 × 104 central galaxies with z < 0.0674 and axial ratios b/a > 0.5. The star-forming main sequence (SFMS) shows a bend around the stellar mass of M* ≤ Mc = 2 × 1010M⊙. At M* ≤ Mc the SFMS follows a power-law $\text{SFR}\propto {}M_\ast ^{0.85}$, while at higher masses it flattens. Mc corresponds to a dark matter halo mass of Mvir ∼ 1011.8M⊙ where virial shocks occurs. Some galaxy structure (e.g., half-light radius, Re) exhibits a non-monotonic dependence across the SFMS at a fixed M*. We find $\text{SFR}\propto {R_e^{-0.28}}$ at fixed M*, consistent with the global Kennicutt-Schmidt (KS) law. This finding suggests that galaxy sizes contribute to the scatter of the SFMS. However, at M* > Mc the relationship between SFR and Re diminishes. Low-mass galaxies above the mean of the SFMS have smaller radii, exhibit compact and centrally concentrated profiles resembling green valley (GV) and quiescent galaxies at the same mass, and have higher $M_{\text{H}_2}/M_\rm{HI}$. Conversely, those below the SFMS exhibit larger radii, lower densities, have no GV or quiescent counterparts at their mass and have lower $M_{\rm{H}_2}/M_\rm{HI}$. The above data suggest two pathways for quenching low-mass galaxies, M* ≤ Mc: a fast one that changes the morphology on the SFMS and a slow one that does not. Above Mc, galaxies below the SFMS resemble GV and quiescent galaxies structurally, implying that they undergo a structural transformation already within the SFMS. For these massive galaxies, CG are strongly bimodal, with SFMS galaxies exhibiting negative color gradients, suggesting most star formation occurs in their outskirts, maintaining them within the SFMS.

Elucidating the antimicrobial and anticarcinogenic potential of methanolic and water extracts of edible Tragopogon coelesyriacus Boiss.

AbstractTragopogon coelesyriacus is a pharmacotherapeutic herbaceous plant belonging to the Asteraceae family and consumed as a vegetable. Here, the methanolic and water extracts of T. coelesyriacus were obtained from its aboveground parts (stem, leaves, and flowers), and the phytochemical potentials were investigated by LC‐HRMS (liquid chromatography–high resolution mass spectrometry) analysis for the first time. The antibacterial, antifungal, and anticarcinogenic activities of T. coelesyriacus extracts were investigated using experimental and in silico methods. T. coelesyriacus methanol extract revealed remarkable inhibitory activity against Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumonia (MICs = 0.83, 1.67, and 1.67 mg/mL, respectively) compared to Escherichia coli and Enterobacter aerogenes (MIC = 53.3 mg/mL). Inhibitory effects of T. coelesyriacus methanolic extracts were also observed in all Candida species tested, with the highest inhibition on Candida krusei (MIC = 0.83 mg/mL), whereas no inhibitory effect was identified from the water extract. Additionally, both T. coelesyriacus methanolic (IC50 = 86 μg/mL) and water (IC50 = 92 μg/mL) extracts revealed significant selective anticarcinogenic effects on AR42J pancreatic cancer cells. HeLa and MDA‐MB‐231 cells were, however, more resilient to methanol and water extract, respectively. In silico analyses further elucidated the noteworthy antibacterial potential of keracyanin chloride on S. aureus MurB enzyme and the remarkable inhibitory potential of naringin on FYN kinase specific for pancreatic cancer (AR42J) development. In conclusion, T. coelesyriacus phytochemicals with antibacterial, antifungal, and anticancer properties were revealed for the first time, and molecular docking studies on potential targets confirmed good agreement with experimental findings. Therefore, the current studies on T. coelesyriacus provide the basis for investigating new pharmaceutical potentials of other Tragopogon members.

The spatial distribution of components in Moringa oleifera (Lam) seed by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)

In this paper, 70 % methanol was used as solvent to extract the principal components from Moringa oleifera (Lam) seed with the ratio (w/v, 1:10) by ultrasonic-assisted extraction. The extract in Lam seed was detected by high resolution mass spectrometry and MS/MS spectra. The result suggests that there are three principal components including malic acid, d-trehalose and 4-(α-L-rhamnopyranosyloxy) benzyl glucosinolate in this extract. Based on this result, the spatial distribution of these components in Lam seed was visually obtained by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). There is much malic acid in the center of endosperm in Lam kernel and little in the shell of Lam seed. d-trehalose is mainly distributed in the seed coat of Lam seed, and almost no d-trehalose is distributed in the kernel of Lam seed. 4-(α-L-rhamnopyranosyloxy) benzyl glucosinolate is distributed in the seed coat and endosperm of Lam seed. The relative content of this compound in the seed coat of Lam seed is much higher than that in the endosperm. The result further suggests that the different component in Lam seed is distributed in different part. This study can provide some valuable reference for efficient extracting active components from Lam.