High Light Conditions Research Articles

The structure of the Physcomitrium patens photosystem I reveals a unique Lhca2 paralogue replacing Lhca4.

The moss Physcomitrium patens diverged from green algae shortly after the colonization of land by ancient plants. This colonization posed new environmental challenges, which drove evolutionary processes. The photosynthetic machinery of modern flowering plants is adapted to the high light conditions on land. Red-shifted Lhca4 antennae are present in the photosystem I light-harvesting complex of many green-lineage plants but absent in P. patens. The cryo-EM structure of the P. patens photosystem I light-harvesting complex I supercomplex (PSI-LHCI) at 2.8 Å reveals that Lhca4 is replaced by a unique Lhca2 paralogue in moss. This PSI-LHCI supercomplex also retains the PsaM subunit, present in Cyanobacteria and several algal species but lost in vascular plants, and the PsaO subunit responsible for binding light-harvesting complex II. The blue-shifted Lhca2 paralogue and chlorophyll b enrichment relative to flowering plants make the P. patens PSI-LHCI spectroscopically unique among other green-lineage supercomplexes. Overall, the structure represents an evolutionary intermediate PSI with the crescent-shaped LHCI common in vascular plants, and contains a unique Lhca2 paralogue that facilitates the moss's adaptation to low-light niches.

Characterization of photosystem II assembly complexes containing ONE-HELIX PROTEIN1 in Arabidopsis thaliana.

The assembly process of photosystem II (PSII) requires several auxiliary proteins to form assembly intermediates. In plants, early assembly intermediates comprise D1 and D2 subunits of PSII together with a few auxiliary proteins including at least ONE-HELIX PROTEIN1 (OHP1), OHP2, and HIGH-CHLOROPHYLL FLUORESCENCE 244 (HCF244) proteins. Herein, we report the basic characterization of the assembling intermediates, which we purified from Arabidopsis transgenic plants overexpressing a tagged OHP1 protein and named the OHP1 complexes. We analyzed two major forms of OHP1 complexes by mass spectrometry, which revealed that the complexes consist of OHP1, OHP2, and HCF244 in addition to the PSII subunits D1, D2, and cytochrome b559. Analysis of chlorophyll fluorescence showed that a major form of the complex binds chlorophyll a and carotenoids and performs quenching with a time constant of 420ps. To identify the localization of the auxiliary proteins, we solubilized thylakoid membranes using a digitonin derivative, glycodiosgenin, and separated them into three fractions by ultracentrifugation, and detected these proteins in the loose pellet containing the stroma lamellae and the grana margins together with two chlorophyll biosynthesis enzymes. The results indicated that chlorophyll biosynthesis and assembly may take place in the same compartments of thylakoid membranes. Inducible suppression of the OHP2 mRNA substantially decreased the OHP2 protein in mature Arabidopsis leaves without a significant reduction in the maximum quantum yield of PSII under low-light conditions, but it compromised the yields under high-light conditions. This implies that the auxiliary protein is required for acclimation to high-light conditions.

Small antisense RNA ThfR positively regulates Thf1 in Synechocystis sp. PCC 6803

Thylakoid formation1 (Thf1), encoded by sll1414 (thf1), is a multifunctional protein conserved in all photosynthetic organisms. thf1 expression is highly induced by high light in Synechocystis during photosynthesis-related stress. In this study, differential RNA sequencing analysis of the Synechocystis sp. PCC 6803 revealed a small antisense RNA (asRNA) gene located on the reverse-complementary strand of the thf1 gene. The full length of this asRNA (designated ThfR) was determined by 5′ and 3′ RACE analysis. The accumulation of thf1 mRNA was up-regulated synchronously with the ThfR level during survival after high-light stress or nitrogen starvation. Under nitrogen starvation or high-light stress, compared with the wild type, a ThfR overexpression mutant demonstrated relatively more Thf1 protein content, while a ThfR reduced-expression mutant accumulated less Thf1 protein. Furthermore, the overexpression of ThfR enhanced the electron transport rate and the proliferation of cyanobacteria under high-light stress. These results, which we confirmed further using an Escherichia coli sRNA expression platform, suggest that the thf1 gene is positively regulated by ThfR, possibly through protection of the RAUUW element at the RNase E cleavage site. This study represents the first report, to our knowledge, of a cis-transcript antisense RNA that targets thf1 in Synechocystis sp. PCC 6803 and provides evidence that ThfR regulates photosynthesis by positively modulating thf1 under high-light conditions.

Trait coordination at leaf level explains the resistance to excess light stress in shade-tolerant tropical tree species.

Plant functioning in response to the environment is an important issue for vegetation reassembly of degraded lands because of both low and excessive sunlight influence the performance of young plants. However, how shade-tolerant tree species deal with excess of light energy remains unclear due to the contrasting results among studies and the subjective classifications of species shade tolerance. From a quantitative classification of shade tolerance of 12 tropical tree species planted in the field under contrasting light conditions, we hypothesized that shade-tolerant species are capable of effective long-term acclimation to high-light conditions. Plant size and survival of species were measured at the beginning and 38months after planting. We also measured functional traits associated with carbon economy and non-photochemical energy dissipation. Under high-light condition, more light-demanding species showed higher growth rates associated with higher values of functional traits that improve the CO2 assimilation capacity. By contrast, more shade-tolerant species showed higher survival that was associated with higher values of qN, leaf K contents and leaf thickness. The concomitant increase in these traits, as well as their greater plasticity to light in WUEi, Fv/Fm and qN, confers on these species strong photoprotection to avoid high-light stress and to persist under open field conditions. The results challenge the common assumption that only fast-growing and light-demanding tree species are suitable for vegetation reassembly in full sunlight conditions and to improve the environmental conditions for other species.

Detecting mangrove photosynthesis with solar-induced chlorophyll fluorescence

ABSTRACT Accurately understanding the photosynthesis of mangroves is essential for inferring how climate change influences the terrestrial and marine carbon cycle. However, photosynthesis of mangroves remains poorly understood due to the difficulty of in situ flux data acquisition. Remote sensing of solar-induced chlorophyll fluorescence (SIF) provides a direct proxy for photosynthesis which would be an effective tool for detecting mangrove photosynthesis. We presented the first SIF observations from both ground and unmanned aerial vehicle (UAV) platforms in a mangrove forest and provided an exploratory attempt to downscale SIF from canopy level to leaf level to better characterize photosynthesis. We found that relationships between mangrove photosynthesis and SIF vary by light condition, which exhibited a negative correlation under low-light condition but positive correlation under high-light condition. Moreover, we found that inter-species differences affect the relationship between photosynthesis and SIF in mangroves. Relationship exhibited more clear-cut correlation by distinguishing mangrove species than considering them as a whole. Our results would help to better understand the photosynthesis mechanism of the mangrove forests and fills the gap of the relationship between SIF and photosynthesis in subtropical and tropical wetland ecosystems.

Autophagy Induced Accumulation of Lipids in pgrl1 and pgr5 of Chlamydomonas reinhardtii Under High Light.

Chlamydomonas (C.) reinhardtii is a potential microalga for lipid production. Autophagy-triggered lipid metabolism in microalgae has not being studied so far from a mutant of proton gradient regulation 1 like (PGRL1) and proton gradient regulation 5 (PGR5). In this study, C. reinhardtii cells (wild-type CC124 and cyclic electron transport dependant mutants pgrl1 and pgr5) were grown photoheterotrophically in high light 500 μmol photons m–2 s–1, where pgr5 growth was retarded due to an increase in reactive oxygen species (ROS). The lipid contents were increased; however, carbohydrate content was decreased in pgr5. Further, the Nile Red (NR) fluorescence shows many lipid bodies in pgr5 cells under high light. Similarly, the electron micrographs show that large vacuoles were formed in high light stress despite the grana stacks structure. We also observed increased production of reactive oxygen species, which could be one reason the cells underwent autophagy. Further, a significant increase of autophagy ATG8 and detections of ATG8-PE protein was noticed in pgr5, a hallmark characteristic for autophagy formation. Consequently, the triacylglycerol (TAG) content was increased due to diacylglycerol acyltransferases (DGAT) and phospholipid diacylglycerol acyl-transference (PDAT) enzymes’ expression, especially in pgr5. Here the TAG synthesis would have been obtained from degraded membrane lipids in pgr5. Additionally, mono, polyunsaturated, and saturated fatty acids were identified more in the high light condition. Our study shows that the increased light induces the reactive oxygen species, which leads to autophagy and TAG accumulation. Therefore, the enhanced accumulation of TAGs can be used as feedstock for biodiesel production and aqua feed.

Maximizing Photosynthesis-Driven Baeyer–Villiger Oxidation Efficiency in Recombinant Synechocystis sp. PCC6803

Photosynthesis-driven whole-cell biocatalysis has great potential to contribute to a sustainable bio-economy since phototrophic cells use light as the only energy source. It has yet to be shown that phototrophic microorganisms, such as cyanobacteria, can combine the supply of high heterologous enzyme levels with allocation of sufficient reduction equivalents to enable efficient light-driven redox biocatalysis. Here, we demonstrated that the heterologous expression of an NADPH-dependent Baeyer–Villiger monooxygenase (BVMO) gene from Acidovorax sp. CHX100 turns Synechocystis sp. PCC6803 into an efficient oxyfunctionalization biocatalyst, deriving electrons and O2 from photosynthetic water oxidation. Several expression systems were systematically tested, and a PnrsB-(Ni2+)–controlled expression based on a replicative plasmid yielded the highest intracellular enzyme concentration and activities of up to 60.9 ± 1.0 U gCDW−1. Detailed analysis of reaction parameters, side reactions, and biocatalyst durability revealed—on the one hand—a high in vivo BVMO activity in the range of 6 ± 2 U mgBVMO−1 and—on the other hand—an impairment of biocatalyst performance by product toxicity and by-product inhibition. Scale-up of the reaction to 2-L fed-batch photo-bioreactors resulted in the stabilization of the bioconversion over several hours with a maximal specific activity of 30.0 ± 0.3 U g CDW−1, a maximal volumetric productivity of 0.21 ± 0.1 gL−1 h−1, and the formation of 1.3 ± 0.1 gL−1 of ε-caprolactone. Process simulations based on determined kinetic data revealed that photosynthesis-driven cyclohexanone oxidation on a 2-L scale under high-light conditions was kinetically controlled and not subject to a limitation by photosynthesis.

Roles of Close Homologues SigB and SigD in Heat and High Light Acclimation of the Cyanobacterium Synechocystis sp. PCC 6803.

Acclimation of cyanobacterium Synechocystis sp. PCC6803 to suboptimal conditions is largely dependent on adjustments of gene expression, which is highly controlled by the σ factor subunits of RNA polymerase (RNAP). The SigB and SigD σ factors are close homologues. Here we show that the sigB and sigD genes are both induced in high light and heat stresses. Comparison of transcriptomes of the control strain (CS), ΔsigB, ΔsigD, ΔsigBCE (containing SigD as the only functional group 2 σ factor), and ΔsigCDE (SigB as the only functional group 2 σ factor) strains in standard, high light, and high temperature conditions revealed that the SigB and SigD factors regulate different sets of genes and SigB and SigD regulons are highly dependent on stress conditions. The SigB regulon is bigger than the SigD regulon at high temperature, whereas, in high light, the SigD regulon is bigger than the SigB regulon. Furthermore, our results show that favoring the SigB or SigD factor by deleting other group 2 σ factors does not lead to superior acclimation to high light or high temperature, indicating that all group 2 σ factors play roles in the acclimation processes.

Response of Posidonia oceanica (L.) Delile and Its Associated N2 Fixers to Different Combinations of Temperature and Light Levels

The increasing occurrence of heat waves and water turbidity are threats to the persistence of seagrass meadows. Their effects on the productivity of seagrasses and the functioning of their associated microorganisms have not been studied extensively. The purpose of this study is to assess the effects of different light levels and temperatures onPosidonia oceanica; the endemic seagrass species in the Mediterranean Sea, and their N2-fixing community, which contributes importantly to the nitrogen requirements and high productivity of the plants. Aquarium experiments were conducted in winter, when the plants are more vulnerable to changes in temperature, subjecting them to short-term exposures to an ambient (15.5°C) and elevated temperatures (ambient+5.5°C), and at limited (13 μmol photons m−2s−1) and saturating light conditions (124 μmol photons m−2s−1). Primary production, chlorophyll content, reactive oxygen species production, polyphenols content, thenifHgene expression, N2fixation, and alkaline phosphatase activities were measured in different plant tissues. Plants incubated at ambient temperature and high light exhibited an enhanced total chlorophyll production. Significantly higher gross and net primary production rates were also recorded under ambient temperature and high light conditions, which were approximately two-fold compared to the rest of the treatments. The oxidative stress analyses revealed an increased production of reactive oxygen species in young leaves incubated at ambient temperature and saturating light, while the polyphenols content in top leaves was considerably higher under elevated temperatures. In contrast, the N2fixation and alkaline phosphatase rates were significantly higher under elevated temperature and low light levels. The presence of the N2-fixing phylotypes UCYN-A, -B, and -C was detected through genetic analyses, with UCYN-B demonstrating the highestnifHgene transcription levels at elevated temperatures. These findings emphasize the significant role of irradiance on the productivity ofP. oceanicaand the temperature dependence of the N2fixation process in winter.

Net heterotrophy in High Arctic first-year and multi-year spring sea ice

The net productivity of sea ice is determined by the physical and geochemical characteristics of the ice–ocean system and the activity of organisms inhabiting the ice. Differences in habitat suitability between first-year and multi-year sea ice can affect the ice algal community composition and acclimation state, introducing considerable variability to primary production within each ice type. In this study, we characterized the biogeochemical variability between adjacent first-year and multi-year sea ice floes in the Lincoln Sea of the Canadian High Arctic, during the May 2018 Multidisciplinary Arctic Program—Last Ice sampling campaign. Combining measurements of transmitted irradiance from a remotely operated underwater vehicle with laboratory-based oxygen optode incubations, this work shows widespread heterotrophy (net oxygen uptake) in the bottom 10 cm of both ice types, particularly in thick multi-year ice (>2.4 m) and early morning of the 24-h day. Algal acclimation state and species composition varied between ice types despite similar net community production due to widespread light and nutrient limitation. The first-year ice algal community was increasingly dominated over spring by the potentially toxin-producing genus Pseudonitzschia that was acclimated to high and variable light conditions characteristic of a thinner ice habitat with mobile snow cover. In comparison, the multi-year ice harbored more shade-acclimated algae of mixed composition. This work highlights the potential for heterotrophy in sea ice habitats of the High Arctic, including first measurements of such O2-uptake in multi-year ice floes. Observed differences in photophysiology between algae of these sea ice types suggests that a shift toward higher light availability and a younger sea ice cover with climate change does not necessarily result in a more productive system. Instead, it may favor future sea ice algal communities of different species composition, with lower photosynthetic potential but greater resilience to stronger and more variable light conditions.

Differential ecophysiological responses to inorganic nitrogen sources (ammonium versus nitrate) and light levels in the seagrass Zostera noltei

Seagrasses can use both ammonium (NH4+) and nitrate (NO3-) as inorganic nitrogen (N) sources. However, NO3- uptake and assimilation are energetically more expensive and tightly regulated than NH4+ uptake. The objective of this study was to test the complex interactive effects between different forms of N enrichment (NH4+ and NO3-) and light levels on the morphological and physiological traits in the intertidal seagrass Zostera noltei. Plants were cultured over 40 d under 2 levels of light (low and high) with 2 inorganic N concentrations supplied at the same dose, NO3- (25 µM) and NH4+ (25 µM), and a control, following a 2-factorial design. Results showed a differential response in Z. noltei depending on the inorganic N source and light dose. NH4+ enrichment negatively affected almost all morphometric and dynamic variables analyzed, both in isolation and combined with low light conditions. In contrast, NO3- enrichment had a positive effect on Z. noltei survival compared with the control treatment in terms of net growth rate and rhizomatic growth, mainly under high light conditions. Therefore, our study demonstrated that the effects promoted by nutrient enrichment largely depend on the source of N used. Light levels play a crucial role in this response by potentially shifting the effects from toxic (under low light) to beneficial (under high light) when NO3- is the main N source. Our findings highlight that N form in eutrophication events should be considered when evaluating the potential impacts of nutrient enrichment and light reduction on seagrass communities.

Analysis of a mechanistic model of corals in association with multiple symbionts: within-host competition and recovery from bleaching.

Coral reefs are increasingly experiencing stressful conditions, such as high temperatures, that cause corals to undergo bleaching, a process where they lose their photosynthetic algal symbionts. Bleaching threatens both corals’ survival and the health of the reef ecosystems they create. One possible mechanism for corals to resist bleaching is through association with stress-tolerant symbionts, which are resistant to bleaching but may be worse partners in mild conditions. Some corals have been found to associate with multiple symbiont species simultaneously, which potentially gives them access to the benefits of both stress-sensitive and -tolerant symbionts. However, within-host competition between symbionts may lead to competitive exclusion of one partner, and the consequences of associating with multiple partners simultaneously are not well understood. We modify a mechanistic model of coral-algal symbiosis to investigate the effect of environmental conditions on within-host competitive dynamics between stress-sensitive and -tolerant symbionts and the effect of access to a tolerant symbiont on the dynamics of recovery from bleaching. We found that the addition of a tolerant symbiont can increase host survival and recovery from bleaching in high-light conditions. Competitive exclusion of the tolerant symbiont occurred slowly at intermediate light levels. Interestingly, there were some cases of post-bleaching competitive exclusion after the tolerant symbiont had helped the host recover.

Effects of cultivation conditions on biofortification of yogurt with natural folate by Propionibacterium freudenreichii

In this study, Propionibacterium (P.) freudenreichii was used for the folate production by co-culturing with traditional yogurt starter cultures in synbiotic yogurt and then it was compared to control yogurt containing only LAB starters. First, effects of process variables, including two probiotic strains, inoculum size, inulin concentration, milk fat percentage, sunflower oil content, fermentation temperature and storage time of incubation at 4 °C on folate production by P. freudenreichii were investigated using screening method of Plackett-Burman design. Then, optimization of folate production process was carried out using three main effects of inulin concentration, fermentation temperature and storage time at 4 °C using central composite design. Analysis of variance showed that the models were highly significant (p ≤ 0.05) and folate production affected by the three factors. Optimized folate production by P. freudenreichii ssp. shermanii in yogurts was achieved after 12 d of storage at 4 °C in 2% (w/v) skim-milk inulin as prebiotic and fermentation temperature of 38.6 °C. Reconfirmation assays showed that the highest produced folate included 39.2 ± 0.7 μg 100 mL-1 in FW yogurt containing P. freudenreichii ssp. shermanii under the highlighted optimum conditions, which increased by 49% of the total folate production, compared to control yogurt, which contained only LAB starters. Furthermore, textural properties were assessed using texture analysis. Coculture of P. freudenreichii with traditional yogurt starter culture and addition of inulin to yogurt samples increased hardness, consistency and cohesiveness values in yogurt samples. Results showed that by co-culturing of P. freudenreichii ssp. shermanii in yogurt, in addition to producing a probiotic product, also increases the amount of folate production compared to control yogurt, which contained only LAB starters.

The Aspects of Sustainable Development in Mineral Resources Management

Mineral resources provide a high standard of living for modern societies: satisfying electricity demand as well as demand for construction materials and they sustain the basis for industry and technological development. Now, modern societies are facing the challenge ofreversing the trend of the depletion of non-renewable mineral resources and sustainable development is intended to ensure the survivalof human civilization in the face of dwindling non-renewable raw materials (especially energy resources) and also increasing anthropopression and related environmental pollution. The amount of non-renewable mineral resources of the Earth's crust is limited. Underspecific conditions there is a possibility of their regeneration however over a period of several if not more than a dozen generations. Thearticle raises questions how societies can prevent mineral resources crises in future and whether this task is feasible.The article identifies the main aspects of the sustainable development in mining sector in Poland as well as environmental challengesrelated to the new CSR mechanism which are: the creation and implementation of sustainable and responsible business model whichthanks to reformed financial and economic system, will make creating a better future easier, more natural and more cost-effective.The crucial aspects of sustainable development as economic and social conditions, environmental challenges, safety of agglomerationlocated in the area of exploitation of resources or in its neighborhood were also presented in the article. The author highlighted thelegal conditions for the management of deposits and extracted mineral resources as well as work safety and research and developmentactivities in the sector.

Improvement of Accuracy and Precision of the LiDAR System Working in High Background Light Conditions

Background light noise is one of the major challenges in the design of Light Detection and Ranging (LiDAR) systems. In this paper, we build a single-beam LiDAR module to investigate the effect of light intensity on the accuracy/precision and success rate of measurements in environments with strong background noises. The proposed LiDAR system includes the laser signal emitter and receiver system, the signal processing embedded platform, and the computer for remote control. In this study, two well-known time-of-flight (ToF) estimation methods, which are peak detection and cross-correlation (CC), were applied and compared. In the meanwhile, we exploited the cross-correlation technique combined with the reduced parabolic interpolation (CCP) algorithm to improve the accuracy and precision of the LiDAR system, with the analog-to-digital converter (ADC) having a limited resolution of 125 mega samples per second (Msps). The results show that the CC and CCP methods achieved a higher success rate than the peak method, which is 12.3% in the case of applying emitted pulses 10 µs/frame and 8.6% with 20 µs/frame. In addition, the CCP method has the highest accuracy/precision in the three methods reaching 7.4 cm/10 cm and has a significant improvement over the ADC’s resolution of 1.2 m. This work shows our contribution in building a LiDAR system with low cost and high performance, accuracy, and precision.

Function of Chloroplasts in Plant Stress Responses.

The chloroplast has a central position in oxygenic photosynthesis and primary metabolism. In addition to these functions, the chloroplast has recently emerged as a pivotal regulator of plant responses to abiotic and biotic stress conditions. Chloroplasts have their own independent genomes and gene-expression machinery and synthesize phytohormones and a diverse range of secondary metabolites, a significant portion of which contribute the plant response to adverse conditions. Furthermore, chloroplasts communicate with the nucleus through retrograde signaling, for instance, reactive oxygen signaling. All of the above facilitate the chloroplast’s exquisite flexibility in responding to environmental stresses. In this review, we summarize recent findings on the involvement of chloroplasts in plant regulatory responses to various abiotic and biotic stresses including heat, chilling, salinity, drought, high light environmental stress conditions, and pathogen invasions. This review will enrich the better understanding of interactions between chloroplast and environmental stresses, and will lay the foundation for genetically enhancing plant-stress acclimatization.

Transcriptome Analysis Reveals Roles of Anthocyanin- and Jasmonic Acid-Biosynthetic Pathways in Rapeseed in Response to High Light Stress.

Rapeseed (Brassica napus) is one of the major important oil crops worldwide and is largely cultivated in the Qinghai-Tibetan plateau (QTP), where long and strong solar-radiation is well-known. However, the molecular mechanisms underlying rapeseed’s response to light stress are largely unknown. In the present study, the color of rapeseed seedlings changed from green to purple under high light (HL) stress conditions. Therefore, changes in anthocyanin metabolism and the transcriptome of rapeseed seedlings cultured under normal light (NL) and HL conditions were analyzed to dissect how rapeseed responds to HL at the molecular level. Results indicated that the contents of anthocyanins, especially glucosides of cyanidin, delphinidin, and petunidin, which were determined by liquid chromatography-mass spectrometry (LC-MS), increased by 9.6-, 4.2-, and 59.7-fold in rapeseed seedlings exposed to HL conditions, respectively. Next, RNA-sequencing analysis identified 7390 differentially expressed genes (DEGs), which included 4393 up-regulated and 2997 down-regulated genes. Among the up-regulated genes, many genes related to the anthocyanin-biosynthetic pathway were enriched. For example, genes encoding dihydroflavonol reductase (BnDFR) and anthocyanin synthase (BnANS) were especially induced by HL conditions, which was also confirmed by RT-qPCR analysis. In addition, two PRODUCTION OF ANTHOCYANIN PIGMENTATION 2 (BnPAP2) and GLABRA 3 (BnGL3) genes encoding MYB-type and bHLH-type transcription factors, respectively, whose expression was also up-regulated by HL stress, were found to be associated with the changes in anthocyanin biosynthesis. Many genes involved in the jasmonic acid (JA)-biosynthetic pathway were also up-regulated under HL conditions. This finding, which is in agreement with the well-known positive regulatory role of JA in anthocyanin biosynthesis, suggests that the JA may also play a key role in the responses of rapeseed seedlings to HL. Collectively, these data indicate that anthocyanin biosynthesis-related and JA biosynthesis-related pathways mediate HL responses in rapeseed. These findings collectively provide mechanistic insights into the mechanisms involved in the response of rapeseed to HL stress, and the identified key genes may potentially be used to improve HL tolerance of rapeseed cultivars through genetic engineering or breeding strategies.

Vertical Distribution of Phytoplankton Community and Pigment Production in the Yellow Sea and the East China Sea during the Late Summer Season

Phytoplankton community structure, which plays an important role in determining productivity and food web structure, can provide important information for understanding variations in marine ecosystems under projected climate change scenarios. Rising temperatures due to climate change will increase and intensify water stratification. To understand the community composition and distribution characteristics of phytoplankton under stratified conditions, phytoplankton pigments were analyzed in the Yellow Sea (YS) and East China Sea (ECS) during the late summer season. In addition, pigment production was measured to estimate the physiological characteristics of phytoplankton relating to light, which is an essential element of photosynthesis. During our observation period, no distinct differences were found in the community composition and pigment production of phytoplankton in the YS and the ECS, but differences in the vertical distribution were observed. Overall, the dominant phytoplankton classes at the surface depth were pico-sized cyanobacteria (46.1%), whereas micro- and nano-sized diatoms (42.9%) were the abundant most classes at a 1% light depth. The major factors controlling the vertical distributions of the phytoplankton community were temperature and nutrients (i.e., nitrate and ammonium). Cyanobacteria were positively correlated with water temperature and ammonium, whereas diatoms were negatively related to water temperature and positively correlated with nitrates. Based on the pigment production, it was found that cyanobacteria at the surface layer encountered excessive irradiance conditions during the study period. The productivity of the cyanobacterial community could be decreased under high-light and high-temperature conditions. This means that cyanobacteria could have a negative influence on the quantity and quality of food available to upper trophic organisms under warmer conditions.

Comparative Physiological, Biochemical, and Proteomic Responses of Photooxidation-Prone Rice Mutant 812HS under High Light Conditions

Photosynthetic efficiency decreases as light energy surpasses the photosynthesis capacity. This study was designed to investigate the potential effects of high-intensity light on the photooxidation-prone mutant 812HS of rice and its wild-type 812S during yellow and recovering stages. Results showed that in the yellowing stage, light oxidation occurs due to the exposure of mutant 812HS leaves to the high sunlight, which causes yellowing of the leaves, leading to a reduction in the photochemical activities, physiological mechanisms, and protein contents in mutant 812HS. In the recovery stage, mutant 812HS leaves were exposed to the maximum high brightness, the mutant’s leaves were draped with a dark cover to decrease the exposure of leaves of the plants from direct sunlight, which leads to the restoration of the green color again to the mutant 812HS leaves, leading to improving the performance of the photochemical activities, physiological mechanisms, and protein contents in mutant 812HS. Exposing leaves of mutant 812HS to high light at the yellow stage also resulted in a decrease in the net photosynthetic rate (Pn) in carotenoids content and chlorophyll a and b. Similarly, chlorophyll fluorescence of mutant 812HS decreased in (O-I-J-I-P) curves, and the ATP content, Mg2+-ATPase, and Ca2+-ATPase activities also decreased. An increase in energy dissipation was observed, while ABS/RC, DI0/RC, and TR0/RC values in mutant 812HS at the yellow stage increased. During photooxidation, an increase in O2•– and H2O2 contents was observed in mutant 812HS. While O2•– and H2O2 contents were decreased in mutant 812HS at the recovery stage. The rate of thylakoid membrane protein content was significantly decreased in mutant 812HS at the yellow stage, while at the recovery stage, there was no significant decrease. Our findings showed that photooxidation prompted oxidative damages and lipid peroxidation that caused severe damages to the membranes of the cell, photosynthetic pigments degradation, protein levels, and photosynthesis inhibition in mutant 812HS.

Interactive effects of light, CO2 and temperature on growth and resource partitioning by the mixotrophic dinoflagellate, Karlodinium veneficum.

There is little information on the impacts of climate change on resource partitioning for mixotrophic phytoplankton. Here, we investigated the hypothesis that light interacts with temperature and CO2 to affect changes in growth and cellular carbon and nitrogen content of the mixotrophic dinoflagellate, Karlodinium veneficum, with increasing cellular carbon and nitrogen content under low light conditions and increased growth under high light conditions. Using a multifactorial design, the interactive effects of light, temperature and CO2 were investigated on K. veneficum at ambient temperature and CO2 levels (25°C, 375 ppm), high temperature (30°C, 375 ppm CO2), high CO2 (30°C, 750 ppm CO2), or a combination of both high temperature and CO2 (30°C, 750 ppm CO2) at low light intensities (LL: 70 μmol photons m-2 s-2) and light-saturated conditions (HL: 140 μmol photons m-2 s-2). Results revealed significant interactions between light and temperature for all parameters. Growth rates were not significantly different among LL treatments, but increased significantly with temperature or a combination of elevated temperature and CO2 under HL compared to ambient conditions. Particulate carbon and nitrogen content increased in response to temperature or a combination of elevated temperature and CO2 under LL conditions, but significantly decreased in HL cultures exposed to elevated temperature and/or CO2 compared to ambient conditions at HL. Significant increases in C:N ratios were observed only in the combined treatment under LL, suggesting a synergistic effect of temperature and CO2 on carbon assimilation, while increases in C:N under HL were driven only by an increase in CO2. Results indicate light-driven variations in growth and nutrient acquisition strategies for K. veneficum that may benefit this species under anticipated climate change conditions (elevated light, temperature and pCO2) while also affecting trophic transfer efficiency during blooms of this species.