High Levels Of Carbohydrates Research Articles

Comparative Study of Proximate and Mineral Composition of Cocoyam (Cococasia Esculenta) Grown From Bauchi, Plateau, Gombe and Kaduna States of Northern Nigeria

The proximate and mineral composition of cocoyam (colocasiaescienta) grown in Bauchi, Plateau, Gombe and Kaduna States were analyzed using association of official analytical chemistry. The analysis included, ash; 3%, 2.6% and 3.2% crude protein; 2.7%, 3.4%, 2.8% and 2.9% crude fibre; 1.5%, 1.9%, 1.8% and 1.0% crude fat; 0.3%, 0.3%, 0.2% and 0.4%. Moisture; 19%, 21%, 15.3% and 16.5%. Carbohydrate; 73.5%, 70.5%, 77.3% and 75.6% for the results obtained from Bauchi, Plateau, Gombe and Kaduna States. The mineral contents were determined using Atomic Absorption spectrophotometer (ADS), were as follows; P. 51423 PPM, 803.11PPM, 550.71PPM and 445.45PPM. Zn: 7.53PPm, 14.01PPM, 10.25PPM, 7.4PPM. Ca: 896.1PPM, 1110.39PPM, 864.98PPM and 884.87PPM. Fe: 48.91PPM, 3.21PPM, 50.88PPM and 31.67PPM. Cu: 6.58PPM, 11.09PPM, 4.86PPM and 5.9PPM. K: 3478.71PPM, 4630.20PPM, 3617.91PPM and 3236.63PPM. Mg: 334.11PPM, 506.73PPM, 367.73PPM and 324.71PPM. Mn: 5.11PPM, 8.43PPM, 6.30PPM and 4.80PPM. Na: 204.89PPM, 203.59PPM, 159.34PPM and 191.12PPM for the results of samples obtained from Bauchi, Plateau, Gombe and Kaduna States, the high level of carbohydrate observed from all the samples also agrees with the finding reported by FOA (1990), that the main nutrients supplied is dietary energy provided by the carbohydrates. The results from the current study reveal that cocoyam is nutritionally rich in carbohydrate and minerals such as potassium and sodium.

Developing Countries' Challenges in Cultivating Salt-Tolerant Fodder Peas for Animal Feed

As a result of the rapid increase in the world population, the problem of balanced and adequate nutrition has emerged, and the importance of animal production has increased even more. In this respect, to ensure sufficient and balanced nutrition of existing animals, there is a need to increase the cultivation areas of fodder crops and proper management of pastures and breeding studies. For this reason, fodder pea (Pisum sativum L.), which has high nutritional value and is preferred for animal consumption, is a vital fodder plant to meet this need. Fodder peas are suitable for consumption as animal feed because they contain high levels of carbohydrates and digestible substances. However, salinity, one of the abiotic stress factors, is an essential problem for fodder peas. Salinity significantly limits the development of the plant and leads to yield losses. Although the consequences of climate change vary in many parts of the world, it is predicted that the frequency and severity of conditions such as decreased precipitation, increased temperatures, floods, droughts, and increased saline areas will increase with climate change in developing countries. In addition, salt stress also affects the photosynthetic mechanism in plants and causes changes in chlorophyll, carotenoid, phenolic, and antioxidant concentrations. Since the reclamation of saline farmland is expensive and complex, it is more appropriate to grow salinity-resistant plants. Therefore, gene studies to improve the salinity tolerance of plants have gained momentum in recent years. In this review, studies conducted in the last 20 years are discussed. Salt tolerance in gene-transferred and non-transferred peas, as well as plant growth in a saline environment, were assessed by comparing proline, chlorophyll, total phenolic, and antioxidant levels. In summary, this study seeks to highlight the issues of drought, aridity, and salinity, all of which are expected to worsen as climate change progresses.

Discrimination of superficial lymph nodes using ultrasonography and tissue metabolomics coupled with machine learning.

Diagnosing the types of malignant lymphoma could help determine the most suitable treatment, anticipate the probability of recurrence and guide long-term monitoring and follow-up care. We evaluated the differences in benign, lymphoma and metastasis superficial lymph nodes using ultrasonography and tissue metabolomics. Our findings indicated that three ultrasonographic features, blood supply pattern, cortical echo, and cortex elasticity, hold potential in differentiating malignant lymph nodes from benign ones, and the shape and corticomedullary boundary emerged as significant indicators for distinguishing between metastatic and lymphoma groups. Metabolomics revealed the difference in metabolic profiles among lymph nodes. We observed significant increases in many amino acids, organic acids, lipids, and nucleosides in both lymphoma and metastasis groups, compared to the benign group. Specifically, the lymphoma group exhibited higher levels of nucleotides (inosine monophosphate and adenosine diphosphate) as well as glutamic acid, and the metastasis group was characterized by higher levels of carbohydrates, acylcarnitines, glycerophospholipids, and uric acid. Linear discriminant analysis coupled with these metabolites could be used for differentiating lymph nodes, achieving recognition rates ranging from 87.4% to 89.3%, outperforming ultrasonography (63.1% to 75.4%). Our findings could contribute to a better understanding of malignant lymph node development and provide novel targets for therapeutic interventions.

Antitumor activity of edible fishes (Channa striata and Anabas testudineus) and gastropods (Helix aspersa and Pila virens) rudimentary mucus against HT-29 cell line and its biochemical properties

BackgroundThe mucus from fish and gastropods contains a wide range of bioactive molecules with biomedical properties. The fish and gastropods were collected from Oragadam lake, Kanchipuram district, Tamil Nadu, India. In this study, we wanted to examine the anticancer potential of fish and gastropods mucus. The biochemical components of the crude mucus were screened.ResultsThe biochemical analysis showed that the mucus of Anabas testudineus and Pila virens contained a high level of carbohydrates (2.8 and 1.5 mg/ml), the mucus of Channa striata contained a high level of lipids (0.9 mg/ml), and the mucus of Helix aspersa contained a high level of protein (1.3 mg/ml). The results showed morphological variations in the HT-29 cells upon treatment with crude mucus. Upon 24 h of gestation, the frozen cells began to shrink and seem round in shape. Using the MTT assay, the mucus crude extract was evaluated for its anticancer properties against the human colon cancer cell line (HT 29). The inhibitory concentration (IC50) was determined at 100 µg/ml after 24 h. Using specific staining techniques; fluorescent microscopy was utilized to examine the cell morphology and early and late apoptotic stages. Propidium iodide staining showed nuclear damage followed by DNA damage. This showed that the rudimentary mucus could prompt cell death and increased the number of fragments and mucus concentration, respectively.ConclusionThis study showed that the edible or commercially important fish and gastropod mucus have potential anticancer activity against HT-29 cancer cells.

Effects of Different Carbohydrate Levels in Diets on Growth Performance and Muscle Nutritive Value of Ying Carp and Scattered-Scaled Mirror Carp (Cyprinus carpio).

This experiment aimed to assess the dietary adaptation and utilization of high carbohydrate diets to Ying carp and scattered-scaled mirror carp (Cyprinus carpio), focusing on growth performance, muscle nutritive value, and nutrient metabolism. Ying carp (4.5 ± 0.2 g) and scattered-scaled mirror carp (5.01 ± 0.2 g) were fed isonitrogenous diets containing 20%, 30%, and 40% carbohydrates for 8 weeks; the nitrogen content of the three feeds was (5.12% ± 0.03%). After the trail, the final body weight, feed efficiency, and specific growth rate of both carp varieties were analyzed. Results showed that the final body weight, feed efficiency, and specific growth rate of both carp varieties were significantly higher at the 30% carbohydrate level compared to 20% and 40%, indicating improved growth performance (p < 0.05). Crude protein content in whole fish composition was significantly higher at the 30% carbohydrate level compared to the other two levels (p < 0.05) for both varieties. However, excessive carbohydrate intake (40%) led to pronounced liver fat deposition in both varieties, with scattered-scaled mirror carp showing less severe deposition than Ying carp. As the carbohydrate levels in the feed increased, the essential amino acid (EAA) and total amino acid (TAA) content in the muscle of both carp varieties significantly increased, while the content of unsaturated fatty acids in the muscle significantly decreased (p < 0.05). Gene expression analysis revealed enhanced glycolytic activity (pk1) and inhibited gluconeogenesis (g6p and pepck) in the liver with higher carbohydrate levels. In muscle tissue, high carbohydrate diets reduced expression levels of genes involved in polyunsaturated fatty acid (PUFA) synthesis (elovl5, elovl6, and rxrgb). The two carp varieties exhibited distinct adaptations to varying dietary carbohydrate levels. While a 30% carbohydrate diet enhanced the growth performance of both varieties, scattered-scaled mirror carp demonstrated higher growth efficiency, whereas Ying carp excelled in maintaining muscle nutrient quality, particularly regarding PUFAs and amino acid composition. These findings suggest that carbohydrate levels in feed should be optimized based on the specific goals of aquaculture, whether prioritizing rapid growth or improved muscle nutrition. Moreover, variations in the expression of genes related to carbohydrate metabolism between the two varieties influenced their metabolic responses, offering insights for designing variety-specific feeding strategies to support sustainable aquaculture practices.

Biochemical Characterization and Antimicrobial Properties of Extracts From Four Food Plants Traditionally Used to Improve Drinking Water Quality in Rural Areas of Burkina Faso.

The use of local plant extracts in drinking water purification represents a sustainable alternative in the fight against diseases linked to unsafe water consumption in rural areas. The aim of this study was to evaluate the biochemical composition and antimicrobial activity of four local plant extracts used in rural areas of Burkina Faso to purify drinking water: Moringa oleifera seeds, Boscia senegalensis seeds, Opuntia ficus-indica cladodes, and Aloe vera leaves. These four extracts were then subjected to biochemical screening to identify phytocompounds, followed by quantification and evaluation of their antibacterial properties on ten pathogenic bacterial strains. The screening results revealed the presence of a variety of molecules (phenolic compounds, alkaloids, saponosides, etc.) in the different extracts studied. From a quantitative point of view, M. oleifera and B. senegalensis seeds showed a high total protein content (34.5 and 24.6 g/100 g DM). A. vera and O. ficus-indica extracts showed high levels of total carbohydrates (20.4 and 35.52 g/100 g DM) compared with total lipids and proteins. The same applies to phenolic compounds, which were also high in A. vera and O. ficus-indica extracts (17.42 and 26.5 mg GAE/100 mg DM) compared to M. oleifera and B. senegalensis seeds. In terms of antibacterial properties, the four extracts studied showed inhibition diameters ranging from 7.33 to 16.33 mm. These results reflect the ability of the different extracts to eliminate pathogenic microorganisms present in water. Overall, this study stands out for its innovative character, offering an in-depth understanding of the biochemical composition and antimicrobial properties of four distinct extracts of local plants commonly used in traditional drinking water purification practices. It enriches existing knowledge by providing new data on the biochemical composition and bioactivity of these extracts. In particular, the study highlights the synergistic effects of the bioactive compounds present, underlining their essential role in improving the sanitary quality of water consumed in rural areas, where sustainable and accessible solutions are crucial.

A Comprehensive Analysis of the Nutritional Value, Antioxidant Potential and Fatty Acid Composition of Lucuma (Pouteria lucuma) Fruit, Grown in the High-Altitude Valleys of Bolivia.

The lucuma tree (Pouteria lucuma), native to South America, is gaining attention for its unique nutritional profile and potential health benefits. This study aimed to analyze the nutritional composition, antioxidant capacity, and fatty acid profile of lucuma fruit from a high-altitude valley in Bolivia. The proximate analysis revealed high levels of carbohydrates (41.7%), dietary fibre (2.4%), and protein (6.9%). Antioxidant assays identified significant amounts of polyphenols, flavonoids, and carotenoids, known for their antioxidant and anti-inflammatory properties. The fruit's fatty acid profile showed a healthy omega-6:omega-3 ratio of 0.21. These results highlight lucuma's potential as a functional food and support further research into its health benefits.

Postharvest Quality of Calcium Chloride-Treated Strawberry (Fragaria x ananassa cv. Festival) in CoolBot Storage

Though strawberry is a popular and nutritious fruit gladly accepted worldwide, its delicate nature and high perishability makes it highly susceptible to postharvest loss. The objective of this study was to evaluate the postharvest quality attributes including shelf life of ‘Festival’ strawberry as affected by calcium chloride in CoolBot storage system. The experiment was conducted with four calcium chloride concentrations [control (no calcium), 1%, 2%, and 3% calcium chloride] following Completely Randomized Design with three replications. Each replication consisted of 400 g freshly harvested strawberry fruits which were dipped into the respective calcium chloride solutions for two minutes followed by 10-minutes air drying. The findings showed that 3% calcium chloride resulted in the highest anthocyanin content (40.62 mg 100g-1) and shelf life (10.33 days) along with the lowest fruit weight loss (11.18%). The 3% calcium chloride also maintained the higher fruit diameters (polar and equatorial) and firmness along with high level of titratable acidity, vitamin C, and total carbohydrate and low level of total soluble solids compared to the control. However, the lowest fruit spoilage (11.59%) was reported from 2% calcium chloride. Overall, 3% calcium chloride performed better regarding physical and chemical quality attributes and shelf life of strawberry at postharvest and therefore, it can be suggested for postharvest strawberry fruit preservation. However, further research could be conducted with other strawberry varieties to have a better understanding of postharvest strawberry preservations with calcium chloride in the CoolBot storage facilities. J Bangladesh Agril Univ 22(4): 547-557, 2024

Effects of dietary carbohydrate and lipid levels on growth performance, hepatic histology and antioxidant capacity and flesh texture of mandarin fish (Siniperca chuatsi).

In this study, nine isonitrogenous experimental diets containing graded levels of carbohydrates (40 g/kg, 80 g/kg and 120 g/kg) and crude lipids (80 g/kg, 120 g/kg and 160 g/kg) were formulated in a two-factor (3 × 3) orthogonal design. A total of 945 mandarin fish with similar body weights were randomly assigned to twenty-seven tanks, and the experiment diets were fed to triplicate tanks twice daily for 10 weeks. Results showed that different dietary treatments did not significantly affect the survival rate and growth performance of mandarin fish. However, high dietary lipid and carbohydrate levels significantly decreased the protein content of the whole body and muscle of cultured fish. The lipid content of the whole body, liver and muscle all significantly increased with increasing levels of dietary lipid, while only liver lipid level was significantly affected by dietary carbohydrate level. Hepatic glycogen content increased significantly with increasing dietary carbohydrate levels. As to liver antioxidant capacity, malondialdehyde content increased significantly with increasing dietary lipid or carbohydrate content, and catalase activity showed an opposite trend. Superoxide dismutase activity increased significantly with increasing levels of dietary lipid but decreased first and then increased with increasing dietary carbohydrate levels. Additionally, the increase in both dietary lipid and carbohydrate levels resulted in a significant reduction in muscle hardness. Muscle chewiness, gumminess and shear force were only affected by dietary lipid levels and decreased significantly with increasing dietary lipid levels. In conclusion, considering all the results, the appropriate dietary lipids and carbohydrate levels for mandarin fish were 120 g/kg and 80 g/kg, respectively.

Proximate Analysis of Lemongrass Powder (Cymbopogon citratus)

Lemongrass (Cymbopogon citratus), a widely utilized aromatic herb, holds significant potential as a nutritional and functional food ingredient. This study evaluates the proximate composition of lemongrass powder to determine its key nutritional components, including moisture, ash, crude protein, crude fat, crude fibre, and carbohydrates. The results reveal a low moisture content (6.5 ± 0.2%), indicative of prolonged shelf life, and moderate ash content (4.3 ± 0.1%), suggesting a rich mineral profile. The crude protein (8.2 ± 0.3%) and fat (3.1 ± 0.2%) contents highlight its modest contribution to dietary protein and low-fat applications. Notably, the high crude fibre (16.7 ± 0.4%) and carbohydrate (61.2 ± 0.5%) levels underline its suitability as a dietary fibre source and energy provider. These findings support lemongrass powder's utility in food formulations and nutraceuticals, emphasizing its potential health benefits and multifunctionality. Further research on its bioactive compounds and antioxidant properties is recommended to expand its applications.

Biosurfactant Producing Bacteria from Groundnut Oil Cake and its Application in Pesticide Removal

A member of the class of organophosphate pesticides that are commonly used in agricultural activities worldwide is ethion, also known by its chemical name, phosphorodithioate, or O, O, O’, O’-tetraethyl S, S’-methylene bis. Its broad-spectrum insecticidal properties make it indispensable for controlling various pests in crops such as fruits, vegetables, and cereals. However, the indiscriminate use of ethion raises concerns due to its toxicity to non-target organisms, persistence in the environment, and potential adverse effects on human health. It was studied how bacteria that produce biosurfactants and were isolated from groundnut oil cake broke down ethion aerobically. In comparison to the FCO limitations of 5.77, 1.05, and 2.43 for Nitrogen, Phosphorus, and Potassium, the chemical analysis of groundnut oil cake revealed greater levels of these elements. Based on nutritional analysis, groundnut cake has low fiber content but high levels of carbohydrates (34.3%), moisture, starch, and proteins (5.33%, 16.7% and 36.12%, respectively), fat (13.67%), and ash (5.42%). The results of biosurfactant production screening showed that 17 isolates were positive for haemolytic activity, 11 were positive for Drop collapsed test and 2 isolates B. niabensis (B) and B. endophyticus (C) showed highest emulsification index 80% and 82% respectively. These two isolates B. niabensis and B. endophyticus were identified by 16S rRNA sequencing method. Both of them were found to produce glycolipid and lipopeptide after FTIR analysis. GC-MS analysis of degradation study showed significant degradation of ethion. The findings have consequences for how a bioremediation approach should be developed.

Baobab (Adansonia digitata L.) as a remedy to human livelihood challenges: a review

Baobab (Adansonia digitata L.) is a unique plant predominant in different ecosystems in Africa. This review assessed its value in relation to human nutrition, health, and related aspects. Our synthesis shows that baobab leaves, seed oil, and fruit pulp were utilised in different forms. Baobab tree products such as fruit pulp, leaves, and seed oil contain high levels of macronutrients, micronutrients, vitamins, carbohydrates, and proteins. Therefore, promoting the utilization and processing of baobab products for improved human nutrition is key. However, the determination of optimal parameters through elemental traceability as well as the relationship between geographical locations and nutrient composition of the baobab plant is required. Altogether, promoting innovative utilization of baobab products by exploring processing techniques to develop novel food and product formulation from Adansonia digitata tree products sheds a green light to sustain human livelihoods.

Nutritional, Antioxidant, and Functional Properties of Cameroonian Cowpea and Bambara Groundnut and Their Culinary Forms.

Background Voandzou and cowpea are two legumes commonly used in African dishes as alternative sources of proteins. The present study aimed to evaluate the effects of steaming and frying on the nutritional and functional properties of Cameroonian cowpea (Vigna unguiculata) and Bambara groundnut (Vigna subterranea). Methodology The nutritional values, as well as the antioxidant, alpha-amylase, and alpha-glucosidase inhibitory activities of "koki" and "beignet koki," which are the traditional steamed dish and fritters made from cowpea and Bambara groundnut, respectively, were assessed. Results All the legumes displayed high levels of lipids, proteins, and carbohydrates. It was observed that both steaming and frying increased the lipid content and the energy values of cowpea and Bambara groundnut while simultaneously reducing the protein and carbohydrate contents. Both raw and processed legumes contained varying amounts of the minerals examined, with the treatments having inconsistent effects on them. The total phenolic content (TPC) and total flavonoid content (TFC) were significant in both legumes, with cowpea exhibiting the highest levels (TPC of 676.46 ± 16.87 µg gallic acid equivalents (GAE) per gram of extract and TFC of 219.27 ± 4.62 mg quercetin equivalents (QE) per gram of raw brown and black-eyed cowpeas, respectively). The antioxidant capacities, measured using different methods, were negatively impacted by both steaming and frying due to the destruction of their bioactive compounds. The alpha-amylase and alpha-glucosidase inhibitory capacities of both legumes experienced similar reductions after culinary treatments. Conclusions Cowpea and Bambara groundnuts are valuable sources of proteins, energy, and antinutrients. They are sufficiently rich in phenolic compounds with antioxidant and inhibitory effects on alpha-amylase and alpha-glucosidase. Consequently, these legumes can be used as functional foods. Stifled cooking and frying reduce mineral content (except for sodium), bioactive compound levels, and antioxidant, alpha-amylase, and alpha-glucosidase inhibitory capacities of the studied legumes.

Evaluation of Nutritional Composition of Ocimum gratissimum Leaf Extract

Vegetables are known to be vital sources of nutritive foods. This study evaluates the nutritional composition of Ocimum gratissimum leaf extract vis-a-vis proximate, minerals and vitamins analyses. Fresh leaves of O. gratissimum were purchased from a local market in Awka North Local Government Area of Anambra State, Nigeria and were washed, destalked, oven-dried at 40 0C, pulverized, and sieved through 2.0 mm mesh prior to analysis. Proximate, minerals, and vitamin analyses were carried out, using standard methods. Results indicated that O. gratissimum contained high levels of carbohydrate (51.78 ± 0.50 %), protein (10.50 ± 0.04 %), and fiber (9.38 ± 0.05 %), with minute quantity of crude fat (4.94 ± 0.03 %). Analysis of the mineral compositions showed that the vegetable contained considerable quantity of minerals that is sufficient to improve general well-being, while the vitamin analysis revealed that the leaves of O. gratissimum was sufficiently rich in Vitamin E and could, therefore, act as antioxidant and consequently help to improve health status of its consumers.

Drones and Workers of Honeybee Apis mellifera L. Dried Powder: Chemical Composition, Antioxidant, and Anticancer Assessment.

This study explores honeybee as a food source through chemical analysis of pupa and adult stages of honeybee drones and workers (Apis mellifera L.). The findings reveal that drones exhibited higher protein and fat content, while workers have the highest carbohydrate levels. Additionally, seventeen amino acids and nine minerals were identified, with drones in the adult stage having elevated levels of fifteen amino acids, notably glutamic acid at 7.97 g/100 g. Moreover, 24 fatty acids were discovered, with oleic acid being most abundant in drones (55.96% in adult, 44.14% in pupa). Furthermore, potassium and sodium were the dominant minerals in both drone and worker samples; however, adult drone had higher concentrations of potassium, iron, copper, and phosphorus than the other samples. Also, nine phenolic compounds were identified, which exhibited the highest concentration in the adult drone sample (20095.72 μg/100 g). Importantly, the adult drone sample demonstrated significant cytotoxic effects on breast and colorectal cancer cells (MCF7 and HCT116), inhibiting them at rates of 98.1% and 81.5%, respectively. These results emphasize the nutritional potential of honeybees (especially adult drones) as a functional food, rich in amino acids, fatty acids and possessing antioxidant and anticancer properties.

Susceptibility of Different Aesculus Species to the Horse Chestnut Leaf Miner Moth: Chemical Composition and Morphological Features of Leaves

The susceptibility of seven species of chestnuts to the horse chestnut leaf miner was studied in the arboretum of the Main Botanical Garden of the Russian Academy of Sciences (MBG RAS), taking into account their interspecific characteristics. Using pheromone delta traps, the highest number of Cameraria ohridella moths was shown for Aesculus hippocastanum and the lowest for Aesculus chinensis. A number of anatomical parameters of leaves were investigated, such as the thickness of the epidermal cell wall and the thickness of the palisade and spongy parenchyma layers. As a result, it was shown that the most infected chestnut species had a greater thickness of the nutritious parenchyma tissue. No dependence was found between the degree of susceptibility to the horse chestnut leaf miner and such indicators as the content of chlorophyll a + b and carotenoids in the leaves of seven species of chestnuts. Nevertheless, resistance of different species of the genus Aesculus to Cameraria ohridella under increased tannin content in leaves has been shown. Evaluation of phenolic compounds and flavonoids has not established their reliable role as repellents. The high levels of carbohydrates found during the study contributed to increased susceptibility to the horse chestnut leaf miner.

Serum metabolomics signature of maternally inherited diabetes and deafness by gas chromatography-time of flight mass spectrometry.

The aim of this study was to identify a metabolic signature of MIDD as compared to healthy controls and other types of diabetes. We performed a comprehensive serum metabolomic analysis using gas chromatography-time of flight mass spectrometry (GC-TOFMS) in participants diagnosed with MIDD (n = 14), latent autoimmune diabetes in adults (LADA) (n = 14), type 2 diabetes mellitus (n = 14), and healthy controls (n = 14). Each group was matched for gender and age. There were significant metabolic differences among MIDD and other diabetic and control groups. Compared with control, MIDD patients had high levels of carbohydrates (glucose, galactose, mannose, sorbose, and maltose), fatty acids (2-Hydroxybutyric acid, eicosapentaenoic acid, and octadecanoic acid), and other metabolites (alanine, threonic acid, cholesterol, lactic acid, and gluconic acid), but low level of threonine. Compared with LADA, MIDD patients had high levels of threonic acid and some amino acids (alanine, tryptophan, histidine, proline, glutamine, and creatine) but low levels of serine. Compared with type 2 diabetes mellitus, MIDD patients had high levels of citrulline, creatine, 3-Amino-2-piperidone, but low levels of ornithine, fatty acids (arachidonic acid and octadecanoic acid), and intermediates of the tricarboxylic acid cycle (malic acid and succinic acid). Our study identified a specific metabolic profile related to glycolysis and the tricarboxylic acid cycle in MIDD that differs from healthy controls and other types of diabetes. This unique metabolic signature provides new perspectives for understanding the pathophysiology and underlying mechanisms of MIDD.

Study on the Function of Leptin Nutrient Acquisition and Energy Metabolism of Zebrafish (Danio rerio).

Leptin plays an indispensable role in energy homeostasis, and its involvement in metabolic activities has been extensively explored in fish. We generated mutant lines of leptina (-5 bp) and leptinb (+8 bp) in zebrafish using CRISPR/Cas9 technology to explore the metabolic characteristics of lepa and lepb mutant zebrafish in response to high glucose nutritional stress induced by high levels of carbohydrates. The results were as follows: the body weight and food intake of adult zebrafish of the two mutant species were increased; the visceral fat accumulation, whole-body crude lipid, and crude protein contents of lepb-/- were increased; and the visceral fat accumulation and crude lipid in lepa-/- zebrafish were decreased. The blood glucose levels of the two mutant zebrafish were increased, the mRNA expression levels of glycolytic genes pk and gck were decreased in the two mutant zebrafish, and there were differences between lepa-/- and lepb-/- zebrafish. The expressions of glycogen synthesis and decomposition genes were inhibited and promoted, respectively. The expression of adipose synthesis genes in the liver and muscle was stimulated in lepb-/- zebrafish but suppressed in lepa-/- zebrafish. Lipolysis and oxidation genes were also stimulated in lepa-/- zebrafish livers, while the livers of lepb-/- zebrafish were stimulated but muscle was inhibited. In conclusion, the results indicate that lepa plays a major role in glucose metabolism, which is conducive to promoting glucose utilization and lipogenesis, while lepb mainly promotes lipolysis and oxidation, regulates protein generation, and plays a minor role in glucose metabolism.

Effect of Lentilactobacillus buchneri on Chemical and Microbial Compositions of Herba Leonuri (Leonurus japonicus Houtt.)-Contained Alfalfa Silage

Lactic acid bacteria (LAB) inoculants are commonly used in silage production, yet their effects on silage containing antimicrobial components, such as those found in Leonurus japonicus, remain less explored. Herein, the harvested alfalfa were thoroughly mixed with dried Leonurus japonicus Houtt. (LJH) at a ratio of 9:1 on a fresh weight basis and treated without (CK) or with a lactic acid bacterial inoculant (L; Lentilactobacillus buchneri). The mixtures were stored under anaerobic conditions in vacuum-sealed polyethylene bags for 30 days at ambient temperature. The L-treated silage exhibited high levels of water-soluble carbohydrates (4.98% dry matter (DM)) and acid detergent fiber (27.88% DM). Compared to that of treatment CK, treatment with L increased the acetic acid content of the silage, as a result of increased (p &lt; 0.05) bacterial dominance and decreased (p &lt; 0.05) bacterial richness indices (e.g., Pielou’s E, Shannon, and Simpson) in the pre-storage period. However, these changes gradually reduced as the storage length increased. Treatment L reshaped the bacterial community structure of silage, by increasing the prevalence of Lactobacillus and reducing relative abundances of Enterococcus and Weissella. However, the principal coordinate and Bray–Curtis index analyses illustrated that samples from the L-treated silages exhibited similarities to the CK samples post-fermentation. Overall, the effect of LJH on LAB was only observed in the later stages of fermentation, which did not sufficiently change the silage quality. Hence, using LJH in silage is vital for clean livestock production without compromising the function of LAB when mixed with alfalfa silage.

Dietary thiamine enhances thiamine transport, carbohydrate absorption, glycolysis, and antioxidant properties in Macrobrachium nipponense when fed a high-carbohydrate diet

Dietary thiamine enhances thiamine transport, carbohydrate absorption, glycolysis, and antioxidant properties in Macrobrachium nipponense when fed a high-carbohydrate diet