Objective: Work-related fatigue is a common health problem among nurses. The objective of this study is to determine the level of fatigue among nurses working at the Adnan Menderes University Hospital in Turkey. Material and Methods: This cross-sectional study has been carried out in Turkey in 2016. A total of 463 nurses from Adnan Menderes University Hospital in Aydin have agreed and completed the data collection form. They have provided information on sociodemographic characteristics, work place, number of shifts worked, work year and weekly working hours. The visual analogue scale was used to measure levels of energy and fatigue. Linear regression analysis was performed to highlight any significant changes. Results: In total, 85.3% of the nurses who participated were females. 60.3% were single, 53.6% worked in intensive care units and 26.1% worked only in the day shift, 17.9% worked for more than 45h a week. According to univariate analyses, nurses who were over 40 years of age, married, worked in internal medicine and surgery clinics for over 15 years and constantly worked in the day shift for 40 h a week had lower energy scores and higher fatigue scores. According to the univariate analysis, all the personal and working characteristics are related fatigue and energy subscale scores. Linear regression analysis revealed that age was the only variable affecting energy scores. Conclusion: It can be stated that factors affecting fatigue among nurses in our study group are associated with age and gender. Prevention and supporting programs may focus on firstly vulnerable nurses (female and older age group).