High Density Research Articles

Impact of flaxseed gum on the aggregate structure, pasting properties, and rheological behavior of waxy rice starch

This study examines the effects of flaxseed gum (FG) on the aggregate structure, pasting and rheological properties of waxy rice starch (WRS). Results display an increase in the ordered molecular structure (R1047/1024), relative crystallinity (RC), compactness (α), and microphase heterogeneity (ε, density degree of nanoaggregates, from 3.52 to 4.23) for WRS-FG complexes. These suggested FG facilitated the development of more organized molecular and crystalline structures of WRS, accompanied by the formation of ordered nanoaggregates with higher density (i.e., nano-aggregation structure). Also, FG addition resulted in the formation of enhanced gel network structure characterized by thicker layer walls and more uniform pores. These structural transformations contributed to a rise in gelatinization temperature (To, from 56.90 °C to 62.10 °C) and enthalpy (ΔH), as well as alterations in paste viscosities (PV, from 1285.00 mPa·s to 1734.00 mPa·s), and the rigidity of network structure (e.g., decreased loss tangent). These results indicate that FG could effectively regulate the techno-functional properties of WRS by rationally controlling the starch intrinsic structures of starch. And this study may improve the pasting and gelling properties of starch, thus driving the development of high-quality starchy foods and prolonging their shelf life, especially for glutinous rice flour products.

Agglomeration behaviors of different types of complex TiN inclusions in high titanium wear resistant steel

Large-sized titanium-containing inclusion clusters seriously affect the continuous casting and performance of high titanium wear-resistant steel. In the current work, thermodynamic calculations were firstly conducted to predict the formation of various complex TiN inclusions, viz, TiOx-TiN, Al2O3–TiN and LaAlO3–TiN. Based on that, laboratory-scale experiments were designed to prepare samples containing a single type of complex TiN inclusions. The agglomeration characterizes of different complex inclusions were compared by combing in-situ observation based on confocal laser scanning microscopy and Newtonian motion equation. Furthermore, the Kralchevsky-Paunov model was used to study the influences of density and size on the agglomeration behavior of complex TiN inclusions. The result shows that the attractive force between TiOx-TiN complex inclusions increases from 1.93 × 10−18 to 4.34 × 10−17 N, while it was 3.41 × 10−17 to 5.12 × 10−16 N for Al2O3–TiN complex inclusions and 6.42 × 10−18 to 1.14 × 10−16 N for LaAlO3–TiN inclusions. Based on the experimental results and theoretical calculation, the agglomeration tendency of Al2O3–TiN is the strongest due to the considered size and compared to the TiOx-TiN inclusions, the stronger agglomeration tendency of LaAlO3–TiN ones can be attributed to their higher density.

Region-specific and age-related differences in astrocytes in the human brain

Astrocyte heterogeneity and its relation to aging in the normal human brain remain poorly understood. We here analyzed astrocytes in gray and white matter brain tissues obtained from donors ranging in age between the neonatal period to over 100 years. We show that astrocytes are differently distributed with higher density in the white matter. This regional difference in cellular density becomes less prominent with age. Additionally, we confirm the presence of morphologically distinct astrocytes, with gray matter astrocytes being morphologically more complex. Notably, gray matter astrocytes morphologically change with age, while white matter astrocytes remain relatively consistent in morphology. Using regional mass spectrometry-based proteomics, we did, however, identify astrocyte specific proteins with regional differences in abundance, reflecting variation in cellular density or expression level. Importantly, the expression of some astrocyte specific proteins region-dependently decreases with age. Taken together, we provide insights into region- and age-related differences in astrocytes in the human brain.

Densification and shaping of pure Cu-BTC powders using a solid-state chemical transformation

MOFs are a class of porous crystalline materials whose unique properties have led to applicability in several fields ranging from gas adsorption to drug delivery. Despite their high potential, MOFs are usually found as fine powders, a property that can limit their use in industrial applications. Here, a novel approach is proposed to form densified Cu-MOF (Cu-BTC) powders and monoliths using 1,2-ethanedisulfonic acid (EDSA) as a densification agent. A MOF/EDSA mixture was heated to ∼150 °C; the molten EDSA not only promotes the growth of larger MOF crystallites, but also stimulates condensation reactions between the carboxylate-based MOF ligands, further binding the particles together. When this reaction was done in a stainless-steel die under pressure MOF-based monoliths could also be formed. Notably, using this approach, the MOF had a higher density, significantly improving the volumetric CO2 adsorption capacity. We believe this contribution provides the basis for future work wherein the intrinsic MOF particle surfaces can be selectively engineered to improve their properties towards shaping for industrial applications.

Dynamical Properties of Magnetized Low-angular-momentum Accretion Flows around a Kerr Black Hole

An essential factor for determining the characteristics of an accretion flow is its angular momentum. According to the angular momentum of the flow, semi-analytical analysis suggests various types of accretion solutions. It is critical to test these with numerical simulations, using the most advanced framework available (general relativistic magnetohydrodynamics), to understand how the flow changes with different angular momentum. By changing the initial condition of the accretion torus minimally, we can simulate a steady, low-angular-momentum accretion flow around a Kerr black hole. We focus primarily on the lower limits of angular momentum and find that an accretion flow with an intermediate range of angular momentum differs significantly from high- or very-low-angular-momentum flows. The intermediate-angular-momentum accretion flow has the highest density, pressure, and temperature near the black hole, making it easier to observe. We find that the density and pressure have power-law scalings ρ ∝ r n−3/2 and p g ∝ r n−5/2, which only hold for very-low-angular-momentum cases. With the increase in flow angular momentum, it develops a nonaxisymmetric nature. In this case, simple self-similarity does not hold. We also find that the sonic surface moves away from the innermost stable circular orbit as the angular momentum decreases. Finally, we emphasize that an intermediate-angular-momentum flow could provide a possible solution to explaining the complex observation features of the supermassive black hole Sgr A* at our galactic center.

Equally Split PCB Inductor (ESPI) Design for High Energy Density and Low Near-Field Radiation

Equally Split PCB Inductor (ESPI) Design for High Energy Density and Low Near-Field Radiation

High-Throughput Sequencing Technology Assisted Investigation of the Correlation between Intestinal Flora, Serum Biochemistry, Blood Lipids, and Tumour Markers in Patients with Gastric Cancer and Healthy Plateau Residents.

Aim The goal is to use high-throughput sequencing technology to compare and study the structure and variety of intestinal flora in people with gastric cancer and healthy people in the Qinghai-Tibet Plateau. Background Recent research has connected gut flora structure to numerous disorders. Metabolites, endotoxins, and immunomodulatory modulation might cause gastrointestinal or other systemic diseases and affect gastric cancer treatment and prognosis. We used the correlation study to uncover biomarkers associated with good intestinal flora and gastric cancer groups on the plateau to investigate their involvement in gastric cancer development. Objectives To investigate the possible links between intestinal flora and gastric cancer in the Qinghai Plateau region using a variety of clinical phenotypic data and to investigate the flora that may be linked to gastric cancer. Methods The 22 Qinghai Province tertiary hospital gastric cancer patients and 30 healthy people had their fresh faeces collected. To examine intestinal flora diversity and composition, 52 patients underwent 16S rDNA high-throughput gene sequencing of intestinal bacteria. The correlation between clinical phenotypes and the top 15 dominant intestinal flora at the phylum level was analyzed. Results The difference in total protein TP between the healthy group and the gastric cancer group was statistically significant (P<0.001). Globulin was significantly different (P<0.05), TC of total cholesterol was significantly different (P<0.05). High-density lipoprotein showed statistical significance (P<0.05).The difference in low-density lipoprotein was statistically significant (P<0.001). Alpha-fetoprotein was significantly different (P<0.05). CA72-4 carbohydrate antigen (P<0.05). Conclusion There were significant differences in total protein, globulin, total cholesterol, high density lipoprotein, low-density lipoprotein, alpha-fetoprotein and carbohydrate antigen CA72-4 in patients with gastric cancer in the plateau area compared with the healthy group, and the different clinical variables were correlated with intestinal flora at some phylum and genus levels.

Simultaneously enhanced electrical properties and high-power characteristics of (K,Na)NbO3 lead-free piezoceramics by hot-pressing

Piezoelectric materials enabling direct conversion between electrical and mechanical energies are ubiquitously implemented in high-power apparatuses, where both superior piezoelectricity and high-power performance are demanded. However, it remains a challenge to simultaneously achieve the two goals, which are hindered by their antagonistic relationship. Herein, hot-pressing is employed to address this dilemma, as exemplified in lead-free (K,Na)NbO3 (KNN) piezoceramics. In contrast to the samples prepared using normal sintering (NS–KNN), hot-pressed KNN (HP–KNN) polycrystals show higher density, lager piezoelectricity (d33∼129 pC/N), and more active domain wall movement. In particular, the normalized strain d33* of HP-KNN piezoceramics exhibits high thermal stability (variation of d33*<10 %) in a broad temperature range from ambient temperature up to 150 °C. Moreover, the HP-KNN samples show superior high-power characteristics with a maximum vibration speed of 1.1 m/s, which is almost 78 % larger than that of NS-KNN. This study not only demonstrates that the HP-KNN lead-free piezoceramics hold great potential for high-power implements, but also opens a new avenue for integrating antagonistic properties for the enhancement of the collective performance in functional materials.

Stability and biological response of PEGylated gold nanoparticles

Stability and cytotoxicity of PEGylated Au NPs is crucial for biomedical application. In this study, we have focused on thermal stability of PEGylated Au NPs at 4 and 37 °C and after sterilization in autoclave. Gold nanoparticles were prepared by direct sputtering of gold into PEG and PEG-NH2. Transmission electron microscopy revealed that NPs exhibit a spherical shape with average dimensions 3.8 nm for both AuNP_PEG and AuNP_PEG-NH2. The single LSPR band at wavelength of 509 nm also confirmed presence of spherical Au NPs in both cases. Moreover, according to UV–Vis spectra, the Au NPs were overall stable during aging or thermal stressing and even after sterilization in autoclave. Based on gel electrophoresis results, the higher density of functionalizing ligands and the higher stability is assumed on AuNP_PEG-NH2. Changes in concentration of gold did not occur after thermal stress or with aging. pH values have to be adjusted to be suitable for bioapplications – original pH values are either too alkaline (AuNP_PEG-NH2, pH 10) or too acidic (AuNP_PEG, pH 5). Cytotoxicity was tested on human osteoblasts and fibroblasts. Overall, both Au NPs have shown good cytocompatibility either freshly prepared or even after Au NPs′ sterilization in the autoclave. Prepared Au NP dispersions were also examined for their antiviral activity, however no significant effect was observed. We have synthesized highly stable, non-cytotoxic PEGylated Au NPs, which are ready for preclinical testing.

Assessment of the association of myofibroblasts and structural components of the extracellular matrix with histopathological parameters of actinic cheilitis and lower lip squamous cell carcinoma.

To evaluate the presence of myofibroblasts (MFs) in the development of lip carcinogenesis, through the correlation of clinical, histomorphometric and immunohistochemical parameters, in actinic cheilitis (ACs) and lower lip squamous cell carcinomas (LLSCCs). Samples of ACs, LLSCCs, and control group (CG) were prepared by tissue microarray (TMA) for immunohistochemical TGF-β, α-SMA, and Ki-67 and histochemical hematoxylin and eosin, picrosirius red, and verhoeff van gieson reactions. Clinical and microscopic data were associated using the Mann-Whitney, Kruskal-Wallis/Dunn, and Spearman correlation tests (SPSS, p < 0.05). ACs showed higher number of α-SMA+ MFs when compared to CG (p = 0.034), and these cells were associated with the vertical expansion of solar elastosis (SE) itself (p = 0.027). Areas of SE had lower deposits of collagen (p < 0.001), immunostaining for TGF-β (p < 0.001), and higher density of elastic fibers (p < 0.05) when compared to areas without SE. A positive correlation was observed between high-risk epithelial dysplasia (ED) and the proximity of SE to the dysplastic epithelium (p = 0.027). LLSCCs showed a higher number of α-SMA+ MFs about CG (p = 0.034), as well as a reduction in the deposition of total collagen (p = 0.009) in relation to ACs and CG. There was also a negative correlation between the amount of α-SMA+ cells and the accumulation of total collagen (p = 0.041). Collagen and elastic density loss was higher in larger tumors (p = 0.045) with nodal invasion (p = 0.047). Our findings show the possible role of MFs, collagen fibers, and elastosis areas in the lip carcinogenesis process.

The effects of land use changes on site occupancy and breeding success of the barn owl (Tyto alba) from 1993 to 2020

Land use changes, driven by human activities such as agricultural intensification and urban expansion, have drastic effects on biodiversity. As these changes are anticipated to continue, understanding species’ adaptations to their evolving habitats is essential to find solutions for effective conservation efforts and sustainable management. In this study, we investigated how long-term changes in the agricultural landscape influence the breeding performance of a population of barn owls (Tyto alba) residing in nest boxes using data collected from 1993 until 2020. Specifically, we assessed how agricultural intensification, urbanization, and site availability affected barn owls' site occupancy and breeding success. Our results reveal that nest boxes surrounded by more agricultural fields are more likely to be occupied and have higher fledging success. Additionally, nest boxes installed higher on the barn were more likely to be occupied. Owls laid more eggs in nest boxes facing North than South or East. Clutches laid in nest boxes installed at higher altitudes were smaller. Finally, nest boxes with a higher density of surrounding nest boxes were less likely to be occupied and had smaller clutches. These insights show barn owls high reliance on the agricultural landscape surrounding their nesting sites, but also the importance of nest box characteristics to optimize their breeding success.

Comparative morphology of the oropharyngeal denticles in the order Rhinopristiformes and its functional implications (Chondrichthyes: Batoidea).

Guitarfishes and sawfishes are included in the order Rhinopristiformes, which currently encompasses five families: Pristidae, Rhinobatidae, Trygonorrhinidae, Rhinidae and Glaucostegidae. Considering the low number of studies focused on oral structures in Rhinopristiformes and the need to better understand their internal morphology, this study aimed to(1) evaluate and describe the morphological variation of the oropharyngeal denticles of guitarfishes and sawfish; (2) evaluate and describe the ontogenetic and sexual variation of the oropharyngeal denticles of Zapteryx brevirostris; (3) propose characters potentially useful for taxonomic and systematic purposes and (4) discuss the possible functions and advantages of these structures. Tissue samples were taken from the oropharyngeal region of specimens preserved in 70% alcohol and then prepared for visualization in scanning electron microscopy. A new method for sampling the pharynx region is proposed herein. Considerable morphological variation between families and genera was observed. However, no variation between conspecifics was found. Regional variations of denticles when examining a single individual were observed in shape, ornamentation, and orientation. In Zapteryx brevirostris, males had a significantly higher density of denticles in the ventral region than females and lower densities were observed in juveniles. The four characters discussed here are based on the presence of keels, number of cusps, distal end elongation and width/length ratio of the oropharyngeal denticles. Among the possible functions and advantages of these structures are the improvement of food adherence, tissue protection against food abrasion and parasitism, and attenuation of hydrodynamic drag in the oropharyngeal cavity during food ingestion.

Modeling Molecular Hydrogen in Low-metallicity Galaxies

We use a suite of hydrodynamics simulations of the interstellar medium (ISM) within a galactic disk, which includes radiative transfer, a nonequilibrium model of molecular hydrogen, and a realistic model for star formation and feedback, to study the structure of the ISM and H2 abundance as a function of local ISM properties. We show that the star formation rate and structure of the ISM are sensitive to the metallicity of the gas with a progressively smoother density distribution with decreasing metallicity. In addition to the well-known trend of the H I–H 2 transition shifting to higher densities with decreasing metallicity, the maximum achieved molecular fraction in the ISM drops drastically at Z ≲ 0.2 Z ⊙ as the formation time of H2 becomes much longer than a typical lifetime of dense regions of the ISM. We present accurate fitting formulae for both volumetric and projected fH2 measured on different scales as a function of gas metallicity, UV radiation field, and gas density. We show that when the formulae are applied to the patches in the simulated galaxy, the overall molecular gas mass is reproduced to better than a factor of ≲1.5 across the entire range of metallicities and scales. We also show that the presented fit is considerably more accurate than any of the previous fH2 models and fitting formulae in the low-metallicity regime. The fit can thus be used for modeling molecular gas in low-resolution simulations and semi-analytic models of galaxy formation in the dwarf and high-redshift regimes.

Onset of Plate Motion in the Presence of Chemical Heterogeneities in the Mantle and the Effect of Mantle Temperature

AbstractChemical heterogeneities of various origins have been observed in the Earth's mantle. Their assumed higher density acts on the style of mantle convection and therefore, as tectonic plates form the highly viscous upper boundary layer of mantle convection, the onset of surface motion is also affected by chemical heterogeneities. We perform 2D basally heated thermochemical mantle convection models which initially include layers of dense material at different mantle depths. In comparison to purely thermal convection models, the onset of first plate motion is delayed. This delay is even more pronounced, the deeper the location of dense material, the larger its volume and the higher its density. Additionally, we varied the starting temperature of our models. In an initially hot mantle, a strong temperature‐dependent viscosity contrast between the mantle and cold surface leads to a cold, highly viscous plate (stagnant lid). For an initially cold mantle, rising plumes first need to transport basal heat upwards to create a sufficient viscosity contrast. Consequently, the formation and subsequent breaking of the stagnant lid is delayed. Considering a hotter mantle after Earth's magma ocean phase, we find that plate motion can occur within approximately the first 0.5 Gyr of solid‐state convection if no chemical structures are present or for dense material situated at the surface. Deep chemical heterogeneities delay the onset by at least one order of magnitude. Furthermore, the early surface motion will have been more erratic than todays stable plate motion, probably with a few single subduction events.

Three-dimensional chromatin landscapes in somatotroph tumour.

The three-dimensional (3D) genome architecture plays a critical role inregulating gene expression. However, the specific alterations in thisarchitecture within somatotroph tumors and their implications for gene expression remain largely unexplored. We employed Hi-C and RNA-seq analyses to compare the 3D genomic structures of somatotroph tumors with normal pituitary tissue. This comprehensive approachenabled the characterization of A/B compartments, topologically associateddomains (TADs), and chromatin loops, integrating these with gene expression patterns. We observed a decrease in both the frequency of chromosomal interactions andthe size of TADs in tumor tissue compared to normal tissue. Conversely, the number of TADs and chromatin loops was found to be increased in tumors. Integrated analysis of Hi-C and RNA-seq data demonstrated that changes inhigher-order chromat in structure were associated with alterations in gene expression. Specifically, genes in A compartments showed higher density and increased expression relative to those in B compartments. Moreover, the weakand enhanced insulation boundaries were identified, and the associated genes were enriched in the Wnt/β-Catenin signaling pathway. We identified the gainedand lost loops in tumor and integrated these differences with transcriptional changes to examine the functional relevance of the identified loops. Notably, we observed an enhanced insulation boundary and a greater number of loops in the TCF7L2 gene region within tumors, which was accompanied by an upregulation of TCF7L2 expression. Subsequently, TCF7L2 expression was confirmed through qRT-PCR, and upregulated TCF7L2 prompted cell proliferation and growth hormone (GH) secretion in vitro. Our results provide comprehensive 3D chromatin architecture maps of somatotroph tumors and offer a valuable resource for furthering the understanding of the underlying biology and mechanisms of gene expression regulation.

The effect of forest structural complexity on tick-borne pathogens in questing ticks and small mammals

Forest management is increasingly focused on enhancing structural complexity. An increase in structural complexity is assumed to increase the abundance and diversity of fauna, including vertebrates. This faunal assemblage serves, in turn, as host community for a suite of vectors and their pathogens, so that structural complexity may cascade through a network of interactions in forest ecosystems. Here we use a network of 19 forest plots representing a gradient of structural complexity and dominant tree species (oak, beech, poplar) to test two hypotheses on the effect of structural complexity on the prevalence of tick-borne pathogens. Our first hypothesis is that tick densities will increase with increasing structural complexity, assuming that each life stage has higher chances of finding a suitable host. The second hypothesis is that the pathogen prevalence in ticks and small mammals decreases (cf. dilution hypothesis) or increases (cf. amplification hypothesis) with increasing structural complexity. These expectations are tested through a community-level analysis, looking at twelve pathogens: seven genospecies of Borrelia (B. afzelii, B. bavariensis, B. garinii, B. burgdorferi sensu stricto, B. spielmanii, B. valaisiana and B. miyamotoi), Babesia s.s., Babesia microti, Anaplasma phagocytophilum, Rickettsia helvetica and Spiroplasma ixodetes. We found that more structurally complex forests have a higher density of questing nymphs (in June and July, during the peak of nymph activity). We did not find a clear change in pathogen prevalence with increasing structural complexity in ticks, wood mice and bank vole; the effect was different for the different pathogens and between the different dominant tree species. No clear co-occurrence patterns of pathogens were found. The density of infected nymphs and thus the disease risk is higher in more complex forests. A potential solution is to focus on decreasing the human-tick interactions in forests instead of trying to decrease the number of questing ticks.

What drives wild boar density and population growth in Mediterranean environments?

Accurate prediction of fluctuations of wildlife local number of individuals is crucial for effective population management to minimise human-wildlife conflicts. Climate, habitat, food availability, and density dependence are among the main factors influencing mammalian population dynamics. In southern Europe, precipitation and temperature, particularly during summer have been suggested as key factors affecting wild boar (Sus scrofa L.). However, there is uncertainty regarding the role of these factors and the mechanisms driving population fluctuations. This study utilized long-term data of wild boar populations from 14 study sites collected for 23 years in Catalonia, Spain, to analyse the factors that drive population density and growth rate. Generalized Additive Mixed Models (GAMM) explained respectively, 94 % and 65 % of the density and growth rate variability. Spring precipitation in both current and previous year, female weight, and forest cover (particularly above 60 %) were directly associated with higher wild boar densities and population growth rates. The interaction between crop cover and total annual precipitation also played a significant role in determining population density. Higher densities were linked to lower population growth in the following year, likely due to a density-dependent process. These results suggest that the expected decrease in rainfall linked with global warming may limit the availability of natural resources and potentially slow wild boar population growth. Nevertheless, wild boar can exploit alternative anthropogenic food sources, potentially leading to an increase of human-wildlife conflicts. Therefore, incorporating management policies aimed at restricting wild boar access to human food sources is key for controlling their reproductive output. Additionally, landscape management strategies targeted at diminishing refuge and resource availability in regions experiencing high wild boar impact are essential for contributing to sustainable coexistence between wild boars and human populations.

STUDY OF PHYSICAL PROPERTIES MECHANICAL PROPERTIES AND THE DEVELOPMENT OF PARTICLEBOARD FROMDURIAN PEEL USING A COMPRESSION MOLDING PROCESS

This research is the process study of producing particleboard from durian peel using the compression molding process. These studies are the physical and mechanical properties of particleboard from durian peel. The results found that the flexural test and the tensile test of furniture material sheets from durian peels at a ratio of durian pulp to glue water were 1:0.2, 1:0.4, 1:0.6, 1:0.8, 1:1, and 1:1.5, respectively. A specimen with dimensions of width 50 x length 200 x thickness 10 millimeters was tested with a machine. The universal testing machine of six samples in each ratio. It shows that the mechanical strength values increased when the ratio of water mixed with glue increased. The durian pulp has a better adhesion. This results are in a higher density as the ratio increases and at the ratio of 1:1.5, it has the highest strength and an average flexural strength of 5.74 MPa but it has not yet passed the criteria set by TISI 876-2004 with a flexural strength of not less than 14 MPa for plywood sheets with a thickness exceeding 6.0-13.0 millimeters. It has an average tensile strength value perpendicular to the surface of 0.49 MPa, which passes the criteria set by TISI standard, with the tensile strength value perpendicular to the surface not less MPa. than 0.40 for plywood with a thickness of more than 6.0-13.0 millimeters. For the testing of the burning rate of particleboard made from durian peels, it was found that the burning rate decreased when the ratio of water mixed with glue increased due to the increasing density of the workpiece at a ratio of 1:1.5 and it can stop the combustion by itself.

Examining Teachers' Professional Relationships, Friendship, and Trust Networks via Social Network Analysis

In this study, Social Network Analysis method was used to determine the professional relationships, friendships and trust networks that teachers established with their colleagues. The study group of the research consists of 35 teachers working in a public secondary education institution in Aydın province. A three-question structured interview form developed by the researchers was used to determine teachers' social networks. Data analysis was carried out through the UCINET 6.0 program. When the findings regarding the positions of teachers in the professional relationship network, friendship network and trust network were examined, it was determined that the network structure with the highest number of connections was the friendship network (ties = 97), followed by the professional relationship (ties = 95) and the trust network (ties = 66). In all social networks, teachers with mathematics, guidance, literature and biology branches were found to be at the center of the network, acting as a bridge between other actors and providing information and resource flow. According to the findings, it was determined that the network with the highest density (dens. = 0.082), bilateral (dia. = 0.198) and triad (triad = 0.330) reciprocity rate was the friendship network. This study, which examines the professional relationships, friendships and trust networks that teachers establish with their colleagues, is thought to be important in revealing the relationships between teachers and analyzing the current situation.

STUDI KEANEKARAGAMAN MIKROALGA DI SUNGAI MARGASATWA RAGUNAN SEKITAR KANDANG BUAYA

Microalgae are microscopic unicellular organisms that have varying sizes, shapes, and types, have photosynthetic pigments, are photoautotrophic, and are found in aquatic environments. Microalgae have certain limits of tolerance to water chemical physics factors. The presence of microalgae in water can be used as an indicator of the state of the water. This study aims to identify the diversity of microalgae as an indicator of the water quality present in the river around the Ragunan Margasatwa crocodile cage. Sampling was done in the Ragunan Margasatwa Park river near the crocodile cage. Samples of macroalgae are visually identified and include morphology, color, shape, and other characteristics. Based on the observations, it can be concluded that the river ecosystems around the crocodile cages are under low ecosystem pressure. The diversity index generally has a range of values between 1.5 and 4, and lower values reflect increasing ecosystem instability. The lowest microalgae density was found at station 1, which is 120 ind/ml. Individuals with the highest density at station 2, the species Aphanothece sp. with a density value of 293.3 ind/mL.