Chitosan, produced through chitin hydrolysis, has been highly appreciated for medical purposes. For the last decade, its application in textiles and biomaterials has grown signifi cantly. It has been well-implemented in man-made fibre, but achieved properties in natural fi bres have not proven durable. Thus, in this study, two chitosans with diff ering degrees of deacetylation (DDA) were implemented into cellulose material using a mercerisation process. The following methods of analysis were used for physical-chemical characterisation: Fourier-transform infrared spectrometry (FTIR), electrokinetic potential (EKA), scanning electron microscopy (SEM) and thermal gravimetric (TG) analysis. For the purpose of studying durability, characterisation was performed after one washing cycle. Performed analyses confi rmed that both chitosans are well-implemented into cellulosic fabric. Fabric treated with chitosan with a higher degree of deacetylation has more positively charged amino groups and better thermal stability.