High Fat Content Research Articles

Wheat β-glucan reduces obesity and hyperlipidemia in mice with high-fat and high-salt diet by regulating intestinal flora.

Unbalanced diets, characterized by high fat or high salt content, are contributing to the obesity epidemic. Wheat bran, recognized as a promising by-product, has the potential to regulate metabolic disorders (MD) associated with obesity. Beta-glucan (BG) has multiple biological activities, but the effect of BG in wheat bran on MD remains unclear. Therefore, this study aimed to investigate the effects of wheat BG (WBG) on dyslipidemia and gut microbiota dysregulation in high fat (HF) or high fat-high salt (HFHS) fed mice. The results demonstrated that WBG significantly reduced the weight gain of mice fed with HF and HFHS diets (from 9.74g to 2.43g and from 6.74g to 2.48g, respectively). Additionally, WBG led to significant reductions in TG (26.26% in HFG and 33.78% in HFHSG) and TC (34.69% in HFG) levels. The liver and adipocyte damage were also reduced after dietary supplementation with WBG. Moreover, WBG significantly reduced Firmicutes/Bacteroidetes ratio (9.52 at HF, 0.62 at HFG, 17.38 at HFHS and 0.61 at HFHSG). Concurrently, there was a reduction in acetic acid levels observed at rates of 26.11% for HF and 32.18% for HFHS. Additionally, WBG reduced the abundance of Coriobacteriaceae UCG-002, Romboutsia, Faecalibaculum, and Enterorhabdus that positively associated with obesity. These changes in gut microbiota may explain the anti-obesity and anti-hyperlipidemia effects of WBG. In conclusion, our findings suggest that WBG is a promising dietary supplement. Our research can provide new insights into the development of foods rich in dietary fiber.

Vegetarian diet - how does it affect our body?

Introduction and Purpose of Research: Vegetarianism, characterized by the exclusion of meat, poultry, fish, seafood, and their derivatives, has gained popularity as studies continue to highlight its positive impact on health. This study aims to comprehensively evaluate the health and lifestyle impacts of vegetarian diets by examining both the benefits and potential risks. Review Methods: The review is grounded in findings from 40 recent studies sourced through a systematic search of open-access databases, including PubMed and Google Scholar, focusing on literature published between 2000 and 2024. Abbreviated description of the state of knowledge: Vegetarian diets are associated with significant health benefits, including reduced risks of chronic diseases such as heart disease, type 2 diabetes, and obesity, largely due to their high content of fiber, antioxidants, and healthy fats. Additionally, vegetarians tend to have lower BMI and improved cholesterol and blood pressure levels. However, unbalanced vegetarian diets may lead to deficiencies in essential nutrients such as protein, vitamin B12, iron, zinc, and omega-3 fatty acids, which can impact overall health. Conclusion: Properly planned vegetarian diets are healthy, nutritionally correct and can be beneficial in the prevention and treatment of certain diseases. Careful selection of meal ingredients and adequate supplementation are key to maximising the benefits and counteracting the potential risks associated with such diets.

Изучение химического СОСТАВа и свойств икры, молок и отходов от переработки БАЙКАЛЬСКОГО ОМУЛЯ

The purpose of the study is to determine the chemical composition and properties of secondary products of processing of Baikal omul (Coregonus migratorius (Georgi, 1775)). Objectives: study of the chemical composition of caviar, milt, heads and entrails of the Baikal omul; mineral composition of fish raw materials; determination of safety indicators of secondary fish raw materials. The objects of the study were secondary products of processing of Baikal omul. The chemical composition of the research objects was determined according to GOST 7636-85 in the research laboratory of the Department of Standardization, Metrology and Quality Management of the East Siberian State University of Technology and Management (East Siberian State University of Technology and Management), the mineral composition was determined in the ANO Center for Biotic Medicine (Moscow). Statistical analysis of the results was carried out using standard statistical methods using MS Excel 2013. The results of experimental studies showed that the fishing season affects the chemical composition of the insides of the Baikal omul, but does not affect the chemical composition of the heads. Thus, the work shows that the entrails of the summer fish are characterized by a lower fat content compared to the omul of the winter fish. Baikal omul caviar is characterized by a high protein and fat content (24.74 and 10.82 %, respectively). Milt contains less protein (10.10 %), but more ash (5.65 %) and moisture (71.90 %) compared to caviar. The main macroelements in the secondary products of the Baikal omul are phosphorus, calcium and potassium, and the microelements are iron, manganese and copper. It has been established that the entrails and caviar of the Baikal omul contain more calcium compared to milt, but the milt is superior to the entrails and caviar in terms of phosphorus and potassium content. Caviar is characterized by a higher content of magnesium, and the entrails have a higher content of zinc, copper and iron. In terms of the content of toxic elements, the milt, caviar and entrails of the Baikal omul met the requirements established by the TR CU 021/2011.

Nutritional Quality of Plant-Based Fish and Seafood Analogs: A Study of the Italian Market

Among plant-based analogs, fish and seafood analogs (PBFSAs) represent a growing sector. This study analyzed the nutritional quality of PBFSAs in Italy and compared it to their animal-based counterparts. Nutritional declarations, ingredient lists, and claims were collected from PBFSA food labeling. Nutri-Scores of PBSFAs and animal-based counterparts were also determined as nutritional quality indicators. Fifty-one products were collected, with most attributed to tuna, salmon, and cod categories (n = 18, 12, and 14, respectively). Results showed large heterogeneity in nutritional quality, with cod products having higher energy (217 (201–257) kcal/100 g), protein (10.5 (7.9–13.0) g/100 g), and carbohydrate (19.4 (14.2–26.0) g/100 g) levels, while tuna and salmon products had a higher fat content (15.0 (10.0–19.7) and 13.5 (5.0–17.0) g/100 g, respectively). Products with fiber or fat nutrition claims did not necessarily indicate higher fiber or lower fat content, while products with a protein claim had a higher protein content. Most animal-based counterparts, except cod and sturgeon caviar, received an “A” Nutri-Score, and often scored better than the PBSFA due to lower salt content. In conclusion, PBFSAs on the market should not be considered animal product analogs regarding nutritional quality, but drawing definitive conclusions is challenging due to the limited number and high variability of the products. However, these findings provide insights that may improve PBFSA nutritional quality, such as decreasing salt and sugar content, for people trying to incorporate such foods into their diet.

Polymorphism of microsatellite loci of yaroslavl cattle in herds of various breeding values

The paper presents the results of studying the polymorphism of microsattellite loci of Yaroslavl cattle in breeding herds of the Yaroslavl region. 115 alleles with different frequency of occurrence were identified for 15 microsatellite DNA loci. From 5 to 11 alleles were identified in each of the studied loci. The average number of alleles per locus was 6.41, the number of effective alleles was 3.58. The highest incidence (0.75) in the entire population was found in locus SPS115 with allele 248, its distribution ranged from 0.72 to 0.83 across farms. For the SPS115 locus, the rare alleles are 252, 254 and 256. 6 alleles were identified at the BM1818 locus. Allele 266 has the highest frequency of occurrence in all the studied herds, and alleles 262, 264 and 270 are present in sufficient numbers. The allele 182 has the highest frequency of occurrence in herds at the BM1824 locus (from 0.46 to 0.64), with an average of 0.51 in the sample. Rare alleles have been identified, which are present only in individual herds with a frequency of occurrence from 0.01 to 0.02. The average number of observed heterozygosity was 0.71, and the expected one was 0.60% less than the observed one. The study found that the first hard the heifers had the highest yield (6871 kg, p < 0.001), rare alleles were identified in this herd – 179 at the CSSM66 locus and 268 at the BM1818 locus. The largest number of alleles, which are not present in other herds, was revealed in the breeding herd III. The higher fat content was obtained from the first heifers of this herd – 4.36% and the higher protein content – 3.45%.The fixation index in all the studied herds was negative (-0.019), which indicates an excess of heterozygotes. The obtained data will allow controlling the level of homozygosity in herds, and taking into account the allelic polymorphism of the breeding stock when fixing bulls of producers to obtain breeding offspring and in custom mating.

Effects of isolation methods on physicochemical properties of defatted starch from the acorn (Quercus brantii).

This study explores the innovative combined effects of alkaline isolation with ultrasound pretreatment on the physicochemical properties of acorn (Quercus brantii) starch. The optimal pH for maximizing the yield of alkaline-isolated acorn starch (AAS) was determined, followed by comparison with alkaline-isolated defatted acorn starch (ADAS), ultrasound-pretreated acorn starch (UAS), and ultrasound-pretreated defatted acorn starch (UDAS). The results demonstrated substantial improvements in yield and purity, with the highest yield (68.97 ± 0.16 %) achieved at pH 9. ADAS showed high purity, with protein and fat contents of 1.82 ± 0.07 % and 0.025 ± 0.02 %, respectively. UDAS exhibited superior swelling power, solubility, and turbidity, indicating enhanced functional properties. Scanning Electron Microscopy (SEM) revealed variations in granule sizes across treatments, from 12.42 μm (ADAS) to 10.72 μm (UDAS). X-ray diffraction analysis showed C-type patterns with crystallinity ranging from 31.25 % (ADAS) to 26 % (UAS). Thermal analysis demonstrated UDAS had the highest peak viscosity and lowest thermal parameters. Texture analysis indicated that AAS formed a softer gel, while ADAS displayed greater hardness and gumminess. These findings highlight the effectiveness of combining alkaline isolation with ultrasound pretreatment to improve acorn starch quality for sustainable applications in food and biotechnology.

Circular Economy on a Small Scale: The Sustainable Use of Olive Tree Biomass Residues as Feed for Lactating Cows in the Sorrento Peninsula

To enhance the sustainability of marginal olive and dairy farms in the Sorrento peninsula, two separate crossover trials were conducted on two farms in the area to evaluate olive pruning residue (OlPr) and olive mill leaves (OlLes) as forage sources for lactating cows. Each trial lasted six weeks and consisted of two treatment periods, each including a 15-day adaptation phase followed by a 6-day measurement phase. During the measurement phase, milk production, feed intake, and olive residue consumption were assessed for two homogeneous cow groups: one receiving a ration supplemented with olive by-products and the other receiving a control diet. The olive-supplemented groups exhibited higher dry matter intake and roughage consumption (hay + olive residue) compared to the control groups. The intake of OlLes was about 30% higher than that of OlPr. Compared to the respective control, milk from OlLe-fed cows a had higher fat content and a higher fat-to-protein ratio, a more favorable fatty acid composition in terms of higher monounsaturated and polyunsaturated fatty acids and conjugated linoleic acid contents, a reduced atherogenic index, and a saturated-to-unsaturated ratio. Likely due to the lower level of olive by-product ingestion, only marginal differences were observed in milk fatty acid composition of cows fed OlPr compared to the control. We conclude that the use of OlLes in dairy cow diets may represent a promising strategy for improving milk quality, promoting a more circular agricultural system, reducing reliance on external feed inputs, and mitigating the environmental impact of both olive and milk production.

Comparative characteristics of different grilled beef cuts and application of torch ginger (Etlingera elatior) flower in seasoning

Grilled beef processed with high temperature has the potential to form toxic compounds that are mutagenic and carcinogenic. This can be prevented by using beef cuts with low-fat content and antioxidant-rich spices, such as torch ginger (Etlingera elatior) flower. This study is aimed to analyze the physicochemical characteristics and antioxidant activity of grilled beef using different beef cuts and application of torch ginger flower in seasoning. Tenderloin and brisket cuts were chosen to represent the low fat and high fat content of the meat. The beef was seasoned with torch ginger flower as one of ingredients for marinade and for dipping sauce, and cooked using grilling method. This study was designed using randomized block design (CRD) with 2 × 6 factorial pattern, consisting of two beef cuts and six seasoning methods with three blocks of the samples manufacturing periods. The differences in beef cuts used to cook grilled beef were related to pH value, water, protein, fat, and malondialdehyde (MDA) content. The use of tenderloin cuts featured lower MDA levels (p ≤ 0,05) than brisket cuts. Tenderloin cuts MDA content was 3,76 mg/kg while brisket cuts had MDA content of 1,67 mg/kg. The application of torch ginger flower in seasoning can increase water content and antioxidant activity while also reduce MDA levels in grilled beef. Soaking of beef in seasoning marinade without torch ginger flower and dipping sauce with the addition of torch ginger flower has resulted to the highest (p ≤ 0,05) antioxidant capacity (135,19 mg EVC g-1), which is more effective compared to other seasoning modes.

Effects of Diets Containing Extruded Linseed and Padina pavonica Algae on Meat Rabbit: Carcass Performance and Meat Quality.

This study investigated the effects of dietary supplementation with extruded linseed (ELS) and Padina pavonica algae extract (PP) on rabbit carcass and meat quality. Ninety-six rabbit carcasses from two production cycles were analyzed. In the first cycle (C1), rabbits were fed a control diet (1CNT), the same diet supplemented with 5% ELS (1ELS5%), and supplemented with 3.5% ELS and 0.2% PP (1LPP3.5%). In the second cycle (C2), the diets varied in composition and supplementation levels: a different control diet (2CNT), the same diet with 5% ELS (2ELS5%), and with 5% ELS and 0.2% PP (2LPP5%). Meat analyses were performed on Longissimus thoracis et lumborum (LTL) muscle for physical properties and on thigh meat (THM) for proximate composition, vitamin E, coenzyme-Q10, cholesterol, fatty acid profile, and mineral content. No significant differences in LTL physical quality were observed in C1, although LTL was brighter in C2 (p < 0.001). THM in C2 had higher fat content (p < 0.001). Dietary supplementation with ELS and PP extract significantly increased polyunsaturated fatty acids (n-3 PUFAs) and improved the n-6/n-3 ratio (p < 0.001) in rabbit meat, demonstrating their positive impact on meat quality.

Untargeted Metabolomics Reveals Key Differences Between Yak, Buffalo, and Cow Colostrum Based on UHPLC-ESI-MS/MS.

Colostrum, abundant in immunoglobulins and growth factors, plays a vital role in supporting immunity. Both yak and buffalo milk are characterized by their high protein and fat content. However, the metabolomic profiles of yak colostrum (YC), buffalo colostrum (BC), and bovine colostrum (CC) remain largely unexplored. The objective of this study is to identify unique metabolites that may impact the nutritional value of colostrum. This study employed ultra-high performance liquid chromatography-electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) for untargeted metabolomics analysis of YC, BC, and CC. The analysis revealed 97, 70, and 75 differentially expressed metabolites in the YC-CC, BC-CC, and YC-BC comparisons, respectively. In comparison to CC, both YC and BC shared common features, including reduced choline levels and elevated O-acetylcarnitine. Moreover, metabolites such as 2-hydroxy-6-pentadecylbenzoic acid, DL-glycerol-1-phosphate, thiamine, L-carnitine, methyl β-D-galactoside, and uridine diphosphate (UDP) were identified as potential biomarkers for YC, while 21-deoxycortisol, D-synephrine, uridine, mannitol-1-phosphate, nonadecanoic acid, and perillic acid were specific to BC. YC has greater advantages in energy supply, antioxidant activity, immune regulation, and cell homeostasis, and BC holds unique significance in physical development and energy balance regulation. These findings provide valuable insights, enabling the selection of unique bioactive metabolites to develop targeted functional foods from colostrum, catering to diverse nutritional needs.

Klebsiella oxytoca facilitates microbiome recovery via antibiotic degradation and restores colonization resistance in a diet-dependent manner

Competition among bacteria for carbohydrates is pivotal for colonization resistance (CR). However, the impact of Western-style diets on CR remains unclear. Here we show how the competition between Klebsiella oxytoca and Klebsiella pneumoniae is modulated by consuming one of three Western-style diets characterized by high-starch, high-sucrose, or high-fat/high-sucrose content. In vivo competition experiments in ampicillin-treated mice reveal that K. oxytoca promotes K. pneumoniae decolonization on all dietary backgrounds. However, mice on the high-fat/high-sucrose diet show reduced pathogen clearance. Microbiome analysis reveals that the combination of Western-style diets and ampicillin treatment synergize in microbiome impairment, particularly noticeable in the presence of high dietary fat content. The diet-independent degradation of ampicillin in the gut lumen by K. oxytoca beta-lactamases facilitates rapid commensal outgrowth, which is required for subsequent pathogen clearance. Our findings provide insights into how diet modulates functional microbiome recovery and K. oxytoca-mediated pathogen elimination from the gut.

Valorization of Oil Cakes in Two-Pot Lactone Biosynthesis Process.

Oil cakes are biomass wastes created by pressing oil from oilseeds. Their chemical composition (including high fat or protein content, a favorable fatty acid profile, and a high proportion of unsaturated acids) makes them valuable raw materials not only in animal feeding but are increasingly gaining popularity in biotechnological processes. This article examines the possibility of valorizing oil cakes using the lipid fraction extracted from them or their raw form in a two-pot biosynthesis process of GDDL-a cyclic ester with a creamy-peach aroma. This study tested five types of oil cakes (hemp seeds, rapeseed, safflower, camelina, and flax), analyzing their physicochemical composition and the fatty acid profile of their lipid fraction. Due to the high content of oleic acid (over 62% lipid fraction) and the wide availability, rapeseed cake was used in the biotransformation process. The synthesis of GDDL involved a three-step process: hydrolysis of triacylglycerols, hydration of oleic acid (via lactic acid bacteria in anaerobic conditions), and β-oxidation (via Yarrowia yeast, aerobic process). The analysis showed that it is possible to produce because of the two-pot biotransformation of approximately 1.7 g of GDDL/dm3. These results highlight the process's potential and justify the feasibility of waste valorization. The proposed biotransformation requires optimization and is a good example of the application of the circular economy in food processing and waste management.

Recent Advances in the Mechanisms of Quality Degradation and Control Technologies for Peanut Butter: A Literature Review.

As the quality of life continues to improve globally, there is an increasing demand for nutritious and high-quality food products. Peanut butter, a widely consumed and nutritionally valuable product, must meet stringent quality standards and exhibit excellent stability to satisfy consumer expectations and maintain its competitive position in the market. However, its high fat content, particularly unsaturated fatty acids, makes it highly susceptible to quality deterioration during storage. Key issues such as fat separation, lipid oxidation, and rancidity can significantly compromise its texture, flavor, and aroma, while also reducing its shelf life. Understanding the underlying mechanisms that drive these processes is essential for developing effective preservation strategies. This understanding not only aids food scientists and industry professionals in improving product quality but also enables health-conscious consumers to make informed decisions regarding the selection and storage of peanut butter. Recent research has focused on elucidating the mechanisms responsible for the quality deterioration of peanut butter, with particular attention to the intermolecular interactions among its key components. Current regulatory techniques aimed at improving peanut butter quality encompass raw material selection, advancements in processing technologies, and the incorporation of food additives. Among these innovations, plant protein nanoparticles have garnered significant attention as a promising class of green emulsifiers. These nanoparticles have demonstrated potential for stabilizing peanut butter emulsions, thereby mitigating fat separation and oxidation while aligning with the growing demand for environmentally friendly food production. Despite these advances, challenges remain in optimizing the stability and emulsifying efficiency of plant protein nanoparticles to ensure the long-term quality and stability of peanut butter. Future research should focus on improving the structural properties and functional performance of these nanoparticles to enhance their practical application as emulsifiers. Such efforts could provide valuable theoretical and practical insights into the development of stable, high-quality peanut butter, ultimately advancing the field of food science and technology.

In vitro effect of omentin-1 on level of other adipokines in granulosa cells from ovaries of Large White and Meishan pigs.

Omentin-1, also known as intelectin-1 (ITLN1), is an adipokine produced by visceral adipose tissue and effect on improve insulin sensitivity, regulate energy metabolism and reproduction. Our previously data has shown that ITLN1 expression in porcine ovarian follicles is influenced by both the level of adiposity and the phase of the estrous cycle. Additionally, recent studies have indicated variations in the serum levels of adipokines in pigs with higher fat content, suggest their common interaction. It was therefore hypothesized that ITLN1 regulates the levels of other adipokines and their receptors in the ovary of pigs with different fat content. The aim of this study was to investigate the in vitro effect of ITLN1 (10-100 ng/mL) on the levels of other adipokines, namely adiponectin, apelin, leptin, chemerin, resistin, visfatin, and vaspin in the culture medium, assessed by ELISA. In addition, the effect of ITLN1 on the mRNA expression of the above-mentioned adipokines with their receptors was investigated using RT-qPCR in granulosa cells (Gc) from normal weight Large White (LW) and fat Meishan (MS) pigs on days 10-12 of the estrous cycle. The results showed that ITLN1 reduced apelin levels, increased leptin, and had no effect on vaspin in the culture medium. ITLN1 was found to have a modulating effect on the mRNA expression of the other adipokines with their receptors in porcine Gc. It can, therefore, be proposed that ITLN1 represents a previously unidentified auto- and paracrine regulator of the functions of ovarian microenvironmental compartments in different types of pig breeds.

Fat, protein content in Hermetia Illucens due to feeding papaya, moringa leaves, and snails

Abstract Indonesia is a tropical country rich in biological resources. However, the main problem currently facing Indonesia is the accumulation of waste, especially organic waste, which accounts for 40.7% of the waste generated by Indonesian society. This research provides innovation in organic waste processing by developing and utilizing maggots by feeding papaya leaves, moringa leaves and snails in reducing organic waste. The research approach used was a quantitative descriptive approach. The study was designed with three different treatments, as follows: Treatment A: Moringa leaves &amp; rice bran; Treatment B: papaya leaves &amp; rice bran; Treatment C: snail &amp; rice bran. Examination of protein content was carried out in three stages, namely deconstruction, distillation, and titration, which were then calculated using the basic method of calculating nitrogen content which was converted into protein content associated with the Kjeldahl method. Meanwhile, the examination of fat content was carried out with a general calculation to determine the fat content in a sample, namely by comparing the weight of the initial sample with the weight of fat that was successfully extracted. The results showed that the highest protein content was found in maggots fed with snail food (40.77%) and the highest fat content was found in maggots fed with Moringa leaves (22.03%). This research can increase the economic value of maggot as animal feed, farmers can choose the right type of feed to produce maggot with the required protein or fat content, and can encourage the development of the maggot industry in Indonesia, both for waste management and as an animal feed commodity.

Influence of selenium additives on butter quality and storage stability

The study's results showed that using Selenium preparations in butter production inhibited oxidative processes occurring during butter storage. In such a product, the oxidative changes of the butter were slowed down, resulting in the product's quality parameters being preserved longer. Before storage, the butter was evaluated in the 45 to 43 points range. After 15 days of storage, the taste and odour scores of the oil ranged from 44 to 40 points. The highest score was assigned to samples with high fat content stored at minus 5°C. The 62.5 per cent fat samples stored at 15°C had the lowest score for this storage period. When stored for more than 30 days, there was a further decrease in the taste and odour score of the oil. It scored between 41 and 37 points during this storage period. In addition, only sweet butter stored at minus temperature remained in the high grade. At the last storage stage (60 days), the butter scored from 38 to 35 points. Summarising the presented data, it should be noted that using selenium in butter production inhibits the development of oxidative processes and slows the deterioration of organoleptic characteristics during product storage. The rate of these processes depends on the storage temperature and the amount of oil in the butter. Due to the use of Selenium at the temperature of Minus 5 °C the shelf life of butter of the highest grade increases by 1.6-2.0 times, at 5 °C, by 1.8-2.4 times and at 15 °C, by 2.4-3.0 times. The non-infinite fatty acids in the milk fat of the cows we study are less than infinite acids, as indicated by the value of glyceride solid fractions and the amount of iodine. The iodine index in cows of the Alatau breed was 28.62, while for those in the third lactation and beyond, it was 28.45. The iodine index values for Holstein cows were 28.86 and 28.7, respectively.

The effect of nitrogen fertilization and the use of biostimulators on the chemical composition of grains of two varieties of corn (Zea mays L.).

The aim of the research was to determine the impact of the use of biostimulators and different nitrogen doses on the yield quality of two varieties of corn grown for grain. The field experiment was carried out in 2015-2017 on an individual farm located in north-eastern Poland (52°30'N and 22°26'E). The following factors were examined in the experiment: group I-two corn varieties: PR38N86 (280 FAO); P8400 (240 FAO) group II-four doses of nitrogen fertilization: control treatment-without nitrogen application (0 kg·ha-1 N) nitrogen doses-80 kg·ha-1 N, 120 kg·ha-1 N, 160 kg·ha-1 N, group III-four types of biostimulators used: (1) control treatment-without the use of a biostimulator, (2) biostimulator containing sodium ortho-nitrophenol, sodium para-nitrophenol, 5-nitroguaiacol sodium, (3) biostimulator containing potassium para-nitrophenolate, potassium ortho-nitrophenolate, potassium 5-nitrovacollate, (4) biostimulator containing molybdenum, zinc. Different doses of nitrogen used in the experiment contributed to an increase in the total protein content in the grain of the tested corn varieties compared to the control treatment. The highest significant increase in protein content by an average of 18.96 g∙kg-1 was obtained after the application of 160 kg N∙ha-1, and after the application of 120 kg N∙ha-1 the value of this feature increased on average by 17.18 kg N∙ha-1. Nitrogen fertilization reduced the starch content and increased the fat content in the tested corn varieties. The lowest starch content was found after the application of 120 kg N∙ha-1 and 160 kg N∙ha-1, while the highest fat content was found in grain tested with 120 kg N∙ha-1. The highest protein content was demonstrated after the use of a biostimulator containing sodium ortho-nitrophenol, sodium para-nitrophenol, and sodium 5-nitroguaiacol. The biostimulators used increased the starch and fat content in the grain of the tested varieties, and the differences between them were statistically insignificant. After using a biostimulator containing sodium ortho-nitrophenol, sodium para-nitrophenol, sodium 5-nitroguaiacol, the protein content increased by an average of 6.0%. The biostimulators used increased the starch and fat content in the grain of the tested varieties, and the differences between them were statistically insignificant. The medium-early variety with FAO 240 was characterized by a higher content of total protein (on average by 7.95 g∙kg-1) and crude fat (on average by 1.5 g∙kg-1) compared to the medium-late variety. All tested varieties were distinguished by the highest concentration of total protein and crude fat in the second, optimal year of the study. The lowest protein content was found in the last year of the study with the highest annual rainfall and the lowest average air temperature, and crude fat content was found in a relatively dry growing season. The opposite tendency was obtained in the case of starch content in the grain of the tested varieties.

Comparison of methods of DNA extraction from &lt;i&gt;Hermetia illucens&lt;/i&gt; larvae

The black soldier fly (Hermetia illucens) is a promising and promising source of animal feed due to its high protein and fat content. For this reason, in 2023, by decision of the Government of the Russian Federation, it was included in the list of agricultural products. Currently, the active use of molecular genetic analysis methods for agricultural purposes continues, including for the study of feed and feed additives. However, today there is too little data in the domestic literature on their use against the black soldier fly. Thus, there is almost no information about DNA extraction methods – the very first stage of any genetic analysis. Thus, the purpose of this study is to compare the effectiveness of existing DNA extraction methods and adapt them to work with Hermetia illucens larvae. In this study, several DNA extraction methods were tested, based on different lysing (SDS, guanidine thiocyanate, CTAB) and chelating (EDTA) agents, as well as lysis durations (1, 2, 3 hours), in comparison with a commercial kit. As a result, it was found that the highest DNA concentration (750 ng/μl) is achieved using the CTAB method, however, when using this protocol, additional purification is necessary. The combined action of SDS and high concentrations of EDTA results in a lower DNA yield (50ng/µl), but does not require additional purification. For the first time, a method based on guanidine thiocyanate was used, which turned out to be quite relevant for this object of study. All of the above methods resulted in comparable or higher DNA yield compared to the commercial GMO-SORB-B kit. Increasing the lysis time to 3 hours when using methods based on guanidine thiocyanate and CTAB leads to increased DNA concentration.

Perkebunan Kelapa Sawit Indonesia yang Berkelanjutan

Palm oil, which carries relatively high saturated fat content and is resistant to oxidation, becomes raw material for various industries, including the food industry, cosmetics and others. Therefore, palm oil is one of the most important oils. Indonesia is the largest palm oil producer in the world. From macroeconomic perspective, palm oil has a very strategic role. Besides being a source of foreign exchange, palm oil is also able to stimulate the people-centred economy and open up large employment opportunities. As technology and the globalization era develop, humans are starting to realize the importance of preserving the nature and environment for the benefit of future generations. Apart from that, global warming, which affects the climate change, is considered increasingly dangerous for humans lives and biodiversity on earth. Deforestation is one of the factors that contributes to the global warming. The expansion of palm oil plantations is linked to deforestation. Thus, the issue of sustainability is started to be raised as a relevant topic. The issue of sustainability concerns three elements, namely, profit, people and planet. Not only the economic benefits, but the preservation of nature and forests, as well as the fulfilment of human rights of the palm oil plantation workers must also be considered. The global challenge, such as the rejection of Indonesian palm oil commodities which are considered unsustainable, is also another driving factor to ensure the implementation of a sustainable system in the palm oil plantation business in Indonesia. Ultimately, preserving Indonesia’s nature and forests, as well as fulfilling the human rights of its workforce, are the interests of Indonesia.

Camel milk extracellular vesicles/exosomes: a fascinating frontier in isolation and therapeutic potential.

Camel milk has a unique composition that sets it apart from other types of animal milk, which has captured the interest of medical and scientific communities. Extracellular vesicles (EVs) mainly contain exosomes (Exos, 30-200 nm) and microvesicles (MVs, 200-1000 nm). Camel milk EVs, particularly Exos, which we named EVs/Exos, have arisen as a fascinating area of scientific inquiry, holding enormous potential for the future of biomedicine due to their anticancer, antibacterial, antidiabetic nephropathy, and immunostimulatory impacts. Camel milk EVs/Exos affect the antioxidant status and oxidative stress differently depending on the target cells. They boosted ROS in cancer cells but improved the antioxidant state in healthy cells. Camel milk EVs/Exos have distinct exosomal lactoferrin and kappa casein mRNAs, which could be responsible for their anticancer and immunomodulatory effects. Due to the high fat content of milk, there is a lack of established protocols for the precise isolation of EVs/Exos from milk, despite the increasing interest in this area of study. This review highlighted the techniques employed for milk EV/Exo isolation and characterization, acknowledging the challenges faced by researchers and the latest advancements in overcoming these hurdles. This review also detailed the potential of camel milk EVs/Exos in therapeutic applications. This comprehensive analysis positions camel milk EVs/Exos at the forefront of scientific inquiry, paving the way for groundbreaking discoveries in the years to come.