Highest Flavonoid Content Research Articles

Effect Of Drying Method On Theantioxidant, Phytochemical, Antinutrient And Selected Chemical Properties Of Turmeric Powder

Background: Turmeric (Curcumin longa) is one of the known spices that have culinary uses as well as medical and medicinal uses. It is a golden spice derived from the rhizome of curcuma longa plant which belongs to the family of Zingiberaceae. Turmeric is a therapeutic plant that is consumed as a flavouring, preservative and colouring agent. Turmeric has anti-inflammatory, antioxidant and phytochemical properties. The main active ingredient in turmeric is curcumin. Curcumin has free radical scavenging properties. Despite the health benefits of turmeric, few people use it partly because of the cumbersomeness of the preparation, staining and drying out of the tubers during storage. This study produced turmeric powder and evaluated the effect of drying method on the antioxidant, phytochemical, antinutritional and chemical properties of the products in view of ascertaining the best processing method in terms of nutrient retention. Materials and Methods: In this randomized controlled study, three kilograms (3 kg) of turmeric rhizome were cleaned, peeled, sliced thinly and shared into three equal parts. One part was shade dried for 5 days, the second, sun dried for 48 h and the third oven dried at 55±10C for 6 h. The dried samples were milled separately and analyzed for antioxidants (curcumin, beta carotene, vitamins C and E), phytochemicals (flavonoids, total phenol, saponin and alkaloids), antinutrients (phytate, tannin and oxalate) and some chemical properties (moisture, fat, calcium, iron, zinc and iodine). Data obtained were analyzed statistically using one way analysis of variance and means separated using Duncan new multiple range test. Significance was accepted at 5 % probability (p<0.05). Results: The beta carotene, vitamin C, E and curcumin contents were high ranging from 24.54 to 27.78, 662.24 to 1397.55, 12.54 to 17.88 and 16.39 to 17.89 mg/100g, respectively, suggesting antioxidant potential of turmeric. The high flavonoid content (7.23 to 9.28 %) indicated that turmeric may have inhibitory activity against disease causing organisms and may act as biological response modifier. The low values of alkaloid and saponin obtained maybe an indication of potency of the product. The low values of phytate (0.88 to 1.1 2 %), tannin (2.02 to 3.96 %) and oxalate (0.15 to 0.20 %) are suggestive of high nutrient bioavailability. The samples exhibited low calcium, iron, zinc, and high iodine values (1.01 to 1.63 %, 0.20 to 0.31, 1.27 to 3.11 and 7.27 to 25.86 percent respectively. Significant (p<0.05) variations existed among the values indicating drying techniques influence. Conclusion: The results obtained from the study showed that method of drying has significant effect on the parameters analyzed. Sun drying or shade drying are preferred for turmeric powder processing.

Development of a method for complex processing of thawed fruits of Aronia melanocarpa Elliot

Introduction. The fresh fruits of Aronia melanocarpa Elliot. are characterized by a high content of vitamins and flavonoids (anthocyanins). The fresh fruits of Aronia melanocarpa Elliot. can be used to make juice. Syrup can be obtained on the basis of the pulp of Aronia melanocarpa Elliot. fruits. To obtain preparations of Aronia melanocarpa Elliot., it is possible to use fruits after freezing. The aim of the work was to develop a comprehensive processing of fruits of Aronia melanocarpa Elliot., subjected to freezing. Material and methods. The fruits of Aronia melanocarpa Elliot. were harvested on the territory of the Republic of Mari El in the autumn of 2023 and were frozen. Juice was obtained by direct pressing from thawed fruits. The pulp of Aronia melanocarpa Elliot. fruits was used to produce aronia fruit syrup. The fruits of Aronia melanocarpa Elliot. after defrosting, as well as juice, pulp and syrup, were examined for the amount of anthocyanins in terms of cyanidin-3-O-glucoside. An industrial sample of aronia fruit syrup was used as a comparison sample. Results and discussion. The amount of anthocyanins in terms of cyanidin-3-O-glucoside was determined by direct spectrophotometry at a wavelength of 534 nm. The syrup obtained on the basis of the pulp of Aronia melanocarpa Elliot. fruits exceeds the amount of anthocyanins in the juice of aronia (0,38±0,02%). The content of the amount of anthocyanins in the syrup obtained on the basis of the pulp of aronia fruits (0,54±0,03%) is significantly higher than in the industrial sample of the syrup obtained on the basis of aqueous extraction from fruits (0,020±0,001%). Conclusion. The possibility of complex processing of thawed fruits of Aronia melanocarpa Elliot. is shown, when juice and syrup of fruits can be obtained from one portion of fruits. The syrup and juice of the fruits of aronia can be used as medicines in the medical practice of the Russian Federation.

Antioxidant Activity, Total Phenolics, and Total Flavonoids Content of Bajakah Tampala (Spatholobus littoralis): The Indigenous Herbal Medicine from Kalimantan

Bajakah tampala (Spatholobus littoralis), a medicinal plant traditionally used in Indonesia, particularly on Kalimantan Island, has garnered interest for its potential health benefits. However, scientific evidence remains scarce. This study investigated the antioxidant activity of S. littoralis extract and its total phenolic and flavonoid content. Ethanol extraction and evaporation were used to prepare the extract. The DPPH method assessed antioxidant activity, while Folin–Ciocalteu and AlCl3 complexation methods quantified total phenolics and flavonoids, respectively. The S. littoralis extract exhibited strong antioxidant activity with an IC50 value of 54.19 ± 8.15 µg/mL. Additionally, the extract contained substantial levels of phenolics (0.649 ± 0.026% GAE) and flavonoids (1.084 ± 0.043% QE). These findings suggest a link between the high phenolic and flavonoid content of S. littoralis extract and its observed strong antioxidant activity.

The Effect of Adhatoda Vasica as A Herbal Supplement on The Performance, Nutrient Digestibility, and Blood Profile of Weaned Pigs

A 60-day trial was carried out to determine the impact of Adhatoda vasica as an herbal supplement on the performance, nutrient digestibility and blood profile of weaned pigs. 40 crossbred male pigs (Yorkshire × Landrace) with initial body weight of 10.21 ± 0.03 kg was distributed into 4 treatment consisting of 10 pigs each. On arrival, animals were quarantined for two weeks and fed basal diet which is adequate in all nutrients according to NRC (2012). Pigs in treatment 1 was fed basal diet without Adhatoda vasica leaf powder while those in treatment 2, 3 and 4 were fed same diet supplemented with Adhatoda vasica leaf powder at 200 g, 400 g and 600 g/kg respectively. A completely randomized design technique was adopted and the animals had unrestricted access to clean fresh water and feed. Quantification of phyto-constituents in Adhatoda vasica leaf powder showed that it contained higher concentration of flavonoids (102.1 mg/g) while steroids had the lowest concentration of 30.41 mg/g. Average daily weight gain was higher among pigs fed treatment 3 and 4, intermediate in treatment 2 and lowest in treatment 1. Average daily feed intake, feed conversion ratio and mortality were significantly (P<0.05) influenced by the treatment. Dry matter, ether extract, crude protein and crude fibre digestibility were influenced (P<0.05) by the treatment. Red blood cell, pack cell volume, mean platelet volume, mean corpuscular volume, mean corpuscular haemoglobin, white blood cell, total protein was significantly (P<0.05) different among the treatment except for aspartate amino transferase, alanine amino transferase and alkaline phosphatase (P>0.05). However, all values were within the established range for healthy pigs. In conclusion, Adhatoda vasica leaf powder can be supplemented in the diet of pigs without compromising their growth and health status.

Effect of Ajwa Date (Phoenix dactylifera) Extract on the Histopathology of Pancreatic Islets in Mice with Diabetes Mellitus

Introduction: It is necessary to develop alternative antidiabetic therapies that are safer and more affordable to overcome the high prevalence of diabetes mellitus in Indonesia. Ajwa dates (Phoenix dactylifera) have a high flavonoid content; hence, this study aimed to investigate their effect on streptozotocin-induced diabetes mellitus mice by examining the number of beta cells and the islets of Langerhans. Methods: Twenty-five mice were divided into five groups: a negative control group (K1), a positive control group (K2), and three treatment groups (P1, P2, and P3). The K2, P1, P2, and P3 groups were induced by 100 mg/kg bw of streptozotocin. Additionally, the P1, P2, and P3 groups received oral treatment using ajwa date methanol extract at different doses of 3, 5, and 7 g/kg bw, respectively. The treatment was administered daily for four weeks. The initial analysis included the homogeneity test and the Shapiro-Wilk test. As the data were non-normally distributed, the analysis proceeded with the Kruskal-Wallis test (p<0.05). Results: The comparative analysis revealed significant differences in the number of beta cells among the groups, with a notable decrease observed in the K2 group and an increase in each treatment group. The measurement of the islets of Langerhans exhibited significant differences among the groups, with p=0.001. Conclusion: The administration of ajwa date methanol extract can affect the number of beta cells and the islets of Langerhans in mice with diabetes mellitus. Highlights: This original study examined the antioxidant compounds derived from naturally sourced ajwa date (Phoenix dactylifera) extract. Ajwa date extract has the potential to protect against histological damage, specifically to beta cells and pancreatic islets, in mice induced by streptozotocin.

Study of antibacterial properties of extracts of the herb Speranskia tuberculata (Bunge) Baill

The article deals with the problem of developing antibacterial drugs of plant origin, focusing on the properties of the herb Speranskia Tuberculata (Bunge) Baill, which is still understudied. The aim was to determine the antibacterial activity of preparations of the herb Speranskia Tuberculata (Bunge) Baill, extracted with different solvents. Materials and methodology. Crude extracts were obtained using four solvents: petroleum ether, ethyl acetate, n-butanol, and aqueous solutions. Subsequently, the dried extracts were weighed and dissolved in dimethylsulfoxide to prepare working solutions with 10 to 500 µg/mL concentrations. Colonies of three types of bacteria, Staphylococcus aureus, Escherichia coli, and Propionibacterium Acnes, were used as the object of the study. The degree of antibacterial activity was determined by measuring the antibacterial zone formed after treating bacteria with different concentrations of extracts compared to the control group (treatment with distilled water). In addition, the total content of phenolic compounds and flavonoids was determined by chemical methods. Antioxidant activity, volume of the dry residue of crude extracts, and content of flavonoids and phenolic compounds were compared with the results of liquid chromatography-mass spectrometry on the presence of antibacterial substances in the extracts known from the literature. It was shown that the ethyl acetate extract among the 4 studied extracts had a clearly expressed antibacterial effect. The calculated value of IC50 for this extract varied between 112‒135 μg/mL for different bacteria. Other solvents did not show a pronounced antibacterial effect. The weakest effect was associated with petroleum ether extracts. At the same time, the mass of the dry residue, when extracted with petroleum ether, was the maximum among all solvents; on the contrary, with ethyl acetate, it was the minimum. Regarding the content of phenolic compounds and flavonoids, the results of the studies were opposite; namely, the maximum concentrations were characteristic of the ethyl acetate extract, and the minimum concentrations were characteristic of the petroleum ether extract. According to the literature, twelve compounds found in the extracts have pronounced antibacterial activity. Suppose the presence of an aromatic ring and a carbon-linked hydroxyl group OH- is taken as the main sign of belonging to phenolic compounds. In that case, 8 of 12 invented antibacterial compounds belong to phenols and flavonoids. Experimentally proven high antibacterial activity of preparations of the herb Speranskia Tuberculata (Bunge) Baill based on ethyl acetate extracts. This property is related to the extract's high content of phenolic compounds and flavonoids.

Evaluation of Proximate, Phytochemicals, Antioxidant Capacity, Enzymatic Inhibition, and Anti-Inflammatory Properties of Avocado Seed Meal as Potential Feed Additive

This study examined the proximate composition, phytochemical profile, antioxidant capacity, enzymatic inhibition, and anti-inflammatory properties of avocado seed meal. The proximate analysis revealed that avocado seed meal is rich in protein (17.32%), fat (15.33%), and carbohydrates (48.73%), with moderate levels of moisture (10.26%), ash (2.39%), and crude fiber (5.97%). Phytochemical analysis indicated high contents of phenols (111.56 mg/g), saponins (96.94 mg/g) and flavonoids (66.66 mg/g), but lower levels of alkaloids (14.60 mg/g), tannins (1.17 mg/g), and steroids (0.89 mg/g). The antioxidant properties assessed showed significant DPPH (50.05%) and FRAP (63.04%) free radical scavenging activities, though lower inhibition of lipid peroxidation (34.88%) and vitamin C content (0.03 mg/g). Enzymatic inhibition assays demonstrated alpha-lipase, alpha-glucosidase, and alpha-amylase inhibition rates of 31.46%, 27.56%, and 50.88%, respectively. Anti-inflammatory properties were also notable, with 24.02% albumin denaturation inhibition and 37.99% antiprotease activity. These findings suggest that avocado seed meal holds considerable potential for nutritional and therapeutic applications as natural feed supplement for livestock and humans.

Buddleoside-rich Chrysanthemum indicum L. extract modulates macrophage-mediated inflammation to prevent metabolic syndrome induced by unhealthy diet

BackgroundMetabolic syndrome (MetS) is a precursor to the development of many diseases (atherosclerosis, diabetes, etc.). It is marked by disruptions in glucose and lipid metabolism, along with hypertension. Numerous types of risk factors contribute to the development of the MetS, inflammation and insulin resistance are present throughout the metabolic abnormalities. Chrysanthemum indicum L. is a traditional Chinese plant used for both tea and medicine, known for its high content of total flavonoids, which are important secondary metabolites. Our research led to the extraction of a Buddleoside-Rich Chrysanthemum indicum L. extract (BUDE) which has demonstrated anti-inflammatory properties. Nonetheless, the specific role and mechanism of BUDE in preventing MetS remain unclear.MethodsThe study initially evaluated the role of BUDE in preventing MetS. Subsequently, it investigated the anti-inflammatory properties of BUDE in the liver and pancreas in response to unhealthy diets. It then examined the level of insulin resistance and pancreatic β-cell function induced by inflammation. Additionally, an lipopolysaccharide (LPS)-induced macrophage inflammation model was used to further investigate the ameliorative effects of BUDE in inflammation.ResultsBUDE has hypotensive, hypoglycemic and hypolipidemic effects. It can also resolve the imbalance between macrophage subpopulations, impede the triggering of the NF-κB signaling pathway, reduce the secretion of inflammatory mediators, ameliorate insulin resistance, and safeguard organs such as the liver and pancreas from inflammatory damage. These effects collectively contribute to preventing the development of MetS.DiscussionBUDE has the ability to modulate macrophage-mediated inflammation, leading to improved insulin resistance. Additionally, it delivers antihypertensive, hypoglycemic, and hypolipidemic effects, offering a potential for preventing MetS.

Phenols, flavonoids and anthocyanins in Solanum lasiocarpum from Borneo: In vitro antioxidant activity and phytochemical profiling via LCMS Q-TOF

Phenols, flavonoids, and anthocyanins are compounds that reported play important roles in plant defence mechanism system. Antioxidant activity is mostly derived from these phytochemical groups, which have a wide range of pharmaceutical properties, including antimicrobial, antitumor, anti-inflammatory, antipyretic, anti-allergy, anti-fertility, hypoglycemic, anti-histamine, antioxidant, antiviral, analgesic, anti-ulcerogenic, nephroprotective, antidiabetic, immuno-secretory, cardiovascular, anti-platelet aggregation, antibacterial, anticancer, and hepatoprotective activities. However, these compounds in Solanum lasiocarpum from Borneo areas are remaining unclear. In this experiment, we performed comprehensive evaluation on effect of extraction solvents on phenols, flavonoids, and anthocyanin's contents and further on the scavenging activity (2,2-diphenyl-1-picrylhydrazyl-hydrate, DPPH; 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid), ABTS; and ferric-reducing antioxidant power, FRAP). High phenolic, flavonoid and anthocyanin contents be found in low polar solvent. Antioxidant assay showed acetone was higher activity with IC50 of 6.36 mg/mL compared to methanol (12.1 mg/mL) and hexane (17.8 mg/mL). Furthermore, liquid chromatography mass spectrometry quadrupole-time of flight (LCMS Q-TOF) mass spectrometer detected N-Acryloylglycine, butoctamide hydrogen succinate, eruberin B, emmotin A and N-Cyclohexanecarbonylpentadecylamine that important in natural polymers, serve as sleep disorders treatment agents, anti-fibrotic property, immunomodulatory activity, and act as differentiator between N-palmitoylethanolamine-hydrolyzing acid amidase (NPAA) compared to fatty acid amide hydrolase (FAAH), respectively. The limitation of this research is the assessment of antioxidant properties related to the polarity of the extraction solvent. Polar solvents may exhibit high scavenging activity, but phenolic compounds, flavonoids, and anthocyanins are found in higher concentrations in non-polar solvents. We encourage further investigation into phytochemical profiles due to the abundance from non-polar solvents.

The Development and Pre-Clinical Anti-Inflammatory Efficacy of a New Transdermal Ureasil-Polyether Hybrid Matrix Loaded with Flavonoid-Rich Annona muricata Leaf Extract.

This study aimed to develop a novel ureasil-polyether transdermal hybrid matrix (U-PEO) loaded with Annona muricata concentrated extract (AMCE), which exhibits potent anti-inflammatory activity. The extract was obtained by maceration, a method that allowed for the extraction of a high concentration of flavonoids (39.27 mg/g of extract). In vivo tests demonstrated that 10 mg/kg of AMCE inhibited inflammation for 6 h. The physicochemical characterization of U-PEO with AMCE was conducted via a thermogravimetric analysis (TGA), while its surface was recorded using atomic force microscopy (AFM). The in vitro macroscopic swelling and release tests demonstrated the hydrophilic profile of the material and the percentage of AMCE released. The TGA results demonstrated that the system exhibited physical compatibility due to the thermal stability of U-PEO. Additionally, the AFM analysis revealed a rough and porous surface, with a particular emphasis on the system with AMCE. The release resulted in the liberation of 23.72% of AMCE within 24 h. Finally, the preclinical tests demonstrated that U-PEO with AMCE was also capable of effectively inhibiting inflammation for 6 h, a duration comparable to that of a commercial formulation. The results permit the advancement of the study towards the development of a transdermal system, thereby rendering its application in clinical studies feasible.

A first de novo transcriptome assembly of feijoa (Acca sellowiana [Berg] Burret) reveals key genes involved in flavonoid biosynthesis.

Acca sellowiana [Berg] Burret, a cultivated fruit tree originating from South America, is gaining the attention of the nutraceutical and pharmaceutical industries due to their high content of flavonoids and other phenolic compounds in fruits, leaves, and flowers. Flavonoids are a diverse group of secondary metabolites with antioxidant, anti-inflammatory, and antimicrobial properties. They also play a crucial role in plant immune response. Despite their importance, the lack of research on A. sellowiana genomics and transcriptomics hinders a deeper understanding of the molecular mechanisms behind flavonoid biosynthesis and its regulation. Here, we de novo assembled and benchmarked 11 A. sellowiana transcriptomes from leaves and floral tissues at three developmental stages using high-throughput sequencing. We selected and annotated the best assembly according to commonly used metrics and databases. This reference transcriptome consisted of 221,649 nonredundant transcripts, of which 107,612 were functionally annotated. We then used this reference transcriptome to explore the expression profiling of key secondary metabolite genes. Transcripts from genes involved in the flavonoid and anthocyanin biosynthesis pathways were identified. We also identified 4068 putative transcription factors, with the most abundant families being bHLH, C2H2, NAC, MYB, and MYB-related. Transcript expression profiling revealed distinct patterns of gene expression during flower development. Particularly, we found 71 differentially expressed transcripts representing 14 enzymes of the flavonoid pathway, suggesting major changes in flavonoid accumulation across floral stages. Our findings will contribute to understanding the genetic basis of flavonoids and provide a foundation for further research and exploitation of the economic potential of this species.

Chemical profile, antioxidant and anti-Alzheimer activity of leaves and flowers of Markhamia lutea cultivated in Egypt: in vitro and in silico studies

BackgroundNowadays Alzheimer’s disease and its treatment methods are global concerns. Patients with this disease have poor prognosis and need supportive treatment. The antioxidant activity, anti-acetylcholinesterase (anti-AChE), anti-butyryl cholinesterase (anti-BChE) and Aβ-amyloid-42 inhibition activities of the ethanolic extracts of both leaves and flowers (LEE and FEE) of Markhamia lutea were assessed. The antioxidant activity of LEE and FEE was evaluated using 2,2-diphenyl-1-picryl-hydrazyl-hydrate, oxygen radical absorbance capacity and ferrozine iron metal chelation assays. Additionally, their total flavonoids and total phenolics were determined. The phytochemicals of LEE were analysed using LC–MS/Q-TOF in both positive and negative modes. Also, molecular docking was done for phytochemicals identified in LEE.ResultLEE exhibited higher antioxidant and anti-Alzheimer activities in all techniques due to its high flavonoids content. LEE showed better activity than donepezil in case of anti-butyryl cholinesterase than both donepezil and rivastigmine in case of Aβ-amyloid-42 inhibition. A total of 62 compounds were tentatively identified using Ultra-performance Liquid Chromatography-Electrospray Ionization-Quadrupole Time-of-Flight Mass Spectrometry (UHPLC–ESI–TOF–MS), viz. 35 flavonoids, 11 phenolic acids, 2 terpenoids, 2 phenylpropanoids derivatives, 7 polyphenols, 3 coumarins and 2 organic acids. The molecular docking of some constituents showed that isorhamnetin-O-rutinoside, sissotrin, 3,5,7-trihydroxy-4'-methoxyflavone (diosmetin), rosmarinic acid, kaempferol hexoside, kampferol-7-neohesperoside, acacetin, taxifolin and apigenin-O-hexoside exert a promising activity as anti-Alzheimer drugs.ConclusionThe LEE of Markhamia lutea contains secondary metabolites that is promising to act as natural antioxidants, acetylcholinesterase, butyryl cholinesterase and Aβ-amyloid-42 inhibition inhibitors, which can aid in the treatment of Alzheimer’s.Graphical

From Waste to Wealth: Exploring the Bioactive Potential of Wine By-Products-A Review.

The present paper explores the biological potential of bioactive compounds present in wine industry wastes, highlighting their valorization to promote sustainability and circular economy. Wine by-products, such as grape pomace and vine shoots, contain a high concentration of polyphenols, flavonoids, anthocyanins and other phytochemicals with antioxidant, anti-inflammatory and anticarcinogenic properties. Both conventional extraction methods, such as solid-liquid extraction, and emerging technologies, including enzyme-assisted extraction, ultrasound-assisted extraction, supercritical fluid extraction, microwave-assisted extraction, pressurized liquid extraction, high-hydrostatic-pressure extraction, and deep natural solvent-assisted extraction (NaDES), are discussed. In addition, the preservation of polyphenolic extracts by microencapsulation, a key technique to improve the stability and bioavailability of bioactive compounds, is addressed. The combination of advanced extraction methods and innovative preservation techniques offers a promising perspective for the valorization of bioactive compounds from wine residues, driving sustainability and innovation in the industry.

Antidiarrheal, anti-inflammatory and antioxidant effects of Anethum graveolens L. fruit extract on castor oil-induced diarrhea in rats.

Between food and medicine, nutraceuticals are widely used in human health for the prevention and treatment of various diseases. This study aims to determine the cytoprotective effects of Anethum gravelons fruit extract (AGFAE) on castor oil-induced diarrhea in rats due to its phytochemical and antioxidant properties. Male rats were divided into six groups of six animals each: Control (C), Castor oil (CO), CO + different doses of AGFAE (50, 100, and 200 mg/kg, b.w., p.o.), and the CO + loperamide group (LOP, 10 mg/kg, b.w., p.o.). In vitro, the chemical composition of aqueous Dill fruit extract showed strong antioxidant activity, with a high content of total polyphenols, flavonoids, and tannins. In our invivo studies, pre-treatment with AGFAE reduced malondialdehyde and hydrogen peroxide levels and maintained normal activity of enzymatic and non-enzymatic antioxidants in the gastric and intestinal mucosa. In addition, we found that AGFAE prophylaxis improved the stability of many plasma biochemical parameters altered by castor oil intoxication, such as C-reactive protein concentrations and alkaline phosphatase activities. We suggest that AGFAE phenolic compounds had significant protection against diarrhea involving several mechanisms such as reducing hypersecretion, peristaltic, inflammation, and preserving the endogenous antioxidant levels.

Antimicrobial and therapeutic potentials of the blackberry extracts against Escherichia coli infection in male albino rats.

Blackberries have garnered attention recently due to their high concentration of bioactive components like anthocyanin and their health advantages. Therefore, this study aims to determine the bioactive profile, antioxidant and antibacterial effects of blackberry extracts (BBEs). Then, evaluate the protective effect of BBEs (20%, 30% and 40%) in a rat model of 2 mL of 4-8 × 106 Escherichia coli ATTC 25922 strain colony-forming unit mL-1 oral infection on the seventh day of the experiment. Rats were divided into six groups: G1: control (C-: normal or negative group), G2: (C+: infected or positive group), G3: infected-treated group by 20% BBE, G4: infected-treated group by 30% BBE, G5: infected-treated group by 40% of BBE and G6: infected-treated group by Gentamicin. The results showed that BBE had a high content of total phenolic compounds, flavonoid, anthocyanin contents, and different vitamins (vitamins A, E and C), reaching 450, 186, 58.83 mg 100 g-1, 2.68, 2.14 and 107.46 mg 100g-1 fresh weight, respectively, which showed great antioxidant and antibacterial effects. Therefore, liver enzymes, kidney function and lipid profiles were significantly higher in the infected group than in the control or infected-treated groups. Furthermore, BBE ameliorated inflammation of the intestine and hepatocyte damage compared to the infected control group. These results suggest that consistent intake of BBE might alleviate hepatic inflammation and the gut microbiota in ways that could significantly impact human health. © 2024 Society of Chemical Industry.

Investigation of Chemical Composition, Antioxidant Properties, and Molecular Docking in Different Roasting Stages of Coffee Beans

AbstractThis study investigates the chemical composition and antioxidant properties of coffee beans at different roasting stages, namely green coffee, filter-roasted coffee, and espresso-roasted coffee. Using a Golden Roaster machine, specific roasting profiles were developed to achieve distinct flavor characteristics: an intense flavor and balanced acidity for espresso, and a balanced, complex taste for filter coffee. Results indicate that filter-roasted coffee exhibits the highest radical scavenging activity, as evidenced by its lowest IC50 value for 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition. Green coffee demonstrates superior iron chelation activity, while filter-roasted coffee contains the highest flavonol content and espresso-roasted coffee has the highest flavonoid content. Bacterial sensitivity tests show that both filter-roasted and espresso-roasted coffee are effective against certain strains, including Klebsiella pneumoniae ATCC 13883. Gas chromatography-mass spectrometry (GC–MS) analysis identifies key compounds such as caffeine and 4,4-dimethyl-3-(3-methylbut-3-enylidene)-2-methylenebicyclo [4.1.0] heptane in filter-roasted coffee, and 2-(2-hydroxyphenyl) buta-1,3-diene in espresso-roasted coffee. Molecular docking and in silico molecule’s absorption, distribution, metabolism, excretion, and toxicity (ADME) studies suggest potential pharmaceutical applications for coffee compounds. These findings provide valuable insights into coffee’s complex chemistry and its health-related properties. Additionally, the importance of coffee profiling in bioprocesses is highlighted by the need to carefully analyze the profiling process to optimize the biological effects and health benefits of these compounds. Coffee profiling not only enhances consumer taste experiences but also contributes to a better understanding of coffee’s potential health benefits by effectively identifying biomolecules and nutrients for use in bioprocesses. Graphical Abstract

Influence of hydroxyl group density in propane alcohols-based deep eutectic solvents: Physicochemical properties and potential application as an extraction medium for bioactive compounds

Deep eutectic solvents (DES) composed of citric acid monohydrate (CAM) as hydrogen bond acceptor (HBA) and alcohol as hydrogen bond donor (HBD) were used for separating bioactive compounds from star anise. This study prepared three propane alcohols-based DES with different hydroxyl (OH) group densities in HBD. FTIR analysis verified the presence of hydrogen bond interactions between HBA and HBD, which strengthened with increasing the OH group densities. Increasing the OH group density in DES exhibited lower water content, but higher thermal stability, higher density, higher viscosity as well as enhanced the water absorption rate. All of the propane alcohols-based DES were miscible in high and semi-polar solvents as well as it also displayed frequency independence characteristics. Moreover, propane alcohols-based DES efficiently extracted bioactive compounds from star anise with high total phenolic and flavonoid contents more than traditional solvents. High bioactive phenolic and flavonoid contents in star anise extracts were the major contributors to potent antioxidant activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH, 84.50 %), azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS, 85.73 %), and ferric ion reducing antioxidant power (FRAP, 3.74 mM FeSO4). Therefore, DES was suggested to be a promising extraction medium for valuable bioactive compounds from natural sources that could be utilized as therapeutic agent for oxidative stress.

Implementation of RSM and ANN Optimization Approach for Natural Deep Eutectic Solvents-Based Extraction of Bioactive Compounds from Orange Peel.

The present investigation has focused on developing an eco-friendly method to extract bioactive compounds from orange peel using natural deep eutectic solvents (NADES). NADES-II composed of choline chloride (ChCl) and ethylene glycol (1:2) and 50% water shows the maximal extraction yield with higher antioxidant activity in terms of DPPH and ABTS scavenging activity with a high total phenolic content (TPC) and total flavonoid content (TFC). The microwave-assisted extraction (MAE) process was optimized using response surface methodology (RSM) and an artificial neural network (ANN). ANN showed a higher value of R 2 and lower values of other statistical parameters when compared to RSM. The ideal extraction conditions were optimized as a 13 min rising time, 52 °C temperature, a 21 min holding time, and a 20 mL/g liquid-to-solid ratio. MAE was compared with the conventional heating-stirring extraction (HSE) method using the NADES-II solvent under optimum conditions. The results show that higher extraction yield and antioxidant capacities (DPPH and ABTS), TPC, and TFC can be obtained from orange peel using the MAE process compared to the HSE process. Overall, this study shows an optimiztic approach for the extraction of bioactive compounds from an orange peel using eco-friendly solvents and microwave technology. It also highlights the potential of this approach for valorizing orange peel waste.

Assessment of phytochemicals, antioxidant, anti-hemolytic, anti-inflammatory and anti-cancer potential of flowers, leaves and stem extracts of.

To evaluate phytochemicals and in vitro biological potential of flowers, leaves and stem extracts of Rosa arvensis. Presence of twenty secondary metabolites was confirmed and then phenolic and flavonoid contents were quantified spectrophotometrically. Fourier Transform Infrared spectroscopy was conducted to ascertain functional groups and antioxidant potential was examined using 2,2-diphenyl-1-picrylhydrazyl scavenging activity, total antioxidant capacity and total reducing power assays. Human erythrocytes were used to assess anti-hemolytic activity and five bacterial strains were examined to determine antibacterial potential of plant extracts. Radish seeds were used to perform phytotoxic activity and cytotoxic potential was evaluated via brine shrimps and PC3 cell lines. Highest phenolic contents were detected in the methanolic extract of Rosa arvensis flower (RAFM) [(151.635 ± 0.005) gallic acid equivalent mg/g] and highest flavonoid contents in the chloroform leaf extract (RALC) [(108.228 ± 0.004) quercetin equivalent mg/g]. Fourier-transform infrared spectroscopy analysis showed the presence of wide range of functional groups. The antioxidant assays indicated highest DPPH scavenging activity [IC50 (23.5 ± 0.6) μg/mL] in the methanolic stem extract (RASM), highest total antioxidant capacity [(265.1 ± 0.9) μg/mL] in RAFM and highest reducing potential [(209.9 ± 0.6) μg/mL] in leaf extract (RALM). Highest anti-hemolytic activity [(90.0 ± 0.5) μg/mL] was recorded in RAFM and brine shrimp cytotoxicity potential [(52.3 ± 0.3) μg/mL] in RASM. The antimicrobial activity was detected highest [(21.1 ± 0.5) mm inhibition zones] in RALM against Streptococcus aureus. In the end, anti-inflammatory and anti-cancer activity results depicted less than 50 % inhibition in the methanolic extracts. CONCLUSIONS: Our findings will be helpful in designing pharmaceutical regimens and therefore, more studies can be recommended to isolate and characterize compounds associated with the biological activities of Rosa arvensis.

Pengaruh Variasi Trietanolamin terhadap Evaluasi secara Fisik Sediaan Krim Body Scrub dari Serbuk Belimbing Wuluh (Averrhoa bilimbi Linn)

Body scrub is a type of cosmetic preparation that has seen increased use, particularly with the incorporation of natural ingredients as its base. Belimbing wuluh (Averrhoa bilimbi Linn.) is a traditional medicinal plant known for its high content of saponins, flavonoids, tannins, glycosides, and formic acid, all of which contribute to its significant antioxidant activity. This makes it a promising candidate for development into a cosmetic product for skin care. This study involved the formulation of body scrub preparations using A. bilimbi powder, with variations in triethanolamine (TEA) as an emulsifying and alkalizing agent to optimize the resulting product formula, which was then tested physically. The A. bilimbi fruit was processed into simplicia, which was subsequently ground and formulated into three different body scrub formulas. These three formulas were subjected to organoleptic tests, homogeneity tests, and pH tests to assess the impact of varying TEA concentrations. The results indicate that TEA influences the texture and pH values of the body scrub preparations. However, the distribution of the preparations based on the homogeneity test results showed no significant differences among the three formulas. The study identified Formula II, with a 3% TEA concentration, as the most optimal formula, meeting SNI standards for pH and demonstrating the best organoleptic properties among the three formulas.