High Consumption Rates Research Articles

Tensile strength of friction stir additive manufactured laminated AA 6061/TiC/GS composites

Abstract With the fast progress of industrial manufacturing, friction stir additive manufacturing has fascinated wide-ranging consideration in the industry due to high material consumption rate. Friction stir additive manufacturing (FSAM), a newly developed solid-phase additive manufacturing technique was employed to fabricate AA6061/TiC/GS composite. The process parameters like tool rotational speed, transverse speed, tool tilt angle and type of tools used in friction stir additive manufacturing were analyzed. Taguchi’s L16 orthogonal array and ANOVA method was used to find the optimum process parameters for the tensile strength. Development characteristics of stirred zone, recrystallization and mixing of reinforced particles will significantly improve the mechanical properties of the fabricated composites. Microstructural investigation and fractography was done by using optical microscopy and scanning electron microscopy (SEM). Corrosion, wear behavior and elemental analysis through EDS was also performed for the fabricated material. The maximum tensile strength of 385.74 MPa was attained under optimal parameters of the tool rotational speed 1,200 rpm, transverse speed 55 mm min−1, and tool tilt angle of 1° for scrolled tapered octagonal tool pin. The findings of the linear regression model showed a minor variance between model and experimental values. Prominent results of the experiment were compared by few other researcher’s findings working in similar area.

Dopamine‐modified Highly Dispersed ZIF‐8 to Promote CO2 Absorption

AbstractCO2 capture technology has been widely used to reduce CO2 emissions, resulting in a variety of capture methods and materials. Among them, the more widely used is the chemical absorption method. However, the chemical absorption method has problems such as high energy consumption and slow absorption rate. In order to solve the above problems, we prepared an effective catalyst to improve the CO2 absorption rate. ZIF‐8 and hydrophilic ZIF‐8‐DA were prepared by in situ synthesis under ambient conditions. The catalytic effect of the hydration reaction of ZIF‐8 and ZIF‐8‐DA was tested using CO2 absorption regeneration experiments under aqueous and alkaline conditions, respectively. The results showed that the synthesized catalysts could significantly enhance the CO2 absorption in water and alkaline solution. The addition of 0.1 wt % of ZIF‐8 in alkaline solution increased the average CO2 absorption by 1.80–15.84 %, respectively, while the addition of 0.1 wt % of ZIF‐8‐DA increased the CO2 absorption by 7.01–25.36 %. In addition, both catalysts had excellent reusability and maintained high performance even after five absorption and regeneration cycles. Polymer dispersibility index (PDI) results showed that ZIF‐8‐DA dispersed more uniformly in aqueous solution.

The Role of Agricultural Technology in Attaining Food Security among Small Farmers in Char Area in Mymensingh District

Farmers in the char areas of Bangladesh are frequently affected by food insecurity due to their low engagement in modern agriculture such as less use of modern agricultural technology. This study evaluates the socio-demographic characteristics of the sample farmers to assess their nutritional status and the factors influencing char dwellers' dietary diversity. Survey data were collected from 70 households, and focus group discussions were conducted using a semi-structured questionnaire from Nilokhiya char under the Mymensingh District of Bangladesh. An OLS regression model was used to estimate the influence of socioeconomic characteristics of sample households on the decision on factors influencing dietary diversity. The farming type of the farmers is another concern of this study where results show that 90% of farmers in the char areas rely on rice and vegetable farming, whereas more than 60% of the farmers are also involved in livestock and poultry farming at the same time. The nutritional status of char farmers shows that their diet includes a variety of foods but is deficient insufficient protein and healthy fats, which could have an impact on their overall nutrition. Cereals are a staple in the diet, as indicated by the high consumption rate (97.1%) and the substantial average intake (2.30 kg/day). Every household consumes fruits, though in smaller quantities (0.28 kg/day). Eggs are less frequently consumed (57.1%) despite a higher intake (2.64 kg/day). Dietary diversity has a strong positive correlation with supply (.913) at a 1 % significant level which means that efficient supply chains minimize delays, reduce spoilage, and ensure a continuous flow of food to markets to ensure food security. A positive correlation with total income (.373) leads to a higher total income also supports greater dietary diversity. Credit was significant at a 5% level (.267) indicating that a 1 % increase in credit leads to an increase in dietary diversity by 267%. On the contrary, Experience has a negative relationship with dietary diversity which means that experience does not influence food security. Lack of transport and communication, environmental hazards, and natural disasters are the most significant problems faced by the farmers in the char area.

Evaluating the barriers to adoption of improved biomass cookstoves in Benue state, Nigeria

The prevalent use of inefficient traditional stoves for biomass cooking energy in Nigeria contributes to high rates of biomass consumption, deforestation, and harmful pollutant emissions. This study aimed to assess the constraints hindering the adoption of improved biomass cookstoves (IBCS) in Benue State, Nigeria. Data were randomly collected from 135 households and analyzed using descriptive (frequency, mean and percentage) as well as inferential statistics (binary probit regression). Findings revealed that 88% of households rely on fuelwood for cooking, with approximately 35% still using traditional stoves. However, 46% of households have adopted IBCS and recognized its advantages, including improved cooking speed, fuel economy, time savings in fuelwood collection, cost savings, and reduced smoke emissions. Additionally, various factors such as age, household size, access to credit, location, distance to market, membership of women's associations, fuelwood collection distance, and fuelwood sources were identified as constraints to IBCS adoption. To promote IBCS adoption, we recommend that development planners and extension officers raise awareness among households and provide them credit incentives through government and NGO support. This research significantly contributes to pertinent knowledge by shedding light on the challenges and opportunities associated with IBCS adoption in Nigeria, thereby informing policy and development initiatives in the country.

Assessment of Common Ragweed (Ambrosia Artemisiifolia L.) Seed Predation in Crop Fields and Their Adjacent Semi-Natural Habitats in Hungary

Ambrosia artemisiifolia has turned into a noxious weed species in agricultural fields and landscapes in Europe. Durable control options are still needed to limit the abundance of this species. Weed seed consumption by naturally occurring seed predators is a key ecosystem service in agricultural areas. Seed predation levels of common ragweed were examined in wheat and maize fields and adjacent semi-natural habitats (SNHs). To evaluate the weed seeds’ exposure to invertebrate seed predators, 20 cards each were set on the soil surface inside the crop field and in SNHs with four replications. Twenty seeds of ragweed were attached to sandpaper. Seed removal was assessed every 24 h of exposure for 5 days in June and November 2019, October 2020, and June 2021. The seed consumption level was measured according to the number of removed seeds from the seed cards. High consumption rates of ragweed seeds were found in all sampling rounds in both seasons and habitats. The seed predation rates in 2019 were stronger within crop fields in summer than in autumn with a slight difference between SNHs and inside fields. Our results demonstrate the possibility of seed predation contributing to Integrated Plant Protection (IPM) of common ragweed in rural areas.

‘All the dykes I know drink beer’: Sexuality and gender performance through alcohol consumption among lesbian, bisexual, and queer women in Australia

This article explores the relationship between gender, sexuality and alcohol consumption among lesbian, bisexual, and queer (LBQ) women in Australia. On average, LBQ women consume alcohol at a higher rate than heterosexual women, yet the use of alcohol and its gendered meanings in this population remain under-explored. We conducted semi-structured interviews with 42 cisgender women, 10 transgender women, and 8 non-binary people who identify as lesbian, bisexual, and/or queer. Participants were aged 18–72. Interviews explored participants' experiences with alcohol, gender, and sexuality. Interviews were thematically analysed, informed by a critical alcohol and other drug scholarship lens. While ‘minority stress’ is often cited as a key factor in explaining LBQ women's high rates of alcohol consumption, our findings suggest a more complex relationship between drinking, gender and sexuality. Drinking is gendered for LBQ women, enabling both performance of and resistance to gendered norms. Additionally, alcohol emerged as a tool for navigating gendered vulnerability in public spaces, with some participants shaping their alcohol intake to their environment. Our findings highlight the need for approaches to LBQ women's health that recognise alcohol as a social tool that can facilitate confidence, pleasure, and community connection. Rather than focusing solely on risk and vulnerability, policies and health promotion efforts should consider the diverse ways in which LBQ women use alcohol to navigate their identities and social environments.

Abstract A092: Tobacco use and prevalence of oral premalignant lesions, among Malayali tribes, Yelagiri Hills, Tamil nadu, India

Abstract BACKGROUND: India has the second largest tribal population of the world next to the African countries. About half of the world’s autochthonous people live in India, thus making India home to many tribes which have an interesting and varied history of origins, customs and social practices. The present study was conducted to assess the tobacco use and prevalence of precancerous lesions and oral cancer among Malayali tribes, Yelagiri Hills, Tamil nadu, India. METHODOLOGY The inhabitants of the 14 villages of the Yelagiri hills, who have completed 18years and residing for more than 15years present on the day of examination and who were willing to participate in the study were included. Data was collected from a cross-sectional survey, using a Survey Proforma, clinical examination and a pre-tested questionnaire. An intra-oral examination was carried out by a single examiner using WHO Oral Health Surveys – Basic Methods Proforma (1997). ResultsThe overall prevalence of smokeless and smoking tobacco was 78.8% and 65.2% respectively among 1550 study population. Of those who had the habit of smoking, 26% smoked beedi, 10.9% smoked cigarette, 65% chewed raw tobacco, 18% chewed Hans and 28% had a combination of smoking and smokeless tobacco usage. Around 49.8% of samples were stained positive for precancerous and cancerous lesions of the oral cavity. The highest number of positive samples were from buccal mucosa with 46.2% subjects and 12% for labial mucosa. The reason for practicing these habits were as a measure to combat the cold, relieving stress and body pain after work, and the lack of awareness of the hazards of the materials used. Prevalence of oral mucosal lesions in the study population was due to tobacco usage and alcohol consumption and lack of awareness regarding the deleterious effects of the products used. CONCLUSION Oral cancer is highly prevalent in this Malayali tribes owing to high tobacco consumption rates and habits warranting the cessation center a priority. Also, early detection and screening are vital to attaining better outcomes. Citation Format: Delfin Lovelina Francis. Tobacco use and prevalence of oral premalignant lesions, among Malayali tribes, Yelagiri Hills, Tamil nadu, India [abstract]. In: Proceedings of the 17th AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2024 Sep 21-24; Los Angeles, CA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2024;33(9 Suppl):Abstract nr A092.

Evaluating dietary exposure and risk characterization of aflatoxin B1, citrinin, and ochratoxin A co-occurrence in pigmented rice consumed in Thailand

In recent years, pigmented rice, often referred to as functional rice, has attracted increasing attention due to its perceived health benefits arising from an abundance of phytochemicals. However, there is limited information on the occurrence and risks of mycotoxins due to long-term consumption of pigmented rice. This study assessed the co-occurrence and contamination levels of aflatoxins, citrinin (CIT), and ochratoxin A (OTA) in various pigmented rice samples sourced from Thailand, and also evaluated the potential health risks for consumers. The results indicated varying levels of mycotoxin contamination among the different tested pigmented rice varieties, with aflatoxin B1 (AFB1) being the most prevalent (39.1 %), followed by CIT (17.3 %) and OTA (14.5 %). The co-occurrences of AFB1-CIT and CIT-OTA were the most prevalent combination observed in the pigmented rice samples. Although the AFB1 and CIT levels remained below regulatory limits, some samples had OTA levels exceeding these limits, though by a small percentage. Furthermore, the anthocyanin content in pigmented rice showed a weak positive correlation with the AFB1 level (p < 0.05). The estimated risk of liver cancer incidence associated with the consumption of pigmented rice containing AFB1 was in the range 0.012–0.061 cases/100,000 persons/year, depending on consumption rate and behavior. The risk posed by CIT exposure was considered negligible. However, the exposure assessments suggested a potential risk of OTA exposure, particularly in scenarios with higher consumption rates. Cumulative risk assessment of AFB1-OTA co-occurrence indicated an amplified health risk compared to single mycotoxin exposure. These findings offer valuable evidence, encouraging further research aimed at mitigating the risks associated with mycotoxin contamination in pigmented rice.

Status of Production, Consumption, and End-of-Life Waste Management of Plastic and Plastic Products in Nigeria: Prospects for Circular Plastics Economy

This study is motivated by the need to understand and proffer sustainable circular economy solutions to the persistent challenges associated with plastic waste management in Nigeria. Despite the emerging awareness and increased number of studies conducted on plastic and plastic products in Nigeria, the challenges and opportunities associated with their production, consumption, and post-consumption management are still poorly understood. Besides the large quantity of plastic and plastic products produced locally in Nigeria, a substantial quantity of these products in various forms and polymer types also comes into the country through importation. This results in a high consumption rate and, by extension, a huge volume of plastic waste generated daily exceeding the capacity and ability of the authorities to manage. In this work, we reviewed the available literature to analyze the status of the production, importation, consumption, and post-consumption management of plastic waste in Nigeria. It is estimated that out of 27.3 million tonnes of municipal solid waste currently generated in Nigeria’s urban cities per annum about 11.2 million tonnes is collected, of which about 1.1 million tonnes is plastics. It is further projected that by 2040, about 40.5 million tonnes of municipal solid waste will be generated, and only about 1.6 million tonnes of the plastic component will be collected for disposal. Based on the outcome of the analysis, the current study further suggested how adopting circular economy principles can help mitigate the impact of plastic waste on Nigerian society.

Excitation–contraction coupling reflects the metabolic profile of mantle muscle in young cuttlefish (Sepia officinalis)

AbstractThe mantle muscle of common cuttlefish, Sepia officinalis, is responsible both for high‐magnitude and rapid movements for locomotion, as well as sustained ventilation, which require specific metabolic, electrophysiological, and structural organization. Young cuttlefish have a highly oxidative phenotype and a rapid growth rate. Here, we show high rates of oxygen consumption and protein synthesis in juveniles, and these rates decay exponentially over the first few weeks of growth. This is associated with considerable citrate synthase activity (relative to larger cuttlefish) but a lack of glucose metabolism based on zero uptake of glucose by isolated muscle sheets and minimal activity of hexokinase (similar to larger animals). In contrast to glucose metabolism in the heart, glucose metabolism in these muscle sheets was not stimulated by extracellular taurine. Previous research revealed an unusual ion channel complement in mantle myocytes, the most notable feature of which is the lack of a Na+ current during depolarization. Because this adaptation is not consistent across the coleoid clade, we investigated excitation–contraction coupling. Here, mantle energetics and contractility, including the individual components of the total Ca2+ flux driving contraction, were studied. Results indicate that the majority of Ca2+ current underlying contractile stress development capacity in cuttlefish juveniles is not mediated by dihydropyridine‐sensitive L‐type channels, in contrast to their adult counterparts, and the sarcoplasmic reticulum contributes little to routine contractility. We had previously noted an influence of physiological levels of taurine in limiting cardiac contractility but found no analogous sensitivity in mantle muscle. Finally, transmission electron microscopy of subcellular architecture revealed the presence of sarcoplasmic tubular aggregates, suggesting that oxidative inhibition of sarcoplasmic reticulum function limits its role in this life stage.

Stable isotope analysis reveals fish juveniles as a temporal main resource consumed by invasive pumpkinseed (Lepomis gibbosus)

AbstractThe pumpkinseed Lepomis gibbosus (Linnaeus, 1758) is a small centrarchid fish species from North America that has invaded most European basins, with pronounced expected impacts. Analyses of pumpkinseed diet in its invasive range using gut content analysis (GCA) show macrozoobenthos as the most common dietary item, suggesting a competitive effect on native fish communities. Our study uses a combination of GCA and stable isotope analysis (SIA) to document pumpkinseed diet at two sites in its invaded range and relates pumpkinseed diet preferences with parasitic load. SIA revealed juvenile fish as a major prey item at one of the sites (63 and 50% of the diet of small and the large fish), while GCA showed pumpkinseed preying mostly on macroinvertebrates (chironomids, gastropods, Ephemeroptera, and Zygoptera larvae) at both sites. While infrequent infection by trophically transmitted parasitic nematodes could be related to low consumption of zooplankton, substantial infection by metacercariae of Posthodiplostomum centrarchi Hoffman1958 appears to reflect relatively high consumption rates of its intermediate host, physid snails. Using SIA allowed to demonstrate that pumpkinseed feeding in its non-native range can be based on piscivory, emphasising the need for combining multiple methods when studying the impact of non-native species on food webs.

Designing a Novel Temperature- and Noncanonical Amino Acid-Controlled Biological Logic Gate in Escherichia coli.

Genetic code expansion (GCE) is a powerful strategy that expands the genetic code of an organism for incorporating noncanonical amino acids into proteins using engineered tRNAs and aminoacyl-tRNA synthetases (aaRSs). While GCE has opened up new possibilities for synthetic biology, little is known about the potential side effects of exogenous aaRS/tRNA pairs. In this study, we investigated the impact of exogenous aaRS and amber suppressor tRNA on gene expression in Escherichia coli. We discovered that in DH10β ΔcyaA, transformed with the F1RP/F2P two-hybrid system, the high consumption rate of cellular adenosine triphosphate by exogenous aaRS/tRNA at elevated temperatures induces temperature sensitivity in the expression of genes regulated by the cyclic AMP receptor protein (CRP). We harnessed this temperature sensitivity to create a novel biological AND gate in E. coli, responsive to both p-benzoylphenylalanine (BzF) and low temperature, using a BzF-dependent variant of E. coli chorismate mutase and split subunits of Bordetella pertussis adenylate cyclase. Our study provides new insights into the unexpected effects of exogenous aaRS/tRNA pairs and offers a new approach for constructing a biological logic gate.

Functional response metrics explain and predict high but differing ecological impacts of juvenile and adult lionfish.

Recent accumulation of evidence across taxa indicates that the ecological impacts of invasive alien species are predictable from their functional response (FR; e.g. the maximum feeding rate) and functional response ratio (FRR; the FR attack rate divided by handling time). Here, we experimentally derive these metrics to predict the ecological impacts of both juvenile and adult lionfish (Pterois volitans), one of the world's most damaging invaders, across representative and likely future prey types. Potentially prey-population destabilizing Type II FRs were exhibited by both life stages of lionfish towards four prey species: Artemia salina, Gammarus oceanicus, Palaemonetes varians and Nephrops norvegicus. FR magnitudes revealed ontogenetic shifts in lionfish impacts where juvenile lionfish displayed similar if not higher consumption rates than adult lionfish towards prey, apart from N. norvegicus, where adult consumption rate was considerably higher. Additionally, lionfish FRR values were very substantially higher than mean FRR values across known damaging invasive taxa. Thus, both life stages of lionfish are predicted to contribute to differing but high ecological impacts across prey communities, including commercially important species. With lionfish invasion ranges currently expanding across multiple regions globally, efforts to reduce lionfish numbers and population size structure, with provision of prey refugia through habitat complexity, might curtail their impacts. Nevertheless, the present study indicates that management programmes to support early detection and complete eradication of lionfish individuals when discovered in new regions are advised.

Production of an Ice Cream Base with Added Lacticaseibacillus rhamnosus GG and Aguamiel Syrup: Probiotic Viability and Antihypertensive Capacity

Given the rising interest in functional foods for health benefits, this study aims to evaluate the antihypertensive activity of an ice cream base incorporating Lacticaseibacillus rhamnosus GG and aguamiel syrup. We assessed the probiotic viability and ACE inhibitory activity in ice cream enriched with aguamiel syrup compared to inulin. Several reports have highlighted the importance of consuming symbiotic dairy foods to modulate the intestinal microbiota and multiple pathophysiologies. Ice cream has a high worldwide consumption rate, so it is an alternative to incorporating probiotics and prebiotics. The probiotic was inoculated (109 CFU/mL) into an ice cream base enriched with aguamiel syrup and a control base with added inulin. The carbohydrate profiles in the aguamiel (used to produce the syrup) and the aguamiel syrup were obtained through HPLC. TNBS and SDS-PAGE analysis were used to determine the proteolytic action of the probiotic. Sucrose was the carbohydrate with the highest concentration in fresh aguamiel and aguamiel syrup. The probiotic remained viable for 14 days under refrigerated storage conditions, with the aguamiel syrup base showing superior protein hydrolysis (free amino groups 302.67 ± 2.29 µg/mL) and 65% ACE inhibition. Likewise, the pH remained unchanged throughout the refrigerated days. These results underscore the potential of aguamiel syrup as a prebiotic in functional dairy products.

Oil Palm Plantation Expansion and Population Problems: An Explanatory Factor for Deforestation in Indonesia

Deforestation in Indonesia remains a significant issue due to various factors. This article reveals the impact of demographic variables on deforestation, using oil palm plantation expansion as a case study. The study applies Hunter's model, considering population dynamics and intermediate variables, namely science and technology, institutions, policies, and culture. Through traditional literature review methods, demographic data and field research results from 2010 to 2020 were elaborated, focusing on Sumatra, Kalimantan, and Sulawesi. The findings underline that Indonesian rapid population growth drives high consumption rates. Additionally, the development of oil palm plantations is faced with high demand for CPO, policies that attach too much importance to investment, and agrarian deregulation. All these intermediate variables are visible through the agricultural expansion as a pattern of agricultural development. The massive expansion by private companies resulted in a change in the socio-ecological landscape. In addition, there are dilemmas in the villager's livelihood system, such as shifting traditional livelihood bases, livelihood dualism, the emergence of new vulnerabilities, and land grabs. In order to realize sustainable palm oil development, we suggest governance reforms at the global, national, and local levels.

Enhancing Alcohol-Related Research in Africa: Possibility for a Continental-Wide Alliance.

Alcohol-related harms constitute a major public health concern in Africa, with high rates of alcohol consumption and associated negative health and social consequences. Despite this, there is a lack of comprehensive and coordinated research on alcohol use and related harms across the continent. This paper discusses common factors such as diverse traditions and cultures, prevailing low socioeconomic status, gendered approach, limited policy and weak regulation and compliance to existing alcohol control policies that contribute to alcohol-related problems, and the challenges facing alcohol-related research including limited research, isolated and uncoordinated research, and resource scarcity; it further proposes strategies for advancing a continental-wide alliance to enhance alcohol-related research in Africa. The alliance would bring together researchers, policymakers, and other stakeholders to collaborate on research projects, share data and resources, and develop evidence-based interventions to reduce alcohol-related harms. Furthermore, the paper outlines the potential benefits of such an alliance, including improved data collection and analysis, greater capacity building for researchers, and more effective policy and program development. The paper concludes that a continental-wide alliance on alcohol-related research in Africa has the potential to significantly enhance our understanding of alcohol use and related harms on the continent and to inform effective prevention and intervention strategies.

Comparison study of food, energy and nutrient intake by 24-hour dietary recall method on two consecutive days versus three consecutive days

To compare the differences in estimated food, energy, and nutrient intakes between the consecutive 3 days 24-hour dietary recall(24HR)(referred to as the 3-day method) and consecutive 2 days 24HR(referred to as the 2-day method) to provide a basis for the use of consecutive 2 days 24HR in China nutrition surveillance. Using objective sampling to select participants in northern and southern provinces, dietary data were obtained through consecutive 3 days 24HR, and the average intakes of food, energy and nutrients were calculated for three days from Thursday to Saturday and two days on Friday and Saturday, respectively. The 3-day method was considered as the reference standard method to evaluate the performance of the 2-day method for estimating food, energy and nutrient intakes. Among 778 participants aged 18-60 years in urban and rural areas of two provinces, the errors of the mean and median of 2-day method for estimating the intake of four major food categories less than 6% compared with the 3-day method, and there were significant equivalencies(P&lt;0.025) and no significant differences in four major food categories group(P&gt;0.05). Of the 24 food groups estimated by the 2-day method, 17 had mean errors within 5%, the largest error was in animal offal(13.45%) and the smallest in fruit(0.15%), and there were significant equivalencies(P&lt;0.025) and no significant differences in seven food groups(P&gt;0.05). For energy and nutrients, the mean and median errors of energy were less than 0.5% and there were significant equivalencies(P&lt;0.025) and no significant differences for energy(P&gt;0.05). Among the 25 nutrients, except sodium, iodine and vitamin E, the mean and median errors of the other 22 nutrients were less than 5%, and there were significant equivalencies(P&lt;0.025) and no significant differences in 16 nutrients(P&gt;0.05). There was little difference between the two survey method in assessing the intake of high consumption frequency foods, energy and most nutrients at group level, the 2-day method can be used as an alternative to the 3-day method to collect dietary intake data with high consumption rates in the population.

Nanotechnology approaches for Enhancement in Biohydrogen Production

The rapid surge of renewable energy sources has been influenced by the high rate of energy consumption and the low sustainability of traditional energy sources. Being an excellent energy source, hydrogen does not leave any negative carbon footprint as it only produces water during the combustion process. It is carbon neutral which can be produced from a variety of waste feed stocks or biomass, making it the most efficient and environmentally friendly form of energy amid all biofuels. To meet the future hydrogen demand, biological processes like bacterial fermentation, are considered to be environmentally favourable option. Since biomass is abundant, cheap, and biodegradable it is considered profitable for biohydrogen production. Though photo-biological and dark fermentation methods are regarded as successful in generating biohydrogen, their lower yields pose significant challenges for its commercial production. Studies are being conducted to improve efficiency, and here is where nanomaterials come into play by influencing biological processes at the cellular level. They can act as catalysts speeding up the reactions that create hydrogen and making the process more sustainable. Owing to their distinct properties such as stability, crystalline nature, high ratio of surface to volume, adsorption ability, and increased electroconductivity significantly enhance hydrogen generation. In this paper, the applications of nanomaterials such as metals, metal alloys, metal oxides, nanocomposites, and inorganic nanoparticles to improve biohydrogen production have been studied.

Exploring the Relationship between Knowledge of Fast Food and Junk Food Consumption and Sociodemographic Characteristics among High School Students in Bogura Town, Bangladesh

Background: The increasing consumption of fast food and junk food among adolescents is a growing public health concern, particularly in urban areas of developing countries like Bangladesh. This study aims to explore the relationship between knowledge of fast food and junk food consumption and sociodemographic characteristics among high school students in Bogura Town, Bangladesh. Methods: This cross-sectional descriptive study was conducted at the Department of Community Medicine, Rajshahi Medical College, from January 2023 to December 2023. A total of 384 high school students from various schools in Bogura were selected using purposive sampling. Data were collected using a pretested semi-structured questionnaire and analyzed using SPSS version 26. Descriptive statistics and chi-square tests were employed to assess associations between variables. Result: The majority of participants were aged 14-16 years (58.07%) and female (66.15%). Most students resided in urban areas (95.31%) and came from nuclear families (87.24%). Parental education levels were high, with 56.77% of fathers and 37.76% of mothers being graduates or having higher education. Despite this, only 23.70% of students had complete knowledge about the nutritional value of fast food and junk food. Significant associations were found between knowledge levels and gender, parental education, economic status, and BMI (p &lt; 0.05). Fast food consumption was prevalent, with 74.48% consuming it at school and 78.13% consuming instant foods at home. Knowledge gaps were notable in areas such as the effects of saturated fats, metabolism, and chronic disease risks. Conclusion: The findings highlight significant gaps in nutritional knowledge among adolescents, despite high rates of fast food consumption. Sociodemographic factors play a crucial role in influencing nutritional knowledge. There is an urgent need for targeted educational programs to address these gaps and promote healthier eating habits among adolescents to improve long-term health outcomes.

Microbial Protein Production Using Lignocellulosic Biomass (Switchgrass) and Klebsiella oxytoca M5A1—A Nitrogen Fixer

The expanding global population has increased the demand for sustainable protein sources, and microbial protein (MP) has emerged as a promising alternative. However, conventional carbon (glucose) and nitrogen (ammonia, urea) sources needed for MP production pose environmental and economic issues. This study aims to produce protein using lignocellulosic biomass (LCB) as a carbon source and the nitrogen fixation ability of Klebsiella oxytoca M5A1 as a nitrogen source. The study investigates the pretreatment of LCB (switchgrass), enzymatic hydrolysis, protein quantification, nitrogen fixation, glucose utilization and organic acids production. K. oxytoca M5A1 harnessed free nitrogen from the atmosphere and used abundant, cheap glucose from LCB to produce MP and organic acids as by-products. Protein production occurred in two phases: first within the initial 8 h and secondly, within the last 16 h. The highest protein concentration was at 40 h, with approximately 683.46 µg/mL protein content. High-performance liquid chromatography system (HPLC) analysis revealed a dynamic profile of glucose utilization and organic acids (Lactic acid, Propionic acid, Acetic acid, and Succinic acid) production. K. oxytoca M5A1 exhibited an early high rate of glucose consumption, and conversion to organic acids, that were later used for second-phase protein production. The acids profile revealed intra-conversion from one acid to another via metabolic pathways (glycolysis and tricarboxylic acid cycle). Overall, leveraging LCB and the nitrogen-fixing ability of K. oxytoca M5A1 for MP production offers an eco-friendly and cost-effective alternative to traditional protein sources, contributing to a sustainable circular economy.