This study aimed to assess the food-related attitudes through the application of a structured questionnaire to 1,000 individuals applied in three environments (groups) in the Federal District of Brazil (supermarkets, universities and hospitals/clinics) using multivariate logistic regression, with special focus on pesticides and genetically modified (GM) food. Outpatients in hospital/clinic, women and older individuals were significantly more likely to adopt diets or attitudes considered or perceived as healthy (including high consumption of fruits and vegetables, acquiring organic food, and adopting procedures to remove pesticide residues from food). When income and/or education impacted the results, the correlation was negative. Over 60% of the population believe it is possible to produce food without using pesticides, mainly the hospital/clinic group, younger individuals, and women, and 95.3% think that the presence of pesticides in food should be indicated on the labels, mainly the hospital/clinic group and older individuals. High worry about pesticides and GM food was associated with most healthy food-related attitudes. The results of this study are important for food-related communication strategies conducted by health authorities, aiming at driving specific population segments to a healthier and more conscious diet.