Pelargoniumsidoides DC is a geophytic species with high demand in the pharmaceutical, aromatherapy, perfumery and cosmetic industries as a result of its unique phytochemistry. The aim of this study was to develop a clonal propagation system for P. sidoides using explants from mature plants, with particular emphasis on the regeneration potential of N6-benzyladenine (BA) and kinetin (KIN) compared to meta-topolin (mT), meta-topolin riboside (mTR) and meta-methoxytopolin riboside (MemTR). Standard colorimetric assays were used to quantify phenolic constituents of the in vitro plants. Cytokinins had a significant effect on shoot regeneration compared to the control. Meta-topolins had significantly higher shoot multiplication and in vitro growth indices compared to both BA and KIN. The highest shoot multiplication indices were obtained at 5.0 μM MemTR >2.0 μM mTR >2.0 μM MemTR >2.0 μM mT. Pelargonium sidoides was intolerant to high BA concentrations as indicated by the low number of shoots per explant (1.0 ± 0.19) at 5.0 μM. Generally, there was a significant increase in phenolic constituents for the CK treatments when compared to the control. Shoot length increased with increasing indole-acetic acid (IAA) and indole-butyric acid (IBA) concentrations whereas the response for α-naphthalene acetic acid (NAA) increased to an optimum then decreased. The highest root biomass was achieved on 1.0 μM IAA >2.0 μM NAA >2.0 μM IBA. The rooting response observed in control plants may be due to the influence of endogenous auxins. In vitro P. sidoides plants were successfully established under ex vitro conditions. In conclusion, meta-topolins were significantly better than BA and KIN in shoot multiplication and promoting in vitro plant growth. The current findings contribute to the increasing research data on the importance of topolins as credible alternatives to traditional CKs in micropropagation.