High-calorie Diet Research Articles

Determination Effects of Rheum ribes L. against High Calorie Diet-induced Obesity: Investigation of Changes in Immunologic and Neurologic Enzymes Activities

This study was carried out to investigate the immunotoxic and neurotoxic effects of Rheum ribes L. (Rr) plant extract on liver, brain and heart tissues in rats with an experimental obesity model. Wistar albino male rats were used in the study. In this study, 4 groups were formed, one of which was the control group, and each group consisted of 6 rats. The groups were the ''control (CG)'', ''high calorie diet (HCD) control (HCDG)'', 'HCD + Rr (200 mg/kg) (HCDRHE1)'' and ''HCD + Rr (400 mg/kg) (HCDRHE2)''. At the end of the study, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), adenosineaminase (ADA) and myeloperoxidase (MPO) biomarkers were evaluated in tissues. According to the study findings, the ADA and MPO activity levels of the obese group increased significantly compared to the control group, and these enzyme levels in the groups administered Rr plant extract approached the control group levels. On the other hand, the AChE and BChE activity levels of the obese group decreased compared to the control group, and there was a statistically significant increase in the groups administered plant extracts compared to the control group. In conclusion, Rr plant root extract suppresses neurotoxicity and immunotoxicity in rats with obesity induced by a high-calorie diet (HCD).

Cardio protective effect of intermittent fasting on the lumen area ratio of the coronary artery in rats with a high-calorie diet

Atherosclerosis is a principal cause of coronary artery disease (CAD), one of the major causes of global premature mortality and morbidity. The glycolysis pathway will process excess carbohydrates in a high-calorie diet (HCD) into triglycerides, which, if accumulated, will cause an increase in the concentration of atherogenic triglyceride-rich lipoproteins. Therefore, intermittent fasting (IF) is a recommended lifestyle to prevent CAD through metabolic reprogramming pathways. This study was designed to evaluate the cardioprotective effect of eight weeks of IF 5:2 on the lumen area ratio of the coronary artery in rats with four weeks of HCD. A total of 24 Wistar male albino rats were divided into SD (standard diet), IF (IF 5:2), HCD (daily injection 0.013 g/gBW glucose), and IF-HCD (IF 5:2 and HCD). At the end of the experiment, all the rats were sacrificed. The coronary artery preparations were stained with H&E stain, then evaluated with the OLYMPUS cellSens Standard to measure the arterial lumen area. The statistical analysis was done using Brown Forsythe test. In this study, no significant differences between all groups, and the effect of IF will only be seen if an HCD intervention has a significant effect. However, the IF group achieved the highest mean in the arterial lumen area ratio (SD=0.526±0.097; IF=0.631±0.021; HCD=0.611±0.064; IF-HCD=0.594±0.060). In conclusion, IF 5:2 may have a cardioprotective effect in healthy individuals, but the effect is unknown in individuals with HCD.

Cardio protective effect of intermittent fasting on the lumen area ratio of the coronary artery in rats with a high-calorie diet

Cardio protective effect of intermittent fasting on the lumen area ratio of the coronary artery in rats with a high-calorie diet

Intake of slow-digesting carbohydrates is related to changes in the microbiome and its functional pathways in growing rats with obesity induced by diet.

The main cause of insulin resistance in childhood is obesity, which contributes to future comorbidities as in adults. Although high-calorie diets and lack of exercise contribute to metabolic disease development, food quality rather than the quantity of macronutrients is more important than food density. The purpose of the present study was to examine the effects of changing the quality of carbohydrates from rapidly to slowly digestible carbohydrates on the composition of the gut microbiota and the profiles of the functional pathways in growing rats with obesity due to a high-fat diet (HFD). During the course of 4 weeks, rats growing on an HFD-containing carbohydrates with different digestive rates were fed either HFD-containing carbohydrates with a rapid digestion rate (OBE group) or HFD-containing carbohydrates with a slow digestion rate (OBE-ISR group). A non-obese group (NOB) was included as a reference, and rats were fed on a rodent standard diet (AIN93G). An analysis of gut microbiota was conducted using 16S rRNA-based metagenomics; a linear mixed-effects model (LMM) was used to determine changes in abundance between baseline and 4 weeks of treatment, and functional pathways were identified. Gut microbiota composition at bacterial diversity and relative abundance, at phylum and genus levels, and functional profiles were analyzed by integrating the Integrated Microbial Genomes (IMG) database. The groups showed comparable gut microbiota at baseline. At the end of the treatment, animals from the ISR group exhibited differences at the phylum levels by decreasing the diversity of Fisher's index and Firmicutes (newly named as Bacillota), and increasing the Pielou's evenness and Bacteroidetes (newly named as Bacteroidota); at the genus level by increasing Alistipes, Bifidobacterium, Bacteroides, Butyricimonas, Lachnoclostridium, Flavonifractor, Ruminiclostridium 5, and Faecalibaculum and decreasing Muribaculum, Blautia, and Ruminiclostridium 9. Remarkably, relative abundances of genera Tyzzerella and Angelakisella were higher in the OBE group compared to NOB and OBE-ISR groups. In addition, some microbiota carbohydrate metabolism pathways such as glycolysis, glucuronic acid degradation, pentose phosphate pathway, methanogenesis, and fatty acid biosynthesis exhibited increased activity in the OBE-ISR group after the treatment. Higher levels of acetate and propionate were found in the feces of the ISR group compared with the NOB and OBE groups. The results of this study demonstrate that replacing rapidly digestible carbohydrates with slowly digestible carbohydrates within an HFD improve the composition of the gut microbiota. Consequently, metabolic disturbances associated with obesity may be prevented.

Review of literature: Etiology of obesity, risk factors, and the role of genetics

Obesity is considered one of the global most significant public problem as it has been associated with different diseases and even death. It is an excess accumulation of fat in the body as a result of increase calorie intake compared to energy expenditure. It is considered as multifactorial condition as many factors play role in its occurrence including genetic susceptibility, external environmental factors, individual attitude. The exact etiology of obesity is intermingled with different factors and nowadays because of development of different aspects of life, sedentary life style dominates the social communities, reduced physical activities, all these factors can enhance the risk of obesity. Furthermore, disturbances of the endocrine system like Cushing’s syndrome, hypothyroidism, diabetes type II. The genetic obesity it will be divided into two groups: 1) The monogenic type, the Poly genic type, syndrome associated obesity. Many individuals are suffering from obesity because of different genes that they already have which encourage them to high calorie diet with more food consumption, unlimited and uncontrolled food intake together with reduced physical activity. In conclusion the etiology, risk factors, as well as genetic susceptibility all are combined and correlated to each other as they may act separately or together to induce onset of obesity. The physical exercise and energy expenditure are having the key role., in addition to endocrine hormonal disturbances.

Effect of β-sitosterol on insulin resistance & protein expression of insulin signalling molecules in quadriceps muscle of high fat diet-induced type-2 diabetic rats.

Changes in life style, such as high-calorie diet intake and lack of exercise, have increased the global prevalence of obesity and diabetes. The major pathophysiological event contributing to the development of type 2 diabetes mellitus is the target tissues to insulin action. Currently available drugs are unsuccessful for the treatment of type 2 diabetes due to their unwanted side effects. Hence, search drugs, from plant sources. β-sitosterol is plant sterols with structurally almost like that of cholesterol. It is widely present in various medicinal plants. It has been reported to elicit multitude of bioactivities including anti-lipidemic and anti-hyperglycemic activity. However, specific effect of β-sitosterol on insulin signalling molecules of quadriceps muscle remains unclear. Hence, the study aimed to assess the beneficial role of β-sitosterol on the expression of insulin signalling molecules in quadriceps muscle of high fat diet and sucrose-induced type-2 diabetic rats. The oral effective dose of β-sitosterol (25 mg/kg body weight) was given once each day until the end of the study (30 days post‑induction of diabetes) to HFD- fed diabetic rats. At the end of the experimental period, fasting blood sugar (FBG), oral glucose (OGTT) and insulin tolerances (IT), Tissue glycogen, Serum insulin, Homeostasis Model Assessment for Insulin Resistance (HOMA-IR) and Quantitative Insulin Sensitivity Check Index (QUICKI), as well as the levels of insulin signalling molecules like insulin receptor (IR), glucose transporter subtype 4 (GLUT4) proteins and glycogen concentration within the quadriceps muscle were assessed. A diabetic rat indicates impaired glucose and insulin tolerances and insulin signalling molecules (IR and GLUT4) proteins and glycogen concentration. Serum insulin, were found to be increased in diabetic rats. The treatment with β-sitosterol normalized the altered levels of blood glucose, serum insulin, and IR and GLUT4 protein levels. Thus, we concluded that β-sitosterol enhances glycemic control through activation of IR and GLUT4 in the quadriceps muscle of high fat diet and sucrose‑induced type2 diabetic rats.

Influence of mechanical stimuli and zoledronic acid on the femoral morfology in rats with obesity and limited mobility models

The aim. Investigate the long-term effect of WBV and bisphosphonate (zoledronic acid) on the morphological structure and mineral density of the rat femora. Materials and methods. Rats were divided into control and three experimental groups: I - experimental group - high-calorie diet + limited mobility (HCD + LM), II - experimental group - HCD + LM + whole body vibration (WBV 3 m/s2). III - experimental group - HCD + LM + administration of zoledronic acid (HCD + LM + Zol. at a dose of 0.025 mg / kg). Bone mineral density determination, scanning electron microscopy of trabecular meshwork and immunohistological examination were performed. Results. The application of the model of a high-calorie diet with limited mobility leads to the loss of the mineral component of trabeculae up to 16.2%. The trabecular layer thinned to 13.9% compared to the control group, and the intertrabecular spaces expanded. Under the influence of whole-body vibration, there was an increase in BMD in the femora compared to the group HCD + LM, and bone density remained at the control level until the 16th week of the experiment. In group III, the rate of bone formation increased, the ratio of bone to trabecular volume was significantly higher compared to the group HCD + LM. Starting from the 8th week, it increased by 8.1% and 13% at the end of the experiment, respectively.Conclusions. A high-calorie diet with limited mobility reduces the mineral density of the trabeculae in the femoral bone. Zoledronic acid effectively improves mineralization but leads to impairment of the morphology of the bone growth plate. The research results indicate that WBV can be an effective non-pharmacological method of maintaining bone health in young people.

The effect of food on the pharmacokinetics of WXFL10203614, a potential selective JAK1 inhibitor, in healthy Chinese subjects.

Objective: This study was performed to investigate the effect of food on the pharmacokinetics (PK) of WXFL10203614 in healthy Chinese subjects. Methods: This was a randomized, open-label, single-dose, two-treatment (fed vs fasted), two-period, two-sequence, crossover study. 14 eligible subjects were averagely randomized into 2 sequences and then received 10mg WXFL10203614 under fasted or fed condition. In each period, the blood samples were collected from 0h (pre-dose) and serially up to 72h post-dose, and plasma concentrations were detected using the high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method. The effect of food on the PK profile and safety of WXFL10203614 were assessed. Results: 70 subjects were screened, and 14 subjects (10 male and 4 female) were enrolled and completed the study. Under the fasted condition, WXFL10203614 was absorbed rapidly with a Tmax of 0.98h. The absorption rate was slower, Tmax delayed by 2.98h, and the Cmax decreased by 16.3% when WXFL10203614 administered after the high-fat and high-calorie diet, other PK parameters were not affected. The 90% confidence intervals (CIs) for the ratio (fed/fasted) of geometric means of the Cmax, AUC0-t and AUC0-∞ were 0.73-1.01, 0.90-1.03 and 0.90-1.03, indicating that the high-fat and high-calorie diet might impact the absorption process of WXFL10203614. Although the Cmax was slightly decreased, there was no significant difference in the Cmax under fasted and fed conditions. Thus, it was not considered clinically significant owing to the small magnitude of changes in Cmax. All Treatment-emergent adverse events (TEAEs) were mild and resolved spontaneously without treatment. Conclusion: Food had no clinically relevant effects on drug system exposure of WXFL10203614. It was well tolerated under fasted and fed conditions in healthy Chinese subjects, so WXFL10203614 could be administered orally with or without food. Clinical Trial Registration: http://www.chinadrugtrials.org.cn/index.html, identifier CTR20191636.

Adolescent growth and convict transportation to nineteenth-century Australia

ABSTRACT This paper explores growth patterns for British and Irish adolescents transported to Australia in the 19th century. During incarceration in Australia, the young convicts did not catch up with contemporary standards of potential stature—contrary to what we are led to expect by the existing literature and the high calorie convict diet. Rather, the experience of transportation stunted the adolescent male convicts. Variation between height on arrival and in later life confirms that teen convicts remained shorter than their shipmates transported after reaching maturity. We consider, but reject, age-dependent selection as a potential explanation. We speculate that the origin of this unfortunate experience lies in the low economic value of young and unskilled males. While fewer data are available for female convicts, their colonial experiences appear to have differed, again consistent with their relative economic value in the colony.

Combined spectroscopic, biochemical and chemometric approach toward finding of biochemical markers of obesity

BackgroundEarly-stage detection of subclinical obesity-driven systemic changes is a challenging area of medical diagnostics, where the most popular existing measures – such as body mass index – BMI – often fall short of providing a realistic estimate of adiposity and, therefore, of ongoing pathologies at the systemic, tissue and cellular level. In the quest for identifying new more robust diagnostic markers, whole-organ analysis of chemical elements is a promising approach for identifying candidate proxies of obesity status in the system. MethodsTotal Reflection X-ray fluorescence (TXRF) coupled with biochemical assays, chemometrics and statistical validation was used as a new integrated pipeline for marker identification in external ear samples of obese animals. The specimens were taken from obese animals fed a high calorie diet as well as from lean intact animals fed a standard diet. ResultsThe most significant differences in the content of K, Fe, Br, and Rb between the studied groups of the animals were identified. However, with the methodology applied Rb was found the most robust biochemical discriminator of early-stage obesity effects, as validated by the logistic regression model. We observed no relationship between the levels of the elements consumed by the animals and their apparent content in the earlobe tissue samples. ConclusionsOur preliminary study confirms that obesity alters tissue trace metal metabolism and shows the proposed new approach as an accurate and reliable methodology for detecting tissue elemental obesity-related alterations. General significanceThis result can be of practical significance for designing new point-of-care systems for obesity screening tests, taking advantage of direct/indirect Rb measurements.

Moderate intensity continuous and interval training affect visceral fat and insulin resistance model in female rat exposed high calorie diet

Chronic high-calorie diet (HCD) combined with physical inactivity promotes obesity and insulin resistance (IR). This study aimed to analyse the comparable effect of moderate-intensity continuous training (MICT) and moderate-intensity interval training (MIIT) on visceral fat weight and IR in subjects exposed to HCD. This randomised post-test research used only a control group design with female rats (Wistar norvegicus), 8 weeks old and 100-200 g of bodyweight. They were randomly divided into four groups: standard diet group (C), HCD group (C1), HCD combined with MICT group (C2) and HCD combined with MIIT group (C3). Each group consisted of six rats. HCD consisted ofad libitumstandard diet plus dextrose solution by oral gavage for 4 weeks. The MICT was conducted by swimming plus 6% load of body weight for 10 min in the first week, for 20 min in the second week and 30 min in the third and fourth week. The MIIT was conducted by swimming in a ratio between swimming and rest time at 2:1 plus 6% load of BW, performed 5×/week for 4 weeks, and increased progressively. The mean body weight pre-intervention was 152.79±13.280 g and 150.12±9.195 g post-intervention (P=0.115). The mean fasting blood glucose pre-intervention was 79±8.668 mg/dl, and post-intervention 86.29±12.142 mg/dl (P=0.142). The mean visceral fat weight between C (1.94±0.66 g), C1(1.45±0.47 g), C2(1.41±0.44 g), and C3(1.22±0.59 g) was not significant (P=0.179). The mean triglyceride level for C (173.33±30.30 mg/dl), C1(157.16±47.32 mg/dl), C2(112.83±25.49 mg/dl), and C3(80.33±23.47 mg/dl) was significant (P=0.000). The mean IR model for C (4.796±0.070), C1(4.728±0.125), C2(4.620±0.123), C3(4.360±0.143) was significant (P=0.000). In conclusion, both MICT and MIIT have an effect to improve IR and TG. The MIIT was more effective to improve IR compared to MICT in the female rats exposed to an HCD.

Correlation between lifestyle patterns and overweight and obesity among Chinese adolescents.

Lifestyles such as physical exercise, sedentary behavior, eating habits, and sleep duration are all associated with adolescent overweight and obesity. The purpose of this study was to investigate how Chinese adolescents' lifestyles clustered into different lifestyle patterns, and to analyze the correlation between these patterns and adolescent overweight and obesity. The investigated respondents included 13,670 adolescents aged 13-18 from various administrative regions in China. Latent class analysis was employed to cluster the lifestyles of adolescents, χ2 test and Logistic regression were used to explore the relationship between lifestyle patterns and overweight and obesity in adolescents. The results identified 6 types of Chinese adolescents' lifestyle patterns, as well as the significant differences in gender and age. The adolescents with high exercise-high calorie diet had the lowest risk of overweight and obesity, and the adolescents with low consciousness-low physical activity and low consciousness-unhealthy had the highest risk of overweight and obesity, which were 1.432 times and 1.346 times higher than those with high exercise-high calorie diet, respectively. The studied demonstrated that there was a coexistence of healthy behaviors and health-risk behaviors in the lifestyle clustering of Chinese adolescents. Low physical exercise and high intake of snacks and carbonated beverages were the most common. Physical exercise and health consciousness were the protective factors of overweight and obesity in adolescents.

Effect of empagliflozin on cytoskeletal repair in the hippocampus of obese mice.

We aimed to investigate the effect of empagliflozin on hippocampal phosphorylated protein levels in obese mice. Sixteen obese mice successfully modeled on high-fat diet were randomly divided into high-fat feeding group (group H) and empagliflozin group (group H + empagliflozin, group E), eight mice in each group, and eight C57BL/6J male normal mice were selected as the control group (normal control, group C). Group E was treated with empagliflozin 10 mg/kg/d for 12 weeks, while mice in groups H and C were treated with equal amounts of saline. The spatial learning memory ability of the mice was determined by the Morris water maze experiment. Further, their body weights and serological indices were measured. Finally, total proteins were extracted from hippocampal tissues for functional analysis by the phosphorylated proteomics method. The results showed that escape latency was prolonged, retention time in the target quadrant was shortened, and the number of loop penetrations was reduced in the obese mice induced by a high-calorie diet compared with normal controls, whereas escape latency was shortened, retention time in the target quadrant was increased, and the number of loop penetrations was increased after empagliflozin treatment. Phosphoproteomics in the high-fat/control (H/C), empagliflozin/high-fat (E/H), and E/C groups showed 844, 1,552, and 1,512 differentially significant phosphorylation sites, respectively. The proteins corresponding to these differentially phosphorylated sites were mainly involved in neurodegenerative pathways and actin cytoskeleton regulation. Notably, myosin heavy chain 10 (MYH10), p21 protein-activated kinase 4 (PAK4), phosphatidylinositol 3 -phosphate 5-kinase (PIKfyve), and other differentially phosphorylated proteins were involved in actin cytoskeleton regulation. We concluded that empagliflozin protects cognitive functions by inducing serine phosphorylation in MYH10, PAK4, and PIKfyve in the hippocampal tissue of obese mice.

PSUN280 A Rare Genetic Disorder With Recurrent Hypoglycemic Episodes and Ketonemia Requiring Multidisciplinary Team Management During Pregnancy

Abstract Introduction Beckwith-Wiedemann syndrome (BWS; OMIM 130650) is a multisystem human genomic imprinting disorder of chromosome 11p15.5. It is characterized by overgrowth and cancer susceptibility and is known to have a highly variable clinical spectrum. The main features include lateralized overgrowth and hypoglycemia due to prolonged hyperinsulinism. Most case reports on BWS are focused on the pediatric population with very scarce data in adults. Clinical case A 29-year-old, paralympic swimmer and genetically diagnosed female with BWS had recurrent episodes of hypoglycemia during her second pregnancy. She had a history of six previous first trimester miscarriages, anti-phospholipid syndrome, hypoglycaemic seizures, asthma, and migraines. Pre-pregnancy, she was investigated for a neurological cause of her seizures and an electroencephalogram and brain imaging were unremarkable. She underwent extensive investigations for evaluation of hypoglycemia and was diagnosed as having hyperinsulinemia secondary to pancreatic hypertrophy. Factitious hypoglycemia was also ruled out. Tumor localization studies were all negative. A supervised fast could not be conducted due to the inability of the patient to tolerate the test. Her first viable pregnancy was 2 years back, during which she required a prolonged hospital (including the intensive care unit) admission with multiple in-hospital complications and requiring enteral feeding. During her current pregnancy, she experienced recurrent episodes of severe disabling hypoglycemia resulting in seizures. She was commenced on diazoxide, however, despite that, she continued to have hypoglycemic episodes for which she had to self-inject Glucagon several times. She also had unexplained weight loss with ketonemia undeterred by a high-caloric diet. She refused to be on naso-jejunal feeding or enteral feeding. She self funded a flash glucose monitor which recorded her blood glucose levels between 2.0 to 3.4 mmol/L during the episodes of hypoglycemia. Dietitians were continually involved in her care to provide nutritional support and to optimize blood glucose control through dietary manipulation. Conclusion This was a very interesting and complicated case of a genetic disorder that presented multiple challenges during pregnancy. Management focused on psychological concerns, the severe catabolic state leading to rapid weight loss, recurrent hypoglycemic episodes, ketonemia, nutritional support, and fetal well-being monitoring. A multi-disciplinary team approach to management involved the obstetrics, endocrinology, dietetics, and gastroenterology teams. This resulted in a successful outcome for her pregnancy. Very limited data is available on health issues associated with BWS in adulthood and there is no data available on the obstetric management of women with BWS. Additionally, the unexplained weight loss and ketonemia associated with hypoglycemia make this case unique. Further studies are warranted and national guidelines are required to ensure that women with BWS achieve optimum pregnancy outcomes. Presentation: Sunday, June 12, 2022 12:30 p.m. - 2:30 p.m.

The Impact of a Low-Normal Protein High Calorie Diet in the Onco-Nephrological Scenario: Do the Opposites Attract?

The Impact of a Low-Normal Protein High Calorie Diet in the Onco-Nephrological Scenario: Do the Opposites Attract?

Inhibition of farnesyl pyrophosphate synthase alleviates cardiomyopathy in diabetic rat

ABSTRACT This study investigated the effects of ibandronate (IBN) on cardiomyopathy remodeling in diabetic rats. A rat model of diabetic cardiomyopathy (DCM) was established by supplementing them with a high-calorie diet combined with a low dose of streptozotocin (STZ). The diabetic rats received IBN (5 µg/kg per day) or normal saline subcutaneously for 16 weeks. The hematoxylin and eosin (H&E) and Masson’s trichrome staining were performed for evaluating the myocardial morphologies of the rats. Echocardiography and cardiac catheter were performed to assess their cardiac functional parameters. The protein levels of connective tissue growth factor (CTGF), farnesyl pyrophosphate synthase (FPPS), and mitogen-activated protein kinase (MAPK) were determined using Western blot analysis. RhoA activation was detected using a small GTP protease-linked immunosorbent assay (GLISA). The diabetic rats showed the development of moderate hyperglycemia, insulin resistance, hyperlipidemia, myocardial fibrosis, FPPS overexpression, cardiac systolic, and diastolic dysfunction. Inhibiting the FPPS could ameliorate myocardial hypertrophy and fibrosis. These anatomical findings were accompanied by a significant improvement in heart function. Furthermore, the inhibition of FPPS, the increased activation of RhoA, and phosphorylation of p38 and extracellular signal-regulated kinase (ERK)1/2 in DCM decreased significantly with the treatment of IBN. This study for the first time demonstrated that the upregulation of FPPS expression might be involved in diabetic myocardial remodeling in diabetes mellitus (DM). In addition, IBN might exert its inhibitory effects on myocardial tissue remodeling by suppressing the RhoA/ERK1/2 and RhoA/p38 MAPK pathways in DCM.

Histomorphological features of the thyroid gland in rats of different ages with alimentary-induced obesity

The aim of the study was to compare histomorphological disorders of the thyroid gland in rats of different ages with alimentary-induced obesity. The study was carried out on male Wistar rats, aged six and 21 months. Alimentary obesity in animals was modelled by keeping them on a high-calorie diet (580 kcal 100 g‑1) for 12 weeks, with an excess content of fats and carbohydrates. Control animals were given a standard diet (330 kcal 100 g‑1). Histological preparations were made from thyroid tissue. Histomorphometry was carried out using the computer program “ImageJ”. It was revealed that a 3‑month high-calorie diet led to the appearance of pronounced histomorphological signs of thyroid hypofunction in rats. Intensive colloid resorption and a decrease in colloid area occurred in the gland. Devastated follicles were common. Thyrocytes acquired a prismatic shape. There was hyperplasia of the thyroid gland, which led to an increase in its size. The intensity of morphological disorders of the thyroid gland depended on the degree of obesity and had a distinct age-dependent character. In young rats, these changes were more pronounced than in older animals.

Association of neutrophil gelatinase associated lipocalin, ischemia modified albumin with uric acid in the etiopathogenesis of preeclampsia.

Preeclampsia is a multisystem illness that manifests in the third trimester of pregnancy after 20weeks of gestation and is marked by proteinuria and hypertension (PE). Changes in lifestyle, such as eating a high-calorie dietand delaying delivery, have raised the likelihood of developing PE. Eclampsia, abrupt renal failure, thromboembolic episodes leading to cardiac and brain problems, pulmonary embolism, and coagulopathy associated with HELLP syndrome are a few of the complications that might follow preeclampsia in pregnant moms. The objects of this study is to estimate and correlate the levels of NGAL (neutrophil gelatinase associated lipocalin), IMA (ischemia modified albumin) and Uric acid in prreclampsia. 40 diagnosed cases of preeclampsia and 40 healthy age and gestational age matched healthy controls were included in the study. Blood samples were collected from them and serum NGAL, IMA and Uric acid levels were estimated. Estimation of NGAL (neutrophil gelatinase associated lipocalin), IMA (ischemia modified albumin) was done by commercially available ELISA kits standard spectrophotometry methods in autoanalyzer Mind ray BS300 using commercially available kits. The parameters of NGAL and IMA were significantly increased in patients with PE (p<0.001) when compared with the healthy control subjects. γ-glutamyl transferases and OPN were found in patients with ALD (p<0.001) when compared with the control subjects. OPN showed significant positive correlations with AST (r=0.76, p<0.001), ALT (r=0.64 p<0.001), ALP (r=0.68, p<0.001), and GGT (r=0.61, p<0.001). The current study focuses on the roles of NGAL and IMA, two sensitive markers of kidney injury thatare particularly useful in identifying widespread endothelial dysfunction. As a result, the pattern of elevated NGAL and IMA levels can be useful for diagnosis.

Cannabinoid CB1 Receptor Deletion from Catecholaminergic Neurons Protects from Diet-Induced Obesity.

High-calorie diets and chronic stress are major contributors to the development of obesity and metabolic disorders. These two risk factors regulate the activity of the sympathetic nervous system (SNS). The present study showed a key role of the cannabinoid type 1 receptor (CB1) in dopamine β-hydroxylase (dbh)-expressing cells in the regulation of SNS activity. In a diet-induced obesity model, CB1 deletion from these cells protected mice from diet-induced weight gain by increasing sympathetic drive, resulting in reduced adipogenesis in white adipose tissue and enhanced thermogenesis in brown adipose tissue. The deletion of CB1 from catecholaminergic neurons increased the plasma norepinephrine levels, norepinephrine turnover, and sympathetic activity in the visceral fat, which coincided with lowered neuropeptide Y (NPY) levels in the visceral fat of the mutant mice compared with the controls. Furthermore, the mutant mice showed decreased plasma corticosterone levels. Our study provided new insight into the mechanisms underlying the roles of the endocannabinoid system in regulating energy balance, where the CB1 deletion in dbh-positive cells protected from diet-induced weight gain via multiple mechanisms, such as increased SNS activity, reduced NPY activity, and decreased basal hypothalamic-pituitary-adrenal (HPA) axis activity.

High-Density Lipoprotein Cholesterol as a Potential Medium between Depletion of Lachnospiraceae Genera and Hypertension under a High-Calorie Diet.

Gut microbial dysbiosis has been associated with hypertension. An extremely high incidence of essential hypertension was found in the Han and the Yugur people who resided in Sunan County in China's nomadic steppes, with little population movement. To investigate gut microbial contributions to this high incidence of hypertension, we recruited a total of 1, 242 Yugur and Han people, who had resided in Sunan County for more than 15 years and accounted for 3% of the local population. The epidemiological survey of 1,089 individuals indicated their nearly 1.8-times-higher prevalence of hypertension (38.2 to 43.3%) than the average in China (23.2%), under a special high-calorie diet based on wheat, cattle, mutton, and animal offal. Investigations of the fecal microbiota of another cohort of 153 individuals revealed that certain Lachnospiraceae genera were positively correlated with high-density lipoprotein cholesterol (HDL-C) but negatively correlated with systolic blood pressure (SBP) and diastolic blood pressure (DBP). HDL-C was negatively correlated with SBP and DBP. We further observed that the serum butyrate content was lower in both Han and Yugur people with hypertension than in those without hypertension. This study gives novel insight into the role of gut microbial dysbiosis in hypertension modulation under a high-calorie diet, where the notable depletion of Lachnospiraceae genera might lead to less production of butyrate, contributing to the lower level of HDL-C and elevating blood pressure in hypertension. IMPORTANCE Dietary nutrients can be converted by the gut microbiota into metabolites such as short-chain fatty acids, which may serve as disease-preventing agents in hypertension. Due to the limited population mobility and unique high-calorie dietary habits, the cohort of this study can serve as a representative cohort for elucidating the associations between the gut microbiota and hypertension under a high-calorie diet. Moreover, low levels of HDL-C have previously been associated with an increased risk of various cardiovascular diseases (CVDs). Our findings provide new insight showing that low levels of HDL-C may be a potential medium between the depletion of Lachnospiraceae genera and hypertension under a high-calorie diet, which might also be a potential candidate for other CVDs.