High Biomass Research Articles

Stem cells of Impatiens tinctoria A.Rich tuber and antioxidant activity of their extracts

The use of plant stem cell extracts as active ingredients in cosmetic formulations has gained popularity recently, highlighting the need to establish Impatiens tinctoria A. Rich in vitro cultures in order to prepare bioactive stem cell extracts. This study is aimed at establishing cell suspension culture and evaluates the antioxidant activity of the stem cell extract. In order to initiate callus and cell suspension cultures, tuber explants were inoculated onto Murashige and Skoog (MS) media containing 2,4-D (0.5–2 mg/L) and BAP (0–2 mg/L). Optimal hormone concentrations of 2 mg/L 2,4-D and 1.5 mg/L BAP were sufficient to produce callus. The obtained callus was utilized as the inoculum to start a cell suspension culture for the production of stem cell extracts. High biomass accumulation was obtained at 30 g/L sucrose concentration and 6g inoculum size. The stem cell extract had a total phenolic content of 4.6 µgGAE/mL and a flavonoid content of 190.96 µgQE/mL. DPPH scavenging activity of 95.82% and the IC50 value of 37.54 µg/mL was detected for the stem cell extract. The study indicated that the suspension cultures of I. tinctoria A. Rich have the potential to produce stem cell extracts with increased flavonoids content and antioxidant activity.

Epibiotic Bacteria Isolated from the Non-Indigenous Species Codium fragile ssp. fragile: Identification, Characterization, and Biotechnological Potential.

Due to their richness in organic substances and nutrients, seaweed (macroalgae) harbor a large number of epiphytic bacteria on their surfaces. These bacteria interact with their host in multiple complex ways, in particular, by producing chemical compounds. The released metabolites may have biological properties beneficial for applications in both industry and medicine. In this study, we assess the diversity of culturable bacterial community of the invasive alga Codium fragile ssp. fragile with the aim to identify key groups within this epiphytic community. Seaweed samples were collected from the Northern Tunisian coast. A total of fifty bacteria were isolated in pure culture. These bacterial strains were identified by amplification of the ribosomal intergenic transcribed spacer between the 16S and the 23S rRNA genes (ITS-PCR) and by 16S rRNA sequencing. Antimicrobial activity, biochemical, and antibiotic resistance profile characterization were determined for the isolates. Isolated strains were tested for their antimicrobial potential against human and fish bacterial pathogens and the yeast Candida albicans, using the in vitro drop method. About 37% of isolated strains possess antibacterial activity with a variable antimicrobial spectrum. Ba1 (closely related to Pseudoalteromonas spiralis), Ba12 (closely related to Enterococcus faecium), and Bw4 (closely related to Pseudoalteromonas sp.) exhibited strong antimicrobial activity against E. coli. The isolated strain Ba4, closely related to Serratia marcescens, demonstrated the most potent activity against pathogens. The susceptibility of these strains to 12 commonly used antibiotics was investigated. Majority of the isolates were resistant to oxacillin, cefoxitin, tobramycin, and nitrofurantoin. Ba7 and Ba10, closely related to the Vibrio anguillarum strains, had the highest multidrug resistance profiles. The enzymes most commonly produced by the isolated strains were amylase, lecithinase, and agarase. Moreover, nine isolates produced disintegration zones around their colonies on agar plates with agarolitic index, ranging from 0.60 to 2.38. This investigation highlighted that Codium fragile ssp. fragile possesses an important diversity of epiphytic bacteria on its surface that could be cultivated in high biomass and may be considered for biotechnological application and as sources of antimicrobial drugs.

Oil palm frond leaves (OPFLs), a high recalcitrant biomass as an alternative cellulose source for glucose conversion

Among the oil palm biomass, oil palm frond leaves (OPFLs) are under-valued since they can be classified as a high recalcitrance feedstock due to the high lignin and hemicellulose content. This work proposes a new way of using OPFLs as alternative cellulose sources for glucose conversion by an application of various pre-treatments at varying parameters. From green pre-treatment, steam explosion (SE) is much more effective in disrupting the OPFLs structure in contrast to hydrothermal (HT) pre-treatment, by a vast dissolution of hemicellulose and depolymerization of lignin. The pre-impregnation with H2SO4 prior to steam explosion was beneficial to enhance the hemicellulose removal up to 89.94 %. The 2D-HSQC NMR analysis divulged the reduction of ß-O-4 linkage and S/G value after the SE and HT pre-treatment, verifying the degradation of lignin-carbohydrate linkage in OPFLs. Meanwhile, the dilute NaOH pre-treatment enhanced the delignification by 87.14 %. SEM analysis revealed a well-disrupted structure of pre-treated OPFLs exposing a higher cellulosic fraction, whereas SEC analysis divulged a decreasing pattern of degree of polymerization of cellulose. The enzymatic analysis revealed that pre-treated OPFLs yielded higher glucose ranging from 29.81 % to 49.98 % w/w, which was ∼3-fold higher than raw OPFLs (14.31 % w/w). A maximum cellulose digestibility (100 %) was achieved by steam-exploded OPFLs after 72 h of enzymatic hydrolysis. This suggests the potency of various pre-treatments to enhance enzymatic saccharification of OPFLs producing a higher glucose yield.

Grassland nature reserves safeguard a high species richness and biomass of grasshoppers

Grassland nature reserves safeguard a high species richness and biomass of grasshoppers

Comparative transcriptome and coexpression network analysis revealed the regulatory mechanism of Astragalus cicer L. in response to salt stress

BackgroundAstragalus cicer L. is a perennial rhizomatous legume forage known for its quality, high biomass yield, and strong tolerance to saline-alkaline soils. Soil salinization is a widespread environmental pressure. To use A. cicer L. more scientifically and environmentally in agriculture and ecosystems, it is highly important to study the molecular response mechanism of A. cicer L. to salt stress.ResultsIn this study, we used RNA-seq technology and weighted gene coexpression network analysis (WGCNA) were performed. The results showed 4 key modules were closely related to the physiological response of A. cicer. L. to salt stress. The differentially expressed genes (DEGs) of key modules were mapped into the KEGG database, and found that the most abundant pathways were the plant hormone signal transduction pathway and carbon metabolism pathway. The potential regulatory networks of the cytokinin signal transduction pathway, the ethylene signal transduction pathway, and carbon metabolism related pathways were constructed according to the expression pathways of the DEGs. Seven hub genes in the key modules were selected and distributed among these pathways. They may involved in the positive regulation of cytokinin signaling and carbon metabolism in plant leaves, but limited the positive expression of ethylene signaling. Thus endowing the plant with salt tolerance in the early stage of salt stress.ConclusionsBased on the phenotypic and physiological responses of A. cicer L. to salt stress, this study constructed the gene coexpression network of potential regulation to salt stress in key modules, which provided a new reference for exploring the response mechanism of legumes to abiotic stress.

Grain productivity and nutrient accumulation in millet straw grown in the off-season

Given the growing demand for grains cultivation in the off-season, an experiment was conducted to evaluate grain yield and nutrient accumulation in straw, straw production, and decomposition of pearl millet varieties and hybrids cultivated at different sowing times in off-season in the municipality of Rio Verde, state of Goiás. This was a randomized block experimental design, with four replications and four planting times. The work was carried out on an Oxisol, using two forage varieties, ADR300 and ADR500, the dual-purpose pearl millet hybrid ADR8010 for grain and biomass production, and two grain hybrids, ADR9010 and ADR 9020. The accumulation of macronutrients (nitrogen, phosphorus, potassium, calcium, magnesium and sulfur) was quantified in the straw at grain harvest. Hybrids ADR 9010 and ADR 9020 had the highest grain yield in the first season, with 2,626 and 2,425 kg ha-1, respectively, while the ADR 9010 hybrid showed the highest accumulation of macronutrients (except phosphorus) in the straw. Hybrid ADR 9010 is the best option for planting in the off-season with the objective of grain production, with high biomass production and accumulation of nutrients in the straw.

In Planta Androgenesis (Paternal Origin) in Saacharum X Erianthus Intergeneric Hybrid

The present investigation was carried in crosses made between Saccharum species hybrid clones i.e. Co 419, Co 7201 and Co 7224 (2n = 108-112) and Erianthus arundinaceus viz. IK 76-91 (2n = 60) and IK 76-99 (2n = 60) for introducing better ratoon ability, vigour, wide ecological adaptability, extensive root system, drought and water logging resistance, high biomass and disease resistance into modern sugarcane cultivars during 2020-23 at VSI-Sugarcane Breeding Centre, Amboli. Parental apomixis (paternal origin) has been recently noticed and it is unique in Saccharum X Erianthus intergeneric cross seedlings. A total of fifty-one seedlings were obtained from above three intergeneric crosses and out of these eighteen seedlings were reported as androgenetic seedlings as the female act as a surrogate mother. At the early stages, seed germination of seedlings was found two types i.e., one type resembling Saccharum and other resembling Erianthus with deep purple coloration. Morphologically androgenetic seedlings were similar to the male parents IK 76-91 and IK 76-99 with tall large tufted grass like clumps, vegetative stem with thick reproductive buds, root zone narrow and only one row of root eyes, the lamina gradually passes into leaf sheath with no auricle and dewlaps. Cytological studies were made in these seedlings and during metaphase the somatic chromosome number was observed to be 2n = 60 rather than expected 2n = 84-90. This paternal origin may be due to the ability of Erianthus male gametes to produce an embryo within the seed tissues of Saccharum species hybrids but without genetic contribution of the seed. Thus, in planta androgenesis is achieved through the combination of embryogenic behavior of male gametes which has entered in the ovule and the ability of ovules to act as a surrogate mother. They could derive either from the fusion of two male gametes or from the early natural diplodization of the haploid embryo. Such homozygous true breeding seedlings of Saccharum X Erianthus with hybrid vigour are extremely important in crop improvement. This system provides an unparalleled opportunity to shorten the breeding cycle and fix agronomic traits in the homozygous state. These androgenetic plants with Saccharum cytoplasm and Erianthus genome are male sterile making them highly useful for further hybridization and sugarcane improvement programme.

Improving complex agronomic and domestication traits in the perennial grain crop intermediate wheatgrass with genetic mapping and genomic prediction.

The perennial grass Thinopyrum intermedium (intermediate wheatgrass [IWG]) is being domesticated as a food crop. With a deep root system and high biomass, IWG can help reduce soil and water erosion and limit nutrient runoff. As a novel grain crop undergoing domestication, IWG lags in yield, seed size, and other agronomic traits compared to annual grains. Better characterization of trait variation and identification of genetic markers associated with loci controlling the traits could help in further improving this crop. The University of Minnesota's Cycle 5 IWG breeding population of 595 spaced plants was evaluated at two locations in 2021 and 2022 for agronomic traits plant height, grain yield, and spike weight, and domestication traits shatter resistance, free grain threshing, and seed size. Pairwise trait correlations were weak to moderate with the highest correlation observed between seed size and height (0.41). Broad-sense trait heritabilities were high (0.68-0.77) except for spike weight (0.49) and yield (0.44). Association mapping using 24,284 genome-wide single nucleotide polymorphism markers identified 30 main quantitative trait loci (QTLs) across all environments and 32 QTL-by-environment interactions (QTE) at each environment. The genomic prediction model significantly improved predictions when parents were used in the training set and significant QTLs and QTEs used as covariates. Seed size was the best predicted trait with model predictive ability (r) of 0.72; yield was predicted moderately well (r=0.45). We expect this discovery of significant genomic loci and mostly high trait predictions from genomic prediction models to help improve future IWG breeding populations.

N supply and yield of sugar beet after cover cropping: effects of cover crop species and management

Despite long-time practice, the effect of cover crop (CC) cultivation on N supply and yield of subsequent sugar beet (SB) is not clear for the temperate climate of Central Europe. To elucidate, four field trials with oil radish, spring vetch, saia oat, and winter rye CCs were conducted on typical loessial soil in Germany. Mineral fertilizer N dose was varied as second factor: zero (N0) and optimum dose (Nopt). Biomass characteristics before winter, soil mineral nitrogen content in spring (SMN), and sugar yield and total plant N content of SB were measured. The CC N effect (Neff), calculated as the difference in SB N uptake after CC minus bare fallow, was computed for the periods sowing-summer (July/August) and summer-harvest. On average, sugar yield was decreased after rye and increased after vetch CC compared to bare fallow which was more pronounced under N0 than Nopt. In the period sowing-summer, characterized by a high SB N demand, Neff was close to 0 kg N ha–1 after rye and up to +30 kg N ha–1 after vetch. In contrast, Neff in summer-harvest was negative, amounting up to –80 kg N ha–1 after radish and rye. Regression analysis revealed a significant relationship between Neff in the period sowing-summer and the sugar yield difference between the cover crop treatments and bare fallow (R2 = 0.29). A multiple regression analysis (R2 = 0.68) indicated that high CC biomass combined with a large negative spring SMN difference between CC treatment and fallow caused a large yield penalty, which can be compensated for by elevated N fertilization. Moreover, CC management ensuring either a limited CC biomass or low SMN difference of CCs compared to fallow by e.g., later sowing, less vital CC species or early CC termination, will likely secure higher SB yield after CCs than fallow.

Enhanced Efficiency Organo-Mineral Nitrogen Fertilizer improves Yield and Quality of Red Amaranth

Crop production with increased yield in a sustainable manner requires less environmental impact. Therefore, a pot experiment was accomplished to investigate the effect of newly developed enhanced efficiency nitrogen (N) fertilizer and conventional N fertilizers on the growth, yield, nutritional quality, and N use efficiency of red amaranth (Amaranthus tricolor cv. BARI lalshak 1). All the pots were placed at the experimental pot-house of the Department of Agricultural Chemistry, Bangladesh Agricultural University following a completely randomized design (CRD) with four replications. Brown coal-urea (BCU) was applied as enhanced efficiency N fertilizer following top dressing and basal application strategy where urea and diammonium phosphate were applied as conventional N fertilizer. All the N fertilizers were applied at five rates viz., 0, 50, 75, 100, 150 kg N ha ̶ 1. The growth parameters and biomass yield of red amaranth were significantly impacted by varying amounts and types of N fertilizers. The highest dry biomass yield was obtained at 150 kg N ha ̶ 1 whereas the highest number of branch plant ̶ 1 was found at 100 kg N ha ̶ 1. Again, the highest biomass N concentration was found at 100 kg N ha ̶ 1. Application of a higher dose of fertilizer showed the highest N concentration in the post-harvest soil. Overall, the growth parameters and biomass N concentration were the highest in BCU basal-treated soil. In contrast, BCU top-dressed soil showed a higher N concentration after harvest than all other treatments. Based on the overall findings, the use of higher-efficiency organo-mineral N fertilizer has the potential to serve as a viable substitute for traditional N fertilizers in the context of sustainable crop production with reduced environmental effects. J. of Sci. and Tech. Res. 5(1): 37-44, 2023

Role of SbNRT1.1B in cadmium accumulation is attributed to nitrate uptake and glutathione-dependent phytochelatins biosynthesis

Phytoremediation of cadmium (Cd)-polluted soil by using sweet sorghum displays a tremendous potential as it is a fast-growing, high biomass and Cd tolerant energy plant. Previous study has demonstrated SbNRT1.1B expression change is in accordance with enhanced Cd accumulation by external nitrate supply in sweet sorghum. Nevertheless, underlying mechanism of SbNRT1.1B response to Cd stress is still elusive. SbNRT1.1B exhibited a positive response to Cd stress in sweet sorghum. Overexpressing SbNRT1.1B increased primary root length, shoot fresh weight, nitrate and chlorophyll concentrations compared with Col-0 under Cd stress, while complementary SbNRT1.1B rescued these decreased values in mutant chl1–5. Cd concentrations in overexpressing SbNRT1.1B, complementary SbNRT1.1B and Col-0 lines were 3.2–4.1, 2.5–3.1 and 1.2–2.1 folds of that in chl1–5. Consistent with Cd concentrations, non-protein thiol (NPT), reduced glutathione (GSH) and phytochelatins (PCs) concentrations as well as the related genes expression levels showed the same trends under Cd stress. GSH biosynthesis inhibitor failed to reverse the patterns of GSH-dependent PCs concentrations changes in different lines, suggesting that SbNRT1.1B plays an upstream role in GSH-dependent PCs biosynthesis under Cd treatment. Altogether, SbNRT1.1B enhances nitrate concentrations contributing to increased chlorophyll concentrations and GSH-dependent PCs metabolites biosynthesis, thereby improving growth and Cd concentrations in plants.

Effect of biomass concentration in membrane bioreactor design on alpha factor for two large pilot systems: Case study of mechanical surface aeration and air insufflation system

In the literature, the studies on wastewater treatment process have shown a great interest in aeration system, most of them were performed on small pilot lab-scale. However, it still an important need to provide useful information aiming at reducing the operating costs and providing decision support to choose aeration devices. Considering that membrane bioreactors operating with high biomass concentrations up to 20–30 g of suspended solids (SS) per liter, the present study was carried out on a larger pilot lab-scale ( ± 500 l) of insufflation system and mechanical surface aeration on the same fresh sludge with concentration varying between 4.6 and 19.8 g/l. The comparison with literature data shows good correspondence with values obtained with our insufflation system but a great difference from values obtained in a mechanical system. In addition, this research provides a better insight on the calculation method that can be used to estimate the critical parameters of the oxygen transfer, and analyses the feasibility of air diffusion substitution by surface aeration system of Louvain La Neuve wastewater treatment plant of 13000 population equivalent. This extrapolation proved that a saving of ± 50,000 € per year can be achieved by choosing surface aeration rather than aeration by air insufflation.

Performance of length limits to sustain a data- and capacity-limited fishery

Length limit regulations are widely recommended to sustain fish biomass and fishery yields, particularly for data- and capacity-limited fisheries. However, previous findings were generally derived from equilibrium conditions, used hypothetical fisheries, and/or did not explicitly quantify the performance of length limits under a combination of implementation levels, increasing fishing effort, and discard mortality. Here, we apply the management strategy evaluation (MSE) framework to test the performance of three length-limit management procedures (MPs)—maturity and high maturity length limits (i.e., minimum length limits set at and above the length at maturity, respectively) and slot length limit (i.e., excludes both immature and very large fish from retention)—in Iran's largely unregulated, data- and capacity-limited kingfish fishery. We subject these MPs to different conditions, including strong and weak implementations, discard mortality, and an expansion in future fishing efforts, and quantify their overall performance in terms of fishing mortality, biomass, and yield. Our results show that strong implementation of length limits results in the largest gains: the lowest future long-term fishing mortality and the highest biomass and yields relative to the maximum sustainable yield (MSY) level. When illegal-sized fish are selected but discarded with high discard mortality under strong implementation, or retained under weak implementation, the benefits of length limits are severely reduced. Still, length limits are projected to result in much lower fishing mortality and maintain biomass and fishery yields near MSY levels compared to the current fishery's selectivity and retention. Over the entire projection period, the high maturity length limit provided the highest probability of sustainable biomass and the highest mean relative yield under weak implementation and a growing future fishing effort. Our results underscore the benefits of implementing length limit regulation in sustaining data- and capacity-limited fisheries, particularly if current fishing conditions involve selecting and retaining small and immature fish under a high and growing fishing mortality rate.

Allele-Specific Hormone Dynamics in Highly Transgressive F2 Biomass Segregants in Sugarcane (Saccharum spp.).

Sugarcane holds global promise as a biofuel feedstock, necessitating a deep understanding of factors that influence biomass yield. This study unravels the intricate dynamics of plant hormones that govern growth and development in sugarcane. Transcriptome analysis of F2 introgression hybrids, derived from the cross of Saccharum officinarum "LA Purple" and wild Saccharum robustum "MOL5829", was conducted, utilizing the recently sequenced allele-specific genome of "LA Purple" as a reference. A total of 8059 differentially expressed genes were categorized into gene models (21.5%), alleles (68%), paralogs (10%), and tandemly duplicated genes (0.14%). KEGG analysis highlighted enrichment in auxin (IAA), jasmonic acid (JA), and abscisic acid (ABA) pathways, revealing regulatory roles of hormone repressor gene families (Aux/IAA, PP2C, and JAZ). Signaling pathways indicated that downregulation of AUX/IAA and PP2C and upregulation of JAZ repressor genes in high biomass segregants act as key players in influencing downstream growth regulatory genes. Endogenous hormone levels revealed higher concentrations of IAA and ABA in high biomass, which contrasted with lower levels of JA. Weighted co-expression network analysis demonstrated strong connectivity between hormone-related key genes and cell wall structural genes in high biomass genotypes. Expression analysis confirmed the upregulation of genes involved in the synthesis of structural carbohydrates and the downregulation of inflorescence and senescence-related genes in high biomass, which suggested an extended vegetative growth phase. The study underscores the importance of cumulative gene expression, including gene models, dominant alleles, paralogs, and tandemly duplicated genes and activators and repressors of disparate hormone (IAA, JA, and ABA) signaling pathways are the points of hormone crosstalk in contrasting biomass F2 segregants and could be applied for engineering high biomass acquiring varieties.

Bycatch and discard rates assessment of the gillnet fishery in the southern Brazil

The fisheries discard patterns are an important element to be considered in stock assessment, fisheries management, and conservation measures. Using data collected by scientific observers on board, we analysed the bycatch and discard rates of the pelagic and bottom gillnet fishing in southern Brazil. Bottom gillnet was responsible for discarding from 77 taxa; 35 % of the discarded biomass was composed of teleost fishes, 33 % of elasmobranchs, and 22 % of other taxa. Pelagic gillnet, targeting the Pomatomus saltatrix, resulted in the discard of 13 species and taxa not identified at the species level, such as Cnidaria and Malacostraca. The most discarded fish species was Brevoortia pectinata, constituting 94.8 % of the total discarded biomass. The discard rate of bottom gillnets targeting Micropogonias furnieri was higher (20 %) than that of those targeting the demersal species (6 %), such as Cynoscion guatucupa (formely Cynoscion striatus) and Umbrina canosai. The high biomass proportion and numerous discarded species emphasize the need for effective management through temporal and spatial fishing restrictions. Our findings suggest potential measures, including the closure of fishing areas in coastal waters.

Investigating the Potential of Grass Biomass (Thysanolaena latifolia) as an Alternative Feedstock for Sugar Platforms and Bioethanol Production

Bioethanol, a lignocellulosic biofuel, has increased energy sustainability and lessened the environmental effects associated with energy production. Thysanolaena latifolia is a common weed found in the northern part of Thailand that is considered non-food biomass, with a high biomass productivity of approximately 10.2 kg/year. Here, we evaluated the potential of T. latifolia biomass as an environmentally friendly material source for producing alternative bioethanol. To this end, we treated the feedstock under mild conditions using various concentrations of phosphoric acid to create ideal conditions for enzymatic hydrolysis. Pretreatment with 75% phosphoric acid yielded the highest solid recovery (55.8 ± 0.6%) and glucans (93.0 ± 0.3%). Additionally, the hydrolysis efficiency and glucose yield of treated biomass were significantly improved. As a result, the liquid hydrolysate from T. latifolia used for ethanol fermentation by Saccharomyces cerevisiae TISTR 5339 generated 8.9 ± 0.0 g/L ethanol. These findings demonstrate that glucose derived from liquid hydrolysate is a promising sustainable carbon source for producing ethanol from T. latifolia feedstock. Thus, using T. latifolia as a feedstock for generating ethanol can improve the efficiency of bioenergy production.

Multi-stage oxic biofilm system for pilot-scale treatment of coking wastewater: Pollutants removal performance, biofilm properties and microbial community

A multi-stage oxic biofilm system based on hydrophilic polyurethane foam was established and operated for advanced treatment of coking wastewater, in which distinct gradient variations of pollutants removal, biofilm properties and microbial community in the 5 stages were evaluated. The system rapidly achieved NH4+-N removal efficiency of 97.51 ± 2.29 % within 8 days. The biofilm growing attached on the carriers exhibited high biomass (≥10.29 g/L), which ensured sufficient microbial population. Additionally, the rising extracellular polymeric substance and declining proteins/polysaccharides ratios across stages suggested a dense-to-loose transition in the biofilm’s structure, in response to the varying pollutant concentrations. The dominance of Nitrosomonas cluster in the first 3 stages and Nitrospira lineage in the following 2 stages facilitated the complete depletion of high NH4+-N concentration without NO2--N accumulation. Overall, the distinct biofilm property and community at each stage, shaped by the multi-stage configuration, maximized the pollutants removal efficiency.

Characteristics and assembly mechanisms of bacterial and fungal communities in soils from Chinese forests across different climatic zones

Forest soils are intricate ecosystems that harbor a diverse array of microorganisms, yet our current understanding of the characteristics and environmental implications of forest soil microbiomes remains limited. Here we present a continental-scale study on soil microbiomes of ten forests in China, spanning latitudes from 18°54′N to 50°48′N, resulting in a comprehensive catalog of bacterial and fungal operational taxonomic units (OTUs). Both bacterial and fungal communities exhibited discernible spatial variations in taxonomic and phylogenetic diversity. The composition of bacterial communities varied distinctively due to climatic zones—cold temperate, temperate, subtropical, and tropical—whereas fungal communities did not exhibit such pronounced distinctions. The co-occurrence network complexity of bacterial communities displayed a decremental trend along the cold temperate, temperate, subtropical, and tropical zones, whereas that of fungal communities displayed an incremental pattern. These results may be attributed to the facts that low temperatures sustain a high biomass of bacteria and foster increased interactions, while high temperatures and precipitation stimulate fungi-plant interactions. Furthermore, community assembly modeling revealed that forest soil microbial communities were dominated by stochastic processes. The bacterial community structure was mainly driven by homogeneous selection (26–41%) and dispersal limitation (35–44%), whereas dispersal limitation (51–56%) had the greatest impact on fungal community structure. Notably, bacterial functional genes associated with carbon fixation were more abundant in cold temperate soils compared to tropical soils. This study sheds light on spatial variations of forest soil microbiomes in China, enhancing further understanding of their response to global changes and implications for soil organic carbon cycles.

Botrytis cinerea causes different plant responses in grape (Vitis vinifera) berries during noble and grey rot: diverse metabolism versus simple defence.

The complexity of the interaction between the necrotrophic pathogen Botrytis cinerea and grape berries (Vitis vinifera spp.) can result in the formation of either the preferred noble rot (NR) or the loss-making grey rot (GR), depending on the prevailing climatic conditions. In this study, we focus on the functional gene set of V. vinifera by performing multidimensional scaling followed by differential expression and enrichment analyses. The aim of this study is to identify the differences in gene expression between grape berries in the phases of grey rot, noble rot, and developing rot (DR, in its early stages) phases. The grapevine transcriptome at the NR phase was found to exhibit significant differences from that at the DR and GR stages, which displayed strong similarities. Similarly, several plant defence-related pathways, including plant-pathogen interactions as hypersensitive plant responses were found to be enriched. The results of the analyses identified a potential plant stress response pathway (SGT1 activated hypersensitive response) that was found to be upregulated in the GR berry but downregulated in the NR berry. The study revealed a decrease in defence-related in V. vinifera genes during the NR stages, with a high degree of variability in functions, particularly in enriched pathways. This indicates that the plant is not actively defending itself against Botrytis cinerea, which is otherwise present on its surface with high biomass. This discrepancy underscores the notion that during the NR phase, the grapevine and the pathogenic fungi interact in a state of equilibrium. Conversely the initial stages of botrytis infection manifest as a virulent fungus-plant interaction, irrespective of whether the outcome is grey or noble rot.

Evaluation of three irrigation application systems for watermelon production in the Texas High Plains

AbstractAbout 90% of the Texas High Plains area depends on the water supply from the Ogallala aquifer. The decline of the Ogallala water table raises a concern for the sustainability of producing the traditional irrigated field crops due to their high water demand. Thus, diversifying cropping systems may be a solution. However, about 70% of the irrigated cropland uses center pivots that may negatively impact specialty crop quality. Thus, a 3‐year experiment was conducted to assess the potential of using mobile drip irrigation (MDI) to produce fresh watermelon compared to center pivot sprinkler irrigation using low‐elevation spray application (LESA), and surface drip irrigation with plastic mulch (DI). The study evaluated the effects of irrigation systems on crop yield, fruit quality, physiological responses, water use efficiency (WUE), and irrigation use efficiency (IUE). On average, plants under MDI had higher yields (106 Mg ha−1) than those under DI (70 Mg ha−1) and LESA (68 Mg ha−1). Plants under MDI and DI also had more fruits per plant (1.9) than those under LESA (1.4). The higher yield was associated with higher biomass and photosynthetic function. In general, fruit quality was not affected by irrigation system. In addition, plants under MDI system showed a higher WUE and IUE, and less water stress than those under LESA and DI systems. Results of this study suggest that irrigating under an MDI system is a good alternative for growers in the Texas High Plains that aim to diversify their cropping system with high‐value vegetable crops such as watermelon (Citrullus lanatus).