High Aeration Rate Research Articles

Effect of aeration rate and moisture content on the emissions of selected VOCs during municipal solid waste composting

The influence of the industrial control composting conditions (aeration 0.005–0.300 Lair kg−1 and moisture 40–70 %) of municipal solid waste on the composition of the selected compound emitted (limonene, β-pinene, 2-butanone, undecane, phenol, toluene, dimethyl sulfide, dimethyl disulfide) was studied. The highest emissions of volatile organic compounds (VOCs) were observed in the early stages of the processes. At the end of the process, low concentrations of the emitted compounds were found. Aeration rate had a strong effect on emissions. High aeration rate (0.300 Lair kg−1 min−1) caused normally high emissions of all selected compounds whereas low aeration rates (0.05 Lair kg−1 min−1) could cause anaerobiosis problems and generation of organic sulphur compounds. We observed that the effect of the moisture upon the emitted concentrations varied depending on the studied compound.

Scaling-up the production of thermostable lipolytic enzymes from Thermus aquaticus YT1

The lipolytic enzymes synthesized by Thermusaquaticus YT1 present extremely interesting properties of thermostability (more than 70% of activity after 12 days at 80°C and a half-life time of 1 h at 95°C), which point out the interest of proposing efficient strategies to successfully tackle the scale-up of the production process. In this study,viable scaling-up of the production process was implemented,and relevant aspects affecting the enzyme synthesis, such as the mineral composition of the culture medium, the aeration and the agitation have been evaluated.A strategy combining the modification of the culture medium and the aeration degree was also approached by adding perfluorocarbons, compounds which improve the availability of oxygen in the culture medium. An opposite response of biomass and lipolytic activity to the aeration conditions was found between scales (about 600 U L(-1) at high aeration levels in flask vs. 150 U L(-1) at high aeration rates in reactor), which further demonstrates the important role of the hydrodynamic conditions on the suitable development of the biological process. In all cases, the cultures were kinetically characterized and the Luedeking and Piret model turned out to be a valuable tool to conclude that the produced lipolytic enzyme is a growth-associated metabolite, no matter the medium and the scale.

Role of dissolved oxygen availability on lactobionic acid production from whey by Pseudomonas taetrolens

The influence of dissolved oxygen availability on cell growth and lactobionic acid production from whey by Pseudomonas taetrolens has been investigated for the first time. Results from pH-shift bioreactor cultivations have shown that high agitation rate schemes stimulated cell growth, increased pH-shift values and the oxygen uptake rate by cells, whereas lactobionic acid production was negatively affected. Conversely, higher aeration rates than 1.5Lpm neither stimulated cell growth nor lactobionic acid production (22% lower for an aeration rate of 2Lpm). Overall insights into bioprocess performance enabled the implementation of 350rpm as the optimal agitation strategy during cultivation, which increased lactobionic productivity 1.2-fold (0.58–0.7g/Lh) compared to that achieved at 1000rpm. Oxygen supply has been shown to be a key bioprocess parameter for enhanced overall efficiency of the system, representing essential information for the implementation of lactobionic acid production at a large scale.

Combined effects of agitation and aeration on the chitinolytic enzymes production by the Antarctic fungus Lecanicillium muscarium CCFEE 5003

BackgroundThe Antarctic fungus Lecanicillium muscarium CCFEE 5003 is one of the most powerful chitinolytic organisms. It can produce high level of chitinolytic enzymes in a wide range of temperatures (5-30°C). Chitinolytic enzymes have lot of applications but their industrial production is still rather limited and no cold-active enzymes are produced. In view of massive production of L. muscarium chitinolytic enzymes, its cultivation in bioreactors is mandatory. Microbial cultivation and/or their metabolite production in bioreactors are sometime not possible and must be verified and optimized for possible exploitation. Agitation and aeration are the most important parameters in order to allow process up-scaling to the industrial level.ResultsIn this study, submerged cultures of L. muscarium CCFEE 5003 were carried out in a 2-L bench-top CSTR bioreactor in order to optimise the production of chitinolytic enzymes. The effect of stirrer speed (range 200-500 rpm) and aeration rate (range 0.5-1.5 vvm) combination was studied, by Response Surface Methodology (RSM), in a medium containing 1.0% yeast nitrogen base and 1% colloidal chitin. Optimization was carried out, within a "quadratic D-optimal" model, using quantitative and quantitative-multilevel factors for aeration and agitation, respectively. The model showed very good correlation parameters (R2, 0.931; Q2, 0.869) and the maximum of activity (373.0 U/L) was predicted at ca. 327 rpm and 1.1 vvm. However, the experimental data showed that highest activity (383.7 ± 7.8 U/L) was recorded at 1 vvm and 300 rpm. Evident shear effect caused by stirrer speed and, partially, by high aeration rates were observed. Under optimized conditions in bioreactor the fungus was able to produce a higher number of chitinolytic enzymes than those released in shaken flasks. In addition, production was 23% higher.ConclusionsThis work demonstrated the attitude of L. muscarium CCFEE 5003 to grow in bench-top bioreactor; outlined the strong influence of aeration and agitation on its growth and enzyme production and identified the optimal conditions for possible production at the industrial level.

Influence of Aeration Rate on the Physio-Chemical Characteristics of Biodried Dairy Manure - Wheat Straw Mixture

Animal manure is a prime candidate for thermochemical conversion (co-firing, gasification, and pyrolysis) except for its high moisture content. Biodrying is a promising solution to reduce manure moisture with minimum energy input. This study investigated the effects of different aeration rates; low (0.05 L/min/kg VM), medium (0.80 L/min/kgVM), and high (1.50 L/min/kgVM), on the biodrying of dairy manure mixed with wheat straw. The moisture content dropped in 21 days from 56% to 28%, 34%, and 35% wb under high, medium, and low aeration rates, respectively. The heating value of the mixture decreased from 16.19 MJ/kgdry to 14.31, 13.79, and 13.83 MJ/kgdry under high, medium, and low aeration, respectively. Evaluation of energy consumption to remove a unit mass of moisture showed that high aeration levels required the least energy input, 7.84 MJ/kgH2O, followed by low aeration at 7.93 MJ/ kgH2O, while the medium aeration had a relatively high energy cost for water removal, 9.09 MJ/ kgH2O. The high aeration level was superior in terms of both drying energy and time requirements to the other rates considered. Comparison showed that high aeration level biodrying (1.50 L/min/kgVM) exceeded the range of conventional drying energy demand. However, the ratio of external fossil fuel demand was much lower in biodrying (due to the auto-thermal effect) than in conventional drying.

Influence of Control Parameters in VOCs Evolution during MSW Trimming Residues Composting

The influence of control parameters (aeration, moisture, and C/N ratio) during composting of a municipal solid waste (MSW)-legume-trimming residue (LTR) mixture was studied at a pilot plant scale. Factors measured included the composition of the main volatile organic carbons (VOCs) emitted including limonene, β-pinene, 2-butanone, undecane, phenol, toluene, and dimethyl disulfide. Polynomial models were found to reproduce the experimental results with errors at less than 10%. The relative influence of the independent variables on temperature and selected VOCs followed the order: aeration > moisture > C/N. A high aeration rate results in higher (strong negative effect) values on selected VOCs emissions (41-71% on emitted VOCs variation). Moisture had a positive and negative effect depending on the selected VOCs. A high C/N ratio caused lower production of VOCs except for undecane and 2-butanone. Providing an aerobic environment (0.05 Lair kg(-1) min(-1)), high C/N ratios (>50), and medium moisture (55%) minimize emitted VOCs during MSW composting, ultimately resulting in less odors in the surrounding environment.

Enhancement of survival rate of Pacific bluefin tuna (Thunnus orientalis) larvae by aeration control in rearing tank

High levels of larval mortality are a significant barrier to the artificial mass production of Pacific bluefin tuna (Thunnus orientalis ). Mortality may occur when larvae sink and come into contact with the bottom of the rearing tank during the first 10 days after hatching. We evaluated the effect of flow control by aeration on the survival of T. orientalis larvae. These larvae were held in 500-L tanks in which the aeration rate was varied during the night. Larval survival increased with air supply. We documented the cross-sectional flow pattern and gravitational sinking velocities of larvae to assess the correlation between survival and circulation patterns in the tank. The sinking velocity of T. orientalis larvae at night increased with larval body density, which varied with swimbladder volume. Larvae with uninflated swimbladders sank significantly faster than larvae with inflated swimbladders. Both water circulation speed and survival increased at higher aeration rates. Our results suggest that aeration rates >900 ml min–1 may increase larval survival by counteracting sinking.

Various operating conditions affecting the performance of aerobic digestion coupled with membrane filtration

An aerobic digestion coupled with membrane filtration (ADMF) system was investigated to determine the influence of various operating conditions to the efficiency of sludge thickening. Three different parameters were studied: the effect of hydraulic retention time (HRT), aeration and addition of coagulant. The effect of varying HRT was noticeable with the decrease in viscosity with increasing HRT. Transmembrane pressure, which indicates the extent of membrane fouling, increased with the decrease in HRT. Effect of aeration on the rate of increase in mixed liquor suspended solid concentration was observed to be insignificant, indicating that the aeration rate affected the filtration performance rather than the sludge reduction efficiency. Study on the effect of coagulant was evaluated by comparing two kinds of coagulants, organic and inorganic, at different concentrations. Dead-end filtration experiment and determination of critical flux were conducted to investigate the permeate performance according to coagulant addition. Higher value of critical flux was obtained with the addition of inorganic coagulant, while the total resistance was decreased with increasing coagulant concentration. Results show that while the addition of inorganic coagulant enhanced the ADMF system by improving membrane permeability, it is recommended that the system be operated at high HRT and aeration rate.

The Effect of Temperature and Aeration Rate on Bioremediation of Diesel-contaminated Soil in Solid-phase Bench-scale Bioreactors

Bioremediation of hydrocarbon (HC) contaminated soils is most effective in aerobic conditions. Despite the fact that mass transfer of oxygen is an important process parameter, the effect of this parameter on solid-phase bioremediation has received limited attention. In this study, the combined effect of temperature and aeration on the bioremediation of low organic content coarse-grained soils, freshly contaminated with diesel, was investigated in solid-phase bench-scale bioreactors. Total HC and carbon range soil concentrations, volatilization, and microbial activity were monitored throughout the six-month experiments at two temperatures (7 and 22°C) and at two aeration rates (13 and 45 mL·s−1). Total HC removal reached between 48 and 83%. Generally, removal increased proportionally with temperature and aeration rates, and decreased proportionally with HC compounds molecular weight. Both biodegradation and volatilization played important roles in removal in all treatments. The high aeration rate enhanced microbial activity in soil. Enhancement was believed to be due to increased mass transfer of oxygen from the soil gas to the soil solution, where microbial activity occurs. However, high aeration also enhanced volatilization, especially at 22°C where 51% of HCs were lost to volatilization. High aeration rate enhanced biodegradation of compounds > nC15 without promoting their excessive volatilization.

Identifying sources of nitrous oxide emission in anoxic/aerobic sequencing batch reactors (A/O SBRs) acclimated in different aeration rates

Both long term and batch experiments were carried out to identify the sources of the N 2O emission in anoxic/aerobic sequencing batch reactors (A/O SBRs) under different aeration rates. The obtained results showed that aeration rate has an important effect on the N 2O emission of A/O SBR and most of the N 2O was emitted during the aerobic phase. During the anoxic phase, nitrate ammonification was the major source of N 2O emission while denitrification performed as a sink of N 2O, in all three bioreactors. The N 2O emission mechanisms during the aerobic phase differed with the aeration rate. At low and high aeration rates (Run 1 and Run 3), both coupled-denitrification and nitrifier denitrification were ascribed to be the source of N 2O emission. At mild aeration rate (Run 2), nitrifier denitrification by Nitrosomonas-like ammonia oxidizing-bacterial (AOB) was responsible for N 2O emission while coupled-denitrification turned out to be a sink of N 2O because of the presence of inner anaerobic region in sludge flocs.

Influence of mist intervals and aeration rate on growth and second metabolite production of Pseudostellaria heterophylla adventitious roots in a siphon-mist bioreactor

Plant adventitious root culture in bioreactors is a promising alternative for the efficient production of medicinal herbs. Adventitious roots of Pseudostellaria heterophylla were induced from callus and then cultivated in a siphon-mist bioreactor. An orthogonal test established that the optimal medium for adventitious root induction was MS medium supplemented with 1.0 mg/L naphthaleneacetic acid and 2.0 mg/L 3-indolybutyric acid. Under these conditions, the average root number was more than 14 on each 1.0 cm diameter callus and the rooting rate reached 100%. The bioreactor was equipped with an integral siphon-spraying device designed to automatically supply the liquid medium. The operation parameters of the bioreactor were assessed by varying the mist interval and the aeration velocity. The mist interval was negatively related to average growth rate of the adventitious roots and positively related to saponin and polysaccharide content. A relatively high aeration rate was necessary to achieve the maximum biomass production, but the secondary metabolite production was not enhanced by increasing the aeration velocity.

Effect of aeration and agitation on synthesis of poly (γ-glutamic acid) in batch cultures of Bacillus licheniformis NCIM 2324

The effects of aeration and agitation on the production and molecular weight of poly (γ-glutamic acid) (PGA) were systematically investigated in batch fermentor cultures of Bacillus licheniformis NCIM 2324. A high aeration rate and agitation speed enhanced the growth of B. licheniformis NCIM 2324, but did not always lead to high PGA production. Additionally, PGA production actually decreased at very high aeration rates and agitation speeds. The maximum PGA concentration was obtained at 750 rpm and 1 vvm. Rheological studies revealed that fermentation broth during production of PGA exhibited pseudoplastic behavior. The effects of aeration and agitation on the molecular weight of PGA were also studied, and the rate and extent of the decrease in the molecular weight of PGA as a function of time were found to be much greater at high aeration than low aeration. The PGA production of 46.34 g/L with a specific productivity of 0.17 g-PGA/g-biomass/ h and a PGA yield of 0.48 with respect to total substrate observed in the present study are much higher than the values reported in previously conducted studies.

Effect of cycloheximide, iodoacetamide, and antimycin A on inorganic phosphate synthesis in Saccharomyces cerevisiae VKM Y-1173

The effect of inhibitors of protein synthesis (cycloheximide, CHI), glycolysis (iodoacetamide, IAA), and oxidative phosphorylation (antimycin A, ANM) on inorganic phosphate (polyP) synthesis during the first 0.5 h of their hypercompensation in Saccharomyces cerevisiae VKM Y-l173 grown on 2% glucose-containing media at low (hypoxia) or high aeration rates or in the presence of 1 vol % ethanol under high aeration conditions was studied. PolyP accumulation was highest in the medium with glucose under hypoxia; lower, with glucose at high aeration; and lowest, in the medium with ethanol. CHI had a small effect on the total polyP level but significantly stimulated ATP accumulation, irrespective of the culture growth conditions. The low-polymer acid-soluble polyP1 were synthesized most actively by the cells grown on glucose under hypoxia, alkali-soluble polyP3 were synthesized at en hanced aeration, and the most hig-molecular fraction, polyP5, was actively accumulated along with polyP3 at cultivation on ethanol. Regardless of the growth conditions, CHI inhibited accumulation of polyP4, the synthesis of which is associated with the synthesis of mannoproteins. IAA and ANM largely inhibited synthesis of all fractions at yeast growth under hypoxia and on ethanol, respectively. The results as a whole demonstrate the dependence of polyP formation on the main energy-generating cell processes and, at the same time, the absence of direct dependence of their synthesis on ATP concentration in Saccharomyces cerevisiae VKM Y-l 173.

High aeration rate enhances flow stratification in full-scale oxidation ditch

Aerated channel reactors with a uniform field of aeration may display flow stratification and short-circuit phenomena in wastewater treatment systems. In this study, we present data suggesting that flow stratification is closely related to the aeration rate and the arrangement of aerators. A full-scale oxidation ditch, with a total volume of 6,500 m(3) and a membrane-diffused aerated zone of 60 x 7 x 5 m (length-width-depth), was selected for water velocity measurements. Two profiles of the oxidation ditch were studied in detail: the first one was at the end of the aerated zone and the second one at the end of the anoxic zone. The results of this work demonstrate that the horizontal water velocity at the end of the aerated zone displayed significant stratification, with maximum velocity near the water surface (0.5-0.7 m/s) and almost zero velocity at a depth of 2.5 m. At the end of the anoxic zone, water velocity was uniform and equal to 0.27-0.31 m/s. Increasing the aeration rate from 1,800 to 4,300 m(3)/h, almost 90% of the water flow was found to discharge through the upper-half of the channel reactor profile. Different options to mitigate flow stratification of the oxidation ditch are discussed in this paper.

Coupling sequencing batch airlift reactor (SBAR) and membrane filtration: Influence of nitrate removal on sludge characteristics, effluent quality and filterability

This study investigates the sludge and effluent characteristics of a new process of coupling an aerobic granular sludge bioreactor with a membrane filtration. The effluent and mixed liquor of sequencing batch airlift reactor (SBAR) were analyzed at various aeration shear stresses when fed with high nitrate containing wastewater. The presence of nitrate nitrogen and aerobic/anoxic condition was able to improve the sludge characteristics in terms of biomass retention, density and settling ability in SBAR. MLSS and SVI could reach 9 g/L and 44 mL/g respectively at the aeration rate of 0.6 cm/s. The presence of nitrate and the denitrification process could minimize the fouling potential. The membrane fouling can be better correlated to SBAR sludge characteristics than biomass concentration. The high aeration rate in the reactor increased the fouling resistance due to production of large MW soluble microbial products (30–50 kDa). The soluble fraction of SBAR effluent contained mainly hydrophilic substances when nitrate is present in the wastewater.

RETRACTED: Influence of aeration rate on nitrogen during blackwater composting

The paper aimed to study the influence of the aeration rate on nitrogen dynamics during the composting of Blackwater sludge with sawdust. The increased aeration was responsible for an increase in ammonia emissions and for the decrease in nitrogen losses through leaching. At high aeration rates, losses of nitrogen in leachates and as ammonia in exhaust gas accounted for 90.99% of the nitrogen removed from the material. At low aeration rates, those accounted for 47.85% of the nitrogen removed from the material. The nitrification rate was found to be proportional to the mean content of NH 4 + /NH 3 in the material, i.e., initial NH 4 + /NH 3 plus NH 4 + /NH 3 released by ammonification minus the losses in leachates and in exhaust gas. The aeration rate was shown to be a main parameter affecting nitrogen dynamics during composting, since it controlled the ammonification, the ammonia emission and the nitrification processes.

Alternating anoxic feast/aerobic famine condition for improving granular sludge formation in sequencing batch airlift reactor at reduced aeration rate

In this study the influence of a pre-anoxic feast period on granular sludge formation in a sequencing batch airlift reactor is evaluated. Whereas a purely aerobic SBR was operated as a reference (reactor R2), another reactor (R1) was run with a reduced aeration rate and an alternating anoxic-aerobic cycle reinforced by nitrate feeding. The presence of pre-anoxic phase clearly improved the densification of aggregates and allowed granular sludge formation at reduced air flow rate (superficial air velocity (SAV) = 0.63 cm s −1). A low sludge volume index (SVI 30 = 45 mL g −1) and a high MLSS concentration (9–10 g L −1) were obtained in the anoxic/aerobic system compared to more conventional results for the aerobic reactor. A granular sludge was observed in the anoxic/aerobic system whilst only flocs were observed in the aerobic reference even when operated at a high aeration rate (SAV = 2.83 cm s −1). Nitrification was maintained efficiently in the anoxic/aerobic system even when organic loading rate (OLR) was increased up to 2.8 kg COD m −3 d −1. In the contrary nitrification was unstable in the aerobic system and dropped at high OLR due to competition between autotrophic and heterotrophic growth. The presence of a pre-anoxic period positively affected granulation process via different mechanisms: enhancing heterotrophic growth/storage deeper in the internal anoxic layer of granule, reducing the competition between autotrophic and heterotrophic growth. These processes help to develop dense granular sludge at a moderate aeration rate. This tends to confirm that oxygen transfer is the most limiting factor for granulation at reduced aeration. Hence the use of an alternative electron acceptor (nitrate or nitrite) should be encouraged during feast period for reducing energy demand of the granular sludge process.

Effect of practical diets with different protein levels on the performance ofFarfantepenaeus paulensisjuveniles nursed in a zero exchange suspended microbial flocs intensive system

Farfantepenaeus paulensis juveniles (72 ± 24 mg), were reared in a suspended microbial flocs system and fed practical diets containing increasing amounts of crude protein (250, 300, 350, 400 and 450 g kg−1 CP). Development of microbial flocs was promoted by high aeration rates and fertilization with wheat bran and molasses. Flocs were composed of detritus in the form of flocculated matter colonized by heterotrophic bacteria, cocoid and filamentous cyanobacteria, flagellate and ciliate protozoa and rotifers. Proximate composition analysis of the suspended microbial floc showed CP levels of 304 g kg−1. After 45 days, mean shrimp survival were above 89%, with no significant differences between treatments. Shrimp fed diets with 350 g kg−1 or higher CP content achieved significant higher (P < 0.05) final weight (0.66–0.68 g), weight gain (0.58–0.61 g) and instantaneous growth rate (0.049–0.050), with feed conversion rates (2.17–2.30) significantly lower (P < 0.05). Results show that, when rearing is carried out in a suspended microbial flocs system, dietary CP levels can be kept at 350 g kg−1. Furthermore, results confirm that microbial-based systems allow shrimp culture without compromising the surrounding environment and shows the possible reduction of production costs and fish meal dependence.

Kinetics and oxidative mechanism for H 2O 2-enhanced iron-mediated aeration (IMA) treatment of recalcitrant organic compounds in mature landfill leachate

A hydrogen peroxide (H 2O 2)-enhanced iron (Fe 0)-mediated aeration (IMA) process has been recently demonstrated to effectively remove organic wastes from mature landfill leachate. In this paper, the kinetics and oxidative mechanisms of the enhanced IMA treatment were studied. Bench-scale full factorial tests were conducted in an orbital shaker reactor for treatment of a mature leachate with an initial chemical oxygen demand (COD) of 900–1200 mg/L. At the maximum aeration rate (8.3 mL air/min mL sample), process variables significantly influencing the rates of H 2O 2 decay and COD removal were pH (3.0–8.0), initial H 2O 2 doses (0.21–0.84 M), and Fe 0 surface area concentrations (0.06–0.30 m 2/L). Empirical kinetic models were developed and verified for the degradation of H 2O 2 and COD. High DO maintained by a high aeration rate slowed the H 2O 2 self-decomposition, accelerated Fe 0 consumption, and enhanced the COD removal. In hydroxyl radical (OH ) scavenging tests, the rate of removal of glyoxylic acid (target compound) was not inhibited by the addition of para-chlorobenzoic acid (OH scavenger) at pH 7.0–7.5, ruling out hydroxyl radical as the principal oxidant in neutral–weakly basic solution. These experimental results show that this enhanced IMA technology is a potential alternative for the treatment of high strength recalcitrant organic wastewaters.

Production of a potentially novel antimicrobial compound by a biofilm-forming marine Streptomyces sp. in a niche-mimic rotating disk bioreactor

After initial small-scale experiments, a 25.0 l rotating disk bioreactor (RDBR) was investigated for the cultivation of a biofilm-forming salt-tolerant Streptomyces sp. MS1/7, producing an antimicrobial compound. Peak activity attainment rate, PAAR (ratio of the peak antimicrobial activity, PAMA and the time taken to attain PAMA) was determined. Of the three pH values examined (8.0, 9.0 and 10.0) maximum PAAR (1.82 mm/h) was attained at pH 9.0. Three aeration rates (9.0, 6.0 and 3.0 l/min) were considered at three levels (25, 50 and 75%) of disk submergence. At the highest aeration rate and 50% submergence level, PAMA (41 mm), PAAR (1.86 mm/h) and biofilm density (BD, 0.91 g/ml) attained their highest values. At any given aeration rate, PAMA was always higher at 50% submergence level. This supports our earlier premise that ideal intertidal conditions, 12 h periods of immersion and emersion, promote maximum BD and antimicrobial production in the niche-mimic RDBR.