High Yields Of Fermentable Sugars Research Articles

Efficient and accurate fractionation of eucalyptus using glycolic acid-catalyzed hydrothermal treatment and alkali treatment comprehensive technology

The effective utilization of lignocellulosic biomass is the key to evaluating the profitability of the process, and precise fractionation is a prerequisite for effective utilization. In this study, a high yield of fermentable sugars and lignin rich in phenolic hydroxyl groups were successfully obtained from eucalyptus using glycolic acid-catalyzed hydrothermal and alkaline treatment. The results showed that 88.20% of xylan and 85.07% of lignin were separated, and 62.68% of lignin was extracted from the hydrolysate. The extracted lignin has a complete structure and is rich in guaiacyl and syringyl units, which is conducive to the subsequent high-value utilization of lignin. 85% of glucan was recovered, and the glucose conversion yield was 84.24% after enzymatic hydrolysis. The hydrolysate can be concentrated, impurity removed, and purified to produce biofuels or chemicals after the precipitation of lignin. In summary, the two-step process based on glycolic acid has great potential in converting eucalyptus into biomass energy and chemicals, and this process is a promising precision fractionation strategy.

Experimental and kinetic analyses of delignification of lignocellulosic grass with minimal holocellulose loss during pretreatment

This work aims to develop an experimental and theoretical framework for converting the holocellulose present in an agro-residue to bioethanol without separating the cellulose and the hemicellulose from each other. Three delignification methods are employed to reduce holocellulose loss, and achieve a high yield of fermentable sugars through enzymatic hydrolysis: sodium chlorite-acetic acid (SC-AA), sodium chlorite-acetone (SC-AC), and separate SC-AA. The experimental methodology and theoretical analyses suggest that the separate SC-AA method is an effective delignification technology that preserves the maximum possible holocellulose content (11.7 % loss) while simultaneously removing lignin from agro-residues. Furthermore, this process also increases the accessibility of the holocellulose pores by restricting the adsorption of delignified products in the pores of the substrate, thus increasing the pore volume available for enzymatic hydrolysis reactions. The multi-stage modeling approach predicts the reaction time for delignifying agro-residues and quantifies the impact of reaction parameters on holocellulose retention.

Optimization of Concentrated Sulphuric Acid Hydrolysis of Gadam Sorghum Stalks Found in Kenya for Fermentable Sugar Production

Gadam sorghum stalks are agricultural residues which can be hydrolyzed into fermentable sugars that can be used to produce bioethanol which is a renewable source of energy. In order to produce bioethanol from lignocellulosic biomass such as Gadam sorghum stalks, several processes including hydrolysis are involved. However, the use of lignocellulosic biomass for bioethanol production is hindered by the low yield of fermentable sugars obtained during hydrolysis. The lack of sufficient information on optimal conditions governing hydrolysis of lignocellulosic biomass leads to inefficient process which hinders the economic viability of large-scale bioethanol production. The objective of this study was to optimize reaction conditions involved in concentrated sulphuric acid hydrolysis of Gadam sorghum stalks. During hydrolysis, the conditions that were varied included temperature (40°C–80°C), time (30-90 minutes), and concentration of acid (30%-70%,w/w). Central composite rotatable design was used to optimize and establish optimum level of hydrolysis conditions. Response surface methodology and analysis of variance were used to interprete the results. The results of hydrolysis revealed that the highest yield of glucose was 87.54% (w/w) which was realized at 60°C hydrolysis temperature, 60 minutes hydrolysis period, and 50% (w/w) concentration of sulphuric acid. In addition, the lowest glucose yield was 45.59% (w/w) which was realized at 60°C hydrolysis temperature, 60 minutes hydrolysis period, and 16.36% (w/w) sulphuric acid concentration. Concentrated sulphuric acid hydrolysis of Gadam sorghum stalks results in high yield of fermentable sugars. These results reveal that Gadam sorghum stalks are viable substrates for the production of fermentable sugars.

Statistical optimization of pretreatment of municipal solid waste by response surface methodology for fermentable sugar production

AbstractThis research aims to use low‐cost and easily available chemicals for chemical pretreatment to achieve a high yield of fermentable sugars using biodegradable municipal solid waste as a substrate. Biodegradable municipal solid waste was pretreated with locally available toilet cleaner (acidic in nature) and washing detergent (basic in nature) and to achieve maximum yield of reducing sugars, two variables; chemical concentration and time were selected for optimization using a central composite design. In Central composite design 13 runs for toilet cleaner (acidic in nature) and washing detergent (basic in nature) pretreatment models were created while ranges for two variables were selected as 1, 2, 3 days for time and 1%, 3%, 5% for chemical concentration. In the toilet cleaner (acidic in nature) pretreatment, optimum conditions were 5% (vol/vol) of toilet cleaner (acidic in nature) for 3 days. At optimal conditions, the model for toilet cleaner (acidic in nature) pretreatment released 167.755 g/L of reducing sugars and 2.684 MJ/L energy while the model for washing detergent (basic in nature) pretreatment released 159.141 g/L of reducing sugars and 2.546 MJ/L energy. An enzyme cocktail hydrolyzed the toilet cleaner (acidic in nature) pretreated sample, and the highest value of fermentable sugar was achieved at 36 h of 237.83 ± 11.028 g/L. Thus, the chemical pretreatment using toilet cleaner and washing detergent has a great potential for fermentable sugar production and effectively be used in industrial and household digesters for the conversion of waste into useful products.

Highly-efficient pretreatment using alkaline enhanced aqueous deep eutectic solvent to unlock poplar for high yield of fermentable sugars: Synergistic removal of lignin and mannan

Herein, a short-time alkaline enhanced aqueous DES (AaDES) pretreatment using choline chloride/ethylene glycol was reported, aiming at enhancing cellulose and xylan enzymatic digestibility. Simultaneously, saccharification efficiency of cellulose and xylan was reached to 91.2% and 99.0%, respectively, ∼4 and ∼ 24 times that of raw poplar. Pretreatment time was substantially shortened from 15-24 h to 4 h. Notably, 43.00 kg fermentable sugars (73% of the theoretical maximum) and 12.98 kg lignin with rich β-O-4′ linkages were obtained based on 100 kg poplar. The complete removal of acetyl and partial removal of lignin and mannan contributed to excellent pretreatment performance. It was found that enzymatic digestibility of xylan/cellulose was positively correlated with removal of mannan (R2 = 0.9719; R2 = 0.9010) and delignification (R2 = 0.6888; R2 = 0.8293). Drastic reduction in pretreatment time along with high-yield sugars in AaDES system will provide strength towards industrial level biorefinery.

Lowering energy consumption for fermentable sugar production from Ramulus mori: Engineered xylanase synergy and improved pretreatment strategy

Biorefinery of Ramulus mori with lower energy consumption through improved enzyme and pretreatment strategies was reported. Directed evolution and saturation mutagenesis were used for the modification of xylanase, the yield of fermentable sugars and the degree of synergy (DS) were determined for different pretreatment (seawater/non-seawater) and enzyme treatment groups (xylanase/cellulase/co-treatment). The dominant mutant I133A/Q143Y of Bispora sp. xylanase XYL10C_ΔN was obtained with improved specific activity (1860 U/mg), catalytic efficiency (1150 mL/s∙mg) at 40 °C, and thermostability (T50 increased by 7 °C). With the pretreatment of seawater immersion, the highest yield of fermentable sugars for Ramulus mori at 40 °C reached 199 μmol/g when hydrolyzed with cellulase and I133A/Q143Y, with the highest DS of 2.6; this was 4.5-fold that of the group hydrolyzed by cellulase alone with non-seawater pretreatment. Thus, bioconversion of reducing sugar from Ramulus mori was improved significantly at lower temperatures, which provides an efficient and energy-saving wayfor biofuel production.

Loop engineering of a thermostable GH10 xylanase to improve low-temperature catalytic performance for better synergistic biomass-degrading abilities

Lignocellulosic biorefining for producing biofuels poses technical challenges. It is usually conducted over a long time using heat, making it energy intensive. In this study, we lowered the energy consumption of this process through an optimized enzyme and pretreatment strategy. First, the dominant mutant M137E/N269G of Bispora sp. MEY-1XYL10C_ΔN was obtained by directed evolution with highcatalytic efficiency (970 mL/s∙mg)and specific activity (2090 U/mg)at 37 °C, and thermostability was improved (T50 increased by5 °C). After pretreatment with seawater immersionfollowing steam explosion,bagasse was co-treated with cellulase and M137E/N269G under mild conditions (37 °C), the resulting highest yield of fermentable sugars reached 219 µmol/g of bagasse,46% higher than that of the non-seawater treatment group, with the highest degree of synergy of 2.0. Pretreatment with seawater following steam explosion and synergistic hydrolysis through high activity xylanase and cellulase helped to achieve low energy degradation of lignocellulosic biomass.

Optimization of sugar production from Durian seeds via alkaline hydrolysis for second-generation bioethanol production

Abstract As one way to eliminate the issues found in the preceding generation, feedstock exploration in second-generation bioethanol production remains an issue, especially for a tropical country such as Indonesia. From exotic fruit by-products, durian holds a promising perspective that rests on its abundance, superb carbohydrate content and limited usage until now. This work presents the first-ever utilization of durian seeds for sugar production under optimized conditions through alkaline hydrolysis. A simple form of sugar was extracted by varying four parameters, namely substrate loading, NaOH concentration, hydrolysis time and hydrolysis temperature. Response surface methodology based on the Box-Behnken design was employed to outline the most optimum parameter values. Analysis of variance revealed that the quadratic model fit the data appropriately with the order of significance as substrate loading > hydrolysis time > NaOH concentration > hydrolysis temperature. The optimized conditions for reducing sugar yield, as high as 2.140 g/L, corresponded to <50 g/L substrate loading, 0.522 M NaOH, 60 minutes of hydrolysis time and 80oC hydrolysis temperature. The possible ethanol content of 1.094 g/L was also expected under optimized conditions, demonstrating great potential in second-generation bioethanol production. Second-generation bioethanol production from a non-edible feedstock (durian seeds) is optimized by varying key parameters in the alkaline hydrolysis process, showing high yields of fermentable sugars.

Optimization of peroxide-alkaline pretreatment and enzymatic hydrolysis of barley straw (Hordeum vulgare L.) to produce fermentable sugars using a Box–Behnken design

In recent years, interest has increased in the use of agro-industrial wastes for use as raw materials in the production of second-generation biofuels and other products of industrial interest. A pretreatment is necessary, followed by an enzymatic hydrolysis to obtain high yields of fermentable sugars. In the present study, the best conditions were established both for peroxide-alkaline pretreatment and for enzymatic hydrolysis of the Mexican barley straw (Hordeum vulgare L.) variety Dona Josefa in order to maximize lignin removal and improve enzyme conversion. Response surface methodology based on a Box–Behnken experimental design was used to optimize pretreatment conditions (concentration of hydrogen peroxide [H2O2], reaction time, and liquid–solid ratio (LSR)), and enzymatic hydrolysis (cellulase and xylanase concentrations and reaction time). Samples obtained from residual bagasse at each process stage were analyzed by means of scanning electron microscopy (SEM) and infrared spectrometry (FTIR) to evaluate the changes in the structure of the material after treatments. In pretreatment optimization, it was possible to obtain 60% delignification at a 6% peroxide concentration and a 12:1 LSR in 24 h. In saccharification, a maximum 82% conversion of cellulose to glucose using 6% cellulase (Cellic® CTec III), and 4% xylanase (Cellic® HTec III) in 20 h of hydrolysis, was used in obtaining a maximum sugar concentration of 70 g/L. Peroxide-alkaline pretreatment together with state-of-the-art enzyme saccharification makes barley straw an excellent alternative for fermentable sugar production that can be used for various biotech industries.

Process optimisation for saccharification and fermentation of wheat straw for the production of single cell protein

Present study demonstrates the utilisation of wheat straw for the production of SCP. Different concentrations of sulphuric acid were used at different temperatures to obtain high yield of fermentable sugars from wheat straw. Response surface methodology (RSM) was employed to optimise the different variables for the enzymatic saccharification of the hydrolysates produced using acid saccharification. The isolated fermentable sugars were used as fermentation media for production of SCP using saccharomyces cerevisiae and the process of fermentation was optimised using different starter culture concentrations and incubation times. Among the acid saccharification, 0.75% sulphuric acid at 121°C for 30 min resulted in highest amount of fermentable sugars whereas; among the enzymatic saccharification, 3.78% crude enzyme, 4.59 pH, 45°C temperature and 6.93 hours incubation time resulted in highest yield of fermentable sugars. Highest concentration of biomass (SCP) 22.3 ×107 cells/ml was observed using 2.5% starter culture concentration for 20 hours incubation.

Screening and evaluation of cellulytic fungal strains for saccharification and bioethanol production from rice residue

In this study, microwave-assisted alkali and acid pretreated rice straw were used to improve fermentable sugar yield by enzymatic saccharification (ES) employing cellulolytic fungal strains and subsequent bioethanol production by using fermenting yeast. The cellulolytic fungal strains Trichoderma reesei NCIM 1052, 1186, 992, T. reesei ITCC 4025, 6413, Aspergillus niger ITCC 302, A. acculeatus ITCC 5078, A. fumigates ITCC 4768 and Fusarium Solani ITCC 6397 were used for enzymatic saccharification (ES) of acid/alkali pretreated rice straw to optimize the sugar recovery. T. reesei NCIM 1052 was found superior as compared to the other fungal strains in terms of FPase, CMCase activities, and reducing sugars yield from pretreated rice straw. The strains of Saccharomyces cerevisiae NCIM 3186, and Pichia stipitis NCIM 3499 were used for subsequent fermentation to produce bioethanol. The saccharification of alkali pretreated rice straw (2% v/w NaOH) by T. reesei NCIM 1052 resulted in the highest fermentable sugar yield (55.6 g/l) and ultimately, the higher ethanol concentration after 72 h of fermentation with P. stipitis NCIM 3499 (25.3 g/L) as compared to other yeast strains. This study also exhibits the high potential for economic generation of ethanol from rice straw.

Alkali delignification and Bacillus sp. BMP01 hydrolysis of rice straw for enhancing biofuel yields

BackgroundLignocellulose biomass such as agricultural residues is increasingly important for biofuel production. Using agricultural residues like rice straw for biofuel production has dual purposes of utilizing the huge waste generated. The abundant availability and high polysaccharide contents are the main reason for biofuel production. However, the use is limited due to the reluctant nature of lignin in the biomass. Therefore, sodium hydroxide was used for solubilizing lignin and preserving the polysaccharides.ResultsAbout 71.29% of lignin (maximum amount of lignin) was removed after 7% sodium hydroxide (NaOH) pretreatment followed by 3% pretreatment (about 67.13%). Also, maximum cellulose (about 71.33%) was preserved after 7% NaOH pretreatment. Results of reducing sugars obtained by spectrophotometric analysis (3,5-dinitrosalycilic acid method) of pretreated rice straw show about 88.27% conversion (753 mg/g) after the 13th day of hydrolysis by bacteria isolated from a termite. FE-SEM, XRD, FTIR, and TGA analyses show a significant amount of lignin was removed which helps in releasing cellulose tangled in lignin.ConclusionRice straw pretreatment at 7% NaOH and hydrolysis by Bacillus sp. BMP01 achieved the release of a high yield of fermentable sugars that increased the yield of bioethanol. This study demonstrates the potential of alkali pretreatment coupled with microbial hydrolysis for efficient bioethanol production from agricultural residues like rice straw.

Co-production of xylooligosaccharides and fermentable sugars from poplar through acetic acid pretreatment followed by poly (ethylene glycol) ether assisted alkali treatment

A novel combined pretreatment process of poplar sawdust was established in this study based on the sequential acetic acid and alkali treatment with poly (ethylene glycol) diglycidyl ether (PEGDE). Effects of each treatment step on chemical composition, cellulose accessibility, and enzymatic digestibility of poplar sawdust was investigated. Acetic acid pretreatment remarkably increased cellulose accessibility while also producing a relatively high quantity of xylooligosaccharides (XOS) (37.6% of raw xylan). However, enzymatic digestibility remained low (28.3%) despite hemicellulose disruption. Post alkali treatment was next applied, leading to improvement on cellulose accessibility and enzymatic hydrolysis. Enzymatic hydrolysis was improved more significantly by successive alkali treatment with PEGDE. Its potential mechanisms attributable to enzymatic hydrolysis improvement were explored by revealing the changes to lignin properties. This work successfully demonstrated that recalcitrant waste woody biomass can be biorefined into both high-value XOS as well as relatively high yield of fermentable sugars.

A novel β-xylosidase from Anoxybacillus sp. 3M towards an improved agro-industrial residues saccharification

An intracellular β-xylosidase (AbXyl), from the thermoalkaline Anoxybacillus sp. 3M, was purified and characterized. The homodimeric enzyme (140 kDa) was optimally active at 65 °C and pH 5.5, exhibited half life of 10 h at 60 °C, 78 and 88% residual activity after 24 h, at pH 4.5 and 8.0, respectively. Fe2+, Cu2+, Al3+, Ag+ and Hg2+ inhibited the enzyme; the activity was moderately stimulated by SDS and not influenced by β-mercaptoethanol. In the presence of p-nitrophenyl-β-d-xylopyranoside, AbXyl exhibited Km of 0.19 mM, Kcat of 453.29 s−1, Kcat Km−1 of 2322 s−1 mM and was moderately influenced by xylose (Ki 21.25 mM). The enzyme hydrolyzed xylo-oligomers into xylose and catalyzed transxylosilation reactions also in presence of alcohols as acceptors, producing xylo-oligosaccharides and alkyl-xylosides. Finally AbXyl was applied towards a statistically optimized process of brewery's spent grain bioconversion, highlighting the important role of this biocatalyst in reaching high yields of fermentable sugars.

Subcritical water hydrolysis of sugar beet pulp towards production of monosaccharide fraction

Sugar beet pulp represents one of the most abundant by-product in food industry in terms of underexploited opportunities and amounts produced. In order to produce added-value product from this lignocellulosic material, subcritical water hydrolysis was applied in this study. Response surface methodology was used to evaluate the effects of hydrolysis parameters and optimize conditions for various responses. Hydrolysis temperature, hydrolysis time and HCl concentration were investigated as independent variables. The results obtained indicated highly promising application of corresponding hydrolysis technique towards valorization of proposed raw material. Relatively high yield of fermentable sugars with the addition of acid agent and the higher hydrolysis rates of lignocellulosic material compared to the previously published studies were obtained. Hydrolysis efficiency was higher than 50% and total monosaccharide yield reached up to 5.669 mg/mL which is significantly higher than previously reported results.

Gene stacking of multiple traits for high yield of fermentable sugars in plant biomass

BackgroundSecond-generation biofuels produced from biomass can help to decrease dependency on fossil fuels, bringing about many economic and environmental benefits. To make biomass more suitable for biorefinery use, we need a better understanding of plant cell wall biosynthesis. Increasing the ratio of C6 to C5 sugars in the cell wall and decreasing the lignin content are two important targets in engineering of plants that are more suitable for downstream processing for second-generation biofuel production.ResultsWe have studied the basic mechanisms of cell wall biosynthesis and identified genes involved in biosynthesis of pectic galactan, including the GALS1 galactan synthase and the UDP-galactose/UDP-rhamnose transporter URGT1. We have engineered plants with a more suitable biomass composition by applying these findings, in conjunction with synthetic biology and gene stacking tools. Plants were engineered to have up to fourfold more pectic galactan in stems by overexpressing GALS1, URGT1, and UGE2, a UDP-glucose epimerase. Furthermore, the increased galactan trait was engineered into plants that were already engineered to have low xylan content by restricting xylan biosynthesis to vessels where this polysaccharide is essential. Finally, the high galactan and low xylan traits were stacked with the low lignin trait obtained by expressing the QsuB gene encoding dehydroshikimate dehydratase in lignifying cells.ConclusionThe results show that approaches to increasing C6 sugar content, decreasing xylan, and reducing lignin content can be combined in an additive manner. Thus, the engineered lines obtained by this trait-stacking approach have substantially improved properties from the perspective of biofuel production, and they do not show any obvious negative growth effects. The approach used in this study can be readily transferred to bioenergy crop plants.

Bioethanol production from lignocellulosic sugarcane leaves and tops

Bioethanol production is one of the most promising possible substitutes for fossil-based fuels, but there is a need to make available cost-effective methods of production if it is to be successful. Various methods for the production of bioethanol using different feedstocks have been explored. Bioethanol synthesis from sugarcane, their tops and leaves have generally been regarded as waste and discarded. This investigation examined the use of lignocellulosic sugarcane leaves and tops as biomass and evaluated their hydrolysate content. The leaves and tops were hydrolysed using concentrated and dilute sulphuric acid and compared with a combination of oxidative alkali-peroxide pre-treatment with enzyme hydrolysis using the enzyme cellulysin® cellulase. Subsequent fermentation of the hydrolysates into bioethanol was done using the yeast saccharomyces cerevisae. The problem of acid hydrolysis to produce inhibitors was eliminated by overliming using calcium hydroxide and this treatment was subsequently compared with sodium hydroxide neutralisation. It was found that oxidative alkali pre-treatment with enzyme hydrolysis gave the highest yield of fermentable sugars of 38% (g/g) for 7% (v/v) peroxide pretreated biomass than 36% (g/g) for 5% (v/v) with the least inhibitors. Concentrated and dilute acid hydrolysis each gave yields of 25% (g/g) and 22% (g/g) respectively, although the acid required a neutralisation step, resulting in dilution. Alkaline neutralisation of acid hydrolysates using sodium hydroxide resulted in less dilution and loss of fermentable sugars, compared with overliming. Higher yields of bioethanol of 13.7 g/l were obtained from enzyme hydrolysates than the 6.9 g/l ethanol from dilute acid hydrolysates. There was more bioethanol yield of 13.7 g/l after 72 hours of fermentation with the yeast than the 7.0 g/l bioethanol after 24 hours.This research showed that it is possible to use sugarcane waste material to supplement biofuel requirements and that combining the chemical and biological methods of pretreatments can give higher yields at a faster rate.

Sequential two-step fractionation of lignocellulose with formic acid organosolv followed by alkaline hydrogen peroxide under mild conditions to prepare easily saccharified cellulose and value-added lignin

For the production of the second generation bioethanol, enzymatic saccharification to prepare a high yield of fermentable sugars is an essential step for the conversion of energy from lignocellulose. In this case, a mild two-step pretreatment using anhydrous formic acid and alkaline hydrogen peroxide aqueous solution was developed to fractionate lignocellulose to highly digestible cellulose for enzymatic saccharification as well as value-added lignin. Bamboo was pretreated with anhydrous formic acid at 40–100°C for 4h and then extracted with alkaline hydrogen peroxide aqueous solution (containing 1% NaOH and 1% H2O2) at 80°C for 2h. The lignin dissolved in anhydrous formic acid was isolated and further treated with the alkaline solution obtained from the pretreatment and then recovered. The produced cellulose residue had a rather high conversion rate of cellulose into glucose (99.36%) under an enzymatic hydrolysis for 72h. In addition, the lignin obtained preserved good functionalities, which exhibited great potential to produce liquid fuels, thus implementing the philosophy of biorefinery. The results indicated that the two-step formic acid-alkaline hydrogen peroxide pretreatment effectively broke the recalcitrant nature of lignocellulose, producing a good feedstock for the conversion into energy.

Pretreatment optimization of the biomass of Microcystis aeruginosa for efficient bioethanol production

Microalgae are considered to be the future promising sources of biofuels and bio products. The algal carbohydrates can be fermented to bioethanol after pretreatment process. Efficient pretreatment of the biomass is one of the major requirements for commercialization of the algal based biofuels. In present study the microalga, M. aeruginsa was used for pretreatment optimization and bioethanol production. Treatment of algal biomass with CaO before acid and/or enzymatic hydrolysis enhanced the degradation of algal cells. Monomeric sugars yield was increased more than twice when biomass was pretreated with CaO. Similarly, an increase was noted in the amount of fermentable sugars when biomass was subjected to invertase saccharification after acid or lysozyme pretreatment. Highest yield of fermentable sugars (16 mM/ml) in the centrifuged algal juice was obtained. 4 Different microorganisms’ species were used individually and in combination for converting centrifuged algal juice to bioethanol. Comparatively higher yield of bioethanol (60 mM/ml) was obtained when the fermenter microorganisms were used in combination. The results demonstrated that M. arginase biomass can be efficiently pretreated to get higher yield of fermentable sugars for enhanced yield of bioethanol production.

Water Hyacinth as Non-edible Source for Biofuel Production

Water hyacinth (Eichhornia crassipes) represents a promising source for biofuel production and other bioactive compounds because of their high availability and high biomass yield. The present work aims to determine the possibility for biofuel production and other active compounds from seasonally collected water hyacinth. The water hyacinth samples were collected at four seasons (2014/2015) from River Nile, Giza, Egypt, and the biofuel (biodiesel and bioethanol), lipids, glycerol content, carbohydrates and other biochemical compounds were determined in addition to physico-chemically characterized of produced biodiesel The obtained results indicated that, water hyacinth samples showed variable lipid contents (6.79–10.45%), which by transesterification produced biodiesels (3.22–6.36%) and sediment (pigments+glycerol). Biodiesel composed either totally of saturated fatty acids (Myristic acid) of winter and autumn samples, However, Myristic and Stearic acids with small proportion of pentadecanoic acid of summer and spring samples by 8.1 and 7.9% respectively. The monounsaturated fatty acid, Oleic, was only recorded in the summer sample by11.6%. So biodiesels produced from water hyacinth have good stability and acceptability to be used in diesel engines, the co-products (sediment) composed of pigments and glycerol reached to 4.69 mg/g and 1.05 mmol/L respectively in winter season. Also, pretreatment of water hyacinth by acid at mild conditions was found to be effective with high yield of fermentable sugars and production of ethanol during 120–180 min for different seasonally collected plants. From the results, we can conclude that the produced biodiesel (from water hyacinth) was within the recommended standards and met the criteria required to be a diesel substitute compared with the Egyptian fuel standards In addition, it is possible to use its pigments as natural coloring substances in food industry, and glycerol can be incorporated in different petrochemical industries.