This experiment was conducted to determine the potency of essential oils derived from Zingiberaceae leaves as antibacterial against Streptococcus mutans and biofilm degradation on teeth. The essential oils were derived from Curcuma domestica, Curcuma zedoaria, Curcuma xanthorrhiza, Elettaria cardamomum, Kaempferia galanga, Zingiber officinale varietas rubrum, and Zingiber purpureum. The assay was performed through dilution method. Chlorhexidine and a commercial mouthwash were used as positive control. The highest yield of essential oil was obtained from Elettaria cardamomum (2.43%). Essential oil of Kaempferia galanga, Curcuma domestica, Elettaria cardamomum, and Zingiber purpureum were successfully inhibited Streptococcus mutans with a minimum inhibitory concentration of 2000 µg/mL. Furthermore, Elettaria cardamomum showed the best antibacterial activity and degradation of biofilms. This essential oil was further analyzed using gas chromatography-mass spectrometer. The results showed eucalyptol as the main compound responsible for antibacterial activity and was predicted to effect biofilm degradation activity.