High Voltage Electrical Discharges Research Articles

Plasma methods of obtainment of multifunctional composite materials, dispersion-hardened by nanoparticles

The new approach in developed plasma methods consists in that dispersionhardening additives (TiC, TiB2 in particular) are not mechanically added to powder mixture as additional component, as in conventional methods, but are instead synthesized during high voltage electric discharges (HVED) in disperse system “hydrocarbon liquid - powder”; preservation of ultrafine structure is ensured due to use of spark plasma sintering (SPS) as a consolidation method. HVED in disperse system “hydrocarbon liquid - powder” due to impact of plasma discharge channel, electromagnetic fields, shock waves mechanical impact, hydro flows and volume microcavitation leads to synthesis of nanocarbon, metal powders dispersion and synthesis of micro- (from 10-6 to 10-7 m) and nanosized (from 10-7 to 10-9 m) composite powders of hardening phases. SPS is the passage of pulsed current (superposition of direct and alternating current) through powder with the simultaneous mechanical compressing. The formation of plasma is initiated in gaseous phase that fills gaps between particles. SPS allows targeted control of grain growth rate and thus allows obtainment of multifunctional composite materials dispersion hardened by nanoparticles. Processes of HVED synthesis of micro- and nanosized powders of new compositions from elemental metal powders and their mixtures with the subsequent application of high-speed SPS of obtained powders create conditions for increase of strength (by 10 - 20%), hardness and wear-resistance (by 30 - 60%) of obtained materials.

Changes of Antioxidant Compounds in a Fruit Juice-Stevia rebaudiana Blend Processed by Pulsed Electric Technologies and Ultrasound

The purpose of this study was to compare the effects of the non-thermal technologies of pulsed electric fields (PEF), high-voltage electrical discharges (HVED) and ultrasound (USN) on bioactive compounds (ascorbic acid, total carotenoids, total phenolic compounds and total anthocyanins) and antioxidant capacity of a fruit juice (papaya and mango) blend sweetened with Stevia rebaudiana. Experiments were carried out at two equivalent energy inputs (32–256 kJ/kg) for each technology. Principal component analysis (PCA) was used to understand the contribution of ascorbic acid, total carotenoids and oxygen radical absorbance capacity (ORAC) values. These parameters were better retained with PEF treatments. Nevertheless, the use of HVED and USN technologies cannot be ruled out, as they may enhance the contents of other bioactive compounds such as total phenolic compounds when HVED technology is applied at an energy input of 256 kJ/kg. The obtained data can contribute to the determination of optimum processing conditions for production of high nutritional quality liquid foods.

Nitric Oxide Production by High Voltage Electrical Discharges for Medical Uses: A Review

Nitric oxide (NO) is a vasodilator and antihypertensive agent as well as a universal anti-microbial factor killing bacteria, fungi and parasites without killing human cells provided that an appropriate dose level and treatment time are applied. Exogenous NO is often employed in inhalation therapies for treating pulmonary hypertension in children and adults. NO generation from air in high voltage electrical discharges is being developed for medical uses because it is technologically simple, economical and portable. The related literature is reviewed here. The plasma can be a thermal plasma, where the temperature is of the order of 10,000 K, or it can be a non-thermal plasma, where the electron temperature is very high but the average gas temperature can vary over a wide range from close to room temperature to thousands of degrees above room temperature. The plasma temperature has significant effects on the chemical composition of the treated gas. These effects are explained based on the chemical reaction mechanism. Further, NO generated by electrical discharges is usually contaminated with nitrogen dioxide and sometimes with ozone and particulate matter. The techniques that have been successfully hybridized with the electrical discharge devices or that can potentially be hybridized for the purification of NO are also reviewed. Recent successful testing of electrical discharge-based NO generators for inhalation therapy on animal models in the US and routine use of them in Russia and east Europe for wound decontamination and fast heeling suggests that the technique has a great potential for applications in future.

New Approaches in Non-thermal Processes in the Food Industry

In terms of product quality and food safety biological and chemical activities must be brought under control and this is usually provided by traditional thermal processing. To provide thermal processes, enzymatic and microbiological inactivations in food are the most basic methods that are used. However, the height of the applied temperature has a negative impact on important parameters, which are the color of food, nutritional value, sensory characteristics and flavor. Therefore, in recent years, producers and consumers are engaged in healthy food in the quest to obtain the quality parameters with less deviation. From this point of view non-thermal new techniques have emerged. This study gives information about new food preservation techniques and the applications that can be used in the food industry. These are hover electric field, ultrasonication, high-pressure application, microfiltration, X-rays, ionizing radiation, high voltage electrical discharge, Pulsed Light, magnetic field heating and moderate magnetic field.

Impact of pulsed-electric field and high-voltage electrical discharges on red wine microbial stabilization and quality characteristics.

In this study, pulsed-electric fields (PEF) and high-voltage electrical discharges (HVED) are proposed as new techniques for the microbial stabilization of red wines before bottling. The efficiency of the treatment was then evaluated. PEF and HVED-treatments have been applied to wine for the inactivation of Oenococcus oeni CRBO 9304, O.oeni CRBO 0608, Pediococcus parvulus CRBO 2.6 and Brettanomyces bruxellensis CB28. Different treatment times (1, 2, 4, 6, 8 and 10ms) were used at 20kVcm(-1) for the PEF treatments and at 40kV for the HVED treatments, which correspond to applied energies from 80 to 800 kJl(-1) . The effects of the treatments on the microbial inactivation rate and on various characteristics of red wines (phenolic composition, chromatic characteristics and physico-chemical parameters) were measured. The application of PEF or HVED treatments on red wine allowed the inactivation of alteration yeasts (B.bruxellensis CB28) and bacteria (O.oeni CRBO 9304, O.oeni CRBO 0608 and P.parvulus CRBO 2.6). The electric discharges at 40 kV were less effective than the PEF even after 10ms of treatments. Indeed, 4ms of PEF treatment at 20kVcm(-1) were sufficient to inactivate all micro-organisms present in the wines. Also, the use of PEF had no negative impact on the composition of wines compared to the HVED treatments. Contrary to PEF, the phenolics compounds were degraded after the HVED treatment and the physico-chemical composition of wine were modified with HVED. PEF technology seems to be an interesting alternative to stabilize microbiologically wines before bottling and without modifying their composition. This process offers many advantages for winemakers: no chemical inputs, low energy consumption (320kJl(-1) ), fast (treatment time of 4ms) and athermal (ΔT≈10°C).

Microstructure Investigation of 13Cr-2Mo ODS Steel Components Obtained by High Voltage Electric Discharge Compaction Technique.

Refractory oxide dispersion strengthened 13Cr-2Mo steel powder was successfully consolidated to near theoretical density using high voltage electric discharge compaction. Cylindrical samples with relative density from 90% to 97% and dimensions of 10 mm in diameter and 10–15 mm in height were obtained. Consolidation conditions such as pressure and voltage were varied in some ranges to determine the optimal compaction regime. Three different concentrations of yttria were used to identify its effect on the properties of the samples. It is shown that the utilized ultra-rapid consolidation process in combination with high transmitted energy allows obtaining high density compacts, retaining the initial structure with minimal grain growth. The experimental results indicate some heterogeneity of the structure which may occur in the external layers of the tested samples due to various thermal and electromagnetic in-processing effects. The choice of the optimal parameters of the consolidation enables obtaining samples of acceptable quality.

Bioaccessibility of Bioactive Compounds and Antioxidant Capacityfrom Orange Peel after Pulsed Electric Fields and High Voltage Electrical Discharges

In the present study changes of bioactive compounds and antioxidant capacity in orange peel treated by pulsed electric fields (PEF), high voltage electrical discharges (HVED) at two equivalent energy inputs (55-364 kJ/kg) as well as relative bioaccessibility was evaluated. PEF treatment led to decrease in ascorbic acid when energy input increased. Moreover TPC after PEF and HVED treatments increased significantly with higher energy input applied. By contrast, HVED at 364 kJ/kg diminished carotenoids content. The relative bioaccessibility of carotenoids after HVED (55 kJ/kg)was 75.3%. When increasing the energy input from 55 to 364 kJ/kg in HVED the bioaccessibility percentage of carotenoids increased in a 82.5%. On the other hand TPC bioaccessibility for PEF at lower energy input was 40.7%. However increasing the energy input to 364 kJ/kg led to decrease of the TPC independently of treatment applied. Consequently, results indicated that non thermal treatments such as (PEF, HVED) can enhance the release of bioactive compounds and total antioxidant capacity after in vitrogastrointestinal digestion in orange peel. However, further research is required tooptimize processing conditions. In the present study changes of bioactive compounds and antioxidant capacity in orange peel treated by pulsed electric fields (PEF), high voltage electrical discharges (HVED) at two equivalent energy inputs (55-364 kJ/kg) as well as relative bioaccessibility was evaluated. PEF treatment led to decrease in ascorbic acid when energy input increased. Moreover TPC after PEF and HVED treatments increased significantly with higher energy input applied. By contrast, HVED at 364 kJ/kg diminished carotenoids content. The relative bioaccessibility of carotenoids after HVED (55 kJ/kg)was 75.3%. When increasing the energy input from 55 to 364 kJ/kg in HVED the bioaccessibility percentage of carotenoids increased in a 82.5%. On the other hand TPC bioaccessibility for PEF at lower energy input was 40.7%. However increasing the energy input to 364 kJ/kg led to decrease of the TPC independently of treatment applied. Consequently, results indicated that non thermal treatments such as (PEF, HVED) can enhance the release of bioactive compounds and total antioxidant capacity after in vitro gastrointestinal digestion in orange peel. However, further research is required tooptimize processing conditions.

Influence of Temperature and Solvent Content on Electrical Properties of Sunflower Seed Cake

Here, we compare the impact of solvent content and temperature on dielectric and conductivity properties of sunflower seed cake. Two different experimental conditions were adopted in this study: permittivity-frequency isothermal spectroscopy of sunflower seed cakes at different temperatures ranging from 30 to 45C; and impedance-frequency spectroscopy of sunflower seed cakes with various solvent contents, ranging from 10 to 50 wt%. Experimental results revealed that increasing solvent content or temperature of sunflower seed cake from 10 to 50 wt% and 30 to 45C, the conductivity of samples increase by around eight times (from 3.52 × 10−6 to 2.73 × 10−5 S/m) and six times (from 1.35 × 10−4 to 7.7 × 10−4 S/m), respectively. The maximum conductivity can be reached by addition of 50 wt% of solvent to sunflower seed cake and raising the temperature to about 45C. Desired electrical properties of sunflower seed cake can be obtained by optimizing its solvent content and the processing temperature. Practical Applications With the dependence equations obtained from the measured data at various temperatures and solvent content, it is possible for the optimization of process parameters for food industry-dependent technologies, which rely on electrical properties of process materials, such as pulsed electric field treatment (PEF), ultrasonic and high-voltage electric discharge for assisted oil extraction.

Densification of zirconium nitride by spark plasma sintering and high voltage electric discharge consolidation: A comparative analysis

The densification behavior of zirconium nitride powder is investigated for various temperature and pressure conditions imposed by spark plasma sintering and high voltage electric discharge consolidation techniques. The crystal structure, chemical composition, porosity, and grain size of the powders and processed specimens are analyzed by X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The outcomes of the two considered consolidation techniques are comparatively assessed. Vickers micro-hardness of the processed ZrN specimens is investigated, and hardness dependence on porosity is analyzed. The densification mechanism of ZrN consolidated by spark plasma sintering is revealed by applying the constitutive equation of the continuum theory of sintering, and it turns out to be a similar mechanism to hot pressing. Application of the sintering constitutive equations shows that the mechanism of ZrN densification by high voltage electric discharge consolidation method depends on the magnitude of the applied voltage.

Electrical, mechanical, and chemical effects of high-voltage electrical discharges on the polyphenol extraction from vine shoots

Abstract The aim of this work was to study the mechanical, electrical and chemical effects of high-voltage electrical discharges (HVED) and their role in polyphenol extraction from vine shoots. Cutting and grinding pretreatments were conducted prior to HVED treatment. Thus, different initial specific surface areas ( a 0 = 6, 8.5, 11, 84 and 1885.19 cm − 1 ) of vine shoots were tested. When the initial specific surface areas were low ( a 0 = 6, 8.5, 11 cm − 1 ), the HVED induced damage degrees ( D ) were the highest, leading to a better enhancement of polyphenol extraction. However, no product fragmentation was found for the lowest particle size ( a 0 = 1885.19 cm − 1 ) before and after the HVED treatment, and despite it, an amelioration of polyphenol content in extract of 20 mg/L was observed. The electrical arc generated H 2 O 2 but did not lead to polyphenol degradation. Industrial relevance This study gives significant information about the action mechanisms of high voltage electrical discharge treatment presenting relevant data for the design of the process. It shows the effectiveness of electrical discharges for the intensification of the extraction of bioactive molecules (polyphenols) from a specific byproduct (vine shoots). The paper also presents an energy-saving extraction process, relevant for industrial application, since no organic solvents were used. Process duration and temperature were also reduced.

Extraction assisted by pulsed electric energy as a potential tool for green and sustainable recovery of nutritionally valuable compounds from mango peels

The study compares the efficiency of conventional aqueous extraction at different temperatures (20–60°C) and pH (2.5–11) and extraction assisted by pulsed electric energy (pulsed electric fields, PEF or high voltage electrical discharges, HVED) of nutritionally valuable compounds found in mango peels. Exponential decay pulses with initial electric field strengths of ≈13.3kV/cm and ≈40kV/cm for PEF and HVED treatments were used, respectively. The impact of temperature on aqueous extraction of proteins and carbohydrates was not significant. The highest values of nutritionally valuable and antioxidant compounds (7.5mM TE) were obtained for aqueous extraction (T=60°C, pH 6) but extracts were unstable and cloudy. The application of two-stage procedure PEF+supplementary aqueous extraction (+SE) that include PEF-assisted extraction as the first step, and +SE at 50°C, pH 6 during 3h as the second step, allowed a noticeable enhancement of the yields of TPC (+400%) even at normal pH.

Emerging technologies for the recovery of isothiocyanates, protein and phenolic compounds from rapeseed and rapeseed press-cake: Effect of high voltage electrical discharges

Abstract The aim of the present research study was to gain insight into the applicability of high voltage electrical discharges (HVED) technology for obtaining high-added value compounds (protein, polyphenols and isothiocyanates) from rapeseeds and rapeseed press-cake. For this purpose, HVED treatment was applied at different energy inputs (0–400 kJ/kg) to raw rapeseed and rapeseed press-cake water suspensions at different liquid-to-solid ratios (1:5–1:20, w/w). The obtained results clearly show the high potential of HVED to recover isothiocyanates, phenolic compounds and proteins which can be used as food additives and/or nutraceuticals. In almost all cases, an optimal treatment energy input (80 kJ/kg or 240 kJ/kg) was observed beyond which the content of the biocompounds decreases. This optimal energy input was changing depending on the considered compounds and on the raw materials. For example, for polyphenols, the optimal energy was 80 kJ/kg with rapeseed press-cake, but it was 240 kJ/kg with rapeseeds.

Comparison of the effect of pulsed electric field or high voltage electrical discharge for the control of sweet white must fermentation process with the conventional addition of sulfur dioxide

The present work discusses the efficiency of pulsed electrical treatments for the inactivation of yeasts. The application of pulsed electric fields (PEFs) and high voltage electrical discharges (HVEDs) as alternatives to sulfites, which are used as anti-microbial to stop the fermentation of sweet white wine, was investigated. The influence of sulfite concentration (from 0mg·L−1 to 500mg·L−1), PEF (from 4kV·cm−1 to 20kV·cm−1; from 0.25ms to 6ms) and HVED (40kV/cm; 1ms or 4ms) treatments on the inactivation of total yeasts and non-Saccharomyces yeasts was determined. The addition of SO2 (250mg·L−1) resulted in 8 log total yeast reduction. The maximum yeast inactivation obtained with PEF and HVED was respectively 3 and 4 logs. The use of SO2, HVED and PEF allows decreasing the non-Saccharomyces yeast level by 7, 5 and 4 logs respectively. However, the wine browning was less pronounced for the samples treated by PEF in comparison with HVED and SO2 treatments. PEF seems to be the most suitable alternative technique to sulfite addition.

Effect of alternative physical pretreatments (pulsed electric field, high voltage electrical discharges and ultrasound) on the dead-end ultrafiltration of vine-shoot extracts

This work is devoted to the dead-end ultrafiltration of vine shoot extracts; these extracts were obtained after a previous step of solid–liquid extraction assisted by different physical pretreatments. Grinding, high-voltage electrical discharges (HVED), pulsed electric fields (PEF) and ultrasounds (US) were studied. The highest cellular damage (Z) was provoked by grinding (360kJ/kg; Z=1) then HVED (242kJ/kg; Z=0.71). The resulting protein and polyphenol concentrations were 0.097 and 0.4mg/mL for grinding and 0.082 and 0.3mg/mL after HVED pretreatment. A relationship between the extraction process and the unstirred dead-end ultrafiltration parameters was found. The highest cellular damage obtained with grinding (Z=1) gave the greatest specific cake resistances (αc≈4.8×1013m/kg). A correlation was therefore observed between cellular damage resulting in a higher polyphenol concentration and the membrane fouling phenomenon. HVED treatment (242kJ/kg) gave a lower specific cake resistance (αc≈4×1013m/kg) than grinding. Dynamic ultrafiltration was also conducted to decrease membrane fouling and study polyphenol purification and concentration in retentates. The experimental conditions leading to the highest filterability (highest flux J) and polyphenol retention conducted on HVED (242kJ/kg) extracts were a stirring velocity of 150rpm, a pressure value of 4.5×105Pa and a nominal molecular weight cut-off equal to 50kDa for the polyethersulfone membrane. Under these conditions, the filterability of HVED extracts (2.7×10−6m3/m2s) was higher than that of grinding (2×10−6m3/m2s). Up to 8% of polyphenols from the grinding extract were shown to deposit on the membrane. A lower percentage was found for HVED (7%). Polyphenols were therefore shown to be implicated in membrane fouling and pore blocking for unstirred and dynamic ultrafiltration processes.

New approaches for the effective valorization of papaya seeds: Extraction of proteins, phenolic compounds, carbohydrates, and isothiocyanates assisted by pulsed electric energy

The study compares the efficiency of common aqueous extraction (CE) at different pH (2.5–11) and temperatures (20–60°C) and extraction assisted by pulsed electric energy (pulsed electric fields, PEF or high voltage electrical discharges, HVED) of nutritionally valuable and antioxidant compounds from papaya seeds. The exponential decay pulses with initial electric field strengths of ≈13.3kV/cm and ≈40kV/cm for PEF and HVED treatments, respectively, were used. The number of pulses n was changed within 1–2000. The impacts of temperature and pH on extraction efficiency of different components (proteins, total phenolic compounds, carbohydrates, isothiocyanates) and antioxidant capacity were ambiguous. The highest values of nutritionally valuable and antioxidant compounds were obtained for HVED-assisted extraction. However, the application of HVED-treatment may produce undesirable contaminants (chemical products of electrolysis, free reactive radicals, etc.) and extracts were unstable and cloudy. On the other hand, the application of the two-stage procedure PEF+ supplementary aqueous extraction (+SAE) that include PEF-assisted extraction as the first step, and +SAE at 50°C, pH=7 during 3h as the second step, allowed noticeable enhancement of the yields (+200%) and antioxidant capacities (+20%) even at neutral pH. This method has high prospects of industrial applications for release of valuable components from papaya seeds.

Unique patterns of transcript and miRNA expression in the South American strong voltage electric eel (Electrophorus electricus).

BackgroundWith its unique ability to produce high-voltage electric discharges in excess of 600 volts, the South American strong voltage electric eel (Electrophorus electricus) has played an important role in the history of science. Remarkably little is understood about the molecular nature of its electric organs.ResultsWe present an in-depth analysis of the genome of E. electricus, including the transcriptomes of eight mature tissues: brain, spinal cord, kidney, heart, skeletal muscle, Sachs’ electric organ, main electric organ, and Hunter’s electric organ. A gene set enrichment analysis based on gene ontology reveals enriched functions in all three electric organs related to transmembrane transport, androgen binding, and signaling. This study also represents the first analysis of miRNA in electric fish. It identified a number of miRNAs displaying electric organ-specific expression patterns, including one novel miRNA highly over-expressed in all three electric organs of E. electricus. All three electric organ tissues also express three conserved miRNAs that have been reported to inhibit muscle development in mammals, suggesting that miRNA-dependent regulation of gene expression might play an important role in specifying an electric organ identity from its muscle precursor. These miRNA data were supported using another complete miRNA profile from muscle and electric organ tissues of a second gymnotiform species.ConclusionsOur work on the E. electricus genome and eight tissue-specific gene expression profiles will greatly facilitate future research on determining the coding and regulatory sequences that specify the function, development, and evolution of electric organs. Moreover, these data and future studies will be informed by the first comprehensive analysis of miRNA expression in an electric fish presented here.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1288-8) contains supplementary material, which is available to authorized users.

Effect of pulsed electric fields and high voltage electrical discharges on polyphenol and protein extraction from sesame cake

Abstract The study evaluates the effect of pulsed electric fields (PEF) and high voltage electrical discharges (HVED) on the extraction of sesame cake compounds. Different energy inputs were applied and, afterwards, PEF and HVED were used as pre-treatments to diffusion. Diffusion kinetics were evaluated in diverse ethanol concentrations and temperatures. The extracts were analyzed for polyphenols, lignans and proteins. According to the results, the disintegration index and the polyphenol and protein contents increased with the energy inputs, until 83 kJ/kg was reached. During diffusion, both pre-treatments improved extraction yields of polyphenols and lignans through different solvents. For diffusion in different temperatures, PEF and HVED had a significant positive effect on the extraction of proteins and polyphenols when compared to the control sample. Results show that the use of PEF or HVED can reduce the use of organic solvents and the need for high temperatures to improve diffusion. Industrial relevance There is an increasing demand by the industry for the development of extraction procedures that reduce or eliminate the need for organic solvents and high temperatures. Also, food producers are aiming to find new application for by-products from the industry. PEF and HVED assisted extraction damages the product cells, increasing diffusion from the product to the solvent. The concentration of polyphenols and protein in the solvent after PEF and HVED treatments shows that the use of these techniques is relevant for an industrial use, since solvent amount and extraction time and temperature can be reduced.

Design and construction of prototype transversely excited atmospheric (TEA) nitrogen laser energized by a high voltage electrical discharge

The present study reports design and construction, of a prototype of Transversely Excited Atmospheric (TEA) nitrogen laser, and a high voltage power supply to excite N2 gas in air, while air is used as an active lasing medium. A Blumlein line discharge circuit is used for operation of this laser. The high voltage is generated by a fly back transformer based power supply varying from 10kV to 20kV. The wavelength (337.1nm) of TEA nitrogen laser is measured employing a standard commercial spectrometer and the laser output energy of 300μJ is measured from the constructed system. Different parameters such as beam profile, laser output spectrum, laser efficiency, and variation of E/P (Electrical field/Pressure) value with respect to input voltage and electrodes separations are studied in order to optimize the overall operational efficiency of present nitrogen laser. The analysis of the high voltage prototype appeared in this designed source has also been made and described in this paper.

Effect of Alternative Physical Treatments (Ultrasounds, Pulsed Electric Fields, and High-Voltage Electrical Discharges) on Selective Recovery of Bio-compounds from Fermented Grape Pomace

The aim of this study is to compare alternative treatments on solvent-free extraction of high added value components from fermented grape pomace. Ultrasounds (US), pulsed electric fields (PEF) and high voltage electric discharges (HVED), which are physical treatments able to induce cell damages, were applied on aqueous suspensions of grape pomace. The efficiency of these technologies for phenolic compounds extraction, and particularly for anthocyanins recovery, was evaluated throughout the treatments at equivalent cell disintegration indexes (Z). HVED proved to be the most interesting technique to achieve higher phenolic compounds recovery with lower energy requirement than PEF and US at the same values of Z. However, HVED was less selective than PEF and US regarding the amount of anthocyanins recovered. At equivalent cell disintegration of Z = 0.8, PEF remarkably increased the extraction yield of total anthocyanins up to 22 and 55 % in comparison with US and HVED-assisted extractions. At this Z value, the ratio of total anthocyanins to TPC extracted reaches the respective values of 41.7, 34.9 and 14.1 % for PEF, US and HVED, thus demonstrating interesting differences of selectivity of the treatments.

Potential use of pulsed electric technologies and ultrasounds to improve the recovery of high-added value compounds from blackberries

A better knowledge of the effect of non-conventional extraction technologies, which can avoid the use of high temperatures and toxic solvents, on the antioxidant compounds recovery from blackberries, is necessary. Thus, high voltage electrical discharges (HVED), pulsed electric fields (PEF), and ultrasounds (USN) treatments were applied in order to evaluate the effects of processing on protein, total phenolics and anthocyanins extraction from blackberries. Moreover, two-stage extraction involving the use of HVED, PEF and USN as pre-treatments and supplementary extraction during 5h with hot water at mild temperature (50°C) or an hydroalcoholic solution (30% ethanol, w/w) was evaluated. The impacts of both hot water (50°C) and ethanol had a positive influence in total phenolics and anthocyanin recovery, although the yield was clearly influenced by the treatment applied and the targeted compound. The highest protein (37.95±1.30mg/100g) and TPC (932.69±33.45mg/100g) yields were obtained after HVED and supplementary extraction during 5h, using ethanol and hot water at 50°C, respectively. However, the maximum anthocyanin yield (98.46±4.92mg/100g) was found after applying PEF treatment and supplementary extraction with hot water at 50°C. These promising findings open the doors to a potential multi-stage green recovery, which can improve the selectivity and the amount of high-added value compounds recovered.