High Trans Fat Research Articles

Compound Molecules of Network Pharmacology-Based of Tamarillo (Cyphomandra betacea Cav.) and the Potential as Noodle for Type 2 Diabetes Mellitus Treatment

Consuming noodles excessively is not good for health because it contains high trans fat, which can cause diabetes mellitus (DM) as a disease with a high prevalence, especially type 2 DM, as much as 95% in Indonesia. Tamarillo (Chypomandra betacea Cav.) can potentially reduce blood glucose levels and restore adipokine regulation to prevent insulin resistance in type 2 DM. This study aims to determine the formulation and potential of tamarillo compounds into noodle products named "Chypotes (Chypomandra Antidiabetes) Noodle" as an alternative food for type 2 DM patients. The methods used are noodle formulation, organoleptic test, hedonic test, and network pharmacology. Based on the results of an organoleptic test, Chypotes Noodle has a chewy texture, a distinctive smell, yellow colour, and a slightly sour tasteless taste typical of Tamarillo. Based on the results of the hedonic test, 30 respondents chose the like and very like options in filling out the questionnaire with a percentage of 80-90%. Based on the Network Pharmacology of Tamarillo shows that 150 of the 365 target proteins of Tamarillo are involved in the biological processes and signaling pathways of type 2 diabetes mellitus, with 75 target proteins locking and interacting. Compound Molecules of Tamarillo, such as six bioactive components of the hydroxycinnamic acids group, have activity in type 2 DM and affect the biological processes and signaling pathways of type 2 DM against ADIPOQ, TNF, and INS. Therefore, Tamarillo can potentially be used as an alternative food product in the form of noodles for type 2 DM treatment.

A high-trans fat, high-carbohydrate, high-cholesterol, high-cholate diet-induced nonalcoholic steatohepatitis mouse model and its hepatic immune response

Non-alcoholic fatty liver disease (NAFLD) is a chronic progressive disease that can progress to non-alcoholic steatohepatitis (NASH). Animal models are important tools for basic NASH research. Immune activation plays a key role in liver inflammation in patients with NASH. We established a high-trans fat, high-carbohydrate, and high-cholesterol, high-cholate diet-induced (HFHCCC) mouse model. C57BL/6 mice were fed a normal or HFHCCC diet for 24 weeks, and the immune response characteristics of this model were evaluated. The proportion of immune cells in mouse liver tissues was detected by immunohistochemistry and flow cytometry, Multiplex bead immunoassay and Luminex technology was used to detecte the expression of cytokines in mouse liver tissues. The results showed that mice treated with HFHCCC diet exhibited remarkably increased hepatic triglycerides (TG) content, and the increase in plasma transaminases resulted in hepatocyte injury. Biochemical results showed that HFHCCC induced elevated hepatic lipids, blood glucose, insulin; marked hepatocyte steatosis, ballooning, inflammation, and fibrosis. The proportion of innate immunity-related cells, including Kupffer cells (KCs), neutrophils, dendritic cells (DCs), natural killer T cells (NKT), and adaptive immunity-related CD3+ T cells increased; interleukin-1α (IL-1α), IL-1β, IL-2, IL-6, IL-9, and chemokines, including CCL2, CCL3, and macrophage colony stimulating factor (G-CSF) increased. The constructed model closely approximated the characteristics of human NASH and evaluation of its immune response signature, showed that the innate immune response was more pronounced than adaptive immunity. Its use as an experimental tool for understanding innate immune responses in NASH is recommended.

Healthy lifestyles and wellbeing reduce neuroinflammation and prevent neurodegenerative and psychiatric disorders.

Since the mid-20th century, Western societies have considered productivity and economic outcomes are more important than focusing on people's health and wellbeing. This focus has created lifestyles with high stress levels, associated with overconsumption of unhealthy foods and little exercise, which negatively affect people's lives, and subsequently lead to the development of pathologies, including neurodegenerative and psychiatric disorders. Prioritizing a healthy lifestyle to maintain wellbeing may slow the onset or reduce the severity of pathologies. It is a win-win for everyone; for societies and for individuals. A balanced lifestyle is increasingly being adopted globally, with many doctors encouraging meditation and prescribing non-pharmaceutical interventions to treat depression. In psychiatric and neurodegenerative disorders, the inflammatory response system of the brain (neuroinflammation) is activated. Many risks factors are now known to be linked to neuroinflammation such as stress, pollution, and a high saturated and trans fat diet. On the other hand, many studies have linked healthy habits and anti-inflammatory products with lower levels of neuroinflammation and a reduced risk of neurodegenerative and psychiatric disorders. Sharing risk and protective factors is critical so that individuals can make informed choices that promote positive aging throughout their lifespan. Most strategies to manage neurodegenerative diseases are palliative because neurodegeneration has been progressing silently for decades before symptoms appear. Here, we focus on preventing neurodegenerative diseases by adopting an integrated "healthy" lifestyle approach. This review summarizes the role of neuroinflammation on risk and protective factors of neurodegenerative and psychiatric disorders.

PRODUCTOS ULTRAPROCESADOS EN ARGENTINA: EVALUACIÓN DEL MODELO DE PERFIL DE NUTRIENTES DE LA ORGANIZACIÓN PANAMERICANA DE LA SALUD

Introduction: In Argentina, problems related to excess weight constitute one of the main challenges for public health. The background indicates that the consumption of ultra-processed products contributes to this trend due to their high sugar, total saturated, trans fat and sodium content. This study analyzes the PAHO nutrient profile in some ultra-processed products available in the Argentine market. Materials and methods: Field, cross-sectional, comparative study. Nutrient profile (free sugars, total fat, saturated fat, trans fat, sodium) and caloric density were evaluated. The categories were: sweet cookies, alfajores, chocolates, candies, ice creams, cereals, drinks, spreads, salty and dairy snacks. A descriptive and inferential statistical analysis was performed. Results: The sample was made up of 682 products and all of them exceeded the cut-off point of at least one nutrient, 94.4% presented an excessive amount of free sugars, 47.9% an excessive amount of total fats, 59. 2% excessive amount of saturated fat, 10.6% excessive amount of trans fat and 9.1% excessive amount of sodium. The average of the total caloric density was 3.19. Products with an excessive amount of total, saturated and trans fats had a higher caloric density (p<0.05); however, the caloric density of the products with an excessive amount of free sugars was lower (p=0.000), the same trend was found in the case of sodium, but this difference was not statistically significant. Conclusions: All the products exceeded the cut-off point for at least one critical nutrient and having a lower caloric density did not guarantee their nutritional quality. The nutrient profile accurately reflects the nature of ultra-processed products. Keywords: Ultra-Processed foods; food labeling; PAHO; Argentina.

Food security and diet quality in a racially diverse cohort of postpartum women in the USA.

Food insecurity has been associated with poor diet, but few studies focused on the postpartum period - an important time for women's health. We examined associations between food security and diet quality in postpartum women and assessed whether participation in federal food assistance programmes modified this potential relation. Using longitudinal data, we analysed the association between food security at 3 months postpartum and a modified Alternate Healthy Eating Index-2010 (AHEI) at 6 months postpartum (excluding alcohol). We conducted multivariable linear regressions examining associations between food security and AHEI. We assessed two food assistance programmes as potential effect modifiers. The sample included 363 postpartum women from the Nurture study, located in the Southeastern USA (2013-2017). Among women, 64·4 % were Black and 45·7 % had a high school diploma or less. We found no evidence of an interaction between food security and two federal food assistance programmes. In adjusted models, marginal, low and very low food security were not associated with AHEI. However, low (β: -0·64; 95 % CI -1·15, -0·13; P = 0·01) and very low (β: -0·57; 95 % CI -1·02, -0·13; P = 0·01) food security were associated with greater trans fat intake. Food security status was not associated with overall diet quality but was associated with higher trans fat (low and very low) and more moderate alcohol (marginal) intake. Future studies should assess the consistency and generalisability of these findings.

The Formation of the Criteria, Rules and Regulations of the Sufficient and High-Quality Food According to the European Standards

Abstract. Introduction. Modern food systems face the challenge of supporting food choices that are consistent with a healthy and adequate diet. The vast majority of consumers of food products do not choose the principle of quality � the choice is made in favor of lower cost (low nutrient content), readiness of the product for consumption (high processing and trans fat content) or taste (high sugar, salt, etc.). At the same time, poor nutrition around the world is the number one risk factor for death and disability. Purpose. To study the criteria of the sufficient and high-quality food based on the norms and standards according to the European standards at different levels. Results. The article examines the indicators of calorie consumption per capita, daily calorie reserve per person, the minimum daily calorie requirement, inequality in calorie consumption per capita in the world. The key drivers of the impact of the level of nutrition on the quality of life have been identified: a healthy diet; unhealthy diet and lack of physical activity are the main global health risks, especially obesity; breastfeeding; balanced energy consumption; restriction of sugar consumption (up to 10%) and salt (up to 5 g per day). The WHO data and recommendations on ensuring a healthy diet for adults and children are summarized. The effective actions of politicians to create a healthy food environment have been identified, which should include three interrelated areas: building coherence in national policies and investment plans, including trade, food and agricultural policies; encouraging consumer demand for healthy food; promoting appropriate feeding practices for infants and young children; and tools for their implementation at the national level were systematized. WHO�s healthy eating policies and programs for national governments and related healthy eating policies and programs in Ukraine were described. Conclusions. A healthy diet from birth and throughout life prevents malnutrition in all its forms, as well as a number of non-communicable diseases. However, increased production and consumption of processed foods, rapid urbanization and lifestyle changes have led to changes in diets. People now consume significantly more foods high in energy, fat, sugar and salt / sodium. At the same time many people do not consume enough fruits, vegetables and whole grains, which contain the necessary fiber, vitamins and minerals for the human body. The required content of a varied, balanced and healthy diet varies depending on individual characteristics (age, gender, lifestyle and level of physical activity), cultural context, local products and eating habits. Along with this the basic principles of healthy nutrition remain unchanged.

Fat quality of ready to eat foods without nutritional label from unorganized sector

The aimofthis studywas to investigate total fat and fatty acid composition in processed foods fromunorganized sector (without nutritional labels), with emphasis on trans fatty acid (TFA) content. A total of 41 products, dividedinto 5 categories, viz., salted snack foods (n=9), sweets (n=7), street foods (n=10), meal items (n=4) baked foods (n=11) were sampled for estimation of fatty acid composition. Results indicate mean total fat content in all foodsranged between 16.88 to 30.64%, with high saturated (36.14 to 58.20%) and trans fat content (1.18 to 3.40%). Palmitic acid (C16:0) was the highest fatty acid varying between 17.01% - 46.80% in all the processed foods, followed by Oleic acid (C18:2, n6cis). Elaidic acid (C18:1, n9trans), Linolelidic acid (C18:2, n6trans) and Vaccenic acid (C18:1, n11trans) were the trans fatty acids found in 93% of all the foods estimated. The quality of fats in most of the foods analyzed was found to be unhealthy due to high TFA content, which is a possible contributing risk factor for diet-related non-communicable diseases, and the consumers are unaware of the fat quality due to absenceof nutritional labels.

Evaluation of Trans Fat in Vermicelli by Fourier Transform Infrared Spectroscopy in Combination with Chemometric Technique

Since there is limited knowledge about trans fat levels in snack foods available in Pakistan. This study aimed to determine trans fat content in fried vermicelli (a common snack food). It is prepared in partially hydrogenated oil (ghee), which is the main cause of trans fat formation. Twenty-one fried vermicelli samples were collected from different local shops of Hyderabad, Pakistan and subjected to oil extraction by using Soxhlet method. Partial least square regression model was developed to quantify trans fats in fried vermicelli using FTIR spectroscopy. Total oil content in fried vermicelli ranged between 17.28- 32.99%. FTIR results revealed that fried vermicelli showed trans fats in between 7.3-20.9% in extracted oil, whereas total trans fat in food was ranged at 1.3-5.9%. The FTIR data of trans fat was compared with GC-MS data and found comparable results (pandgt;0.05). Presence of high trans fat in fried vermicelli is an alarming issue for the health of consumers.

Evaluation of Trans Fat in Vermicelli by Fourier Transform Infrared Spectroscopy in Combination with Chemometric Technique

Since there is limited knowledge about trans fat levels in snack foods available in Pakistan. This study aimed to determine trans fat content in fried vermicelli (a common snack food). It is prepared in partially hydrogenated oil (ghee), which is the main cause of trans fat formation. Twenty-one fried vermicelli samples were collected from different local shops of Hyderabad, Pakistan and subjected to oil extraction by using Soxhlet method. Partial least square regression model was developed to quantify trans fats in fried vermicelli using FTIR spectroscopy. Total oil content in fried vermicelli ranged between 17.28- 32.99%. FTIR results revealed that fried vermicelli showed trans fats in between 7.3-20.9% in extracted oil, whereas total trans fat in food was ranged at 1.3-5.9%. The FTIR data of trans fat was compared with GC-MS data and found comparable results (pandgt;0.05). Presence of high trans fat in fried vermicelli is an alarming issue for the health of consumers.

Physicochemical characterization of shortenings recommended for application in cookie filling

Shortening is the commercial name given to a continuous-phase fat used in fried foods, baked goods, fillings, and other. This study aimed to evaluate commercial shortenings for cookie filling, assessing how these samples are composed in face of the new nutritional changes in the area and attempting to devise a technological profile of these fats for a further development of zero trans and low saturated bases. Six samples of shortenings for filling were evaluated regarding their composition in fatty acids, solid fat content, melting point, crystallization isotherms, thermal behavior, texture, and polymorphism. We observed there are still samples with high trans fat content in the market, but there are also samples low on these fatty acids; however, a direct exchange for saturated fatty acids (mainly palmitic acid) was verified. Shortenings for cookie filling should both have a palmitic acid content ~40%; SFC between 15-25% at process temperature; melting range between 35-55°C, with melting point from 42 to 46°C; average crystalline diameter of spherulite crystals between 12-15µm; Beta-polymorphic, and consistency and adhesion between 4000-1000 gF/cm² and 70-20 gF.s, respectively.

Transgenerational impact of maternal obesogenic diet on offspring bile acid homeostasis and nonalcoholic fatty liver disease.

Studies show maternal obesity is a risk factor for metabolic syndrome and nonalcoholic fatty liver disease (NAFLD) in offspring. Here we evaluated potential mechanisms underlying these phenotypes. Female C57Bl6 mice were fed chow or an obesogenic high-fat/high-sucrose (HF/HS) diet with subsequent mating of F1 and F2 female offspring to lean males to develop F2 and F3 generations, respectively. Offspring were fed chow or fibrogenic (high transfat, cholesterol, fructose) diets, and histopathological, metabolic changes, and bile acid (BA) homeostasis was evaluated. Chow-fed F1 offspring from maternal HF/HS lineages (HF/HS) developed periportal fibrosis and inflammation with aging, without differences in hepatic steatosis but increased BA pool size and shifts in BA composition. F1, but not F2 or F3, offspring from HF/HS showed increased steatosis on a fibrogenic diet, yet inflammation and fibrosis were paradoxically decreased in F1 offspring, a trend continued in F2 and F3 offspring. HF/HS feeding leads to increased periportal fibrosis and inflammation in chow-fed offspring without increased hepatic steatosis. By contrast, fibrogenic diet-fed F1 offspring from HF/HS dams exhibited worse hepatic steatosis but decreased inflammation and fibrosis. These findings highlight complex adaptations in NAFLD phenotypes with maternal diet.

AIM-deficient mouse fed a high-trans fat, high-cholesterol diet: a new animal model for nonalcoholic fatty liver disease.

Owing to changes in lifestyle, nonalcoholic fatty liver disease (NAFLD) is becoming a common form of chronic liver injury. NAFLD comprises a wide variety of disease stages, from simple steatosis to nonalcoholic steatohepatitis, which is a risk factor for the development of hepatocellular carcinoma (HCC). Because animal models for NAFLD are needed to investigate the precise pathogenesis, we aimed to establish a new mouse model employing mice deficient for apoptosis inhibitor of macrophage (AIM−/−), which exhibit accelerated lipid storage in the liver and high susceptibility to developing HCC in response to a high-fat diet (HFD). AIM−/− mice were fed the D09100301 diet, which contains 40 kcal% fat (trans fat 30 kcal%), high cholesterol (2%), and 40 kcal% carbohydrates (20 kcal% fructose), and then features of obesity and NAFLD including steatosis, inflammation, fibrosis, and HCC development were analyzed. Although a comparable grade of liver steatosis was promoted in AIM−/− mice by the D09100301 diet and the standard HFD (60 kcal% largely lard fat), significantly less lipid storage in visceral fat was observed when the mice were fed the D09100301 diet. Accelerated liver inflammation was promoted by the D09100301 diet compared with the HFD, but interestingly, HCC development was decreased in mice fed the D09100301 diet. Our findings suggest that AIM−/− mice fed the D09100301 diet exhibited a phenotype that resembled nonobese NAFLD patients and thus could be an appropriate tool to study the pathophysiology by which obesity increases the risk of HCC.

Socioeconomic Inequities in Diet Quality and Nutrient Intakes among Australian Adults: Findings from a Nationally Representative Cross-Sectional Study.

Poor diet may represent one pathway through which lower socioeconomic position (SEP) leads to adverse health outcomes. This study examined the associations between SEP and diet quality, its components, energy, and nutrients in a nationally representative sample of Australians. Dietary data from two 24-h recalls collected during the cross-sectional Australian Health Survey 2011-13 (n = 4875; aged ≥ 19 years) were analysed. Diet quality was evaluated using the Dietary Guidelines Index (DGI). SEP was assessed by index of area-level socioeconomic disadvantage, education level, and household income. Linear regression analyses investigated the associations between measures of SEP and dietary intakes. Across all of the SEP indicators, compared with the least disadvantaged group, the most disadvantaged group had 2.5–4.5 units lower DGI. A greater area-level disadvantage was associated with higher carbohydrate and total sugars intake. Lower education was associated with higher trans fat, carbohydrate, and total sugars intake and lower poly-unsaturated fat and fibre intake. Lower income was associated with lower total energy and protein intake and higher carbohydrate and trans fat intake. Lower SEP was generally associated with poorer diet quality and nutrient intakes, highlighting dietary inequities among Australian adults, and a need to develop policy that addresses these inequities.

APD668, a G protein-coupled receptor 119 agonist improves fat tolerance and attenuates fatty liver in high-trans fat diet induced steatohepatitis model in C57BL/6 mice

G-protein coupled receptor 119 (GPR119) receptor is a rhodopsin-like, class A Gαs-coupled receptor, predominantly expressed in pancreatic islet cells and intestinal entero-endocrine cells. GPR119 has been emerged as a novel therapeutic target for the treatment of dyslipidemia in type 2 diabetes. In this study, we investigated the effect of APD668, a GPR119 agonist alone and in combination with linagliptin, a DPPIV inhibitor on oral fat tolerance test. Our findings demonstrate that APD668, a GPR119 agonist inhibits the intestinal triglyceride absorption after acute fat load in mice. Single dose administration of APD668 increases incretin secretion and enhances total PYY levels in presence of fat load in mice. We found that, the anti-dyslipidemic action of APD668 was reversed in presence of exendin-3 in oral fat tolerance test. In addition, our results showed that exendin-3 (9−39) failed to block the effect of APD668 on gastric emptying indicating that gastric emptying effects of APD668 are indeed mediated through GPR119 receptor dependent mechanism. Combined administration of APD668 and linagliptin significantly increased plasma active GLP-1 levels in-vivo and showed improvement in fat tolerance. However, APD668 failed to show anti-dyslipidemic activity in tyloxapol-induced hyperlipidemia in mice. Furthermore, we investigated the chronic effects of APD668 on hepatic steatosis in high trans-fat diet fed steatohepatitis model in mice. Oral administration of APD668 in HTF diet fed mice ameliorated hepatic endpoints such as plasma ALT, AST, liver weight and steatosis. These findings suggest that GPR119 agonists may represent a promising therapeutic strategy for the treatment of dyslipidemia and non-alcoholic steatohepatitis.

424 - Advanced Glycation End Products Inhibition Restores Sphingolipid Rheostat in Mice Liver and Prevents Diet-Induced Insulin Resistance

The maintenance of sphingolipid rheostat by interconversion of ceramide (Cer), sphingosine (Sph), and sphingosine-1-phosphate (S1P) is relevant for the control of several metabolic pathways. In particular, accumulation of Cer and S1P in response to cellular stress interferes with insulin signalling and mitochondrial metabolism. Emerging evidence has highlighted a role for Advanced Glycation End Products (AGEs) in the imbalance of the sphingolipid rheostat. We and others have recently demonstrated the accumulation of AGEs in tissues of diet-induced insulin-resistant mice and their involvement in altered lipid synthesis and mitochondrial dysfunction. We, thus, investigated the impact of a high trans fat diet on the sphingolipid rheostat in mice liver and whether the administration of the anti-AGEs compound pyridoxamine could prevent insulin resistance onset by restoring the Cer/Sph/S1P equilibrium. C57Bl/6J mice were fed a standard diet (SD) or a 60% trans fat diet (HFD) for 10 weeks. Two subgroups received pyridoxamine (150 mg/kg/day) in the drinking water. The pyridoxamine administration efficiently prevented insulin resistance, obesity, AGEs accumulation and RAGE hyperexpression induced by the HFD. HFD also induced a dramatic reduction of the liver Sph levels, paralleled by the increase in Cer levels, assessed by an UPLC/TQMS system, and the induction of the S1P receptors. Interestingly, pyridoxamine administration prevented the Cer increase by reducing Cer synthase expression and significantly inhibited S1P receptors by reducing S1P kinase levels, completely reverting Sph depletion. The present data indicate for the first time that the inhibition of AGEs is effective in the maintenance of the sphingolipid rheostat through the modulation of the involved enzymes, and this could contribute to the prevention of insulin resistance observed in the pyridoxamine-treated HFD mice. Therefore, it could be of relevance to further investigate on the predictive value of Cer/Sph/S1P imbalance for insulin resistance onset and on pyridoxamine supplementation as preventive strategy.

PENGAMATAN AKTIVITAS TIKUS WISTAR JANTAN DENGAN ALAT OPTO- VARIMEX® PADA KONDISI DIET TINGGI MINYAK TRANS

ABSTRACT
 Excessive energi consumption of high-fat diet increase the body’s adipose tissue which also affect the increased secretion of leptin, this is because leptin secretion is proportional to the mass of adipose tissue. Leptin activates the lateral hypothalamus as the center of hunger and feeding activity. The aim of this study was to obtain the relationship between high trans fat feeding on male Wistar rats and feeding activity. This method uses male Wistar rats (Rattus norvegicus) as an animal model. Sixteen rats were grouped into 4 treatment groups. The first group was given additional feed 20% trans fat, the second group was given additional feed 40% trans fat, a third group was given additional feed 60% trans fat, and the last as a control group. This feed was given once a day for 70 days. Observation of activities perform using Opto-varimex® auto-track system ver. 4.31. The results showed an corellation between activity based doses of trans fat.
 Keywords: Opto-varimex®, Trans fat, Activity, Wistar rat.

Cross-generational trans fat intake facilitates mania-like behavior: Oxidative and molecular markers in brain cortex

Since that fast food consumption have raised concerns about people’s health, we evaluated the influence of trans fat consumption on behavioral, biochemical and molecular changes in the brain-cortex of second generation rats exposed to a model of mania. Two successive generations of female rats were supplemented with soybean oil (SO, rich in n-6 FA, control group), fish oil (FO, rich in n-3 FA) and hydrogenated vegetable fat (HVF, rich in trans FA) from pregnancy, lactation to adulthood, when male rats from 2nd generation received amphetamine (AMPH-4mg/kg-i.p., once a day, for 14days) treatment. AMPH increased locomotor index in all animals, which was higher in the HVF group. While the FO group showed increased n-3 polyunsaturated fatty acid (PUFA) incorporation and reduced n-6/n-3 PUFA ratio, HVF allowed trans fatty acid (TFA) incorporation and increased n-6/n-3 PUFA ratio in the brain-cortex. In fact, the FO group showed minor AMPH-induced hyperactivity, decreased reactive species (RS) generation per se, causing no changes in protein carbonyl (PC) levels and dopamine transporter (DAT). FO supplementation showed molecular changes, since proBDNF was increased per se and reduced by AMPH, decreasing the brain-derived neurotrophic factor (BDNF) level following drug treatment. Conversely, HVF was related to increased hyperactivity, higher PC level per se and higher AMPH-induced PC level, reflecting on DAT, whose levels were decreased per se as well as in AMPH-treated groups. In addition, while HVF increased BDNF-mRNA per se, AMPH reduced this value, acting on BDNF, whose level was lower in the same AMPH-treated experimental group. ProBDNF level was influenced by HVF supplementation, but it was not sufficient to modify BDNF level. These findings reinforce that prolonged consumption of trans fat allows TFA incorporation in the cortex, facilitating hyperactive behavior, oxidative damages and molecular changes. Our study is a warning about cross-generational consumption of processed food, since high trans fat may facilitate the development of neuropsychiatric conditions, including bipolar disorder (BD).

S-nitroso-N-acetylcysteine attenuates liver fibrosis in experimental nonalcoholic steatohepatitis

S-Nitroso-N-acetylcysteine (SNAC) is a water soluble primary S-nitrosothiol capable of transferring and releasing nitric oxide and inducing several biochemical activities, including modulation of hepatic stellate cell activation. In this study, we evaluated the antifibrotic activity of SNAC in an animal model of nonalcoholic steatohepatitis (NASH) induced in Sprague-Dawley rats fed with a choline-deficient, high trans fat diet and exposed to diethylnitrosamine for 8 weeks. The rats were divided into three groups: SNAC, which received oral SNAC solution daily; NASH, which received the vehicle; and control, which received standard diet and vehicle. Genes related to fibrosis (matrix metalloproteinases [MMP]-13, -9, and -2), transforming growth factor β-1 [TGFβ-1], collagen-1α, and tissue inhibitors of metalloproteinase [TIMP-1 and -2] and oxidative stress (heat-shock proteins [HSP]-60 and -90) were evaluated. SNAC led to a 34.4% reduction in the collagen occupied area associated with upregulation of MMP-13 and -9 and downregulation of HSP-60, TIMP-2, TGFβ-1, and collagen-1α. These results indicate that oral SNAC administration may represent a potential antifibrotic treatment for NASH.

The Relation between Erythrocyte Trans Fat and Triglyceride, VLDL- and HDL-Cholesterol Concentrations Depends on Polyunsaturated Fat

BackgroundTrans fatty acids (TFA) lower HDL and increase triglyceride concentrations while polyunsaturated fatty acids (PUFA) lower triglycerides and may decrease HDL concentrations. The effect of the interaction between trans fat and PUFA on lipids is uncertain.MethodsMen and women (n = 1032) in the Genetics of Lipid-Lowering Drugs and Diet Network (GOLDN) study were included. Fatty acids in erythrocyte membranes were measured with gas chromatography while data on potential confounders were obtained from questionnaires. To test the interaction between total erythrocyte PUFA (ePUFA) and TFA (eTFA) on lipid concentrations we distributed eTFA into tertiles and dichotomized ePUFA at the median concentration.ResultsFor the 1st, 2nd and 3rd tertiles of eTFA, multivariate-adjusted means±s.e.m for HDL were 46.2±1.1, 46.3±1.1 and 45.5±1.0 mg/dL among those with low ePUFA, respectively, while they were 50.0±1.1, 46.9±1.1 and 44.7±1.1 mg/dL among those with high ePUFA, respectively (P for interaction = 0.01). For the 1st, 2nd and 3rd tertiles of eTFA, multivariate-adjusted means±s.e.m for triglycerides were 178.6±11.3, 144.7±10.9 and 140.8±10.6, respectively, among those with low ePUFA, while they were 133.8±11.3, 145.7±10.9 and 149.3±11.5, respectively, among those with high ePUFA (P for interaction = 0.005). Results for VLDL were similar to those for triglycerides. No significant interactions were observed for LDL or total cholesterol.ConclusionsThe relation between trans fat and HDL, VLDL and triglycerides may depend on PUFA. The benefit of avoiding trans fat may be greater among individuals with higher PUFA intake. Supplementation with PUFA among individuals with relatively high trans fat intake may have limited benefits on lipid profiles.

Nutrient biomarker patterns and rates of cognitive decline in dementia‐free elders

BackgroundWe previously identified three distinct nutrient biomarker patterns associated with both psychometric and neuroimaging indices in a cross‐sectional analysis. The objective of this study was to examine the relationship between the nutrient biomarker patterns and cognitive decline over 2 years.MethodsThirty biological markers of diet were assayed in plasma drawn from 104 dementia‐free participants of the Oregon Brain Aging Study. Principal component analysis constructed 8 distinct nutrient biomarker patterns. A linear regression model was used to examine the change in the Clinical Dementia Rating ‐ sum of box score over two years explained by the nutrient biomarker patterns.ResultsMean age was 87±10, 62% are female, and 10% are carrying the ApoEe4 allele. Two distinct nutrient biomarker patterns were associated with rates of cognitive decline: a vitamin pattern high in vitamins B, C, E and D (p=0.001) and a high trans fat pattern (p<0.001) associated with less and more decline over two years, respectively. These findings were independent of age, gender, education years, ApoEe4 carrier status and vascular risk factors.ConclusionA nutrient biomarker pattern high in specific vitamins and low in trans‐fat may offer a prudent profile for promoting cognitive health in older populations. Funding: NIH/NCCAM AT004777 (GLB), NIH/NIA AG08017 (JAK), NIH/NCRR UL1 RR024140Grant Funding Source : NIH AT004777