Highest Total Phenolic Content Values Research Articles

PHOTOREGULATION OF THE BIOSYNTHETIC ACTIVITY OF LARICIFOMES OFFICINALIS USING COLLOIDAL SOLUTIONS OF METAL NANOPARTICLES AND LASER IRRADIATION

The aim of the work was to study the influence of biogenic metal nanoparticles on the growth characteristics and biosynthetic activity of the fungus Laricifomes officinalis, as well as the effects of photocatalytic activity of NPs after exposure to low-intensity laser radiation under deep cultivation conditions. Material and Methods. Traditional mycological methods, colloidal solutions of nanoparticles biogenic metals, and unique photobiological methods were used. Results. Treatment of the inoculum with colloidal solutions of nanoparticles of all used metals ncreased the growth of L. officinalis by 31–54%, while irradiation of the fungal inoculum with laser light in a medium with nanoparticles reduced the growth activity of the L. officinalis mycelium by 14.4–22.6%. All nanoparticles suppressed the biosynthesis of extracellular polysaccharides, whereas treatment of the inoculum with colloidal solutions of FeNPs and MgNPs stimulated the synthesis of endopolysaccharides. At the same time, laser light irradiation in the presence of AgNPs increased the amount of endopolysaccharides, while FeNPs and MgNPs slightly inhibited their synthesis. Treatment of the inoculum with colloidal metal solutions and laser light affected the total phenolic content (TPC) in the mycelial mass. The highest TPC values in ethanol extracts with AgNPs and laser light irradiation were 97.31Ѓ}3.7 mg of GAEs/g of dry mass. Conclusions. The research results gave ground to consider nanoparticles of biogenic metals (AgNPs, FeNPs, MgNPs) and low-intensity laser light as a promising regulators of the biosynthetic activity of L. officinalis in the biotechnology of its cultivation.

Mechanism-based antioxidant activity of Rubiaceae species collected from Ilocos Norte, Philippines

The Rubiaceae family, comprising 550 species in the Philippines, is a significant source of bioactive components with ethnopharmacological uses. This study assessed the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity of six Rubiaceae species, collected in Ilocos Norte, Philippines: Guettardella microphylla (Bartl. ex DC.) Merr., Timonius ternifolius (Bartl. ex DC.) Fern.-Vill., Kanapia monstrosa (A. Rich.) Arriola & Alejandro, Pyrostria triflora Arriola, Calaramo & Alejandro, Pyrostria subsessilifolia (Merr.) Arriola & Alejandro, and Psychotria luzoniensis (Cham. & Schltdl.) Fern.-Vill. Identification of the plant species was done using morphological characterization. The TFC results ranged between 1.86-3.81 mg quercetin equivalent/g dry weight (GAE/g DW), while TPC indicated 5.47-17.17 mg gallic acid equivalent/g dry weight (QE/g DW). G. microphylla showed the highest TFC (3.81±0.20 mg QE/g DW) while P. triflora exhibited the highest TPC (17.17±0.83 mg GAE/g DW). Antioxidant profiling showed P. luzoniensis exhibiting the highest activity in the NOS, ABTS, DPPH, and FRAP assays. G. microphylla showed the highest hydrogen peroxide scavenging activity, while T. ternifolius demonstrated the highest hydroxyl radical scavenging activity. Findings suggest that the crude methanolic extracts of the Rubiaceae species have relatively high TPC and TFC values and exhibit promising antioxidant capacities.

Effects of pretreatment and drying temperatures on physicochemical and antioxidant properties of dried duku (Lansium domesticum)

Duku (Lansium domesticum) is a native of Southeast Asia. Duku is a seasonal fruit and highly deteriorated due to high moisture content of more than 80 %, thus difficult to commercialise due to the short shelf life. The transformation of fresh into dried fruit will increase the commercial values as well as be a solution to reduce fruit loss. This study was conducted to evaluate the effects of pretreatments and drying temperatures on physicochemical and antioxidant properties in dried duku fruit. The duku fruits (whole, flesh, peels and seeds) were subjected to pretreatment process with ascorbic acid solution, sodium metabisulfite solution, sodium erythorbate solution and blanching. Then, the samples were dried at 50 °C, 60 °C, 70 °C and 80 °C, respectively. Determination of total phenolic content (TPC) was carried out based on the Folin-Ciocalteau method. The antioxidant activities were evaluated using ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl). The pH values of all parts of duku were decreased as the drying temperature increases. Seed (pretreated with sodium metabisulfite, dried at 80 °C) showed the highest total phenolic content value with 116.84 ± 0.31 mg gallic acid equivalents per g fruit extract (GAE/g), while lowest in flesh (pretreated with ascorbic acid, dried at 50 °C). Seed (pretreated with sodium metabisulfite, dried at 50 °C) has the highest antioxidant capacity with IC50 value of 41.15 µg/ml (ABTS free radical scavenging) and 121.35 µg/ml (DPPH free radical scavenging) compared to other fruit parts, pretreatments and drying temperatures. The findings of this study indicate the potential of all parts of duku fruit as a valuable source of nutrients. Information on the nutritional and antioxidant properties of this Malaysia's seasonal fruit reveals its potential to be commercialized into different food and beverage products.

Effects of cooking techniques on phenolic content and sensory profiles of cauliflower

In this study, the effects of the cooking methods (frying, stir-frying, air-frying, boiling, steaming, baking and sous-vide, and microwaving) on total phenolic content (TPC) and sensory profile of cauliflower have been investigated. Higher cooking temperatures have resulted in higher TPC values, which can be attributed to the generation of new phenolics by the Maillard reaction and cleavage of bound phenolics. The highest TPC and bitterness were obtained when steaming and baking (180°C for 20 min) were applied. While the boiling procedure results in low bitterness and sweetness due to the loss of related compounds in boiling water, the taste of cooked cauliflower was higher when steamed (12 min) and stir-fried (4.5 min). Overall acceptability of the boiled, steamed, and fried samples was higher than in baked and sous-vide treated samples. Steaming was determined as the best technique regarding TPC content and sensory properties.

Phytochemical Assessment, Antioxidant Activity, and in vitro Wound Healing Potential of Polygonum minus Huds

The increasing prevalence of chronic wounds, associated with aging, obesity, and diabetes, is compounded by bacterial resistance and adverse effects associated with commercial wound care products. Therefore, treatment modalities to accelerate the healing process are constantly being sought. This study aimed to investigate the phytochemical composition, antioxidant, and wound healing potential of the aqueous extract derived from the stems and leaves of Polygonum minus. The extract was subjected to phytochemical evaluation to assess the diversity of secondary metabolites. The total phenolic content (TPC) and total flavonoid content (TFC) were measured using the Folin-Ciocalteu colorimetric and aluminium chloride methods, respectively. The antioxidant activity was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and ferric-reducing antioxidant power (FRAP) assays. The wound healing effects were evaluated using proliferation and migration assays on human epidermal keratinocyte (HaCaT) cells. The phytochemical evaluation of the aqueous extract revealed the presence of flavonoids, terpenoids, alkaloids, saponins, tannins, steroids, and cardiac glycosides. Furthermore, the extract exhibited high TPC (137.74 ± 0.75 µg/mL GAE) and TFC (177.08 ± 3.16 µg/mL QE) values, as well as radical scavenging activity at 79.50% in the DPPH assay and a FRAP value of 1485.67 ± 0.05 µM/g Fe2+. At the lowest concentration of 7.81 μg/mL, the extract significantly stimulated cell proliferation and migration within 24 hours of treatment. The stimulation of cell migration was comparable with that of allantoin, which was used as a positive control. This study indicated that the P. minus aqueous extract contains a high concentration of phenolic compounds, which could contribute significantly to its antioxidant activity and promote the proliferation and migration of keratinocytes. The findings suggest that the extract may merit further investigation for potential applications in topical therapy related to wound healing.

The Efficiency of Selected Green Solvents and Parameters for Polyphenol Extraction from Chokeberry (Aronia melanocarpa (Michx)) Pomace.

Chokeberry pomace is a potential source of natural colourants, antimicrobial agents, and anti-senescence compounds due to its high polyphenols content. Therefore, this study assessed the efficiency of green solvents (50% ethanol, 50% glycerol, and 100% distilled water, all acidified with 1% citric acid or 1% formic acid) for anthocyanin and total phenolic content (TPC) extraction from lyophilised chokeberry pomace. Extraction was performed in a water bath at 40, 50, and 60 °C for 60 and 120 min, followed by ultrasonic treatment for 15 and 30 min. Based on the results, 50% ethanol + 1% citric acid yielded significantly higher total anthocyanin content in the case of both spectrometric and HPLC measurements (1783 ± 153 mg CGE/100 g DW and 879.5 mg/100 g DW) at 50 °C for 60 min. Citric acids seem more effective compared to formic acid. The highest values of TPC were obtained with 50% glycerol + 1% formic acid at 50 °C for 60 min (12,309 ± 759 mg GAE/100 g DW). This study provides evidence that a substantial quantity of polyphenols, which can potentially be used as a natural food additive, can be efficiently extracted with 50% ethanol + 1% citric acid or 50% glycerol at 50 °C for 60 min from chokeberry pomace.

The anti-allergic potential of stingless bee honey from different botanical sources via modulation of mast cell degranulation

BackgroundAllergy is an inflammatory disorder affecting around 20% of the global population. The adverse effects of current conventional treatments give rise to the increased popularity of using natural food products as complementary and alternative medicine against allergic diseases. Stingless bee honey, commonly known as Kelulut honey (KH) in Malaysia, has been used locally as a traditional remedy to relieve cough and asthma. This study evaluated the anti-allergic potential of KH collected from four different botanical sources on phorbol ester 12-myristate-3-acetate and calcium ionophore-activated human mast cells.MethodsThe present study examined the inhibitory effects of all collected honey on the release of selected inflammatory mediators, such as tumor necrosis factor-α (TNF-α), interleukin (IL)-4, IL-6, IL-8, histamine, and β-hexosaminidase in an activated HMC. Besides that, all honey's total phenolic content (TPC) was also examined, followed by using liquid chromatography with tandem mass spectrometry (LC–MS/MS) to identify the phytochemicals in the honey. Further examination of the identified phytochemicals on their potential interaction with selected signaling molecules in an activated mast cell was conducted using computational methods.ResultsThe results indicated that there were significant inhibitory effects on all selected inflammatory mediators’ release by KH sourced from bamboo (BH) and rubber tree (RH) at 0.5% and 1%, but not KH sourced from mango (AH) and noni (EH). BH and RH were found to have higher TPC values and were rich in their phytochemical profiles based on the LC–MS/MS results. Computational studies were employed to determine the possible molecular target of KH through molecular docking using HADDOCK and PRODIGY web servers.ConclusionsIn short, the results indicated that KH possesses anti-allergic effects towards an activated HMC, possibly by targeting downstream MAPKs. However, their anti-allergic effects may vary according to their botanical sources. Nevertheless, the present study has provided insight into the potential application of stingless bee honey as a complementary and alternative medicine to treat various allergic diseases.

Effect of controlled enzymatic treatment on the physicochemical, structural and functional properties of high-intensity ultrasound treated album (Chenopodium album) protein

The present study was carried out to examine the effect of enzymatic treatment on high-intensity ultrasound modified album proteins. The results showed that enzymatic treatment induced structural changes which in turn improved physiochemical and functional characteristics of album proteins. Trypsin treated album proteins showed highest degree of hydrolysis values of 18.99%. Enzymatic treatment decreased particle size, poly-dispersibility index, zeta-potential, turbidity, total SH content and SS-bonds, however, DPPH radical scavenging activity, total phenolic content, solubility, surface hydrophobicity, free SH groups increased significantly (p ≤ 0.05). Smallest particle size reduction of 400 nm was observed in the case of pepsin treated album proteins. FTIR resulted showed major peak shifting in the wavelength ranging from 1600 to 1700 cm−1 in enzymatically treated album proteins indicating structural and conformational changes. Enzymatic treatment decreased molecular weight of album proteins as indicated by SDS-PAGE results. X-ray diffraction analysis showed decline in crystallinity of all the enzymatically treated album proteins and highest decline was observed in protease treated album proteins. FE-SEM results showed the appearance of cracks and porous type surfaces in all the enzymatically treated album proteins, however, the control samples showed somewhat smooth surface morphologies. Thermal characteristics, particularly denaturation temperature showed significant increase in enzymatic treated album proteins. These structural and conformational changes reduced particle size, improved color characteristics, flow properties, antioxidant activity and total phenolic content of enzymatic treated album proteins. For instance, trypsin treated album proteins showed highest antioxidant activity and highest total phenolic content values of 66.75% and 8.61 (mg GAE/g), respectively. Pepsin treated album proteins showed lowest turbidity values of 0.73 and trypsin treated album proteins showed highest solubility values of 99.73%. Loose bulk density, packed bulk density and true density of enzymatic treated album proteins was found significantly higher than those of control album proteins, whereas, Hausner's ratio, Carr's index and Tan θ was found significantly higher in trypsin treated album proteins. Enzymatic treatment coupled with HIUS modification favorably impacted the structural, physiochemical and functional properties of album proteins.

Effects of fermentation on the quality characteristics and biological activities of citrus juices

In this study, physicochemical, microbiological qualities and biological activities of fruit juices obtained from three different citrus fruits and fermented products obtained as a result of inoculation of nine different lactic acid bacteria were investigated. As a result of the research, was determined that titration acidity, free radical scavenging activity and total phenolic substance values increased due to fermentation process, whereas pH, aw, dry matter, viscosity, brix and L* values decreased. Among the samples, the lowest DPPH value was determined by B. animalis spp.lactis inoculation, and the highest total phenolic content value was found in the samples obtained by L. plantarum inoculation among three citrus species. Moreover, it was determined that the antibacterial and antifungal effects of the obtained products increased on the strains used in the study, especially with fermentation. It was determined that the antibacterial effect is more effective on Gram (-) bacteria in general.

Antimicrobial Potential, Antioxidant Activity, and Phenolic Content of Grape Seed Extracts from Four Grape Varieties

The aim of this study was to evaluate the total phenolic content, composition, and antioxidant and antibacterial activities of four grape seed extracts (Cabernet Sauvignon, Marselan, Pinot Noir, and Tamyanka). The total phenolic content (TPC) and flavonoid, anthocyanin, procyanidin, ascorbic acid, DPPH, and ABTS antioxidant capacities of the grape seed extracts (GSEs) were determined. The extracts showed high TPC values (79.06-111.22 mg GAE/g). The individual components in the GSEs were determined using HPLC. High contents of catechin, epicatechin, and procyanidin B1 were found in the extracts. The antimicrobial activity of the obtained GSEs against Staphylococcus aureus, Bacillus cereus, and Escherichia coli was evaluated using the agar diffusion test and a test to determine the minimum inhibitory concentration (MIC). According to the effect on the growth of pathogens, the extracts were ranked in the following order: Pinot Noir > Marselan > Cabernet Sauvignon > Tamyanka. The tested bacteria showed high sensitivity to the extracts (MIC = 0.12-0.50 mg/mL). According to the MIC values, the bacteria were in the following order: S. aureus > B. cereus > E. coli. A correlation was found between the phenolic content of the GSEs and their antibacterial potential. The obtained results show that the studied GSEs have good potential as antioxidant and antimicrobial agents.

Subcritical Water Extraction as an Effective Technique for the Isolation of Phenolic Compounds of Achillea Species

The genus Achillea has significant medical potential due to the presence of highly bioactive compounds in its chemical composition. To take advantage of plants’ biomedical potential, it is of great importance to use a proper extraction process. This study aimed to determine and compare the preliminary chemical composition of five different Achillea species extracted with two conventional (infusion and maceration) and two non-conventional (ultrasound-assisted extraction (UAE) and Subcritical water extraction (SWE)) techniques. The extracts were prepared using the previously described procedures for infusion, maceration, UAE and SWE extraction. For all extracts, the extraction yield (dry extract (DE)) was determined. The analyzed extracts were preliminarily chemically characterized spectrophotometrically in terms of total phenolic content (TPC) and total flavonoid content (TFC). The obtained results showed that non-conventional techniques delivered higher values of TPC and TFC than conventional. There is a statistically significant increase in DE and TPC content when applying SWE for all observed Achillea species. The highest DE value, 48.80 ± 1.76%, was observed for A. asplenifolia. The highest TPC values were observed after applying SWE: 93.63 ± 1.01 mg GAE/g DE for A. millefolium, and 90.12 ± 0.87 mg GAE/g DE for A. crithmifolia. The results for TFC revealed a statistically significant difference in values, with A. nobilis subsp. nelreichii as the sample with the highest content of TFC (11.11 ± 0.22 mg QE/g DE) when using UAE. Consequently, it could be concluded that SWE is a superior non-conventional extraction technique, and A. nobilis subsp. nerleichii presents as the most promising plant.

노지 및 인공광 식물공장에서 생산된 삼채 잎의 항산화 및 항염 효과 비교

This study was conducted to evaluate and compare the antioxidant and anti-inflammatory effects of Allium hookeri grown in an outfield and those grown in a plant factory using different lights (FL, fluorescent light; LED-R, red light-emitting diode (LED); LED-B, blue LED; LED-R+B, red+blue LED). The antioxidant effects were determined by measuring the total phenolic content (TPC), DPPH and ABTS radical scavenging activities, and superoxide dismutase (SOD) and catalase (CAT) activities. The anti-inflammatory effects were determined by measuring the inhibition of nitric oxide (NO) production. The results showed that extracts of the leaves of A. hookeri grown in the outfield had the highest TPC value. The A. hookeri leaves grown in the plant factory showed higher antioxidant effects compared to those grown in the outfield as evaluated by the DPPH and ABTS radical scavenging activities and SOD activity. The highest CAT activity was found in the extract of leaves exposed to LED-R+B lights. The viability of RAW264.7 cells cultured with all the extracts of A. hookeri leaves was over 100% compared to that of the media control, and there was no cytotoxicity observed in any of the extracts. The NO production was significantly reduced in all the groups and the inhibitory effects of the A. hookeri leaves were dose-dependent. These results showed that the antioxidant and anti-inflammatory effects of A. hookeri grown in the plant factory using artificial lights are as good as those of the same plants grown in the outfield. Thus, artificial lights may be used to improve the functional characteristics of A. hookeri.

Valorization of fruit waste from Cucurbitaceae family: Profiling of phytoconstituent of Benincasa hispida and Citrullus lanatus rinds using ultrasound-assisted extraction

Food agricultural waste from fruit products such as rinds can be one source of environmental pollution. Therefore, the study was conducted to investigate the total phenolic content, total flavonoid content, antioxidant property, and chemical profiling using gas chromatography-mass spectrometer (GC-MS) and high-performance liquid chromatography tandem mass spectrometry of triple quadrupole (HP LCMS-MS QTOF). Ultrasound-assisted extraction for 45 min was used as a green technique to extract bioactive compounds from watermelon (Citrullus lanatus) and wintermelon (Benincasa hispida) rinds. The total phenolic content and total flavonoid content of ultrasound-assisted extraction were compared with maceration technique. As revealed by the results, maceration technique had a lower total phenolic content for both C. lanatus rind (7.07 ± 0.86 μg GAE/mg) and B. hispida rind (13.87 ± 0.11 μg GAE/mg) compared to ultrasound-assisted extraction. The similar trends can be observed in total flavonoid content of C. lanatus rind (4.97 ± 0.29 μg RE/g) and B. hispida rind (8.30 ± 0.17 μg RE/g) by maceration which showed a lower quantity compared to ultrasound-assisted extraction. The ultrasound-assisted extraction was selected for further analysis. The total phenolic content assay found that the B. hispida rind extract exhibited higher total phenolic content values (23.39 ± 0.22 μg GAE/mg), than C. lanatus rind extract (15.10 ± 0.58 μg GAE/mg). B. hispida rind extract had a higher total flavonoid content (11.17 ± 0.17 μg RE/g) than C. lanatus rind extract (6.16 ± 0.09 μg RE/g). The antioxidant activity was investigated using DPPH scavenging assay. The antioxidant capacity of B. hispida rind extract (20872.19 ± 1301.47 mg/mL) stronger compared to C. lanatus rind extract (23514.47 ± 678.70 mg/mL). Results revealed that there was a negative correlation between total phenolic content, total flavonoid content, and DPPH scavenging activity IC50. The phytoconstituent determination in B. hispida and C. lanatus rind extract showed both contained phenolic, malic acid, and other compounds that might have contributed to antioxidant activities. Phenolic, flavonoid, and antioxidant activities are useful to valorize B. hispida and C. lanatus rinds as they can be converted into new income-generating resources in agriculture and the food industry.

Phenolic and flavonoid content with agro morphological characters of 12 accessions of Justica gendarussa grew in Indonesia

Abstract. Marliani N, Artika IM, Rafi M, Syukur M, Galingging RY, Nurcholis W. 2022. Phenolic and flavonoid content with agro morphological characters of 12 accessions of Justica gendarussa grew in Indonesia. Biodiversitas 23: 5101-5107. Justicia gendarussa Burm. f is a medicinal plant used in traditional and modern medicine. This study was conducted to determine the agro morphological characters, phenolic and flavonoid content of 12 accessions of J. gendarussa plants obtained from various locations in Indonesia and then grown in the same conditions and places. The agro morphological characteristics include plant height, number of leaves, number of branches, wet weight, and dry weight of leaves and stems. Total phenolic content (TPC) and total flavonoid content (TFC) were analyzed by spectrophotometric method. The results showed significant differences in the characters of 12 accessions of J. gendarussa (p<0.05). Accessions of BK4 (Bekasi 4) and KP (Kejayan Pasuruan) had the best agro morphological characteristics. Accessions of BK4 (Bekasi 4) and BG (Bogor) had the best growth rates. The highest TPC value was obtained from TG (Trenggalek) accession (0.752 mg GAE/g DW) and the highest TFC (1.899 mg QE/g DW from LK (Langkat) accession. The best accessions based on agro morphological characters were BK4 (Bekasi 4) and KP (Kejayan Pasuruan), and based on the content of polyphenols were TG (Trenggalek) and LK (Langkat). The accessions of BK4, BG, TG, and LK could be further developed into superior varieties of J. gendarussa.

Drying date plum (Diospyros lotus L.) fruit: Assessing rehydration properties, antioxidant activity, and phenolic compounds.

Date plum (Diospyrus lotus L.) is an edible fruit from the Ebenaceae family, rich in nutrients, and having tremendous medicinal properties. This paper attempted to show the influence of different parameters of convective drying such as temperature (50, 60, 70, and 80°C) and air velocity (0.5, 1.0, and 1.5m/s) on the shrinkage and microstructure, rehydration properties, antioxidant activity, and phenolic compounds of date plum. The drying caused significant changes in the color, actual size, and distribution of the fruit cells of date plum. The total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) of fresh date plum were 0.81±0.00mg GAE/g, 0.23±0.10mg ECE/g, 7.15±1.09mmol ISE/g, and 14.92±0.88mmol/TE, respectively. The drying at 70°C had the highest values of TPC, TFC, gallic acid, chlorogenic and syringic acids, catechin, quercetin-3-glucoside, resveratrol, and DPPH. The drying air velocities showed no significant effects on the antioxidant contents and the antioxidant activity. Of the models applied to the drying kinetics, the Midilli model was found as the best model to describe the drying kinetics of date plum. In addition, the Weibull model was found as the most successful among the models applied to the rehydration kinetics of date plum. According to the achieved findings, the convective drying temperature of 70°C is the optimum temperature to produce the dehydrated date plum. Practical Application This work has revealed the drying conditions responsible for preserving the phenolic compounds, total flavonoid content, and antioxidant features of D. lotus L. The study found the optimum drying conditions, and Midilli and Weibull models were the most fitted models to describe the drying and rehydration behaviors of D. lotus L. fruits, respectively. The drying provides a reasonable value of the possibility of continuous consumption of the fruits dried afforded on off-seasons. The dried fruits are widely used for multipurpose and have been extensively used in food industries due to their rich nutraceutical and antioxidant compounds.

Phytochemical Screening, and In Vitro Evaluation of the Antioxidant and Dermocosmetic Activities of Four Moroccan Plants: Halimium antiatlanticum, Adenocarpus artemisiifolius, Pistacia lentiscus and Leonotis nepetifolia

In this study, four Moroccan plants, Halimium antiatlanticum, Adenocarpus artemisiifolius, Pistacia lentiscus and Leonotis nepetifolia, were evaluated for their phytoconstituents and biological activities. Methanolic extracts of these plants were obtained by Soxhlet apparatus, phytochemical screening was performed, and the total phenolic and flavonoid contents were determined. Then, the antioxidant and dermocosmetic activities of the methanolic extracts were evaluated. The obtained results revealed that the leaves and/or aerial parts contained tannins, polyphenols, flavonoids, coumarins, carotenoids, terpenoids and saponins. The higher total phenolic content values were recorded on Pistacia lentiscus and Halimium antiatlanticum with 396.64 ± 30.79 and 304.96 ± 55.61 mgGAE/gDW, respectively. The antioxidant activity was measured by DPPH, ABTS and FRAP assays, and showed that Pistacia lentiscus and Halimium antiatlanticum were the most active extracts, with, respectively, IC50 values of 3.705 ± 0.445 and 5.037 ± 0.122 µg/mL for DPPH. The same results were observed for the FRAP and ABTS assays. Those extracts also showed a strong collagenase inhibitory activity at 200 µg/mL, with 78.51 ± 2.27% for Pistacia lentiscus and 73.10 ± 8.52% for Halimium antiatlanticum. Adenocarpus artemisiifolius showed the highest elastase inhibition rate, with 76.30 ± 5.29%. This study disclosed the dermocosmetic potential of Halimium antiatlanticum and Adenocarpus artemisiifolius, two Moroccan endemic plants that can be traditionally used by local populations or exploited by the cosmetic industry.

Dwarf Kiwi (Actinidia arguta Miq.), a Source of Antioxidants for a Healthy and Sustainable Diet

The feasibility of using dwarf kiwi fruits (Actinia arguta Miq.) as a healthy and sustainable food, compared to other types of commercial kiwi fruits, was evaluated in the present study. The overall antioxidant capacity of these fruits was assessed by either extraction-dependent methods (ABTS, ORAC) or the direct method called Quick, Easy, New, CHEap, Reproducible (QUENCHER) (DPPH, FRAP, Folin–Ciocalteu), applied for the first time to analyze kiwi fruits. With this methodology, all the molecules with antioxidant capacity are measured together in a single step, even those with high molecular weight or poor solubility in aqueous extraction systems, such as antioxidant dietary fiber. The effect of kiwi extracts on physiological and induced intracellular reactive oxygen species (ROS) production on IEC-6 cells was also analyzed, as well as total phenolic content (TPC) by Fast Blue BB, flavonols, hydroxycinnamic acids, and hydroxybenzoic acids. A. arguta fruits showed the highest values in all the antioxidant assays, being remarkably higher than the other kiwi species for Q-FRAP and Q-DPPH. Dwarf kiwi showed the highest potential in reducing physiological ROS and the highest values of TPC (54.57 mgGAE/g), being hydroxybenzoic acids the main phenolic family found (2.40 mgGAE/g). Therefore, dwarf kiwi fruits are a natural source of antioxidants compared to conventional kiwi fruits, being a sustainable and healthy alternative to diversify fruits in the diet.

Extraction of Bioactive Metabolites from Achillea millefolium L. with Choline Chloride Based Natural Deep Eutectic Solvents: A Study of the Antioxidant and Antimicrobial Activity

In this study, the extraction efficiency of natural deep eutectic solvents (NADES) based on choline chloride as a hydrogen bond acceptor (HBA) and five different hydrogen bond donors (HBD; lactic acid, 1,4-butanediol, 1,2-propanediol, fructose and urea) was evaluated for the first time for the isolation of valuable bioactive compounds from Achillea millefolium L. The phytochemical profiles of NADES extracts obtained after ultrasound-assisted extraction were evaluated both spectrophotometrically (total phenolic content (TPC) and antioxidant assays) and chromatographically (UHPLC-MS and HPLC-UV). The results were compared with those obtained with 80% ethanol, 80% methanol, and water. The highest TPC value was found in the lactic acid-based NADES (ChCl-LA), which correlated with the highest antioxidant activity determined by the FRAP analysis. On the other hand, the highest antiradical potential against ABTS+• was determined for urea-based NADES. Phenolic acids (chlorogenic acid and dicaffeoylquinic acid isomers), flavones (luteolin and apigenin), and their corresponding glucosides were determined as the dominant individual phenolic compounds in all extracts. The antibacterial and antifungal properties of the extracts obtained against four bacterial cultures and two yeasts were evaluated using two methods: the agar dilution method to obtain the minimum inhibitory concentration (MIC) and the minimum bactericidal or fungicidal concentration (MBC or MFC), and the disc diffusion method. ChCl-LA had the lowest MIC and MBC/MFC with respect to all microorganisms, with an MIC ranging from 0.05 mg mL−1 to 0.8 mg mL−1, while the water extract had the weakest inhibitory activity with MIC and MBC/MFC higher than 3.2 mg mL−1.

In vitro digestion effect on CCK and GLP-1 release and antioxidant capacity of some plant-based milk substitutes.

Recently, plant-based milk substitutes, as an emerging industry, are receiving more attention. Despite that, these dairy alternatives have not been adequately studied for their functional properties. Thus, the current research was devoted to study the satiety potential through in vitro secretion of cholecystokinin (CCK) and glucagon-like peptide-1 (GLP-1), and the antioxidant capacity of these dairy alternatives after in vitro digestion. The enteroendocrine cell line, STC-1, was used to measure satiety hormones release (CCK and GLP-1) by enzyme-linked immunoassay (ELISA). Also, total phenolic and flavonoid contents and antioxidant capacity (using oxygen radical absorbance capacity [ORAC], ferric reducing antioxidant power [FRAP], and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid [ABTS] assays) were measured before and after in vitro digestion. The results demonstrated that CCK secretion was significantly (p<0.05) higher for cow's milk (350.64 pg ml-1 ) as compared to plant-based milk substitutes. Among the plant-based milk substitutes, tiger nut milk showed the highest CCK stimulant (228.96 pg ml-1 ), followed by hazelnut milk (220.04 pg ml-1 ). Concerning GLP-1 release, the data exhibited that spelt milk was the food with the highest induction of GLP-1 hormone secretion, followed by cow's milk (910.17 and 876.59 pg ml-1 , respectively), but without any significant differences between them. total phenolic content (TPC) values strongly increased after in vitro digestion, cow's milk and soymilk being the samples with the highest TPC values after in vitro digestion (165.76 and 153.71mg GAE/100ml, respectively). In line with TPC values, soymilk had the highest ORAC, ABTS, and FRAP values after in vitro digestion (25.41, 8.17, and 2.51µmol TE/ml, respectively). Thus, these dairy alternatives could be an adequate substitute for cow's milk, according to its satiety and antioxidant capacity. PRACTICAL APPLICATION: The first study deals with the efficacy of plant-based milk substitutes as satiating foods. Spelt milk was the highest stimulant for GLP-1 secretion. Tiger nut milk showed the highest CCK stimulant, followed by hazelnut milk. TPC and total antioxidant capacity increased after digestion.

ANTIOXIDANT CAPABILITIES OF Litsea garciae BARK EXTRACTS AND THEIR RELATION TO THE PHYTOCHEMICAL COMPOSITIONS

The plant species belonging to the Litsea genus are widely investigated due to their nutritional and medicinal purposes. In this regard, this study is another similar sincere effort in which the antioxidant property and phytochemical composition of Litsea garciae (L. garciae) bark’s hexane, chloroform, methanol, and aqueous extracts were evaluated to confirm its traditional benefits. The total flavonoid content (TFC) and total phenolic content (TPC) were determined first, followed by an assessment of in vitro antioxidant activity using the DPPH and FRAP assays. The composition of the secondary metabolites was determined using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry (UHPLC-MS). As a result, methanol extract was recorded to have the highest TPC value aligned with its positive appearance in phytochemical screening. Its antioxidant capacity indicated the least IC50. The results indicated that the significant free radical scavenging activity was due to the methanolic extract's high phenolic content. The secondary metabolites found in the methanol extract varied significantly according to UHPLC-MS analysis. The major phenolic compounds were found including N-trans-feruloyl-4-O-methyldopamine, N-cis-feruloyltyramine, epicatechin-(4beta-&gt;6)-epicatechin-(2beta-&gt;7,4beta-&gt;8)-epicatechin, 7-Hydroxy-3-(4-methoxyphenyl)-4-propyl-2H-1-benzopyran-2-one and 9-O-Methylneodunol. In general, the results indicate that L. garciae bark may be a promising source of novel natural compounds with antioxidative properties.