High Species Diversity Research Articles

Butterfly Abundance and Diversity in Different Habitat Types in the Usangu Area, Ruaha National Park

Insects are key fauna species that respond quickly to disturbances and environmental changes. They act as good indicators of habitat, community, or ecosystem quality. Among the great diversity of insects, butterflies stand as ideal bio-indicators for ecosystem function and are sensitive to changes in habitat composition and structure. This study was carried out to examine the diversity and abundance of butterflies across the restored habitats in Usangu area part of Ruaha National Park (RUNAPA) from May 2022 to June 2022 using the walking transect method supplemented by sweep nets and butterfly baited traps. A total of six transects of 1 km in length were laid in the four main habitat types selected in Usangu area including grassland, Miombo woodland, Vachellia/Commiphora woodland, and riverine forest. Searches were conducted in the morning and evening. The Miombo woodland and riverine forest habitats exhibited relatively higher species diversity, richness, evenness, abundance, and a higher number of habitat-restricted species, while Vachellia/Commiphora woodland and grassland habitats recorded the lowest diversity and abundance as well as the lowest number of habitat-restricted species. Family Nymphalidae was the most dominant followed by Pieridae while Papilionidae and Hesperiidae were scarce in the study area. This study clearly shows the importance of Miombo woodland and riverine forest habitats in sustaining rich butterfly diversity and abundance in Usangu area. The two habitats must be effectively managed and conserved for sustaining ecological health and integrity of Usangu area. The Usangu area’s Miombo woodland and riverine forest habitats have immense potential for butterfly tourism and they can offer an excellent opportunity to promote conservation efforts and raise public awareness. However, it is crucial to monitor these habitats closely as any environmental changes that may occur could harm the butterfly diversity and abundance in the area. Preserving this wilderness to maintain a thriving butterfly population is of utmost importance.

A case study on the application of spore sampling for the monitoring of macrofungi.

Fungi play a vital role in ecosystem functioning, yet significant knowledge gaps persist in understanding their diversity and distribution leading to uncertainties about their threat status and extinction risk. This is partly owed to the difficulty of monitoring fungi using traditional fruiting body surveys. The present study evaluates airborne environmental DNA (eDNA) sampling as a monitoring tool with a focus on grassland macrofungi. We applied active and passive air sampling methods, complemented by extensive field surveys of waxcap and clavarioid fungi-species groups of high relevance for conservation. Twenty-nine species were recorded during the field surveys, 19 of which were also detectable by ITS2 metabarcoding of the collected samples. An additional 12 species from the studied genera were identified exclusively in air eDNA. We found that the patterns of species detection and read abundance in air samples reflected the abundance and occurrence of fruiting bodies on the field. Dispersal kernels fitted for the three dominant species predicted rapidly decreasing spore concentrations with increasing distance from fruitbodies. Airborne assemblages were dominated by a high diversity of common species, while rare and threatened red-listed species were under-represented, which underscores the difficulty in detecting rare species, not only in conventional surveys. Considering the benefits and drawbacks of air sampling and fruitbody surveys, we conclude that air sampling serves as a cost- and time-efficient tool to characterize local macrofungal communities, providing the potential to facilitate and improve future fungal monitoring efforts.

Detecting kelp-forest associated metazoan biodiversity with eDNA metabarcoding

Environmental DNA (eDNA) metabarcoding is a promising tool for monitoring marine biodiversity, but remains underutilised in Africa. In this study, we evaluated the ability of aquatic eDNA metabarcoding as a tool for detecting biodiversity associated with a South African kelp forest, an ecosystem that harbours high diversity of species, many of which are endemic, but are also sensitive to changing environmental conditions and anthropogenic pressures. Using fine-scale spatial (1 m and 8 m) and temporal (every four hours for 24 h) sampling of aquatic environmental DNA and targeting two gene regions (mtDNA COI and 12S rRNA), metabarcoding detected 880 OTUs representing 75 families in the broader metazoan community with 44 OTUs representing 24 fish families. We show extensive variability in the eDNA signal across space and time and did not recover significant spatio-temporal structure in OTU richness and community assemblages. Metabarcoding detected a broad range of taxonomic groups, including arthropods, ascidians, cnidarians, echinoderms, ctenophores, molluscs, polychaetes, ichthyofauna and sponges, as well as Placozoa, previously not reported from South Africa. Fewer than 3% of OTUs could be identified to species level using available databases (COI = 19 OTUs, 12S = 11 OTUs). Our study emphasizes that kelp-forest associated biodiversity in South Africa is understudied, but that with careful consideration for sampling design in combination with increased barcoding efforts and the construction of regional databases, eDNA metabarcoding will become a powerful biomonitoring tool of kelp-forest associated biodiversity.

The loss of plant functional groups increased arthropod diversity in an alpine meadow on the Tibetan Plateau.

Plant species loss, driven by global changes and human activities, can have cascading effects on other trophic levels, such as arthropods, and alter the multitrophic structure of ecosystems. While the relationship between plant diversity and arthropod communities has been well-documented, few studies have explored the effects of species composition variation or plant functional groups. In this study, we conducted a long-term plant removal experiment to investigate the impact of plant functional group loss (specifically targeting tall grasses and sedges, as well as tall or short forbs) on arthropod diversity and their functional groups. Our findings revealed that the removal of plant functional groups resulted in increased arthropod richness, abundance and the exponential of Shannon entropy, contrary to the commonly observed positive correlation between plant diversity and consumer diversity. Furthermore, the removal of different plant groups had varying impacts on arthropod trophic levels. The removal of forbs had a more pronounced impact on herbivores compared to graminoids, but this impact did not consistently cascade to higher-trophic arthropods. Notably, the removal of short forbs had a more significant impact on predators, as evidenced by the increased richness, abundance, the exponential of Shannon entropy, inverse Simpson index and inverse Berger-Parker index of carnivores and abundance of omnivores, likely attributable to distinct underlying mechanisms. Our results highlight the importance of plant species identity in shaping arthropod communities in alpine grasslands. This study emphasizes the crucial role of high plant species diversity in controlling arthropods in natural grasslands, particularly in the context of plant diversity loss caused by global changes and human activities.

Preliminary Study on Floral Distribution, Abundance and Diversity of Mangroves in Vankalai, North-west Coast of Sri Lanka

Sri Lanka harbors a remarkable amount of mangrove habitats. Northern Province shares about 16% of the total mangrove extent of the country. Vankalai consists of several ecosystems which range from arid-zone thorn scrubland, arid-zone pastures and maritime grasslands, sand dunes, mangroves, salt marshes, lagoons, tidal flats, sea-grass beds and shallow marine areas. Due to the integrated nature of shallow wetland and terrestrial coastal habitats, this sanctuary is highly productive, supporting high ecosystem and species diversity. The research was aimed to identify the distribution, abundance and diversity of mangroves in Vankalai of the Northern Province, Sri Lanka. Ten 25m wide belt transects with 10m intervals were randomly arranged in the small patch of mangrove vegetation perpendicular to the 350m of shoreline across the water-land gradient. Each transect was divided into five 5mx5m subplots for convenience of sampling. True mangrove species in each transect were identified and counted. The quantitative data has been used to compute the Shannon diversity index (H), Simpson Index (D) and Shannon Evenness (E), which were used to compare the diversity of the study sites. Ten different mangrove species including Avicennia marina (n=115), Avicennia officinalis (n=73), Rhizophora apiculate (n=33), Rhizophora mucronata (n=102), Sonneratia alba (n=3), Pemphis acidula (n=4), Excoecaria agallocha (n=40), Lumnitzera racemose (n=91), Bruguiera cylindrical (n=97), Ceriops tagal (n=31) were identified and they were belonging to five families; Avicenniaceae (n=2), Rhizophoraceae (n=5), Lythraceae (n=1), Euphorbiaceae (n=1) and Combretaceae (n=1). Further, based on the IUCN status, two mangrove species (Sonneratia alba and Bruguiera cylindrical) were identified as endangered species. The highest Shannon diversity index (H=2.21) and the lowest Simpson index value (D=0.14) were observed in the transects of point 1 due to minimal disturbances. The least Shannon diversity index and evenness were recorded in the point 5 as 1.65 and 0.85 respectively at the point 5 since fishing and tourism activities take place in the proximity of this place. The Shannon diversity index varied at the points of 1 (H=2.21)>2 (H=2.10)>3 (H=1.89)>4 (H=1.84)>5 (H=1.65) whereas Simpson index value were varied at the points of 1 (D=0.14)<2 (D=0.17)<3,4 (D=0.18)<5 (D=0.19) respectively. Pollution and human interaction would have degraded the environmental quality of the mangrove habitats. Based on the observations it was found that the extent of disturbances was minimum at the points of 1 and 2 whereas anthropogenic influence was increased at the points of 3<4<5 respectively. Further, use of mangrove poles and sticks to build fish baits and fishing rods by the anglers could possibly destroy these small patches of mangroves. Hence, actions should be taken for conservation of existing patch and replenishment of new mangroves. 
 Keywords: Mangroves, Diversity, Shannon Diversity Index (H), Simpson Index (D) and Shannon Evenness (E)

Estimating aboveground volume of diverse urban tree species: Developing allometric equations for higher taxonomic levels

Allometric equations are essential for quantifying carbon storage in trees; however, owing to the high species diversity in urban green spaces, the development of allometric equations for all urban tree species is not feasible. In this study, we estimated the aboveground volume of urban trees and determined the fitness of several allometric equations. We compared the equations for different taxonomic levels, i.e., division, family, and species. A field survey was conducted across three types of urban green spaces (street trees, parks, and residential gardens) in three cities (Seoul, Goyang, and Wonju) in South Korea. We estimated the aboveground volumes of 1080 trees from their diameter measurements using a laser dendrometer, carried out at successive points from the ground to the top of the trees. Various tree size metrics, such as the diameter at breast height (DBH), height, and/or crown diameter, were applied as independent variable(s) in the equations. For gymnosperm trees, the allometric equations for higher taxonomic levels (RMSE: 0.011 m3) yielded goodness-of-fits similar to those at the species level (RMSE: 0.009 m3). For angiosperm trees, the family-level allometric equations satisfactorily covered the inter-species heterogeneity of tree shape, whereas the division-level allometric equations did not. The scaling exponent coefficients of allometric equations for each family (b = 1.65−2.69) linearly increased with an increasing mean height-to-DBH ratio. Although the allometric equations based on crown diameter were more unstable than those based on the DBH, integrating the height variable into the equations could ensure the feasibility of the allometric equations based on crown diameter. Our study facilitates the development of nationally specific emission factors for settlement areas and suggests the applicability of generalized or crown-based allometric equations for urban trees.

A multitaxonomic assessment of Natura 2000 effectiveness across European biogeographic regions.

The Natura 2000 (N2K) protected area (PA) network is a crucial tool to limit biodiversity loss in Europe. Despite covering 18% of EU's land area, its effectiveness at conserving biodiversity across taxa and biogeographic regions remains uncertain. Testing this effectiveness is, however, difficult as it requires considering the non-random location of PAs, and many possible confounding factors. Here, we used propensity score matching accounting for the confounding effects of biogeographic regions, terrain ruggedness, and land cover, when assessing the effectiveness of N2K PAs on the distribution of 1,769 priority species from EU's Birds and Habitats Directives, including mammals, birds, amphibians, reptiles, arthropods, fishes, molluscs, vascular and non-vascular plants. We compared alpha, beta, and gamma diversity between matched selections of protected and non-protected areas across EU's biogeographic regions using generalized linear models, generalized mixed models, and non-parametric tests for paired samples, respectively, for each taxonomic group and for the entire set of species. While we found N2K PAs to host significantly more priority species than non-protected land, this difference was not consistent across biogeographic regions and taxa. Total alpha diversity as well as alpha diversity of amphibians, arthropods, birds, mammals, and vascular plants were significantly higher inside PAs than outside, except in the Boreal region. Beta diversity was in general significantly higher inside N2K PAs than outside. Similarly, gamma diversity showed the highest values within N2K PAs, with some exceptions in Boreal and Atlantic regions. The planned expansion of the N2K network, as dictated by the European Biodiversity Strategy for 2030, should therefore target areas in the southern part of the Boreal region, areas with high species diversity of amphibians, arthropods, birds, mammals, and vascular plants, which are currently underrepresented in the N2K network. This article is protected by copyright. All rights reserved.

Fish Assemblage Concerning Physciochemical and Geomorphological Characteristics of Barak River, India

This study investigated the fish population, environmental characteristics, microhabitats, and soil erosion along the Barak River in Manipur and Assam. The survey covered 18 sites spanning from Maram in Senapati district, Manipur, to Badarpur in Karimganj district, Assam. The assessment involved analysing six physciochemical parameters and collecting fish specimens, focusing on human activities affecting different sections of the river. The environmental conditions varied significantly between two distinct geographical regions: the hilly area in Manipur and the plains in Assam. The river exhibited narrowness, shallow depths, rapid flow, and a substrate composed of stones and gravel in the hilly region of Manipur. Conversely, in the plains of Assam, the river was wider, slower flowing, deeper, and featured a substrate consisting of silt and clay. Bank erosion and river meandering were prevalent in the plains, which were densely populated and extensively cultivated. The survey documented a total of 35 fish species from 15 families and 7 orders. Interestingly, the distribution pattern indicated an absence of certain fish species in one region that were present in the other. The highest species diversity was recorded in Namtiram, Tamenglong district, Manipur, for the hilly stretch, and Srikona, Cachar district, Assam, for the plains. Surprisingly, the study noted that physciochemical parameters had minimal influence on fish distribution; instead, it appeared that habitat and other environmental factors played a more significant role in regulating the presence of fish species.

Unveiling coastal diversity: An inventory and conservation report of beach forest flora of Surigao del Sur, Philippines

Abstract. Ramos GB, Panduyos JB, Caray RE, Bacquial LS. 2024. Unveiling coastal diversity: An inventory and conservation report of beach forest flora of Surigao del Sur, Philippines. Biodiversitas 25: 421-430. Coastal greenbelts, comprise mangroves and beach forest trees, are pivotal in disaster mitigation and climate change resilience. Despite the evident importance of these coastal ecosystems, they are often overlooked in research due to a lack of comprehensive data on species community structure and their broader ecological importance. Hence, this present study was conducted to document beach forests composition and conservation status in selected coastal municipalities of Surigao del Sur, Philippines. The study was conducted in five sampling locations, namely, La Paz, Bil-at, Balibadon, Poblacion Cagwait and Arangasa Island. At each sampling location, a 2 km transect line, parallel to the beach’s shoreline, was established having a 100 square meter sampling plots spaced 50m apart. Beach species frequency counts, percentages, and diversity indices were quantified. The study documented 27 beach forest species belonging to 21 families and 25 genera with the dominant species being Crinum asiaticum, Cocos nucifera, Millettia pinnata, Timonius compressicaulis, and Terminalia catappa. Both Arangasa Island and La Paz beach forests have the highest species diversity (H’ = 2.04); however, Cagwait has the highest species richness (Margalef = 2.34). This study has potential to significantly contribute in addressing existing data gap regarding beach forest species in the Philippines. With the looming threat of extinction due to infrastructural development and anthropogenic activities, the study's outcomes stand as an urgent call to action; thus, a collaborative endeavor of Local Government Units, academicians, and community for conservation and protection before these species succumb to the threat of extinction.

Effect of litter decomposition on mowing and plant composition change during Solidago stand restoration

Decomposition of organic matter supports important soil ecosystem services. The rate of decomposition depends mostly on the type of plant material being decomposed and the abundance and diversity of organisms that process the organic matter. Consequently, any disturbance to the soil ecosystem will affect the decomposition process. Invasive plants, such as Solidago species, pose a serious ecological threat to natural habitats, so effective and environmentally safe methods of controlling their occurrence should be developed. In this study, decomposition rates were used as indicators of soil health during grassland restoration after Solidago invasion. Different seed mixtures (grasses, grasses with legumes, seeds collected from a seminatural meadow; use of fresh hay and no seeds) were sown during a field experiment and different mowing frequencies (1, 2 and 3 times per year) were established. Two hypotheses were tested: (1) plant species composition used in the restoration process affects litter decomposition rates, and (2) mowing regimes affect litter decomposition rates. It was found that decomposition rates were higher in plots with the highest species diversity. This indicates that an increase in species diversity has a positive effect on soil processes. Secondly, mowing two and three times per season has a positive effect on the decomposition process. In conclusion, decomposition rates can be used as a tool to identify adequate grassland management.

Morphological characterization of extrafloral nectaries in Brazilian Amazonian plant species

Background and Aims: Extrafloral nectaries (EFNs) are secretory glands of an aqueous solution composed mainly of sugars, amino acids and lipids, frequently used as food resource by ants, which tend to protect their host plants against herbivorous insects. Although the Amazon forest is recognized worldwide for its high plant diversity, few studies have been carried out in the region that characterize the different morphotypes of EFNs. In this context, our main aim was to perform a brief morphological description of EFNs in the Brazilian Amazonian rainforest. Methods: We established a plot of 6250 m2 in ten forest fragments situated in the state of Acre, Brazilian Amazon. In these plots, we performed intensive field surveys to locate EFN-bearing plant species. Subsequently, we used scanning electron microscopy images to morphologically characterize the EFNs. The morphological descriptions included information about the life form of EFN-bearing plant species, morphotypes of EFNs and their location in different plant structures. Key results: We morphologically characterized 67 EFN-bearing plant species, distributed in 28 genera and 19 families. The botanical families with the highest taxonomic representativeness were Fabaceae, Bignoniaceae and Malpighiaceae. Lianas were the life form with the largest number of EFN-bearing plant species, while elevated EFNs were the most frequent morphotype. Conclusions: The high frequency of elevated EFNs evidences the importance of the ant defense system against herbivory in these Brazilian Amazonian forests, since it has been documented in others works that this morphotype secretes a larger nectar volume, which is an advantage in relation to the attraction potential. Finally, our results indicate the existence of a high diversity of EFN-bearing plant species in this region of the Brazilian Amazon, and an important morphological diversity of associated extrafloral nectaries.

Interrelationships among litter chemistry, plant species diversity, and litter decomposition in tropical stream environments: a review

Factors that may accelerate decomposition are important for ecosystem functioning since plant litter decomposition is essential for carbon and nutrient cycling, but it is a generally slow process, which can take weeks up to years. In this context, studies have demonstrated that the chemical characteristics of litter mixtures can accelerate decomposition through several mechanisms. Tropical riparian forests are known for their high diversity of tree species, which leads to a wide array of litter types in tropical streams, each with distinct chemical properties. This underscores the key role of litter chemistry in significantly influencing the litter breakdown rate within of these streams. Here, we explore the interplay among litter chemistry, plant species diversity, and litter decomposition in tropical streams. We highlight the importance of litter physical and chemical characteristics for decomposition, as well as of the preservation of the natural floristic composition of tropical riparian forests. In this sense, more attention must be paid to the influence that the environment and phylogeny may have on the phytochemical characteristics of riparian forest plant species in different tropic biomes, and how the insertion of different exotic species interferes with the decomposition process. Furthermore, we emphasize the need for additional research into the consequences of the loss of rare plant species with unique functional characteristics to decomposition in tropical ecosystems.

Diversity of insect galls in veredas of the Brazilian Cerrado in Minas Gerais, Brazil

Brazilian veredas are hygrophilous communities with high species diversity, featuring many floristic studies but a still incipient number of faunistic studies. In the present study, we conducted an inventory of insect galls in four different veredas located in the Northern region of Minas Gerais, Brazil. Overall, we found 75 gall morphotypes, distributed across 50 host species representing 37 plant genera and 22 families. Fabaceae was the plant family with the greatest number of insect gall morphotypes (n = 21), followed by Malpighiaceae (n = 10). The plant genera that supported the highest diversity of insect galls were Copaifera (n = 8) and Byrsomima (n = 5). The plant species Copaifera oblongifolia (n = 6) and Anacardium humile (n = 4) exhibited the highest richness of insect galls. The leaves were the most attacked organs (89% of all galls). Most morphotypes are glabrous (83%), green (69%) and lenticular (60%). The taxa of gall-inducing insects were identified for 46 morphotypes (80%), and were recorded inducers from the orders Diptera, Lepidoptera, and Hymenoptera. Cecidomyiidae (Diptera) was the most representative galling group, with 42 morphotypes, making up 93.3% of the recorded inducers. Among the sampled veredas areas, the Vereda do Peruaçu presented the highest richness of insect gall morphotypes and host plant species. The faunistic similarity was higher in the Pedras and Tiririca veredas, followed by Almescla and Peruaçu veredas. This is the first systematic inventory of insect galls and their host plant in Brazilian veredas. The diversity of insect galls in the studied veredas is relatively high when compared to other Brazilian Cerrado vegetation types.

Height-diameter model of broad-leaved mixed forest based on species classification in Maoershan, Northeast China.

The complex stand structure and high species diversity of natural forests pose great challenges for analyzing stand growth and formulating reasonable plans for forest management. The height-diameter relationship is of great significance for predicting stand growth and formulating forest management measures. Based on survey data of 48 broad-leaved mixed forest plots in Maoershan, we classified 23 tree species into four groups based on species structure, growth characteristics and bionomics. We established a generalized model including stand, tree competition, species mixing and species diversity variables by reparameterization method, and a two-level mixed effect model of plot and tree species group. We tested the prediction ability of the model by leave-one-out cross-validation method. The results showed that the Ratkowsky (1990) model was the optimal basic model. The introduction of dominant height, basal area of trees larger than the object tree, basal area proportion of each species, and Shannon index could better explain the height-diameter relationship of broad-leaved mixed forest in Maoershan. The introduction of the mixed effect model of plot and tree species group could significantly improve the prediction accuracy of the model, with a Ra2 of 0.83. Under the same gradient of environmental factors, intolerant tree species exhibited higher tree heights than shade-tolerant tree species. In this study, we used the constructed tree height-diameter model to analyze the effects of species mixing and tree functional traits on tree height, which provided a theoretical basis for accurately predicting height of different tree species and analyzing the growth relationships in broadleaved mixed forests.

Woody Species Composition, Structure, Regeneration Status and Carbon Storage of Mkulazi Forest Reserve in Tanzania

Little is known about the status of forest condition and carbon storage potential of Mkulazi forest reserve (MFR) located in Morogoro District, Tanzania. This study was conducted to assess i) woody species composition, richness and diversity, ii) stand structure, iii) regeneration status and iv) carbon storage. Data collection for vegetation involved establishment of 100 concentric circular sample plots in the forest area of 65,710 ha. A total of 54 plant species for trees and shrubs with DBH ≥ 5 cm that belongs to 20 plant families were identified. The diversity of woody species was high (H’ = 3.11), while Stem density was 336 ± 126 stems ha-1, basal area was 9.48 ± 2.88 m2ha-1 and stand volume was 96.22 ± 32.51 m3ha-1. For the regeneration with DBH < 5 cm, a total of 26 plant species belonging to 11 plant families were identified. The diversity of woody species was also high (H’ = 3.14) and stem density was 1,198 ± 847 stems ha-1. The mean carbon stocks above ground for trees and shrubs with DBH ≥ 5 cm were 32.13 ± 10.91 Mg C ha-1 and that of below ground were 11.84 ± 3.58 Mg C ha-1. The observed high diversity of woody species, regeneration status and relatively high carbon storage potential signifies the importance of continuing protecting this reserve.

Cryptosporidium species and subtypes identified in human domestic cases through the national microbiological surveillance programme in Sweden from 2018 to 2022

BackgroundThe intestinal protozoan parasite Cryptosporidium is an important cause of diarrheal disease worldwide. A national microbiological surveillance programme was implemented in Sweden in 2018 in order to increase knowledge of the molecular epidemiology of human cryptosporidiosis to better understand transmission patterns and potential zoonotic sources. This article summarises the results of the first five years of the surveillance programme.MethodsCryptosporidium-positive faecal and DNA samples from domestically acquired infections were collected from clinical microbiological laboratories in Sweden. Species and subtype determination was performed using 60 kDa glycoprotein and/or small subunit ribosomal RNA gene analysis.ResultsBetween 2018 and 2022, 1654 samples were analysed and 11 different species were identified: C. parvum (n = 1412), C. mortiferum (n = 59), C. hominis (n = 56), C. erinacei (n = 11), C. cuniculus (n = 5), C. meleagridis (n = 3), C. equi (n = 2), C. ubiquitum (n = 2), and one each of C. canis, C. ditrichi and C. felis. Subtyping revealed seven subtype families of C. parvum (new subtype families IIy and IIz) and 69 different subtypes (11 new subtypes). The most common C. parvum subtypes were IIdA22G1c, IIdA24G1, IIdA15G2R1 and IIaA16G1R1b. For C. hominis, four different subtype families and nine different subtypes (two new subtypes) were identified. For additional species, two new subtype families (IIIk and VId) and nine new subtypes were identified. All successfully subtyped C. mortiferum cases were subtype XIVaA20G2T1, confirming previous findings in Sweden. Several outbreaks were identified of which the majority were foodborne and a few were due to direct contact with infected animals.ConclusionInfection with C. parvum is the leading cause of human cryptosporidiosis acquired in Sweden, where more than 90% of domestic cases are caused by this zoonotic species and only a small proportion of cases are due to infection with other species. The rodent-associated C. mortiferum is considered an emerging zoonotic species in Sweden and the number of domestically acquired human cases has surpassed that of infection with C. hominis. A high diversity of species and subtypes, as well as diversity within the same subtype, was detected. Also, cryptosporidiosis appears to affect adults to a great extent in Sweden.

Two new kateretid beetle species (Coleoptera: Kateretidae) with specialized antennal scapes in males from mid-Cretaceous Kachin amber

Kateretidae is a beetle family with 15 reported fossil species, four of them with specialized antennal scape. Specialized scape represents a rare form of sexual dimorphism in Kateretidae. Here we report two new species with a special scape in males of Protokateretes Zhao, Huang and Cai, 2023, and one female individual of an unnamed species of Protokateretes. The discovery of new species provides a supplementary diagnosis of Protokateretes, and increases the biodiversity of the fossil genus. The high diversity of species in Protokateretes provides new insights into sexual dimorphism of Kateretidae in mid-Cretaceous, enriching the knowledge of potential courtship strategy of kateretid beetles in the late Mesozoic.

Rays in the Shadows: Batoid Diversity, Occurrence, and Conservation Status in Fiji.

Over recent decades, elasmobranchs (sharks, rays, and skates) have been increasingly recognized among the world's most threatened marine wildlife, leading to heightened scientific attention. However, batoids (rays and skates) are relatively understudied, especially in Large Ocean States of the Pacific. This synthesis compiles insights on batoid diversity and occurrence in Fiji's waters by integrating a literature review, participatory science programs such as the Great Fiji Shark Count (GFSC) Initiative, Projects Abroad Fiji (PA), Manta Project Fiji (MPF), and iNaturalist, along with environmental DNA. Nineteen batoid species from seven families were identified: 19 species from the literature, 12 from participatory science programs, and six from eDNA analysis. Notably, this study provides the first photographic evidence for the bentfin devil ray (Mobula thurstoni, Lloyd, 1908) in Fiji. GFSC data indicated the highest species diversity in the Western Division, with spotted eagle rays (Aetobatus ocellatus, Kuhl, 1823) and maskrays (Neotrygon sp.) being observed most. In-person interviews conducted by PA provided information on the occurrence of wedgefishes and potentially sawfishes. MPF records and iNaturalist uploads were dominated by reef manta rays (M. alfredi, Krefft, 1868), while the pink whipray (Pateobatis fai, Jordan and Seale, 1906) yielded the most DNA sequences. Overall, 68.4% of the species face an elevated extinction risk based on the International Union for the Conservation of Nature Red List criteria. Although caution is warranted with older literature-based records for the giant guitarfish (Glaucostegus typus, Anonymous [Bennett], 1830), giant stingaree (Plesiobatis daviesi, Wallace, 1967), and the lack of sawfish verification, this synthesis highlights the effectiveness of a combined methodological approach in establishing a reference point for the diversity and occurrence of this understudied taxon in Fiji.

Microalgae biomass as a conditioner and regulator of soil quality and fertility.

Characteristics of an acid soil cultivated with Urochloa brizantha cv. Marandu were evaluated in relation to two types of fertilization: a conventional one, chemical based on nitrogen and potassium, and a biofertilizer, based on microalgae biomass. The results were compared among three treatments, control, conventional, and biological fertilization, with seven replications each. The study evaluated microalgae community, total carbon and nitrogen contents, mineral nitrogen, and enzymatic activity. Chlorella vulgaris showed the highest organism density, which can be explained by its rapid growth and high resistance. The highest species diversity was detected in the control 1,380,938 org cm-3 and biological 1,841,250 org cm-3 treatments, with the latter showing a higher density of cyanobacteria, especially Pseudanabaena limnetica with 394,554 org cm-3. The soil treated with chemical fertilization showed higher nitrate (9.14 mg NKg-1 NO3--N) and potassium (52.32 mg dm-3) contents. The highest levels of sulfur (21.73 mg dm-3) and iron (96.46 mgdm-3) were detected in the biological treatment. The chemical treatment showed higher activity of the enzymes acid phosphatase, acetylglucosaminidase, and sulfatase, while α-glucosidase and leucine aminopeptidase stood out in the biological treatment. Soil properties were not significantly affected by the treatments. The use of microalgae biomass derived from wastewater treatment from milking parlors was evaluated and presented as a promising biofertilizer for agriculture, following the line of recovering nutrient-rich wastes. In this sense, although many challenges need to be overcome, the results suggest that microalgal-based fertilizers could lead to low-impact agriculture.

Correlates of Bird Collection Compositions in Thai Zoos: Implications for Conservation and Management

Zoo collection management is increasingly driven by meeting global conservation needs. Many avian species have experienced population declines throughout Southeast Asia, underscoring the importance of ex situ conservation in these countries. We focus on Thailand, a bird diversity hotspot with a long tradition of keeping birds in captive settings. We aimed to understand what drives species acquisition and maintenance in Thai zoos. To that end, we surveyed 55 zoos, making a complete inventory of reptiles, birds, and mammals on display. We recorded 249 bird species, of which 149 are not native to Thailand. Bird species diversity was positively correlated with mammal species diversity but not with the entry ticket price, the Gross Domestic Product of the province in which the zoo was based, or the size of the zoo. Diversity did differ significantly between zoo types (accredited, government and private zoos). There was a clear difference in the proportion of native and non-native species between zoos, with private zoos containing the highest number of non-native species, which may be related to the licensing status of these zoos. The composition of bird species in Thai zoos appears to be largely driven by their availability, the legal status for keeping them and serendipity. The conservation status seems to be of minor importance, contradicting the typical role of a zoo. To be considered global conservation players, zoos in countries of high species diversity, such as Thailand, have the unique opportunity to provide breeding programmes for some of the rarest species, yet they must improve their collection management plans to focus on such aims.