Accessing tree species information is required for making appropriate decisions in forest management. 3D photo-interpretation using high spatial resolution aerial imagery is used to provide information on tree species in the province of Quebec. However, the shortage of qualified interpreters and the increasing costs of 3D photo-interpretation have affected the production of the forest inventory. In this study, we employed deep and machine learning models to classify nine tree species (i.e., paper birch, yellow birch, red maple, poplar, black spruce, white spruce, tamarack, jack pine, and balsam fir). We used a combination of spectral and vertical structural information extracted from 30-cm aerial imagery and airborne light detection and ranging (LiDAR) data in a 9 , 100 km 2 forested area in Quebec, Canada. The results indicated that Dense Convolution Network (DenseNet) achieved the best overall accuracy of 78%, outperforming machine learning methods by 5%. In addition, the models’ performance was independently assessed using permanent and temporary ground sample plots, acknowledging the superiority of DenseNet in terms of overall accuracy (73%) in predicting the dominant species. Our results suggest that the combination of aerial imagery and airborne LiDAR data, using deep learning approaches, can be applied to accurately map tree species.