You have accessJournal of UrologyKidney Cancer: Basic Research (III)1 Apr 2013471 ANALYSIS OF MOLECULAR PROFILING OF RENAL-CELL CARCINOMA (RCC). IDENTIFICATION OF A 4-MICRORNA SIGNATURE AS A PROGNOSTIC VALUE IN PATIENTS WITH STAGE I-II Felipe Villacampa, Angel Tejido, Felix Guerrero, Irma Ospina, Jose Manuel Duarte, Victor Martinez Silva, Guillermo de Velasco, Alfredo Rodriguez Antolin, Juan Passas, Federico de la Rosa, Daniel Castellano, and Rafael Diaz Gonzalez Felipe VillacampaFelipe Villacampa Madrid, Spain More articles by this author , Angel TejidoAngel Tejido Madrid, Spain More articles by this author , Felix GuerreroFelix Guerrero Madrid, Spain More articles by this author , Irma OspinaIrma Ospina Madrid, Spain More articles by this author , Jose Manuel DuarteJose Manuel Duarte Madrid, Spain More articles by this author , Victor Martinez SilvaVictor Martinez Silva Madrid, Spain More articles by this author , Guillermo de VelascoGuillermo de Velasco Madrid, Spain More articles by this author , Alfredo Rodriguez AntolinAlfredo Rodriguez Antolin Madrid, Spain More articles by this author , Juan PassasJuan Passas Madrid, Spain More articles by this author , Federico de la RosaFederico de la Rosa Madrid, Spain More articles by this author , Daniel CastellanoDaniel Castellano Madrid, Spain More articles by this author , and Rafael Diaz GonzalezRafael Diaz Gonzalez Madrid, Spain More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1862AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Currently, there is a need to identify stage I/II RCC patients with high risk of relapse following nephrectomy, given that of these patients 20-25% of them relapse. MicroRNAs (or miRNAs) are a class of small noncoding RNAs that control gene expression by targeting mRNA and playing an important role as regulators of gene expression during tumorigenesis. Our objective is to define a miRNA expression profile associated with a high risk of relapse in early RCC. METHODS We analyze 180 pts (all stages) of a local data-base with confirmed RCC histology who had undergone nephrectomy from 2000 to 2008. RNA was extracted (168 pts) from FFPE (Formalin-Fixed, Parafin-Embeded) samples using RecoverAll (Ambion). RNA samples were hybridized to Human miRNA Microarray Release 14.0, 8x15K (Agilent Technologies). We identified 113 pts with stage I-II pts. Only 396 miRNAs with detectable signal in at least 10% of the hybridized samples were considered for further analysis. Identification of miRNAs related with recurrence risk and subsequent developing and validation of miRNA expression-based prediction models of recurrence risk were performed in BRB-ArrayTools v4.2.1. RESULTS We identified a 4-miRNA expression signature that distinguishes early stage RCC patients with low and high recurrence risk (p value = 0.0013; HR = 4.68 (1.82-12.0)). Distant recurrence free survival rate at five years was 97.4 and 81.2 for the low and high recurrence risk groups respectively. High levels of miR-424 were related with a high recurrence risk (p=0.023). MiR-424 is a hypoxia induced miRNA whose expression has been related with HIF-1α and HIF-2α stabilization and angiogenesis induction. CONCLUSIONS Our results suggest that specific miRNAs are involved in the recurrence of early disease. We have found a miRNA expression signature that identifies patients with high risk of developing distant metastasis using the FFPE sample of primary tumors. High expression levels of miR-424 were related with a high recurrence risk. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e193-e194 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Felipe Villacampa Madrid, Spain More articles by this author Angel Tejido Madrid, Spain More articles by this author Felix Guerrero Madrid, Spain More articles by this author Irma Ospina Madrid, Spain More articles by this author Jose Manuel Duarte Madrid, Spain More articles by this author Victor Martinez Silva Madrid, Spain More articles by this author Guillermo de Velasco Madrid, Spain More articles by this author Alfredo Rodriguez Antolin Madrid, Spain More articles by this author Juan Passas Madrid, Spain More articles by this author Federico de la Rosa Madrid, Spain More articles by this author Daniel Castellano Madrid, Spain More articles by this author Rafael Diaz Gonzalez Madrid, Spain More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...
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