High-protein Supplement Research Articles

Impact of High Rumen Undegraded Protein (HRUP) Supplementation to Blood Urea Nitrogen and Reproduction Performance in Early Lactation Dairy Cows

Impact of High Rumen Undegraded Protein (HRUP) Supplementation to Blood Urea Nitrogen and Reproduction Performance in Early Lactation Dairy Cows

Soldier use of dietary supplements, including protein and body building supplements, in a combat zone is different than use in garrison

United States Army personnel in garrison who are not deployed to combat theater report using dietary supplements (DSs) to promote health, increase physical and mental strength, and improve energy levels. Given the substantial physical and cognitive demands of combat, DS use may increase during deployment. This study compared DS use by garrison soldiers with DS use by personnel deployed to a combat theater in Afghanistan. Prevalence and patterns of DS use, demographic factors, and health behaviors were assessed by survey (deployed n = 221; garrison n = 1001). Eighty-two percent of deployed and 74% of garrison soldiers used DSs ≥ 1 time·week(-1). Logistic regression analyses, adjusted for significant demographic and health predictors of DS use, showed deployed personnel were more likely than garrison soldiers to use protein, amino acids, and combination products. Deployed females were more likely to use protein supplements and deployed males were more likely to use multivitamins, combination products, protein, and body building supplements than garrison respondents. Significantly more deployed (17%) than garrison (10%) personnel spent more than $50∙month(-1) on DSs. Higher protein supplement use among deployed personnel was associated with higher frequency of strength training and lower amounts of aerobic exercise for males but similar amounts of strength training and aerobic exercise for females. Protein supplements and combination products are used more frequently by deployed than garrison soldiers with the intent of enhancing strength and energy.

Improvement in Hepatic Steatosis, Visceral Adiposity, and Appetite by High Dietary Protein Supplementation

Introduction: Non-alcoholic fatty liver disease (NAFLD), along with obesity, diabetes, lipid dysregulation, and cardiovascular disease, define the metabolic syndrome. This has reached epidemic proportions in the Unites States, affecting ˜30% of the population, and is one of the major disease states with an increasingprevalence. NAFLD ranges from simple steatosis to inflammatory, non-alcoholic, steatohepatitis (NASH), which can lead to fibrosis, cirrhosis, and eventual hepatocellular carcinoma (HCC). The long term effects of a high protein diet on the pathophysiology of NAFLD have not been previously examined. Aims: To examine the effects of high protein diets on body weight, composition, feeding behavior, orexigenic and anorexigenic metabolic hormones, visceral and liver fat composition. Methods: Male mice (C57Bl/6) were randomized into either three eucaloric (3.85 kcal/g) diet groups: Standard (10% fat, 70% CHO and 20% protein) (n=10), high fat ( 45% fat, 35% CHO and 20% protein kcal) (n=14) and high protein diet (calories: 10% fat, 30% carbohydrate and 60% protein) (n=8) for 12 weeks. Body wt, hepatic, visceral and peripheral fat measured bi-weekly using quantitative nuclear magnetic resonance. Feeding behavior microstructure was analyzed using the BioDAQ episodic food intake monitor for mice. Plasma levels of active ghrelin, GLP-1, PYY, insulin, glucagon, and leptin were analyzed both fasting and postprandial. Hepatic and visceral organs were analyzed by immunohistochemistry, and gene expression examined by qRT-PCR. Results: Mice on a HFD had a significantly greater amount of epididymal and hepatic fat whereas, mice on the HPD had a significantly lower amount of hepatic fat compared to either the standard or HFD. Fasting and postprandial leptin content was significant reduced in HPD whereas active-ghrelin was increased in HFD compared to standard diet. Immunohistochemistry revealed a significant reduction in hepatic steatosis and visceral fat, and de novo lipogenic and beta oxidative pathways were diminished by qRT-PCR. Conclusion: This is the first study to demonstrate that hepatic steatosis can be ameliorated by use of a high protein diet. These results establish the potential novel role of a HPD in the treatment of NAFLD and NASH perhaps through the release of gastrointestinal hormones.

ABNORMALITIES OF TESTICULAR FUNCTION OF MALE WISTAR RATS FED SOME PROTEIN SUPPLEMENTS

Athletes and bodybuilder consumed high protein supplements to obtain a lot of energy and enhance the development and strength of their muscles. However, after time, different investigations revealed dysfunctions of their body organs. There are contradictions between scientists concerning the benefits and the alarm of developing body dysfunction. In the present work, we selected three animal groups of male Wistar albino rats (n=10), control, hyper whey (HW) 2.6g/kg body weight and super amino 2500 (SA) 2.6g/kg body weight. The dose was provided through a healthy water tap for 14 weeks. The body weight was regularly measured. At the end of experiment, animals were sacrificed, dissected and blood collected from puncture of heart and the testes were removed and weighed. A biochemical analysis of function testes and histopathological investigations are done. From Morphometrical prospect, the total number of seminiferous tubules were markedly decreased in HW & SA. On the other hand, by using histopathological method on the symbol supplemented by WH, the spermatogenic cells were apparently declined and lacked normal pattern of arrangement. There was a noticeable increased number of necrotic spermatocytes and cell debris occupied the central region of the tubular lumina. Super amino supplementation revealed alterations of the seminiferous tubules. There was a clear reduction of spermatogenic cells. Biochemically. In HW and SA supplemented groups, it has registered a decrease in plasma total testosterone. The levels of T3 and T4 was markedly decreased in HW supplementation and slightly affected in SA fed rats

Effects of Exercise and Nutrition on the Coagulation System During Bedrest Immobilization.

Immobilization in hospitalized medical patients or during simulation of spaceflight induced deconditioning has been shown to be associated with loss of muscle mass and bone. Resistance vibrating exercise (RVE) and/or high protein diet are countermeasures, which are capable of mitigating the adverse effects of immobilization. We investigated the effect of these countermeasures on the coagulation system. Two groups of volunteers, each of whom performed such countermeasures, were enrolled in the study. Volunteers, who did nothing while bed rested, served as controls.The berest and the intervention protocols were carried out at Clinique d’ Investigation, MEDES, Toulouse, France.Eleven healthy men volunteered for this randomized crossover study. The subjects underwent 21 day of 6° head down bed rest (HDBR) followed by a washout period of 4 months. The first group followed an exercise schedule using resistance-vibrating exercise (RVE group). The second group also used the RVE but complemented it with high-protein supplement diet (NeX group). The third group only did bed rest.The highly sensitive methods calibrated automated thrombography (CAT) and thrombelastometry (TEM) were applied to monitor hemostatic changes.In all 3 groups, the hemostatic system shifted toward hypocoagulability during bed rest. For example, peak and thrombin formation velocity (VELINDEX) reduced in this period. Interestingly, a tendency toward hypercoagulation was observed during re-ambulation. In all 3 groups, ttPeak and StartTail were reduced, and Peak and VELINDEX (except in the RVE group) were significantly higher in relation to baseline values.Influence of bed rest on the coagulation system in the 2 groups performing countermeasures (RVE and NeX group) was the same as in the control bed-rested group. Clotting does not seem to be worsened by prolonged immobilization, or by countermeasures such as RVE/exercise or high-protein supplementation during immobilization. Therefore, only hospitalized medical patients at an elevated risk for thrombosis should be treated with anticoagulants. However, clinicians have to be aware that the re-ambulation period following immobilization might be associated with an elevated risk of thrombotic events.

P-219: High-protein oral nutritional supplements improve leg muscle quality in sarcopenic, malnourished adults

P-219: High-protein oral nutritional supplements improve leg muscle quality in sarcopenic, malnourished adults

Effect of high-protein supplemental therapy on subjective global assessment of CKD-5D patients.

Adequate nutrition in patients on hemodialysis is an important step for improving the quality of life. This prospective study was undertaken to monitor the nutritional status of patients who were given high-protein supplements on malnutrition inflammation score (MIS) and to correlate with biochemical parameters in maintenance hemodialysis (MHD) patients. This prospective study was conducted on 55 chronic kidney disease patients on MHD (37 women, 18 men), aged between 21 and 67 years. Of the 55 patients, 26 patients received high-protein commercial nutritional supplements, whereas 29 patients received high-protein kitchen feeding. Every patient had their MIS, 24-hour dietary recall, hand grip, mid arm circumference, triceps skin-fold thickness at 0, 3, and 6 months. Each of the above parameters was compared between the high-protein commercial nutritional supplement cohort and high-protein kitchen feeding cohort, and the data were analyzed. Of the 55 patients, 82.61% of patients on high-protein kitchen feeding group and 66.67% in high-protein commercial nutritional supplement group were nonvegetarian (P = 0.021). According to the MIS, improvement was observed in malnutrition status from 3- to 6-month period in 38.1% of patients in high-protein commercial supplement group, whereas only in 8.7% in high-protein kitchen feeding group (P = 0.04). Assessment showed improvement in malnutrition status with high-protein commercial nutritional supplement, which was marked in patients with age group >65 years (P = 0.03) and in those in whom serum albumin is <35 g/L (P = 0.02). Both high-protein kitchen feeding and high-protein commercial nutritional supplement cohorts were observed to have improvement in overall nutritional status. Older patients >65 years with lower serum albumin levels (<3.5 g/dL) were observed to have significant improvement in nutritional status with high-protein commercial nutritional supplements.

Antenatal dietary education and supplementation to increase energy and protein intake.

Antenatal dietary education and supplementation to increase energy and protein intake.

Milk-soluble formula increases food intake and reduces Il6 expression in elderly rat hypothalami.

Malnutrition in the elderly is accompanied by several metabolic dysfunctions, especially alterations in energy homeostasis regulation and a loss of insulin responsiveness. Nutritional recommendations aim to enrich food with high protein and energy supplements, and protein composition and lipid quality have been widely studied. Despite the numerous studies that have examined attempts to overcome malnutrition in the elderly through such nutritional supplementation, it is still necessary to study the effects of a combination of protein, lipids, and vitamin D (VitD). This can be done in animal models of elderly malnutrition. In the present study, we investigated the effects of several diet formulae on insulin responsiveness, inflammation, and the hypothalamic expression of key genes that are involved in energy homeostasis control. To mimic elderly malnutrition in humans, elderly Wistar rats were food restricted (R, -50%) for 12 weeks and then refed for 4 weeks with one of four different isocaloric diets: a control diet; a diet where milk soluble protein (MSP) replaced casein; a blend of milk fat, rapeseed, and DHA (MRD); or a full formula (FF) diet that combined MSP and a blend of MRD (FF). All of the refeeding diets contained VitD. We concluded that: (i) food restriction led to the upregulation of insulin receptor in liver and adipose tissue accompanied by increased Tnfα in the hypothalamus; (ii) in all of the refed groups, refeeding led to similar body weight gain during the refeeding period; and (iii) refeeding with MSP and MRD diets induced higher food intake on the fourth week of refeeding, and this increase was associated with reduced hypothalamic interleukin 6 expression.

Body Composition and Bone Mineral Density of a Female Body Builder Following 33 weeks of Pre-Contest Training

Marked changes in body composition (BC) often occur as body builders prepare for a contest. These changes are a result of nutritional manipulation, as well as intense and high volume exercise. Concern for the health and well being of the participant is warranted based on severe changes over several months. PURPOSE: To monitor changes in body composition and bone density (BMD) during a six month regimen of vigorous resistance (R), and cardiovascular training (C), a competitive body builder was monitored. METHODS: A 29 yr old female with 8 yrs of R experience was evaluated with Dual-Energy Xray Absorptiometry (iDEXA) for BC and BMD at 1, 24 and 32 weeks prior to competition. During the initial 22 wks she consumed an isocaloric diet and engaged in R and C at a frequency of 4-5 and 3 sessions/wk, respectively. The intensity (momentary muscular fatigue) and volume (90 min R, 45 min C) of workouts were similar throughout the study, but frequency increased to 6x/wk for R, and to 4x/wk for C, while during the final 10 wks (pre-competition) she ingested less than 1300 kcal/day (60% protein, 15% fat, 25% CHO). Her CHO intake was primarily ingested immediately prior to and during workouts. In addition, she ingested supplements of creatine monohydrate, BCAA, high protein and electrolytes both pre and post every R session. RESULTS:Table: No title available.Bone mineral content was maintained at 2.5 kg throughout the 32 weeks. In addition, regional % fat was reduced 42.9, 46.2, and 60%, in the arms, legs, and trunk from 24 to 1 wk pre competition, respectively. CONCLUSION: High intensity and high volume R and C training combined with limited dietary intake and high protein supplements can maintain BMD and LBM despite enormous reductions in body fat, body mass (BM) and regional fat distribution.

Effects of replacing extracted soybean meal with rapeseed cake in corn grass silage-based diet for dairy cows.

The aim of the study was to assess the effects of partial replacement of soybean meal with a protein-equivalent amount of rapeseed cake in the diet on milking parameters and fatty acid (FA) composition of milk in dairy cows. Two groups of Holstein-Friesian cows, 8 each, consisting of randomised blocks were studied: a control group (C) was given a traditional high-protein supplement (extracted soybean meal) and the experimental group (E), had part of extracted soybean meal replaced with rapeseed cake. Dry matter intake and milk yield in both groups were not affected by the diet but milk fat percentage and yield were decreased in both groups. Rapeseed cake had no effect on milk acidity or on protein (including casein) and lactose contents. A lower concentration of urea in milk in E group indicated a proper ratio of protein to energy in the fodder. Health condition of mammary gland and indicators of metabolic profile were not affected by rapeseed cake supplementation. In E group, the share of atherogenic saturated fatty acids (FA) was reduced after 11 weeks: palmitic, by 26% and myristic, by 22%; moreover, as compared with control cows, the content of monounsaturated FA in milk increased by 44% after 3 weeks and by 68% after 11 weeks, t-18:1 and c-9 t-11 isomer of CLA increased about 2.5-fold after 11 weeks. In E group, the atherogenic index (AI) was significantly (P < 0.001) lower than in C (by 54% on average) and the decrease with time was considerable (by 29%, P < 0.001). Contents of odd- and branched- chain FA in milk were not significantly affected thus reflecting proper rumen function. Partial replacement of soybean meal with rapeseed cake in the diet of cows may improve both milking indices and FA profile of milk.

Immune Potentiating role of Protein, Probiotic and Symbiotic Supplementation in Moulted White Leghorn Hens

This study investigated the effects of high protein, probiotic and symbiotic supplementation on the immunity of culled moulted White Leghorn hens. Cage-housed hens ( n = 240) aged about 70 weeks were moulted by including zinc in the diet. The moulted hens were randomly divided in four treatment groups: control group (G1; CP 16% in basal diet), high protein group (G2; CP 18% in basal diet), probiotic supplemented group (G3; symbiotic at a dose rate of 85 mg L−1 in drinking water + CP 16% in basal diet), and symbiotic group (G4; probiotic at a dose rate of 85 mg L−1 in drinking water + CP 16% in basal diet). Ten birds from each group were euthanised at 5% peak (5P), full peak (FP), and end of production (EP). Blood samples were taken for serum to estimate geometric mean antibody titre (GMT) against Newcastle disease virus (NDV) and egg drop syndrome (EDS) virus. Macrophages were isolated from abdominal exudates (AE) to observe their engulfment response and their nitric oxide (NO) production. 2,4-Dinitrochlorobenzene (DNCB) was inoculated on the skin of five birds in each group to evaluate the delayed type hypersensitivity response by determining the skin thickness. The overall humoral antibody GMT against NDV in the groups was higher in G2, G3 and G3 compared to G1. GMT value against EDS was higher in G2 compared to G1. A significant increase ( P ≤ 0.01) in macrophage engulfment and NO production was seen in G2 and G4 compared to the G1. The DNCB inoculated skin thickness response remained higher ( P ≤ 0.01) in G2 until FP than the other groups. A diet rich in protein or supplemented with a probiotic or a symbiotic enhances the body immunity of moulted hens.

Pre-partum supplementation increases first-lactation heifer re-conception rates in the Victoria River District, NT

Previous studies have reported inconsistent results when attempting to use pre-partum supplementation to improve re-conception rates in first-lactation heifers in northern Australia. It has been speculated that where no improvement in re-conception rates was found that it may have been because supplementation was stopped before the wet season began, and the benefits from supplementation were lost when heifers lost weight between the end of supplementation and the start of the wet season. This study was conducted to determine whether re-conception rates in Brahman first-lactation heifers in the Victoria River District (VRD) could be reliably improved by pre-partum supplementation with high protein supplements (at a rate of 0.4% of liveweight per day). Re-conception rates were significantly higher in supplemented (SUP) heifers in each of the 3 years of this study (re-conception rates were an average of 42 % units higher in SUP heifers over the 3 years). It was concluded that feeding pre-partum protein supplements for a period of at least 100 days until green grass is available at the start of the wet season is a reliable method of increasing re-conception rates in first-lactation heifers in the VRD. However, even with large increases in re-conception rate, the profitability of supplementation strategies in northern Australia need to be carefully evaluated due to high on-farm costs of supplements. Profitability is also adversely affected when the feeding period is extended due to a late start to the wet season.

Nutritional evaluation of young bulls on tropical pasture receiving supplements with different protein:carbohydrate ratios.

The objective of this work was to evaluate the nutritional parameters of young bulls supplemented with different ratios of protein: carbohydrate on tropical pastures from 4 until 18 months old. Fifty-five non-castrated beef calves (138.3±3.4 kg, 90 to 150 d of age) were used. The calves (young bulls) were subjected to a 430-d experimental period encompassing 4 seasons. The treatments were as follows: control, only mineral mixture; HPHC, high protein and high carbohydrate supplement; HPLC, high protein and low carbohydrate supplement; LPHC, low protein and high carbohydrate supplement; and LPLC, low protein and low carbohydrate supplement. The amount of supplement was adjusted every 28 d. Dry matter (DM) intake was higher in the dry-to-rainy transition and rainy seasons for all nutritional plans. Non-supplemented animals had lower intakes of DM and total digestible nutrients (TDN) than supplemented young bulls in all seasons. Although differences in DM intake were not observed between supplemented animals, the supplements with high carbohydrate (HPHC and LPHC) had lower forage intake during suckling (rainy-to-dry transition season) and in the rainy season. However, the HPHC treatment animals had higher intake and digestibility of neutral detergent fiber. It can be concluded that supplementation with high protein levels (supplying 50% of the crude protein requirement) provide the best nutritional parameters for grazing young bulls in most seasons, increasing intake and digestibility of diet, and these effects are more intense when associated with high carbohydrate levels level (supplying 30% TDN requirement).

&lt;b&gt;Body composition and deposition efficiency of protein and energy in grazing young bulls

The effects of supplementation with different protein: carbohydrate ratios on body composition, carcass characteristics and protein and energy deposition efficiency of young were assessed. Twenty-four Nellorecalves (132.5 ± 5.5 kgand 90-150 days of age) were kept on pasture for a 430 day experimental period. The treatments were: Control = mineral mixture only; HPHC = high-protein and high-carbohydrate supplement; HPLC = high-protein and low-carbohydrate supplement; LPHC = low-protein and high-carbohydrate supplement; LPLC = low-protein and low-carbohydrate supplement. Four animals at begning and 20 animal at end of experiment were slaughtered to evaluate the carcass composition. Control bulls had the lowest (p 0.05) between supplemented bulls (13 Mcal day -1 ). Although non-supplemented bulls had less (p 0.05) between supplemented bulls. High-carbohydrate supplements were associated with more (p 0.05) in the energy efficiency between the groups. Therefore, supplementation increases the intake and retention of protein and energy without changing the retention efficiency.

Forage and seed yield of winter turnip rape established as a mixed crop with cereals

SUMMARYCultivation of winter turnip rape (Brassica rapa L. ssp. oleifera (DC.) Metzg.) in Finland has been limited because of its reputation as an unreliable crop and its mid-season sowing time of July, when fields are already sown to other crops. An alternative management practice for winter turnip rape is proposed whereby it would be sown as a mixed crop simultaneously with spring cereals. The growth and yield formation of winter turnip rape grown in mixed stands with four different spring cereals was studied in two field experiments conducted in 2009–11. Pure and mixed stands of winter turnip rape and spring cereals were established in May at two different cereal and winter turnip rape stand densities. Subsequent to cereal harvest, one-third of each winter turnip rape plot was harvested for biomass in autumn, before cessation of growth. Three plant stand types, May- and July-sown monocrops and a mixed crop with oat (Avena sativa L.) were sampled for forage analysis. Plant stand densities were monitored from establishment until maturity. Winter turnip rape yield and its quality, including oil content, protein content and thousand seed weight, were determined. Following favourable overwintering conditions, winter turnip rape established with cereals yielded comparably to that of pure stands in terms of both quantity and quality. However, a pure stand of winter turnip rape out-yielded mixed crop stands after unfavourable overwintering conditions. Leaf removal decreased plant survival and seed yield. Establishing winter turnip rape with a cereal in May is an alternative to sowing it as a monocrop in July. A higher seeding rate is needed for under-sown winter turnip rape. Furthermore, autumn-harvested winter turnip rape monocrop forage potentially represents a high-protein supplement for ruminants.

Diet Quality Limits Summer Growth of Field Vole Populations

Marked variation occurs in both seasonal and multiannual population density peaks of northern European small mammal species, including voles. The availability of dietary proteins is a key factor limiting the population growth of herbivore species. The objective of this study is to investigate the degree to which protein availability influences the growth of increasing vole populations. We hypothesise that the summer growth of folivorous vole populations is positively associated with dietary protein availability. A field experiment was conducted over a summer reproductive period in 18 vegetated enclosures. Populations of field voles (Microtus agrestis) were randomised amongst three treatment groups: 1) food supplementation with ad libitum high protein (30% dry weight) pellets, 2) food supplementation with ad libitum low protein (1% dry weight; both supplemented foods had equivalent energy content) pellets, and 3) control (no food supplementation), n = 6 per treatment. Vole density, survival, demographic attributes and condition indicators were monitored with live-trapping and blood sampling. Highest final vole densities were attained in populations that received high protein supplementation and lowest in low protein populations. Control populations displayed intermediate densities. The survival rate of voles was similar in all treatment groups. The proportion of females, and of those that were pregnant or lactating, was highest in the high protein supplemented populations. This suggests that variation in reproductive, rather than survival rates of voles, accounted for density differences between the treatment groups. We found no clear association between population demography and individual physiological condition. Our results demonstrate that dietary protein availability limits vole population growth during the summer growing season. This suggests that the nutritional quality of forage may be an underestimated source of interannual variation in the density and growth rates of widely fluctuating populations of herbivorous small mammals.

Protein Supplements: Do They Alter Dietary Intakes?

Effects of protein versus mixed macronutrient supplementation on total energy intake (TEI) and protein intake during an ad libitum diet were examined. Trained males undertook two, 2-week dietary interventions which were randomized, double blinded, and separated by 2 weeks. These were high-protein supplementation (HP: 1034.5 kJ energy, 29.6 g protein, 8.7 g fat and 12.3 g CHO) and standard meal supplementation (SM: 1039 kJ energy, 9.9 g protein, 9.5 g fat, and 29.4 g CHO) consumed daily following a week of baseline measures. Eighteen participants finished both interventions and one only completed HP. TEI (mean ± SD) was not different between baseline (11148 ± 3347 kJ) and HP (10705 ± 3143 kJ) nor between baseline and SM (12381 ± 3877 kJ), however, TEI was greater with SM than HP (923 ± 4015 kJ p = .043). Protein intake (%TEI) was greater with HP (22.4 ± 6.2%) than baseline (19.4 ± 5.4%; p = .008) but not SM (20.0 ± 5.0%). No differences in absolute daily protein intake were found. Absolute CHO intake was greater with SM than HP (52.0 ± 89.5 g, p = .006). No differences in fat intake were found. Body mass did not change between baseline (82.7 ± 11.2 kg) and either HP (83.1 ± 11.7 kg) or SM (82.9 ± 11.0 kg). Protein supplementation increases the relative proportion of protein in the diet, but doesn't increase the absolute amount of total protein or energy consumed. Thus some compensation by a reduction in other foods occurs. This is in contrast to a mixed nutrient supplement, which does not alter the proportion of protein consumed but does increase TEI.

Relative Bile Acid Binding Potential of Extruded Lentil Snacks

Previously we have reported that extrusion significantly improved healthful potential of cereals. It was hypothesized that snacks produced by extrusion would be more healthful than their raw formulations. Bile acid binding has been reported to indicate cholesterol lowering and cancer risk reduction potential of food and fractions. Bile acid binding potential of five lentil snack raw formulations and their extruded snacks were evaluated. The raw formulations were 100% lentils (F01), 69% lentils (F02), 57% lentils + 12% supplement (F03), F03 with 125 μg/100g Chromium (F04), F03 with 536 μg/100g Chromium (F05), and their respective extruded (E) snacks E01, E02, E03, E04 and E05. The in vitro bile acid binding on an equal dry matter basis relative to cholestyramine, was F01 (0.5%), E01 (3.7%), F02 (0.6%), E02 (3.0%), F03 (1.6%), E03 (5.1%), F04 (2.0%), E04 (4.2%), F05 (0.8%) and E05 (3.6%). Replacing 12% lentils with high protein supplements (F02 vs. F03) resulted in significantly higher bile acid binding, suggesting that the supplement appears to have higher bile acid binding capacity than that of lentils. All the extruded lentil snacks had significantly higher bile acid binding compared with their raw formulations. Extruding with added chromium containing yeast resulted in significantly lower bile acid binding in a dose dependent manner. Most healthful lentil snacks were produced with the addition of high protein supplement without added chromium-containing yeast (E03). Data proved the hypothesis that lentil snacks produced by extrusion are significantly more healthful than their raw formulations.

Supplemental feeding during pregnancy compared with maternal supplementation during lactation does not affect schooling and cognitive development through late adolescence

Background: The long-term impact of early malnutrition on human capital outcomes remains unclear, and existing evidence has come largely from observational studies.Objective: We compared the impact of a nutritional supplement given during pregnancy or lactation in rural Gambia on educational performance and cognitive ability in offspring at their maturity.Design: This study was a follow-up of a randomized trial of prenatal high protein and energy supplementation conducted between 1989 and 1994. Subjects were 16–22 y of age at follow-up, and information was collected on schooling achievement and cognitive ability by using the Raven's progressive matrices test, Mill Hill vocabulary test, and forward and backward digit-span tests.Results: A total of 1459 individuals were traced and interviewed and represented 71% of the original cohort and 81% of the surviving cohort. There was no difference in cognitive ability or educational attainment between treatment groups by using any of the methods of assessment.Conclusion: We have shown little evidence to support a long-term effect of prenatal protein-energy supplementation compared with supplementation during lactation on cognitive development in rural Gambians. This trial was registered at http://www.controlled-trials.com as ISRCTN72582014.