High Protein Group Research Articles

Estimation of biochemical and technological indicators of grain of hulled and hulles barley varieties in the conditions of the Middle Volga region

In order to use efficiently barley varieties for such purposes as food, fodder and brewing, an objective and thorough study of grain quality is of great necessity, with adjustment for the features of hulles varieties. The purpose of the current study was to estimate the biochemical and technological indicators of grain varieties of hulled and hulles barley, to determine the correlation between quality indicators. On the experimental basis of the Samara Research Institute of Agriculture, there were studied grain quality indicators of hulled and hulles barley varieties of the Competitive Variety Testing in 2019–2021. There has been established a high-protein group of varieties, such as ‘Orlan’, ‘Bezenchuksky 2’, ‘Lun’, ‘Streletsky golozerny’ and ‘Omsky golozerny 1’ with 14.9–16.8 % of protein. The largest ‘1000-grain weight’ (43.7–44.1 g) and low activity of α-amylase in grain (352–421 c of ‘falling number’) over the years of study was established among the hulled barley varieties of this group. Barley varieties with such qualitative traits could be widely used in the food industry and feed production. There has been noted that hulles barley varieties with a high protein percentage (16.8 %), the absence of hulls and low amylolytic activity (471 c), when adapting to growing conditions, will improve not only the quality of cereals, grain fodder, but can also be used for baking purposes. When summarizing the correlation analysis results, there was established that the correlation between a grain protein index and other qualitative traits was identified only under the extremely dry conditions of 2021. Grain husk content closely correlated inversely with grain unit (–0.94**; –0.64*; –0.84**) through all years of study. The ‘falling number’ in 2019 was somewhat less correlated with 1000-grain weight (0.60*) and grain unit (0.61*); negatively correlated with grain husk content both in 2019 and 2020 (–0.65*; –0.82**).

The effect of higher protein dosing in critically ill patients with high nutritional risk (EFFORT Protein): an international, multicentre, pragmatic, registry-based randomised trial

On the basis of low-quality evidence, international critical care nutrition guidelines recommend a wide range of protein doses. The effect of delivering high-dose protein during critical illness is unknown. We aimed to test the hypothesis that a higher dose of protein provided to critically ill patients would improve their clinical outcomes. This international, investigator-initiated, pragmatic, registry-based, single-blinded, randomised trial was undertaken in 85 intensive care units (ICUs) across 16 countries. We enrolled nutritionally high-risk adults (≥18 years) undergoing mechanical ventilation to compare prescribing high-dose protein (≥2·2 g/kg per day) with usual dose protein (≤1·2 g/kg per day) started within 96 h of ICU admission and continued for up to 28 days or death or transition to oral feeding. Participants were randomly allocated (1:1) to high-dose protein or usual dose protein, stratified by site. As site personnel were involved in both prescribing and delivering protein dose, it was not possible to blind clinicians, but patients were not made aware of the treatment assignment. The primary efficacy outcome was time-to-discharge-alive from hospital up to 60 days after ICU admission and the secondary outcome was 60-day morality. Patients were analysed in the group to which they were randomly assigned regardless of study compliance, although patients who dropped out of the study before receiving the study intervention were excluded. This study is registered with ClinicalTrials.gov, NCT03160547. Between Jan 17, 2018, and Dec 3, 2021, 1329 patients were randomised and 1301 (97·9%) were included in the analysis (645 in the high-dose protein group and 656 in usual dose group). By 60 days after randomisation, the cumulative incidence of alive hospital discharge was 46·1% (95 CI 42·0%-50·1%) in the high-dose compared with 50·2% (46·0%-54·3%) in the usual dose protein group (hazard ratio 0·91, 95% CI 0·77-1·07; p=0·27). The 60-day mortality rate was 34·6% (222 of 642) in the high dose protein group compared with 32·1% (208 of 648) in the usual dose protein group (relative risk 1·08, 95% CI 0·92-1·26). There appeared to be a subgroup effect with higher protein provision being particularly harmful in patients with acute kidney injury and higher organ failure scores at baseline. Delivery of higher doses of protein to mechanically ventilated critically ill patients did not improve the time-to-discharge-alive from hospital and might have worsened outcomes for patients with acute kidney injury and high organ failure scores. None.

Effects of Different Dietary Protein Level on Growth Performance, Rumen Fermentation Characteristics and Plasma Metabolomics Profile of Growing Yak in the Cold Season.

This experiment was aimed to compare the effects of two diets with different protein content on the growth performance, immune indexes, rumen fermentation characteristics and plasma metabolomics of growing yak in the cold season. A total of 24, 2-year-old healthy yaks with similar body weight (142.9 ± 3.56 kg) were randomly allocated to two isoenergetic diets with different protein content (10 vs 14%) according to a non-paired experimental design, and the protein of the diets was increased by increasing soybean meal, rapeseed meal and cottonseed meal. The growth performance experiment lasted 56 days. Four days before the end of the growth experiment, the digestion trial was conducted, and the rumen fluid and plasma was collected for measurement. The results showed that the average daily feed intake (p < 0.001) and average daily gain (p = 0.006) of yak fed a high-protein diet was significantly greater, while the feed conversion ratio was lower (p = 0.021) than that of yaks fed a low-protein diet. Plasma aspartate aminotransferase (p = 0.002), alanine aminotransferase (p < 0.001), malondialdehyde (p = 0.001), tumor necrosis factor-α (p = 0.032) and interferon-γ (p = 0.017) of the high-protein group were significantly lesser, whereas superoxide dismutase (p = 0.004) and interleukin-2 (p = 0.007) was significantly greater than that of the low-protein group. The rumen microbial crude protein (p < 0.047) and crude protein digestibility (p = 0.015) of yak fed a high-protein diet was significantly greater than that of the low-protein group. The metabolomics results showed that yaks fed a high-protein diet were elevated in protein digestion and absorption, arginine and proline metabolism, tryptophan metabolism, purine metabolism, butanoate metabolism, taste transduction, pyrimidine metabolism, pantothenate and CoA biosynthesis, glutathione metabolism and renin secretion pathways. It is concluded that a high-protein diet in the cold season can promote rumen microbial crude protein synthesis, enhance antioxidant and immune function and promote growth performance of yaks.

The effect of protein enriched nutrition on diaphragm function in mechanically ventilated patients

ObjectivesOur primary aim was to demonstrate the effect of high protein on diaphragm muscle thickness, our secondary aim was to evaluate the correlation of diaphragm thickness fraction with rectus femoris muscle thickness in high protein patients. Patients and methodsThis study included 49 mechanically ventilated patients who were randomly divided into a standard protein (1.2g/kg/d) group and high protein group (2.0g/kg/d). Thickness of rectus femoris, thickness of peak inspiratory diaphragm, thickness of end expiratory diaphragm were measured by ultrasonography. Thickness fraction of diaphragm was calculated. ResultsIn the high protein group, thickness of peak inspiratory diaphragm values were found to be statistically significantly higher on days 3rd, 5th, 7th, and 15th (P<0.05). Thicknesses of end-expiratory diaphragm values were found to be statistically significantly higher on the 3rd, 5th and 7th days (P<0.05). Thicknesses fraction of diaphragm values were found to be statistically significantly higher on days 3rd, 5th, 7th and 15th (P<0.05) in the high protein group. Thicknesses of rectus femoris values were higher in the high protein group compared with standard protein group on the 5th, 7th, and 15th days (P<0.005). In high protein group, statistically significant correlations were found between thickness of rectus femoris and thickness fraction of diaphragm at the 3rd day (rho=0.429, P=0.032). ConclusionThis study showed that diaphragmatic muscle thicknesses were higher in patients who received 2g/kg/d protein supplement, These findings have shown that muscle atrophy can be prevented with high protein support in the intensive care unit.

Milk-cereal mix supplementation during infancy and impact on neurodevelopmental outcomes at 12 and 24 months of age: a randomized controlled trial in India.

Inadequate protein intake and lack of micronutrients may affect neurodevelopment in infants. This randomized controlled trial was conducted to measure the effect of two milk-cereal mixes with modest and high amounts of protein and enriched with multiple micronutrients (MMN), given between 6-12 months, on cognitive, language, motor and behavioural scores at 12 and 24 months of age, compared to no-supplementation. The two supplements were also compared with each other. The study was conducted in urban Delhi, India and the infants were randomized in a 1:1:1 ratio to the three study groups. At 12 and 24 months of age, 1134 and 1214 children were available, respectively. At 12 months of age, compared to no supplement group, an increase in the motor scores (mean difference, MD 1.52, 95% CI: 0.28, 2.75) and a decrease in the infant temperament scores (mean difference, MD -2.76, 95% CI: -4.23, -1.29) in the modest protein group was observed. Those in the high protein group had lower socio-emotional scores (MD -1.40, 95% CI: -2.43, -0.37) and higher scores on infant temperament scale (MD 2.05, 95% CI: 0.62, 3.48) when compared to modest protein group. At 24 months, no significant differences in any of the neurodevelopment scores between the three study groups was found. In conclusion, supplementation with modest amount of protein and MMN may lead to short term small improvements in motor function and infant temperament. There appears no advantage of supplementing with high protein, rather negative effects on infant behaviour were observed.

High-sensitivity C-reactive protein use in cardiovascular risk screening at 6 to 12 months postpartum following hypertensive disorders of pregnancy

Patients with a hypertensive disorder of pregnancy are more likely to have underlying cardiovascular risk factors and are at increased risk of future cardiovascular disease. These patients are more likely to be diagnosed with new-onset chronic hypertension and meet the criteria for metabolic syndrome postpartum. High-sensitivity C-reactive protein is a marker of general inflammation and may be used to identify increased risk for cardiovascular disease. This collaborative data-sharing study between Yale University, United States (Yale Hearts Moms study) and Queen's University, Canada (Maternal Health Clinic) aimed to study the utility of high-sensitivity C-reactive protein in postpartum cardiovascular risk screening, as determined by 30-year risk (Framingham) and metabolic syndrome 6 to 12 months postpartum. Patients with a hypertensive disorder of pregnancy (n=478) or an uncomplicated, term pregnancy (n=90) had cardiovascular risk screening and risk scoring performed at 6 to 12 months postpartum. Patients were excluded if they had a multiple gestation or chronic hypertension, diabetes mellitus, or cardiovascular disease diagnosed before pregnancy. Patients were categorized according to high-sensitivity C-reactive protein (mg/L) into Normal (<3.0), High (3.1 to <10.0), and Acute (≥10.0) groups. The primary outcome of the study was risk for future cardiovascular events, calculated through surrogate measures such as hypertension and cholesterol. Kruskal-Wallis and chi-square tests were used to compare groups, with post hoc tests corrected using the Bonferroni method. Multivariable logistic regression was used to assess the association between high-sensitivity C-reactive protein and cardiovascular risk, adjusting for relevant medical and sociodemographic variables. Analysis was completed with IBM SPSS Statistics, version 27. Patients in the High and Acute high-sensitivity C-reactive protein groups were more likely to have a body mass index ≥30, to have experienced a hypertensive disorder of pregnancy, to have a lower household income, and to have not breastfed or to have breastfed for <6 months, when compared with the Normal high-sensitivity C-reactive protein group (all P<.05). Patients in the High and Acute high-sensitivity C-reactive protein groups had higher 30-year cardiovascular risk scores and were more likely to have metabolic syndrome when compared with the Normal high-sensitivity C-reactive protein group (all P<.05). Patients with High high-sensitivity C-reactive protein had 2-fold odds of metabolic syndrome 6 to 12 months after delivery, compared with those in the Normal high-sensitivity C-reactive protein group (adjusted odds ratio, 2.85 [95% confidence interval, 1.66-4.91]), adjusting for hypertensive disorder of pregnancy, body mass index, clinic site, breastfeeding, income, and family history of cardiovascular disease. Those with Acute high-sensitivity C-reactive protein also seemed to have elevated odds of metabolic syndrome compared with the Normal high-sensitivity C-reactive protein group (adjusted odds ratio, 2.52 [95% confidence interval, 1.24-5.12]). The odds of chronic hypertension were significantly higher (P<.05) in the High high-sensitivity C-reactive protein group (adjusted odds ratio, 1.72 [95% confidence interval, 1.12-2.65]) compared with the Normal group. Individuals with elevated postpartum high-sensitivity C-reactive protein are at increased risk of cardiovascular disease 6 to 12 months postpartum after a pregnancy complicated by a hypertensive disorder of pregnancy. Future research is critical to determine the most comprehensive and accurate method and timing of postpartum cardiovascular risk screening to decrease the incidence of preventable cardiovascular mortality among women.

Impact of postnatal nutrition on neurodevelopmental outcome in rat model of intrauterine growth restriction.

There are limited data on nutritional management of infants with intrauterine growth restriction (IUGR). Postnatal protein supplementation for promoting growth is a common clinical practice in neonatology. The present study aims to investigate the consequences of protein supplementation on long-term growth, brain and body weight, brain histology and behavioral outcome in a rat model of IUGR. Twenty-four IUGR-formed rat puppies and 12 healthy puppies were included in the study. IUGR model was established by low (10%) protein diet throughout pregnancy together with intraperitoneal injection of lipopolysaccharide (LPS). Pups were started to be fed with either standard protein (SP), or high protein (HP) diet until postnatal day (PN) 35. Puppies in the control group were given SP diet for 35 days. Six pups from each group were sacrificed at PN7, remaining six were evaluated by Morris water maze test between PN 30 to 35 days and then sacrificed at PN35. Histologic evaluation of brain tissue was performed at PN7 and PN35. IUGR group displayed lower body and brain weights at PN7 when compared with control. At PN35, SP group achieved similar brain/body weight ratios with control, whereas HP group displayed lowest brain/body weight ratio. The number of TUNEL positive cells was significantly higher and myelin basic protein and oligodendrocyte marker O4 immunoreactivity were significantly lower in HP group when compared with SP at PN35. Neuronal density in prefrontal cortex and hippocampus at PN7 were similar among SP and HP groups, but significantly lower in HP group when compared with SP at PN35. SP group displayed better results in the Morris water maze test when compared with HP group. Although postnatal HP support is associated with increase in body weight at PN35, it did not result in better brain/body weight ratios in the rat model of IUGR. In IUGR rats, HP diet was associated with increased apoptosis in brain tissue with lower neuronal density and decreased myelination when compared to SP. Furthermore, better neurodevelopmental scores were achieved by SP diet rather than HP support in IUGR.

Weight gain during the menopause transition: Evidence for a mechanism dependent on protein leverage.

Weight gain during the menopause transition: Evidence for a mechanism dependent on protein leverage.

Characteristics of ruminal microbiota and metabolome in Holstein cows differing in milk protein concentrations.

The rumen is a vital organ containing vast amounts of microbes that play a key role in the digestion of nutrients and affect the production performance of ruminants. However, few studies have focused on the characterization of the ruminal microbiota composition and function in cows with long-term difference milk protein concentrations, and the relationship between milk protein concentration and ruminal microbiota remains elusive. In this study, we collected the data of milk protein concentrations of 1,025 Holstein cows for 10 mo on a commercial farm. Based on the milk protein concentrations, 30 cows were selected and divided into three groups (n = 10 per group): low milk protein group (LMP, milk protein concentration < 3.1%), medium milk protein group (MMP, 3.1% ≤ milk protein concentration < 3.4%), and high milk protein group (HMP, milk protein concentration ≥ 3.4%). The ruminal microbiome, metabolome, VFA concentrations and proportions, and amino acid profiles of the three groups were analyzed. The data showed that free amino acid (FAA) levels were lower in the rumen and higher in the plasma of HMP cows (P < 0.05). In addition, lower NH3 concentrations were observed in the rumen, plasma, and milk of the HMP cows (P < 0.05). Protease activity and isobutyric acid molar proportion in the rumen were lower in the HMP group (P < 0.05). Microbiome analysis showed that HMP cows had lower microbial diversity (represented as Shannon and Simpson indices) than LMP cows. At the genus level, lower relative abundances of Prevotella_1 and Ruminococcaceae_UCG_005 were observed in the HMP group (P < 0.05). At the operational taxonomic unit (OTU) level, a lower relative abundance of OTU3 (Prevotella ruminicola) was observed in the HMP group (P < 0.05). We found that the relative abundances of ruminal Prevotella_1 and OTU3 (Prevotella ruminicola) were negatively correlated with milk protein concentration (P < 0.05). These findings suggested that the cows with long-term high milk protein concentrations had lower microbial diversity and weaker protein degradation ability in the rumen. Furthermore, our observations identified a correlation between the milk protein concentration and ruminal microbiota.

Compromised Hindgut Microbial Digestion, Rather Than Chemical Digestion in the Foregut, Leads to Decreased Nutrient Digestibility in Pigs Fed Low-Protein Diets.

Background: Reduced nutrient digestibility due to low-protein (LP) diets occurring in the foregut or hindgut of pigs remains unclear. Methods: Growing barrows (21.7 ± 1.7 kg) were allotted into LP and high-protein (HP) diet treatments. Ileal digesta and feces were collected for in vitro cross-fermentation and microbial sequencing, and cross-feeding assessed nutrient digestibility. Results: No difference in foregut digesta flora and nutrient digestibility between treatments was observed. LP diet caused decreased total tract digestibility of dry matter (DM), organic matter (OM), gross energy (GE), neutral detergent fiber (NDF), and acid detergent fiber (ADF) compared with the HP diet (p < 0.05). The fermentation broth from LP diet-fed pigs induced less full fermentation digestion of DM, OM, crude protein, and GE than HP broth (p < 0.05). Additionally, LP broth fermentation presented lower fermentation gas and short-chain fatty acids (SCFAs) generation than HP group (p < 0.05). This situation above may be related to decreased abundances of Lachnospiraceae, Eubacterium_eligens_group, Roseburia, and Ruminococcaceae_UCG-009, which can efficiently ferment nutrients to produce SCFA. Conclusions: Change in the flora caused compromise in hindgut microbial fermentation digestion leads to decreased total tract nutrient digestibility in pigs fed an LP diet.

Postmenopausal women with high TNF-α concentrations presented less reduction in fat and blood lipids

Aims: evaluate the effects of a high-protein diet associated with physical exercise on inflammatory markers and body composition. Methods: the study is a 12-week clinical trial of 26 postmenopausal women who received an individualized high-protein food plan and participated in three multicomponent training sessions each week. Food intake was monitored through eight 24-hour food recalls, and the habitual food intake was estimated. At the beginning and end of the study, anthropometric variables were measured; fat content and lean mass were estimated using formulas, and blood was collected for C-reactive protein (CRP) quantification, including TNF-α, IL-6, IL-10, and IL-18. One-way ANOVA was performed.Results: it was identified that 13 participants had a high-protein (HP) diet and 13 had a standard-protein (SP) diet. The HP group lost weight (p = 0.032); however, there were no changes in the fat content, the lean mass content, or the inflammatory markers. Only women who started the program with lower TNF-α values showed significant loss of total fat (p = 0.049), visceral fat (p = 0.037), triglycerides (p = 0.031), and LDL cholesterol (p = 0.003). Conclusion: postmenopausal women with high concentrations of inflammatory markers are less responsive to strategies for modifying body composition.

Effects of Protein Level on the Production and Growth Performance of Juvenile Chinese Mitten Crab (Eriocheir sinensis) and Environmental Parameters in Paddy Fields

Rice–crab co-culture systems represent integrated agriculture–aquaculture systems developed in China over the last 30 years. The rice–crab co-culture area comprised approximately 1.386 × 105 hm2 in 2019. However, there is no specific feed designed for Chinese mitten crab (Eriocheir sinensis) cultured in this system until now. In this study, we investigated feed formulae for the nutritional requirements of Chinese mitten crab in this mode. The control group was not fed with any artificial feed (Co), and the experimental groups were fed with three different feeds of 15% (T15), 30% (T30), or 45% (T45) protein content, respectively. Growth performance variations in E. sinensis were investigated along with water quality, phytoplankton, zooplankton, aquatic vascular plants, and benthic animals in the paddy fields to determine the effect of crabs and their diet on the paddy ecosystem. Dietary protein levels had no significant effect on water quality. The biomass and species of phytoplankton, zooplankton, aquatic vascular plants, and zoobenthos in the paddy field were affected by crabs and their diet. Morphological parameters of crabs were significantly more pronounced in the high-protein group than in the other groups. However, the T45 diet negatively affected production by increasing feed costs, causing precocious puberty and inducing water eutrophication. In conclusion, adding a 15% protein compound feed can meet the nutritional needs of crabs, reduce culture costs, and improve water quality. The discharged water had low ammonia nitrogen and nitrite content and no eutrophication occurred, so the water could be recycled. These findings provide a scientific reference for supporting rice and fish co-cultivation.

EFFECT OF HIGH PROTEIN NORMO-CALORIC NUTRITION ON SKELETAL MUSCLE WASTING ON CRITICALLY ILL MECHANICALLY VENTILATED PATIENTS: A RANDOMIZED, DOUBLE BLIND STUDY

TYPE: Late Breaking Abstract TOPIC: Critical Care PURPOSE: Muscle wasting is a common finding in ICU patients and is associated with poor outcomes. In critically ill patients, ultrasound can reliably track changes in muscle wasting during ICU stay. We sought to measure the impact of high dose protein on muscle thickness and cross-sectional area, as well as muscle echogenicity and pennation angle in critically ill patients. METHODS: We conducted a randomized prospective, double-blind trial. 30 patients anticipated to be intubated for longer than 48 hours with enteral feed were included in the study.Goals in the high protein group were 1.5gm/kg/day with 25kcal/kg/day enteral nutrition compared to standard feed group were 1gm/kg/day with 25 kcal/kg/day enteral nutrition .On days 1, 3,5, and 7 after admission to critical care, muscle thickness, cross-sectional area, and echogenicity, pennation angle was measured in ventilated critically ill patients using bedside ultrasound. Right lower limb Vastus lateralis, medial head of gastrocnemius were investigated. RESULTS: We found progressive loss of muscle mass from day1 to day7 in both the groups. Our study found in the gastrocnemius and vastus lateralis, muscle thickness, and cross-sectional area were significantly high in the HPF group compared to the SF group over 7 days. Muscle echogenicity, pennation angle changes were not significant between the groups during the study period. CONCLUSIONS: In critically ill, mechanically ventilated patients, high dose whey protein decreases the muscle wasting in lower limb weight-bearing muscles in the first seven days of ICU stay. Changes in the qualitative muscle parameters were not significant. CLINICAL IMPLICATIONS: High dose protein diet may prevent skeletal muscle wasting. DISCLOSURE: Nothing to declare. KEYWORD: muscle wasting

Immunomodulating versus high-protein oral preoperative supplement in surgical patients – A two-center, prospective, randomized clinical trial

ObjectivesFor many years, immunonutrition was believed to reduce postoperative complications in patients undergoing major abdominal surgery. However, recent studies questioned that belief. Moreover, the perioperative intake of proteins has gained more and more attention and has shown clinical value. Therefore, the aim of this study was to compare the clinical effect of immunomodulating (IM) plus high-protein (HP) and pure HP supplements during the preoperative period. MethodsBetween January 2011 and December 2020, 299 well-nourished patients (130 female and 169 male; mean age: 60.8 y) undergoing major abdominal surgery at two surgical centers were randomized to receive either preoperative IM or HP oral supplements for 7 d after surgery. In all patients, an enhanced recovery after surgery protocol was applied. Outcome measures of the intend-to-treat analysis were number and type of complications, length of hospitalization, and mortality. ResultsBoth groups were comparable in terms of age, sex, and type of surgery. The median length of postoperative hospital stay was 8 d (range, 6–12 d) in the IM group and 7 d (range, 6–10 d) in the HP group (P = 0.153). Postoperative complications were observed in 29 patients (21.3%) in the IM group and 28 (17.8%) in the HP group (P = 0.442) The risk of readmission was comparable (5.1% vs 4.9%; P = 0.924) for IM and HP supplements, respectively. Postoperative nausea and vomiting occurred in 21 patients in the IM group (15.4%) and 17 patients in the HP group (10.4%; P = 0.195). No difference in gastrointestinal function evaluated with time to first flatus was observed (P = 0.272) ConclusionsThe study demonstrated no difference between preoperative IM + HP and HP supplements in surgical patients. Therefore, the routine preoperative use of IM supplements in all surgical patients cannot be recommended.

Maternal diets have effects on intestinal mucosal flora and susceptibility to colitis of offspring mice during early life

ObjectivesIntestinal flora is considered closely related to the occurrence of inflammatory bowel disease (IBD). This study aimed to discover whether diverse diet conditions during early life lead to different intestinal flora structure and impact different susceptibility to IBD. MethodsWe performed a randomized, controlled trial to investigate the relationship between maternal diet, intestinal flora, and susceptibility of IBD in offspring mice. We treated the maternal mice with different dietary conditions (maternal high fat, high protein, or normal diet, and offspring continued maternal diets or changed to normal diet), and then extracted bacterial meta-genomic DNA from the intestinal mucosa of the offspring during the early life and adult stages. We amplified and sequenced the conserved gene v3-v4 of the bacterial 16 S ribosomal RNA. After dextran sulphate sodium intervention, we evaluated the susceptibility to intestinal inflammation with hematoxylin and eosin stains and disease activity index scores. ResultsThe number of species and alpha diversity of weaning mice (3 wk old) fed a maternal high-protein diet were significantly lower than those of the control diet group (P < 0.05). Among adult (8 wk old) offspring rats, the alpha diversity of mice that continued on a high-protein diet remained significantly decreased (P < 0.05). In addition, 12 kinds of weak bacteria were found in weaning mice fed a maternal high-protein diet compared with the control group. Offspring that continued in the maternal high-protein group had increased disease activity index and pathologic scores after weaning. ConclusionsIn general, our study shows that a maternal high-protein diet during early life can negatively regulate the intestinal flora diversity of offspring mice. A high-protein diet during early life led to higher susceptibility of IBD in offspring rats.

Effects of Parenteral Protein Concentrations in Critically Ill Patients in ICU

Abstract Background: Parenteral protein supplements can prevent deterioration of acute critical illness during admission at intensive care unit (ICU). This study aimed to evaluate the effect of parenteral proteins on ICU outcome and to compare the effect of two different protein concentrations on handgrip strength in critically ill patients. Materials and Methods: This prospective comparative study included 60 acute critically ill patients who had parenteral nutrition during their ICU stay. The patients were divided into two groups: a standard protein group who received protein concentration of 1 g/kg/day (group A) and a high-protein group who received protein concentration of 2 g/kg/day (group B). The nutrition was delivered through a central line and the separate bottles technique. Results: Nitrogen balance was more negative in group B compared to group A within the first 3 days. The handgrip strength on day 7 was significantly higher in group B than group A. The forearm thickness, quadriceps muscle thickness, and overall muscle thickness were significantly higher in group B on day 7. The durations of mechanical ventilation, ICU stay, and total hospital stay were not significantly different between both groups. The protein dose was not significantly associated with the overall 2-month mortality. Conclusion: High parenteral protein intake (2 g/kg/day) associated with better handgrip strength and significant improvement of muscle thickness at the end of the 1st week of follow-up. Studies with larger sample size and longer durations of follow-up are recommended.

Interaction between the Effects of Sustained Swimming Activity and Dietary Macronutrient Proportions on the Redox Status of Gilthead Sea Bream Juveniles (Sparus aurata L.).

The combination of physical exercise and a balanced diet presents substantial health benefits and could improve fish production. However, the redox balance can be affected by training regimen, dietary macronutrient ratio and their interaction. In this study, we conjointly evaluated the effects of physical activity (by voluntary swimming (VS) or sustained swimming as exercise (Ex)) and diet composition (by high-protein (HP) or high-lipid (HE) commercial diets) after 6 weeks on oxidative stress status in liver, white muscle and red muscle of gilthead sea bream juveniles. The HE diet increased the biochemical redox markers’ thiobarbituric acid reactive substances (TBARS), advanced oxidation protein products (AOPP) and reduced thiols (-SH) in the different tissues. Exercise increased AOPP and -SH levels in liver but reduced TBARS levels in white muscle. Regarding the expression of oxidative stress, chaperones and apoptosis-related genes, the VSHE group showed the highest values and the VSHP the lowest, whereas the application of sustained swimming partially equalized those differences. Diet composition modulated the enzyme activity, prioritizing the superoxide dismutase and catalase in the HE-fed groups and the glutathione-related enzymes in the HP groups. Exercise also altered enzyme activity, but in a tissue-dependent manner. Overall, the redox balance in gilthead sea bream juveniles can be affected by diet composition and sustained swimming. However, the response will partly depend on the interaction between these factors and the tissue studied. Therefore, the combination of an adequate diet and sustained exercise could be used in fish production to improve the physiological redox status.

Impact of supplementation with milk–cereal mix during 6–12 months of age on growth at 12 months: a 3-arm randomized controlled trial in Delhi, India

ABSTRACTBackgroundA large proportion of infants in low- and middle-income countries are stunted. These infants are often fed complementary foods that are low-quality, primarily in terms of protein and micronutrients.ObjectivesWe aimed to test 2 milk–cereal mixes supplemented with modest and high amounts of protein during 6–12 mo of age, compared with no supplementation, for their effect on length-for-age z score (LAZ) at 12 mo of age.MethodsEligible infants (6 mo plus ≤29 d) were randomly assigned to either of the 2 interventions (modest- and high-protein) or a no supplement group. The milk–cereal mixes provided ∼125 kcal, 30%–45% energy from fats, and 80%–100% RDA of multiple micronutrients (MMN). The modest-protein group received 2.5 g protein [protein energy ratio (PER): 8%; 0.75 g from milk source] and the high-protein group received 5.6 g protein (PER: 18%, 1.68 g from milk source). One packet was given daily for 180 d. Counseling on continued breastfeeding and optimal infant-care practices was provided to all.ResultsWe enrolled 1548 infants (high-protein: n = 512; modest-protein: n = 519; and no supplement: n = 517). Compared with the no supplement group, there was an improvement in LAZ [adjusted mean difference (MD): 0.08; 95% CI: 0.01, 0.15], weight-for-age z score (MD: 0.12; 95% CI: 0.06, 0.19), weight-for-length z score (MD: 0.11; 95% CI: 0.02, 0.19), and midupper arm circumference z score (MD: 0.10; 95% CI: 0.02, 0.18) in the high-protein group at 12 mo of age. No significant differences for these anthropometric indicators were noted between the modest-protein and no supplement groups or between the high- and modest-protein groups.ConclusionsCereal mixes with higher amounts of milk-based protein and MMN may lead to improvement in linear growth and other anthropometric indexes in infants, compared with no supplementation.This trial was registered at ctri.nic.in as CTRI/2018/04/012932.

Effects of vitamin D and high dairy protein intake on bone mineralization and linear growth in 6- to 8-year-old children: the D-pro randomized trial

Vitamin D and dairy protein may stimulate bone mineralization and linear growth in children, but previous studies show inconsistent results and have not examined their combined effects. To investigate combined and separate effects of vitamin D supplementation and high-protein (HP) compared with normal-protein (NP) yogurt intake on children's bone mineralization and linear growth. In a 2×2-factorial trial, 200 healthy, 6- to 8-year-old, Danish, children with light skin (55°N) were randomized to 20µg/d vitamin D3 or placebo and to substitute 260g/d dairy with HP (10g protein/100g) or NP (3.5g protein/100g) yogurt for 24 weeks during an extended winter. Outcomes were total body less head (TBLH) and lumbar spine bone mineral density (BMD), bone mineral content (BMC), and bone area (BA) by dual-energy X-ray absorptiometry, height, and biomarkers of bone turnover and growth. The primary outcome was TBLH BMD. In total, 184 children (92%) completed the study. The baseline serum 25-hydroxyvitamin D was 80.8±17.2nmol/L, which increased by 7.2±14.1nmol/L and decreased by 32.3±17.5nmol/L with vitamin D and placebo, respectively. The baseline protein intake was 15.4±2.4 energy percentage (E%), which increased to 18.3±3.4 E% with HP. There were no vitamin D-yogurt interactions and no main effects of either intervention on TBLH BMD. However, vitamin D supplementation increased lumbar spine BMD and TBLH BMC compared to placebo, whereas HP groups showed lower increments in lumbar spine BMD, TBLH BMC and BA, and plasma osteocalcin compared to NP groups. Height, growth factors, and parathyroid hormone levels were unaffected. Although there were no effects on whole-body BMD, vitamin D increased bone mass and spinal BMD, whereas high compared with normal dairy protein intake had smaller incremental effects on these outcomes. This supports a recommended vitamin D intake of around 20µg/d during winter but not use of HP dairy products for improved bone mineralization among healthy, well-nourished children. This trial was registered at clinicaltrials.gov as NCT03956732.

Metabolomics Study of Isocaloric Different Dietary Patterns on the Life Span in Healthy Population.

PurposeHow to prolong life by diet has been widely concerned. There are many reports about the effects of different dietary patterns on life span, but the results are not consistent. The main reason may be that total energy intake has not been considered. This study aims to explore the effects of isocaloric different dietary patterns on population life span.Materials and MethodsFrom the data of the follow-up population, eligible participators were divided into normal control (NC) group (28.31% fat, 12.37% protein, 62.30% carbohydrate), isocaloric high-fat (IHF) group (38.39% fat, 12.21% protein, 51.32% carbohydrate), isocaloric high-protein (IHP) group (33.41% fat, 17.10% protein, 52.67% carbohydrate) and isocaloric high-carbohydrate (IHC) group (22.23% fat, 10.52% protein, 70.13% carbohydrate) according to the dietary structure and the age stratification. Global serum metabolic profiling analysis by UPLC−Q-TOF-MS/MS technology, fatty acid and amino acid profiles in serum were determined by GC-MS and UPLC-TQ-MS technology. One-way ANOVA followed by Dunnett post hoc test and receiver operating characteristic (ROC) curve analysis were used to statistical analysis.ResultsNon-targeted metabolomics was to identify 18 potential metabolites related to longevity. ROC curve analysis to identify biomarkers indicated that the areas under the ROC (AUC) of the 12 of 18 biomarkers are above 0.9. The 12 biomarkers were mainly enriched in three metabolic pathways: lipid metabolism, amino acid metabolism and tricarboxylic acid cycle. Compared to control, 11 and 10 of 12 biomarkers showed the same trend with aging in IHP and IHC groups, respectively. Conversely, no differences were observed between IHF group and NC group.ConclusionWithout consideration of the nature of carbohydrates, fats and proteins, IHP and IHC diets might shorten life span by influencing amino acid metabolism, lipid metabolism and tricarboxylic acid cycle metabolism, while the isocaloric IHF diet has no effects on longevity.