High-pressure Homogenization Research Articles

The Fatty Acid Compositions, Irritation Properties, and Potential Applications of Teleogryllus mitratus Oil in Nanoemulsion Development.

This study aimed to characterize and investigate the potential of the oils from Gryllus bimaculatus, Teleogryllus mitratus, and Acheta domesticus to be used in nanoemulsions. The oils were extracted by a cold press method and characterized for their fatty acid profiles. Their irritation effects on the chorioallantoic membrane (CAM) were evaluated, along with investigations of solubility and the required hydrophilic-lipophilic balance (RHLB). Various parameters impacting nanoemulsion generation using high-pressure homogenization were investigated. The findings revealed that G. bimaculatus yielded the highest oil content (24.58% w/w), followed by T. mitratus (20.96% w/w) and A. domesticus (15.46% w/w). Their major fatty acids were palmitic, oleic, and linoleic acids. All oils showed no irritation, suggesting safety for topical use. The RHLB values of each oil were around six-seven. However, they could be successfully developed into nanoemulsions using various surfactants. All cricket oils could be used for the nanoemulsion preparation, but T. mitratus yielded the smallest internal droplet size with acceptable PDI and zeta potential. Nanoemulsion was found to significantly enhance the antioxidant and anti-skin wrinkle of the T. mitratus oil. These findings pointed to the possible use of cricket oils in nanoemulsions, which could be used in various applications, including topical and cosmetic formulations.

Stabilization mechanism of emulsion containing vitamin B12: Impact of preparation methods and surfactant concentration

The aim of this study was to investigate the effect of the preparation methods and the concentration of polyglycerol polyricinoleate (PGPR) on the stability of the water-in-oil (W/O) emulsion. By comparing two different emulsions preparation methods, high-speed shear (16000 rpm) and high-pressure homogenization. We were found that high-speed shear treatment could reduce the particle size of the emulsion more effectively, thus significantly improving the stability of the emulsion. In addition, the effect of PGPR concentration on the stabilization mechanism of the emulsion was also discussed. The results showed that when the concentration of the PGPR was increased from 2 % to 6 %, it effectively prevented emulsion droplets polymerization and improved the stability of the emulsion. When the PGPR concentration was further increased to 10 %, the physical properties of the emulsion were mainly characterized by an increase in viscosity, which further enhanced the stability of the emulsion. In experiments involving the encapsulation of VB12, the encapsulation efficiency (EE) exceeded 95 %, and the retention efficiency (RE) exceeded 85 % even after 21 days of storage, demonstrating excellent stability. This study provided new insights into the preparation of stable water–oil emulsions, particularly regarding the efficient encapsulation and long-term stability of nutrients, and held significant application value for the food and pharmaceutical industries.

Characterization of yeast protein isolates extracted via high-pressure homogenization and pH shift: A promising protein source enriched with essential amino acids and branched-chain amino acids.

In the global food industry, plant-based protein isolates are gaining prominence as an alternative to animal-based counterparts. However, their nutritional value often falters due to insufficient essential amino acids. To address this issue, our study introduces a sustainable protein isolate derived from yeast cells, achieved through high-pressure homogenization (HPH) and alkali pH-shifting treatment. Subjected to HPH pressures ranging from 60 to 120MPa and 1 to 10 cycles, higher pressure and cycle numbers resulted in enhanced disruption of yeast cells. Combining HPH with alkali pH-shifting treatment significantly augmented protein extraction. Four cycles of HPH at 100MPa yielded the optimized protein content, resulting in a yeast protein isolate (YPI) with 75.3g protein per 100g powder, including 30.0g of essential amino acids and 18.4g of branched-chain amino acids per 100g protein. YPI exhibited superior water and oil-holding capacities compared to pea protein isolate, whey protein isolate (WPI), and soy protein isolate. Although YPI exhibited lower emulsifying ability than WPI, it excelled in stabilizing protein-stabilized emulsions. For foaming, YPI outperformed others in both foaming ability and stabilizing protein-based foam. In conclusion, YPI surpasses numerous plant-based protein alternatives in essential amino acids and branched-chain amino acids contents, positioning it as an excellent candidate for widespread utilization as a sustainable protein source in the food industry, owing to its exceptional nutritional advantages, as well as emulsifying and foaming properties. PRACTICAL APPLICATION: This study introduces a sustainable protein isolate derived from yeast cells. YPI exhibited considerable promise as a protein source. Nutritionally, YPI notably surpassed plant-based protein isolates in EAA and BCAA contents. Functionally, YPI demonstrated superior water-holding and oil-holding capacities, as well as an effective emulsion and foam stabilizer.

Improving functional properties of lupin protein by physical modification: high pressure homogenisation

SummaryAqueous dispersions of commercially produced lupin protein isolate with pH 5 and 9 were treated using high pressure homogenisation (HPH) at 25–200 MPa with one to ten homogenisation cycles. Particle size, zeta potential, solubility, molecular weight and rheological properties were analysed. HPH reduced the particle size for lupin protein at pH 5 from 54 to 5 μm and from 14 to <2 μm at pH 9 at 25–100 MPa. However, at 200 MPa, the particle size had less reduction. HPH did not have any significant impact on the zeta potential where it was maintained at −13 mV for pH 5 and −25 mV for pH 9. After HPH, solubility increased significantly for both pH 5 and 9 lupin protein samples. However, prolonged homogenisation decreased solubility. HPH impacted the viscosity of lupin protein at pH 5 and 9, depending on pressure and homogenisation cycles. The highest viscosity (~10 Pa.s) was achieved at 200 MPa with 10 passes. HPH increased the loss modulus where the viscoelastic properties were improved for both pH 5 and 9 at 200 MPa. The storage modulus profile indicated that HPH improved the gelling properties of lupin protein both at pH 5 and 9 at 200 MPa.

Mechanistic understanding of the improvement of the digestive retention of anthocyanins by modified pectin after high-pressure homogenization of strawberry pulp

The physical property and retention of anthocyanins (ACNs) during digestion of strawberry pulp after high-pressure homogenization (HPH) (20/60/100/140 MPa) were analyzed, and their correlations with the three pectin samples—water-soluble (WSP), chelator-soluble (CSP) and sodium carbonate-soluble (NSP)—were evaluated. Compared to the control, the particle size and apparent viscosity of the pulp decreased, and the retention of total ACNs increased, reaching the highest at 100 MPa, with an increase of 15.41%. After HPH treatment, the alcohol-insoluble residue (AIR) and WSP content increased, while the molecular weight (Mw), and microstructural tightness of the three pectin samples decreased. The homogalacturonan (HG) region of WSP increased, and the degree of methylesterification (DM) of CSP decreased. Furthermore, the important role of the modified pectin in the increased retention of ACNs during digestion was reaffirmed by the binding ratio and retention of ACNs in pectin-ACNs complexes. Industrial relevanceThe effect of HPH treatment on the quality attributes of fruits and vegetable juice attracted a lot of attention. This study confirmed that the modification of pectin in HPH-treated strawberry pulp, which promoted its interaction with ACNs, could effectively enhance the retention of ACNs during digestion. Consequently, this study provides the fundamental theory and technological support for the potential to elevate the nutritional value of fruits and vegetables.

Composition, characterisation and emulsifying properties of natural nanoparticles in chickpea aquafaba for the formation of chilli oleoresin-in-water Pickering emulsions

This study aims to assess the compositional characteristics of British Kabuli chickpea aquafaba (AF) by conducting a comprehensive analysis from raw chickpeas to centrifugation fractions of AF and its potential use in forming and stabilising capsaicin-loaded O/W Pickering systems by exploiting the presence of self-assembled nanoparticles and proteins, which act as natural-coating agent. To this end, chickpeas were soaked (16 h, 4 °C, 1:4 chickpea: water) and pressure-cooked (20 min, 113 °C, 10 psig, 1:5 chickpea: water). The dry weight-based (DWB) compositions included total carbohydrate (76.33 ± 4.20%), protein (16.29 ± 0.43%), total phenolics (7.05–8.77 mg/g) and saponins (39.95 ± 0.89 mg/g), thus confirming the leaching of these components from seeds to AF. SDS-PAGE electrophoresis analysis revealed the presence of low MW proteins (≤∼16 kDa). The monosaccharides comprised d-glucose, d-galactose, l-arabinose, d-xylose and d-fructose. AF's particle size distribution revealed the occurrence of a bimodal population of nanoparticles (Dh ≤∼1000 nm and Dh ≤∼100 nm), further characterised by SAXS and TEM imaging. O/W emulsions were prepared with three chilli oleoresin types (capsicum, chilli birds' eye, and chilli ancho) by high-pressure homogenisation. The emulsion with the highest capsaicin content (capsicum oleoresin) was the most stable while the emulsion with the lowest capsaicin content (chilli ancho oleoresin) was the least stable. The presence of incidental nanoparticles and denatured proteins in AF was reasoned to account for the formation and stabilisation of chilli oleoresin-in-water Pickering emulsions, a newly offered explanation for its interfacial properties that will be pursued further in future studies.

Complexation temperature regulated the structure and digestibility of pea starch–gallic acid complexes during high pressure homogenization

Formation of starch–polyphenol complexes by high pressure homogenization (HPH) is widely used to reduce starch digestibility and delay the postprandial glycemic response, thereby benefiting obesity and associated metabolic diseases. This study investigated the effect of complexation temperature on multi–scale structures, physicochemical and digestive properties of pea starch–gallic acid (PS–GA) complexes during HPH process, while also elucidating the corresponding molecular mechanism regulating in vitro digestibility. The results demonstrated that elevating complexation temperature from 30 °C to 100 °C promoted the interaction between PS and GA and reached a peak complex index of 9.22 % at 90 °C through non–covalent binding. The enhanced interaction led to the formation of ordered multi–scale structures within PS–GA complexes, characterized by larger particles that exhibited greater thermal stability and elastic properties. Consequently, the PS–GA complexes exhibited substantially reduced digestion rates with the content of resistant starch increased from 28.50 % to 38.26 %. The potential molecular mechanism underlying how complexation temperature regulated digestibility of PS–GA complexes might be attributed to the synergistic effect of the physical barriers from newly ordered structure and inhibitory effect of GA against digestive enzymes. Overall, our findings contribute to the advancement of current knowledge regarding starch–polyphenol interactions and promote the development of functional starches with low postprandial glycemic responses.

Effect of starch-based emulsion with different amylose content on the gel properties of Nemipterus virgatus surimi

The emulsion was prepared with peanut oil and corn starch with different amylose content using high-speed homogenization assisted high-pressure homogenization, and the effect of starch-based emulsion on the gel properties, whiteness, microstructure, protein secondary structure, chemical forces, texture and sensory properties of Nemipterus virgatus surimi was investigated. The results showed that high amylose corn starch was more beneficial to the stability of emulsion than normal and waxy starch. The gel strength, water holding capacity and texture properties of surimi were significantly improved by adding 10 % waxy corn starch-based emulsion or 15 % high amylose or normal corn starch-based emulsion. Moreover, the whiteness of surimi gel containing starch-based emulsion was higher, and the microstructure was more compact and delicate than that of surimi without emulsion. The addition of starch-based emulsion could increase the hydrophobic interaction and disulfide bond content, and promote the transformation of protein secondary structure to irregular direction. The sensory properties such as color, texture, taste and overall acceptability could be improved to varying degrees. Therefore, starch-based emulsion could be used to enhance the gel properties and nutritional value of surimi products.

Production and Characterization of Cellulose Nanofibrils from Cotton Noil Waste

HighlightsCotton noil is a waste short fiber from the cotton yarn industry.Cellulose nanofibrils were produced by ball-milling and high-pressure homogenization processes.The width of nanofibrils ranged from 50 to 70 nm, with about 80% crystallinity index.The 5% CNF had a tensile strength of about 25 MPa and can be used as a reinforcing material.Abstract. Cellulose nanofibrils (CNF), the nanostructured cellulose, are promising nanomaterials for bio-based barrier coating and packaging applications. In this study, we investigated the production of cellulose nanofibrils from cotton noil, a waste short-fiber from the cotton yarn industry. The cotton noil fibers were subjected to ball milling for 10 min and dispersed in water to prepare slurries with three different concentrations (1%, 5%, and 10% w/v). Then, the slurry was passed through a high-pressure homogenizer at 700 bar pressure for 14 cycles or until the fibers were fibrillated into CNF hydrogels. The CNF with 1% and 5% concentrations had an average width of 70 and 52 nm, respectively, while the 10% CNF concentration had a mix of both nanofibrils and microfibrils due to inconsistent fibrillation. Fourier Transform InfraRed (FTIR) spectroscopy and X-ray diffraction techniques on the isolated CNFs demonstrated that the manufacturing process did not affect the cellulose molecular structure or crystallinity. The tensile strength of CNF from 5% concentration was about 25 MPa. This simple manufacturing method can be applied to utilize cotton noil fibers to produce CNF for various applications. Keywords: Ball milling, Cellulose nanofibrils, Cotton noil, Homogenization, Tensile strength.

Evaluation of the bioprotective potential of gastrointestinal digest fractions of Arthrospira sp. and Nannochloropsis sp. suspensions treated with high pressure homogenisation

Exposure of oxidatively stressed Caco-2 cells to HPH-treated gastrointestinal digests of Arthrospira sp. demonstrating that HPH has potential to enhance the health-promoting properties of selected microalgal species.

Effect of high pressure homogenization on in vitro digestibility and colon fermentability of pea protein-rich bread designed for elderly consumers.

Enrichment of staple foods with proteins can be a solution to tackle protein-energy malnutrition in the elderly. For instance, bread can be enriched with pea proteins that are cheap, sustainable and easily digestible. Non-conventional technologies, such as high pressure homogenization (HPH), can improve the digestibility of plant proteins. To characterize the health functionality of pea-enriched bread, a functional bread tailored to elderly consumers was developed by substituting 5% wheat flour with untreated or HPH-treated pea protein concentrate. Protein digestibility and colon fermentability were assessed by mimicking elderly in vitro gastrointestinal and gut microbiota conditions and compared with adult conditions. Bread reformulation with pea proteins affected physical and chemical properties and produced an increase in hardness, which is one of the key features for the acceptability of bread by the elderly. The highest hardness value was observed for pea protein bread, followed by HPH-treated pea protein bread and wheat bread. In vitro protein digestibility and fermentability were affected by reformulation and by physiological digestive conditions, with lower digestibility under elderly conditions compared to adult ones. The obtained results may contribute to a better understanding of food digestibility under different gastrointestinal conditions and its dependence on physiological and formulation factors, and ultimately would help to design age-tailored foods.

Emulsifying mechanisms of phospholipids in high-pressure homogenization of perfluorocarbon nanoemulsions.

Phospholipids are the most ubiquitous emulsifiers in foods, beverages, pharmaceuticals, and human physiology, but their emulsifying properties are extremely complex. Differential analyses of mechanisms contributing to their functionality are presented in a modular approach. Addition of cholesterol to a natural phospholipid blend disturbs emulsification beyond specific thresholds for size, polydispersity and formation of emulsifying monolayers. Beyond a ratio of lipid concentration to dispersed volume of 1 mM per 1% (v/v) of perfluorocarbon (PFC), phospholipids no longer form monolayers but instead form triple layers that emulsify the PFC. Using synthetic saturated phospholipids, it can be shown that emulsification is most successful for fatty acids closely below their main transition temperature. Phospholipid head groups are more effective for emulsification the more they increase the area per molecule or the zeta potential. Including a comparison with literature results, it can be shown that high molecular weight emulsifiers like proteins are not dependent on the ratio of viscosities η of the dispersed phase to the continuous phase, ηD/ηC. In contrast, smaller molecular weight emulsifiers like phospholipids show a mild increase in effectiveness with rising ηD/ηC, although this increase is not as strong as that observed for low molecular weight detergents. Ruptures of highly resistant emulsifying interfacial layers obviously lead to direct droplet break-up, irrespective of the resistance of a high-viscosity droplet. The lower the break-up resistance of an emulsifier, the more is it governed by the bulk viscosity of the dispersed phase. Our results allow the preparation of a phospholipid-stabilized emulsion with optimized emulsification settings for pharmaceutical applications.

Concentration and desalting of Tetraselmis suecica crude extract by ultrafiltration

Downstream processing, encompassing molecule extraction and extract purification, is a critical step in microalgal biorefinery. This study focuses on the concentration and desalting of Tetraselmis suecica crude extract, which contains proteins, neutral carbohydrates, uronic acids, and pigments. The biomass was initially disrupted with a high-pressure homogenizer operating in moderate conditions (P = 300 bars and 2 passes). The liquid extract obtained was then desalted and concentrated by stirred-cell ultrafiltration. Two membranes, both made of polyethersulfone (PES) but with different molecular weight cut-offs (10 kDa and 30 kDa), were tested for this purpose. The filtration process lasted 3.15 ± 0.34 h, with the temperature maintained at 28 ± 3ºC. There was, therefore, a compelling need to reduce the ash content to facilitate valorization of the extracted proteins. Both membranes displayed time-dependent decreases in permeate flux, membrane permeability, and shear rate. The protein concentration of the permeate increased steadily over time, whereas the concentrations of ash and uronic acids remained constant during ultrafiltration. These results demonstrated the efficacy of both membranes for desalting the extract. After disruption, the ash content in the extract was initially high, at 35.5 ± 0.8% dry weight (DW), decreasing protein purity to 26.1 ± 0.3% DW. The 10 kDa membrane displayed superior molecule retention, resulting in an increase of protein concentration to 50 g.L-1 in the final retentate. The 10 kDa membrane eliminated 79.5 ± 0.5% of salts from the extract, potentially achieving the complete retention of proteins, pigments, and uronic acids; approximately 21.4 ± 3.6% of total carbohydrates were removed by this membrane.

Combination of Indomethacin with Nanostructured Lipid Carriers for Effective Anticancer Therapy.

The anticancer potential of indomethacin and other nonsteroidal anti-inflammatory drugs (NSAIDs) in vitro, in vivo, and in clinical trials is well known and widely reported in the literature, along with their side effects, which are mainly observed in the gastrointestinal tract. Here, we present a strategy for the application of the old drug indomethacin as an anticancer agent by encapsulating it in nanostructured lipid carriers (NLC). We describe the production method of IND-NLC, their physicochemical parameters, and the results of their antiproliferative activity against selected cancer cell lines, which were found to be higher compared to the activity of free indomethacin. IND-NLC were fabricated using the hot high-pressure homogenization method. The nanocarriers were physicochemically characterized, and their biopharmaceutical behaviour and therapeutic efficacy were evaluated in vitro. Lipid nanoparticles IND-NLC exhibited a particle size of 168.1 nm, a negative surface charge (-30.1 mV), low polydispersity index (PDI of 0.139), and high encapsulation efficiency (over 99%). IND-NLC were stable for over 60 days and retained integrity during storage at 4 °C and 25 °C. The potential therapeutic benefits of IND-NLC were screened using in vitro cancer models, where nanocarriers with encapsulated drug effectively inhibited the growth of breast cancer cell line MDA-MB-468 at dosage 15.7 μM. We successfully developed IND-NLC for delivery of indomethacin to cancer cells and confirmed their antitumoral efficacy in in vitro studies. The results suggest that indomethacin encapsulated in lipid nanoparticles possesses high anticancer potential. Moreover, the presented strategy is highly promising and may offer a new alternative for future therapeutic drug innovations.

Rebamipide nanocrystal with improved physicomechanical properties and its assessment through bio-mimicking 3D intestinal permeability model.

This study investigated the formulation and characterization of rebamipide nanocrystals (REB-NCs) to enhance the solubility and permeability of rebamipide, an anti-ulcer medication known for its low aqueous solubility and permeability, classified as BCS class IV. Employing high-pressure homogenization and wet milling techniques, we successfully achieved nanonization of rebamipide, resulting in stable nanosuspensions that were subsequently freeze-dried to produce REB-NCs with an average particle size of 223 nm. Comprehensive characterization techniques, including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), and differential scanning calorimetry (DSC) confirmed the crystalline nature of the nanocrystals and their compatibility with the selected excipients. The saturation solubility study revealed a remarkable three-fold enhancement in PBS pH 7.4 compared to rebamipide API, indicating the effectiveness of the nanocrystal formulation in improving drug solubility. Furthermore, 3D in-vitro permeability assessments conducted on Caco-2 cell monolayers demonstrated an noticeable increase in the permeability of REB-NCs relative to the pure active pharmaceutical ingredient (API), highlighting the promise of this formulation to enhance drug absorption. The dissolution profile of the nanocrystal tablets exhibited immediate release characteristics, significantly outperforming conventional formulations in terms of the dissolution rate. This research underscores the potential of nanomilling as a scalable, environment-friendly, and less toxic approach to significantly enhance the bioavailability of rebamipide. By addressing the challenges associated with the solubility and permeability of poorly water-soluble drugs, our outcome offers insightful information into developing efficient nanomedicine strategies for enhancing therapeutic outcomes.

Evaluation of high-pressure homogenization as a pretreatment for the extraction and drying of 6-gingerol from ginger

6-Gingerol is the major biologically active component found in the ginger rhizome, and this study investigated the effect of high-pressure homogenization pretreatment on the recovery of 6-gingerol.

High-pressure homogenizer valve design modifications allowing intensified drop breakup without increasing power consumption. I. Optimization of current design-principle

This study uses a model-based design approach (CFD coupled to well-established emulsification correlations) to investigate how to choose the valve dimensions of a high-pressure homogenizer so as to achieve intensified drop breakup without increasing the power consumption. Results show how design modifications influence the thermodynamic efficiency of the homogenizer via two effects, a friction factor and a factor related to how high a dissipation rate of turbulent kinetic energy that the design delivers in the centre of the jet. By simple design modifications, thermodynamic efficiency can be increased by 4 % (compared to a representative contemporary valve design) by decreasing the inlet chamber angle, and an additional 5 % by carefully adjusting the outlet chamber angles so as to ensure that the jet exits the gap free of the wall, but then bends and re-attaches to the wall further downstream. The modifications are predicted to allow for achieving the same resulting drop size but with a 17 % lower power draw, compared to a standard contemporary valve, or alternatively a smaller resulting drop diameter at constant power draw.

The preparation, characterization, and antibacterial activity evaluation of nanoliposomes incorporated with terebinth extract

Nanoliposomes are drug release systems that increase bioavailability and are used for encapsulation of therapeutic active ingredients. Terebinth, which is a medicinal plant that grows in many parts of Türkiye, has antibacterial, antioxidant, and anti-inflammatory activity. The antibacterial activity of nanoliposomes incorporated with ethanol extract of terebinth leaves (TLE) was investigated to determine the effects of formulation. The nanoliposome formulation was prepared in two steps which were high pressure and high intensity homogenization techniques. Characterization parameters (zeta potential, particle size and distribution, polydispersity index, and encapsulation efficiency) were determined. After third cycle of microfluidization, the zeta potential charge of nanoliposome dispersion was measured -66.6 mV and 91.13 nm in size. The PDI was 0.231. Also, the nanoliposome encapsulation efficiency was calculated as 91.90%. The TLE was encapsulated with nanoliposomes and their antibacterial activity was examined by disk diffusion and minimum inhibition concentration tests against Escherichia coli ATTC 25922 and Staphylococcus aureus ATTC 25923. Nanoliposome encapsulated TLE (NLTLE) has antibacterial activity against S. aureus ATTC 25923. While NLTLE has less active substance, it displays the same antibacterial activity as TLE.

Effect of High-Pressure Homogenization and Fat Content on Yogurt Fermentation Process

Effect of High-Pressure Homogenization and Fat Content on Yogurt Fermentation Process

Methods for Making a Nanosuspension of Poorly Soluble Medications

Class II prescriptions are known to dissolve ineffectively in both natural and fluid solvents, making them a significantly more challenging challenge. When it comes to these kinds of high log P synthetic compounds that are insoluble in water, the nanosuspension structure is desired. The overall bioavailability of nanosuspensions is influenced by an increase in surface area and a decrease in molecule size. Sometimes the oral dosage forms of water-soluble drugs that are slowly absorbed and inefficient show insufficient bioavailability. A drug's permeability and solubility have a significant impact on how bioavailable it is. To create sub-micron-sized particles, a suitable emulsifier and a pharmaceutical mixture are fed through a high-pressure homogenization or milling procedure. Both classic milling and precipitation processes are commonly used to create particles larger than one millimetre. In this investigation, the techniques was used to prepare the nanosuspension for improving the solubility of poorly soluble drugs.