High Positive Results Research Articles

Integration model of control of lifelong education system in educational clusters and mechanisms of network interaction

Today, in conditions of uncertainty and a high level of competitiveness in the labor market, it is necessary to conduct high-quality training of professional personnel in secondary vocational and higher education in a shorter time. A two-level system of training on one educational platform allows you to maintain the continuity of the content of educational programs. The integration process in the field of education makes it possible to achieve high positive results through the diffusion of differentiated elements and properties into a single professional educational space. The paper considers the main directions of integration processes in the framework of continuing education in the format of educational clusters on the example of a University. This model makes it possible to improve the quality and efficiency of training highly qualified personnel for an intensively developing labor market. The implementation of the integration model of management of the system of continuing education through the mechanism of interaction of educational programs of secondary vocational education and higher education creates a collaborative educational environment, which allows to provide the labor market with qualified personnel in the shortest possible time. A variant of the interaction of the integration model of the functioning of the training of middle-level and higher specialists on the platform of the federal state budgetary educational institution of higher education Samara State Technical University is considered.

Transpersonal exploration of a leader’s transformative journey, and the effects on the organization

This paper explores how the transpersonal approach might bring an actualized view to business and leadership, enabling a collective transformation, giving rise to more engaged, healthier and happier people, reaching high performance and positive business results. This case study examines a real-life experience, investigating the inner transformative journey of a senior business leader, followed by a collective transformation of the company he worked for. An in-depth understanding was formulated through multiple data sources: interviews, associate surveys, reports, office images and meeting protocols. Six themes emerged: preparation, the leaders’ journey of dissolution, the organization’s soul encounter, metamorphosis, enactment and actualization of the senior leader. These themes were explored in relation to the experience of other global business leaders, followed by an exploration of a calling for leadership transformation which aimed to cope with uncertainty and rapid change, and finally, the impact on the organization, when leadership matured from silo and individual-thinking to a common-purpose direction. A description was written to explore the essence of each step of the transformative journey, starting from the senior leader and followed by a collective shift of the organization’s identity. This lived experience shows what can be achieved in an organization when people feel deeply connected with each other and part of a greater purpose, beyond organizational structures.

Deep learning based fetal distress detection from time frequency representation of cardiotocogram signal using Morse wavelet: research study

BackgroundClinically cardiotocography is a technique which is used to monitor and evaluate the level of fetal distress. Even though, CTG is the most widely used device to monitor determine the fetus health, existence of high false positive result from the visual interpretation has a significant contribution to unnecessary surgical delivery or delayed intervention.ObjectiveIn the current study an innovative computer aided fetal distress diagnosing model is developed by using time frequency representation of FHR signal using generalized Morse wavelet and the concept of transfer learning of pre-trained ResNet 50 deep neural network model.MethodFrom the CTG data that is obtained from the only open access CTU-UHB data base only FHR signal is extracted and preprocessed to remove noises and spikes. After preprocessing the time frequency information of FHR signal is extracted by using generalized Morse wavelet and fed to a pre-trained ResNet 50 model which is fine tuned and configured according to the dataset.Main outcome measuresSensitivity (Se), specificity (Sp) and accuracy (Acc) of the model adopted from binary confusion matrix is used as outcome measures.ResultAfter successfully training the model, a comprehensive experimentation of testing is conducted for FHR data for which a recording is made during early stage of labor and last stage of labor. Thus, a promising classification result which is accuracy of 98.7%, sensitivity of 97.0% and specificity 100% are achieved for FHR signal of 1st stage of labor. For FHR recorded in last stage of labor, accuracy of 96.1%, sensitivity of 94.1% and specificity 97.7% are achieved.ConclusionThe developed model can be used as a decision-making aid system for obstetrician and gynecologist.

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Abstract. Introduction. The main factor in ensuring the competitive advantages of the effective functioning of any enterprise, its stable economic development, is the improvement of the efficiency and optimization of the enterprise's costs. Therefore, in order to achieve a high economic and financial result of activity, enterprises of various industries and spheres of operation need effective cost management. Cost management is aimed at the constant search and identification of resource saving reserves, stimulation of resource conservation and cost reduction in order to obtain the effect of commercial calculation based on the results of the enterprise's activities. Purpose. Research of scientific approaches of various scientists to the definition of the concept of �cost management�, determination of cost management functions and systematization of cost management principles at the enterprise. Results. Substantiates the author's position regarding the essence of this concept of �cost management�, which should be considered as an integral system of organizational and functional support and a methodological toolkit of influencing the process of spending, which includes managerial actions , the purpose of which is for the enterprise to obtain a high positive financial result of its activity. It was determined, that cost management at the enterprise involves the performance of general functions inherent in the management of any object and specific functions. It is justified, that consideration of the principles of cost management should be carried out through a thorough coverage of their substantive basis with the disclosure of the opportunities that the enterprise receives in cost management, subject to compliance with one or another principle. The main principles of cost management of the enterprise are summarized, which include the following principles: system-complex approach to cost management; intersubject feedback; resource reservation; continuity of cost management for the production and sale of a specific product; economic validity and reliability of information about costs; the adequacy of the results of management actions regarding costs; independence. Conclusions. The author's position on the essence of cost management is outlined in the article, the general and specific functions of cost management are detailed, and the principles of the cost management process at the enterprise are detailed. It is ensuring maximum compliance of the specified principles at the enterprise that enables the effectiveness of cost management, which is part of the general management system of the enterprise and reflects the efficiency of economic activity.

Теоретичний базис управління витратами підприємства

Abstract. Introduction. The main factor in ensuring the competitive advantages of the effective functioning of any enterprise, its stable economic development, is the improvement of the efficiency and optimization of the enterprise's costs. Therefore, in order to achieve a high economic and financial result of activity, enterprises of various industries and spheres of operation need effective cost management. Cost management is aimed at the constant search and identification of resource saving reserves, stimulation of resource conservation and cost reduction in order to obtain the effect of commercial calculation based on the results of the enterprise's activities. Purpose. Research of scientific approaches of various scientists to the definition of the concept of «cost management», determination of cost management functions and systematization of cost management principles at the enterprise. Results. Substantiates the author's position regarding the essence of this concept of «cost management», which should be considered as an integral system of organizational and functional support and a methodological toolkit of influencing the process of spending, which includes managerial actions , the purpose of which is for the enterprise to obtain a high positive financial result of its activity. It was determined, that cost management at the enterprise involves the performance of general functions inherent in the management of any object and specific functions. It is justified, that consideration of the principles of cost management should be carried out through a thorough coverage of their substantive basis with the disclosure of the opportunities that the enterprise receives in cost management, subject to compliance with one or another principle. The main principles of cost management of the enterprise are summarized, which include the following principles: system-complex approach to cost management; intersubject feedback; resource reservation; continuity of cost management for the production and sale of a specific product; economic validity and reliability of information about costs; the adequacy of the results of management actions regarding costs; independence. Conclusions. The author's position on the essence of cost management is outlined in the article, the general and specific functions of cost management are detailed, and the principles of the cost management process at the enterprise are detailed. It is ensuring maximum compliance of the specified principles at the enterprise that enables the effectiveness of cost management, which is part of the general management system of the enterprise and reflects the efficiency of economic activity.

MIDDLE CEREBRAL ARTERY AND UMBILICAL ARTERY DOPPLER STUDIES TO PREDICT OUTCOME OF PREGNANCIES.

In about 30% of post term pregnancies, the foetuses develop a post maturity syndrome. post term pregnancy is associated with increased risk of both intrauterine and postnatal death. Nowadays, Doppler ultrasound velocimetry of uteroplacental umbilical and fetal vessels has become established method of antenatal monitoring, allowing noninvasive assessment of fetal circulation. Circulatory changes, reected in certain fetal Doppler waveforms, predict adverse perinatal outcome. Umbilical arteries are the common vessels assessed by Doppler ultrasound, but recent studies conrm the efcacy of middle cerebral artery (MCA) Doppler assessment for detecting fetal compromise. Objective- To predict outcome of pregnancies by using umbilical artery and Middle cerebral artery doppler ndings. Methodology- Study group consists of 65 pregnant women with gestational age of 40-42 weeks and control groups include the same number of pregnant women with gestational age of 36-40 weeks. All the antenatal women in the subject & control groups were examined by routine ultrasound scan and Doppler subsequently.The peak systolic, end diastolic, and mean velocity were recorded from these vessels, and Umbilical artery S/D ratio, PI and Middle Cerebral Artery PI and CPR were calculated.Comparison of all variables were done using by·a software package Sensitivity, Specicity, Positive predictive value, Negative predictive value of different arterial Doppler indices and cerebroplacental ratio (CPR) were evaluated. ResultNo statistical signicant difference was noted in UA S/D, UAPI, UARI, MCA S/D, MCAPI MCARI in pregnancy with normal and adverse perinatal outcome. Only CPR showed signicant difference for predicting adverse perinatal outcome (p=0.001). Conclusion- None of the Doppler indices except CPR is sensitive enough. Although CPR cut-off value of 1.335 assures the obstetrician of fetal wellbeing, it's low specicity and high false positive results lead to unnecessary tests, undue concern and unnecessary interference.

Diagnostic Performance of 99mTc-Methoxy-Isobuty-Isonitrile (MIBI) for Risk Stratification of Hypofunctioning Thyroid Nodules: A European Multicenter Study.

99mTc-MIBI (MIBI) imaging is able to exclude malignancy of hypofunctioning thyroid nodules (TNs) with high probability but false positive results are frequent due to low specificity. Therefore, pre-test selection of appropriate TNs is crucial. For image evaluation visual and semiquantitative methods (Washout index, WOInd) are used. Aim of this study was to evaluate the diagnostic performance of MIBI imaging in hypofunctioning TNs with indeterminate fine-needle aspiration cytology results in a multicentric European setting. Patients with hypofunctioning TNs, EU-TIRADS 4 or 5, Bethesda III/IV and MIBI imaging were included. For visual evaluation the intensity of MIBI uptake in the TN was compared to normal thyroid tissue. 358 patients with 365 TNs (n = 68 malignant) were included. Planar imaging (SPECT) showed a sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of 96% (94%), 21% (22%), 22% (15%), 96% (96%), and 35% (32%). The WOInd (38.9% of all cases, optimal cutoff: −19%) showed a sens 100% (spec 89%, PPV 82%, NPV 100%, ACC 93%). For hypofunctioning TNs at intermediate or high risk with indeterminate cytology, a MIBI negative result on visual evaluation is an effective tool to rule-out thyroid malignancy. The semi-quantitative method could considerably improve overall diagnostic performance of MIBI imaging.

Pan-TRK Immunohistochemistry and NTRK Gene Fusions in Primary Carcinomas of the Liver.

Gene fusions involving NTRK are not common in solid tumors. The aim of this study was to investigate the TRK protein expression and molecular characteristics of gene fusions in primary liver carcinomas. A total of 110 hepatocellular carcinomas (HCC) and 69 intrahepatic cholangiocarcinomas were retrieved for tissue microarray (TMA) construction and clinicopathologic characterization. Immunohistochemistry (IHC) for pan-TRK was initially performed on TMA slides and evaluated for staining intensity. Twelve (10.9%) of 110 HCC showed weak cytoplasmic TRK expression by IHC on TMA, while all others, including 69 intrahepatic cholangiocarcinomas, were negative for TRK. The TRK expression did not correlate with patient's age, sex, tumor differentiation, or tumor stage. The 12 cases were then validated by IHC on whole sections but all turned out to be negative. Further, RNA sequencing analysis did not detect any NTRK fusions in all 12 HCC cases; however, it did identify many fusions frequently involving genes that encode mitochondrial and ribosomal proteins, microRNAs, and some transcription factors. A few fusions were recurrent, including MT-ATP6/MT-ATP8 fusion (n=9, 75%), Ig κ light chain gene IGKV/IGKJ fusion (n=5, 41.7%), and histocompatibility complex gene HLA-C/HLA-B fusion (n=4, 33.3%). In summary, NTRK fusion is very rare in primary liver carcinomas. IHC on TMA for TRK expression yields high false positive results, which should be validated on whole sections and confirmed by molecular genetic studies such as RNA sequencing. Many fusions involving genes other than NTRK are detected in HCC, the significance of which warrants further studies.

Development of premature IVF babies with extremely low birth weight: clinical observation (summary review)

Nowadays, when the number of couples affected by infertility increases significantly, the issue of the use of assisted reproductive technologies (ART) is particularly important. In vitro fertilization (IVF) is the ART that is used most frequently due to its high positive results. Priority status initially was accorded by the researchers to quantitative indicators, and the health of children born as a result of ART was analysed extremely rarely. At the present time, all the risks of using ART are being assessed. Despite the success of the procedure, there are times when children are born prematurely. In Russia, starting from 2012 all children born after 24 weeks of gestation and weighing more than 500 grams are legally regarded as human beings and must be provided with life-saving medical care. Observations show that such children have significant health problems: the most serious damage is caused to the nervous system, respiratory organs, and visual system. The article describes cases of premature IVF babies with critically low birth weight and reviews their further development. Approaches to the management of premature IVF babies should be based on the awareness of the problems of extreme morphofunctional immaturity, individual selection of habilitation programs. Observations should be comprehensive (involving narrow specialists according to indications), which plays an important role in the subsequent development of children. If the management of such babies is arranged properly, their health status improves significantly, and physiological parameters achieve close to “normal” standards.

RF AND ANTI-CCPANTIBODY TESTS- A COMPARATIVE STUDY IN THE DIAGNOSIS OF RHEUMATOID ARTHRITIS IN A TERTIARY CARE HOSPITAL

INTRODUCTION: Rheumatoid arthritis (RA) is autoimmune disease associated with chronic inammation of joints causing deformities and functional impairment. Diagnosis primarily depends on clinical manifestations because of lack of suitable diagnostic tests. Rheumatoid factor (RF) is an autoantibody specic for Fc portion of human IgG. RF has low specicity as high false positive results are common in general population. Anti CCP antibody is also useful marker to diagnose rheumatoid arthritis and included in one of the criteria of American College of Rheumatology (ACR) /European League against Rheumatism (EULAR) classication of RA. Thus the present study was planned to compare the diagnostic utility of RF and Anti CCP antibody test in Rheumatoid arthritis patients in a Tertiary Care Hospital. AIM & OBJECTIVE: To compare the diagnostic utility of RF and Anti CCP antibody test in Rheumatoid arthritis patients. Material & METHODS: A total of 200 samples were taken from clinically suspected RA patients over a period of one year.RF was determined by latex agglutination method (SPAN DIAGNOSTICS Ltd.) and Anti CCP antibody by ECLIA (e-Cobas analyzer). The tests were performed as per manufacturer's instructions. RESULTS & DISCUSSION: Out of total 200 samples tested, Both RF and Anti CCP Antibody was positive in 100cases(50%). Only RF positivity was seen in 104(52%) and only Anti CCP antibody was positive in 140 (70%). We found Anti –CCP Sensitivity, Specicity,Positive predictive value,Negative predictive value result as 73.52% , 93.75%, 96.10%, 62.50% respectively.In present study combination of Anti CCPantibody and Rheumatoid factor together have shown positive predictive value for Rheumatoid Arthritis patients which lack specic signs and symptoms related to diagnosis of RA. Conclusion: Anti CCP antibody test and RF can be used concomitantly to diagnose Rheumatoid arthritis and can be used in clinical settings so that appropriate management can be initiated to decrease future morbidity.

Clinical relevance of targeted exome sequencing in patients with rare syndromic short stature

BackgroundLarge-scale genomic analyses have provided insight into the genetic complexity of short stature (SS); however, only a portion of genetic causes have been identified. In this study, we identified disease-causing mutations in a cohort of Korean patients with suspected syndromic SS by targeted exome sequencing (TES).MethodsThirty-four patients in South Korea with suspected syndromic disorders based on abnormal growth and dysmorphic facial features, developmental delay, or accompanying anomalies were enrolled in 2018–2020 and evaluated by TES.ResultsFor 17 of 34 patients with suspected syndromic SS, a genetic diagnosis was obtained by TES. The mean SDS values for height, IGF-1, and IGFBP-3 for these 17 patients were − 3.27 ± 1.25, − 0.42 ± 1.15, and 0.36 ± 1.31, respectively. Most patients displayed distinct facial features (16/17) and developmental delay or intellectual disability (12/17). In 17 patients, 19 genetic variants were identified, including 13 novel heterozygous variants, associated with 15 different genetic diseases, including many inherited rare skeletal disorders and connective tissue diseases (e.g., cleidocranial dysplasia, Hajdu–Cheney syndrome, Sheldon–Hall, acromesomelic dysplasia Maroteaux type, and microcephalic osteodysplastic primordial dwarfism type II). After re-classification by clinical reassessment, including family member testing and segregation studies, 42.1% of variants were pathogenic, 42.1% were likely pathogenic variant, and 15.7% were variants of uncertain significance. Ultra-rare diseases accounted for 12 out of 15 genetic diseases (80%).ConclusionsA high positive result from genetic testing suggests that TES may be an effective diagnostic approach for patients with syndromic SS, with implications for genetic counseling. These results expand the mutation spectrum for rare genetic diseases related to SS in Korea.

Evaluation of Correlation between Serum Free Light-Chain Assay and Bone Marrow Study in Multiple Myeloma Patients.

Background: Multiple myeloma is a hematologic malignancy manifested by the secretion of abnormal immunoglobulin. Different methods have been described for diagnosis and patient response to management. Serum free light-chain assay is recently approved in the diagnosis of multiple myeloma patients. This study aimed to evaluate the diagnostic accuracy of serum free light-chain assay and its agreement to bone marrow findings. Materials and Methods: Forty-six patients with the diagnosis of multiple myeloma were enrolled in the study. The patients were grouped into newly diagnosed cases (22 patients,47.8%) and known cases who were under treatment (24 patients,52.2%). Bone marrow study was done and percentage and clonal status of plasma cells were evaluated by a combination of immunohistochemistry and flow cytometry. Free light-chain assay was done in all patients and sensitivity, specificity, positive predictive value, and negative predictive value were analyzed. Results: Thirty of 46 patients showed monoclonal plasma cell infiltration and 16 patients showed polyclonal plasma cell infiltration based on bone marrow findings. An abnormal κ/λ ratio was seen in 15(68.18%) of new cases and 16(66.6%) of known cases. Sensitivity, specificity, PPV and NPV for κ⁄λ ratio were 72.73%, 46.15%, 71%, and 50%, respectively. Conclusion: In conclusion, due to high false positive and false negative results, the presence of an abnormal serum FLC ratio was not equal to the presence of monoclonal gammopathy, and observation of a normal ratio does not exclude the presence of monoclonal gammopathy.

Bacterial biofilm in adults with ENT pathology

Introduction. Chronic rhinosinusitis, a very common inflammatory condition, is a main public health issue affecting the quality of life. Furthermore, some patients do not respond to either medical or surgical intervention, which could be explained by the presence of the bacterial biofilm in the rhinosinusal zone. Objectives. The aim of this study is to evaluate the influence of endoscopic sinus surgery on olfactory impairment caused by chronic sinusitis with and without nasal polyps (CRSNP and CRS), by testing the olfactory function and potential dysfunction before and after endoscopic sinus surgery. Another objective is to find a connection between the percentage of coverage with bacterial biofilm of the nasal mucosa from patients with CRSNP and CRS and to evidence the fountain of infection role of the bacterial biofilm, while demonstrating that antibiotic therapy is not efficient once the bacterial biofilm is formed in the nasal sinuses. Materials and methods. We investigated 123 patients with CRSNP and CRS, which underwent functional endoscopic sinus surgery (FESS). The olfactory function was tested pre and post FESS. We also analyzed and compared the scores of endoscopic images of Lund-Kennedy and CT staging scale of LundMackey for the two study groups. The degree of olfactory rehabilitation in patients with CRSNP and CRS was evaluated performing smell diskettes test. The presence of bacterial biofilm on the surface of the nasal mucosa extracted during FESS from patients with CRSNP and CRS was examined with the electronic microscope and the percentage of coverage with bacterial biofilm was measured with Carnoy software. Results. Bacterial biofilm was present in a higher percentage in patients with CRS vs. CRSNP. By comparing the level of olfactory function, significant improvement was found after FESS intervention in both study groups. Postoperatively, Lund-Kennedy scores decrease significantly for the whole group (Z = -9.66 at p < 0.001, d Cohen = 4.40), indicating the major role of surgery in the treatment of CRS. The decrease in Lund-Kennedy score values is also significant for each group. In the case of subjects diagnosed with CRS, the mean values decrease from 6.57 (preoperative) to 0.90 (postoperative), respectively Z = -6.779 to p < 0.001. In the case of subjects diagnosed with CRSNP, the mean values decrease from 9.03 (preoperative) to 1.44 (postoperative), Z = -6.927 at p < 0.001. Out of the total number of patients included in the study, 59 patients tested positive with the Prick test for dust and mites and more than half of the patients with positive allergy test were from the lot diagnosed with CRSNP. Conclusions. FESS plays an important role in the improvement of olfactory function in patients with CRS. Bacterial biofilm was present in both study groups but in higher percentage in the CRS group and was found in lower percentage in the group with positive allergy tests. The CRSNP group presented a higher positive result regarding the allergy Prick test and a lower percentage of coverage with bacterial biofilm of the nasal mucosa. In conclusion, chronic rhinosinusitis with or without nasal polyps that is refractary to antibiotic therapy should be directed to the ENT department in order to receive surgical therapy in order to improve olfactory function.

Evaluation of a new interferon gamma release assay, in comparison to tuberculin skin tests and quantiferon tuberculosis goldplus for the detection of latent tuberculosis infection in children from a high tuberculosis burden setting.

: Detection of latent tuberculosis infection (LTBI) in children, exposed to tuberculosis (TB) infections, followed by appropriate treatment, could be instrumental in reducing TB burden. Interferon Gamma release assays (IGRA) have higher specificity than tuberculin skin tests (TST), hence are more effective option for diagnosis. Hence, the present study was aimed to diagnose the presence of latent TB in children with the help of a new Standard E TB-Feron Enzyme-Linked Immunosorbent Assay (TBF) and evaluating its efficiency as compared to TST and Quantiferon TB Gold plus (QFT Plus). Diagnosis of LTBI in participants, <18 years old, who were the close household contacts of patients with active TB was carried out employing techniques such as TST, QFT Plus, and TBF. Higher positive results were obtained with IGRA assays QFT Plus and TBF than TST. The perfect agreement was observed between QFT Plus and TBF assays with a Kappa value of 0.9176 whereas TST and TBF assay showed moderate agreement with a Kappa value of 0.42365. The level of cytokines secreted as a result of stimulation by the antigens from QFT Plus tubes was lower in comparison to antigens from TBF tubes. Hence, TBF, which showed similar efficiency as the widely used QFT Plus, can be a useful detection technique for LTBI in children. Moreover, it could prove to be an efficient alternative to expensive IGRAs like QFT Plus.

CAR-T Therapy, the End of a Chapter or the Beginning of a New One?

Simple SummaryCAR-T therapy is a breakthrough treatment in our fight against cancer. It was recently approved for the treatment of advanced diffuse large B-cell lymphoma and acute lymphoblastic leukaemia after the failure of previous multiple therapies. The positive results achieved in the registration studies for those patients were remarkable. Unfortunately, this was not the end of this chapter. Disease relapses occur in the range of 30–60% of patients treated with CAR-T therapy. Cytokine release syndrome represents a major side effect for treatment with CAR-T therapy. Notwithstanding, the high positive results triggered the start of a huge research activity of CAR-T therapy in other haematologic malignancies such as acute myelogenous leukaemia, Hodgkin’s disease, chronic lymphocytic leukaemia, and multiple myeloma. The research is also trying to overcome the hurdles stated above. These activities represent a new chapter in the management of haematologic malignancies with CAR-T therapy.Chimeric antigen receptor-T (CAR-T) therapy targeting CD19 has revolutionised the treatment of advanced acute lymphoblastic leukaemia (ALL) and diffuse large B-cell lymphoma (DLBCL). The ability to specifically target the cancer cells has shown high positive results as reported in the registration studies. The success of CAR-T therapy in the first two indications led to the initiation of a large number of studies testing CAR-T therapy in different haematologic tumours such as acute myelogenous leukaemia (AML), Hodgkin’s disease (HD), chronic lymphocytic leukaemia (CLL), multiple myeloma (MM), as well as different solid tumours. Unfortunately, relapses occurred in patients treated with CAR-T therapy, calling for the development of effective subsequent therapies. Likewise, this novel mechanism of action was also accompanied by a different toxicity profile, such as cytokine release syndrome (CRS). Patients’ access to the treatment is still limited by its cost. Notwithstanding, this did not prohibit further development of this new therapy to treat other malignancies. This research activity of CAR-T therapy moves it from being used as an end-stage treatment for ALL and DLBCL to a new therapeutic option for a wide range of patients with different haematologic and solid tumours.

Association of Cartridge Based Nucleic Acid Amplification Test (CBNAAT) Grading on the Basis of Cycle Threshold Value with Conventional Microbiological Diagnosis in Pediatric Tuberculosis

Background: Microbiological confirmation of tuberculosis disease in children remains difficult due to paucibacillary disease and inability to obtain optimal samples. Recently introduced Cartridge based nucleic acid amplification test (CBNAAT) has improved microbiological diagnosis in pediatric tuberculosis.&#x0D; Objectives: We aimed to study association of CBNAAT grading based on cycle threshold value with conventional microbiological diagnosis.&#x0D; Methodology: This prospective study was conducted over a period from November 2016 to October 2017 in the Departments of Microbiology and Pediatrics, University College of Medical Sciences and Guru Teg Bahadur Hospital, Delhi. CBNAAT positive pediatric TB cases ≤12 years were recruited and subjected to Ziehl-Neelsen staining for acid fast bacilli (AFB) &amp; culture on Lowenstein Jensen medium. CBNAAT positivity was graded based on cycle threshold value: very low, low, medium and high.&#x0D; Results: Smear and culture positivity was highest (100%) among specimens with high positive CBNAAT result based on CT value. Time to culture positivity was inversely related to CBNAAT grading (p=0.000).&#x0D; Conclusion: CBNAAT grading has significant positive association with smear and culture positivity.&#x0D; Bangladesh Journal of Infectious Diseases 2020;7(2):72-77

Hard disk drive failure prediction using SMART attribute

Disk failures cause data loss and system malfunctions have various severe impacts on the performance and availability of data centers. In data center environments, failures of hard disk drive (HDD) are a very costly & rare occurrence. Therefore, to reduce the rate of failures of this occurrence, different vendors of hard disk as performed a cost saving measure. For the prediction of hard drive failure there are different attributes such as Self-Monitoring and reporting Technology (SMART) collected during normal operations by different HDD manufacturing companies. Now days by using simply threshold values prediction of disk drives failure is done by using SMART attributes. Replacement of healthy disks is very expensive for high false positive rate results. HDD health statistics can be representing by SMART attributes such as the number of command timeout, scan errors and reallocation counts of a Hard disk. The goal of this study is to apply Machine Learning techniques to accurately and proactively predict hard disk drive failures because Machine Learning techniques are best way to solve the learning and rare event-based problem. In evaluating how a method produces the highest accuracy model, we implemented Random Forest algorithms, Decision tree & Naive Bayes algorithm.

An update on joint ill in sheep.

This focus article has been prepared by Vanessa Swinson of the APHA Small Ruminant Expert Group.

Using the Lecturer’s Training Site with a Conscious Approach to Learning Russian as a Foreign Language

The purpose of the research is to demonstrate the productivity of using the lecturer’s training web-site of Russian as a foreign language in the preparatory courses at the University. Lecturer’s training website is an effective means stipulated with principal of consciousness and activity of scholars under the guidance of a lecturer. Training web-site as informative and communicative instrument is able to combine didactic matter, learning techniques and methods for different forms of work organization: full-time, mixed and distance; also, didactic material means educational complexes, information and communication means, control and training. An important principle underlying the proposed teaching method is the refusal to use the methods of unconscious repetition.Materials and methods. To identify the ef fectiveness in application of lecturer’s training web-site various educational platforms were used having different target and national groups. The analysis of studies on mechanical repetitions of well-known physiologists, biologists, lecturers was carried out, which allowed us to come to the conclusion about the ineffectiveness of using techniques with unconscious repetitions in the educational process. Systematic use of lecturer’s web-site in educational process shows that most effective methods and techniques are audio-visual, textual exercises from simple to complex with further audio dubbing, audio method of teaching phonetics and literacy, “lively dialogue” as a new technique in teaching Russian for foreigners, vocabulary works and differentiated approach. Observation, comparison and matching methods were applied, which helped to identify the productivity of learning with the use of multimedia. To identify scientific novelty, an empirical method was used by introducing author’s personal educational web-site project www.russian-teacher.ru with a description of observations and summarizing the results.Results. It is noted that the offered website format is innovation in the field of teaching Russian for foreigners during preparatory course. It was proved with high positive result that the use of a lecturer’s personal training website with the possibility of feedback gives good results when teaching Russian as a foreign language in a short time at the nonlinguistic university during the period of distance learning, with a mixed form of education.Conclusion. Research using the site continues in different groups by nationality (it is possible that there are several linguistic ones), by the number (it is possible to train a group of up to 15 people), by the degree of preparation (possibly A1-B1). The site has a number of advantages: the ability to edit as needed, change the interface and generate content. The study shows how didactics is interconnected with physiology, and the lecturer’s competence includes creativity, which in modern informational conditions is considered through the use of multimedia the lecturer’s training personal website www. russian-teacher.ru.

To determine the efficacy of bilateral inferior petrosal sinus sampling in differentiating Cushing disease from ectopic Cushing syndrome.

Management of endogenous Cushing syndrome is based on its aetiology. Increased Adrenocorticotropic Hormone (ACTH) levels are the most common cause of this disorder and, therefore, it is critical to determine the source of ACTH before further management. Dynamic post contrast MRI is currently the most common investigation implied to diagnose pituitary adenoma; however, it comes with the drawback of low specificity and high false positive results. Inferior petrosal sinus sampling (IPSS) is an established invasive procedure performed to differentiate central versus peripheral source of ACTH which, in turn, results in hypercortesolaemia. This is a series of 14 patients who underwent IPSS at the Department of Radiology, Aga Khan University Hospital, Karachi, from January 2006 to December 2018. The case series emphasises the role of IPSS in the management of ACTH-dependent Cushing syndrome and combined efficacy of Dynamic post-contrast MRI and the procedure under focus.