Short sediment cores (ca. 40 cm long) were collected from three locations within Kootenay Lake, B.C., and the occurrence of diatoms within the cores was recorded and analyzed. This analysis of fossil diatoms in the sediments of Kootenay Lake has provided evidence for environmental change as a result of man's activities in the drainage basin. Changes to the trophic status of the lake are related to operation of a phosphate fertilizer plant (beginning in 1953) on the major southern inlet river and to more recent water regulation on both the southern and northern major inlet rivers. Diatom numbers and volumes at the deeper core depths were lowest. Diatom species in the deeper core depths consisted of an assemblage of oligotrophic and eutrophic indicators corresponding to high (but not critically high) phosphorus loading to the lake. Above the 5-cm depth, numbers increased and peaked at the 3-cm depth in samples nearest the south end of the lake. At other locations within the lake, where sedimentation rates were lower, numbers peaked in the top centimetre of sediment. Concomitant with increased phosphorus loading diatom species indicative of oligotrophic conditions such as Cyclotella ocellata declined, while eutrophic indicators including Cyclotella glomerata, Melosira granulata v. angustissima, Stephanodiscus spp., Asterionella formosa, and Fragilaria crotonensis increased. Asterionella formosa and Fragilaria crotonensis showed highest abundances in the top 2 cm of the core. These species, which form a major component of the spring and fall diatom blooms, were responding to both increased nutrients and improved light conditions resulting from decreased freshet turbidity. The Araphidineae/Centrales ratio was not useful in classifying trophic changes in Kootenay Lake since numbers of centric diatoms did not decline with nutrient enrichments (although there was a dominance shift from oligotrophic centrics to eutrophic centrics). Shannon–Wiener diversity values remained high throughout the recent history of the lake.