High-value Food Research Articles

Biotransformation of insect processing residues: Production of lactic acid bacterial biomass and associated partial removal of proteins from chitin

Processing of edible insects typically involves fractionating into high-value food ingredients, which results in by-products containing chitin and insoluble proteins. This study examined the effectiveness of lactic acid bacteria (LAB) in removing proteins from chitin in insect processing residues. Lesser mealworm processing residues were biologically treated for 48 and 120 h using LAB strains without added carbon sources. Results showed partial deproteinization, up to 29 % with Levilactobacillus brevis after 120 h. Most LAB grew up to 2 log10 colony-forming units/mL in the first 48 h. Confocal microscopy and Fourier-transform infrared spectra indicated that some protein remained attached to chitin. The molecular weight of solubilized proteins was affected by strain and time of incubation, with antioxidant activity increasing significantly after 120 h with Lacticaseibacillus paracasei. The biological treatment of insect processing streams can be a sustainable approach to producing high amounts of LAB biomass with subsequent protein solubilization and chitin release.

Enrichment of deoxynojirimycin in mulberry using cation exchange resin: Adsorption/desorption characteristics and process optimization

Deoxynojirimycin (DNJ) is an α-glucosidase inhibitor with high food values. However, the complex and costly enrichment processes have greatly prevented its application. Herein, this study aimed to propose a simple and efficient enrichment process for DNJ from Morus alba L. extracts using cation exchange resins. The LSI and D113 resins were chosen due to their excellent adsorption and desorption properties. The adsorption characteristics agreed with the pseudo-first-order kinetic model and the Langmuir isotherm model. This adsorption was chemisorption, spontaneous, endothermic and entropy-driven. Furthermore, the concentration and pH of the extracts, desorption solvent, breakthrough and elution curves, sample loading and elution rate were investigated to optimize the enrichment process by resin column chromatography. The results also showed that the purity of DNJ was improved to 44.00 % with a total recovery of 78.21 % using the LSI-D113 combination strategy. This research demonstrated the industrial feasibility of DNJ enrichment using cation exchange resins.

Determination of Proximate Content and Phytochemical Analysis of Some Selected Grains, Fruits and Vegetables Commercially Sold in Eke Awka Market, Awka, Anambra State, Nigeria

Six samples from cucumber (Cucumis sativus), apple (Malus domestica), cabbage (Brassica oleracea), Tigernuts (Cyperus esculentus), Beans (Phaseolus vulgaris), Green peas (Pisum sativum) were purchased from Eke Awka market, Awka in Anambra state, and evaluated for their proximate composition and phytochemicals content using the standard procedures as described by Association of Official Analytical Collaboration International, (1995). The study revealed that moisture content of cucumber was significantly high, followed by cabbage, apple and then tiger nuts. The outcome of Phytochemical analysis showed that Alkaloids, Flavonoid Phenol and Oxalate levels were highest in cabbage; Saponin and Tannin were highest in tiger nuts while Steroids and Phytate were highest in cucumber. The fruits and vegetables analyzed contain appreciable amounts of phytochemicals and relatively high food value thus making them valuable nutraceutical sources and very useful for nutritional and medicinal purposes Keywords: Proximate composition, Phytochemical analysis, Grains, Fruits, Vegetables

The emulsifying capacity and stability of potato proteins and peptides: A comprehensive review.

The potato has recently attracted more attention as a promising protein source. Potato proteins are commonly extracted from potato fruit juice, a byproduct of starch production. Potato proteins are characterized by superior techno-functional properties, such as water solubility, gel-forming, emulsifying, and foaming properties. However, commercially isolated potato proteins are often denatured, leading to a loss of these functionalities. Extensive research has explored the influence of different conditions and techniques on the emulsifying capacity and stability of potato proteins. However, there has been no comprehensive review of this topic yet. This paper aims to provide an in-depth overview of current research progress on the emulsifying capacity and stability of potato proteins and peptides, discussing research challenges and future perspectives. This paper discusses genetic diversity in potato proteins and various methods for extracting proteins from potatoes, including thermal and acid precipitation, salt precipitation, organic solvent precipitation, carboxymethyl cellulose complexation, chromatography, and membrane technology. It also covers enzymatic hydrolysis for producing potato-derived peptides and methods for identifying potato protein-derived emulsifying peptides. Furthermore, it reviews the influence of factors, such as physicochemical properties, environmental conditions, and food-processing techniques on the emulsifying capacity and stability of potato proteins and their derived peptides. Finally, it highlights chemical modifications, such as acylation, succinylation, phosphorylation, and glycation to enhance emulsifying capacity and stability. This review provides insight into future research directions for utilizing potato proteins as sustainable protein sources and high-value food emulsifiers, thereby contributing to adding value to the potato processing industry.

Exploitation and Application of a New Genetic Sex Marker Based on Intron Insertion Variation of erc2 Gene in Oplegnathus punctatus.

Spotted knifejaw (Oplegnathus punctatus), one of the most valuable mariculture species, grows with significant sexual dimorphism, with males growing significantly faster than females. O. punctatus not only has excellent growth characteristics and high food value, but also shows high economic value in aquaculture, which has become a hotspot in the field of aquaculture. The current insufficiency of sex marker identification in O. punctatus restricts the process of its unisexual breeding. Rapid identification of sex will help to study the mechanisms of sex determination and accelerate the development of sex-controlled breeding. With the completion of the sequencing of the male and female genomes of O. punctatus, the efficient and precise development of genetic sex markers has been made possible. In this study, we used genome-wide information combined with molecular biology techniques from marker sequences to further establish a rapid method for DNA insertion variant detection in the intron of O. punctatus erc2 gene, which can be used to rapidly, accurately, and efficiently identify whether DNA insertion occurs in the intron of O. punctatus erc2 gene to be detected, and to identify the sex of O. punctatus to be detected. It could also be distinguished by agarose gel electrophoresis, which would shorten the time for accurate identification and improves the detection efficiency. Homozygous comparison of male and female individuals showed that the length of the DNA fragment of the erc2 gene was 239bp on chromosome X1 and 1173bp on chromosome Y. It can therefore be inferred that a 934bp insertion fragment exists on the Y chromosome. The PCR amplification results showed that two DNA fragments of 1173bp and 239bp could be amplified in male O. punctatus, and the 1173bp fragment was a marker fragment specific to the variant intron erc2 gene, while only a single DNA fragment of 239bp was amplified in female O. punctatus. It has important significance and application value in the study of neurotransmitter transmission and environmental adaptability of female and male fish based on erc2 gene, as well as the identification of male and female sex, the preparation of high male fry, and family breeding.

Development of DNA Insertion-specific Markers Based on the Intergenic Region of Oplegnathus punctatus Cdkn1/srsf3 for Sex Identification.

Spotted knifejaw (Oplegnathus punctatus) is a marine economic fish with high food and ecological value, and its growth process has obvious male and female sexual dimorphism, with males growing significantly faster than females. However, the current sex identification technology is not yet mature, which will limit the growth rate of O. punctatus aquaculture and the efficiency of separate sex breeding, so the development of efficient sex molecular markers is imperative. This study identified a 926bp DNA insertion fragment in the cdkn1/srsf3 intergenic region of O. punctatus males through whole-genome scanning, comparative genomics, and structural variant analysis. A pair of primers was designed based on the insertion information of the Y chromosome intergenic region in male individuals. Agarose gel electrophoresis revealed the amplification of two DNA fragments, 1118bp and 192bp, in male O. punctatus individuals. The 926bp fragment was identified as the insertion in the intergenic region of cdkn1/srsf3 in males, while only a single 192bp DNA fragment was amplified in females. The biological sex of the individuals identified in this manner was consistent with their known phenotypic sex.In this study, we developed a method to detect DNA insertion variants in the intergenic region of O. punctatus. Additionally, we introduced a new DNA marker for the rapid identification of the sex of O. punctatus, which enhances detection efficiency. The text has important reference significance and application value in sex identification, all-male breeding, and lineage selection. It provides new insights into the regulation of variation in the intergenic region of cdkn1/srsf3 genes and the study of RNA shearing.

Effects of High Hydrostatic Pressure on the Distribution of Oligosaccharides, Pinitol, Soysapapogenol A, and Fatty Acids in Soybean.

The effects of high hydrostatic pressure (HHP) treatment (100-600 MPa for 10-60 min) and thermal treatment (boiling for 10-60 min) on oligosaccharides, pinitol, and soyasapogenol A as taste ingredients in soybean (Glycine max (L.) Merr.) (cv. Yukihomare) were evaluated. Additionally, soybean-derived fatty acids such as α-linolenic acid, linoleic acid, oleic acid, palmitic acid, and stearic acid in pressurized soybeans were quantitatively analyzed. Sucrose, stachyose, and raffinose concentrations were decreased in all tested pressure and time combinations; however, pinitol concentrations were increased by specific pressure and time combinations at 100-400 MPa for 10-60 min. While the soyasapogenol A content in boiled soybeans decreased with increasing boiling time, that of pressurized soybeans was altered by specific pressure and time combinations. At the lower pressure and shorter time combinations, the essential fatty acids such as α-linolenic acid and linoleic acid showed higher contents. Stearic acid and oleic acid contents of pressurized soybeans increased at mild pressure levels (300-500 MPa). In contrast, the combination of higher pressure and longer time results in lower essential fatty acid contents. Non-thermal-pressurized soybeans have the potential to be a high-value food source with better taste due to the enrichment of low molecular weight components such as pinitol, free amino acids, and the reduction of isoflavones and Group A soyasapogenol.

КРАСНИКА (VACCINIUM PRAESTANS LAMB.): РАЗРАБОТКА МЕТОДИКИ ПРОВЕДЕНИЯ ИСПЫТАНИЙ НА ОТЛИЧИМОСТЬ, ОДНОРОДНОСТЬ И СТАБИЛЬНОСТЬ

The purpose of research is to study the morphological and phenological characteristics of hybrid forms of V. praestans and to develop a methodology for testing for distinctiveness, homogeneity and stability based on them. The results of studying the morphological and phenological characteristics of hybrid forms of red grass (Vaccinium praestans Lamb.) in the natural and climatic conditions of the European part of Russia (Moscow, Kostroma, Khabarovsk Regions) are presented. V. praestans is an endemic plant of the Russian Far East and has high medicinal, food and ornamental value. The experiments carried out on the introduction of V. praestans show the possibility of its successful cultivation in the conditions of the European part of Russia, including for industrial cultivation. We tested 2 hybrid forms obtained from specimens from natural habitats (Sakhalin, Khabarovsk Regions). The current year's shoots of V. praestans are long or medium in size and have a pronounced anthocyanin color; leaves are large and medium in size, with a low or medium length-to-width ratio, with an ovate or broadly ovate shape of the leaf blade, with a weak or medium expression of anthocyanin coloring, sparse or medium pubescence of the leaf edge; inflorescences are short or medium in length, with large or medium-sized flowers, pink or slightly pink in color; fruits are spherical berries, large or medium in size, glossy, fairly dense, red or dark red in color. Peculiarities of flowering and fruiting of V. praestans have been revealed. The periods of the beginning of flowering and the beginning of fruit ripening are early or middle. For the first time in Russia and in the world, based on an analysis of the morphological characteristics of shoots, leaves, flowers and fruits, a methodology for testing the distinctiveness, homogeneity and stability of hybrid forms and varieties of V. praestans has been developed.

Analysis of Economic Efficiency: Case of Sweet Potato Seed Vine Production in Homabay County, Kenya

Sweet potatoes are not only a nutritious, high value food for the daily diet, but also an important source of income. In the selected survey areas in the Lake Region of Kenya, sweet potato is amongst the major commercially grown crops. Therefore, in order to understand the profitability and performance of sweet potato producers in the study area, this study was implemented. Sweet potato is one of the main commercially produced crops in the selected survey areas in the lake region of Kenya. Therefore, this study was conducted to understand the profitability and performance of sweet potato producers in the study areas. The study employed Cost Function in the analyses of Economic Efficiency of Sweet Potato Seed Vine Production system in Kenya. A multi-stage random sampling technique was used to select sample size of 150 sweet potato vine producers for this study. By use of a structured questionnaire, data was collected on production input, output, input and output prices, characteristics of sampled farmer and plot. Using the maximum likelihood method, the variables of the cost function were estimated. Result revealed that the farm level cost- efficiency was about 56 percent. The research has shown that age and farm size were negative but significantly related to cost-efficiency at ten and one percent respectively. Gender, farming experience, Education, access extension to and credit were positive but significantly associated with cost-efficiency at one percent. The study advocates for policy decision that youthful farmers be inspired to venture into SP vine production and the government to enhance access to education, credit and extension services the ongoing farming.

Terahertz time-domain spectroscopy imaging combined with convolutional long short-term memory neural network for In situ moisture reduction prediction of particle foods during pulsed fluidized bed drying

Fluidized bed drying (FBD), as a novel dehydration method, has been widely applied in drying various heat-sensitive high-value food products, in which the pulsed fluidized bed drying (PFBD) technique addresses the ineffective fluidized limitation of the traditional FBD when dealing with cohesive products. However, achieving in situ real-time monitoring of dynamic product moisture content (MC) change during both FBD and PFBD without sampling is still challenging. This paper presents an innovative integration of a whisk-broom terahertz time-domain spectroscopy (THz-TDS) imaging system with a fluidized bed dryer, facilitating in situ, real-time monitoring of MC reduction of products without process pausing for sampling. The reliability, and robustness of the established system and prediction models were tested by drying peppercorn and sweetcorn under various drying settings, including different temperatures, flow patterns, and durations. Implementing the convolutional neural networks (CNN) and long short-term memory (LSTM) for correlating terahertz time-domain image data with reference datasets in this research provided reliable MC reduction prediction results for the mixture dataset from peppercorn and sweetcorn drying. The successful application of this combined sensing and analytical method presents a promising approach for enhancing industrial processing control and expands the utility of THz-TDS in the agriculture and food industrial fields.

Parataxonomy and diversity of local varieties of taro (Colocasia esculenta (L.) Schott) and macabo (Xanthosoma sagittifolium (L.) Schott) grown in Benin (West Africa)

Taro and macabo are plants with high food, medicinal and economic value, but are under-utilized in Benin. The aim of this study was to identify local varieties of taro and macabo grown in Benin, with their vernacular names, farmers' recognition criteria and distribution. Data were collected between May 2021 and May 2023, through interviews using semi-structured questionnaires. The sample surveyed consisted of 349 taro/macabo producers, distributed in 40 villages belonging to 9 departments. The variables collected concerned producers' socio-demographic characteristics, local appellations, farmers' recognition criteria and the distribution of local varieties of the two species, and were processed by calculating relative citation frequencies and establishing a generalized linear model in R software. In addition, QGIS software was used to represent the distribution of the varieties surveyed. A total of 42 local appellations have been registered to designate all local varieties. Ten local varieties, including six of C. esculenta (V1 to V6) and four of X. sagittifolium (V7 to V10), were identified on the basis of seven (07) farmers' criteria, the main ones being tuber color (41.88 %) and leaf blade color (23.30 %). Local varieties V1, V3, V4 and V5 were the most widespread for C. esculenta, while varieties V7 and V9 were the most widely distributed from the south to the north of the country for X. sagittifolium. The socio-demographic parameters such as age category, occupation and ethnic groups tested had a significant influence (p < 0.05) on the number of local varieties held by growers. Agro-morphological characterization will provide data for the selection of elite varieties.

Brown-top millet: an overview of breeding, genetic, and genomic resources development for crop improvement.

Brown-top millet is a lesser-known millet with a high grain nutrient value, early maturation, and drought tolerance that needs basic research to understand and conserve food security. Brown-top millet [Urochloa ramosa (L.)] is currently cultivated in some developing countries (especially in India) for food and fodder, although it is less known among the small millets. Like other millets, it contains macro- and micronutrients, vitamins, minerals, proteins, and fiber, all of which have rich health benefits. The nutritional importance and health benefits of brown-top millet are still unknown to many people due to a lack of awareness, wide cultivation, and research. Hence, this millet is currently overshadowed by other major cereals. This review article aims to present the nutritional, breeding, genetic, and genomic resources of brown-top millet to inform millet and other plant researchers. It is important to note that genetic and genomic resources have not yet been created for this millet. To date, there are no genomic and transcriptomic resources for brown-top millet to develop single nucleotide polymorphisms (SNP) and insertion/Deletions (InDels) for breeding studies. Furthermore, studies regarding nutritional significance and health benefits are required to investigate the exact nutritional contents and health benefits of the brown-top millet. The present review delves into the nutritional value and health advantages of brown-top millet, as supported by the available literature. The limitations of producing brown-top millet have been enumerated. We also cover the status of marker-assisted breeding and functional genomics research on closely related species. Lastly, we draw insights for further research such as developing omics resources and applying genome editing to study and improve brown-top millet. This review will help to start breeding and other molecular studies to increase the growth and development of this cereal.

Classification of eco-zones from the factors and processes controlling phytoplankton biomass

Phytoplankton is of utmost importance to the marine ecosystem and, subsequently, to the Blue Economy. This study aims to explain the reasons for variability of phytoplankton by estimating the dependency of Chlorophyll-a (Chl-a) on various limiting factors using statistics. The global oceans are classified into coherent units that display similar sensitivity to changing parameters and processes using the k-means algorithm. The resulting six clusters are based on the limiting factors (PAR, iron, or nitrate) that modulate Chl-a yield divisions of the oceans, similar to regions of different trophic statuses. The clusters range from the polar and equatorial regions with high nutrient values limited by light, to open oceanic regions in downwelling gyres limited by nutrients. Some clusters also show a high dependency on marine dissolved iron. Further, oceans are also divided into eight clusters based on the processes (stratification, upwelling, topography, and solar insolation) that impact ocean productivity. The study shows that considering temporal variations is crucial for segregating oceans into ecological zones by utilizing correlation of time-series data into classification. Our results provide valuable insights into the regulation of phytoplankton abundance and its variability, which can help in understanding the implications of climate change and other anthropogenic effects on marine biology.

Volatile Profiling of Spirulina Food Supplements.

Spirulina, a cyanobacterium widely used as a food supplement due to its high nutrient value, contains volatile organic compounds (VOCs). It is crucial to assess the presence of VOCs in commercial spirulina products, as they could influence sensory quality, various processes, and technological aspects. In this study, the volatile profiles of seventeen commercial spirulina food supplements were determined using headspace solid-phase microextraction (HS-SPME), coupled with gas chromatography-mass spectrometry (GC-MS). The identification of volatile compounds was achieved using a workflow that combined data processing with software tools and reference databases, as well as retention indices (RI) and elution order data. A total of 128 VOCs were identified as belonging to chemical groups of alkanes (47.2%), ketones (25.7%), aldehydes (10.9%), alcohols (8.4%), furans (3.7%), alkenes (1.8%), esters (1.1%), pyrazines (0.8%), and other compounds (0.4%). Major volatiles among all samples were hydrocarbons, especially heptadecane and heptadec-8-ene, followed by ketones (i.e., 4-(2,6,6-trimethyl-1-cyclohexen-1-yl)-3-buten-2-one, β-ionone, 2,2,6-trimethylcyclohexan-1-one), aldehydes (i.e., hexanal), and the alcohol oct-1-en-3-ol. Several volatiles were found in spirulina dietary supplements for the first time, including 6,10-dimethylundeca-5,9-dien-2-one (geranylacetone), 6,10,14-trimethylpentadecan-2-one, hept-2-enal, octanal, nonanal, oct-2-en-1-ol, heptan-1-ol, nonan-1-ol, tetradec-9-en-1-ol, 4,4-dimethylcyclohex-2-en-1-ol, 2,6-diethylpyrazine, and 1-(2,5-dimethylfuran-3-yl) ethanone. The methodology used for VOC analysis ensured high accuracy, reliability, and confidence in compound identification. Results reveal a wide variety of volatiles in commercial spirulina products, with numerous newly discovered compounds, prompting further research on sensory quality and production methods.

Argemone ochroleuca Phytochemicals and Allelopathic Effect of Their Extracts on Germination of Soybean

Soybean is a high-value food source, and the invasive weeds Mexican prickly poppy (Argemone ochroleuca) could release allelochemicals that inhibit the growth of this crop. The impact of A. ochroleuca on the germination and growth of soybean is not well documented. Therefore, the aim of this study was to evaluate the TLC profiles of different extracts of A. ochroleuca and assess the effects of extracts on the germination of soybean seeds. Shoots and roots of A. ochroleuca were weighed and 100 g of each was separately extracted with 1000 mL deionized water, hexane or acetone. Ten concentrations of water extracts ranging from 10 to 100 mL per 100 mL of deionized water and three concentrations of acetone and hexane extracts ranging from 2.5 to 7.5 g/L were separately used for seed germination bioassays. Thin-layer chromatography (TLC) analysis was used to compare the chemical profiles in the shoot and root water, and in the hexane and acetone extracts of A. ochroleuca. The highest reduction was recorded from the water extract, at 100%. The TLC profiling of A. ochroleuca addressed different classes of compounds, including alkaloids, phenolic acids and flavanoids. There is, however, a need to identify the most active phytochemicals in the suppression of germination.

Peanut Smut in Argentina: An Analysis of the Disease, Advances, and Challenges.

Peanut (Arachis hypogaea L.) is a globally high-value food crop, with Argentina ranking third in global peanut exports. However, Argentine peanut production faces a severe threat from a fungal disease, peanut smut, caused by Thecaphora frezzii. This disease is particularly prevalent in the Córdoba Province, where recent surveys have documented a gradual increase in the prevalence and incidence of peanut smut, becoming a significant challenge to peanut production. First identified in Brazil in the 1960s in wild peanut and later in Argentina in 1995 in commercial peanut fields, the disease has rapidly spread owing to its distinctive pathogen characteristics, including the lack of visible symptoms on aerial plant parts, spore spread, and survival, and with a lack of proactive efforts to develop and apply management strategies. This results in the gradual accumulation of teliospores of T. frezzii in soil, further exacerbating the problem in subsequent growing seasons by increasing the intensity of the disease and driving a reduction in crop yield and quality. This review summarizes recent research on peanut smut, focusing on disease assessment, molecular characterization, diagnosis and detection, epidemiology, host range and environmental conditions, and the latest advancements in management approaches, including fungicide spraying, breeding programs, cultural management, and biological control, aimed to enhance understanding and support effective disease management strategies in peanut production systems.

Therapeutic Effects of Amaranth: Analysis of the Antidiabetic Potential of the Plant.

Amaranth is a pseudocereal rich in macronutrients and micronutrients, with about 60 species cultivated worldwide. It is a high nutritional value food because of its many essential amino acids. Recent investigations demonstrate that the phytochemicals and extracts of amaranth have beneficial effects on health, including antidiabetic potential, a decrease in plasmatic cholesterol and blood pressure, and protection from oxidative stress and inflammation. Nowadays, type 2 diabetes has increased worldwide, becoming a problem of public health that makes it necessary to look for alternative strategies for its prevention and treatment. This review aims to summarize the antidiabetic potential of diverse species of the Amaranth genus. A bibliographical review was updated on the plant's therapeutic potential, including stem, leaves, and seeds, to know the benefits and potential as an adjuvant in treating and managing diabetes and associated pathologies (hypertension, dyslipidemia, and heart disease). This analysis contributes to the generation of knowledge about the therapeutic effects of amaranth, promoting the creation of new products, and the opportunity to conduct clinical trials to assess their safety and efficacy.

Green and efficient method to acquire high-value phycobiliprotein from microalgal biomass involving deep eutectic solvent-based ultrasound-assisted extraction

Phycoerythrin (PE) is a phycobiliprotein holding great potential as a high-value food colorant and medicine. Deep eutectic solvent (DES)-based ultrasound-assisted extraction (UAE) was applied to extract B-PE by disrupting the resistant polysaccharide cell wall of Porphyridium purpureum. The solubility of cell wall monomers in 31 DESs was predicted using COSMO-RS. Five glycerol-based DESs were tested for extraction, all of which showed significantly higher B-PE yields by up to 13.5 folds than water. The DES-dependent B-PE extraction efficiencies were proposedly associated with different cell disrupting capabilities and protein stabilizing effects of DESs. The DES-based UAE method could be considered green according to a metric assessment tool, AGREEprep. The crude extract containing DES was further subjected to aqueous two-phase system, two-step ammonium sulfate precipitation, and ultrafiltration processes. The final purified B-PE had a PE purity ratio of 3.60 and a PC purity ratio of 0.08, comparable to the purity of commercial products.

Production of techno-functional proteins and plant biostimulants from Nannochloropsis gaditana

Nannochloropsis gaditana is produced mainly for its lipid content and fatty acid profile. However, its protein content is comparable to or even higher than that of most conventional protein sources. In this work, the microalga N. gaditana BEA 1202B purchased from the Spanish Bank of Algae (BEA) was produced using 3.4 m3 tubular photobioreactors achieving a biomass productivity of 0.1 g L−1·day−1 and a protein content of 29.2 ± 4.5 % (d.w.). The biomass produced was used as a source of proteins; the protein recovery was optimised following a response surface methodology. Under optimal conditions, which were solubilisation at pH = 10.2 and precipitation at pH = 3.1, the protein recovery yield was 17.5 g·100 g−1. The extract exhibited a water-holding and an oil-holding capacity of 2.1 and 3.0 g g−1, respectively. The leftovers from protein extraction were assessed as plant biostimulants and demonstrated a positive effect on root development, weight gain, and chlorophyll retention. The process described in this work presents a sustainable and environmentally friendly approach to harness the potential of the microalga N. gaditana in producing high-value food proteins and plant biostimulants. The integration of this microalgal-based biorefinery concept could contribute to more sustainable agriculture and address the growing global demand for protein-rich products and plant promoters.

Cryoprotective Effects and Quality Maintenance of Antifreeze Proteins and Peptides on Aquatic Products: A Review.

Aquatic products are gaining popularity due to their delicacy and high nutrient value. However, they are perishable, with a short shelf-life. Frozen storage is associated with adverse effects, leading to protein oxidation and degradation, thereby altering the protein's structural integrity and subsequently influencing the palatability of protein-based food products. To address these challenges, novel antifreeze peptides have gained significant attention. Antifreeze peptides are a class of small molecular weight proteins or protein hydrolysates that offer protection to organisms in frozen or sub-frozen environments. They offer distinct advantages over conventional commercial antifreeze agents and natural antifreeze proteins. This review provides an overview of the current state of research on antifreeze agents, elucidates their characteristics and mechanisms, and examines their applications in aquatic products. Furthermore, the article offers insights into the prospective development and application prospects of antifreeze peptides.