Higher Nitrogen Use Efficiency Research Articles

Integrated agronomic practice management decreases soil carbon emission and increases environmental ecological benefits of summer maize

Integrated agronomic practices management (IAPM) has been shown as an effective strategy to increase nitrogen use efficiency (NUE) and grain yield during crop production. A field experiment was conducted with four IAPM systems [a local smallholder farmer's practice system (T1), an improved management system (T2), a high–yield production system (T3), and an optimized management system (T4)] to study the annual yield, greenhouse gas (GHG) emissions, carbon footprint (CF), and net environmental ecological benefits (NEEB) of summer maize. The results revealed that the OMS was the optimal choice of IAPM, with increased yield and NUE and reduced GHG emissions and CF. Under OMS, the yield and NUE were 42.6% and 88.1% higher and N 2 O, CO 2 , and CH 4 emissions averaged 35.4%, 5.8%, and 156.5% lower than those of T1. The OMS had the best soil quality, the lowest soil bulk density, the highest C/N value, and the highest total organic carbon among the four treatments, which contributed to reducing GHG emissions. The CF and carbon costs were the lowest under OMS when combined with fertilizer input and GHG emissions. OMS reduced the CF and carbon cost by 5.9% and 33.9%, respectively, and increased the NEEB by 111.4% compared to T1. Although T3 had the highest yield, it had the highest GHG emissions and CF. In conclusion, the OMS achieved a high grain yield and NUE but also mitigate the negative environmental impacts, and increased NEEB, suggesting that it is a suitable management system to increase the productivity and sustainability of summer maize.

Variation of Grain Yield, Grain Protein Content and Nitrogen Use Efficiency Components under Different Nitrogen Rates in Mediterranean Durum Wheat Genotypes

Nitrogen (N) is a crucial nutrient for plant growth and development. To optimize agricultural environments, N fertilizers represent a critical tool to regulate crop productivity. The improvement of nitrogen use efficiency (NUE) represents a promising tool that may enable cereal production to meet future food demand. Wheat reported contrasting behaviors in N utilization showing specific abilities depending on genotype. This study selected two landraces and two improved genotypes from Northern Africa to investigate grain yield (GY), grain protein content (GPC) and NUE. Plants were grown under three levels of N supply: 0, 75, 150 kg N ha−1 and for two consecutive years. Results reported a better NUE (0.40 kg kg N−1) obtained under 150 kg N ha−1, while N utilization efficiency (NUtE) showed a 13% increase using 75 kg N ha−1 compared with 150 kg N ha−1. Under low nitrogen rate (0 N), crop N supply (CNS) and N uptake efficiency (NUpE) were shown as determinant factors for improved genotypes GY (R2 = 0.72), while NUtE represented the most determinant component for GPC in landraces (R2 = 0.92). Multivariate regression models explained the dependence in GPC on NUE, NUpE, and NUtE. In conclusion, our results recognize GPC and NUtE as suitable selection traits to identify durum wheat with higher NUE.

Insights into nitrogen metabolism in the wild and cultivated lettuce as revealed by transcriptome and weighted gene co-expression network analysis

Large amounts of nitrogen fertilizers applied during lettuce (Lactuca sativa L.) production are lost due to leaching or volatilization, causing severe environmental pollution and increased costs of production. Developing lettuce varieties with high nitrogen use efficiency (NUE) is the eco-friendly solution to reduce nitrogen pollution. Hence, in-depth knowledge of nitrogen metabolism and assimilation genes and their regulation is critical for developing high NUE varieties. In this study, we performed comparative transcriptomic analysis of the cultivated lettuce (L. sativa L.) and its wild progenitor (L. serriola) under high and low nitrogen conditions. A total of 2,704 differentially expressed genes were identified. Key enriched biological processes included photosynthesis, oxidation–reduction process, chlorophyll biosynthetic process, and cell redox homeostasis. The transcription factors (TFs) belonging to the ethylene responsive factor family and basic helix-loop-helix family were among the top differentially expressed TFs. Using weighted gene co-expression network analysis we constructed nine co-expression modules. Among these, two modules were further investigated because of their significant association with total nitrogen content and photosynthetic efficiency of photosystem II. Three highly correlated clusters were identified which included hub genes for nitrogen metabolism, secondary metabolites, and carbon assimilation, and were regulated by cluster specific TFs. We found that the expression of nitrogen transportation and assimilation genes varied significantly between the two lettuce species thereby providing the opportunity of introgressing wild alleles into the cultivated germplasm for developing lettuce cultivars with more efficient use of nitrogen.

Heterosis for Nitrogen Use Efficiency of Maize Hybrids Enhanced over Decades in China

The nitrogen use efficiency (NUE) of modern maize hybrids has been greatly improved, but for decades, little was known on whether the NUE heterosis of maize hybrids had increased. A two-year field study was conducted on eight maize hybrids and their parental inbred lines from the 1970s to 2000s under two N levels (0, 150 kg N ha−1). These were the most popular hybrids in China’s main maize-growing areas at the time. The results showed that the yield of the maize hybrids increased significantly at an average rate of 37.5% every 10 years. The evolution of NUE heterosis was mainly related to the increased kernel number per ear. The absolute NUE heterosis (AHNUE) and the mid-parent NUE heterosis (MPHNUE) of maize hybrids increased by 151.4% and 76.4% in the past four decades, or an average rate of 2.11 kg kg−1 and 19.1% every 10 years. Based on the coefficient of determination, the contribution of the mid-parent nitrogen internal efficiency heterosis (MPHNIE) to MPHNUE (43–57%) was significantly higher than that of the mid-parent nitrogen recovery efficiency heterosis (MPHNRE) (19–32%), indicating that the evolution of maize NUE heterosis was mainly derived from its NIE heterosis evolution. The increase of NIE heterosis in the past 40 years was closely related to the increased heterosis of kernel number per ear, the pre-silking N accumulation and the post-silking N remobilization. Therefore, the enhancement of maize NUE heterosis can be attributed to (i) heterosis improvement in post-silking N remobilization, which results mainly from greater heterosis in pre-silking N accumulation; (ii) heterosis improvement in carbon and nitrogen sink capacity, which exhibit as heterosis enhancement in grain yield and grain nitrogen concentration. To further improve yield and NUE, the pre-silking N and carbon accumulation and post-silking N remobilization should both continue to increase in maize breeding. Our results will provide new insights into NUE and help breeders select genotypes with both higher yields and higher NUE for the future.

Effects of Nitrogen Application Rates and Irrigation Regimes on Root Growth and Nitrogen-Use Efficiency of Maize under Alternate Partial Root-Zone Irrigation

Root growth and its adaptation are quite important for efficient use of water and nutrients. The present study investigated the effects of alternate partial root-zone irrigation (APRI) coupled with nitrogen application on root growth and nitrogen-use efficiency (NUE) of maize (Zea mays L.). Treatments (conducted at Wuwei City, northwest China) included APRI with controlled irrigation at 45%, 65%, and 80% of field capacity (FC) (W1, W2, and W3, respectively) coupled with nitrogen application rate of 100, 200, and 300 kg N ha−1 (N1, N2, and N3, respectively). The study found a significant interaction effect of irrigation regimes and nitrogen application rates on maize root growth and NUE. Compared with N2, N3 did not increase the length, surface area, weight, and volume of roots at the filling and maturity stages under W2, while it did under W3, which suggests that increasing nitrogen application rates did not compensate for the adverse effect of drought on root growth. In addition, NUE positively correlated with these root morphological parameters but negatively with the root-to-shoot ratio at the filling and maturity stages. The W2N2 treatment with moderate soil moisture and relatively high soil nitrate-nitrogen promoted total root growth and deeper roots and optimized vertical root distribution, with a low root-to-shoot ratio, resulting in the greatest NUE. These results suggest that moderately controlled irrigation (W2) combined with a reasonable nitrogen rate (N2) improves root growth and optimizes root distribution, resulting in a high NUE in maize under alternate partial root-zone irrigation.

Effect of Combined Application of Slow-Release and Conventional Urea on Yield and Nitrogen Use Efficiency of Rice and Wheat under Full Straw Return

The effects of one-time basal application of different mixtures of slow-release urea (SRU) and conventional urea (CU) on yield and nitrogen use efficiency (NUE) of rice and wheat were investigated to determine the appropriate ratios of SRU to CU for one-time basal fertilization in a rice–wheat rotation farmland under full residue incorporation. A field plot experiment was used in this study. Six treatments were established as follows: CK (no nitrogen fertilizer applied), T0 (100% CU, 50% applied as basal fertilizer and 50% applied as jointing fertilizer), T3 (one-time basal application of SRU and CU mixture with 30% SRU), T5 (one-time basal application of SRU and CU mixture with 50% SRU), T7 (one-time basal application of SRU and CU mixture with 70% SRU), and T10 (one-time basal application of 100% SRU). The results showed that the combined application of SRU and CU increased the yields of rice and wheat. Treatment T7 resulted in the highest rice yield, and T3 resulted in the highest wheat yield, which were 25.6% and 29.4% higher, than those of treatment T0, respectively. Compared with treatment T0 (application of CU alone), the combined application of SRU and CU resulted in 27.4–96.5% and 22.8–57.1% higher NUE in rice and wheat, respectively.

RNA-Seq-Based Transcriptomics Study to Investigate the Genes Governing Nitrogen Use Efficiency in Indian Wheat Cultivars.

High NUE (nitrogen use efficiency) has great practical significance for sustainable crop production. Wheat is one of the main cultivated crops worldwide for human food and nutrition. However, wheat grain productivity is dependent upon cultivars with high NUE in addition to the application of nitrogen fertilizers. In order to understand the molecular mechanisms exhibiting a high NUE response, a comparative transcriptomics study was carried out through RNA-seq analysis to investigate the gene expression that regulates NUE, in root and shoot tissue of N-efficient (PBW677) and N-inefficient (703) cultivars under optimum and nitrogen (N) stress. Differentially expressed gene analysis revealed a total of 2,406 differentially expressed genes (DEGs) present in both the contrasting cultivars under N stress. The efficient genotype PBW677 had considerably more abundant DEGs with 1,653 (903 roots +750 shoots) compared to inefficient cultivar PBW703 with 753 (96 roots +657 shoots). Gene ontology enrichment and pathway analysis of these DEGs suggested that the two cultivars differed in terms of adaptive mechanism. Gene enrichment analysis revealed that among the upregulated and downregulated genes the overrepresented and underrepresented gene categories belonged to biological processes like DNA binding, response to abiotic stimulus, photosynthesis, carbon fixation, carbohydrate metabolic process, nitrogen compound metabolic process, nitrate transport, and translation in cultivar PBW677, while the enriched biological processes were nucleosome assembly, chromatin remodeling, DNA packaging, lipid transport, sulfur compound metabolic process, protein modifications, and protein folding and refolding in N inefficient cultivar PBW703. We found several transcription factors (MYB, WRKY, RING finger protein, zinc finger protein, transporters, NRT1, amino acid transporters, sugar), protein kinases, and genes involved in N absorption, transportation, and assimilation to be highly expressed in high NUE cultivar PBW677. In our study, we report 13 potential candidate genes which showed alternate gene expression in the two contrasting cultivars under study. These genes could serve as potential targets for future breeding programs.

Integrating life cycle assessment and a farmer survey of management practices to study environmental impacts of peach production in Beijing, China.

While intensive peach production has expanded rapidly in recent years, few studies have explored the environmental impacts associated with specific regional systems or the optimal management strategies to minimize associated environmental risks. Here, data from a survey of 290 native farmers were used to conduct a life cycle assessment to quantify the acidification potential (AP), global warming potential (GWP), eutrophication potential (EP), and reactive nitrogen (Nr) losses in peach production in Pinggu District, Beijing. Total annual Nr losses, and GWP, AP, and EP values for peach production in Pinggu District were respectively 10.7kgN t-1, 857kg CO2-eq t-1, 12.9kg SO2-eq t-1, and 4.1kg PO4-eq t-1. The principal driving factors were fertilizer production, transportation, and application, which togetheraccounted for 94%, 67%, 75%, and 94% of Nr losses, GWP, AP, and EP, respectively. In the high yield, high nitrogen-use efficiency (HH) group, relative values of Nr losses, GWP, AP, and EP were respectively 33%, 25%, 39%, and 32% lower than the overall averages for 290 orchards. Further analyses indicate that improved farming practices such as decreasing application rates of fertilizers, increasing proportion of base fertilization rate, and proper fertilization frequency in the HH group were the main reasons for these orchards' better performance in peach yields and partial factor productivity of nitrogen fertilizer, and their reduced environmental impacts. These results highlight the need to optimize nutrient management in peach production in order simultaneously to realize both environmental sustainability and high productivity in the peach production system.

Genetic improvement analysis of nitrogen uptake, utilization, translocation, and distribution in Chinese wheat in Henan Province

Genetic improvement analysis of the nitrogen use efficiency (NUE) of wheat ( Tritium aestivum L.) was undertaken to determine the factors guiding NUE in order to develop new wheat cultivars with high yield, high NUE and high quality. To investigate the genetic improvement of wheat NUE and its components, 31 wheat cultivars that had been widely planted in Henan Province since 1941 were grown under high N treatment (HN) and low N treatment (LN). The results indicated that grain yield and NUE have increased significantly with cultivar development since 1941, with an annual genetic gain of 1.03% and 0.74%, respectively, under HN and LN. The significant increase in NUE mainly resulted from a significant increase in N uptake efficiency (NupE) and N utilization efficiency (NutE). NutE was the predominant component contributing to NUE under HN, while NupE was more important than NutE for NUE under LN. The improvement of NutE occurred at the expense of lower grain N content (GNC) as grain yield increased. Breeding was shown to increase N translocation (NTE) by an annual genetic gain of 1.33% and 0.33%, respectively, under HN and LN. N accumulation at the maturity stage was higher in the modern cultivars compared to the pre-modern cultivars, mainly as a result of increased N accumulation during the stem elongation phase. The N translocation capacity of the vegetative tissues increased, and the N translocation efficiency of the sheath and chaff were higher than that of the leaves. Important genetic improvement targets for high NUE include increasing pre-anthesis N assimilation ability and post-anthesis N translocation capacity. Furthermore improvements in wheat breeding should include increasing both N uptake and NHI to improve NUE and reverse the trend of declining GNC. • Higher genetic gain of grain yield and N use efficiency under better environment. • Modern cultivars had higher N accumulation, mainly raised during stem elongation phase. • N utilization efficiency increased at the expense of lower grain N content. • Enhanced N translocation and uptake was important for N use efficiency improvement.

Lowering nitrogen rates under the system of rice intensification enhanced rice productivity and nitrogen use efficiency in irrigated lowland rice

Among the essential plant nutrients, nitrogen (N) is the most important and universally deficient in rice cropping systems worldwide. Despite different practices available for improvement of N management, nitrogen use efficiency (NUE) is still very low in rice, particularly under conventional management practices. This study was conducted to assess the effect of two crop management practices including the system of rice intensification (SRI) versus conventional management practices (CP) with four N application levels (60, 90, 120, and 150 kg N ha−1) and absolute control (i.e., without N application) on rice growth, grain yield, and NUE. Experiments were established in split-plot randomized complete block design in three replicates. Crop management practices and N levels were treated as the main effect of main-plots and sub-plots, respectively with replicate blocks treated as random factors. Results indicated that deploying of SRI increased rice grain yield by 17.5 and 52.4% during wet and dry seasons, respectively compared with the CP. Rice grain yield was significantly (p < 0.05) higher in SRI than in CP at all levels of N application compared. The application of N at 120 and 60 kg ha−1 resulted in the increase in rice grain yields by 49 and 46.5%, respectively, relative to the absolute control during wet and dry seasons. Nitrogen application had a significant effect (p < 0.05) on agronomic nitrogen use efficiency (ANUE) and partial factor productivity (PFP). Results also indicated that agronomic nitrogen use efficiency (ANUE) was higher (27.2 kg grain kg−1 N) during the wet season with an application of 60 kg N ha−1. Furthermore, higher ANUE (23.8 kg grain kg−1 N) was recorded during dry season with an application of 90 kg N ha−1. The significant (p < 0.05) interaction effects of treatments were recorded on PFP between SRI and 60 kg N ha−1 during the wet (116.7 kg grain kg−1 N) and dry (105.8 kg grain kg−1 N) seasons. This study revealed that ANUE and PFP decreased with N application at the levels of 120 and 150 kg N ha−1 under SRI and CP during the two cropping seasons. The findings of the present study provide potential information that rice grain yield and higher NUE could be achieved at low N inputs under SRI, and thus reducing costs resulted from fertilizer inputs without compromising other environmental benefits.

Effect of nitrogen topdressing on planting density response of grain yield in maize with different planting pattern cultivation

AbstractWe investigated the maize grain yield in response to nitrogen topdressing at different planting densities and planting patterns (twin row, TR; narrow row, NR; and conventional row, CR) over 3 years using early‐maturing cultivars. The P8025 grain yield was higher with a nitrogen topdressing of 6 g/m2 (N6) than without a nitrogen topdressing (N0) at all planting densities in 2017, 2018, and 2019. The difference between the nitrogen topdressing treatments was largest at a planting density close to 10 plants per square meter, which resulted in the highest grain yield in the N0 plot. The rank order for the grain yields among planting patterns was TR ≥ NR &gt; CR in the three analyzed years. Changes in grain yield associated with planting densities followed significant negative quadratic regression curves for all planting patterns. In both the N0 and N6 plots, the grain yield response to planting density was more stable for TR cultivation than for CR and NR cultivation. The rank order for the nitrogen use efficiency (NUE) among planting patterns was TR ≥ NR ≥ CR. The differences in the NUE among planting patterns were higher at higher planting densities. The high NUE observed for TR and NR cultivation was due to the high topdressed nitrogen absorption rate (Nab) at a planting density less than 8.9 plants per square meter as well as the efficiency of the grain yield increase resulting from absorbed nitrogen (Ngy) at a planting density greater than 10.3 plants per square meter. The higher NUE for TR cultivation than for NR cultivation at 12.1 plants per square meter was because of a difference in Ngy and was unrelated to Nab. These results suggest that the improvement of planting pattern (TR, NR) can enhance the effect of topdressed nitrogen on grain yield in maize due to the increase of Nab or Ngy according to the planting density.

Enhancing Sorghum Yield Through Efficient Use of Nitrogen - Challenges and Opportunities.

Sorghum is an important crop, which is widely used as food, forage, fodder and biofuel. Despite its natural adaption to resource-poor and stressful environments, increasing yield potential of sorghum under more favorable conditions holds promise. Nitrogen is the most important nutrient for crops, having a dynamic impact on all growth, yield, and grain-quality-determining processes. Thus, increasing nitrogen use efficiency (NUE) in sorghum would provide opportunities to achieve higher yield and better-quality grain. NUE is a complex trait, which is regulated by several genes. Hence, exploring genetic diversity for NUE can help to develop molecular markers associated with NUE, which can be utilized to develop high NUE sorghum genotypes with greater yield potential. Research on improving NUE in sorghum suggests that, under water-deficit conditions, traits such as stay-green and altered canopy architecture, and under favorable conditions, traits such as an optimized stay-green and senescence ratio and efficient N translocation to grain, are potential breeding targets to develop high NUE sorghum genotypes. Hence, under a wide range of environments, sorghum breeding programs will need to reconsider strategies and develop breeding programs based on environment-specific trait(s) for better adaptation and improvement in productivity and grain quality. Unprecedented progress in sensor-based technology and artificial intelligence in high-throughput phenotyping has provided new horizons to explore complex traits in situ, such as NUE. A better understanding of the genetics and molecular pathways involving NUE, accompanied by targeted high-throughput sensor-based indices, is critical for identifying lines or developing management practices to enhance NUE in sorghum.

OsEIL1 protects rice growth under NH4+ nutrition by regulating OsVTC1-3-dependent N-glycosylation and root NH4+ efflux.

Rice is known for its superior adaptation to ammonium (NH4+ ) as a nitrogen source. Compared to many other cereals, it displays lower NH4+ efflux in roots and higher nitrogen-use efficiency on NH4+ . A critical role for GDP-mannose pyrophosphorylase (VTC1) in controlling root NH4+ fluxes was previously documented in Arabidopsis, but the molecular pathways involved in regulating VTC1-dependent NH4+ efflux remain unclear. Here, we report that ETHYLENE-INSENSITIVE3-LIKE1 (OsEIL1) acts as a key transcription factor regulating OsVTC1-3-dependent NH4+ efflux and protein N-glycosylation in rice grown under NH4+ nutrition. We show that OsEIL1 in rice plays a contrasting role to Arabidopsis-homologous ETHYLENE-INSENSITIVE3 (AtEIN3) and maintains rice growth under NH4+ by stabilizing protein N-glycosylation and reducing root NH4+ efflux. OsEIL1 constrains NH4+ efflux by activation of OsVTC1-3, but not OsVTC1-1 or OsVTC1-8. OsEIL1 binds directly to the promoter EIN3-binding site (EBS) of OsVTC1-3 in vitro and in vivo and acts to increase the transcription of OsVTC1-3. Our work demonstrates an important link between excessive root NH4+ efflux and OsVTC1-3-mediated protein N-glycosylation in rice grown under NH4+ nutrition and identifies OsEIL1 as a direct genetic regulator of OsVTC1-3 expression.

Effect of Azotobacter Chroococcum and Nitrogen Fertilization on Some Morphophysiological Traits, Grain Yield, and Nitrogen Use Efficiency of Safflower Genotypes in Rainfed Conditions

ABSTRACT The effects of Azotobacter chroococcum (A. chroococcum) and nitrogen (N) fertilization on root morphological traits, plant physiology, N uptake of plant, yield components, grain yield, and nitrogen use efficiency (NUE) of six safflower genotypes in rainfed conditions were examined. A factorial experiment was conducted based on the three replications of randomized complete block designs in two cropping years, 2015–2016 and 2016–2017, at Sarableh Agricultural Research Station, Ilam Province, western Iran. Experimental factors included seed inoculation with A. chroococcum using N chemical fertilizer at four levels (two treatments without using N fertilizers urea included control and seed inoculation with A. chroococcum and another two treatments received urea included seed inoculation with A. chroococcum + 50% N of urea source and 100% N of urea chemical source), and six safflower genotypes (312-S6-697, PI-401478, PI-253895, PI-306974, Padideh, and Sina). The results indicated that the highest grain yield, number of grains per boll, and photosynthesis rate were obtained with no significant differences in the treatments of 100% N fertilizer and seed inoculation with A. chroococcum + 50% N fertilizer use. Besides, inoculation with A. chroococcum + 50% N fertilizer had higher NUE than 100% N fertilizer application. Comparing the average genotypes suggested that Sina cultivar with the highest grain yield and NUE was recognized as the best cultivar among the studied genotypes. Therefore, the appropriate cultivars (Sina) with recommended 50% N fertilizer application with A. chroococcum was used to reach the sustainable agriculture under rainfed safflower cultivation. Abbreviations A. chroococcum: Azotobacter chroococcum; BBCH scale: Biologische Bundesantalt, Bun-dessortenamt and Chemische Industrie; N: Nitrogen; NUE: Nitrogen use efficiency

Contribution of the grain size QTL GS3 to yield properties and physiological nitrogen-use efficiency in the large-grain rice cultivar 'Akita 63'.

The development of crop varieties with high nitrogen-use efficiency (NUE) is thought to be important in achieving sustainable cereal crop production. The high yield large-grain rice cultivar Oryza sativa L. ‘Akita 63’ (temperate japonica) has high physiological NUE (PNUE) for grain yield (GY). Our previous study revealed that a large-grain allele of GS3 is present in ‘Akita 63’. Here, we verified the influence of GS3 on the yield properties and PNUE for GY in ‘Akita 63’. The frequency distribution of brown rice length in F2 crosses of ‘Iwate 75’ and ‘Akita 63’ showed a continuous distribution that could be explained by GS3. A near-isogenic line was developed to substitute the GS3 segment of ‘Koshihikari’, which harbours a normal-sized grain allele, in the genetic background of ‘Akita 63’ and the line was designated as Akita63NILGS3-Koshihikari. Compared with Akita63NILGS3-Koshihikari, ‘Akita 63’ exhibited a significantly increased grain length, single brown grain weight and GY, although no significant differences were observed in the nitrogen content and above-ground biomass per unit of cultivated area. These results indicate that the GS3 large-grain allele is a contributing factor to high PNUE for GY in ‘Akita 63’. These findings will facilitate the development of nitrogen-efficient rice varieties.

Dep1 improves rice grain yield and nitrogen use efficiency simultaneously by enhancing nitrogen and dry matter translocation

The rice cultivars carrying dep1 (denseanderectpanicle1) have the potential to achieve both high grain yield and high nitrogen use efficiency (NUE). However, few studies have focused on the agronomic and physiological performance of those cultivars associated with high yield and high NUE under field conditions. Therefore, we evaluated the yield performance and NUE of two near-isogenic lines (NILs) carrying DEP1 (NIL-DEP1) and dep1-1 (NIL-dep1) genes under the Nanjing 6 background at 0 and 120 kg N ha−1. Grain yield and NUE for grain production (NUEg) were 25.5 and 21.9% higher in NIL-dep1 compared to NIL-DEP1 averaged across N treatments and planting years, respectively. The yield advantage of NIL-dep1 over NIL-DEP1 was mainly due to larger sink size (i.e., higher total spikelet number), grain-filling percentage, total dry matter production, and harvest index. N utilization rather than N uptake contributed to the high yield of NIL-dep1. Significantly higher NUEg in NIL-dep1 was associated with higher N and dry matter translocation efficiency, lower leaf and stem N concentration at maturity, and higher glutamine synthetase (GS) activity in leaves. In conclusion, dep1 improved grain yield and NUE by increasing N and dry matter transport due to higher leaf GS activity under field conditions during the grain-filling period.

Introgression of qPE9-1/DEP1, a major QTL for rice panicle erectness, drastically improves nitrogen use efficiency under limited nitrogen supply

The majority of japonica rice cultivars currently grown in China are of the erect panicle (EP) type, which shows greater yield potential than the drooping panicle type. However, EP cultivars require higher nitrogen (N) input to maintain an acceptably high grain yield. Although a crucial quantitative trait locus (QTL), qPE9–1/DEP1, for the EP type has been identified, its genetic effects on grain yield remain controversial. ‘Wuyunjing 8′ (WYJ8) is a typical EP cultivar harboring the naturally mutated qpe9–1 allele. WYJ8_qPE9–1 is a transgenic line carrying the qPE9–1 allele in the WYJ8 background. To explore the genetic effects of the qPE9–1/DEP1 alleles, we conducted field experiments to compare the responses of WYJ8 and WYJ8_qPE9–1 to different N supply rates and planting densities in three consecutive years. WYJ8_qPE9–1 outperformed WYJ8 in grain yield and nitrogen use efficiency (NUE) under low N supply and low planting density, whereas WYJ8 excelled under excessive N supply and high planting density. The differential response in yield of the two genotypes to N supply was predominantly attributable to changes in panicle number and spikelet number. NUE-associated genes responded to N supply rates differently in the WYJ8_qPE9–1 and WYJ8, suggesting that a coordinated, complex network of genes is involved in NUE and subject to regulation by qPE9–1/qpe9–1. The results demonstrated that cultivars carrying qPE9–1 or qpe9–1 require different management practices to achieve yield potentials and high NUE. Adoption of qPE9–1 can enhance grain yield and NUE under low to moderate N supply and low planting density, whereas qpe9–1 can be utilized to exploit excessive N supply and high planting density.

Coupling effects of irrigation amount and nitrogen fertilizer type on grain yield, water productivity and nitrogen use efficiency of drip-irrigated maize

Agricultural production is facing the risk of severe water shortage, serious non-point source pollution and low water productivity (WP) and nitrogen use efficiency (NUE). It is a major challenge to achieve environmentally friendly production of maize with high WP and NUE. A two-year field experiment with three irrigation levels of W1 (60% ETc, ETc is the crop evapotranspiration), W2 (80% ETc) and W3 (100% ETc), and three nitrogen (N) fertilizer types of urea (U), slow-release N fertilizer (SRF) and urea blended with slow-release N fertilizer (UNS) was conducted in 2019 and 2020, so as to quantify their impacts on the maize dry matter, N uptake and remobilization, grain yield, WP, NUE and residual soil NO3−-N. The results showed that W2 and W3 significantly increased total dry matter (8.0% and 14.7%), grain yield (19.4% and 33.0%), and NUE (1.5% and 2.2%) compared with W1. Total N uptake of maize declined by 23.2% and 9.6% under W1 and W2, leading to 11.9% and 5.8% increased of soil NO3−-N, respectively. Compared with U, UNS increased post-silking N uptake by 19.1%, thereby enhancing grain yield by 18.3%. Compared with SRF, UNS increased N remobilization from vegetative organs to grains by 15.0%, thereby resulting in 2.3% increase in grain yield. Under the same irrigation amount, UNS and SRF improved WP and NUE compared with U. W3SRF obtained the highest grain yield, with increases in grain yield by 2.4–58.8% compared with other treatments, while there was no significant difference between W3SRF and W3UNS. More importantly, UNS significantly increased grain yield and alleviated the impact of water stress on maize production under W1 and W2. In conclusion, UNS could stabilize grain yield under water-stressed conditions, while SRF could increase grain yield under full irrigation.

Identification of High Nitrogen Use Efficiency Phenotype in Rice (Oryza sativa L.) Through Entire Growth Duration by Unmanned Aerial Vehicle Multispectral Imagery.

Identification of high Nitrogen Use Efficiency (NUE) phenotypes has been a long-standing challenge in breeding rice and sustainable agriculture to reduce the costs of nitrogen (N) fertilizers. There are two main challenges: (1) high NUE genetic sources are biologically scarce and (2) on the technical side, few easy, non-destructive, and reliable methodologies are available to evaluate plant N variations through the entire growth duration (GD). To overcome the challenges, we captured a unique higher NUE phenotype in rice as a dynamic time-series N variation curve through the entire GD analysis by canopy reflectance data collected by Unmanned Aerial Vehicle Remote Sensing Platform (UAV-RSP) for the first time. LY9348 was a high NUE rice variety with high Nitrogen Uptake Efficiency (NUpE) and high Nitrogen Utilization Efficiency (NUtE) shown in nitrogen dosage field analysis. Its canopy nitrogen content (CNC) was analyzed by the high-throughput UAV-RSP to screen two mixed categories (51 versus 42 varieties) selected from representative higher NUE indica rice collections. Five Vegetation Indices (VIs) were compared, and the Normalized Difference Red Edge Index (NDRE) showed the highest correlation with CNC (r = 0.80). Six key developmental stages of rice varieties were compared from transplantation to maturation, and the high NUE phenotype of LY9348 was shown as a dynamic N accumulation curve, where it was moderately high during the vegetative developmental stages but considerably higher in the reproductive developmental stages with a slower reduction rate. CNC curves of different rice varieties were analyzed to construct two non-linear regression models between N% or N% × leaf area index (LAI) with NDRE separately. Both models could determine the specific phenotype with the coefficient of determination (R2) above 0.61 (Model I) and 0.86 (Model II). Parameters influencing the correlation accuracy between NDRE and N% were found to be better by removing the tillering stage data, separating the short and long GD varieties for the analysis and adding canopy structures, such as LAI, into consideration. The high NUE phenotype of LY9348 could be traced and reidentified across different years, locations, and genetic germplasm groups. Therefore, an effective and reliable high-throughput method was proposed for assisting the selection of the high NUE breeding phenotype.

338 Nitrogen Use Efficiency Is Proportionally More Improved When Young Bulls Are Ranked on Residual Body Gain Rather Than on Residual Feed Intake

Abstract Animals with superior feed efficiency (FE) may also have an improved nitrogen use efficiency (NUE), which would be beneficial to economic profitability while reducing environmental impacts. When genetically selecting animals on FE, it is preferable to use residual traits [e.g. residual feed intake (RFI) or residual body gain (RG)] rather than ratios because of their predictable genetic outcomes. We studied the relationship of RFI and RG with NUE, estimated from the validated 15N abundance in plasma, across two contrasted diets based on corn or grass silages. We evaluated FE of 588 (half by diet) Charolais bulls (545 ± 57 kg BW) from 12 experimental cohorts (different farms and periods) over 200 days. Before the end of the FE test, plasma was sampled and analyzed for δ 15N. NUE was related to FE through simple-linear models with variables previously corrected for the cohort and diet effects. The models’ slopes were standardized according to FE deviation in order to compare the response of NUE to FE between indices. Higher NUE was related to higher FE (P &amp;lt; 0.001), showing positive correlations with RG (r=-0.40) and negative with RFI (r=0.29). However, the standardized slope of NUE to RG was significantly higher (+28%; P &amp;lt; 0.05) than that of NUE to RFI. This stronger NUE relation to RG compared to RFI could reflect a higher potential of RG animals to deposit N as compared to a more conservational N metabolism in RFI individuals. Regarding diets, and despite the correction of NUE and FE for this effect, the slopes of NUE to FE were numerically (P &amp;gt; 0.05) higher (-16% and +36%; for RG and RFI) in corn-based diets, which agrees with superior NUE observed in corn-vs-grass diets. Results suggested that superior RG animals may present proportionally higher NUE than superior RFI animals, with even better results in corn-vs-grass diets.