High Mountain Ecosystems Research Articles

Contrasting habitat associations and ecophysiological adaptations drive interspecific growth differences among Himalayan high-mountain plants.

Understanding interspecific differences in plant growth rates and their internal and external drivers is key to predicting species responses to ongoing environmental changes. Annual growth rates vary among plants based on their ecological preferences, growth forms, ecophysiological adaptations, and evolutionary history. However, the relative importance of these factors remains unclear, particularly in high-mountain ecosystems experiencing rapid changes. We examined how habitat associations, elevational optima, growth forms, and ecophysiological and anatomical traits influence interspecific differences in radial growth rates among 324 vascular dicot species naturally occurring in the western Himalayas. Growth rates were determined from annual ring width measurements on the oldest plant sections of over 7,800 individuals from a range of habitats (desert, steppe, wetland, alpine, subnival), growth forms (perennial tap-rooted, rhizomatous, cushiony, woody), and climatic gradients (elevations of 2,650-6,150 m). Habitat associations accounted for 24% of the variability in interspecific growth rates. Adding growth form and height increased the explanation to 42%, and incorporating plant functional traits further improved predictions to 46%. Growth rates were higher in warmer, drier conditions and lower in cold, wet environments. Subnival cushion plants had the slowest growth, while ruderal plants grew the fastest. Desert plants showed higher growth rates, reflecting their drought adaptive strategies, while wetland forbs had lower growth rates due to increased resource competition. Growth was positively correlated with leaf nitrogen content and non-structural carbohydrates (mainly fructans), due to enhanced photosynthesis and stress tolerance, and negatively correlated with leaf carbon and root nitrogen content. Our study of 324 dicot species in the western Himalayas suggests that plant growth in high elevations is determined by a combination of habitat conditions, morphological traits, and ecophysiological adaptations. Growth variations among the highest-growing angiosperms reflect adaptive strategies along the global 'fast-slow' and 'acquisitive-conservative' spectrums. These results underscore the importance of habitat-specific studies for predicting plant growth responses to environmental changes, emphasizing a species-specific approach for effective conservation in fragile ecosystems.

Integration of InSAR coherence and SAR backscatter increases accuracy of LULC mapping in tropical high-mountain ecosystems

Páramos, neotropical high-mountain biomes of the northern Andes, are increasingly threatened by human land use and climate change. Accurate land use/land cover (LULC) mapping is critical for monitoring these pressures. However, persistent cloud cover poses substantial challenges for optical satellite imagery. This study leverages multitemporal Interferometry SAR (InSAR) coherence and dual polarimetric backscatter intensity to improve LULC mapping accuracy in páramos ecosystems. Using a 1-year Sentinel-1 time series from January to December 2019, 17 LULC classes were mapped across 17,000 hectares. Results demonstrate that integrating backscatter and coherence increased F1-Score and overall accuracy by 9% and 11%, respectively, compared to backscatter alone. This emphasizes the critical role of multitemporal SAR for LULC mapping in cloud-prone regions. Additionally, this study underscores the utility of cloud-based platforms for efficient SAR data processing, offering a scalable approach for mapping high-mountain ecosystems worldwide, especially in inaccessible and heavy precipitation regions.

Seronegativity of Iberian ibex (Capra pyrenaica) against Toxoplasma gondii and Neospora caninum is consistent with eco-epidemiological and environmental features in Mediterranean mountainous areas.

Knowledge of pathogen epidemiological dynamics and habitat ecological features is essential for wildlife population and health monitoring and management. Toxoplasma gondii and Neospora caninum are two broadly distributed multi-host parasites that affect both wild and domestic animals and, in the case of T. gondii, cause zoonosis. This study reports the seroprevalence of both parasites in Iberian ibex (Capra pyrenaica), a mountain wild ruminant native to the Iberian Peninsula, from the Natural Space of Sierra Nevada (NSSN) in southeastern Spain. Serum from 146 Iberian ibexes were analysed using two in-house ELISA techniques. The positive and doubtful sera were further checked by Western Blot (WB). Seventeen ibexes (11.6%; 95% confidence interval 6.4-16.7) were positive for T. gondii and seven (4.8%; 95% confidence interval 1.3-8.2) for N. caninum. However, no sera were positive to T. gondii nor to N. caninum by WB. Using at least two different serological techniques is recommended when they are not validated for the target host species. The NSSN is a hypoendemic area for T. gondii and N. caninum, probably determined by the reduced abundance and restricted distribution of their definitive hosts. This would explain the hypoendemic situation in the NSSN and the lack of specific antibodies against these two parasites in the Iberian ibex population. This eco-epidemiological scenario can be challenged by climate and anthropogenic changes, recommending long-term monitoring Iberian ibex population and health, both as a conservation measure for the species and as an indicator of the potential impact of global change on high mountain ecosystems.

Forecasting Range Shifts in Terrestrial Alpine Insects Under Global Warming.

Anthropogenic planetary heating is disrupting global alpine systems, but our ability to empirically measure and predict responses in alpine species distributions is impaired by a lack of comprehensive data and technical limitations. We conducted a comprehensive, semi-quantitative review of empirical studies on contemporary range shifts in alpine insects driven by climate heating, drawing attention to methodological issues and potential biotic and abiotic factors influencing variation in responses. We highlight case studies showing how range dynamics may affect standing genetic variation and adaptive potential, and discuss how data integration frameworks can improve forecasts. Although biotic and abiotic factors influence individual species responses, most alpine insects studied so far are shifting to higher elevations. Upslope shifts are often accompanied by range contractions that are expected to diminish species genetic variation and adaptive potential, increasing extinction risk. Endemic species on islands are predicted to be especially vulnerable. Inferences drawn from the responses of alpine insects, also have relevance to species in other montane habitats. Correlative niche modelling is a keystone tool to predict range responses to planetary heating, but its limited ability to consider biological processes underpinning species' responses complicates interpretation. Alpine insects exhibit some potential to respond to rising temperatures via genetic change or phenotypic plasticity. Thus, future efforts should incorporate biological processes by using flexible hybrid niche modelling approaches to enhance the biological realism of predictions. Boosting scientific capability to envisage the future of alpine environments and their associated biota is imperative given that the speed and intensity of heating on high-mountain ecosystems can surpass our ability to collect the empirical data required to guide effective conservation planning and management decisions.

Influence of water chemistry and contaminant occurrence on the oxidative stress ecology of Cottus gobio in a high-mountain lake (Carnic Alps)

Understanding oxidative stress in high-mountain lake fish offers crucial insights into their health, resilience, and adaptation to extreme environmental changes. This study investigates the oxidative stress response of Cottus gobio in a high-mountain lake (Dimon Lake) located in the northeast Italy during the ice-free season, focusing on the relationship between oxidative stress biomarkers and physicochemical water parameters, as well as persistent and emerging contaminants. Significant seasonal variations were observed in water parameters, with lower oxygen, pH, conductivity, and phosphate levels in summer compared to autumn, while temperature, ammonium, and nitrate were higher in summer. Metal concentrations in C. gobio muscle were higher in autumn, with Zn showing the most significant increase. PAHs, NDL-PCBs, and pesticides were all below the limit of quantification in the fish muscle samples. No microplastics items were found in the gastrointestinal tracts of fish. Oxidative stress biomarkers revealed organ-specific and seasonal variations. The liver exhibited the highest activities of catalase (CAT), glutathione peroxidase (GPx), glutathione S-tranferase (GST), and glutathione reductase (GR), highlighting its central role in detoxification and metabolic processes. Superoxide dismutase (SOD) activity was notably higher in muscle tissue during summer, suggesting increased metabolic activity. A strong correlation was found between pH and the activities of SOD, CAT, GPx, GR, and metallothioneins (MTs), emphasizing the importance of water chemistry in influencing oxidative stress responses. This approach not only aids in understanding the local adaptations of these fish but also highlights the impacts of environmental stressors on high-mountain ecosystems. Continuous monitoring of water chemistry, particularly pH, is crucial for understanding and managing oxidative stress in aquatic organisms, especially in the context of global environmental changes.

Ecological analysis of the structure and dendrometric characteristics of the population of Juniperus thurifera: a vulnerable species

ABSTRACT Juniperus thurifera was selected for this study due to its crucial ecological role in high mountain ecosystems and the increasing threats it faces, such as habitat degradation and regeneration challenges. The analysis of stand structure by altitude shows that the distribution of individuals and stem count vary with elevation, highlighting the species’ adaptability to mountainous conditions. Altitudinal degradation significantly impacts seed production, with a positive correlation (r = 0.65) between stem and fruit numbers in females. J. thurifera also plays a vital role in soil retention, as indicated by the positive correlation (r = 0.511) between ground projection and soil thickness, emphasizing its importance in slope stabilization and erosion prevention. Sexual dimorphism reveals dendrometric differences between males and females, affecting regeneration and reproduction. Age estimation based on circumference shows the predominance of aging, non-reproductive trees, threatening future regeneration. Principal component analysis (PCA) further refines recommendations by revealing complex relationships between dendrometric characteristics and environmental factors, providing precise guidelines for J. thurifera conservation.

Vulnerability of Nature Based Tourism to Climate Change in Risaralda, Colombia

The study presents an approach to assess the vulnerability of nature-based tourism in Risaralda, Colombia, to climate change. An environmental reference model is adapted to the tourism sector. The results reveal high vulnerability at the municipal level, conservation areas, ecosystems and tourism supply, especially in high mountain ecosystems. This underlines the importance of adaptive tourism management and measures to reduce vulnerability, such as avoiding concentration of tourism supply, since some of the most vulnerable tourism destinations are paradoxically the most competitive in terms of sustainability. This approach is applicable to other tourism destinations.

Monitoring of snow leopards in the Sarychat-Ertash State Reserve (Kyrgyzstan), between 2011 and 2019, through scat genotyping

Snow leopards (Panthera uncia) are a keystone species of Asia’s high mountain ecosystem. The species is assessed as Vulnerable by the IUCN Red List of Threatened Species and is elusive, limiting accurate population assessments that could inform conservation actions. Non-invasive genetic monitoring conducted by citizen scientists offers avenues to provide key data on this species. From 2011 to 2019, OSI-Panthera citizen science expeditions tracked signs of presence of snow leopards and collected scat samples along transects in the main valleys and crests of the Sarychat-Ertash State Reserve (Kyrgyzstan). Scat samples were genotyped at twenty autosomal microsatellite loci and at a X/Y locus (sex identification), allowing an estimation of a minimum of 17 individuals. The genetic recapture of 12 of them provided indications of individuals’ habitat use throughout the reserve. We found putative family relationships between several individuals; however, further research is needed to validate these findings. Our results demonstrate the potential of a citizen science program to collect meaningful data that can inform the conservation management of snow leopards.

Microplastic assessment in remote and high mountain lakes of Gilgit Baltistan, Pakistan

Microplastic (MP) pollution is a critical environmental challenge worldwide, however limited research is reported in remote lakes of Pakistan. This study assessed MPs (>5 mm) prevalence, distribution and risk perspective in water and sediment of eight remote and high-altitude lakes (>1500 m above sea level) of Gilgit Baltistan, Pakistan. The lakes exhibited an average abundance of 152.6 ± 104.6 to 12.1 ± 7 MP/kg of dry sediments and 2 ± 0.9 to 17.1 ± 17.2 MP/L of surface water. MPs <200 μm dominated in both matrices. Surface water predominantly contained polyester and polypropylene, while polypropylene and polyethylene dominated in sediments. The gradient of elevation did not show any pronounced impact on the fiber loading or MP count in both matrices. Backward air mass trajectory revealed that air masses vastly travelled from western-Asia, Arabian sea and Bay of Bengal with an average transmission distance of 2500–3500 km (500 m a.s.l) that can be a potential deposition MP source in the area. Pollution Load Index of the lakes were >1 exhibiting pollution. All other lakes except Batura and Borith manifested a moderate hazard index. Naltar lake along with aforementioned two lakes also manifested high polymer toxicity. Further research should emphasize understanding the mechanisms and biotic interactions in high-mountain ecosystems.

Demography and natural history of the damselfly Mesamphiagrion gaudiimontanum (Coenagrionidae), a Páramo endemic species in the Colombian Andes

The páramos are high mountain ecosystems in the tropical regions of the New World, and they are particularly threatened by climate change. The Belmira páramo complex in Colombia is home to Mesamphiagrion gaudiimontanum, an endemic and endangered damselfly species. This study presents the first demographic assessment of M. gaudii­montanum using the capture-recapture method to estimate population size, survival, and detection probabilities, focusing on differences in age, sex, female color morphs, and parasite infestation. The population size was estimated to be 730 ± 186.59 individuals, with an average survival probability of 0.89 ± 0.04 and a life expectancy of 8.34 days, these data are like the reported for other populations in the same genus in harsh environmental conditions. Ectoparasitic mites were more common on juveniles than adults. After several hours of observing the species few copula events have been recorded, all of them involving juvenile adult females, which points to a monandrous reproduction syndrome for the species. Another phenomenon observed in the species was a change in coloration due to body temperature in individuals, in which bright coloration becomes grayish in cold weather. This study provides fundamental data for future monitoring and conservation efforts, highlighting the species’ vulnerability to environmental changes and the need for specific conservation strategies.

Mosaic coexistence of two subalpine grassland types as a consequence of soil nutrient heterogeneity

High-mountain areas often exhibit high soil heterogeneity, which allows for the close coexistence of plant species and communities with contrasting resource requirements. This study investigated the nutritional factors driving the mosaic distribution of Nardus stricta L. grasslands and chalk grasslands dominated by forbs in the subalpine southern Pyrenees (Spain). The concentrations of C, N, P, S, K, Ca and fiber fractions in herbage were analyzed in relation to soil nutrient availability; soil β-glucosidase, urease, phosphatase and arylsulfatase activities; and plant species and functional type compositions. The chalk grassland showed higher N:P ratios in herbage and higher enzyme demand for P relative to N in the soil, which indicates a greater limitation of P versus N compared to Nardus grassland. This limitation was related to the higher soil and plant Ca levels in the chalk grassland, where the calcareous bedrock lies close to the soil surface. In the Nardus grasslands, the alleviation of P limitation translated into increased productivity and the replacement of forbs with taller graminoids rich in structural carbohydrates, which was mirrored by greater β-D-glucosidase activity. The plant N:K and P:K ratios indicated potential K deficiency in both grasslands, which resulted from decreased uptake of K in competition with Ca, as indicated by the correlation between plant K and the soil K+:Ca2+ ratio. Our results highlight the effect of the heterogeneity of soil nutrient constraints, mediated by stoichiometry and dependent on microtopography, on the biodiversity of high-mountain ecosystems.

Increasing growth sensitivity of Pinus wallichiana to summer season maximum temperature – evidence from central Bhutan Himalaya

The impacts of global warming are pervasive across different forest biomes and are most pro­nounced in high mountain ecosystems such as the Himalayas. This study examines the growth response of Blue pine (Pinus wallichiana) to climate from Bumthang district in the central Bhutan Himalaya. Tree radial growth parameters (ring width index and basal area increment) were correlated with monthly and daily climate. Temporal changes in significant climate response were assessed using running correlations. Irrespective of tree growth parameters or temporal resolution of climatic variables, growth-climate re­sponse revealed maximum temperature during summer as the most significant climatic factor regulating the growth of P. wallichiana in central Bhutan. The strength and stability of the climate response improved when using daily climate, which is not restricted by non-biological calendar months. Around the turn of the 21st century, we observed a rapid rise in radial growth. Over the last ~30 years, blue pine from central Bhutan has benefited from warming summer season maximum temperatures. This study highlights the importance of considering daily climate variables for more accurate assessments of tree growth responses to climate. These findings have significant implications for understanding the impact of climate change on forests in the Himalayan region.

Socio-ecological system of the mountainous region: A case study from Georgia

Aim: The research aim to study the change to characteristics of the socio-ecological system of the mountain regions of Georgia. Methodology: Research methods included geographic, cartographic, ecosystem, and landscape research methodology, landscape-ecological analysis and synthesis methods, and social-ecological systems concepts. Results: The results showed the following: Ecological characteristics of medium and high mountain natural landscapes and ecosystems within the Central Caucasus (Georgia's historical-geographical province of Pshav-Khevsureti); Main forms of cultural landscape, their economic and social values of the mountain region; Environmental protection of the local population, ethics and environmental history and the ecological, economic, and social culture of the population of the mountain region. Interpretation: In the mountainous regions of Georgia, there is a high potential for creating cultural landscapes, based on the results of the long-term and effective interdependence of man and nature. Cultural landscapes, depending on their importance, will further increase the sustainability of the socio-ecological system of mountainous regions. Key words: Cultural landscape, Ecological culture, Georgia, Mountainous regions, Socio-ecological syste

Land cover changes and comparison of current landscape metrics in a region of the Central Andes affected by population migration

ABSTRACT The Andes region has a rich history of environmental and human interactions that has shaped the landscape for millennia. Our study quantified the land cover changes in the districts of Huanta and Luricocha after the human population abandoned the region due to the armed conflict with Peruvian authorities (1980 – 1990) and their progressive return (1990 – 2020). We analysed satellite images of various resolutions, including Landsat-5 for the years 1986, 1990, 2000 and 2010; Landsat-8 for the year 2020, Sentinel-2 for the years 2017 and 2020; PeruSat-1 for the years 2017 and 2020, to assess the temporal change of land cover and landscape metrics. The classification was based on the pixel approach for Landsat images and Sentinel-2 with an average accuracy of 88.29% and 92.13% respectively, and an object-based (GEOBIA) approach for Perusat-1 images, with an average accuracy of 87.75%. The dominant coverages are high Andean grasslands, scrublands and croplands. According to the obtained data, high Andean grasslands and scrublands have decreased, which is associated with an increase in population over time. Likewise, there has been an increase in the area of lagoons and the expansion of nearby wetlands due to the construction of dams, driven by an increase in demand for water for human consumption and agriculture. However, areas with degraded soils, rocky outcrops, and forest plantations have expanded. The results suggest that abandoned agricultural lands did not recover quickly enough and more research and action is needed for restoration of the high-mountain ecosystem. Landscape metrics such as Edge Density (ED), Euclidean Distance to Nearest Neighbor (ENN) and Aggregation Index (AI) were calculated for different land cover (LC/LU) to characterize composition and structure, showing their variation at different spatial resolutions. This indicates that increasing spatial resolution makes it possible to better define the landscape characteristics.

High mountain agriculture in Colombia: opportunities for conversion to sustainability

This review article presents a socioecological analysis based on current literature to provide elements to enrich the currentdebate on the transition to sustainability proposed in Law 1930 of 2018. to achieve this objective, a review of currentregulations and commitments of Colombia for sustainable agriculture is made, Afterwards, a review of high mountainecosystems and their importance with emphasis on agricultural and livestock activities is included. Subsequently, adescription of the different schools of agriculture present in high mountain ecosystems is included. Finally, a reflection ismade regarding the progress and challenges to achieve the transition of agricultural activities towards sustainability inmoorland ecosystems. It is concluded that, although there are advances in the political-institutional and socio-ecologicalframework, it is imperative to focus on all the actors of the Colombian agri-food system and make timely progress in therecognition and valuation of the contributions that the different schools of agriculture make to sustainability, usingcriteria that are proposed based on the dimensions of sustainability. Not including these elements in the debate turns thetransition into a sophism that will perpetuate socio-ecological conflicts in the territories.

Assessing the occurrence of soil improvement and its relationship to the dominant life form in the high mountains of Central Spain

Soil in mountainous regions is vital to the health and preservation of these unique and diverse ecosystems. In dry and semi-arid regions, vegetation patches play a crucial role in soil nutrient heterogeneity through continuous feedback with soils, acting as barriers to collect runoff water, sediments, and nutrients from bare soil regions. Soil amelioration, an enhancement of soil biogeochemical processes, results in the formation of “fertility islands,” the extent of which is contingent on the plant species in question, as well as nutrient dynamics and water availability. Here, we collected soil from across a two-peak altitudinal gradient in the Sierra de Guadarrama high-mountains, each peak featuring a different dominant vegetation type (herbs vs. cushion-like) to compare soil nutrients and properties between bare soil and vegetation-covered patches (microhabitats). Soil improvement was assessed in the microhabitats using the Relative Interaction Index (RII). Fertility islands were shown to be prevalent in high-mountain ecosystems, as soil quality and properties were higher beneath vegetation-covered regions than bare soils. There was a difference in RIIs between the transects, with greater soil improvement in the cushion-dominated transect than the herb-dominated one. Changes in nutrient levels were unrelated to patch successional stage, indicaing that plant generations may not shape the spatial variability of soil attributes. Instead, species variety or the presence of dominant clonal species increased soil nutrients and aggregate stability, highlighting the importance of root shape and high biomass in nutrient retention and soil reinforcement. Finally, our findings imply that the poor, shallow soils in the examined peaks, in comparison to other mountains, may account for the poor facilitative interactions. Competition for the scarce resources at these peaks may intensify as climate warms. Thus, while these plants may grow with minimum assistance under current climate circumstances, their associations may be especially vulnerable to climate change.

ASSESSING THE SATISFACTION OF DOMESTIC TOURISTS WITH ECO-TOURISM DEVELOPMENT IN MANG DEN TOWN, KON PLONG DISTRICT, KON TUM PROVINCE, VIETNAM

Mang Den town, Kon Plong District, the Central Province of Kon Tum is one of the destinations of recent interest. With the advantage of the climate and unique high mountain ecosystem, eco-tourism development is one of the notable trends in Mang Den. Based on a survey of 130 domestic tourists to Mang Den, the study used SPSS software 20 to analyze domestic tourists' satisfaction with eco-tourism development in Mang Den. The findings of this research demonstrated the potential of ecotourism Mang Den Town as a destination for Vietnamese tourists seeking natural beauty, cultural experiences, and adventure activities. The positive satisfaction levels indicated that efforts have been made to provide a quality tourism experience. However, there is still room for improvement, particularly in infrastructure development and providing more interpretive and accommodation options. These results can guide tourism stakeholders in developing strategies to enhance ecotourism in Mang Den Town, as a Good Practice and ensure sustainable tourism growth in the Kon Plong District, Kon Tum Province.&lt;p&gt; &lt;/p&gt;&lt;p&gt;&lt;strong&gt; Article visualizations:&lt;/strong&gt;&lt;/p&gt;&lt;p&gt;&lt;img src="/-counters-/soc/0089/a.php" alt="Hit counter" /&gt;&lt;/p&gt;

Glacier loss and vegetation expansion alter organic and inorganic carbon dynamics in high-mountain streams

Abstract. High-mountain ecosystems are experiencing the acute effects of climate change, most visibly through glacier recession and the greening of the terrestrial environment. The streams draining these landscapes are affected by these shifts, integrating hydrologic, geologic, and biological signals across the catchment. We examined the organic and inorganic carbon dynamics of streams in four Alpine catchments in Switzerland to assess how glacier loss and vegetation expansion are affecting the carbon cycle of these high-mountain ecosystems. We find that the organic carbon concentration and fluorescence properties associated with humic-like compounds increase with vegetation cover within a catchment, demonstrating the increasing importance of allochthonous dissolved organic carbon sources following glacier retreat. Meanwhile, streams transitioned from carbon dioxide sinks to sources with decreasing glacier coverage and increased vegetation coverage, with chemical weathering and soil respiration likely determining the balance. Periods of sink behavior were also observed in non-glaciated streams, possibly indicating that the chemical consumption of carbon dioxide could be more common in high-mountain, minimally vegetated catchments than previously known. Together, these results demonstrate the dramatic shifts in carbon dynamics of high-mountain streams following glacier recession, with significant changes to both the organic and inorganic carbon cycles. The clear link between the terrestrial and aquatic zones further emphasizes the coupled dynamics with which all hydrologic and biogeochemical changes in these ecosystems should be considered, including the carbon sink or source potential of montane ecosystems.

Systematics, morphometry, and distribution of Eptesicus fuscus miradorensis (H. Allen, 1866) (Chiroptera: Vespertilionidae), with notes on baculum morphology and natural history

The brown bat Eptesicus fuscus (Chiroptera: Vespertilionidae) is a widely distributed species with up to 11 subspecies ranging from North America, some Caribbean islands, and Central and northern South America. Within the species, Eptesicus fuscus miradorensis occurs from North America to South America being the only subspecies that occurs in the continental area of the Neotropical region and might be considered a full species. Also, it has been suggested that E. f. miradorensis shows a clinal morphologic variation from the northernmost populations of Central America toward South America. We evaluated the systematic position of E. f. miradorensis using genetic samples from Central and South America. In addition, we assessed the morphometric variations of E. f. miradorensis using 14 external and cranial measurements of specimens distributed along America. To evaluate the clinal variation and interspecific changes through its distribution, we assigned three groups considering the localities of origin i) North (México), ii) Center (Guatemala-Panamá), and iii) South (Colombia-Venezuela) using multivariate analyzes. We also compiled the localities of the revised specimens and these from databases to determine the environmental factors that potentially constrain the distribution of the taxon. We suggest that E. f. miradorensis should be elevated to the species level based on genetic comparisons. Additionally, we did not find sexual dimorphism or size variation associated with its distribution. The species is distributed from México to South America (Colombia, Venezuela, and Ecuador) in elevations that average over 1,000 m, showing a strong association with high mountain ecosystems. This taxon increases to 12 the number of species of bats of the subgenus Eptesicus in South America.

High Diversity of Bradyrhizobial Species Fix Nitrogen with Woody Legume Spartocytisus supranubius in a High Mountain Ecosystem.

The symbiosis between rhizobia and legumes is of pivotal importance in nitrogen-poor ecosystems. Furthermore, as it is a specific process (most legumes only establish a symbiosis with certain rhizobia), it is of great interest to know which rhizobia are able to nodulate key legumes in a specific habitat. This study describes the diversity of the rhizobia that are able to nodulate the shrub legume Spartocytisus supranubius in the harsh environmental conditions of the high mountain ecosystem of Teide National Park (Tenerife). The diversity of microsymbionts nodulating S. supranubius was estimated from a phylogenetic analysis of root nodule bacteria isolated from soils at three selected locations in the park. The results showed that a high diversity of species of Bradyrhizobium and two symbiovars can nodulate this legume. Phylogenies of ribosomal and housekeeping genes showed these strains distributed into three main clusters and a few isolates on separate branches. These clusters consist of strains representing three new phylogenetic lineages of the genus Bradyrhizobium. Two of these lineages belong to the B. japonicum superclade, which we refer to as B. canariense-like and B. hipponense-like, as the type strains of these species are the closest species to our isolates. The third main group was clustered within the B. elkanii superclade and is referred to as B. algeriense-like as B. algeriense is its closest species. This is the first time that bradyrhizobia of the B. elkanii superclade have been reported for the canarian genista. Furthermore, our results suggest that these three main groups might belong to potential new species of the genus Bradyrhizobium. Analysis of the soil physicochemical properties of the three study sites showed some significant differences in several parameters, which, however, did not have a major influence on the distribution of bradyrhizobial genotypes at the different locations. The B. algeriense-like group had a more restrictive distribution pattern, while the other two lineages were detected in all of the soils. This suggests that the microsymbionts are well adapted to the harsh environmental conditions of Teide National Park.