AbstractFour pot experiments were performed with five calcareous soils from western Minnesota (Haplaquolls and Calciaquolls) to determine the effects of soil moisture and soil temperature on Fe chlorosis in soybeans [Glycine max (L.) Merr.]. We used a factorial design with gravimetric moisture contents and bulk density values ranging from 0.22 to 0.44 kg kg−1 and 1.14 to 1.38 Mg m−3, respectively. Factor combinations of these levels resulted in ranges of volumetric moisture contents from 0.27 to 0.54 m3 m−3, air filled porosity fractions from 0.27 to 0.00, and soil matric potentials from <‐1500 to near 0 kPa. The soil pCO2 contents measured from gas wells in pots increased from 0.16 to 1.64 cmol mol−1 as a result of decreased gas diffusion when the air‐filled porosity decreased. With increasing soil moisture, soil solution HCO‐3 concentrations attained values as high as 10 mM. Total chlorophyll (ChlT) contents of Anoka soybeans decreased as soil moisture, soil pCO2, and soil solution HCO‐3 increased. The same response was not observed in all calcareous soils. Calcareous soils with lower amounts of clay sized CaCO3, lower soil solution Mg2+, and/or lower bicarbonate extractable P did not produce as severe a chlorosis with increased moisture. This suggests that clay sized CaCO3, Mg, or P may be additional stress factors associated with HCO‐3 induced chlorosis. High soil moisture treatments on a calcareous soil provided an effective soil screen for differentiating between susceptible and nonsusceptible soybean cultivars. Soil temperatures of 12 and 26°C produced more chlorosis at a given soil moisture than at 16 or 19°C. Soil temperature appears to be an additional stress factor, which might explain some of the variability in the incidence of Fe chlorosis under field conditions.
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