High Mineralization Research Articles

Assessment of groundwater quality using hydrochemical process, GIS and multivariate statistical analysis at central Rif, North Morocco

This study investigated groundwater in the central Rif region of northern Morocco by analysing 55 water sampling points to assess its physicochemical and hydrogeochemical properties. Through hydrochemical analysis, GIS spatial exploration, and multivariate statistical analysis, a direct correlation was found between EC, TDS, and major ions, influencing overall water mineralization. The key findings included pH levels ranging from 6.10 to 8.52, EC from 828 to 4581 μS/cm, and varying concentrations of Ca2+, Mg2+, Na+, K+, HCO2–, Cl–, N–NO2–, and SO42–. Notably, TDS and TH ranged from 647.19–3609.36 mg/L and 64.23–1051.24 mg/L, respectively, with a significant portion of samples exceeding WHO guidelines, particularly chloride (61.81%), sulfate (92.72%), and nitrate (12.72%) samples. The Piper diagram highlights sodium chlorides (Na–Cl) as the predominant chemical facies (70.9%), while the Gibbs diagram emphasizes the impact of evaporation on water chemistry dynamics. This study revealed the complex influence of geological and anthropogenic factors on groundwater quality, potentially leading to seawater intrusion in coastal aquifers. The observed high mineralization and hardness levels, in addition to mild alkalinity, pose public health risks, underscoring the need for continuous monitoring and sustainable management practices in coastal groundwater management to protect human health and the environment.

Metal‐Ion and UVC Light‐Catalyzed Oxidation and Biodetoxification of Ponceau Xylidine in Aqueous Solution: A Comparative Study

AbstractMetal ion ( /oxidant) and UV light catalyzed (UV/oxidant) mineralization and biodetoxification of Ponceau Xylidine (PX) was undertaken in this study. Hydrogen peroxide (HP) and ammonium persulphate (APS) were employed as oxidants and and as metal ion catalysts. Although highest mineralization in /APS and UV/HP systems was exhibited at pH 1 (63.09 %) and 3 (64.83 %), respectively, lowest was shown by /oxidant systems at any pH. The Fenton process achieved mineralization of ≈48.50 % within the pH range of 3 to 6.15. The study established as a better catalyst than , and HP, a better oxidant (2.5 times more effective) than APS. Mineralization with UV/HP at 120 min is highest at pH 3 (92.0 %) and 6.15 (86.17 %). Various inorganic anions were found to inhibit mineralization. The maximum and minimum inhibition was shown by in UV/HP and UV/APS systems respectively. The inhibition effect of decreases with concentration till 0.01 mM, beyond which an increasing trend was observed. This study also demonstrates that the biodetoxification efficiency of various systems is in the order: UV/HP>UV/APS> /HP. Thus, UV/HP is the most suitable system for the treatment of PX, displaying a biodetoxification efficiency of 83.0 % and mineralization of 92.0 %.

Engineering an A-D-A structure to enhance internal electric fields in silica-PDI-decorated vertical graphene for efficient photocatalytic oxidation

Efficient charge separation and transfer are crucial for elevating photocatalytic oxidation, and designing a photocatalyst structure with strong internal electric fields (IEF) hold promise for addressing the issue. Herein, we synthesized a silica-PDI (silica-perylene diimides) decorated vertical graphene (s-PDI@VG) architecture with an Acceptor-Donor-Acceptor (A-D-A) structure. The A-D-A configuration facilitated electron transfer from tertiary N-atom to perylene tetracarboxylic dianhydride (PTCDA) and flower-like vertical graphene (VG), resulting in a larger molecular dipole moment of s-PDI@VG compared to PTCDA and s-PDI. Consequently, an IEF with 7.5 times higher than PTCDA and 4.8 times higher than s-PDI was built, which is beneficial for photogenerated charge separation. Additionally, the valence band (VB) of s-PDI@VG deepened from 1.43 eV to 1.62 eV, enabling it to afford a strong oxidation potential. As a proof-of-concept, the 3D s-PDI@VG photocatalyst demonstrated favorable persulfate (PMS) activation and pollutant adsorption, resulting in 1.6 times higher photogenerated holes based on LSCM results. This photocatalyst also achieved 98.2 % mineralization of high-ionization-potential (h-IP) organics within 30 min. This work offers insights into a novel photocatalytic oxidation strategy with high mineralization by engineering A-D-A structure and enhancing the IEF strength of photocatalyst molecules.

Characterizing bone density pattern and porosity in the human ossicular chain using synchrotron microtomography

The auditory ossicles amplify and transmit sound from the environment to the inner ear. The distribution of bone mineral density is crucial for the proper functioning of sound transmission as the ossicles are suspended in an air-filled chamber. However, little is known about the distribution of bone mineral density along the human ossicular chain and within individual ossicles. To investigate this, we analyzed fresh-frozen human specimens using synchrotron-based phase-contrast microtomography. In addition, we analyzed the volume and porosity of the ossicles. The porosity for the auditory ossicles lies, on average, between 1.92% and 9.85%. The average volume for the mallei is 13.85 ± 2.15 mm3, for the incudes 17.62 ± 4.05 mm3 and 1.24 ± 0.29 mm3 for the stapedes. The bone density distribution showed a similar pattern through all samples. In particular, we found high bone mineralization spots on the anterior crus of the stapes, its footplate, and along areas that are crucial for the transmission of sound. We could also see a correlation between low bone mineral density and holey areas where the bone is only very thin or missing. Our study identified a similar pattern of bone density distribution within all samples: regions exposed to lower forces generally show higher bone density. Further, we observed that the stapes shows high bone mineral density along the anterior crus and its footplate, which may indicate its importance in transmitting sound waves to the inner ear.

Assessment of water supply to the east European arctic agglomeration from groundwater, taking into account their quality and health risks

The purpose of the study is to assess the possibilities of using groundwater for water supply in the East European Arctic agglomeration based on an assessment of their quality and health risks. For this purpose, high-precision determinations of the complete macro- and microcomponent composition were carried out in sixty-six water samples taken from wells up to 180 m deep. It was found that in some samples the concentrations of Na+, Fe, B, Ba, Mn and U exceeded WHO standards. The least mineralized young waters are characterized by the processes of dissolution of carbonates with the transition of Ca, Mg, Ba, Sr into water, and the processes of leaching of Fe and Mn by acidic swamp waters from near-surface sediments. Waters of high mineralization, enriched in Na+, Cl-, B, Mo, Cd, Pb, were formed as a result of the dissolution of aluminosilicate rocks over thousands of years and mixing with relics of ancient and modern marine transgressions. An assessment of the average Water Quality Index value of the studied aquifer showed that, in general, the water is of excellent quality. Non-carcinogenic risks were determined primarily by uranium concentrations. The average danger index values for this element for children were 1.22. In adults it was slightly lower and amounted to 0.83. Carcinogenic risks are associated primarily with arsenic concentrations. The average total carcinogenic risk associated with this element was 3.8.10−5, which is acceptable, but samples from two wells showed total carcinogenic risk values above 10−4, which is in the high-risk area. For drinking purposes, it is preferable to use low-mineralized water with a minimum content of toxic elements. If necessary, preliminary aeration of the water is possible, during which precipitation of iron, arsenic and uranium occurs. Due to the typical nature of the problem under consideration for the Arctic regions, the results obtained can be used at other sites in the Subpolar zone.

An enhanced tri-layer bionic periosteum with gradient structure loaded by mineralized collagen for guided bone regeneration and in-situ repair

Severe fracture non-union often accompanied by damaged or even absent periosteum remains a significant challenge. This paper presents a novel tri-layer bionic periosteum with gradient structure and mineralized collagen (MC) mimics natural periosteum for in-situ repair and bone regeneration. The construct with ultrasonic polylactic acid as the loose outer fibrous layer (UPLA), poly(ε-caprolactone) as the intermediate barrier layer (PCL-M), and poly(ε-caprolactone)/MC as the inner osteoblastic layer (PM) was prepared. The physicochemical properties of layers were investigated. UPLA/PCL-M/PM exhibited a tensile strength (3.55 ± 0.23 MPa) close to that of natural periosteum and excellent adhesion between the layers. In vitro experiments demonstrated that all layers had no toxicity to cells. UPLA promoted inward growth of mouse fibroblasts. PCL-M with a uniform pore size (2.82 ± 0.05 μm) could achieve a barrier effect against fibroblasts according to the live/dead assay. Meanwhile, PM could effectively promote cell migration with high alkaline phosphatase expression and significant mineralization of the extracellular matrix. Besides, in vivo experiments showed that UPLA/PCL-M/PM significantly promoted the regeneration of bone and early angiogenesis. Therefore, this construct with gradient structure developed in this paper would have great application potential in the efficient and high-quality treatment of severe fractures with periosteal defects.

N, S co-doped porous carbon derived from waste medical masks to support LaFexCo1-xO3 as highly effective peroxymonosulfate catalysts for degradation of tetracycline

N and S co-doped porous carbon was prepared by decorating the waste disposable medical masks (WDMM) with thioacetamide (TAA) as the N/S source and then carbonizing at high temperature, which was subsequently incorporated Co-doped LaFeO3 to obtain NSC@LaFexCo1-xO3 catalysts. The resulting catalysts were used to construct an advanced oxidation process (AOP) based on activation of peroxymonosulfate (PMS) to degrade tetracycline (TC) in water. The physicochemical properties of the as-obtained catalysts were characterized and environmental factors affecting the catalytic performances of NSC@LaFexCo1-xO3/PMS were systematically investigated. The results showed that N and S co-doped porous carbon derived from thioacetamide (TAA)-functionalized WDMM at high temperature could be used as good support for LaFexCo1-xO3. The synergistic effects between N/S co-doped carbon and polyvalent metals (Co2+/Co3+ and Fe3+/Fe2+) endowed NSC@LaFexCo1-xO3 with enhanced catalytic performances, in which nearly 100% of TC could be removed by NSC@LaFe0.95Co0.05O3/PMS system within 10 min under the conditions of 0.2 g/L of catalyst dose, 2.5 mmol/L of PMS concentration and 10 mg/L of initial TC concentration. Furthermore, the constructed NSC@LaFe0.95Co0.05O3/PMS system exhibited a wider pH adaptability (3.0 ∼ 10.0), good salt resistance and reusability as well as high mineralization. The mechanisms and possible pathways of TC degradation by NSC@LaFe0.95Co0.05O3/PMS system were proposed and the ecotoxicity of TC and its intermediates was also evaluated. This study demonstrated the feasible transformation of waste masks into porous carbon with high added value for water pollution control and remediation, accomplishing the dual purposes of recycling and resource utilization of waste masks and protecting the environment.

Electrothermal mineralization of per- and polyfluoroalkyl substances for soil remediation

Per- and polyfluoroalkyl substances (PFAS) are persistent and bioaccumulative pollutants that can easily accumulate in soil, posing a threat to environment and human health. Current PFAS degradation processes often suffer from low efficiency, high energy and water consumption, or lack of generality. Here, we develop a rapid electrothermal mineralization (REM) process to remediate PFAS-contaminated soil. With environmentally compatible biochar as the conductive additive, the soil temperature increases to >1000 °C within seconds by current pulse input, converting PFAS to calcium fluoride with inherent calcium compounds in soil. This process is applicable for remediating various PFAS contaminants in soil, with high removal efficiencies ( >99%) and mineralization ratios ( >90%). While retaining soil particle size, composition, water infiltration rate, and cation exchange capacity, REM facilitates an increase of exchangeable nutrient supply and arthropod survival in soil, rendering it superior to the time-consuming calcination approach that severely degrades soil properties. REM is scaled up to remediate soil at two kilograms per batch and promising for large-scale, on-site soil remediation. Life-cycle assessment and techno-economic analysis demonstrate REM as an environmentally friendly and economic process, with a significant reduction of energy consumption, greenhouse gas emission, water consumption, and operation cost, when compared to existing soil remediation practices.

Efficient degradation of sulfadiazine by UV-triggered electron transfer on oxalic acid-functionalized corn straw biochar for activating peroxyacetic acid: Performance, mechanism, and theoretical calculation

A novel UV/oxalic acid functionalized corn straw biochar (OCBC)/peroxyacetic acid (PAA) system was built to degrade sulfadiazine from waters. 94.7 % of SDZ was removed within 30 min by UV/OCBC/PAA. The abundant surface functional groups and persistent free radicals (PFRs) on OCBC were responsible for these performances. Cyclic voltammetry (CV) and other characterization analysis revealed, under UV irradiation, the addition of OCBC served as electron donor, which might promote the reaction of electrons with PAA. The quenching and electron paramagnetic resonance (EPR) tests indicated that R-O•, 1O2 and •OH were generated. Theoretical calculations indicated sulfonamide bridge was vulnerable under the attacks of reactive species. In addition, high removal effect achieved by 5 reuse cycles and different real waters also suggested the sustainability of UV/OCBC/PAA. Overall, this study provided a feasible approach to remove SDZ with high mineralization efficiency, in addition to a potential strategy for resource utilization of corn straw.

Improved peracetic acid activation under simulated solar-light irradiation by regulating shells of Cu2O: In-situ photothermal activation

Advanced oxidation processes based on light-mediated peracetic acid (PAA) activation are novel degradation systems for wastewater purification. In this work, different shells of Cu2O are controllably synthesized and used to activate PAA under simulative solar light. Interestingly, the kobs in the system without temperature control is 2.47 times that in the temperature-controlled system, implying the important role of the photothermal conversion effect in PAA activation. Reactive oxygen species identification demonstrates that photothermal promotes the decomposition of PAA into radical OH with higher redox potential instead of R–O, which together with non-radical 1O2 achieves a high mineralization rate (72.49%). The core–shell structure enhances light absorption and photogenerated charge separation of Cu2O, thereby improving photothermal conversion efficiency. Meantime, the in-situ photothermal conversion effect expedites the diffusion of ROS and the cycle of active Cu(Ⅱ)/Cu(Ⅰ) ultimately achieves the high-efficiency and long-lasting PAA activation. This work provides novel strategies for solar energy utilization and PAA activation processes in wastewater purification.

Identification of hydrochemical processes of groundwater in Nekor-Ghiss plain (Morocco): using the application of multivariate statistics and Geographic Information Systems (GIS) to map groundwater

In arid and semiarid zones, groundwater is a vital and indispensable natural resource. Indeed, these water resources have become extremely limited due to several factors, including climate change, salinization, and overexploitation. This study covers the hydrochemical characterization of groundwater in the Nekor-Ghiss plain which is located in northern Morocco. To achieve this objective, a set of 79 water samples was analyzed, for various physical and chemical parameters of the samples, including E.C, pH, TDS, HCO3−, SO42−, Ca2+, Mg2+, Na+, K+, PO43−, NO3−, NO2− and NH4+. Several methods were used to interpret the hydrochemical data, namely graphical methods, principal component analysis (PCA), hierarchical ascending classification, and ion exchange indices. A detailed geochemical study of groundwater is described to identify the origin of the chemical composition of groundwater. The results show that the samples studied are characterized by very high mineralization (> 1500 mg/l), with the predominance of Na+, Cl− and SO42− ions. The interpretation of geochemical signatures of groundwater in the Nekor-Ghiss plain shows sulfate-sodium (Na–SO4) and chloride-sodium (Na–Cl) facies. Natural geochemical processes are responsible for water regulation, while human activities exert a limited influence on this control. Marine intrusion, evaporation, and the ion exchange process largely control the chemistry of the aquifer. Using PCA to confirm controlled water chemistry processes revealed three homogeneous groups. The study contributes to a better understanding of the quality and mineralization of groundwater in the Nekor-Ghiss plain, and it will serve as a reference for other plains with similar characteristics.

Mealworm Larvae Frass Exhibits a Plant Biostimulant Effect on Lettuce, Boosting Productivity beyond Just Nutrient Release or Improved Soil Properties

There is a need for alternatives or complements to synthetic fertilizers to enhance agricultural sustainability. Applying organic amendments can play a significant role in this. Insect droppings show high potential, though studies evaluating their agronomic value have only recently begun to emerge. This study compared black soldier fly (Hermetia illucens L.) and mealworm (Tenebrio molitor L.) larvae frass with another organic amendment (Nutrimais) derived from composting forestry, agro-industrial, and domestic waste. The experiment also included ammonium nitrate at two rates [the same as the organic amendments, 50 kg ha–1 nitrogen (N) (FullR), and half that rate (HalfR)] and an unfertilized control. The study spanned two growth cycles of lettuce (Lactuca sativa L.) grown in pots, followed by unfertilized oats (Avena sativa L.) to assess the residual effects of the fertilizing treatments. Mealworm larvae frass mineralized rapidly, with an apparent N recovery of 37.4% over the two lettuce growth cycles, indicating its high availability to soil heterotrophic microorganisms. The average dry matter yield (DMY) of lettuce was the highest among all treatments (12.8 and 9.8 g plant–1 in the first and second lettuce cycles), even compared to the FullR treatment (12.2 and 7.8 g plant–1), though without significant differences. Although mealworm larvae frass exhibited a high mineralization rate, the DMY cannot be attributed solely to N supply, as plants in the FullR treatment showed better N nutritional status. Mealworm larvae frass provided strong evidence of a plant biostimulant effect, not explained by the variables measured in this study. Black soldier fly larvae frass exhibited typical behavior of a moderately reactive organic amendment, while Nutrimais showed low reactivity, with a near-neutral mineralization/immobilization balance. The results suggest mealworm larvae frass is recommended for early maturing vegetable crops, whereas Nutrimais appears more suitable for perennial crops with low short-term nutrient requirements.

Distribution Profile of Benthic Macroinvertebrates in Some Rivers of Yaoundé City and Its Surroundings Using Self Organizing Map and Indicator value methods

Urban sprawl leads to the degradation of aquatic environments and, consequently, to the destruction of biodiversity. With the aim of highlighting the distribution profile of benthic macroinvertebrates in the city of Yaoundé and its surroundings according to the level of degradation, this study was carried out in seven rivers. A total of 144 taxa of benthic macroinvertebrates, belonging to 74 families, 15 orders, five classes, and three phyla, were collected from seven rivers in urban, peri-urban, and forest environments on Yaoundé and its surroundings. The self-organizing map (SOM) analysis tool was used to group the collected taxa from all stations into three clusters or affinity cores. The indicator value analysis (IndVal) method was employed to determine, based on their ecological preferences, which organisms were most likely to belong to each group. Out of the 144 collected taxa, only 44 were indicated to represent the three different groups. Thus, three communities were defined: the Hydropsyche community, with Hydropsyche sp. as the predominant taxon in Group III, characterizing well-oxygenated and low-mineralized stations; the Hydrocyrius community, where the species Hydrocyrius sp. predominates in Group I, describing stations with low oxygenation and moderate mineralization; and the Lumbriculidae community, where Lumbriculidae is the taxon associated with environments with high mineralization and critical oxygenation. These two methods contribute to the biomonitoring of tropical aquatic environments, firstly by grouping organisms by affinity and then identifying those that reflect the environment conditions. This facilitates the detection of changes in the quality of hydrosystems and guides management and conservation efforts.

Lithological Control to the Gold Mineralization in the Main Ridge Complex of the Bakan Gold District, Northern Sulawesi, Indonesia

Abstract Main Ridge complex is characterized by prominent high sulfidation epithermal-style mineralization with windows of porphyry style mineralization in the deeper zone. Several lithologic features play important controlling role in the occurrences of those mineralization. The oldest rock observed in the Main Ridge complex is unmineralized hornblende diorite (IDO-1) which encompasses the whole Bakan district. Several late intrusive stocks were concealed below the current topographic as mentioned as the microdiorite (IDO-2) and quartz diorite (IDO-3) in which the latter exhibits windows of porphyry-style mineralization. Those intrusive units were then covered by two unconformable volcanic tuff sequences which are andesitic tuff and dacitic tuff in compositions. Dacitic tuff is more favourable unit for gold mineralization compared to andesitic tuff which shown by near-surface ‘mushroom-like’ deposit form. In more peripheral area, the mineralization is also controlled by the bedding features of dacitic tuff which underlain by the less-mineralized andesitic tuff. In the latest stage, diatreme breccia cut all the lithologic units and becomes the most favourable host rock in sub-vertical deposit form. The comprehensive understanding of the lithological features is important to understand the mineralization control for further exploration target.

Experimental study on the scaling law of the heat exchange tube surface in the process of low-temperature single-effect distillation of high-mineralized mine water

High-mineralized mine water, due to its characteristics such as high mineralization degree, will have scaling phenomena on the surface of the heat exchange tube during the desalination treatment process, resulting in a reduction in the water production efficiency of the equipment and affecting the normal operation. To reduce the cost of water production and improve the service life of distillation equipment, this paper independently designs and builds a low-temperature single-effect distillation high-mineralized mine water experimental system to study the influence of operating parameters (temperature, spray density, concentration ratio, and system pressure) on the scaling law on the surface of the heat exchange tube. The distribution and characteristics of scale deposits on the surface of the heat exchange tube are analyzed through the growth rate and microscopic morphology of the scale deposit. The results indicate that the scaling on the surface of the titanium plate is mainly in the needle-like and rod-like morphology of CaCO3 aragonite, and a small amount of CaSO4 and calcite are generated with the increase of temperature. It was found that within the range of spray density from 0.0509 kg/(m·s) to 0.0625 kg/(m·s), concentration ratio from 2.3 to 2.7, and system pressure at 36.3 kPa, it is beneficial to the evaporation heat transfer of the heat exchange tube and the reduction of scaling generation. At the same time, it is found that the scaling of the first row of heat exchange tubes is mainly within the range of circumferential angle from 0 to 100 degrees, and the scaling of the second row and the third row of heat exchange tubes is mainly within the range of circumferential angle from 110 to 170 degrees. The research results of this paper provide support for the design and operation of the low-temperature multi-effect treatment system for mine water in engineering, promote the process of zero discharge of high-mineralized mine water in Xinjiang, China, and improve the resource utilization rate of high-mineralized mine water.

Biotic regulation of phoD-encoding gene bacteria on organic phosphorus mineralization in lacustrine sediments with distinct trophic levels

Organic phosphorus (Po) mineralization hydrolyzed by alkaline phosphatase (APase) can replenish bioavailable P load in the sediment water ecosystem of lakes. However, the understanding about the interaction between P load and bacteria community encoding APase generation in the sediment are still limited. Different P pools in the sediments from Taihu Lake, China were measured using sequential extraction procedure. The APAase activity (APA) were obtained accompanying with enzymatic dynamical parameters Vmax and Km. The abundances and diversity of gene phoD-harboring bacterial communities were assessed using high throughput sequencing. The analysis results showed the decrease of potentially bioavailable P fractions including MgCl2-P and Fe-P along sampling gradient southwards together with active P concentrations in the water. Conversely, increasing APA and absolute abundance of phoD gene were found with the decreasing of P loads southwards. Positive correlation (p < 0.05) between absolute abundance and APA indicated that phoD-encoding bacteria manipulated the APA and Po mineralization. Negative correlation (p < 0.01) suggested that the APA was restrained by high P load and was promoted under low P condition. However, higher Vmax and Km values suggested that high mineralization potential of Po maintained the high concentrations of potentially bioavailable P even the APA was restricted. The abundance increase of predominant genus Cobetia (from 15.51 to 24.34 %) mirrored by the reduced Calothrix abundance (from 24.65 to 1036 %) was speculated to be responsible for the APA promotion under low P condition. Higher diversity indices in the high P scenario suggested that high P load stimulated the ecological diversity of gene phoD-encoding bacteria community. Generally, rare taxa such as Burkholderia having high connected degrees in bacterial communities together with abundant genera synergistically manipulated the phoD gene abundance and APase generation. Interaction between P fractions and bacteria encoding phoD gene determined the eutrophication status in the lacustrine ecosystem.

Water Geochemistry and Inorganic Quality: Case of the Lower Rusizi Plain Aquifer, North-Western Burundi

The present study area is an alluvial plain consisting of fine clayey sand and coarse sand with mixed lithology. In September 2018, a water sampling campaign was carried out in 28 wells spread all over the plain. The interpretation of the results from the physicochemical analysis allows determination of the hydrogeochemical behaviour and quality of the groundwater sampled in this plain. The spatial distribution of physical and chemical parameters reveals a clear demarcation between the east and the west of the study area. Water samples from wells located in the western part of the plain show a high mineralisation with high electrical conductivity and total dissolved solids. Water samples from the southern and eastern parts show intermediate to low mineralisation. Graphical representations of chemical analysis in the Piper diagrams reveal that the groundwater in the study area can be classified into five families. The evaluation of water quality shows that concentration values of some ions like Na+, Ca2+, Mg2+, Cl−, HCO3− and SO42− are generally high in the western part of the plain along the Rusizi River with very high values particularly observed in the Rusizi delta.

MiRNA-seq analysis of high glucose induced osteoblasts provides insight into the mechanism underlying diabetic osteoporosis

The present study aims to explore the etiology of Diabetic osteoporosis (DOP), a chronic complication associated with diabetes mellitus. Specifically, the research seeks to identify potential miRNA biomarkers of DOP and investigated role in regulating osteoblasts. To achieve this, an animal model of DOP was established through the administration of a high-sugar and high-fat diet, and then injection of streptozotocin. Bone microarchitecture and histopathology analysis were analyzed. Rat calvarial osteoblasts (ROBs) were stimulated with high glucose (HG). MiRNA profiles of the stimulated osteoblasts were compared to control osteoblasts using sequencing. Proliferation and mineralization abilities were assessed using MTT assay, alkaline phosphatase, and alizarin red staining. Expression levels of OGN, Runx2, and ALP were determined through qRT-PCR and Western blot. MiRNA-sequencing results revealed increased miRNA-702-5p levels. Luciferase reporter gene was utilized to study the correlation between miR-702-5p and OGN. High glucose impaired cell proliferation and mineralization in vitro by inhibiting OGN, Runx2, and ALP expressions. Interference with miR-702-5p decreased OGN, Runx2, and ALP levels, which were restored by OGN overexpression. Additionally, downregulation of OGN and Runx2 in DOP rat femurs was confirmed. Therefore, the miRNA-702-5p/OGN/Runx2 signaling axis may play a role in DOP, and could be diagnostic biomarker and therapeutic target for not only DOP but also other forms of osteoporosis.

Buffer-free ozone-ferrate(VI) systems for enhanced oxidation of electron-deficient contaminants: Synergistic enhancement effects, systematic toxicity assessment, and practical applications

The combination of ozone (O3) and ferrate (Fe(VI)) oxidation technology demonstrates substantial potential for practical applications, though it has been underreported, resulting in gaps in comprehensive activity assessments and thorough exploration of its mechanisms. This study reveals that the previous use of a borate buffer solution obscured certain synergistic reactions between O3 and Fe(VI), causing a reduction of activity by ∼40 % when oxidizing the electron-deficient pollutant atrazine. Consequently, we reassessed the activity and mechanisms using a buffer-salt-free O3/Fe(VI) system. Our findings showed that the hydroxyl radical (·OH) served as the predominant active species, responsible for an impressive 95.9 % of the oxidation activity against electron-deficient pollutants. Additional experiments demonstrated that the rapid production of neglected and really important superoxide radicals (·O2-) could facilitate the decomposition of O3 to generate ·OH and accelerate the reduction of Fe(VI) to Fe(V), reactivating O3 to produce ·OH anew. Intriguingly, as the reaction progressed, the initially depleted Fe(VI) was partially regenerated, stabilizing at over 50 %, highlighting the significant potential of this combined system. Moreover, this combined system could achieve a high mineralization efficiency of 80.4 % in treating actual coking wastewater, complemented by extensive toxicity assessments using Escherichia coli, wheat seeds, and zebrafish embryos, showcasing its robust application potential. This study revisits and amends previous research on the O3/Fe(VI) system, providing new insights into its activity and synergistic mechanisms. Such a combined technology has potential for the treatment of difficult-to-degrade industrial wastewater.

Monitoring Photo-Fenton and Photo-Electro-Fenton process of contaminants emerging concern by a gas diffusion electrode using Ca10-xFex-yWy(PO4)6(OH)2 nanoparticles as heterogeneous catalyst

The catalytic performance of modified hydroxyapatite nanoparticles, Ca10-xFex-yWy(PO4)6(OH)2, was applied for the degradation of methylene blue (MB), fast green FCF (FG) and norfloxacin (NOR). XPS analysis pointed to the successful partial replacement of Ca by Fe. Under photo-electro-Fenton process, the catalyst Ca4FeII1·92W0·08FeIII4(PO4)6(OH)2 was combined with UVC radiation and electrogenerated H2O2 in a Printex L6 carbon-based gas diffusion electrode. The application of only 10 mA cm−2 resulted in 100% discoloration of MB and FG dyes in 50 min of treatment at pH 2.5, 7.0 and 9.0. The proposed treatment mechanism yielded maximum TOC removal of ∼80% and high mineralization current efficiency of ∼64%. Complete degradation of NOR was obtained in 40 min, and high mineralization of ∼86% was recorded after 240 min of treatment. Responses obtained from LC-ESI-MS/MS are in line with the theoretical Fukui indices and the ECOSAR data. The study enabled us to predict the main degradation route and the acute and chronic toxicity of the by-products formed during the contaminants degradation.