Two monoclonal antibodies were used to reveal the nature and distribution of pectins in cell walls and in the secretion of the style inBrugmansia (Datura) suaveolens at the light and electron microscope level. The antibodies JIM 5 and JIM 7 distinguish between unesterified and methylesterified pectins. Unesterified pectins occur in the walls of both transmitting tissue and cortex. The high methylesterified pectin is limited to cell walls in the cortex. The intercellular substance contains only unesterified pectins.
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