High Levels Of Stimulation Research Articles

The Effect of the CIRC and Learning Motivation on Reading Comprehension Skills for Junior High School Students

This study aimed to analyze how the CIRC learning model and learning motivation affected students' reading comprehension abilities. This study uses a 2x2 factorial design and a quasi-experimental methodology. A reading comprehension test and a questionnaire about learning motivation were employed in the study. A total of 24 people from each group were used to create the research sample. Data analysis was done descriptively. The results showed that CIRC influenced students' reading comprehension results. The experimental class (CIRC) students had a greater reading comprehension level than those in the control group (conventional). The reading comprehension skills of students with high learning motivation (CIRC) differ significantly from those of pupils with low learning motivation (traditional). The reading comprehension skills of students with low learning motivation (CIRC) differ considerably from those of pupils with high levels of stimulation (conventional). The CIRC model interacts with learning motivation to influence students' reading comprehension skills. Students with high reading interest (CIRC) can improve their ability to read and understand explanatory texts and vice versa.

RELATED OUTCOMES OF STRENGTH TRAINING IN CHILDREN AND ADOLESCENTS: A CONTEMPORARY REVIEW OF THE RELEVANT LITERATURE

Muscle strength is an essential element of any movement, whether in sport or in daily activity. However, despite its trainability, an aspect often studied in adults, the same is not verified in the strength training development in youth. Currently, there is a wealth of information available, often contradictory, about what is safe or appropriate for pre-and post-puberty strength training and development. In this point of view, we will briefly discuss some of the current and relevant literature and provide evidence-based practical recommendations for resistance exercise training in children and adolescents, according to international recommendations. The results revealed that the child is, from a biological and metabolic point of view, a non-specialized organism, where adaptations occur without the need for a high level of stimulation. Recent data indicate that resistance training programs, well-structured and appropriately prescribed and supervised by trained specialists, are safe and provide unique benefits for youth, such as increased strength and muscle power, reduced cardiovascular risk, helps weight control, strengthens bones, increases psychosocial well-being, improves motor performance skills, and may reduce sports-related injuries, and should be viewed as an essential component of preparatory training programs for aspiring young athletes. Current recommendations suggest that school-aged youth should participate daily in 60 minutes or more of moderate to vigorous physical activity that is safe, effective, and enjoyable. Regular participation in a variety of physical activities during childhood and adolescence can support and encourage participation in physical activity as an ongoing lifestyle choice later in life.

Active Middle Ear Implant Evoked Auditory Brainstem Response Intensity-Latency Characteristics.

ObjectiveTo analyze intensity-latency functions of intraoperative auditory evoked brainstem responses (ABRs) to stimulation by the Vibrant Soundbridge (VSB) active middle ear implant with respect to coupling efficiency, VSB evoked ABR thresholds, and coupling modality [oval window (OW) placement vs. Incus placement and vs. round window (RW) placement].Study DesignExploratory study.SettingBi-centric study at tertiary referral centers.PatientsTwenty-four patients (10 female, 14 male, mean age: 58 years) who received a VSB.Outcome MeasuresWave-V intensity-latency functions of intraoperative VSB evoked ABRs using a modified audio processor programmed to preoperative bone conduction thresholds for stimulation. Threshold level correction to coupling efficiency and ABR thresholds. Individual plots and exponential function fits.ResultsAfter ABR threshold level correction, the latency functions could be aligned. A large variance of latencies was observed at individual threshold level. Wave-V latency was longest in the Incus placement subgroup (9.73 ms, SD: 1.04) as compared to OW placement subgroup (9.47 ms, SD: 1.05), with the shortest latency in the RW placement subgroup (8.99 ms, SD: 0.68). For increasing stimulation levels, the variance decreased with intensity-latency function slopes converging toward a steady-state (saturation) latency caused by saturation of audio processor (stimulation) gain. Latency saturation was reached at a stimulation level of 50 dB nHL for the OW placement subgroup, 35 dB nHL for the Incus placement subgroup, and 30 dB nHL for the RW placement subgroup. The latency and saturation results indicated decreased dynamic range for RW placement, i.e., reverse stimulation.ConclusionsVSB evoked ABR wave-V intensity-latency function slopes were similar to acoustic stimulation at high stimulation levels with a shift toward longer latencies caused by audio processor signal delay. Saturation of latencies occurred for higher stimulation levels due to saturation of audio processor gain. Thus, the analysis of VSB evoked intensity-latency functions appears to allow for the objective assessment of a patient's individual dynamic range. This can further improve diagnostics as well as intraoperative and postoperative quality control.

Welcoming autistic students to academic libraries through innovative space utilization

“If the library can serve as a safe and welcoming place for these students, it will help contribute to their ultimate college success.” (Anderson, 2018). Certain characteristics of autism spectrum disorder can make it challenging for autistic college students to make use of academic libraries. High levels of sensory stimulation and the need to understand and comply with neurotypical social norms can make library spaces feel unwelcoming and difficult to use. West Chester University Libraries decided to develop a dedicated space for our growing cohort of autistic students to study. This article will discuss the reasons why autistic students may find academic libraries challenging as well as the considerations and process of developing a separate space for autistic students within a library.

The effect of Kagocel® on gene expression of Toll-like receptors of innate immunity in THP-1 human monocytes with different levels of differentiation

Kagocel® is used in Russia for the treatment of viral infections. In terms of its chemical structure, Kagocel® active ingredient is a copolymer of gossypol polyphenol and carboxymethylcellulose. The study investigated antiviral and cytokine-inducing activity of Kagocel®, as well as its toxic effects. The aim of the study was to investigate the effect of Kagocel® active ingredient on the induction of expression of the innate immune system receptor genes (Toll-like receptors, TLR) in the THP-1 human acute monocytic leukemia cell line with different levels of differentiation. Materials and methods: the effect of Kagocel active ingredient was investigated at the concentrations of 0.2 and 2 mg/mL in the THP-1 human acute monocytic leukemia cell line with different levels of differentiation: non-differentiated monocytes, and monocytes differentiated into macrophage-like cells. Comparative analysis of the activity of TLR 2, 3, 4, 7, 8, 9 genes was carried out by quantitative RT-PCR. The study determined standard deviations of the levels of gene expression in the experimental cells (2deltaCq ± SD) relative to the expression in the control cells. Results: Kagocel active ingredient at the concentration of 0.2 mg/mL induced activation of TLR2 expression in THP-1 monocytes by 3.5 times, TLR3 by 2 times, TLR4 by 1.6 times, and at the concentration of 2 mg/mL also induced activation of TLR7 and TLR8 by 1.4 times, and TLR9 by 2 times. The levels of TLR2, TLR3, TLR9 induction were significantly higher in THP-1 monocytes partially differentiated into macrophage-like cells, and the highest stimulation level was observed for TLR2 (8 times). Conclusions: the results obtained characterise Kagocel® as a stimulator of TLR genes in the THP-1 cell line. The number of TLR genes induced in THP-1 monocytes was shown to increase with the increase in the product concentration. THP-1 monocyte differentiation into macrophage-like cells enhances susceptibility to Kagocel®. The positive regulation of TLR genes activity may account for antiviral and interferon-inducing properties of Kagocel®, and also suggests the possibility of expanding the use of the product for various immune-associated diseases.

Sensitive Physiological Indices of Pain Based on Differential Characteristics of Electrodermal Activity.

Electrodermal activity (EDA) has been widely used to assess human response to stressful stimuli, including pain. Recently, spectral analysis of EDA has been found to be more sensitive and reproducible for assessment of sympathetic arousal than traditional indices (e.g., tonic and phasic components). However, none of the aforementioned analyses incorporate the differential characteristics of EDA, which could be more sensitive to capturing fast-changing dynamics associated with pain responses. We have tested the feasibility of using the derivative of phasic EDA and the modified time-varying spectral analysis of EDA. Sixteen subjects underwent four levels of pain stimulation using electric stimulation. Five-second segments of EDA were used for each level of stimulation, and pre-stimulation segments were considered stimulation level 0. We used support vector machines with the radial basis function kernel and multi-layer perceptron for three different scenarios of stimulation-level classification tasks: five stimulation levels (four levels of stimulation plus no stimulation); low, medium, and high pain stimulation (stimulation levels 0-1, 2, and 3-4, respectively); and high stimulation levels (stimulation levels 3-4) vs. no stimulation. The maximum balanced accuracies were 44% (five stimulation levels), 63% (for low, medium, and high pain stimulation), and 87% (sensitivity 83% and specificity 89%, for high stimulation vs. no stimulation). The differential characteristics of EDA contributed highly to the accuracy of pain stimulation level detection of the classifiers. The external validity dataset was not considered in the study. Our approach has the potential for accurate pain quantification using EDA.

Temporal characteristics of the cochlear response after noise exposure

The effect of intense noise on cochlear sensitivity has been extensively studied, but its influence on the temporal characteristics of the cochlear response is still unclear. This study investigated the effects of noise exposure on the latency of cochlear response and cochlear forward masking. Rats were exposed to an octave band noise (8–16 kHz) at 90 dB SPL for 5 days. Cochlear compound action potentials (CAPs) induced by single- and double-tone stimuli and distortion product otoacoustic emissions (DPOAE) were recorded 1 day or 2 months after the noise exposure. The latency of the CAP and its forward masking were compared between the noise-exposed rats and normal control rats. The noise exposure significantly reduced DPOAE and elevated CAP threshold in the noise band region, but not in the other areas. Even in the noise band area, the noise did not reduce CAP-amplitude at the high stimulation level (80 dB SPL). Correspondingly, about one-third of the outer hair cells (OHC) in the noise band area disappeared, while the inner hair cells (IHC) did not. However, the noise exposure in the frequency range of 4–24 kHz significantly prolonged CAP latency and increased its variability, while the CAP forward masking effect was significantly enhanced in the frequency range of 16–30 kHz. The frequency-dependent changes in CAP latency and forward masking after noise exposure may reflect different types of synaptic subinjury in the cochlea, which may lead to psychophysical consequences of sound localization and speech recognition.

Quality of early childcare in the home and cognitive development at age 5: results from the South African birth to Twenty Plus cohort study

ABSTRACT This study, using longitudinal data from the South African Birth to Twenty Plus study, assessed associations between early childcare quality in the home between six months and two years, household SES and cognitive development at age 5. Childcare quality was assessed using measures of maternal responsiveness and cognitive stimulation. Cognitive development at age 5 was assessed using the Revised Denver Pre-screening Developmental Questionnaire (R-DPDQ). Bivariate analysis and multiple linear regressions were conducted. The analytical sample comprised 856 mother–child pairs. Quality of care in the home differed significantly according to SES status, with more children in the low SES group receiving low quality of care. There was no association between childcare quality and cognitive development. There were differences in the cognitive development scores of children in different socio-economic groups; high levels of cognitive stimulation in the home were associated with increased R-DPDQ scores for children in the low SES group.

Minoxidil nanoparticles as potential intradermal carrier for H5 DNA vaccine delivery

Background: DNA formulations provide the basis for safe and cost-effective vaccine. Low efficiency is often observed in the delivery of DNA vaccines and to induce a strong and long-lasting immune response, effective DNA delivery technologies are required that can induce high and continued levels of antigen production and stimulation at appropriate target sites. Methods and materials: In order to develop a new strategy for intradermal delivery of DNA vaccine, a recombinant plasmid encoding hemagglutinin (HA) gene of avian influenza virus, was formulated using green synthesis of Minoxidil nanoparticles (Min/NP) to develop nanoencapsulated H5 DNA vaccine (Min/NP/H5). Min/NP was successfully synthesized and uniformly dispersed with sizes in the range of 22–50 nm with an average size of 32 nm and positive charge of 16 mV. Ex vivo intradermal delivery of Min/NP/H5 were analysed by using Parallel Artificial Membrane Permeability Assay (PAMPA) and qPCR. Results: The results showed 100% of nanoencapsulated H5 DNA vaccine passed the artificial membrane. BALB/c mice (10 weeks old-female and male) immunized once intradermally with 150 μl of Min/NP/H5 behind the ear showed no clinical manifestations. H5 plasmid DNA was successfully detected from the dermis of the vaccinated mice as early as 1 h post-vaccination. In addition, sera collected from mice immunized with Min/NP/H5 showed rapidly increasing antibody titres against H5 on day 14 after immunization. The highest average antibody titres were detected on day 35 post immunization at 89.6 ± 35.05. Conclusion: Hence, single intradermal administrations of Min/NP/H5 to mice was able to efficiently deliver DNA vaccine and induced high levels of the antibody.

Membrane Tension Can Enhance Adaptation to Maintain Polarity of Migrating Cells

Migratory cells are known to adapt to environments that contain wide-ranging levels of chemoattractant. Although biochemical models of adaptation have been previously proposed, here, we discuss a different mechanism based on mechanosensing, in which the interaction between biochemical signaling and cell tension facilitates adaptation. We describe and analyze a model of mechanochemical-based adaptation coupling a mechanics-based physical model of cell tension coupled with the wave-pinning reaction-diffusion model for Rac GTPase activity. The mathematical analysis of this model, simulations of a simplified one-dimensional cell geometry, and two-dimensional finite element simulations of deforming cells reveal that as a cell protrudes under the influence of high stimulation levels, tension-mediated inhibition of Rac signaling causes the cell to polarize even when initially overstimulated. Specifically, tension-mediated inhibition of Rac activation, which has been experimentally observed in recent years, facilitates this adaptation by countering the high levels of environmental stimulation. These results demonstrate how tension-related mechanosensing may provide an alternative (and potentially complementary) mechanism for cell adaptation.

Level coding by phase duration and asymmetric pulse shape reduce channel interactions in cochlear implants

Conventional loudness coding with CIs by pulse current amplitude has a disadvantage: Increasing the stimulation current increases the spread of excitation in the auditory nerve, resulting in stronger channel interactions at high stimulation levels. These limit the number of effective information channels that a CI user can perceive. Stimulus intensity information (loudness) can alternatively be transmitted via pulse phase duration. We hypothesized that loudness coding by phase duration avoids the increase in the spread of the electric field and thus leads to less channel interactions at high stimulation levels. To avoid polarity effects, we combined this coding with pseudomonophasic stimuli. To test whether this affects the spread of excitation, 16 acutely deafened guinea pigs were implanted with CIs and neural activity from the inferior colliculus was recorded while stimulating with either biphasic, amplitude-coded pulses, or pseudomonophasic, duration- or amplitude-coded pulses. Pseudomonophasic stimuli combined with phase duration loudness coding reduced the lowest response thresholds and the spread of excitation. We investigated the channel interactions at suprathreshold levels by computing the phase-locking to a pulse train in the presence of an interacting pulse train on a different electrode on the CI. Pseudomonophasic pulses coupled with phase duration loudness coding reduced the interference by 4-5% compared to biphasic pulses, depending on the place of stimulation. This effect of pseudomonophasic stimuli was achieved with amplitude coding only in the basal cochlea, indicating a distance- or volume dependent effect. Our results show that pseudomonophasic, phase-duration-coded stimuli slightly reduce channel interactions, suggesting a potential benefit for speech understanding in humans.

A stepwise approach investigating salivary responses upon multisensory food cues

Exposure to sensory food cues such as smell, vision, taste and/or texture may trigger anticipatory physiological responses such as salivation, participating on adequate metabolism of the signaled food. However, the individual contribution of each sensory modality as well as the impact of particular food products on salivation and salivary composition remains unclear. Therefore, by systematically varying sensory modalities and nutrient content of food stimuli, we investigated their effect on saliva secretion, α-amylase activity and other salivary characteristics (pH level, buffering capacity, MUC5B concentration, and total protein content). Over 3 sessions, 46 normal-weight healthy participants were exposed to 12 conditions, consisting of 4 levels of sensory stimulation (odor, odor + vision, odor + vision + taste, and odor + vision + taste + mastication) and 3 types of stimuli (bread, high-in-starch; cucumber, low-in-starch; and parafilm as non-food control) during which saliva was collected. Linear mixed models showed a significant increase in salivation with increasing levels of sensory stimulation. α-amylase secretion rate increased upon the highest level of stimulation, which involved mastication, compared to odor and odor + visual level of stimulation. Other salivary characteristics varied with the level of sensory stimulation, which might be related to the total volume of salivation. The type of stimuli did not influence the saliva composition (α-amylase concentration nor other salivary components). Our findings indicate that cumulative sensory information, rather than specific (food) product, play a vital role in anticipatory salivary responses.

Cognitive Stimulation at Home and in Child Care and Children's Preacademic Skills in Two-Parent Families.

This paper used the Early Childhood Longitudinal Study-Birth Cohort (N = 1,258) to examine the influence of hilevels of cognitive stimulation from mothers, fathers, and childcare providers at 24 months and children's pre-academic skills at 48 and 60 months in two parent families. Results from path analysis showed direct positive effects of fathers' early cognitive stimulation on early reading and math skills at 48 and 60 months. There were also two moderated effects: The effects of high levels of maternal stimulation at 24 months on early math and reading skills at 48 months were largest for children also receiving high levels of cognitive stimulation from their childcare providers. Implications for including fathers in studies of the home cognitive stimulation and strengthening the parent-childcare connection are discussed.

Maternal, paternal, and other caregivers' stimulation in low- and- middle-income countries.

Globally, studies have shown associations between maternal stimulation and early child development. Yet, little is known about the prevalence of paternal and other caregivers' stimulation practices, particularly in low- and- middle-income countries (LMICs). Data from the Multiple Indicators Cluster Survey (MICS) and the Demographic and Health Survey (DHS) were combined across 62 LMICs (2010-2018). The sample included 205,150 mothers of children aged 3 and 4 years. High levels of stimulation were defined as caregiver engagement in at least 4 out of 6 possible activities with the child. The proportion of mothers, fathers, and other caregivers providing high levels of stimulation was calculated by country, region, and for the whole sample. Socioeconomic disparities within and between countries were estimated. On average, 39.8% (95% CI 37.4 to 42.2) of mothers, 11.9% (95% CI 10.1 to 13.8) of fathers, and 20.7% (95% CI 18.4 to 23.0) of other adult caregivers provided high levels of stimulation. Stimulation varied by region, country income group, and Human Development Index (HDI), with higher levels of maternal and paternal-but not other caregivers'-stimulation in high-income and high-HDI countries. Within countries, stimulation levels were, on average, lower in the poorest relative to the richest households, and some but not all countries exhibited differences by child sex (i.e., boys vs. girls) or area (i.e., urban vs. rural). Results suggest a need for intervention efforts that focus on increasing caregiver stimulation in LMICs, particularly for fathers and in low-income contexts.

Breakdown of primary frequencies used in pediatric hearing screening with distortion-product otoacoustic emissions (DPOAEs)

Background DPOAEs are acoustic energy within the external auditory canal (EAC) arising from the non-linear interaction within the cochlea; of 2 pure tones presented simultaneously; of primary frequencies f1 and f2. DPOAEs are recorded in nearly any normal-hearing subject and in patients with hearing loss up to 50 dBHL while recordings may be deceiving in the presence of severe to profound hearing loss.Objective To determine the intensity levels of f1 and f2 at which DPOAEs truly reflect the active metabolic processes within the cochlea.Setting University HospitalStudy ProspectiveMethods One-hundred and forty ears were categorized into three groups; A (n = 50), B (n = 50) and C (n = 40); according to subjects' age (12 to 18, 2 to 12 and <2 years, respectively). A fourth group D was included; which entailed recordings from a dummy cavity simulating a child’s external auditory canal. Each group was further subdivided into two subgroups according to hearing levels; either normal or severe to profound hearing loss. DP-grams were interpreted at 3 points/octave steps for all groups and reviewed as ‘Pass’ or ‘Fail’ according to set criteria. Stimulus levels were adjusted such that L1 exceeded L2 by 10 dB. Start levels for L1 and L2 were 80 and 70 dBSPL. Levels were reduced simultanueously in 5 dB steps for the following recordings; to reach 65 and 55 for the last recording.Results Using the higher three stimulation levels, all subgroups had ‘Pass’ DP grams. On using the last and lowest recording level, only subgroups of normal hearing ears had ‘Pass’ DP grams; while subgroups of ears with sensorineural hearing loss (SNHL) demonstrated ‘Failed’ DP grams at all frequencies.Conclusions For audiometric screening purposes, DPOAEs show to be rather insensitive to hearing loss at higher stimulation levels and may reveal false positive results.

The effects of environmental enrichment on skin barrier recovery in humans: a randomised trial

This study investigated whether environmental enrichment (EE) could reduce stress and improve wound healing in humans. 120 participants underwent a standardised tape-stripping procedure and were then randomised to interact for 30 minutes with one of three EE interventions (comfort blankets as tactile enrichment, music as auditory enrichment or a Paro robot as multi-sensory enrichment) or to a control group. Skin barrier recovery (SBR) was measured using transepidermal water loss at baseline, after tape-stripping and after the intervention. Psychological variables, cortisol and alpha-amylase were measured at the three time-points. SBR did not significantly differ between the EE conditions and the control condition. The music condition had higher stimulation levels than the control condition, and the comfort condition had significantly lower relaxation levels than the control condition after the intervention. The EE interventions tested were not beneficial for wound healing compared to a control group. Limitations were that the sample were not stressed and an active control condition was used.

Scientific and methodical fundamentals of the economic development strategy of the country for the near future

The key points of modernization of the national economy are identified: widespread development of market infrastructure, minimization of direct government intervention; cooperation and integration of interested business entities and the creation of cooperative integration corporations (firms) and cluster systems; end-to-end export orientation of domestic agribusiness; a high level of labor stimulation and its direct connection with production results.

The relationship between maximum tolerance and motor activation during transcutaneous spinal stimulation is unaffected by the carrier frequency or vibration.

Transcutaneous spinal stimulation (TSS) is a useful tool to modulate spinal sensorimotor circuits and has emerged as a potential treatment for motor disorders in neurologically impaired populations. One major limitation of TSS is the discomfort associated with high levels of stimulation during the experimental procedure. The objective of this study was to examine if the discomfort caused by TSS can be alleviated using different stimulation paradigms in a neurologically intact population. Tolerance to TSS delivered using conventional biphasic balanced rectangular pulses was compared to two alternative stimulation paradigms: a 5 kHz carrier frequency and biphasic balanced rectangular pulses combined with vibrotactile stimulation. In ten healthy participants, tolerance to TSS was examined using both single‐pulse (0.2 Hz) and continuous (30 Hz) stimulation protocols. In both the single‐pulse and continuous stimulation protocols, participants tolerated significantly higher levels of stimulation with the carrier frequency paradigm compared to the other stimulation paradigms. However, when the maximum tolerable stimulation intensity of each stimulation paradigm was normalized to the intensity required to evoke a lower limb muscle response, there were no statistical differences between the stimulation paradigms. Our results suggest that, when considering the intensity of stimulation required to obtain spinally evoked motor potentials, neither alternative stimulation paradigm is more effective at reducing discomfort than the conventional, unmodulated pulse configuration.

Neolymphogenesis and Immunogistogochemical Study of Lymph Nodes in Breast Cancer

Background. Our previous studies have shown that postnatally formed lymph nodes (PNFLN) can serve as a source of biological signals activating antitumour immune reactions and suppressing the spread of metastatic malignant cells.Aim. To determine the expression of CD3, CD20, CD68 in native, sentinel and postnatally induced lymph nodes of the axillary zone in breast cancer.Materials and methods. The study involved an analysis of digitalized images of the immunohistochemical expression of a fixed panel of antibodies CD3, CD20, CD68. The expression levels were assessed quantitatively by counting the expressed cells in each studied node for four main structural and functional zones.Results and Discussion. The results of a comparative immunohistochemical study of native, sentinel and postnatally induced lymph nodes showed that the content of CD3, CD20, CD68 demonstrates fundamental differences in different lymph node structures.Сonclusions1. In postnatally induced lymph nodes, compared to native and sentinel lymph nodes, the distinct expression of antibodies to the main immunocompetent cells, which realize key immune responses in the lymph node, can indicate an increased functional status of the newly formed lymph nodes. 2. The study demonstrated a high level of antigenic stimulation of T and B lymphocytes in postnatally induced lymph nodes, as well as indicated a possible role of macrophage cells in the stimulation of neolymphogenesis and the formation of new lymph nodes. 3. The study provides the basis for further research into postnatal induced lymph nodes in cancer patients.

Vascular endothelium damage from catheter-induced mechanical stimulation causes catheter sleeve formation in a rabbit model

Intravenous catheters are widely used but are often removed due to complications associated with catheter sleeve formation. A catheter sleeve can develop from a thrombus, and catheter-induced vascular endothelium damage may be a critical factor for thrombus formation. We investigated the effect of catheter-induced mechanical stimulation on venous endothelial cells and catheter sleeve formation and the efficacy of anti-thrombogenic technology for preventing catheter sleeve formation in vivo. We surgically implanted poly(2-methoxyethyl acrylate)-coated and uncoated catheters with and without a stylet into the right external jugular vein of a rabbit model for 14 days. Catheter sleeve formation and the ratio of residual venous endothelial cells were compared using histological examination and immunostaining with an anti-CD31 antibody, respectively. Stiffening an uncoated catheter with a stylet induced catheter sleeve formation along more than two-thirds of the length of the catheter. The ratios of residual venous endothelial cells at the tip of uncoated catheters with and without a stylet were 3% and 36%, respectively. While poly(2-methoxyethyl acrylate) coating also reduced the ratio of venous endothelial cells at the tip of the stiffened catheter (12%), it prevented external thrombus and catheter sleeve formation. High levels of mechanical stimulation can affect catheter-related thrombosis and promote catheter sleeve formation, and anti-thrombogenic technology such as a poly(2-methoxyethyl acrylate) coating reduces thrombus formation and can prevent catheter sleeve formation on stiffened catheters. Further studies are required to determine the maximum degree of venous endothelial cell damage before catheter sleeve formation and to compare other anti-thrombogenic technologies with poly(2-methoxyethyl acrylate) for preventing catheter sleeve formation.