High Level Of Fidelity Research Articles

Ethnomedicinal uses of plants for various diseases in the remote areas of Changa Manga Forest, Pakistan.

This study aims at reporting the indigenous knowledge of the medicinal flora from the inhabitants of surroundings of the World's largest artificial planted forest "Changa Manga", Pakistan. Data were collected by direct interviews and group meetings from 81 inhabitants including 32 local healers having information regarding the use of indigenous medicinal plants over a period of one year. Different statistical tools were applied to analyze the data including Frequency citation (FC), Relative frequency citation (RFC), Use Value, Factor of informants consensus and fidelity level. This study reported 73 plant species belonging to 37 plant families and 46 genera. The majority of plant species belong to compositae family. The most commonly used medicinal plants were P. hysterophorus L., P. dactylifera L., S. indicum L, P. harmala L., P. emblica L., and A. indica A.Juss. The greatest number of species was used to cure gastrointestinal disorders. The highest fidelity level (68.18%) was of E. helioscopia to cure gastrointestinal disorders. Maximum fresh uses (17) were reported by C. dactylon (L.) Pars. While the highest number of species reporting fresh uses in similar number was 13. In this study, five novel plants are being reported for the first time in Pakistan for their ethnomedicinal worth. Our data reflect unique usage of the medicinal plants in the study area. The statistical tools used in the study proved useful in pointing the most important and disease category specific plants. High use value plant and the new reported medicinal plants might prove an important source of the isolation of pharmacologically active compounds.

A BAYESIAN NEURAL NETWORK APPROACH TO MULTI-FIDELITY SURROGATE MODELING

This paper deals with surrogate modeling of a computer code output in a hierarchical multi-fidelity context, i.e., when the output can be evaluated at different levels of accuracy and computational cost. Using observations of the output at low- and high-fidelity levels, we propose a method that combines Gaussian process (GP) regression and the Bayesian neural network (BNN), called the GPBNN method. The low-fidelity output is treated as a single-fidelity code using classical GP regression. The high-fidelity output is approximated by a BNN that incorporates, in addition to the highfidelity observations, well-chosen realizations of the low-fidelity output emulator. The predictive uncertainty of the final surrogate model is then quantified by a complete characterization of the uncertainties of the different models and their interaction. The GPBNN is compared to most of the multi-fidelity regression methods allowing one to quantify the prediction uncertainty.

Cell behaviors that pattern developing tissues: the case of the vertebrate nervous system.

Morphogenesis from cells to tissue gives rise to the complex architectures that make our organs. How cells and their dynamic behavior are translated into functional spatial patterns is only starting to be understood. Recent advances in quantitative imaging revealed that, although highly heterogeneous, cellular behaviors make reproducible tissue patterns. Emerging evidence suggests that mechanisms of cellular coordination, intrinsic variability and plasticity are critical for robust pattern formation. While pattern development shows a high level of fidelity, tissue organization has undergone drastic changes throughout the course of evolution. In addition, alterations in cell behavior, if unregulated, can cause developmental malformations that disrupt function. Therefore, comparative studies of different species and of disease models offer a powerful approach for understanding how novel spatial configurations arise from variations in cell behavior and the fundamentals of successful pattern formation. In this chapter, I dive into the development of the vertebrate nervous system to explore efforts to dissect pattern formation beyond molecules, the emerging core principles and open questions.

Nest box occupancy dynamics by hazel dormice (Muscardinus avellanarius) in central-western Romania

The hazel dormouse, Muscardinus avellanarius is a vulnerable and protected species that is challenging to study due to its specific way of life. Limited data on its distribution and population parameters are available for Romania. We collected data regarding species’ biology and ecology in the hilly areas of central-western Romania, within deciduous forests dominated by oak or beech species. We regularly monitored 80 wooden nestboxes, which served as shelter and breeding sites for the species, to assess the seasonal and sex-related variations in nest box usage. The highest occupancy levels were observed during the autumn, with a smaller peak in the spring. Males occupied more nest boxes than females, although the difference was not statistically significant. They also travelled greater distances. Most nest boxes were occupied by a single individual at a time. Recapture rates did not differ significantly between males and females, and most individuals were recaptured only once. Hazel dormice showed a high level of site fidelity, often returning to the same nestbox or one nearby. Interestingly, 14% of the females bred twice a year, which is a lower percentage compared to findings from other studies. Nest box occupancy rates in this region were also lower than those reported in other areas. However, it is important to note that these variations may be attributed to differences in methodology. The occupancy rate declined with the increased presence of the larger species, the fat dormouse Glis glis, which outcompeted the hazel dormouse.

Addressing the Patient Experience through Human- Centred Design: A Scoping Review

Background: There is an increasing interest in patient experience for improving the quality of services together with clinical effectiveness and patient safety. Human-Centred Design (HCD), (design thinking and service design) are design approaches that are increasingly used to propose new patient-centred solutions. However, the evidence on the relationship between HCD and patient experience is at an early stage.
 Objective: This study investigates the intersection between patient experience and HCD by focusing on patient-centred care models, HCD principles, and strategies.
 Methods: First, PRISMA was used for the scoping review which includes 45 articles that apply HCD to improve patient experience. Second, the articles were analysed following the PICOS (participants, intervention, output, study design) framework. Third, a qualitative analysis of the studies was performed following the ENTREQ method to identify patterns in the relationship between HCD and patient experience. We create a framework that defines design practices in terms of three strategies (understanding people, stakeholder involvement, and systems approach) and six principles (understand, involve, evaluate, iterate, holistic experience, and multidisciplinary team), intersecting with the eight patient experience dimensions (respect, coordination, information, comfort, emotional support, involvement, continuity, access). Main results: The use of HCD primarily addresses respect for patient preferences, coordination between professionals, adequate patient information and continuity of care. 91% of the solutions derived from HCD practices pursue an improved patient experience by respecting patient values. The HCD practices reviewed to date have a high level of fidelity with ISO 9241-210 principles (78%). The most commonly employed design strategy is the involvement of patients and stakeholders in the design process. Articles that have focused on patient involvement have best met the design principles. Conclusion: This study provides a holistic understanding of how HCD has been applied to date to address the patient experience. This research organises current knowledge, identifies the dimensions of patient experience that benefit most from the application of HCD and identifies the most commonly employed design activities. 
 Patient or Public Contribution: There has been no contribution in this research other than by the authors.

Large eddy simulation-bivariate sectional model for non-spherical TiO2 nanoparticle synthesis in flame

Numerical simulation of nanoparticle synthesis in flame necessitates a high level of fidelity for turbulent flame, as well as delivers more detail insight into the evolution of structure and size distribution of particles undergoing various dynamic events. In the present work, a new simulation approach coupling large-eddy simulation (LES) and bivariate sectional model is proposed to explore the synthesis of nanoparticles in turbulent flame. More specifically, the nonlinear LES-partially stirred reactor model (NLES-PaSR) is utilized to simulate the turbulent flame, wherein the gradient-type structural subgrid-scale (SGS) model for turbulent flow and partially stirred reactor (PaSR) model for turbulent combustion are considered. In addition, the volume-based bivariate sectional model is thoroughly coupled with the LES model for the first time to describe the spatiotemporally resolved formation and growth of nanoparticles using particle number concentration and surface area concentration as two sets of variables. Compared directly with the LES-monodisperse (LES-Mono) and LES-univariate sectional (LES-UniSe) models, the LES-bivariate sectional (LES-BiSe) model has significant advantages in its ability to analyze and predict the size, morphology, as well as polydispersed particle size distribution (PSD) evolution of nanoparticles at a moderate computational cost simultaneously. In the LES framework, the proposed model successfully simulates the spatiotemporally resolved formation and growth of non-spherical TiO2 nanoparticle in turbulent diffusion burner. The predicted results derived from the LES-BiSe model are in good agreement with the experimental measurements. Also, the aggregate and primary particle size distribution shows highly temporal fluctuation, which mainly results from the continuous variations of particle path induced by the unsteady turbulent combustion. Furthermore, the present work demonstrates the profound dependence of particle morphological on both spatiotemporal evolution and their intrinsic size distribution. In particular, the large aggregates in the higher sections are commonly found as irregular structure with fractal-like dimensions.

An ethnobotanical study of aromatic medicinal plants of Swat Valley, Pakistan

Abstract. Ali S, Munazir M, Sher H, Qureshi R, Akram M. 2023. An ethnobotanical study of aromatic medicinal plants of Swat Valley, Pakistan. Asian J Ethnobiol 6: 163-172. Plants play a key role in local medicines and are a source of income, as in the case of Swat, Pakistan, which is rich in medicinal plants coupled with rich in the practice of traditional knowledge and diverse cultures. So, the present study aimed to record the cultural uses of aromatic medicinal plants commonly used as medicines by herbalists from remote areas in Swat Valley. The survey was carried out in 2021 and 2023 using a semi-structured questionnaire with a total of 106 people were interviewed. A total of 33 aromatic medicinal plant species belonging to 30 genera and 14 families were identified and recorded. Lamiaceae was the most commonly used plant family (33%), followed by Apiaceae (18%), Rutaceae (9%), Zingiberaceae (6%), and Myrtaceae (6%). Herbs were commonly used for herbal recipes with 23 species (69%), followed by shrubs (12%), trees (15%), and climbers (3%). Shoots and leaves (30%) were the most commonly used parts of the plants, followed by fruits (18%), seeds (15%), bulbs (6%), rhizomes, bark, and needles (3%). In terms of mode of application, powder was the most common mode (42%), followed by dried (30%), infusions (27%), decoctions (24%), crushing (6%), and heating (3%). The highest Relative Frequency of Citation (RFC) was Skimmia laureola with 0.84, while Anisomeles indica had the lowest RFC (0.13). In terms of Fidelity Level (FL), Zingiber officinale had the highest FL (83%), while Sium latifolium had the lowest FL (13%). The recorded aromatic medicinal plants are mostly used to treat stomach problems. It has also been observed that elderly people are more knowledgeable than younger ones. Therefore, the present study suggests that these plants should be screened for new compounds linked to their reported use in the development of new therapeutics.

Medicinal plants used for management of diabetes and hypertension in Ghana

Diabetes and hypertension have been declared as a global health menace of the 21st Century. Thus, the search for potential therapeutic agents from medicinal plants for the management of diabetes and hypertension is important. This study was undertaken to investigate medicinal plants being used in the management of diabetes and hypertension by herbalists in Ghana. Data were obtained from 36 herbalists through questionnaire interviews and conversations. Botanical specimens were collected, processed and identified following standard ethnobotanical methods. Data were analyzed using Fidelity Level (FL) and Informant Consensus Factor (ICF). A total of 39 species of plants belonging to 31 families were reported being used for management of diabetes and hypertension. Eighteen of these plants are used for the treatment of hypertension, 12 species for diabetes, and 9 species for management of both diseases. Informant consensus factor was highest for plants used to treat both diseases (IFC = 0.82) followed by hypertension (ICF = 0.31) and then diabetes (IFC = 0.24). FL values were high for Carica papaya L. Moringa oleifera Lam. and Khaya senegalensis A. Juss. for the management of both diabetes and hypertension. Of the 14 species used for hypertension, Tetrapleura tetraptera (Schum. ex. Thonn.) recorded the highest FL value whiles Momordica charantia L. recorded the highest FL value for antidiabetic plants. Baphia nitida G. Lodd, Luffa aegyptiaca Mill. and Tapinanthus banguwensis (Engl. & k. Krause) Dancing are being mentioned for the first time in the management of hypertension. Herbal medicines for treatments of both diabetes and hypertension were usually prepared from multiple plant prescriptions by boiling the plant parts, and the decoctions drunk for treatments. The results show that there is substantial preclinical evidence to support the usefulness of some of these herbs as an important choice for patients with diabetes and hypertension. However, clinical studies are important to confirm the efficacy and safety of the herbal medicines prescribed by herbalists.

Process evaluation of the Invictus Pathways Program.

UniSA's Invictus Pathways Program (IPP), a service program, was originally developed to assist veterans to train for and participate in the Invictus Games. More recently, the scope of the IPP has widened to support and improve wellbeing and facilitate post traumatic growth and recovery among participants who are living with physical and mental health injuries and conditions. This paper describes the components of the IPP and reports its process evaluation. Underpinned by a pragmatic approach, data related to participant and student involvement in the IPP, the number of participant training sessions, session attendance, program activities and events, and program fidelity were compiled from process documentation that had been collected between 2017 and 2020, inclusive. Following ethics approval, semi-structured interviews were conducted with participants of the IPP, members of their family support network, and university staff to understand the operations of the IPP and satisfaction with the program. There was high fidelity for the student-led exercise training aspects of IPP; however, data collection relevant to participants' psychological outcomes, and non-training IPP events and activities did not always occur as intended. Between 2017 and 2020, 53 veterans had participated in or were still participating in the IPP, and 63 allied health students had completed placements as student trainers. Fifty-three individual training sessions were delivered in 2017, increasing to 1,024 in 2020. Seventy-one interviews were completed with key IPP stakeholders. The qualitative analysis identified four higher order themes: Implementation and fidelity of the IPP, Satisfaction with the IPP, Areas of the IPP requiring improvement and suggestions for change, and Sustainability of the IPP. Satisfaction was generally high for the IPP, although there were factors that negatively impacted the experience for some participants and their family support network. Suggestions for improvement to program components and delivery aspects were made during the interviews, and the precariousness of IPP funding and sustainability was raised as an ongoing concern. This process evaluation has demonstrated that the physical activity training components of the IPP were delivered with high levels of fidelity, and that satisfaction with the IPP is mostly high, although there are areas that could be improved. There is a need for a more structured approach to the ongoing evaluation of the IPP. This includes ensuring that program staff have a shared understanding of the purpose of evaluation activities and that these activities occur as intended. Beyond this is the need to secure funding to support the sustainability of the IPP, so that it can continue to contribute to the wellbeing of veterans living with physical and mental health conditions, and their families.

Ethnopharmacology of medicinal plants used by the Tenggerese community in Bromo Tengger Semeru National Park, Indonesia

Abstract. Bhagawan WS, Ekasari W, Agil M. 2023. Ethnopharmacology of medicinal plants used by the Tenggerese community in Bromo Tengger Semeru National Park, Indonesia. Biodiversitas 24: 5464-5477. Bromo Tengger Semeru National Park is a repository of biodiversity with profound cultural significance. The Tenggerese community residing within the park possesses rich ethnopharmacological knowledge, using medicinal plants for various ailments. This study aims to comprehensively explore the medicinal plant diversity, traditional uses, and potential pharmacological activities among the Tenggerese people. Ethnopharmacological data was gathered through interviews with informants in seven Tenggerese villages. Ethnopharmacological indices like Species Use Value (SUV) and Fidelity Level (FL) were calculated to identify the most important medicinal plants. Phytochemical analysis of selected medicinal plants was conducted using UPLC-QTOF-MS/MS, and its pharmacological potential was assessed through antibacterial tests. A total of 124 medicinal plant species from 54 families were documented. The Tenggerese community utilizes plants to treat diverse diseases, with reproductive healthcare being prominently featured. Elaeocarpus longifolius emerged as a key species with high SUV and FL values. Phytochemical analysis identified 25 compounds, including major and minor compounds, already known for their pharmacological activities elsewhere. Elaeocarpus longifolius extract showed inhibitor activity against both gram-positive and gram-negative bacteria. The findings contribute to bridging traditional knowledge and modern scientific research, offering potential avenues for the development of drug candidates from indigenous medicinal plants.

An analysis of instructor‐ and tablet‐presented conditional discriminations: Fidelity and rapidity

AbstractMatching‐to‐sample arrangements are commonly used to teach conditional discriminations. In these arrangements, instructors must systematically arrange instruction to ensure that a learner's response comes under the intended sources of stimulus control. Given the multitude of instructional considerations, the instructors' procedural fidelity has been a significant concern. Recently, LeBlanc et al. found that brief training and access to enhanced data sheets produced high levels of fidelity with experienced service providers. The current study extended LeBlanc et al. by examining the effects of a similar training on the fidelity and instructional pacing by participants with and without previous experience. The participants' performance was also compared when using a flashcard or binder (i.e., printed) arrays and relative to a tablet‐delivered instructional program. High levels of fidelity were observed following training, although pacing was slow. Slight differences in performance were observed across comparison arrays; nevertheless, the tablet‐based program outperformed instructors.

Structural properties of optimal fidelity selection policies for human-in-the-loop queues

We study optimal fidelity selection for a human operator servicing a queue of homogeneous tasks. The agent can service a task with a normal or high fidelity level, where fidelity refers to the degree of exactness and precision while servicing the task. Therefore, high-fidelity servicing results in higher-quality service but leads to larger service times and increased operator tiredness. We treat the human cognitive state as a lumped parameter that captures psychological factors such as workload and fatigue. The operator’s service time distribution depends on her cognitive dynamics and the fidelity level selected for servicing the task. Her cognitive dynamics evolve as a Markov chain in which the cognitive state increases with high probability whenever she is busy and decreases while resting. The tasks arrive according to a Poisson process and the operator is penalized at a fixed rate for each task waiting in the queue. We address the trade-off between high-quality service of the task and consequent penalty due to a subsequent increase in queue length using a discrete-time Semi-Markov Decision Process framework. We numerically determine an optimal policy and the corresponding optimal value function. Finally, we establish the structural properties of an optimal fidelity policy and provide conditions under which the optimal policy is a threshold-based policy.

Cas-Clover Editing Efficiency and Off-Target Activity in Human Hepatocytes at the KLKB1 Locus

Cutting-edge gene editing holds enormous promise for tackling devastating genetic diseases like hereditary angioedema (HAE). Here, we describe the efficient inactivation of the gene encoding pre-kallikrein, KLKB1, using our proprietary Cas-CLOVER™ high-fidelity nuclease with our non-viral, lipid nanoparticle (LNP) delivery system. Genetic inactivation of KLKB1 is an alternative clinical approach that provides durable relief to both Type I and II HAE. HAE is a rare genetic disease characterized by subcutaneous and submucosal edema, with swelling of the upper respiratory tract posing a life-threatening situation. Type I and II HAE are the most common types and are caused by mutations in the SERPING1 gene, which leads to compromised production or function of the C1 protease inhibitor. Strategies for treatment and prophylaxis include the restoration of C1 inhibitor function, or downstream antagonism of active plasma kallikrein. Safe and effective gene editing of KLKB1 could be a viable alternative for patients not adequately responding to the current standard of care. However, gene editing approaches must demonstrate an exquisitely high level of fidelity for optimal safety. To demonstrate such an approach with Cas-CLOVER, multiple guide RNAs (gRNA) targeting the human KLKB1 gene were screened in human hepatoma cell lines to identify gRNA pairs with optimal editing. Next, we evaluated KLKB1 protein reduction in primary human hepatocytes (PHH) that were incubated with LNPs encapsulating Cas-CLOVER mRNA along with each gRNA pair. Lead candidate gRNAs showed robust KLKB1 editing in a dose-responsive manner, achieving >65% editing and >85% reduction in KLKB1 protein secreted into culture medium at 0.5 ug/mL (EC90). To evaluate Cas-CLOVER off-target activity, oligo incorporation by iGUIDE was carried out by a licensed contract research organization. In this assay, double-stranded oligodeoxyribonucleotides (dsODNs) were co-electroporated with Cas-CLOVER mRNA, along with our lead KLKB1 gRNA pair, in the Huh7 cell line, and candidate off-target sites were identified by Illumina next-generation sequencing. Off-target activity was assessed by amplicon-seq at the eight top sites nominated by iGUIDE. In PHHs treated with 0.5 ug/mL of Cas-CLOVER LNPs, off-target editing was detected in 3/8 sites at very low levels (<0.25%). Remarkably, this low level of off-target editing remained unchanged when PHHs were treated with 10-fold higher concentrations of Cas-CLOVER LNP. For further evaluation of our platform, we sought to determine KLKB1 editing efficiency and fidelity in a mouse model of liver humanization. TK-Nog mice engrafted with PHHs were treated with a single intravenous injection of an LNP formulation co-encapsulating Cas-CLOVER mRNA and our lead KLKB1 gRNA pair. Amplicon-seq analysis demonstrated that 60% of KLKB1 alleles in the liver were edited. Importantly, no off-target editing was detected among the top eight sites identified by iGUIDE, including the three off-target sites validated in cultured PHHs. Next, we evaluated efficacy and tissue specificity of our platform in wild type mice. C57BL/6 male and female mice were dosed with LNP encapsulating Cas-CLOVER mRNA and mouse Klkb1-targeting gRNAs. A single intravenous LNP injection achieved high Klkb1 editing (>50% of haploid genomes) in the liver and >80% reduction in serum pre-kallikrein levels. No Klkb1 editing was detected in gonads. In summary, these results highlight the efficacy and specificity of our high-fidelity Cas-CLOVER gene editing platform that enables targeted and therapeutically relevant kallikrein reduction in a fully non-viral manner. These data provide a promising foundation for the development of a highly specific gene editing therapy for HAE.

High-fidelity post-impact residual strength assessment for composite aircraft sustainment

AbstractHigh-fidelity simulation tools have significant potential to support composite aircraft sustainment, though further study is required on incorporating the complex impact damage field. In this paper, compressive residual strength assessment is investigated using the high-fidelity computational tool BSAMTM. The experimental impact damage was mapped and modelled at a high-fidelity level, which included ply-by-ply definition of the geometry of the impact indentation, fibre fracture in the plies and delamination in ply interfaces. It was shown that applying a small lateral displacement or ‘pseudo-impact’ step was highly effective in generating matrix cracks in the impact region, which provided a suitably realistic representation of the interconnected damage map through-the-thickness. It was found that inclusion of all damage modes in the post-image damage map at a high-fidelity definition was essential due to the strong degree of interaction between damage modes. The results support improved sustainment of defence platforms, through enhanced predictive capability and understanding.

Botanical Assessment of Remnant Floodplain Habitats Along Plaster Creek, Kent County, Michigan: Assessing Changes Since the 1890s

Plaster Creek, a tributary of the Grand River, drains a 58-square mile watershed in Kent County, Michigan. Its headwaters originate in the agriculturally dominated southwestern portion of the county, and then it meanders through residential, commercial, and urban areas of Kentwood and Grand Rapids before it empties into the Grand River about one mile south of downtown Grand Rapids. Much of Plaster Creek’s original floodplain, like the rest of its watershed, has been drastically altered and degraded over time due to the development of residential neighborhoods, commercial properties, agriculture, and industrial zones. Floodplains house unique assemblages of Michigan’s native biodiversity and sustain stream and watershed health, warranting their preservation and restoration. Over several seasons, Calvin University Herbarium’s Emma Cole Grand Rapids Flora Project inventoried seven remnant floodplain sites along Plaster Creek from near its source in Gaines Township to downstream areas within the City of Grand Rapids. A total of 438 species of vascular plants were documented for the combined seven floodplains, of which 341 (77.9%) are native. Floristic Quality Assessments were calculated for each of the sites with values ranging from a Total Floristic Quality Index (FQI) of 30.4 (Total Mean C = 2.8) at the smallest site with only 93 native species (78.7%), to a Total FQI of 52.7 (Total Mean C = 3.6) at the Ken-O-Sha Park floodplain, with 176 (82.2%) native species. The Stanaback Park floodplain had a similar number of native species, 174 (80.9%), and a Total FQI of 48.4 (Total Mean C = 3.3). In order to make comparisons with floristic information compiled by Emma Cole in the 1890s, a Floristic Quality Assessment was calculated based on 65 species (98.5% native) collected and reported by Cole (1901) as occurring along Plaster Creek. The Total FQI of Cole’s list was 48.4 (Total Mean C = 6.0), and 27 of her species have a high level of fidelity to a narrow range of ecological conditions, five of which today hold Special Concern, Threatened, or Endangered status in Michigan. By comparing our inventory findings with the floristic information gathered by Cole, this study highlights changes that have taken place in the Plaster Creek floodplain over the past 120+ years, describes the present-day condition of Plaster Creek’s floodplains, and can be used to inform future ecological restoration efforts along this and other local creeks.

The Challenges and Opportunities of Implementing Montessori Education in the Public Sector

Over the past two decades, Montessori education in the United States has rapidly expanded from the private to the public sector. This expansion has created important questions about whether or not the Montessori approach can thrive alongside the education standards and accountability movement in the public sector. Questions also exist as to precisely who is benefitting from this expansion of and investment in public Montessori. To examine these topics, this study focused on South Carolina, the state with the highest number of public programs in the United States. We used implementation surveys, classroom observations, and teacher interview data collected by the research team and student record data collected by the state of South Carolina to conduct the analysis, which consisted of three parts. First, we investigated to what extent public programs in South Carolina are able to implement Montessori education with fidelity to the model. Second, we considered what program characteristics were related to higher levels of Montessori implementation fidelity. Third, we analyzed which children had access to higher-fidelity Montessori programs. Generally, findings indicated that, despite challenges created by the education standards and accountability movement and concerns expressed by educators about authenticity, most programs in South Carolina were implementing Montessori with fidelity. Several characteristics were associated with higher levels of fidelity, including the age of the program. Findings also indicated that Black, Hispanic, and students from low-income families were disproportionately participating in lower fidelity programs. Our study provides an in-depth analysis of the challenges and opportunities associated with government trying to implement successful private-sector education models in the public sector.

3D Printing of Bioactive Gel-like Double Emulsion into a Biocompatible Hierarchical Macroporous Self-Lubricating Scaffold for 3D Cell Culture.

The interconnected hierarchically porous structures are of key importance for potential applications as substrates for drug delivery, cell culture, and bioscaffolds, ensuring cell adhesion and sufficient diffusion of metabolites and nutrients. Here, encapsulation of a vitamin C-loaded gel-like double emulsion using a hydrophobic emulsifier and soy particles was performed to develop a bioactive bioink for 3D printing of highly porous scaffolds with enhanced cell biocompatibility. The produced double emulsions suggested a mechanical strength with the range of elastic moduli of soft tissues possessing a thixotropic feature and recoverable matrix. The outstanding flow behavior and viscoelasticity broaden the potential of gel-like double emulsion to engineer 3D scaffolds, in which 3D constructs showed a high level of porosity and excellent shape fidelity with antiwearing and self-lubricating properties. Investigation of cell viability and proliferation using fibroblasts (NIH-3T3) within vitamin C-loaded gel-like bioinks revealed that printed 3D scaffolds offered brilliant biocompatibility and cell adhesion. Compared to scaffolds without encapsulated vitamin C, 3D scaffolds containing vitamin C showed higher cell viability after 1 week of cell proliferation. This work represented a systematic investigation of hierarchical self-assembly in double emulsions and offered insights into mechanisms that control microstructure within supramolecular structures, which could be instructive for the design of advanced functional tissues.

Mangroves species diversity and their use as medicinal plants by coastal communities of Pesawaran Lampung

As a means of adaptation to extreme habitats, mangrove plants produce a variety of secondary metabolic substances that have the potential as herbal medicines. Empirically, coastal communities have utilized and proven the medicinal properties of mangrove plants. The study’s objective was to determine the species diversity of mangrove plants and their use as herbal medicine in the Coastal of Pesawaran, Lampung Province. Mangrove plant diversity was obtained through vegetation analysis and the diversity index calculation. The use of mangroves as medicine was obtained through in-depth interviews followed by descriptive analysis and calculation of species use value, plant part value, and fidelity level. The results showed that the mangrove forest on the Pesawaran Coast consisted of 21 mangrove species from 12 families, with a moderate diversity index (H’= 1.9312). Most of the users of mangrove plants as medicine ethnically were Bugis (55%), aged over 40 years (62.5%), have an income ranging from 100--167 USD / month (54%), and have secondary education (37.5%). The community uses Avicenia alba as an antiseptic, Acanthus ilicifolius as an antiseptic, Rhizophora apiculata as an antiseptic and analgesic, and R. stylosa as an antiseptic and anti-rheumatism. The Species use value of R. apiculate was the highest (0.25), while the other three species only reached 0.125. Leaves and bark were parts of the plant used as medicine, where leaves have the highest plant part value of 91.67%, and the remaining 8.33% was bark. The highest fidelity level was obtained by R. apiculate as an antiseptic (88%), followed successively by R. stylosa as an antiseptic (50%), R. stylosa as an analgesic, and A. alba as an antiseptic (13%).

Integrating Immersive Scenes and Interactive Contextual Clue Scaffolding Into Decision-Making Analysis Ability Training Game

In the post-pandemic era, distance education and training modes without space constraints are gradually becoming a new trend in training. Game-based training combined with situational simulations is a potential approach. This study used a simulation company scenario and a decision-making analysis task of promotion assessment as the game context, and proposed an innovative game mechanism integrating the simulation situation scenario and the interactive contextual clue scaffolding as a guide to enhance learning effectiveness. This study used the Gather Town platform to design a simulation office scenario, and Google Forms to design interactive contextual clue scaffolding. This study conducted empirical research to evaluate this mechanism, with a total of 60 participants divided into an experimental group that conducted game activities and a control group that was only provided with document files as scaffolding for comparative analysis. The results of the study revealed that the proposed exploration guidance mechanism of integrating immersive scenes and interactive contextual clue scaffolding could allow learners to experience a high level of game fidelity and increase the discovery rate and usefulness of the scaffolding. It also reduced learning anxiety, maintained learning motivation and high flow, and resulted in significantly higher learning effectiveness than the control group approach.

Exploration of the Wild Edible Plants Used for Basic Health Care by Local People of Bahawalpur and Adjacent Regions, Pakistan.

The current study aimed to explore the traditional knowledge and practices of indigenous communities in Bahawalpur and adjacent regions, Pakistan, to treat a variety of diseases with a particular focus on the usage of ethnomedicinal wild plants. The objective of the study was to collect and evaluate local and indigenous knowledge regarding the quantity, variety, use and management of resources by the people. Data were gathered using Rapid Appraisal Approach (RAA), which involved in-person interviews. The data were analyzed using descriptive statistics methods as well as common ethnobotanical analytical techniques viz. use value (UV), relative frequency of citation (RFC), informant consensus factor (ICF), fidelity level (FL), relative importance (RI), frequency index (FI), family use value (FUV), family importance value (FIV), popular therapeutic use value (POPUT), plant part value (PPV), preference ranking (PR), cultural significance index (CSI), rank order priority (ROP) and Jaccard index (JI). A total of 158 plant species from 49 families were identified. The perennial herbs made up 72% of the documented species of therapeutic plants. There were 21 different species in each of the two major plant families i.e., Fabaceae and Poaceae. The maximum ICF were calculated for gonorrhea and paralysis (1.0) and maximum POPUT was calculated for skin disorders (0.088) and cough (0.077), respectively. The maximum UV was found for Rumex crispus (0.57) and RI for Leucaena leucocephala (4.38). Heliotropium crispum had the highest FL value (83%) and is used to treat kidney diseases. Alhagi maurorum had the greatest RFC (0.009) and FI (0.93). Arecaceae (0.45) and Fabaceae (13.39%) had the greatest FUV while Poaceae and Fabaceae had the highest FIV (13.29 for each). Leaves were the most frequently used plant part (35%). Alhagi maurorum (4.0) and Rumex crispus (32.57) had the highest CSI and ROP, respectively. The maximum JI (28.31) was calculated for Cholistan desert. These results showed the local population's reliance on herbal remedies to treat prevalent illnesses. To demonstrate the viability of species, it's crucial to emphasize both the use and conservation of these species. Adopting innovative applications, enhancing their value, and prioritizing the protection of multipurpose wild plants in inhabited environments is vital.