Background: Workplace-based fitness program has been introduced as a means of motivating employees towards participating in active living, maintenance of healthy lifestyle behaviors and increase brain power and productivity. However, the effects of such program on development of individualized fitness program have not been evaluated. Objective: The focus of this study was to investigate the impact of workplace-based fitness on development of an individualized physical activity program among health workers. Method: The cross-sectional study recruited 177 clinical and administrative workers in a tertiary health institution. Data on demographic characteristics, health-benefit of exercise, physical activity level and development of an individualized physical activity program were obtained using a self-administered questionnaire. Data were summarized using a descriptive statistic of percentage and frequency distribution. Results: 172 (97.1%) of the participants are aware of the health benefits of physical activity and the monthly physical fitness program held in the hospital. Only 71 (40.1%) of those that are aware actually participated in the fitness program. 52 (73.2%) of those that participated in the monthly program have developed a personalized fitness program as a result of the motivation received through the monthly fitness program. Conclusions: Hospital workers are aware of the health-related benefits of physical activity however higher level of awareness does not always translate to high level of participation in physical activity. A well-designed workplace-based physical activity can help staff in developing an individualised fitness program. Employers of labour should incorporate workplace-based physical activity into their organisation as a means of promoting the health and fitness of their employee thereby improving productivity and reducing health cost of preventive diseases.