High Levels Of Abundance Research Articles

Stock assessment, spatial distribution and biological parameters of the clam Venerupis decussata along the Sfax coasts (Tunisia, Central Mediterranean)

The grooved carpet shell Venerupis decussata (=Ruditapes decussatus) (Linnaeus, 1758) is a Mediterranean autochthonous species that in Tunisia is heavily harvested from natural populations. It represents one of the important economic issues, especially in terms of employment and the trade balances of the country via its exportation. Despite its economic value, no studies have been carried out about the spread of this mollusc. Thus the status of this species is still poorly known and there is a knowledge gap about its stocks assessment. This study aimed to investigate the distribution, abundance and population structure of the carpet shell clam in Sfax coastal areas. In terms of geographic occupation, maps of the population distribution were drawn. The results showed a scattered distribution pattern of the species according to location ranging between 0 and 152 ind m−2. The consequence was a remarkable biomass which represented 891 tonnes of total fresh weight and high abundance levels reaching over 261.7 ± 84.2 million individuals estimated in the area of 4182 hectares. The distribution of the species has also been investigated in consideration with the size which varied markedly according to location, the shell length ranged between 2 and 41 mm. The overall sex ratio (F:M) was 1:1.17, which was significantly different from parity (1:1). The size at which 50% of the population reached maturity was 24.08 and 24.76 mm shell length for males and females, respectively. Findings of the current study suggest that V. decussata proliferates along southern Tunisian coasts.

Coexisting invasive gobies reveal no evidence for temporal and trophic niche differentiation in the sublittoral habitat of Lake Erhai, China

AbstractNiche differentiation facilitates the coexistence of species within a community through avoidance of competition via mechanisms involving spatial, temporal and/or trophic dimensions. Where invasive species coexist in their non‐native range, niche differentiation could allow their persistence at higher levels of abundance. Here, we tested whether there was temporal and/or trophic niche differentiation between two congeneric invasive goby species (Rhinogobius cliffordpopei and Rhinogobius giurinus) in the sublittoral habitat of Lake Erhai (south‐west China) through quantifying their diel and seasonal rhythm of locomotion activities, feeding activities and diet composition. Both species displayed two similar diel peaks in activity and two main feeding periods (6:00–10:00 and 18:00–22:00), with rhythms of locomotion and feeding activity not differing significantly between the species in each season. Their diets had a high degree of overlap, being primarily composed of macrozooplankton, aquatic insects and shrimp larvae, with no diel changes across the seasons. Thus, in this habitat, there was no clear temporal or trophic niche differentiation between the invasive congeners, indicating their coexistence with high temporal and trophic overlap. In conjunction with data from the littoral and profundal habitats, the gobies revealed different strategies across the habitats (e.g. spatial segregation, trophic niche differentiation) that minimised their competitive interactions and promoted their coexistence. This suggests that the interactions of invasive fishes during the integration into native communities can be context dependent, varying according to factors including habitat and the availability of food resources.

THE RISE AND ROLE OF CIVIL SOCIETY IN PAKISTAN: COMPARATIVE ANALYSIS OF DEFINITIONS, TENDENCIES, AND PERSPECTIVES

What does civil society mean in an unevenly developed country with a high level of poverty and abundance of traditional and quasi-traditional norms and structures? What is the history of the evolving Pakistani civil society, what trends and perspectives awaits it in the conditions of long lasting deep crises in economy and security sphere? How does the civil society react to the strengthening of ideology of Islamic radicalism and Islamic nationalism? These are some issues discussed in the article. It does not claim to cover in full the relatively new phenomenon in a specific Asian country occupying a remarkable place in the policy of Russia to the south of its borders.

16S rDNA analysis of archaea indicates dominance of Methanobacterium and high abundance of Methanomassiliicoccaceae in rumen of Nili-Ravi buffalo

The molecular diversity of rumen methanogens was investigated using 16S rDNA gene library prepared from the rumen contents of Nili-Ravi buffaloes. Microbial genomic DNA was isolated from four adult male fistulated buffaloes and PCR conditions were set up using specific primers. Amplified product was cloned into a suitable vector, and the inserts of positive clones were sequenced. A total of 142 clones were examined, and the analysis revealed 46 species level (0.01 distance) operational taxonomic units (OTUs). Twenty six OTUs comprising 89 clones (63% of the total clones) were taxonomically assigned to Methanobacterium genus and the majority of them had highest percent identity with Methanobacterium flexile among cultured methanogens. Five OTUs comprising 27 clones (19% of total clones) were taxonomically assigned to Methanomicrobium genus and these clones showed highest sequence identity with Methanomicrobium mobile. Only two OTUs comprising 6 clones (4% of total clones) were assigned to Methanobrevibacter genus. A total of 17 clones belonging to 10 species level OTUs showed highest percent identity (ranging from 85 to 95%) with Methanomassilicoccus luminyensis and were taxonomically classified as Methanomassiliicocaceae. Out of the 142 rDNA clones, 112 clones, which constitute 79% of the total clones representing 42 OTUs, had less than 98.5% sequence identity with any of the cultured strains of methanogens and represent novel species of methanogens. This study has revealed the largest assortment of hydrogenotrophic methanogen phylotypes ever identified from the rumen of Nili-Ravi buffaloes. The study indicates that Methanobacterium is the most dominant methanogen in the rumen of Nili-Ravi buffalo. This is also the first report on the presence of methanogens phylogenetically close to M. luminyensis, an H2 dependent methylotrophic methanogen, in the rumen of buffaloes at such a high level of abundance.

A new endothiodont dicynodont (Therapsida, Anomodontia) from the Permian Ruhuhu Formation (Songea Group) of Tanzania and its feeding system

ABSTRACTCranial material of the dicynodont Endothiodon from the middle fossiliferous horizon of the Permian Ruhuhu Formation (Ruhuhu Basin, Tanzania) is described as a new species, E. tolani. Endothiodon tolani is distinguished from other Endothiodon species by the absence of a pineal boss and the presence of tusks in most specimens. Although some parts of the Ruhuhu Formation probably correlate with the Eodicynodon or Tapinocephalus assemblage zones of the South African Karoo Basin, it is uncertain whether E. tolani is older than E. bathystoma or E. mahalanobisi. There is less evidence for extensive anteroposterior translation of the mandible in E. tolani than in other dicynodonts. The jaw joint is specialized for allowing some medial-lateral motion. Mastication involved unilateral chewing. Flexure of the postdentary bones relative to the fused dentaries permitted the posterolaterally directed rows of teeth on one side of the skull and mandible to move past one another. The long tooth rows and prominent horn-covered ridges and grooves on the palate and mandible of Endothiodon are unique among dicynodonts. Comparisons with other taxa suggest that its feeding system may have been specialized for cutting three-dimensional objects such as stems or rhizomes. The dicynodont feeding system is very stereotyped throughout the clade's history, but Endothiodon escaped the constraints that affected other dicynodonts, allowing it to evolve a unique skull morphology. This evolutionary flexibility did not translate into high taxonomic richness (we recognize only three species of Endothiodon), but Endothiodon was able to achieve high levels of relative abundance in some basins.http://zoobank.org/urn:lsid:zoobank.org:pub:9686F708-2E6E-40EB-956F-05659889684FSUPPLEMENTAL DATA—Supplemental materials are available for this article for free at www.tandfonline.com/UJVP

Response to Willette et al. (2015)

We agree with the authors (WSV) that the environment in Cook Inlet changes in complicated ways. However, WSV’s listing of several unresolved questions (chiefly, in our view, whether the results apply to smaller fish or to very shallow inshore environments and whether the bottom depth of fish nets can be predicted with sufficient accuracy) is insufficient rationale for discounting the large potential benefits to both commercial and sport fishery sectors of using shallower nets. Our paper included an example that illustrated the likely relative harvest rates of using nets fishing at two specific depths (3.5 and 5.5 m). As we specifically noted in the caption to figure nine, we assumed net depths ‘…to be directly proportional to the number of meshes; if the effective maximum depth of the net is different (because mesh size varies or nets billow under strong tidal currents), this would amount to a lateral shift of the curves along the x-axis’. The analysis remains valid if we were to replace an assumed fixed-depth net with a variable net depth that oscillates over the tidal cycle; in this case, we would integrate the relative harvest rates of the nets as their effective depth changes over time. In practical terms, we would substitute the average effective depth of the net during that part of the tidal cycle chosen for a fishery opening and read off the relative harvest rates from the existing figure nine. It is important to remember that the current management regime in Cook Inlet is neither the result of a carefully tailored biological analysis nor one based on a careful economic analysis of what management system will provide the best economic value to the state. Rather, the current system is a set of inherited rules now nearly 50 years old that managed harvest levels adequately in an earlier time when severe conservation concerns for Chinook did not exist and which more or less satisfied the competing interests of the stakeholders. As a result, all fishing sectors could obtain satisfactory livelihoods and co-exist. Because the regulations allowed ‘co-existence,’ no one really questioned whether they were economically and biologically optimal, and there was thus little pressure to change. In our view, the advent of new information derived from telemetry technologies should allow managers to fill in key knowledge gaps and re-assess management regulations in this light. As WSV note, fisheries management in Cook Inlet has become ‘highly contentious;’ we agree, but do not agree that maintaining status quo management in the face of recent major environmental change is the best option, because major social, economic, and (possibly) biological loss is occurring. The key questions are as follows: (a) Is the existing management structure close to the best possible? and (b) If not, what steps could be taken to improve the management? Our results suggest that improvements to the management structure are indeed possible, because technological developments in acoustic telemetry provide an unprecedented ability to measure the movements of fish. The ability of properly implemented telemetry systems to identify radical alternatives to the status quo is widely underappreciated in our view, not just in Alaska but worldwide. In broad terms, our past inability to know where and how fish move has blinded us to opportunities to improve our management. In the current case, telemetry data allows us to identify a possible new regulatory solution that should improve the economic utility of both fishing sectors, a previously unrecognized possibility. Major restrictions have been imposed on the Eastside Setnet (ESSN) Fishery relative to earlier periods. These resulted in huge declines in fishing effort and revenue, which also occur at a time when the target species (sockeye) is at high levels of abundance, exacerbating

Relation between biological parameters of Pacific salmons of the genus Oncorhynchus and their population dynamics off the northeastern Kamchatka Peninsula

Results are provided of a 7-year study of biological parameters in females of three Pacific salmons of the genus Oncorhynchus (pink salmon O. gorbuscha, chum salmon O. keta, and sockeye salmon O. nerka) in the Olyutorsky and Karaginsky gulfs, Bering Sea. Abundance of the pink salmon is identified as the main determining factor of the interannual dynamics of maturity index in female Pacific salmon in coastal waters. Maturity index rises at high levels of abundance as a result of differently directed changes in two parameters: decreasing body weight and increasing ovary weight. In female chum salmon, maturity index depends on the age structure of the population and body weight dynamics of different age groups, factors influenced by high abundance of some pink salmon generations, and does not depend on the abundance of spawning chum salmon. The revealed association between pink salmon and sockeye salmon in dynamics of their biological parameters may result from the similarity of their diets; during the last year of fattening in the sea, the sockeye salmon is affected by the pink salmon, the most abundant of the three species. The interannual variation of biological parameters in pink salmon and chum salmon is more pronounced in Olyutorsky Gulf than in Karaginsky Gulf.

Analysis of microbial community structure and composition in leachates from a young landfill by 454 pyrosequencing.

Microorganisms are responsible for degrading the raw leachate generated in sanitary landfills, extracting the soluble fraction of the landfill waste and biotransforming organic matter and toxic residues. To increase our understanding of these highly contaminated ecosystems, we analyzed the microbial communities in the leachate produced by three landfill cells of different ages. Using high-throughput 454 pyrosequencing of the 16S rRNA gene, we describe the structure of the leachate communities and present their compositional characteristics. All three communities exhibited a high level of abundance but were undersampled, as indicated by the results of the rarefaction analysis. The distribution of the taxonomic operational units (OTUs) was highly skewed, suggesting a community structure with a few dominant members that are key for the degradation process and numerous rare microorganisms, which could act as a resilient microorganism seeder pool. Members of the phylum Firmicutes were dominant in all of the samples, accounting for up to 62% of the bacterial sequences, and their proportion increased with increasing landfill age. Other abundant phyla included Bacteroidetes, Proteobacteria, and Spirochaetes, which together with Firmicutes comprised 90% of the sequences. The data illustrate a microbial community that degrades organic matter in raw leachate in the early stages, before the methanogenic phase takes place. The genera found fit well into the classical pathways of anaerobic digestion processes.

Effect of abandonment on diversity and abundance of free-living nitrogen-fixing bacteria and total bacteria in the cropland soils of Hulun Buir, Inner Mongolia.

In Inner Mongolia, steppe grasslands face desertification or degradation because of human over activity. One of the reasons for this condition is that croplands have been abandoned after inappropriate agricultural management. The soils in these croplands present heterogeneous environments in which conditions affecting microbial growth and diversity fluctuate widely in space and time. In this study, we assessed the molecular ecology of total and free-living nitrogen-fixing bacterial communities in soils from steppe grasslands and croplands that were abandoned for different periods (1, 5, and 25 years) and compared the degree of recovery. The abandoned croplands included in the study were natural restoration areas without human activity. Denaturing gradient gel electrophoresis and quantitative PCR (qPCR) were used to analyze the nifH and 16S rRNA genes to study free-living diazotrophs and the total bacterial community, respectively. The diversities of free-living nitrogen fixers and total bacteria were significantly different between each site (P<0.001). Neither the total bacteria nor nifH gene community structure of a cropland abandoned for 25 years was significantly different from those of steppe grasslands. In contrast, results of qPCR analysis of free-living nitrogen fixers and total bacteria showed significantly high abundance levels in steppe grassland (P<0.01 and P<0.03, respectively). In this study, the microbial communities and their gene abundances were assessed in croplands that had been abandoned for different periods. An understanding of how environmental factors and changes in microbial communities affect abandoned croplands could aid in appropriate soil management to optimize the structures of soil microorganisms.

Identification and expression analysis of WRKY family genes under biotic and abiotic stresses in Brassica rapa.

WRKY proteins constitute one of the largest transcription factor families in higher plants, and they are involved in multiple biological processes such as plant development, metabolism, and responses to biotic and abiotic stresses. Genes of this family have been well documented in response to many abiotic and biotic stresses in many plant species, but not yet against Pectobacterium carotovorum subsp. carotovorum and Fusarium oxysporum f.sp. conglutinans in any of the plants. Moreover, potentiality of a specific gene may vary depending on stress conditions and genotypes. To identify stress resistance-related potential WRKY genes of Brassica rapa, we analyzed their expressions against above-mentioned pathogens and cold, salt, and drought stresses in B. rapa. Stress resistance-related functions of all Brassica rapa WRKY (BrWRKY) genes were firstly analyzed through homology study with existing biotic and abiotic stress resistance-related WRKY genes of other plant species and found a high degree of homology. We then identified all BrWRKY genes in a Br135K microarray dataset, which was created by applying low-temperature stresses to two contrasting Chinese cabbage doubled haploid (DH) lines, Chiifu and Kenshin, and selected 41 BrWRKY genes with high and differential transcript abundance levels. These selected genes were further investigated under cold, salt, and drought stresses as well as after infection with P. carotovorum subsp. carotovorum and F. oxysporum f.sp. conglutinans in B. rapa. The selected genes showed an organ-specific expression, and 22 BrWRKY genes were differentially expressed in Chiifu compared to Kenshin under cold and drought stresses. Six BrWRKY genes were more responsive in Kenshin compared to Chiffu under salt stress. In addition, eight BrWRKY genes showed differential expression after P. carotovorum subsp. carotovorum infection and five genes after F. oxysporum f.sp. conglutinans infection in B. rapa. Thus, the differentially expressed BrWRKY genes might be potential resources for molecular breeding of Brassica crops against abiotic and biotic stresses and several genes, which showed differential expressions commonly in response to several stresses, might be useful for multiple stress resistance. These findings would also be helpful in resolving the complex regulatory mechanism of WRKY genes in stress resistance and for this further functional genomics study of these potential genes in different Brassica crops is essential.

Climate change, pink salmon, and the nexus between bottom-up and top-down forcing in the subarctic Pacific Ocean and Bering Sea

Climate change in the last century was associated with spectacular growth of many wild Pacific salmon stocks in the North Pacific Ocean and Bering Sea, apparently through bottom-up forcing linking meteorology to ocean physics, water temperature, and plankton production. One species in particular, pink salmon, became so numerous by the 1990s that they began to dominate other species of salmon for prey resources and to exert top-down control in the open ocean ecosystem. Information from long-term monitoring of seabirds in the Aleutian Islands and Bering Sea reveals that the sphere of influence of pink salmon is much larger than previously known. Seabirds, pink salmon, other species of salmon, and by extension other higher-order predators, are tightly linked ecologically and must be included in international management and conservation policies for sustaining all species that compete for common, finite resource pools. These data further emphasize that the unique 2-y cycle in abundance of pink salmon drives interannual shifts between two alternate states of a complex marine ecosystem.

Biotic evolution and its relation with geological events in the Proterozoic Yanshan Basin, North China

The Yanshan Basin, located in northern North China, underwent three primary stages in the Mesoproterozoic, and corresponding biotas have been summarized for each stage in the present study. The three stages are stage A (the lower part of the Changcheng Group which dominated by clastic sediments), stage B (the upper part of the Changcheng Group to the Jixian Group which characterized by carbonate sediments), and stage C (the upper part of the Jixian Group which dominated by clastic sediments). This paper will focus on the evolution of biota during stage B. Microbiota of the stage B exhibits a high level of diversity and abundance. Planktonic microfossils have various forms and complex decorations. Most microfossils are small. In stage B, there was great variation in the biotic abundance and diversity, the average diameter of spherical benthic microfossils, and the maximum diameter of the spherical microfossils. We analyzed these parameters and identified two typical biotic events, and both biotic events were corresponded with geological events. The first event occurred in the third member of the Dahongyu Formation. In this era, the maximum diameter of the silicified microfossils significantly increased, and large, spherical planktonic specimens are abundant. The flourishing of large organisms in the strata is presumably related to volcanic activity. The second event occurred in the third member of the Gaoyuzhuang Formation. During this era, the microfossil assemblage changed significantly. The changes correspond with a decline in stromatolites as well as the transformations of the carbon isotope ratios and the elemental geochemistry.

Evolution of Chloroplast Transcript Processing in Plasmodium and Its Chromerid Algal Relatives

It is well understood that apicomplexan parasites, such as the malaria pathogen Plasmodium, are descended from free-living algae, and maintain a vestigial chloroplast that has secondarily lost all genes of photosynthetic function. Recently, two fully photosynthetic relatives of parasitic apicomplexans have been identified, the ‘chromerid’ algae Chromera velia and Vitrella brassicaformis, which retain photosynthesis genes within their chloroplasts. Elucidating the processes governing gene expression in chromerid chloroplasts might provide valuable insights into the origins of parasitism in the apicomplexans. We have characterised chloroplast transcript processing pathways in C. velia, V. brassicaformis and P. falciparum with a focus on the addition of an unusual, 3′ poly(U) tail. We demonstrate that poly(U) tails in chromerids are preferentially added to transcripts that encode proteins that are directly involved in photosynthetic electron transfer, over transcripts for proteins that are not involved in photosynthesis. To our knowledge, this represents the first chloroplast transcript processing pathway to be associated with a particular functional category of genes. In contrast, Plasmodium chloroplast transcripts are not polyuridylylated. We additionally present evidence that poly(U) tail addition in chromerids is involved in the alternative processing of polycistronic precursors covering multiple photosynthesis genes, and appears to be associated with high levels of transcript abundance. We propose that changes to the chloroplast transcript processing machinery were an important step in the loss of photosynthesis in ancestors of parasitic apicomplexans.

Promoter occupancy of the basal class I transcription factor A differs strongly between active and silent VSG expression sites in Trypanosoma brucei

Monoallelic expression within a gene family is found in pathogens exhibiting antigenic variation and in mammalian olfactory neurons. Trypanosoma brucei, a lethal parasite living in the human bloodstream, expresses variant surface glycoprotein (VSG) from 1 of 15 bloodstream expression sites (BESs) by virtue of a multifunctional RNA polymerase I. The active BES is transcribed in an extranucleolar compartment termed the expression site body (ESB), whereas silent BESs, located elsewhere within the nucleus, are repressed epigenetically. The regulatory mechanisms, however, are poorly understood. Here we show that two essential subunits of the basal class I transcription factor A (CITFA) predominantly occupied the promoter of the active BES relative to that of a silent BES, a phenotype that was maintained after switching BESs in situ. In these experiments, high promoter occupancy of CITFA was coupled to high levels of both promoter-proximal RNA abundance and RNA polymerase I occupancy. Accordingly, fluorescently tagged CITFA-7 was concentrated in the nucleolus and the ESB. Because a ChIP-seq analysis found that along the entire BES, CITFA-7 is specifically enriched only at the promoter, our data strongly indicate that monoallelic BES transcription is activated by a mechanism that functions at the level of transcription initiation.

Comparative Secretome Analysis of Epithelial and Mesenchymal Subpopulations of Head and Neck Squamous Cell Carcinoma Identifies S100A4 as a Potential Therapeutic Target

Epithelial-mesenchymal transition (EMT) is a key contributor in tumor progression and metastasis. EMT produces cellular heterogeneity within head and neck squamous cell carcinomas (HNSCC) by creating a phenotypically distinct mesenchymal subpopulation that is resistant to conventional therapies. In this study, we systematically characterized differences in the secretomes of E-cadherin high epithelial-like and E-cadherin low mesenchymal-like subpopulations using unbiased and targeted proteomics. A total 1765 proteins showed significant changes with 177 elevated in the epithelial subpopulation and 173 elevated in the mesenchymal cells. Key nodes in affected networks included NFκB, Akt, and ERK, and most implicated cellular components involved various aspects of the extracellular matrix. In particular, large changes were observed in multiple collagens with most affected collagens at much higher abundance levels in the mesenchymal subpopulation. These cells also exhibited a secretome profile resembling that of cancer-associated fibroblastic cells (CAF). S100A4, a commonly used marker for cancer-associated fibroblastic cells, was elevated more than 20-fold in the mesenchymal cells and this increase was further verified at the transcriptome level. S100A4 is a known mediator of EMT, leading to metastasis and EMT has been proposed as a potential source of cancer-associated fibroblastic cells in solid tumors. S100A4 knockdown by small interfering RNA led to decreased expression, secretion and activity of matrix metalloproteinase 2, as verified by quantitative PCR, multiple reaction monitoring and zymography analyses, and reduced invasion in collagen-embedded spheroids. Further confirmation in three-dimensional organotypic reconstructs showed less invasion and advanced differentiation in the S100A4 RNA interference samples. Orthotopic metastasis model, developed to validate the findings in vivo, demonstrated a decrease in spontaneous metastasis and augmented differentiation in the primary tumor in siS100A4 xenografts. These results demonstrate the value of secretome profiling to evaluate phenotypic conversion and identify potential novel therapeutic targets such as S100A4.

Phylogeography sheds light on the central–marginal hypothesis in a Mediterranean narrow endemic plant

Understanding the factors that shape variation in genetic diversity across the geographic ranges of species is an important challenge in the effort to conserve evolutionary processes sustaining biodiversity. The historical influences leading to a central-marginal organization of genetic diversity have been explored for species whose range is known to have expanded from refugia after glacial events. However, this question has rarely been addressed for Mediterranean endemic plants of azonal habitats such as rocky slopes or screes. In this context, this comprehensive study examined molecular and field data from Arenaria provincialis (Caryophyllaceae), a narrow endemic plant of south-eastern France. Across the whole geographic range, an investigation was made of whether high levels of abundance and genetic diversity (estimated from amplified fragment length polymorphism markers) are centrally distributed, to evaluate the relevance of the central-marginal hypothesis. Phylogeographic patterns inferred from chloroplast DNA (cpDNA) were used, applying Bayesian methods to test the influence of past biogeographic events. Multivariate analysis combining phylogeographic and ecological data was used to reveal the historical and ecological distinctiveness of populations. Despite the narrow distribution of A. provincialis, a high level of nucleotide variation is found within cpDNA loci, supporting its persistence throughout the Pleistocene period. The area characterized by the highest genetic diversity is centrally located. Structured phylogeography and Bayesian factor analysis supported the hypothesis that the central area of the distribution was the source of both westward and eastward migrations, probably during arid periods of the Pleistocene, and more recently was a crossroads of backward migrations. By contrast, the two areas located today at the range limits are younger, have reduced genetic diversity and are marginal in the ecological gradients. This study highlights a case of strong population distinctiveness within a narrow range. Phylogeography sheds light on the historical role of the areas centrally situated in the distribution. The current range size and abundance patterns are not sufficient to predict the organization of genetic diversity.

Species diversity and abundance of ticks in three habitats in southern Italy

A 2-year study was conducted from March 2010 to March 2012 in a forested area in southern Italy to evaluate the species diversity and abundance of free-living ticks in 3 different habitats: (i) a meadow habitat within an enclosure inhabited by roe deer (Capreolus capreolus); (ii) a man-made trail located in a high-altitude, forested area; and (iii) a grassland near a house inhabited by 3 people. In total, 10,795 ticks were collected. Ixodes ricinus was the most abundant species (69.0%), followed by Haemaphysalis inermis (19.1%), Rhipicephalus turanicus (6.7%), Dermacentor marginatus (3.2%), and Hyalomma marginatum (1.0%). The least frequently collected species were Rhipicephalus bursa, Haemaphysalis parva, Haemaphysalis sulcata, and Haemaphysalis concinna, representing together less than 1% of the collections. Immature ticks predominated over adult ticks. In particular, immature stages of Ix. ricinus (i.e., 3246 larvae and 3554 nymphs) represented 63% of the total number of ticks collected. High levels of species diversity and abundance of ticks were recorded in all habitats and the daily number of ticks collected was negatively correlated with daily mean temperature, evapotranspiration, and saturation deficit. This study indicates that the southern Italian climate is suitable for different tick species, which may find a preferred ‘climate niche’ during a specific season, when a combination of factors (e.g., suitable meteorological and environmental conditions) associated with the presence of suitable hosts will facilitate their development and reproduction.

Spatial and temporal scales of environmental forcing of Acartia populations (Copepoda: Calanoida) in the Canal de Mira (Ria de Aveiro, Portugal)†

Abstract Zooplankton and hydrological data were collected from August 2000 and June 2002 at six stations distributed throughout Canal de Mira (Ria de Aveiro, Portugal). The abundance of Acartia clausi and Acartia tonsa adults and juveniles of Acartia spp. for each station and month were combined in a three-way data matrix, which was decomposed into three two-way matrices corresponding to different modes: biological, time, and space. Cluster analysis applied on the space mode revealed the existence of three different zones as a consequence of zooplankton composition. At each mode, principal component analysis showed strong seasonal variations in zones 1 and 2. A different spatial pattern was found between the periods November 2000–April 2001 and November 2001–April 2002, with the displacement of the highest abundance levels from the middle estuary to near the mouth. The congeneric populations were segregated in space: the A. clausi population was restricted to downstream stations (zone 1), whereas the A. tonsa population dominated the middle estuary. Significant correlations between hydrological parameters and copepod abundance were found to differ from zone to zone. The statistical methodology was a valuable tool to (i) discriminate spatial and seasonal distribution patterns, (ii) define estuarine sections based on the faunistic composition, and (iii) evaluate delayed effects of phytoplankton.

Comparison Between Maximum Sustained Yield Proxies and Maximum Sustained Yield

Attaining maximum sustained yield (MSY) is a central goal in U.S. fisheries management. To attain MSY, fishing mortality is maintained at FMSY and biomass at BMSY. Replacing FMSY and BMSY by “proxies” for FMSY and BMSY is commonplace. However, these proxies are not equivalent to FMSY and BMSY. The lack of equivalency is an important issue with regard to whether MSY is attained or whether biomass production is wasted. In this paper we study the magnitude of the equivalency. We compare FMSY/BMSY (calculated using the ASPIC toolbox) with the proxy estimates, F40%/B40%, published in GARM III. Our calculations confirm that in general the FMSY/BMSY calculations differ from the GARM III proxy estimates. The proxy estimates generally indicate that the stocks are overfished and are at relatively low biomasses, while the ASPIC estimates generally reflect the opposite: the stocks are not overfished and are at relatively high levels of abundance. In comparing the two approaches, the ASPIC estimates appeared favorable over the proxy estimates because 1) the ASPIC estimates involve only a few parameters in contrast to the many parameters estimated in the proxy approach, 2) “real variance” estimates for the proxy are not available so that it is difficult to evaluate the statistical adequacy of the proxy approach relative to the ASPIC approach, and 3) the proxy approach is based on many components (e.g., growth, stock and recruitment, etc.) that are subject to considerable uncertainty.

CARACTERÍSTICAS OCEANOGRÁFICAS Y BIOLÓGICAS DE LAS COMUNIDADES DEL PLANCTON Y NECTON DEL ÁREA MARINA COSTERA PROTEGIDA FRANCISCO COLOANE: UNA REVISIÓN

The objective of this study was to make a review of the current state of knowledge on the Coastal Marine Protected Area (CMPA) Francisco Coloane in terms of the physical and chemical oceanography, the phyto-zooplankton compositions and the fish, birds and marine mammals species present, and provide temperature and salinity data collected during a marine survey carried out the 2012 fall season. Surface temperatures fluctuate between 2.0 and 9.0°C. Salinity varies between 23 and 30.9 PSU. The most oxygenated waters and those of highest pH were recorded within Seno Ballena (320-340 µmoles Kg -1 ; 8.11 units of pH). In spring nutrient levels indicate low surface concentrations, except for phosphate, the phytoplankton is dominated by diatoms and the zooplankton is composed of copepods and meroplanktonic larvae with high abundance levels in the Jeronimo Channel. The family of fish most representative is the Nototheniidae, and among marine birds and mammals, the Magellan penguin and the humpback whale are the most sighted. The review indicates that it is necessary to make further studies on physical oceanography and the benthic community of CMPA.