A total of 300 clinical isolates of enterococci, collected from patients admitted at Srinagarind hospital, were investigated for antimicrobial resistance and species distribution. Antimicrobial susceptibility to 10 antimicrobial agents was performed by disc diffusion method. Screening for vancomycin resistance was performed by the agar plate method and minimal inhibitory concentration of vancomycin was determined for vancomycin resistant strains by microbroth dilution method. Enterococcus faecalis was found to be predominant species (58.7%) followed by E. faecium (35.7%) and the rest species (5.7%) including E. hirae, E. gallinarum, E. durans and E. dispar. The isolates were resistant to penicillin (51.3%), ampicillin (43.3%), high level gentamicin (57.7%), azithromycin (100%), chloramphenicol (16.3%), doxycycline (48%), quinupristin/dalfopristin (53%) and linezolid (8%). None of the isolates were resistant to teicoplanin. Five (1.7%) strains were resistant to vancomycin. The minimal inhibitory concentrations of vancomycin for vancomycin-resistant enterococci strains were 16∝g/ml. E. faecium was more resistant to penicillin, ampicillin and high level gentamicin than E. faecalis. Two hundred and seventy four (91.3%) strains showed multidrug resistance. Our study showed low prevalence of vancomycin resistance. However, there is a need to carry-out regular surveillance of antimicrobial resistance of enterococci to monitor changes in their patterns to prevent the spreading of resistant isolates.