Higher Lactic Acid Bacteria Counts Research Articles

Effect of Lactiplantibacillus plantarum CNPC003 and milk pasteurization on artisanal goat coalho cheese characteristics

This study evaluated the effect of adding Lactiplantibacillus plantarum CNPC003 (LP) and applying milk pasteurization on the microbiological, technological, and physicochemical characteristics and protein profile of artisanal goat coalho cheese during 60 days of ripening. Four types of cheese were produced, including cheeses made with raw and pasteurized goat milk and with and without LP (RC, PC, RCLP, and PCLP). All cheeses were safe for consumption and maintained high lactic acid bacteria counts (above 8 log CFU mL−1) at the end of the ripening period. RC and RCLP showed higher hardness and lower chewiness than cheeses made with pasteurized milk (PC and PCLP) after 60 days of ripening. As expected, there was a reduction in lactose content with a simultaneous increase in acidity and syneresis for all cheeses over the 60 days of ripening, with no significant change in the other evaluated physicochemical parameters. Proteolysis rates increased in all cheeses up to 60 days of ripening, aligning with elevated levels of soluble protein and free amino acids. After 60 days of ripening, PC showed a higher extent of proteolysis index, higher amount of high molecular weight peptides, higher number of identified proteins, and lower content of free amino acids. RCLP showed a higher depth of proteolysis index, higher free amino acids amount (1583 ± 3.95 mg 100 g−1), and fewer identified proteins. Among the peptides identified, it was possible to define 30 potential bioactive peptides according to the bioactivity score (>0.5), where both cheeses added with LP had 18 of these peptides, mostly potentially ACE inhibition sequences. The addition of LP and the pasteurization of milk positively affected the microbiological safety of ripened coalho goat cheeses, primarily contributing to the differentiation of their texture and protein and bioactivity profile.

Effect of locally available and commercial preservatives on nutrient content, organic matter digestibility and microbial changes of wet brewers' grain

The aim of the study was to evaluate the effect of different preservatives on nutrient content, organic matter digestibility (OMD), ammonia nitrogen (NH3-N), short-chain fatty acid (SCFA), yeast, mold, and lactic acid bacteria (LAB) concentrations of wet brewery grain (WBG). The experiment was undertaken for 28-days using five treatments (T); T1 = no preservative; T2 = 1.75% salt; T3 = 2.5% molasses; T4 = 2.5% Effective Microbial (EM) and T5 = 0.6% formic acid. Data was analyzed using a completely randomized two way factorial design of the mixed procedure of SAS (Version 9.1, 2001). The highest dry matter loss was for WBG stored with T1, while the lowest was for WBG treated with T5. WBG treated with T5 had the highest crude protein content, while those with T1 had the lowest. OMD of WBG treated with T5 was higher than T4. SCFA content of WBG treated with T3 was higher than those T4. WBG stored with T1 and T3 had a higher NH3N content than T4 and T5. WBG stored with T1 had the highest yeast and mold concentration, while WBG treated with T5 had the least. The highest LAB count was recorded for WBG treated with T5, while the least was for T1 and T3. Results has shown that preserving WBG with 0.6% formic acid is found to be more effective in terms of preventing spoilage by inhibiting mold and yeast growth as compared to other preservatives used in this study.

Microbiological quality and safety of vacuum-packaged white-tailed deer meat stored at 4 °C

Vacuum packaging is widely used for extending the shelf life of commercial fresh meat products. It also ensures product hygiene during distribution and storage. However, very little information exists concerning the effects of vacuum packaging on the shelf life of deer meat. One of our aims was to evaluate how storage under vacuum at 4 °C affects the microbial quality and safety of white-tailed deer (Odocoileus virginianus) meat cuts. This was assessed in a longitudinal study based on sensory analyses and measurements of (1) mesophilic aerobic bacteria (MAB), (2) lactic acid bacteria (LAB), (3) enterobacteria (EB), (4) and Escherichia coli (EC) counts, and the presence of foodborne pathogens (Campylobacter, Salmonella, stx-harbouring E. coli (STEC), Yersinia and Listeria). Microbiomes were additionally investigated by 16S rRNA gene amplicon sequencing at the time of spoilage. In total, 50 vacuum-packaged meat cuts from the carcasses of 10 wild white-tailed deer harvested in southern Finland in December 2018 were analysed. A significant (p < 0.001) drop in the odour and appearance scores and a significant increase in MAB (p < 0.001) and LAB (p = 0.001) counts of the vacuum-packaged meat cuts were observed after 3 weeks of storage at 4 °C. A very strong correlation (rs = 0.9444, p < 0.001) between the MAB and LAB counts were found during the 5-week sampling period. Clear spoilage changes, manifested as sour off-odours (odour scores ≤2) and pale colour, were detected in the meat cuts spoilt after 3-week storage. High (≥8 log10 cfu/g) MAB and LAB counts were also detected. According to the 16S rRNA gene amplicon analyses, Lactobacillus was the dominant bacterial genus in these samples, demonstrating that LAB can cause rapid spoilage of vacuum-packaged deer meat cuts stored at 4 °C. The rest of the samples were spoilt after four or five weeks of storage, and a vast number of bacterial genera were identified in them. Listeria and STEC were detected by PCR in 50 % and 18 % of the meat cut samples, respectively, which may indicate a public health problem. Our results demonstrate that it is very challenging to ensure the quality and safety of vacuum-packaged deer meat stored at 4 °C, and freezing is therefore recommended to prolong the shelf life.

Sheep's Butter and Correspondent Buttermilk Produced with Sweet Cream and Cream Fermented by Aromatic Starter, Kefir and Probiotic Culture.

Small ruminant dairy products are common in some Mediterranean countries, in the Middle East and Africa, and can play a particular role in the development of rural areas. Butter has been the object of few research studies aimed at evaluating its potential as a vehicle for probiotic microorganisms. Moreover, the recovery of fermented buttermilk with functional properties can be considered an excellent opportunity to value this dairy byproduct. Therefore, the purpose of the present work was to develop different sheep butters and respective buttermilks after cream fermentation by: (1) a mesophilic aromatic starter (A); (2) a kefir culture (K); and (3) a mixture of probiotic bacteria (P). The butters and buttermilk produced with fermented cream were compared with non-fermented sweet cream (S) butter or buttermilk, respectively, regarding their physicochemical, microbiological and sensory characteristics. The adjusted production (%, w/v) obtained for butter were: S (44.48%), A (36.82%), K (41.23%) and P (43.36%). S, A and K butters had higher solids, fat and ashes contents than P butter. The probiotic butter had a total fat of ca. 75% (w/w), below the legal limits, while all others had fat levels above 81.5%. In all samples, the pH decreased and the acidity increased over 90 days of refrigerated storage. These variations were more evident in the P butter, which agrees with the highest lactic acid bacteria counts found in this sample. Differences in color between samples and due to storage time were also observed. In general, the butter samples tended to become darker and yellower after the 60th day of storage. Texture analysis showed comparable results between samples and greater hardness was observed for the P butter, most probably due to its higher relative saturated fatty acids content (66.46% compared to 62-64% in S, A and K butters). Regarding rheological properties, all butters showed pseudoplastic behavior, but butter P had the lowest consistency index (249 kPa.sn-1). The probiotic butter and the corresponding buttermilk had viable cell counts greater than 7 Log CFU/g, indicating their suitability as probiotic carriers. All products were well accepted by consumers and small, but non-significant, differences (p &gt; 0.05) were observed in relation to the sensory parameters evaluated. In general, it can be concluded that the use of adequate starter cultures can allow the production of innovative and potentially healthier products, alongside the valorization of dairy byproducts, improving the income of small-scale producers.

Effect of Salt Addition and Fermentation Time on Phenolics, Microbial Dynamics, Volatile Organic Compounds, and Sensory Properties of the PDO Table Olives of Gaeta (Italy).

'Oliva di Gaeta' is almost certainly the most important and well-known PDO denomination for table olives in Italy. Their production is based on a specific two-stage trade preparation called the 'Itrana' method. In this work, we investigated how variations in the duration of the initial water fermentation (i.e., 15 and 30 days) and the salt concentration (i.e., 6% and 8% NaCl) influence the chemical features, microbial dynamics, polyphenols, volatile organic compounds, and sensory features of 'Oliva di Gaeta'. The time of the addition of salt did not affect the final concentration in the brine, but a longer initial water fermentation (before salt addition) led to lower pH values. The bacterial count constantly increased until the salt addition (i.e., either 15 or 30 days), while the yeast population peaked on day 30. Generally, the two different salt concentrations did not affect the count of microorganisms at the end of fermentation, with the only exception being a higher lactic acid bacteria count for the treatment with 6% salt added at 30 days. At commercial maturity, the crucial bitter tastant oleuropein was not completely removed from the drupes, and differences in salt concentration and the length of the first-stage water fermentation did not influence its content at the end of olive curing. Richer volatile profiles of olives were detected with higher-salt treatments, while the combination of low salt and early saline treatment provided a more distinct profile. Longer initial water fermentation caused a small increase in some phenolic compounds (e.g., iso-verbascoside, verbascoside, and hydroxytyrosol-glucoside). A panel test indicated that salt application at 30 days resulted in a more "Sour" and "Bitter" taste, irrespective of the salt concentration. The low salt concentration coupled with the late saline treatment resulted in more "Fruity" notes, probably due to the higher production of esters by lactobacilli. The slightly bitter perception of the olives was consistent with the partial removal of oleuropein. Our work revealed the characteristics of the 'Itrana' method and that the variation in salt concentration and its time of application changes parameters ranging from the microbial dynamics to the sensory profile. Specifically, our data indicate that 6% NaCl coupled with a longer initial water fermentation is the most different condition: it is less effective in blocking microbial growth but, at the same time, is more potent in altering the nutritional (e.g., polyphenols) and sensorial qualities (e.g., bitterness and fruitiness) of 'Oliva di Gaeta'.

Host-derived bacillus spp. as probiotic additives for improved growth performance in broilers

In recent years, the utilization of antibiotics in animal feed has been restricted, probiotics have been increasingly used to replace antibiotics in maintaining animal health. The aim of this study was to screen and evaluate probiotics with excellent probiotic potential from the gut of healthy goslings for clinical application. Thirteen strains of Bacillus (named AH-G201 to AH-G2013), including 2 strains of Bacillus subtilis (B. subtilis), 6 strains of Bacillus licheniformis (B. licheniformis) and 5 strains of Bacillus amyloliquefaciens (B. amyloliquefaciens), were isolated and identified. Then, acid and bile salts tolerance tests were performed to screen probiotics strains that could survive under different environments. The effects of screened probiotics on the growth of pathogenic Escherichia coli (E. coli) and Salmonella were assessed. Furthermore, we performed the drug resistance tests and safety tests in animals. The results showed that B. Subtilis AH-G201, B. licheniformis AH-G202 and AH-G204 exhibited higher gastrointestinal resistance under in vitro conditions, and showed a moderate level of resistance to the tested antibiotics. Importantly, AH-G201 and AH-G202 showed 24 to 60% inhibition rate against pathogenic E. coli and Salmonella. Moreover, the safety analysis of AH-G201 and AH-G202 suggested that the 2 probiotics strains have no adverse effects on body weight gain and feed intake in the broilers, and in addition, they have significantly improved growth performance. Finally, we analyzed effects of B. Subtilis AH-G201and B. licheniformis AH-G202 on growth performance, immune organ index and the feces microbes of broilers. The results showed that broilers fed with high doses (5×109 CFU/mL, for single strain) of a mixture of AH-G201 and AH-G202 exhibited good growth performance, and exhibited the greatest gain in spleen weight and the highest lactic acid bacteria counts. These findings indicate that the combined addition of B. Subtilis AH-G201 and B. licheniformis AH-G202 has the potential to replace antibiotics and to improve the growth performance of broilers.

The amount of lactic acid bacteria in fermented natural lactic acid bacteria liquids prepared with varying sucrose inclusion at different incubation periods

Characterization of lactic acid bacteria that naturally occurs during silage formation is important for determining the quality of silage feeds which are required in terms of meeting the need for high quality roughage in cattle breeding. In this study, the aim was to determine the total lactic acid bacteria (LAB) count and pH values of fermented natural LAB liquids as a result of different levels of sucrose addition as microbial silage additive (1%, 3%, 5% and 10%) and different incubation periods (5, 7, 9, 11, 13 and 15 days). Considering the incubation periods of LAB liquids, while the highest LAB count was obtained from the 10% sucrose added groups (3.7x1011, 2.6x1011 and 2.53x1012 colony forming unit (cfu/ml) at the 7, 9 and 13 days of incubation periods, the highest LAB count was obtained from the 5% sucrose supplemented group (9.13x1011cfu/ml) at the end of the 15-day incubation period. In general, for each incubation period, the total LAB counts in the obtained fluids increased due to the increase in the sucrose level (P&lt;0.05). Depending on the increase in the incubation period, there was an increase in the total LAB counts in the 10% sucrose supplemented group, while a decrease in the total LAB counts occurred after a 15-day incubation period. In general, pH values decreased depending on the increasing with sucrose levels in each incubation period. Considering the pH values of the LAB liquids, there were no differences of pH values depending on the increase the incubation period from the 1% and 3% sucrose added groups (P&lt;0.05). The pH values obtained from the 10% sucrose added group decreased with the increasing incubation periods. The highest pH value was obtained from the 1% sucrose group (4.49) during the 15-day incubation period, whereas the lowest pH value (3.78) was obtained from the 10% sucrose group in the same incubation period. Considering the LAB counts (1011-12cfu/ml) in the study, it has been evaluated that the obtained LAB liquids can be used as silage additives and it has a high potential of commercialization.

Effects of dietary nano-selenium supplementation on broiler chicken performance, meat selenium content, intestinal microflora, intestinal morphology, and immune response

BackgroundSelenium (Se) is an essential micronutrient that is important to both animals and humans. Furthermore, scientists are increasingly interested in boosting the Se content of food products for human consumption, such as Se-enriched meat, because it has been shown to have a positive effect on human health. The purpose of this study was to compare the effects of green synthesized nano selenium (GNS) on broiler chicken growth performance, meat selenium content, intestinal microflora, intestinal morphology, and immune response to sodium selenite (SS) as inorganic Se. MethodsA total of 360 one-day-old Ross 308 broiler chickens were randomly assigned to four experimental groups, each of six replicates of 15 birds. The experimental treatments were as follows: (1) based diet supplemented with 0.15 mg/kg of SS (control group), (2–4) basal diet supplemented with 0.075, 0.15 and 0.3 mg/kg of GNS respectively. ResultsThe results showed that different experimental diets had no significant effect on growth performance. Meat Se content increased with dietary GNS supplementation (P < 0.05). The birds fed dietary supplements GNS compared to SS had higher lactic acid bacteria counts and lactic acid bacteria/coliform ratios in ileum on day 42 (P < 0.05). Furthermore, as compared to SS, dietary supplementation of 0.3 mg GNS/kg significantly reduced coliform numbers in the cecum on day 42 (P < 0.05). Supplementation of 0.3 mg GNS/kg in the diet vs. SS at 21 days, there was a significant increase in VH/CD in the ileum and jejunum, as well as villus height and villus surface area in the ileum, and a decrease in crypt depth and epithelial cell layer thickness in the jejunum. Furthermore, at 42 days, birds fed 0.3 mg GNS/kg had higher villus height, villus surface area, and goblet cell density in the ileum and jejunum than birds fed SS (P < 0.05). Compared to SS, broilers fed 0.3 mg GNS/kg dietary supplementation had higher IgG at 42 days (P < 0.05). ConclusionIn comparison to the use of SS, it could be concluded that the inclusion of GNS as a novel Se source can improve meat Se content, intestinal microflora, intestinal morphology, and immune response.

Mediterranean Spontaneously Fermented Sausages: Spotlight on Microbiological and Quality Features to Exploit Their Bacterial Biodiversity.

The wide array of spontaneously fermented sausages of the Mediterranean area can represent a reservoir of microbial biodiversity and can be an important source of new technological and functional strains able to preserve product properties, counteracting the impoverishment of their organoleptic typical features due to the introduction of commercial starter cultures. We analysed 15 artisanal salamis from Italy, Spain, Croatia and Slovenia to evaluate the microbiota composition, through culture-dependent and culture-independent techniques (i.e., metagenomic analysis), chemical–physical features, biogenic amines and aroma profile. The final pH varied according to origin and procedures (e.g., higher pH in Italian samples due to long ripening and mold growth). Lactic acid bacteria (LAB) and coagulase-negative cocci (CNC) were the dominant population, with highest LAB counts in Croatian and Italian samples. Metagenomic analysis showed high variability in qualitative and quantitative microbial composition: among LAB, Latilactobacillus sakei was the dominant species, but Companilactobacillus spp. was present in high amounts (45–55% of the total ASVs) in some Spanish sausages. Among staphylococci, S. epidermidis, S. equorum, S. saprophyticus, S. succinus and S. xylosus were detected. As far as biogenic amines, tyramine was always present, while histamine was found only in two Spanish samples. These results can valorize the bacterial genetic heritage present in Mediterranean products, to find new candidates of autochthonous starter cultures or bioprotective agents.

Evaluation of the Microbial Load and pH of Pap Produced from Selected Cereals

The aim of this work was to evaluate the microbial load of pap samples made from three commonly utilized cereal grains namely: maize (Zea mays), millet (Pennisetum typhoideum) and guinea corn (Sorghum bicolor). Exactly 2 kg each of the aforementioned grains was steeped in 1000 ml of clean water for 3 days at 30±20C temperature. The water was disposed and the cereal grain separately washed thoroughly using clean water. The grains were then wet milled and the resulting paste mixed with1000 ml of clean water. The slurry was filtered with the aid of the muslin cloth. The filtrate was then allowed to stand for a period of 24 hours and the supernatant disposed. Microbiological analysis to determine microbial load on the various samples were carried using standard procedures. Guinea corn based pap had the highest lactic acid bacteria count of 9×103 CFU/g at 0 hr of fermentation which decreased to 6.6×105 CFU/g at the 72nd hr. On the other hand, the composite of the three cereals had the lactic acid bacteria count of 4.2×104 CFU/g at 0 hr of fermentation which increased to 8.6×105 CFU/g at the 72nd hr, the highest value recorded for all samples studied. Total coliform count on all samples maintained a uniform trend of increase at the 24th and 48th hr and a decrease at the 72nd hr. Millet based pap had the highest fungi count of 2.3×101CFU/g at 0hr of fermentation which increased progressively to 4.9×101 at the 72nd hr. However, pap derived from the composite of the cereals presented the lowest fungi count of 0.52×102 at 0hr which however increased progressively to record the highest count of 1.4×101at the 72nd hr. In conclusion, there is need to optimize the growth of lactic acid bacteria in the production of pap, this may enhance its acid production potential and consequent inhibition and/destruction of potential harmful microorganisms in fermented foods.

Assessment of Intestinal Immunity and Permeability of Broilers on Partial Replacement Diets of Two-Stage Fermented Soybean Meal by Bacillus velezensis and Lactobacillus brevis ATCC 367.

Simple SummaryInflammation induced by diet, environmental factors, and stimulated innate immunity is not conducive to intestinal maintenance and remodeling. Fermented soybean meal (FSBM) by Bacillus velezensis (Bv) and Lactobacillus brevis ATCC 367 (Lb) reduces these negative factors and provides bioactive active peptides that are beneficial to intestinal repair and regulate the immune system of the intestinal tract. This study showed that two-stage FSBM regulates the immunity and tight junction in the jejunum, which are beneficial to health and performance.The effect of soybean peptides from fermented soybean meal on the intestinal immunity and tight junction of broilers was assessed. Roughly, two-stage fermented soybean meal prepared with Bv and Lb (FSBMB+L), which has nearly three times higher soluble peptides than soybean meal (SBM), and reduced galacto-oligosaccharide (GOS) content and allergen protein. The one-stage fermented by Bv (FSBMB) has the highest soluble peptides, while commercial lactic acid bacteria (FSBML) has the highest Lactic acid bacteria count; these were used to compare the differences in the process. Ross308 broilers (n = 320) were divided into four groups: SBM diet and a diet replaced with 6% FSBMB+L, FSBMB, or FSBML. The growth performance was recorded during the experiment, and six birds (35-day-old) per group were euthanized. Analysis of their jejunum and ileum showed that the fermented soybean meal significantly improved the villus height in the jejunum (p < 0.05) and reduced the crypt hyperplasia. The FSBMB group had the highest reducing crypt depth; however, the FSBMB+L group had the highest villus height/crypt depth in the ileum (p < 0.05). In the jejunum, the relative mRNA of CLDN-1 and Occludin increased 2-fold in the treatments, and ZO-1 mRNA increased 1.5 times in FSBML and FSBMB+L (p < 0.05). Furthermore, the level of NF-κB and IL-6 mRNAs in FSBML increased, respectively, by 4 and 2.5 times. While FSBMB, along with FSBMB+L, had a 1.5-fold increase in the mRNA of IL-10, that of NF-κB increased 2-fold. FSBMB+L and FSBMB singly led to a 2- and 3-fold increase in IL-6 mRNA, respectively (p < 0.05). FSBMB and FSBMB+L can also upregulate MUC2 in the jejunum (p < 0.05). In short, using the soybean peptides from two-stage fermented soybean meal can ameliorate the negative factors of SBM and effectively regulate immune expression and intestinal repair, which will help broilers maintain intestinal integrity.

Influence of pre-treated bitter orange albedo on the physicochemical, textural and sensory properties of fermented sausages (sucuk).

The aim of this study was to determine the physicochemical (pH, water activity, titratable acidity, moisture, protein, fat, colour, weight loss and oxidative stability), textural and sensory properties, and the growth of lactic acid bacteria in fermented sausages(sucuk) supplemented with different levels (0, 1, 2.5 and 5.0%) of pre-treated bitter orange (Citrus aurantium) albedo. Results indicated that adding albedo to sucuk samples increased (P < 0.01) the titratable acidity, lightness and yellowness values; while it led to a decrease (P < 0.01) in pH and weight loss values. Moreover, it was observed that there were increases (P < 0.05) in Thiobarbituric acid(TBA) values when albedo was added to the samples. The highest lactic acid bacteria count was observed in the sucuk samples supplemented with 5% albedo. Regarding textural properties, albedo addition increased (P < 0.01) hardness, springiness, gumminess and chewiness values. Lastly, sensory analysis results showed that albedo added sucuk samples generally got higher (P < 0.05) scores from panellists compared to control samples.

Effect of starter culture type, cooking process and storage time at −18 °C on chemical, color and microbiological qualities of fermented sucuk doner kebab

The aim of this study was to produce fermented sucuk doner kebab and investigate the effects of starter culture type, heat treatment (82 °C) and storage time (0, 30, 90, 180 days) at −18 °C on pH, total acidity, thiobarbituric acid, residual nitrite-nitrate, color and microbiological quality. In this study, three types of starter cultures were used. These were commercial starter culture, L.sakei + S.xylosus + L.curvatus, isolated starter culture (1), L.sakei + S.xylosus + L.curvatus and isolated starter culture (2), L.sakei + S.xylosus + L.plantarum. There was also control product which contains no starter culture. The major pH decrease was observed in samples containing isolated starter cultures. Cooking and storage caused an increase in pH. Isolated starter culture was more effective than commercial starter culture in reducing residual nitrite-nitrate. Isolated starter cultures caused an increase in L* color values. The highest lactic acid bacteria count was found in sample containing commercial starter culture. Cooking was effective in inhibiting Enterobacteriaceae.

GROWTH PERFORMANCE AND MICROFLORA IN GROWING QUAIL FED DIETS SUPPLEMENTED WITH Lactuca serriola POWDER

The aim of the current study was to assess the influence of Lactuca serriola supplemented diet on performance, carcass characteristics and intestinal microbiota count of Japanese quails. A total of eighty birds of quail (one week of age) were divided into 2 groups of 40 birds each (five replicates of eight birds each). In the first group, the birds were fed a basal diet without Lactuca serriola, while the birds in the second group were fed diets containing 20 g Lactuca serriola /kg feed. Birds in both groups were fed for five weeks. Quails were slaughtered in order to study intestinal microbiota count and carcass characteristics at six weeks age. The results showed that quails fed Lactuca serriola had higher growth performance including body weight in comparison with the control group. The inclusion of Lactuca serriola had significantly higher lactic acid bacteria count and lowest level of E. coli and Salmonella enterica count than the control group. However, Lactuca serriola did not affect carcass measurements. Thus, supplementation of Lactuca serriola can improve the performance of Japanese quail and used as a new feed additive to balance gut microbiota

Silage Fermentation, Bacterial Community, and Aerobic Stability of Total Mixed Ration Containing Wet Corn Gluten Feed and Corn Stover Prepared with Different Additives.

Simple SummaryWet corn gluten feed (WCGF) is a feed containing high moisture and rapidly digestible, non-forage fiber and protein for dairy cows, that is difficult to preserve. The use of corn stover as roughage by ruminants is limited by its poor digestibility. Total mixed ration (TMR) silage is an ensiling mixed feed that can fully mix forage and concentrate in a specific ratio to satisfy the nutritional needs of dairy cows, which has become an effective method of preservation of high-moisture byproducts. The objective of this study was to investigate the effects of different additives on the fermentation quality, bacterial community, and aerobic stability of TMR silage containing WCGF and corn stover. Inoculation with lactic acid bacteria (LAB) + fibrolytic enzyme (EN) and LAB improved aerobic stability of TMR silages indicated by higher and more stable LA and AA contents, a smaller rise in pH, and yeast count than other silages. Total mixed ration silage inoculated the LAB + EN and LAB can become an effective method of preserving high-moisture WCGF and corn stover with poor digestibility.The objective of this study was to investigate the effects of different additives on the fermentation quality, bacterial community, and aerobic stability of total mixed ration (TMR) silage containing wet corn gluten feed (WCGF) and corn stover. The TMR was ensiled with four treatments: (1) no additive (control); (2) lactic acid bacteria (LAB); (3) fibrolytic enzyme (EN); (4) LAB + EN. The EN and LAB + EN decreased the neutral detergent fiber and acid detergent fiber contents. Additives led to a higher lactic acid (LA) content (p < 0.0001) compared to control at all ensiling times. Silages inoculated with LAB and LAB + EN had higher dry matter (p = 0.0007), LA (p < 0.0001) and acetic acid (AA) contents (p < 0.0001) compared to control. The LAB and LAB + EN had significantly lowest ammonia nitrogen among the treatments, while no significant difference occurred after days 7 of ensiling. Silages treated with LAB and LAB + EN had a higher LAB count (p < 0.0001) and a lower pH, yeast, and mold counts compared to other silages. The LAB and LAB + EN greatly increased the portions of Firmicutes and Lactobacillus (p < 0.0001, and p < 0.0001, respectively) and reduced undesirable bacteria. Inoculation with LAB + EN and LAB improved aerobic stability of TMR silages indicated by higher and more stable LA and AA contents, smaller rise in pH, and yeast count than other silages. The LAB + EN and LAB reduced microbial diversity and improved the fermentation quality and aerobic stability of TMR silage containing WCGF and corn stover.

Analysis of main factors affecting silage fermentation of sorghum prepared with whole crop and stover in semiarid West Africa

Sorghum (Sorghum bicolor L.) is widely cultivated in semiarid West Africa as a combined crop for human consumption and animal feed. In order to address the shortage of ruminant feed in Burkina Faso, we studied the potential of preparing silage from sorghum at various growth stages and analysed the main factors affecting silage fermentation, including the microbial population, chemical composition and ensiling characteristics of sorghum prepared with and without microbial additives at three stages of development (soft dough; hard dough; physiological mature stover). After 90 d of ensiling, lactic acid bacteria (LAB) became the dominant microorganisms in silages. The silage from soft dough sorghum exhibited the highest LAB counts, water-soluble carbohydrate (WSC) and crude protein content. In addition, silage presented greater quality with higher (p < 0.05) lactic acid content and lower (p < 0.05) pH and ammonia nitrogen levels than those at hard dough stage and stover. The WSC and LAB were the important factors affecting silage fermentation and the addition of LAB and cellulase improved fermentation quality. The combined additives displayed a synergistic effect and when silage is prepared with sorghum at soft dough stage, it has best potential for controlling spoilage microorganisms and preserving the silage quality for animal feed.

EFFECT OF GINGER EXTRACT ON THE VIABILITY OF LACTIC ACID BACTERIA AND SENSORY CHARACTERISTICS OF DAIRY YOGHURT AND SOY YOGHURT

Yoghurt is usually produced from cow milk which is unsuitable for vegetarian diet. Chemical preservative used for its preservation may have adverse effect on the lactic acid bacteria. This research was designed to evaluate the effect of ginger extract on the viability of lactic acid bacteria, physico-chemical property and sensory attributes of bio preserved functional yoghurt produced from dairy milk and soy milk during storage at refrigerated temperature. The result obtained showed that yoghurt produced from soy milk (5.67 – 7.52 CFU/ml) had higher lactic acid bacteria count than yoghurt from dairy milk (4.71 – 7.31 CFU/ml) throughout the period of storage. Ginger extract was more active against fungi in dairy yoghurt than (0 - 5.09 CFU/ml) soy yoghurt (0 – 5.29 CFU/ml). The pH was generally lower in dairy yoghurt (4.29 - 4.67) than soy yoghurt (4.69 – 5.14) while the titratable acidity was higher in dairy yoghurt (1.30 – 2-01%) than soy yoghurt (0.7 – 1.54%). Addition of ginger extract improved the texture and flavour of soy yoghurt. It is therefore suggested that functional yoghurt with good sensory attributes can be produced from soy milk and dairy milk using ginger extract as bio-preservative.

Efeito da acidificação direta e microbiana sobre as propriedades químicas, físicas e microbiológicas do queijo de manteiga

O Queijo de Manteiga é um produto de grande consumo e valor socioeconômico para o Nordeste brasileiro. Para sua elaboração, a Legislação estabelecida que a coagulação do leite seja realizada com ingredientes orgânicos; no entanto, os pequenos produtores realizam essa etapa da ação da microbiota natural do leite. O objetivo deste trabalho foi avaliar o uso da acidificação microbiana com cultura iniciante tipo “LD”, comparativamente à acidificação direta com o uso de emulsificante, sobre as características químicas, químicas e microbiológicas do produto final. O experimento foi realizado em triplicata, representando processos independentes. Os controles não tiveram impacto sobre o teor de gordura dos queijos, mas o pH, a umidade e o teor de cinzas do queijo elaborado por acidificação direta superior (p≤0, 05) ao queijo elaborado por acidificação microbiana. O queijo elaborado por acidificação direta mostrou menos firme e menor mastigabilidade (p≤0,05). Todos os queijos atendem ao estabelecido pela IN n ° 60/2019, com contagem de coliformes fecais &lt;3,0 NMP / g, ausência de Salmonella sp e Staphylococcus spp &lt;10,0 UFC / g. No entanto, uma contagem de bactérias lácticas foi maior no queijo elaborado por acidificação microbiana. Por apresentar menor pH e maior número de bactérias, é possível que queijo de manteiga permita uma acidificação microbiana, maior segurança alimentar ou queijo elaborado por acidificação direta, dado que bactérias láticas, afetado pela produção de bacteriocinas, preconceito ou efeito inibidor. sobre o crescimento de bactérias patogênicas. Estudos futuros devem explorar esta hipótese.

Influence of the Fermented Feed and Vaccination and Their Interaction on Parameters of Large White/Norwegian Landrace Piglets.

Simple SummaryFarm animals are constantly exposed to pathogenic microorganisms, which can lead to bacterial, as well as secondary infections caused by viruses. For this reason, vaccination is used. However, many discussions have been published about the effectiveness of this prophylactic measure. The aim of this study was to evaluate the influence of fermented feed on non-vaccinated (NV) and vaccinated with Circovac porcine circovirus type 2 vaccine piglets’ blood parameters, gut microbial composition, growth performance and ammonia emission. The 36-day experiment was conducted using 25-day-old Large White/Norwegian Landrace piglets, which were randomly divided into four groups each comprising 100 piglets (NV piglets fed with soya meal, vaccinated piglets fed with soya meal, NV piglets fed with fermented rapeseed meal and vaccinated piglets fed with fermented rapeseed meal). The results revealed that vaccination, as a separate factor, did not significantly influence piglets’ blood parameters. Finally, rapeseed meal fermented with the selected lactic acid bacteria strains can be used instead of expensive imported soya, because the fermented feed did not cause undesirable changes in piglets’ health and growth performance. Furthermore, the process is more sustainable.The aim of this study was to evaluate the influence of fermented with a newly isolated lactic acid bacteria (LAB) strains combination (Lactobacillus plantarum LUHS122, Lactobacillus casei LUHS210, Lactobacillus farraginis LUHS206, Pediococcus acidilactici LUHS29, Lactobacillus plantarum LUHS135 and Lactobacillus uvarum LUHS245) feed on non-vaccinated (NV) and vaccinated with Circovac porcine circovirus type 2 vaccine (QI09AA07, CEVA-PHYLAXIA Co. Ltd. Szállás u. 5. 1107 Budapest, Hungary) piglets’ blood parameters, gut microbial composition, growth performance and ammonia emission. The 36-day experiment was conducted using 25-day-old Large White/Norwegian Landrace (LW/NL) piglets, which were randomly divided into four groups with 100 piglets each: SnonV—non-vaccinated piglets fed with control group compound feed; SV—vaccinated piglets fed with control group compound feed; RFnonV—non-vaccinated piglets fed with fermented compound feed; RFV—vaccinated piglets fed with fermented compound feed. Samples from 10 animals per group were collected at the beginning and end of the experiment. Metagenomic analysis showed that fermentation had a positive impact on the Lactobacillus prevalence during the post-weaning period of pigs, and vaccination had no negative impact on microbial communities. Although a higher amount of Lactobacillus was detected in vaccinated, compared with non-vaccinated groups. At the end of experiment, there was a significantly higher LAB count in the faeces of both vaccinated compared to non-vaccinated groups (26.6% for SV and 17.2% for RFV), with the highest LAB count in the SV group. At the end of experiment, the SV faeces also had the highest total bacteria count (TBC). The RFV group had a 13.2% increase in total enterobacteria count (TEC) at the end of experiment, and the SV group showed a 31.2% higher yeast/mould (Y/M) count. There were no significant differences in the average daily gain (ADG) among the groups; however, there were significant differences in the feed conversion ratios (FCR) between several groups: SV vs. SnonV (11.5% lower in the SV group), RFV vs. RFnonV (10.2% lower in the RFnonV group) and SV vs. RFV (21.6% lower in the SV group). Furthermore, there was a significant, very strong positive correlation between FCR and TEC in piglets’ faeces (R = 0.919, p = 0.041). The lowest ammonia emission was in RFV group section (58.2, 23.8, and 47.33% lower compared with the SnonV, SV and RFnonV groups, respectively). Notably, there was lower ammonia emission in vaccinated groups (45.2% lower in SV vs. SnonV and 47.33% lower in RFV vs. RFnonV). There was also a significant, very strong positive correlation between ammonia emission and Y/M count in piglets’ faeces at the end of the experiment (R = 0.974; p = 0.013). Vaccination as a separate factor did not significantly influence piglets’ blood parameters. Overall, by changing from an extruded soya to cheaper rapeseed meal and applying the fermentation model with the selected LAB combination, it is possible to feed piglets without any undesirable changes in health and growth performance in a more sustainable manner. However, to evaluate the influence of vaccination and its interaction with other parameters (feed, piglets’ age, breed, etc.) on piglets’ parameters, additional studies should be performed and methods should be standardised to ensure the results may be compared.

Formulation and development of composite fruit peel powder incorporated fat and sugar-free probiotic set yogurt

Reduction of milk fat and added sugar makes the yogurt even healthier, yet texture deformations and less consumer acceptability will be attributed. Fiber incorporation may be counteracting these problems, which is tested in the present study by incorporating underutilize and cheap fiber sources. Composite fruit peel powder (FPP) formulated from industrial waste was incorporated into fat and added sugar-free set yogurts in three different proportions as 0.5%, 0.7% and 1% (w/v). Yogurt without added FPP was considered as the control. Alteration of titrable acidity, pH and lactic acid bacteria viability were evaluated over a period of a month. Total soluble solids content (TSS), color values, texture profile, and consumer acceptability also were tested. Results showed that FPP incorporation reduces the initial pH of the yogurt mix while increasing the TSS and impart color changes. The elevated amount of FPP mixture (0.7% and 1%) adversely affect texture parameters, microbial count and sensory acceptability. Firmness increased, syneresis decreased, and comparatively high lactic acid bacteria counts observed in fat and sugar-free yogurt with 0.5% FPP incorporation. No significant difference (p