High-intensity Pulses Research Articles

High-intensity pulse magnetic therapy and drinking mineral water in rehabilitation treatment of patients with chronic pancreatitis at inpatient stage

BACKGROUND: Sanogenetic effects of physical factors, providing restoration of disturbed functions of the organism, are the basis for their inclusion in the standardised programme of restorative treatment of patients with chronic pancreatitis. AIM: To study the effectiveness of high-intensity pulse magnetic therapy and low-mineralized drinking mineral waters in the restorative treatment of patients with chronic pancreatitis at the inpatient stage. MATERIALS AND METHODS: There has been carried out an open-label, randomized, controlled, prospective study of 125 patients with chronic pancreatitis. The patients were divided into 3 groups using the simple randomization method. In the control group (n=40) the patients underwent restorative treatment in accordance with federal clinical guidelines; in the group of comparison (n=42) he patients were additionally prescribed low-mineralized drinking mineral water; in the main group (n=43) the patients had high-intensity pulse magnetic therapy in addition to the treatment of the group of comparison. The effectiveness of rehabilitation measures was monitored using a visual analogue scale, a carbolene test, a hospital scale of anxiety and depression. RESULTS: The statistical analysis showed that the inclusion of drinking mineral water and high-intensity pulse magnetic therapy contributed to a significant reduction of pain (p 0.01) and dyspeptic syndromes (p 0.01), an improvement in motor evacuation function of intestine (p 0.01), a decrease in enzymatic activity and the severity of anxiety-depressive manifestations (p 0.01). The effectiveness of the developed programmes of restorative treatment of the patients suffering from chronic pancreatitis was 87.8–92.5%, the standardized programme ― 78.5%. CONCLUSION: The inclusion of drinking mineral waters and high-intensity pulse magnetic therapy in the standardized program of restorative treatment of the patients suffering from chronic pancreatitis at the inpatient stage provides a significant restoration of pancreatic external secretory function.

An avalanche transistor-based Marx circuit pulse generator with sub-nanosecond, high frequency and high-voltage for pathogenic Escherichia coli ablation

Pathogenic Escherichia coli, which will easily cause environmental pollution, is a potentially hazardous microorganism that enters the environment through various routes such as biological feces. The presence of this bacterium poses a serious health risk, especially in water sources. When consuming water containing excessive amounts of pathogenic Escherichia coli, both humans and animals may experience severe health issues, such as diarrhea and gastrointestinal infections. However, current treatment methods for this bacterium are not ideal, and traditional means sometimes struggle to completely eliminate these stubborn microorganisms. In recent years, the scientific community has been exploring new sterilization techniques to address this challenge without causing secondary environmental pollution. Among them, pulsed electric field (PEF) technology has garnered significant attention. By applying high-intensity electric field pulses, PEF technology can have a fatal impact on microbial cells in a very short period of time. Studies have shown that this technology can cause cell membrane perforation, destroying the integrity of the cell and leading to cell death. This method is not only capable of decomposing organic pollutants, but also has a significant bactericidal effect. Nevertheless, despite the theoretical potential of PEF technology, there is still relatively little research on its application, especially in terms of treating pathogenic Escherichia coli. Further studies and experimental verification are needed. This article investigates the bioelectromagnetic effects of electric pulses on pathogenic Escherichia coli in sewage through a self-developed pulse source device (capable of providing a voltage range from 1.7 kV to 2.2 kV, with a rise time of 190 ps and a maximum repetition rate of 20 kHz). The feasibility of using a pulsed electric field to purify water bodies is verified in this report.

Impact of treatment trajectory on the thermal ablation rate and biological tissue volumetric lesion during irradiation by shock-wave focusing ultrasonic beam

Thermal ablation rates and the shapes of volumetric biological tissue lesion are compared in a numerical experiment, in which biological tissue is exposed to pulsed periodic shock-wave high intensity focused ultrasound. The comparison is performed across three different irradiation sequences of discrete foci placed uniformly within the target area.

Electric wind induced texturing for enhanced thermoelectric performance of p-type Mg3Sb2-based materials

High-intensity electric pulse treatment (EPT) can induce electric wind effect in materials, which facilitates the grain rearrangement and reorientation in polycrystalline samples. For the first time, this study employed the EPT technique to construct texture in Mg3.04Ag0.02Sb2 bulk compounds, and a highly conductive channel was produced to promote the charge carrier transport. This leads to a 53 % improvement in carrier mobility of the EPT sample (4#20) over the pristine one at room temperature. Additionally, EPT does not affect the carrier concentration of the material, making the EPT samples possess significantly improved electrical conductivities and untouched Seebeck coefficients. Consequently, a 36 % improvement in zT value is achieved for the EPT sample (4#20) at 723 K, compared to the pristine one. This work demonstrates that EPT technique is an effective approach for constructing textured Mg3Sb2-based materials, offering a valuable avenue for high thermoelectric performance in other potential materials via manipulating properties-related texture.

Laser Machining at High ∼PW/cm2 Intensity and High Throughput

Laser machining by ultra-short (sub-ps) pulses at high intensity offers high precision, high throughput in terms of area or volume per unit time, and flexibility to adapt processing protocols to different materials on the same workpiece. Here, we consider the challenge of optimization for high throughput: how to use the maximum available laser power and larger focal spots for larger ablation volumes by implementing a fast scan. This implies the use of high-intensity pulses approaching ∼PW/cm2 at the threshold where tunneling ionization starts to contribute to overall ionization. A custom laser micromachining setup was developed and built to enable high speed, large-area processing, and easy system reconfiguration for different tasks. The main components include the laser, stages, scanners, control system, and software. Machining of metals such as Cu, Al, or stainless steel and fused silica surfaces at high fluence and high exposure doses at high scan speeds up to 3 m/s were tested for the fluence scaling of ablation volume, which was found to be linear. The largest material removal rate was 10 mm3/min for Cu and 20 mm3/min for Al at the maximum power 80 W (25 J/cm2 per pulse). Modified surfaces are color-classified for their appearance, which is dependent on surface roughness and chemical modification. Such color-coding can be used as a feedback parameter for industrial process control.

High Power Pulsed LED Driver for Vibration Measurements.

Vibration measurements pose specific experimental challenges to be faced. In particular, optical methods can be used to obtain full-field vibration information. In this scenario, stereo-camera systems can be developed to obtain 3D displacement measurements. As vibration frequency increases, the common approach is to reduce camera exposure time to avoid blurred images, which can lead to under-exposed images and data loss, as well as issues with the synchronization of the stereo pair. Both of these problems can be solved by using high-intensity light pulses, which can produce high-quality images and guarantee camera synchronization since data is saved by both cameras only during the short-time light pulse. To this extent, high-power Light-Emitting Diodes (LEDs) can be used, but even if the LED itself can have a fast response time, specific electronic drivers are needed to ensure the desired timing of the light pulse. In this paper, a circuit is specifically designed to achieve high-intensity short-time light pulses in the range of 1 µs. A prototype of the designed board was assembled and tested to check its capability to respect the specification. Three different measurement methods are proposed and validated to achieve short-time light pulse measurements: shunt voltage measurement, direct photodiode measurement with a low-cost sensor, and indirect pulse measurement through a low-frame-rate digital camera.

The estimation of pore size distribution of electroporated MCF-7 cell membrane

ABSTRACT The size of the pores created by external electrical pulses is important for molecule delivery into the cell. The size of pores and their distribution on the cell membrane determine the efficiency of molecule transport into the cell. There are very few studies visualizing the presence of electropores. In this study, we aimed to investigate the size distribution of electropores that were created by high intensity and short duration electrical pulses on MCF-7 cell membrane. Scanning Electron Microscopy (SEM) was used to visualize and characterize the membrane pores created by the external electric field. Structural changes on the surface of the electroporated cell membrane was observed by Atomic Force Microscopy (AFM). The size distribution of pore sizes was obtained by measuring the radius of 500 electropores. SEM imaging showed non-uniform patterning. The average radius of the electropores was 12 nm, 51.60% of pores were distributed within the range of 5 to 10 nm, and 81% of pores had radius below 15 nm. These results showed that microsecond (µs) high intensity electrical pulses cause the creation of heterogeneous nanopores on the cell membrane.

Holocene paleoenvironmental history of Jackson Lake (Grand Teton National Park, USA) deduced from CHIRP seismic reflection and radiocarbon-dated sediment cores

Sedimentary records from Jackson Lake (Grand Teton National Park, Wyoming, USA) provide an opportunity to investigate the Late Quaternary tectonic and hydroclimatic history of a region known to have considerable earthquake hazard potential. Here, we present a new paleoenvironmental reconstruction for Jackson Lake using high-resolution Compressed High-Intensity Radar Pulse (CHIRP) seismic reflection data and sediment cores from Moran Bay, which is adjacent to the active Teton fault on the southwestern lake margin. Three seismic units (SU) are present within Moran Bay. The lowermost SU-1 defines the acoustic basement, which is characterized by a sharp reflector of variable relief above material with a very low signal-to-noise seismic response; direct sampling reveals this unit to be glaciogenic in origin. SU-2 contains diverse acoustic facies that onlap, downlap, and drape underlying strata. Prominent in SU-2 are faulted, folded, and slumped reflections with mixed amplitude characteristics, as well as reflections organized into prograding clinoforms, which suggest a history of deformation and slope instability in fluvio-deltaic and shallow lacustrine environments. A local unconformity separates SU-2 and SU-3, and above this boundary clinoform packages backsteps towards the hinterland. A bi-directionally tapering package of low-moderate amplitude, continuous to semi-continuous reflections onlap the clinoform; this unit was fully cored (13.8 m), allowing the unit boundary to be sampled and dated. Radiocarbon ages indicate that the core spans ca. 10.4 calibrated kiloyears before present (cal ka BP) to present. Retrogradational stacking of deltaic clinoforms are best explained by increased accommodation in Moran Bay around ca. 10 ka, consistent with a major earthquake and hanging wall subsidence on the Teton fault. Two turbidites identified in the strata align temporally (ca. 10.3 ± 0.4 cal ka BP and ca. 8.3 ± 0.3 cal ka BP) with earthquakes on the Teton fault that have been identified in terrestrial and lacustrine paleoseismic records. From ca. 10.4–6.6 cal ka BP core sediments are relatively rich in clay with highly variable magnetic susceptibility and organic carbon content. Deposition during this period was influenced by an evolving stream network and a relatively dry paleoclimate. From ca. 6.6–2.7 cal ka BP core sediments have higher sand and organic carbon contents, suggesting variable fluvial discharge and climate instability. The youngest core records produced a sharp increase in sedimentation rate, sand and organic carbon content, propelled by greater effective moisture, higher lake levels, and increasing watershed vegetation density. Based on clinoform rollover position, water levels at ∼2,046, ∼2,049, ∼2,053, and 2063 m asl characterized Moran Bay during the Holocene. Together, the seismic and core data records indicate a complex and dynamic Holocene lacustrine evolution controlled by fault motion and water level changes.

Numerical experiment of phase-matched ion-based high-order harmonic generation in the water-window x-ray spectral region via electromagnetic envelope equation.

We present a phase-matching scheme for efficient high-order harmonic generation in the water-window x-ray spectral region using a 405-nm driving pulse. A high-intensity pulse (∼1016 W/cm2) is used to produce He1+ ions as the target medium, increasing the cutoff photon energy to the water-window x-ray spectral region. By adjusting the driving pulse divergence, the positive dipole phase variation balances the negative plasma dispersion and geometrical phase shift, achieving phase matching. Using the electromagnetic envelope equation coupled with the Keldysh ionization model, numerical experiments identify the optimal conditions. Results show that the relative conversion efficiencies of the 95th harmonic (4.26 nm) and the 169th harmonic (2.4 nm) reach 66% and 79% of the perfect phase-matching conditions, respectively.

Experimental study on the physical mechanism of pulse noise in the tail of a recoilless gun

During the firing process of a recoilless gun, there is a high-intensity pulse noise phenomenon in its rear breech area. In order to obtain the physical mechanism of the pulse noise formation, this article designed experiments and used high-speed cameras and overpressure sensors to discover a “three peaks” phenomenon in the pulse noise at the shooter’s position. Using resistance strain sensors, the changes of strain with time in the chamber and nozzle expansion section of the recoilless gun during the firing process were recorded. By comparing the micro-strain data and overpressure data, the shock waves at the shooter’s position can be categorized into two types: internal shock waves and external shock waves. Internally, the opening of the blocking sheet initiates the formation of the initial shock wave, followed by the formation of a second pressure wave within the combustion chamber. The peak-to-peak interval between these two shock waves is nearly fixed, and the ratio of the dual peaks increases with the distance of the measurement position. The third shock wave forms externally to the nozzle, induced by the explosion of unburned propellant. As the weapon’s distance from the ground increases, the peak values of the overpressure data decrease, and the waveform of this explosion-induced shock wave transitions from a single-peak phenomenon to a double-peak phenomenon. This study can provide a reference for the study of the potential risk of injury to operators using single soldier recoilless guns and similar weapons.

Investigation of pulse burst characteristics in an LD end-pumped Er:Yb:Glass/Co:MALO microchip 1.5 μm laser

Burst mode lasers, characterized by a sequence of high-intensity pulses, are integral to applications such as the examination of turbulent flows and optical metrology. This study investigates a 1.5 μm passively Q-switched pulse burst microchip laser, end-pumped by a 940 nm laser diode. Utilizing Er:Yb:glass as the gain medium and Co:MALO for passive Q-switching, delivering a pulse width of 5.5 ms and a repetition rate of 10 Hz. When the initial transmittance of the saturable absorber was 93 % and the maximum absorbed pump energy reached 85 mJ, we successfully generated up to 12, 8, and 4 pulses within a burst at 1.5 μm. These results corresponded to cavity lengths of 12 mm, 13 mm, and 14 mm, respectively. By using a saturable absorber with an initial transmittance of 90.5 %, a higher single pulse energy and a narrower pulse width were realized. The narrowest pulse width was reduced to 6.2 ns, with a corresponding single pulse energy of 272 μJ. The peak power in the Er:Yb:glass/Co:MALO pulse burst laser reached an estimated 43.9 kW.This research provides valuable experimental and theoretical insights for the development of human eye-safe lasers with adjustable pulse sequences.

Irreversible Electroporation-Induced Inflammation Facilitates Neutrophil-Mediated Drug Delivery to Enhance Pancreatic Cancer Therapy.

Pancreatic cancer is a deadly disease with a five-year overall survival rate of around 11%. Chemotherapy is a cornerstone in the treatment of this malignancy, but the intratumoral delivery of chemotherapy drugs is impaired by the highly fibrotic tumor-associated stroma. Irreversible electroporation (IRE) is an ablative technique for treating locally advanced pancreatic cancer. During a typical IRE procedure, high-intensity electric pulses are released to kill tumor cells through the irreversible disruption of the cytoplasm membranes. IRE also induces rapid tumor infiltration by neutrophils and offers an opportunity for neutrophil-mediated drug delivery. We herein showed that the IRE-induced neutrophil trafficking was facilitated by the upregulation of neutrophil chemotaxis and migration as well as the release of several chemoattractants. Doxorubicin-loaded bovine serum albumin nanoparticles were prepared and loaded into neutrophils at a ratio of 9.9 ± 1.2 to 11.7 ± 2.0 pg of doxorubicin per cell. The resultant formulation (NP@NEs) efficiently accumulated in the IRE-treated KPC-A377 murine pancreatic tumors with an uptake value of 10.7 ± 1.5 (percent of injected dose per gram of tissue, abbreviated as %ID/g) at 48 h after intravenous injection. In both Panc02 and KPC-A377 murine pancreatic tumor models, the combination of IRE + NP@NEs inhibited tumor growth more effectively than either monotherapy. The tumors treated with the combination also exhibited the lowest frequency of Ki67+ proliferating cells and the highest abundance of terminal deoxynucleotidyl transferase dUTP nick end labeling+ (TUNEL+) apoptotic cells among the experiment groups. Minimal treatment-associated toxicity was observed. Our findings suggest that neutrophil-mediated delivery of chemotherapy drugs is a useful tool to enhance the response of pancreatic cancer to IRE.

Influence of pump laser fluence on ultrafast myoglobin structural dynamics.

High-intensity femtosecond pulses from an X-ray free-electron laser enable pump-probe experiments for the investigation of electronic and nuclear changes during light-induced reactions. On timescales ranging from femtoseconds to milliseconds and for a variety of biological systems, time-resolved serial femtosecond crystallography (TR-SFX) has provided detailed structural data for light-induced isomerization, breakage or formation of chemical bonds and electron transfer1,2. However, all ultrafast TR-SFX studies to date have employed such high pump laser energies that nominally several photons were absorbed per chromophore3-17. As multiphoton absorption may force the protein response into non-physiological pathways, it is of great concern18,19 whether this experimental approach20 allows valid conclusions to be drawn vis-à-vis biologically relevant single-photon-induced reactions18,19. Here we describe ultrafast pump-probe SFX experiments on the photodissociation of carboxymyoglobin, showing that different pump laser fluences yield markedly different results. In particular, the dynamics of structural changes and observed indicators of the mechanistically important coherent oscillations of the Fe-CO bond distance (predicted by recent quantum wavepacket dynamics21) are seen to depend strongly on pump laser energy, in line with quantum chemical analysis. Our results confirm both the feasibility and necessity of performing ultrafast TR-SFX pump-probe experiments in the linear photoexcitation regime. We consider this to be a starting point for reassessing both the design and theinterpretation of ultrafast TR-SFX pump-probe experiments20 such that mechanistically relevant insight emerges.

Two-Step Relaxation of Non-Equilibrium Electrons in Graphene: The Key to Understanding Pump–Probe Experiments

In the majority of experiments targeting nonlinear optical phenomena, the application of high-intensity pulses drives electrons in graphene into a strongly non-equilibrium state. Under these conditions, conventional perturbation theory falls short in explaining graphene’s intricate optical response because of significant deviations in electron distribution over energy states from the equilibrium Fermi-Dirac one. In this work, we present a two-step relaxation model capable of predicting the transient dynamics of graphene’s carriers out of equilibrium, from the generation of spectrally narrow populations of non-thermalized electrons and holes to the establishment of a hot-electron gas and its subsequent cooling toward equilibrium with the crystal lattice. By comparing our model calculations to experimental results, we demonstrate its reliability and relevance to pump–probe experiments, providing insights into the pivotal role of hot electrons in comprehending ultrafast dynamics in graphene.

Retrospective clinical evaluation of Q-switched Nd:YAG laser safety and efficacy in tattoo removal: A new perspective on the Kirby-Desai scale.

With nearly 50% of the population in the United States, Italy, and Sweden tattooed with at least one tattoo, the demand for its removal has risen by 32% since 2011. Traditional removal methods, such as Q-switched (QS) laser-based tattoo removal, can be lengthy, requiring up to 20 sessions. This study presents a retrospective clinical evaluation of seven short-pulsed QS, dual-wavelength Nd:YAG laser, as an efficient alternative, that can potentially reduce the number of sessions needed as calculated by a founded scale. The QS modality delivers high-intensity pulses in four wavelengths, ideal for removing multicolored tattoos, of which two were used.We studied 11 patients who underwent 3-8 treatments (average 5.09) every 2-3 months. Each tattoo was assessed using the Kirby-Desai scale, considering the following factors: location, Fitzpatrick skin type, ink amount, layering, scarring, tissue changes, and tattoo color. Follow-ups were conducted after 6 months and at 4-5 years following last session. The long-term follow-up presented a significantly higher tattoo removal efficiency than the short-term follow-up (p < 0.001), indicating a sustained process of ink breakdown and elimination. Notably, the actual number of treatments were significantly lower than that predicted by the Kirby-Desai scale (average 5.09 vs. 9.9, p < 0.001). No severe adverse events were reported. In conclusion, the QS Nd:YAG laser offers a safe and effective alternative for tattoo removal, requiring fewer treatments than initially expected.

Quenched lattice fluctuations in optically driven SrTiO3

Crystal lattice fluctuations, which are known to influence phase transitions of quantum materials in equilibrium, are also expected to determine the dynamics of light-induced phase changes. However, they have only rarely been explored in these dynamical settings. Here we study the time evolution of lattice fluctuations in the quantum paraelectric SrTiO3, in which mid-infrared drives have been shown to induce a metastable ferroelectric state. Crucial in these physics is the competition between polar instabilities and antiferrodistortive rotations, which in equilibrium frustrate the formation of long-range ferroelectricity. We make use of high-intensity mid-infrared optical pulses to resonantly drive the Ti–O-stretching mode at 17 THz, and we measure the resulting change in lattice fluctuations using time-resolved X-ray diffuse scattering at a free-electron laser. After a prompt increase, we observe a long-lived quench in R-point antiferrodistortive lattice fluctuations. Their enhancement and reduction are theoretically explained by considering the fourth-order nonlinear phononic interactions to the driven optical phonon and third-order coupling to lattice strain, respectively. These observations provide a number of testable hypotheses for the physics of light-induced ferroelectricity.

Optimal remote restoring of quantum states in communication lines via local magnetic field

Optimal state transport across spin chains, which are proposed as quantum wires for information transfer in solid state quantum architectures, is an important topic for quantum technologies. In this work, we study the remote restoring of a quantum state transferred along a spin chain. The structural state-restoring technique provides proportionality between the appropriate elements of the density matrices of the initial sender state and receiver state at some time instant. We develop a remote state-restoring protocol which uses an inhomogeneous magnetic field with step-wise time-dependent Larmor frequencies as the state-control tool. For simulating the multiparametric Hamiltonian we use two approximating models. First model is based on the Trotter-Suzuki method, while the second model is based on using short pulses of high intensity. In both cases we estimate the accuracy of the approximation and find the optimal restoring parameters (Larmor frequencies) of the protocol which maximize the coefficients in the proportionality for spin chains of various lengths.

Study of population dynamics toward the development of atomic X-ray lasers in the 3–5 keV photon energies

X-ray free-electron lasers (XFELs) are powerful tools for characterizing and probing the properties of matter at atomic resolution on the ultrafast timescale. However, they have certain limitations such as spectral fluctuation and poor temporal coherence. Atomic X-ray lasers offer the narrow bandwidth, longitudinal coherence, and spectral stability that can overcome these limitations. In this paper, we study the interaction of inner-shell vacancy states with high-intensity XFEL pulses. We show that it is possible to achieve population inversion between K-shell and L-shell vacancy states in calcium and titanium when pumped by high-intensity XFEL pulses. These states can be used to generate atomic X-ray laser emission in the 3–5 keV photon energy range.

Direct numerical simulations of long-range infrasound propagation: Implications for source spectra estimation.

The evolution of observed dominant frequencies from a high-intensity infrasonic pulse with receiver range and stratospheric temperature is investigated using direct numerical simulations of the two-dimensional unsteady compressible Navier-Stokes equations. There is a high level of uncertainty in estimating source dominant frequencies based on received signals at sparse points on the ground. Nonlinear propagation effects in the ground-level thermospheric arrivals are found to significantly alter dominant frequency measurements compared to stratospheric arrivals with smaller amplitude sources. With a larger amplitude source, variations in observations are minimized as a result of nonlinear effects being ubiquitous across all atmospheric components of received signals but have a greater offset to the source dominant frequency. An approach to determine the source dominant frequency and minimize atmospheric variability is presented by calculating a source-to-receiver spectral transfer function averaged across the atmospheric states. This method reduces atmospheric variability in source frequency estimates within the pseudo-linear propagation regime and the average error to the known source frequency with a large amplitude source. The reduction of errors in source frequency estimates demonstrates the feasibility of using remote infrasound measurements as an indicator of source frequency and, in turn, the explosive yield of clandestine nuclear weapon test explosions.

Dissociation of HeH+ in the electronic ground state using shaped mid-IR laser pulses.

Inspired by recent experimental work, we study the control over the laser-driven dissociation of the HeH+ ion in the electronic ground state. Shaped pulses with peak intensities below 1012 W cm-2 are obtained by phase modulation of high-intensity transform-limited femtosecond pulses. We investigate the performance of pulse shaping for a number of shaping parameters targeting both vibrational and rotational excitation pathways. The numerical results show that pulse shaping is most effective at low pulse energies and broad spectral bandwidths, while intense transform-limited pulses with narrow spectral bandwidths maximize dissociation. We show that the control achieved with a quadratic chirped pulse optimized for vibrational ladder climbing, a cascade excitation process of adjacent vibrational levels, is hindered by rotational motion leading to significantly reduced dissociation. Moreover, pulse shaping using higher-order polynomial phase functions is found to provide only a marginal increase in dissociation yields. Our results provide additional insights into the coherent control of bond breaking in diatomic molecules, and demonstrate the efficacy of pulse shaping for a range of pulse energies.