High-intensity Exposure Research Articles

Neuromuscular Performance and the Intensity of External Training Load During the Preseason in National Collegiate Athletic Association Division I Men's Collegiate Basketball Players.

Curtis, M, Kupperman, N, Westbrook, J, Weltman, AL, Hart, J, and Hertel, J. Neuromuscular performance and the intensity of external training load during the preseason in National Collegiate Athletic Association Division I men's collegiate basketball players. J Strength Cond Res XX(X): 000-000, 2024-The aim of the study was to determine whether acute changes in neuromuscular performance can be detected through countermovement jumps (CMJs) conducted pre- and postpractice sessions in conditions of high or low intensity measured by microsensors technology. Using an observational repeated measures design, data were collected from 10 male collegiate basketball players. Countermovement jump data were collected before and after practice exposures over 4 weeks of preseason. Select CMJ kinetics were compared in conditions of high and low training load intensity to detect neuromuscular performance changes in displacement of the center of mass and kinetics. Kinetic measures were categorized as output, underpinning, and strategy-related variables. We investigated "output" defined as displacement (jump height [JH]), "underpinning" defined as force-related (mean eccentric force, mean concentric force, force at zero velocity), and "strategy" defined as time-related (countermovement depth [CMD], eccentric duration (EccDur), concentric duration [ConcDur]) variables. There were significant condition × time interactions in CMJ variables namely eccentric mean force (EccForce), force at zero velocity (Force@0), CMDepth, EccDur, and ConcDur. In conditions of high intensity, players had significant, but small decreases in EccForce and Force@0, with small increases in CMD, EccDur, and ConDur, respectively. However, there were no significant decreases in JH. High-intensity practice exposures did not impact neuromuscular performance specific to "output," suggesting that collegiate basketball athletes can maintain JH despite alterations in "underpinning" and "strategy-related" variables. This could have relevance in understanding how fatigue associated with higher-intensity training exposures may potentially alter jump strategy and force production capacities due to external load intensity in collegiate basketball athletes.

MODELING OSTEOCYTE UNDER SHOCK-WAVE THERAPEUTIC LOADING

Dental implants have substantial significance in modern dentistry, but their osseointegration stage remains the most crucial and time-consuming step for compensating for a lost tooth. One promising approach to improve osseointegration rates is the use of extracorporeal shock wave therapy, which has shown efficacy in treating fractures, bone defects, and bone tissue regeneration in surgical and arthroplasty procedures. To comprehend the potential of shock wave therapy in accelerating implant osseointegration, it is crucial to investigate the influence of mechanical loading on ossification processes of varying scales objectively. This study aims to study numerically the effects of low-energy shock wave therapy at different intensities on the mechanical response of a single osteocyte, the principal bone cell that corresponds to microscale of bone tissue. The investigation employs the method of movable cellular automata for modeling. The computer simulation results and analysis based on mechanobiological principles indicate that low-intensity shock wave loading creates conditions for intramembranous ossification, whereas high-intensity shock wave exposure creates conditions for endochondral ossification.

Validation of a novel simulated tendon model for core suture tendon repair.

Simulation training can develop surgical procedural skills in a safe environment. Able to offer high-intensity exposure, simulation is increasingly important as working time for surgeons becomes more protected. Materials used in simulated tendon repair play a critical role in the fidelity and face validity of the model. Although organic materials like porcine tendon are commonly used, non-organic materials offer advantages such as accessibility, reproducibility, cost-effectiveness and ease of use without the need for special licences or facilities. This study aims to establish the face, content and concurrent validity of using a novel silicone material in a simulated tendon repair model. Three tendon models, bathroom silicone sealant, DragonSkin® silicone and organic porcine tendons, were evaluated for concurrent validity through mechanical load to failure testing. Face and content validity were assessed, following participant repair of a DragonSkin® tendon, using a 5-point Likert scale for five clinically relevant parameters. Significant differences in load to failure were observed among bathroom sealant, DragonSkin® and porcine tendon (11.1N, 31.7N and 56.2N; p < 0.001). Participant feedback on the DragonSkin® tendon indicated that it was suitably representative, easy to use and useful for training (agreement rates 58%, 75% and 83%, respectively). However, participants noted that the model did not handle or glide like human tendon (both 8% agreement). DragonSkin® silicone is an adaptable and valid material for simulated tendon repair models. It is low cost, widely available and shows promise as a training tool. Future research will focus on exploring its effectiveness in training settings.

A novel approach to triazole fungicides risk characterization: Bridging human biomonitoring and computational toxicology

Brazil stands as the world's leading coffee producer, where the extensive use of pesticides is economically critical yet poses health and environmental risks due to their non-selective mechanisms of action. Specifically, triazole fungicides are widely used in agriculture to manage fungal diseases and are known to disrupt mammalian CYP450 and liver microsomal enzymes. This research establishes a framework for risk characterization of human exposure to triazole fungicides by internal-dose biomonitoring, biochemical marker measurements, and integration of high-throughput screening (HTS) data via computational toxicology workflows from the Integrated Chemical Environment (ICE). Volunteers from the southern region of Minas Gerais, Brazil, were divided into two groups: farmworkers and spouses occupationally and environmentally exposed to pesticides from rural areas (n = 140) and individuals from the urban area to serve as a comparison group (n = 50). Three triazole fungicides, cyproconazole, epoxiconazole, and triadimenol, were detected in the urine samples of both men and women in the rural group. Androstenedione and testosterone hormones were significantly reduced in the farmworker group (Mann-Whitney test, p < 0.0001). The data show a significant inverse association of testosterone with cholesterol, LDL, VLDL, triglycerides, and glucose and a direct association with HDL (Spearman's correlation, p < 0.05). In the ICE workflow, active in vitro HTS assays were identified for the three measured triazoles and three other active ingredients from the pesticide formulations. The curated HTS data confirm bioactivities predominantly related to steroid hormone metabolism, cellular stress processes, and CYP450 enzymes impacted by fungicide exposure at occupationally and environmentally relevant concentrations based on the in vitro to in vivo extrapolation models. These results characterize the potentially significant human health risk, particularly from the high frequency and intensity of exposure to epoxiconazole. This study showcases the critical role of biomonitoring and utility of computational tools in evaluating pesticide exposure and minimizing the risk.

Light sensitivity in Beroidae ctenophores: Insights from laboratory studies and genomics

Light detection underlies a variety of animal behaviors, including those related to spatial orientation, feeding, avoidance of predators, and reproduction. Ctenophores are likely the oldest animal group in which light sensitivity based on opsins evolved, so they may still have the ancestral molecular mechanisms for photoreception. However, knowledge about ctenophore photosensitivity, associated morphological structures, molecular mechanisms involved, and behavioral reactions is limited and fragmented. We present the initial experiments on the responses of adult Beroe ovata to high-intensity light exposure with different spectra and photosensitivity in various parts of the animal's body. Ctenophores have shown a consistent behavioral response when their aboral organ is exposed to a household-grade laser in the violet spectrum. To investigate the genes responsible for the photosensitivity of Beroidae, we have analyzed transcriptome and genome-wide datasets. We identified three opsins in Beroe that are homologous to those found in Mnemiopsis leidyi (Lobata) and Pleurobrachia bachei (Cydippida). These opsins form clades Ctenopsin1, 2, and 3, respectively. Ctenopsin3 is significantly distinct from other ctenophore opsins and clustered outside the main animal opsin groups. The Ctenopsin1 and Ctenopsin2 groups are sister clusters within the canonical animal opsin tree. These two groups could have originated from gene duplication in the common ancestor of the species we studied and then developed independently in different lineages of Ctenophores. So far, there is no evidence of additional expansion of the opsin family in ctenophore evolution. The involvement of ctenophore opsins in photoreception is discussed by analyzing their protein structures.

Evaluation of Heavy Metal and Specific Trace Elements Levels Among Fast-Food Workers and Their Susceptibility to Atherosclerosis.

Fast-food workers in Iraq face significant health risks due to exposure to heavy metals from fumes and dust during cooking activities. Heavy metals, such as lead (Pb), cadmium (Cd), and nickel (Ni), are toxic to cells even at low concentrations and can cause health risks, including atherosclerosis, due to oxidative stress and reduced antioxidant activity. To the best of our knowledge, this is the first study assess the levels of heavy metals in fast-food workers and investigate their potential link to atherosclerosis development by monitoring the levels of copper (Cu), zinc (Zn), magnesium (Mg), manganese (Mn), and iron (Fe). A total of 120 male participants aged between 20 and 40 years were included in the study, with 40 fast-food workers, 40 patients with atherosclerosis, and 40 healthy individuals evaluated. The levels of Pb, Cd, Ni, Cu, Zn, Mg, Mn, and Fe in all blood samples were determined using atomic absorption spectrometry. Results showed that the fast-food worker group had significantly higher levels of Pb, Cd, Cu, and Fe compared to the healthy control group, with increases of 57%, 75%, 30%, and 55%, respectively. Conversely, their levels of Zn and Mg were significantly lower, decreasing by 15% and 16%, respectively. On the other hand, the atherosclerosis patients' group had significantly higher levels of Pb, Cd, Cu, and Fe, with increases of 47%, 74%, 34%, and 28%, respectively, as well as significantly lower levels of Zn and Mg, decreasing by 17% and 21%, respectively, compared to the control group. These findings suggest that fast-food workers are at risk of developing atherosclerosis due to exposure to high levels of heavy metals and imbalances in essential trace elements. The results showed a significant increase in the levels of Pb and Cd in the sera of these workers, which was expected because of the long duration and high intensity of exposure to toxic heavy metals. This is a serious indicator that must be considered, as it has been previously established that increased levels of Pb and Cd in the body are linked to the risk of atherosclerosis. Additionally, an association between Pb and Cd levels and an imbalance in trace element levels (Cu, Zn, Mg, and Fe) were observed. The Implementation of stricter regulations and guidelines for maintaining cleanliness and safety in fast-food restaurants may be crucial for protecting workers and preventing long-term health complications.

Z-scan of ITO nanocrystals grown inside glass.

Indium tin oxide (ITO) nanocrystals 1-10 nm in size were grown via thermal treatment of a boroaluminosilicate parent glass. The nonlinear behavior of the obtained glass-ceramic was investigated with the Z-scan technique using 550 ps pulses of a 532 nm source at a 500 Hz repetition rate. The nonlinear response was rich, with the sample exhibiting third- and fifth-order nonlinearities as well as saturable absorption and two-photon absorption (TPA), depending on the locale probed. Photoinduced changes were also observed, with high intensity exposures yielding an increased magnitude of the response when lower power trials were subsequently repeated at the same sample position. The work demonstrates that ITO nanocrystal precipitation in bulk glass yields effective nonlinear response and suggests that with further development may enable more compact devices exploiting ITO and the need for particle deposition routes.

Increased vascular stiffness in children exposed in utero but not children exposed postnatally to emissions from a coal mine fire.

Chronic, low-intensity air pollution exposure has been consistently associated with increased atherosclerosis in adults. However, there was limited research regarding the implications of acute, high-intensity air pollution exposure during childhood. We aimed to determine whether there were any associations between early-life exposure to such an episode and early-life vascular function changes. We conducted a prospective cohort study of children (<9 years old) who lived in the vicinity of the Hazelwood coal mine fire (n = 206). Vascular function was measured using noninvasive diagnostic methods including carotid intima-media thickness and pulse wave velocity (PWV). Exposure estimates were calculated from prognostic models and location diaries during the exposure period completed by each participant's parent. Linear mixed-effects models were used to determine whether there were any associations between exposure and changes in vascular outcomes at the 3- and 7-year follow-ups and over time. At the 7-year follow-up, each 10 μg/m3 increase in daily PM2.5 in utero was associated with increased PWV (β = 0.13 m/s; 95% confidence interval [CI] = 0.02, 0.24; P = 0.02). The association between in utero exposure to daily PM2.5 was not altered by adjustment for covariates, body mass index, and maternal fire stress. Each 1 µg/m3 increase in background PM2.5 was associated with increased PWV (β = 0.68 m/s; 95% CI = 0.10, 1.26; P = 0.025), in children from the in utero exposure group. There was a trend toward smaller PWV (β = -0.17 m/s; 95% CI = -0.366, 0.02) from the 3- to 7-year follow-up clinic suggesting that the deficits observed previously in children exposed postnatally did not persist. There was a moderate improvement in vascular stiffness of children exposed to PM2.5 from a local coal mine fire in infancy. There was a mild increase in vascular stiffness in children exposed to PM2.5 from a local coal mine fire while their mothers were pregnant.

Investigating the stretch-shortening cycle fatigue response to a high-intensity stressful phase of training in collegiate men's basketball.

While using force-plate derived measures of vertical jump performance, reflective of stretch-shortening-cycle (SSC) efficiency is common practice in sport science, there is limited evidence as to which tests and measures may be most sensitive toward neuromuscular fatigue. The aim of this study was to explore the SSC fatigue response to a one-week high-intensity fatiguing phase of training in National Collegiate Athletic Association (NCAA) Division-I basketball players. The study timeline consisted of three weeks of baseline measures, one week of high-intensity training, and two weeks of follow-up testing. Countermovement jumps (CMJ) and 10-5 hop tests were performed at baseline, as well as at two time-points during, and three time-points following the fatiguing training period, allowing for performance-comparisons with baseline. Compared to the weekly training sum at baseline, during the high intensity training phase, athletes were exposed to very large increases in selected external load metrics (ES = 1.44-3.16), suggesting that athletes experienced fatigue acutely, as well as potential longer lasting reductions in performance. Vertical jump data suggested that in the CMJ, traditional metrics such as jump height, as well as metrics reflecting kinetic outputs and movement strategies, were sensitive to the stark increase in high-intensity training exposure. The 10-5 hop test suggested a fatigue-induced loss of tolerance to ground impact reflected by performance reductions in metrics related to jump height and reactive strength qualities. These findings emphasize that when monitoring neuromuscular fatigue, variables and assessments may not be looked at individually, but rather as part of a more global monitoring approach.

Temporal Alterations in Cerebrovascular Glycocalyx and Cerebral Blood Flow after Exposure to a High-Intensity Blast in Rats.

The glycocalyx is a proteoglycan-glycoprotein structure lining the luminal surface of the vascular endothelium and is susceptible to damage due to blast overpressure (BOP) exposure. The glycocalyx is essential in maintaining the structural and functional integrity of the vasculature and regulation of cerebral blood flow (CBF). Assessment of alterations in the density of the glycocalyx; its components (heparan sulphate proteoglycan (HSPG/syndecan-2), heparan sulphate (HS), and chondroitin sulphate (CS)); CBF; and the effect of hypercapnia on CBF was conducted at 2-3 h, 1, 3, 14, and 28 days after a high-intensity (18.9 PSI/131 kPa peak pressure, 10.95 ms duration, and 70.26 PSI·ms/484.42 kPa·ms impulse) BOP exposure in rats. A significant reduction in the density of the glycocalyx was observed 2-3 h, 1-, and 3 days after the blast exposure. The glycocalyx recovered by 28 days after exposure and was associated with an increase in HS (14 and 28 days) and in HSPG/syndecan-2 and CS (28 days) in the frontal cortex. In separate experiments, we observed significant decreases in CBF and a diminished response to hypercapnia at all time points with some recovery at 3 days. Given the role of the glycocalyx in regulating physiological function of the cerebral vasculature, damage to the glycocalyx after BOP exposure may result in the onset of pathogenesis and progression of cerebrovascular dysfunction leading to neuropathology.

Demographic disparity in diurnal surface urban Heat Island exposure across local climate zones: A case study of Chongqing, China

Surface urban heat island (SUHI) exposure significantly harms human health during rapid urbanization. Identifying the areas and demographic groups under high SUHI exposure is critical for mitigating heat-related hazards. However, despite broad concern in US-European countries, rare studies discuss the diurnal SUHI exposure of demographic subgroups across Local Climate Zones (LCZs) in Chinese cities. Therefore, taking Chongqing as the case study, we measured the diurnal SUHI exposure of demographic subgroups (e.g., gender, age, and income) across different LCZs (compact, open, and sparsely-built zones) by coupling the ECOSTRESS data and mobile phone signaling data. The results indicated that Chongqing's compact high/middle-rise zones suffered a higher SUHI exposure due to high land surface temperature (LST) and a larger size of population than open zones. Despite a relatively low population density, extremely high LST in compact low-rise zones (e.g., industrial parks) contributes to considerable accumulated SUHI exposure. The SUHI exposure risk exhibited the differences between daytime and nighttime, resulting from SUHI variation and population flow. The demographic analysis showed that Chongqing's demographic subgroups are exposed disproportionately to SUHI. Elderly groups suffered relatively high exposure in compact high-rise zones. Low-incomers witnessed a high exposure in open zones. These findings call for alleviating SUHI exposure risk by targeting vulnerable groups and high-intensity exposure areas.

Exploring influential factors and strategies for addressing speech delay of a child with autism spectrum disorder (ASD) in English (L2) language acquisition

Children with autism spectrum disorder (ASD) tend to exhibit a unique trajectory in the acquisition of language with some documented research. However, research investigating children with ASD in English (L2) language acquisition is limited, particularly in Indonesian ASD children. This research explores the factors that allowed this unanticipated foreign language acquisition, thereby causing delays in native speech, and investigates strategies that could help overcome the child's linguistic challenges. This case study research involved a six-year-old Indonesian ASD child experiencing early self-learning of English and speech delay in her native language. The data were garnered through observations, interviews, and field notes before being thematically analyzed. The results show that the internal factor of speech delay is prematurity, and the external influential factors are the high intensity of English language exposure through media, English daily interactions with family members, and the use of different first languages in the social environment. Successful strategies employed to acquire the child’s native language include the habituation of speaking Indonesian, the repetition method, and the implementation of a language teaching model by teachers at school. This study underscores the importance of the social environment to promote language acquisition and the possible interventions to cope with speech delay for ASD children.

Guarding the gatekeepers: a comprehensive approach to control nosocomial measles.

Despite substantial vaccination progress, persistent measles outbreaks challenge global elimination efforts, particularly within healthcare settings. In this paper, we critically review the factors contributing to measles outbreak and effective control measures for nosocomial transmission of measles. We systematically searched electronic databases for articles up to 17th May, 2023. This was performed by two independent reviewers, with any disagreements resolved by a third reviewer. We also searched governmental and international health agencies for relevant studies. Forty relevant articles were systematically reviewed, revealing key factors fuelling measles outbreak in healthcare settings, including high transmissibility capability; high intensity exposure; delayed care; failure to use protective equipment and implement control measures; vaccine failure; unclear immunisation history and lack of registries; and lacking recommendation on healthcare workers' (HCWs) measles vaccination. To combat these challenges, successful control strategies were identified which include early notification of outbreak and contact tracing; triaging all cases and setting up dedicated isolation unit; strengthening protective equipment use and physical measures; improving case detection; determining immunity status of HCWs; establishing policy for measles vaccination for HCWs; management of exposed personnel; and developing a pre-incident response plan. A coordinated and comprehensive approach is essential to promptly identify and manage measles cases within healthcare settings, necessitating multifactorial strategies tailored to individual settings. These findings provide a valuable foundation for refining strategies to achieve and maintain measles elimination status in healthcare environments.

Effect of cryptochrome 1 deficiency and spectral composition of light on photosynthetic processes in A. thaliana under high-intensity light exposure

Effect of cryptochrome 1 deficiency and spectral composition of light on photosynthetic processes in A. thaliana under high-intensity light exposure

Flow velocity and light intensity combination is important for Microcystis aeruginosa physical suppression.

The cyanobacterial response to flow velocity or light intensity deviates from the combined effect of both factors. The responses of Microcystis aeruginosa to different combinations of flow velocities and light intensities were tested. Growth (OD730 and protein), stress (catalase, ascorbate peroxidase, and glutathione peroxidase), and photosynthetic ability (chlorophyll-a and fluorescence) parameters of M.aeruginosa were measured to evaluate the effects of different combinations. Exposure to different flow velocity-light combinations significantly affected the growth and physiology of M.aeruginosa. Flow velocities of 0.4m s-1 showed a prominent influence on most of the measured parameters compared with no flow velocity or higher flow velocity conditions. The 1.2-m s-1 flow velocity and high light intensity (1200 μmol m-2 s-1 ) exposure caused a significant elevation in oxidative stress. Lower velocities are beneficial for M.aeruginosa at light stress, whereas extreme velocities are adverse and elevate the stress. Two categories of light-velocity combinations were identified as preferred and extreme categories, depending on whether they suppressed or supported M.aeruginosa growth. In controlling cyanobacteria blooms using flow or high-intensity light, it is imperative to consider the interaction of these two factors, as their combined effects can significantly vary the stress levels in cyanobacteria. A new system, designed to minimize mechanical damage on M.aeruginosa, was used to generate flow velocities. Additionally, the combined effects of flow velocities and light intensities have been considered for the first time. PRACTITIONER POINTS: Flow velocity can influence the effect of light on Microcystis aeruginosa. High light exposure effect on Microcystis aeruginosa can be reduced by low flow velocity. High flow velocity and high light exposure increase the stress on Microcystis aeruginosa. Different light intensities and flow velocity combinations changed Microcystis aeruginosa stress physiology.

Lung function changes in children exposed to mine fire smoke in infancy.

Chronic, low-intensity air pollution exposure has been consistently associated with reduced lung function throughout childhood. However, there is limited research regarding the implications of acute, high-intensity air pollution exposure. We aimed to determine whether there were any associations between early life exposure to such an episode and lung growth trajectories. We conducted a prospective cohort study of children who lived in the vicinity of the Hazelwood coalmine fire. Lung function was measured using respiratory oscillometry. Z-scores were calculated for resistance (R5 ) and reactance at 5 Hz (X5 ) and area under the reactance curve (AX). Two sets of analyses were conducted: (i) linear regression to assess the cross-sectional relationship between post-natal exposure to mine fire-related particulate matter with an aerodynamic diameter of less than 2.5 micrometres (PM2.5 ) and lung function at the 7-year follow-up and (ii) linear mixed-effects models to determine whether there was any association between exposure and changes in lung function between the 3- and 7-year follow-ups. There were no associations between mine fire-related PM2.5 and any of the lung function measures, 7-years later. There were moderate improvements in X5 (β: -0.37 [-0.64, -0.10] p = 0.009) and AX (β: -0.40 [-0.72, -0.08] p = 0.014), between the 3- and 7-year follow-ups that were associated with mean PM2.5 , in the unadjusted and covariance-adjusted models. Similar trends were observed with maximum PM2.5 . There was a moderate improvement in lung stiffness of children exposed to PM2.5 from a local coalmine fire in infancy, consistent with an early deficit in lung function at 3-years after the fire that had resolved by 7-years.

Justification of obtaining fine-grained structure of welded joints at high-intensity impulse effect on welding circuit

An effective method of improving the reliability of operation of thermal and nuclear power facilities is to improve the quality of manufacture, installation and repair of their thermal and generating power equipment. One of the ways to improve the quality, technological and service properties of welded joints in the process of their implementation is to influence the structure of the crystallizing metal by thermal, electric high-intensity impulse effect for its grinding. This work proposes the results of an experimental study to substantiate the production of a fine-grained structure of welded joints obtained using manual arc welding with coated electrodes at a high-intensity impulse effect (QPS) with a fi.g.= 40×103 Gts frequency, voltage Ui.g.= 80.0 V, on the welding circuit. The energy characteristics of the process can be used to assess the effect of high-intensity impulse action on the welding circuit, including the arc plasma and the structure of the resulting weld. As the energy characteristics of the welding process, the welding current Iwd, the voltage on the arc discharge Ud, the power Rp. Oscillograms of the specified characteristics were obtained, as well as the values of the maximum (peak) and average power released in the welding circuit when QPS is exposed to it and without its use were determined. Energy evaluation of input of additional high-intensity pulse effect on welding circuit as ultrasonic energy for cavitations of surface layer of welding bath at QPS was performed. Direct current arc discharge at application of high-intensity pulse effect with frequency of fi.g.= 40×103Gts (QPS) is source of cavitations of liquid phase of metal of welding bath in limited surface layer of preset thickness. It can be assumed that the crystallization of the bath takes place in layers when the welding circuit is subjected to high-intensity pulse exposure with a frequency of fi.g.= 40×103 Gts (QPS). In this case, the growing crystals break when the liquid phase oscillates due to friction forces arising between the moving liquid phase and the growing crystal. At the site of crystal fracture, zones of dynamically super cooled metal are formed, which leads to the appearance of new crystallization centers, and a fine-grained structure of the weld appears.

Effect of high-intensity ultrasonic field on fog droplet coagulation efficiency

The relevance. The urgent need to deal with fog for preventing limited visibility and creating improved meteorological conditions. Exposure to gas dispersed systems by mechanical vibrations of the ultrasonic frequency can be considered as the most effective way to deal with fog formation. However, the acoustic method has not found an industrial application for fog destruction mainly due to the lack of effective sources of acoustic exposure. Therefore, it is necessary to create emitters of higher power that provide a significantly greater range of radiation with a sound pressure level of at least 140 dB and study of their functionality. The main aim. Determination of the effectiveness of fog destruction through the use of ultrasonic vibrations generated by the developed ultrasonic emitters; identification of the most promising design of ultrasonic equipment that ensures effective destruction of fogs in open areas. Objects. Combining fog droplets under high-intensity ultrasonic exposure using specially designed ultrasonic emitters for gas media of four types. Methods. Experimental method for studying fog droplets combination under the influence of ultrasonic vibrations and their gravitational deposition. To determine the aerosol (water content and disperse composition) characteristics, during experimental studies, the authors have used the TIPAS-1 meter based on the method of small-angle scattering and the method of spectral transparency. Results. The authors established and shown the effectiveness of ultrasonic effect on fogs, as well as the possibility of its use for fog destruction in an open area. All developed disc ultrasonic emitters are capable of forming a sound pressure level within 140... 145 dB at 22±2.0 kHz. The time of fog natural destruction in the aerosol chamber is reduced by up to 11.5 times. The results obtained demonstrate that it is necessary to use more powerful disc emitters, since they can significantly reduce the time of fog destruction and increase the volumes voiced at the same time. The minimum time for establishing the required metrological range of visibility is provided by means of a directional emitter with a stepped-variable surface, forming a flat wave.

Effects of light exposure intensity and time on printing quality and compressive strength of β-TCP scaffolds fabricated with digital light processing

Digital light processing (DLP), derived from stereolithography (SL), is a lithography-based additive manufacturing (AM) process that offers the ability to tailor scaffold shape, size, material, and biometrics of fabricated scaffolds to patient requirements. The additive manufacturing of tricalcium phosphate (TCP) bioceramic scaffolds via DLP is explored in the proceeding research paper. Particularly, the role of exposure intensity and exposure time on resultant scaffold quality and mechanical properties is studied. Increased exposure intensity results in an increased deviation from the designed lattice structure and thus an increase in compressive strength. Scaffolds produced with the highest exposure intensity, resulted in a 27.6 % error increase in mean beam diameter, resulting from an increased scattering effect, penetration depth, and over-curing of peripheral slurry. TCP scaffolds with an exposure time of 3.5 s and exposure intensity of 40 mW/cm2 possessed the highest print accuracy, resulting in the lowest average compressive strength – 82 MPa for the scaffolds of cube structure and 48 MPa for the ones of I-WP structure. Statement of significanceDigital light processing (DLP) is an effective additive manufacturing (AM) method applicable for bioceramics. This study focuses on the influence of light exposure intensity on scaffold quality and compressive strength. The increase of exposure intensity can improve the compressive strength with the sacrifice of structure accuracy. The conclusions of this study can provide insights into the relationship of DLP printing parameters and scaffolds quality.

HIGH INTRAOCULAR PRESSURE-INDUCED RETINAL ISCHEMIA AND LIGHT-INDUCED RETINOPATHY IN RAT MODELS

Glaucoma and retinal degenerations are two important ocular diseases that often cause massive impacts to vision in both humans and animals. Rat models are commonly used to explore the complex pathophysiology and potential treatments of these diseases. The models of high intraocular pressure (HIOP)-induced retinal ischemia-reperfusion imply the ischemic outcome weight on the inner retina layers (including the nerve fiber layer, retinal ganglion cell (RGC) layer, inner plexiform layer (IPL), and inner retinal layer (INL)) that eventually progressed to the outer retinal layers. Depending on the duration (cycles) of ischemic treatment, more glaucoma pathological change signs may be exhibited more obviously with time. The model requires a short ischemic treatment and anticipates an adequately long period of disease manifestation. To investigate photoreceptor-led retinal degeneration, rat models for light-induced retinopathy are commonly used and it is predominantly attributed to the photoreceptor cells damage of ONL and OPL loss by high intensity of light exposure. This model unraveled the pathophysiological impairment of phototransduction as well as disease mechanisms involving oxidative stress and inflammatory process of the outer retinal layer. With the knowledge gained from the research using these animal models, better understanding of the disease mechanisms in terms of its pathophysiology and molecular changes can be achieved. Besides, the rat models can serve as the key basis for further investigation into the therapeutic or preventive perspectives of these retinopathies.