1. 1. A monochromator has been developed for use with a high intensity lamp by means of which leaves can be illuminated throughout the visible spectrum with light monochromatic within 5 mμ. The beam is of such intensity that the protochlorophyll-chlorophyll transformation produced by periods of illumination of from 20 sec. to 6 min. is sufficient for accurate measurement. 2. 2. Methods of analysis have been worked out whereby the percentage transformation of protochlorophyll can be determined with a precision of about 2%. 3. 3. An albino mutant of corn has become available which when grown in the dark contains approximately the normal amount of protochlorophyll, and upon illumination transforms it into chlorophyll. The action spectrum for the transformation has been measured in this albino strain and in the normal strain from which it segregates. 4. 4. The two corn strains and also bean leaves show a prominent peak in the action spectrum at 650 mμ. At this wavelength 20% of the protochlorophyll was transformed to chlorophyll in 18.4 sec. by an intensity of 79.8 ergs/cm. 2/sec. 5. 5. Another prominent peak lies at 445 mμ. In normal corn and bean leaves, this peak is considerably lower than the peak at 650 mμ, the ratio being 0.66. In contrast, the albino corn uses blue light even more effectively than it does red, the ratio of the peaks at 445 and 650 mμ being 1.89. 6. 6. Since the normal corn leaves contain abundant carotenoids and the albino does not, the lower efficiency of normal etiolated leaves in the blue can be attributed to competitive absorption of light by carotenoid pigments. 7. 7. If some chlorophyll is formed by a pre-exposure to light and the leaves are then further illuminated at 680 mμ (light which is strongly absorbed by chlorophyll but only weakly by protochlorophyll) only a little additional chlorophyll is produced. The increase in chlorophyll can be attributed to the slight absorption by protochlorophyll. 8. 8. Because of the sensitivity and precision of the methods used, the action spectrum for the conversion of protochlorophyll in the leaf is known with an accuracy approaching that of the absorption spectrum of extracted protochlorophyll. The two spectra are similar and the positions of the spectra are in agreement if allowance is made for the shift due to the influence of solvent.
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