Higher Hematocrit Research Articles

Assessment of Donkey (Equus asinus africanus) Whole Blood Stored in CPDA-1 and CPD/SAG-M Blood Bags.

Simple SummaryThe development of conservative solutions was essential to store blood for different periods, but there is no blood bag available for animals. Currently, the solution containing citrate, phosphate dextrose, and adenine (CPDA-1) and the solution containing citrate, phosphate, and dextrose plus mannitol and sodium chloride (CPD/SAG-M) are the most used in human blood conservation. In this study, we propose to evaluate whether the CPDA-1 and CPD/SAG-M blood bags designed for humans are efficient for the conservation of donkey whole blood for 42 days. During storage, both blood bags resulted in mild alterations in the stored blood, but the two bags were efficient and very similar in preserving donkey blood for up to 42 days. Both types of human-designed blood bags can be used for donkey transfusion medicine.Hemotherapy using whole blood and its components is being increasingly used in veterinary therapy. Since it is important to store animal blood while maintaining acceptable hematological, blood gas, and biochemical characteristics, increasing our knowledge of available technologies for strategic blood storage is imperative. Thus, we aimed to assess the hematological, blood gas, and biochemical changes in donkey whole blood using blood bags with two different types of storage agents. Eight adult healthy male donkeys were used; 900 mL of blood was collected from each, with 450 mL stored in citrate-phosphate-dextrose and adenine bags (CPDA-1) and 450 mL stored in bags containing citrate-phosphate-dextrose, adenine, mannitol, and sodium chloride (CPD/SAG-M). Both bags were kept refrigerated between 1 and 6 °C for 42 days. Blood samples were removed from the bags eight times (T): T0 (immediately after blood collection), T1, T3, T7, T14, T21, T35, and T42 (1, 3, 7, 14, 21, 35 and 42 days after storage). Hematological, blood gas, biochemical, and microbiological parameters were assessed. The CPDA-1 bags had a higher packed cell volume when compared to CPD/ SAG-M. The red blood cell count reduced by around 19% in both the bags due to hemolysis, which was confirmed by an increase in plasma hemoglobin. The white blood cell count; pH; concentrations of glucose, sodium, bicarbonate, and 2,3 diphosphoglycerate were reduced in both bags. Meanwhile, pO2, pCO2, lactate dehydrogenase, and levels of potassium increased in the CPDA-1 and CPD/SAG-M bags. Blood bags were efficient for the storage of donkey blood for up to 42 days.

Evaluation of protection in grazing lambs immunised with different doses of Haemonchus contortus gut membrane glycoproteins in Southern Brazil

A vaccine containing doses ranging from 2 μg to 50 μg of integral membrane proteins from Haemonchus contortus intestinal cells (H11 and H-gal-GP complex) has been shown to be effective for lambs. A vaccine for H. contortus was tested in two-month old grazing Corriedale lambs during an eight-month trial on the outskirts of Bagé, Rio Grande do Sul, Brazil. The animals were kept in a single paddock and allocated to five similar groups according to weight, sex and faecal egg counts (FEC). Vaccinated lambs received 0, 2, 5, 10 or 50 μg of the same antigen diluted in QuilA adjuvant. Vaccine injections were given at days 0, 21, 49, 91, 133, and 175. Lambs were sampled weekly for FEC, packed cell volume (PCV), and plasma ELISA antibody titre. Lambs with PCVs ≤15 % were drenched with levamisole (7.5 mg/kg body weight) as a salvage treatment. During days 77–98 an artificial challenge was administered to increase parasite transmission. Post-artificial challenge (from day 98–217), the FEC of the vaccinated lambs were 59.3 % lower than those of the control lambs. Antigen dose correlated with changes in PCV (r = 0.387 p-value < 0.001). Vaccinated lambs also had higher PCV than controls (p < 0.001; contrast analysis). Salvage treatment was needed in 16.7 % of vaccinated lambs and 88 % of control lambs.

Effects of timing of umbilical cord clamping on preventing early infancy anemia in low-risk Japanese term infants with planned breastfeeding: a randomized controlled trial

BackgroundJapanese infants have relatively higher risk of anemia and neonatal jaundice. This study aimed to assess the effects of delayed cord clamping (DCC) on the incidence of anemia during early infancy in low-risk Japanese term infants with planned exclusive breastfeeding for 4 months. This study also aimed to explore the effects of DCC on neonatal jaundice.MethodsWe conducted an open-label, parallel-arm, multicenter randomized controlled trial of DCC (clamping the cord after more than a minute or pulsation stops) vs. early cord clamping (ECC; clamping the cord within 15 s) at one birth center and two clinics in Japan. Low-risk pregnant women planning to have a vaginal birth and to exclusively breastfeed and term singleton infants delivered in cephalic presentation were included in this study. The primary outcome was spectrophotometric estimation of hemoglobin at 4 months. Secondary outcomes were anemia incidence at 4 months, four outcomes related to neonatal jaundice, hematocrit levels, and related outcomes.ResultsOverall, 150 pregnant women were recruited. Participants (N = 138) were randomly allocated to two groups (DCC n = 68, ECC n = 70). There were no significant differences between the two groups in spectrophotometric estimation of hemoglobin at 4 months: mean difference = 0.1 g/dL, 95% confidence interval − 0.14, 0.35, DCC 12.4 g/dL, ECC 12.3 g/dL. Only the hematocrit levels on days 3 to 5 were significantly higher in the DCC group than in the ECC group: DCC 57.0%, ECC 52.6%, mean difference = 4.4, 95% confidence interval 2.61, 6.20. There were no significant differences in other secondary outcomes, including outcomes related to neonatal jaundice.ConclusionAmong low-risk Japanese term infants with planned exclusive breastfeeding, DCC showed no significant effects on spectrophotometric hemoglobin levels at 4 months compared with ECC. We observed significantly higher hematocrit levels on days 3 to 5 in infants who underwent DCC, while these levels were within the normal range. Jaundice outcomes remained similar to those of infants who underwent ECC. Although a larger sample size is required to assess the effects of cord clamping on neonatal jaundice, DCC may prevent anemia in newborn infants.Trial registrationUMIN-CTR; UMIN000022573, 06/01/2016 - retrospectively registered, https://upload.umin.ac.jp/cgi-open-bin/ctr/ctr_view.cgi?recptno=R000023056

COMPARING THE EFFECTS OF LITHIUM HEPARIN AND DIPOTASSIUM ETHYLENEDIAMINETETRAACETIC ACID ON HEMATOLOGIC VALUES IN EASTERN BOX TURTLES (TERRAPENE CAROLINA CAROLINA).

Anticoagulants are employed to prevent clotting and preserve cellular morphology for clinical pathology tests. Lithium heparin (LH) is the most frequently used anticoagulant in chelonians; however, dipotassium ethylenediaminetetraacetic acid (EDTA) may be superior in some species. Although eastern box turtles' (Terrapene carolina carolina) hematologic parameters are well studied, the effects of different anticoagulants on hematology in this species are unknown. This study evaluated the effects of LH and EDTA on hematologic values in free-living eastern box turtles (N = 59). Blood samples were collected from eastern box turtles in Illinois and immediately divided between LH and EDTA microtainers, and complete blood counts were performed on each sample. Grossly, plasma from EDTA blood samples was frequently and significantly hemolyzed. Blood mixed with LH had higher packed cell volume (PCV) (P = 0.04), white blood cell count (WBC) determined by Leukopet (P < 0.0001), WBC determined by blood film estimate (P < 0.0001), absolute heterophils (P = 0.007), absolute lymphocytes (P < 0.0001), and lower total solids (P < 0.0001) and absolute monocytes (P = 0.0001) than blood mixed with EDTA. All relative leukocyte counts were significantly different between the anticoagulants (P < 0.0001). EDTA apparently lysed turtle erythrocytes in this study, making it difficult to accurately count white blood cells and artificially lowering PCV. These findings demonstrate that EDTA should not be used in eastern box turtles.

Evaluation of Haematological and Biochemical Changes in Catfish (Clarias Gariepinus) Fed Ginger Additive Meal Diets

Clarias gariepinus adults were fed varying levels of ginger additive meal diets (0 mg/g, 25 mg/g, 50 mg/g, 75 mg/g and 100 mg/g), respectively, in order to evaluate their effects on the biochemical and haematological parameters of fish. Blood biochemical analysis was performed at the end of 84 days by taking the portion of the collected blood and was dispensed into non-heparinised tubes. There were significant increases (P &lt; 0.05) in glucose, aspertate transferase, alanine transferase, and sodium concentrations respectively. The activity of potassium, creatinine and serum protein changed in a non-significant (P &gt; 0.05) manner with increasing concentrations of additive diets compared to the control. Although no definite trend in the values of computed biochemical indices was observed. However, glucose, aspertate transferase (AST), alanine transferase (ALT), urea, creatinine, sodium, and potassium increased with varying additive inclusion concentration. Also, Glucose, ALT, AST, Urea, creatinine, sodium and potassium activities of all the fish, which have been fed ginger additive meal diets were significantly higher (p&lt;0.05) in comparison to their respective control. Results also showed that there were increase in the blood parameters of Clarias gariepinus fed ginger additive meal diets compared to the control diet except in mean cell haematocrit (MCV) but were statistically different (p&lt;0.05). Highest Packed cell volume (PCV) of 31.00% were recorded in diet (GD3), White Blood Cell (WBC), 8.00 x 103 in GD2, and red blood cell (RBC) 8.70 x 106; while highest Haemoglobin (Hb), 11.00 g/dl were recorded in fish fed diet GD3. All blood parameters obtained were between the range of recommended fish blood. It is concluded that using ginger as additive feed on Clarias gariepinus showed a slight increase in the haematological parameters compared with the control diet (GD1) except in MCV but it has no negative effect on the health status of the studied fish.

Physiological evaluation of broiler finishers fed fermented bovine blood and rumen digesta meal

A 35-day feeding trial involving three hundred (300) five (5) week old broilers was carried out in a completely randomized design to evaluate the haematology, serum biochemical and gastro-intestinal characteristics of broiler finishers fed diets containing a mixture of fermented bovine blood and rumen digesta (FBBRD) meal at dietary levels of 0, 5, 10, 15 and 20 %, respectively. The birds were randomly grouped into five, with sixty birds each and four replicates of fifteen birds per treatment. At the end of the experiment, blood samples were collected from eight birds randomly selected from each treatment for haematological and biochemical analyses. The group on 10 % FBBRD diet had significantly (P&lt;0.05) higher packed cell volume, haemoglobin concentration and red blood cells. Eosinophil values of birds on all the treatment groups except those on 5 % FBBRD dietary level were within the normal range. Serum biochemical analysis of the experimental birds recorded significant (P&lt;0.05) differences in total protein, albumin, creatinine, cholesterol, except blood sugar concentrations. Serum concentrations of sodium and potassium of the experimental birds were also significantly (P&lt;0.05) different. This study suggested that FBBRD meal when properly processed and at 10 % dietary level of inclusion, can be used in broiler finisher production without adverse effects on the physiological indices of birds

Dietary supplementation of Vitamin E and selenium on performance and oxidative stability of meat of broiler chickens in a hot climate

Abstract With the increase in consumers’ concern for safe food, it is highly imperative for livestock farmers to adopt feeding practices that enhance good health and high-quality products. A 49-day trial was conducted to ascertain effect of vitamin E and selenium (VE + Se) on performance, haematological indices and oxidative stability of chicken meat. A total of 150 Ross 7-day-old chicks were weighed and allotted to five treatments comprising dietary levels of 0 mg VE + 0 mg SE (Control), 100 mg VE + 0.05 mg Se, 200 mg VE + 0.1 mg Se, 300 mg VE + 0.15 mg Se and 400 mg VE + 0.2 mg Se per kg of feed in a completely randomised design. Feed and water were provided ad libitum. The data collected on performance, haematological indices and oxidative stability of meat were subjected to a one-way analysis of variance in a completely randomised design. Performance indices were significantly (p &lt; 0.05) influenced by VE + Se supplementation. Mean daily live-weight gain (48.68 g /bird /day) was highest (p &lt; 0.05) in the 400 mg VE + 0.2 mg group. The mean daily feed intake (g /bird / day) was highest in birds fed the diet containing 200 mg VE + 0.1 mg. The least or best feed conversion ratio (FCR) was observed in the group fed 400 mg VE + 0.2 mg SE. Glutathione peroxidase (GSH-Px) increased as the level of VE + Se increased with the highest activity in 400 mg VE + 0.2 mg Se group. The highest packed cell volume, haemoglobin and red blood cell values were observed in birds fed the diet containing 400 mg VE + 0.20 mg Se. In conclusion, to ensure good performance of the chickens and improved oxidative stability of chicken meat in hot climate, feeding broilers VE + SE at 400 mg VE + 0.2 mg Se is recommended.

Clinical Value of Low Molecular Weight Heparin in Treatment of Pulmonary Heart Disease Complicated With Respiratory Failure

It is aimed to investigate the clinical value of low-molecular-weight heparin in treatment of pulmonary heart disease complicated with respiratory failure. Between January 2018 and April 2019, we included 120 pulmonary heart disease patients complicated with respiratory failure in this study and divided them into two groups using the double-blind, random method, i.e. the control group and the observation group, with 60 patients in each group. After admission, patients in two groups undertook the regular treatment, while those in the observation group additionally took low molecular weight heparin at 5000 U via subcutaneous injection, twice per d. Before and after treatment, arterial blood-gas indicators [partial pressure of carbon dioxide, partial pressure of oxygen and pH value] and the indicators of hemorheology (high blood viscosity, hematocrit and fibrinogen) and clinical efficacy were evaluated for patients in two groups. Prior to the treatment, comparison of the levels of partial pressure of carbon dioxide, partial pressure of oxygen, pH value, high blood viscosity, hematocrit and fibrinogen between two groups showed no significant differences, while after treatment, significant improvement was demonstrated in these indicators in the observation group as compared to those in the control group (p 0.05). In the observation group, patients had an effectiveness rate of 88.33 % (53/60), significantly higher than 63.33 % (38/60) in the control group (p 0.05), while patients in the observation group had shorter hospitalization time [(14.7±0.4) d] than that [(16.9±0.3) d] in the control group (p<0.05). For pulmonary heart disease patients complicated with respiratory failure, medication of low-molecular-weight heparin gained promising efficacy, with significant improvement in the blood performance, ventilation, and heart and lung functions of patients, thereby increasing the survival rate and shortening the hospitalization time of patients. Thus, it is worthy of being promoted in clinical practice.

Doğuştan Kalp Hastalıklı Çocuklarda Optik Koherens Tomografi ile Koroid Kalınlığının Değerlendirilmesi

Objective: The main reason for complications in congenital heart diseases (CHD) is decreased blood oxygen saturation and polycythemia which are typical for cyanosis. These parameters may promote the damage of the retina because haemodynamic regulation is essential for the structural and functional integrity of the macular subfields. The aim of this study was to evaluate choroidal thickness (CT) measurements in children with CHD using spectral domain optical coherence tomography (OCT). Methods: This prospective study compared 30 CHD and 30 healthy control children. CT was examined with spectralis spectral-domain OCT (Retinascan RS-3000; Nidek). CT was obtained at the subfovea, 500 μm and 1000 μm nasal to the fovea (N500, N1000) and 500 μm and 1000 μm temporal to the fovea (T500, T1000). Only the right eye values were used for statistical comparisons between the groups. The domain cardiac lesions were divided physiologically into two categories: volume overload and cyanotic. Results: Mean age was 11.0±3.5 years in CHD childrens and 10.9±3.6 years in the control group (p=0.971). Children with CHD had no statistically significant CT measurements compared with healthy controls (p&gt;0.05). Conclusion: Although in high haematocrit, low oxygen saturation or the presence of the volume overload in the history of CHD patients, our data suggests that patients with CHD show normal CT. The reason may be medical and surgical treatment of hypoxia, erythrocytosis and volume overload in CHD patients.

Evaluation of quality matrix when practice changed from triple bags to quadruple (top and bottom) bags: In vitro analysis of blood components!

BACKGROUND:As a part of continuous quality initiatives, while moving from triple bags to quadruple bags, we undertook a study to compare platelet-rich plasma (PRP) and buffy-coat (BC) methods with respect to all blood components (red blood cells [RBCs], random donor platelet concentrate [RDPC], and fresh frozen plasma [FFP]) prepared by PRP and BC methods.MATERIALS AND METHODS:It was a prospective analysis of different physical and quality parameters of RDPC, RBC, and FFP prepared out of 100 whole blood (WB) donations. Of these, 50 WB units were processed by PRP method using Triple bags and 50 WB units by BC method, using quadruple (top and bottom) bags, with an attached integral filter.RESULTS:RBC prepared by BC method had higher hematocrit (61.3 ± 1.91% vs. 56.03 ± 3.37%; P < 0.05) and lower white blood cell (WBC) contamination (6.3 × 104 ± 6.1 vs. 5.41 × 105 ± 2.5; P < 0.05) in comparison to prepared by PRP method. Higher PLT yield (7.67 × 1010 ± 1.8 vs. 6.47 × 1010 ± 1.5; P < 0.05) and lower WBC count (8.24 × 103 ± 1.1 vs. 1.5 × 104 ± 2.1; P < 0.05) was observed in RDPC prepared by BC method than PRP derived. CD62P expression was lower in RDPC prepared by BC method (31.46 ± 9.7%; P < 0.05) as compared to PRP method (43.35 ± 12.5%; P < 0.05). The BC method also resulted in increased plasma yield (210.56 ± 18.54 ml vs. 187.92 ± 12.93 ml; P < 0.05) in FFP in comparison to PRP method.CONCLUSION:The blood components produced from WB by the BC method have laboratory variables suggestive of superior quality than those produced by the PRP method.

Impact of blood alcohol concentration on hematologic and serum chemistry parameters in trauma patients: Analysis of data from a high-volume level 1 trauma center.

Background:Alcohol (EtOH) intoxication is common among trauma patients. While providers are familiar with the clinical aspects of acute EtOH intoxication, few studies have investigated the effects that EtOH levels may have on common laboratory markers. The aim of this study was to identify hematologic and serum chemistry parameters that may be affected by the blood alcohol concentration (BAC), hypothesizing that BAC influences both comprehensive blood count (CBC) and comprehensive serum chemistry (CSC) components.Methods:We performed an IRB-exempt institutional registry review of all trauma patients who had serum EtOH levels measured between January 2009 and June 2015. Data for each patient included: patient demographics, BAC determinations (g/dL), injury mechanism/severity information (ISS), hematologic parameters included in a CBC (hemoglobin, hematocrit, white blood cell [WBC] count, and platelet count), and CSC panel components (sodium, potassium, chloride, bicarbonate, blood urea nitrogen [BUN], creatinine, glucose, and hepatic function tests). Laboratory markers were contrasted across predefined categories of BAC: <0.10%, 10%–15%, 15%–20%, and >20%. Statistical comparisons were performed using SPSS 18 Software, employing analysis-of-covariance with adjustments performed for the patient demographics and injury characteristics. Statistical significance was set at α = 0.005.Results:A total of 2167 patient records were analyzed. After adjusting for patient age, gender, and ISS, increasing BAC correlated with 4.8% increase in hemoglobin and 32.5% higher hematocrit (both P < 0.001), as well as a 27.8% decrease in WBC count. There were also statistically significant differences between low (<0.10%) and high (>0.20%) BAC groups across multiple CSC parameters, with largest impact on BUN (32.2% decrease); creatinine (31.5% decrease); and glucose (13.6% decrease) values. Elevated BAC (>0.20 g/dL) was also associated with 81.8% increase in total bilirubin, and hepatic transaminases were elevated among patients with BAC >0.10.Conclusion:Due to the paucity of literature relating to the effects of BAC on serum hematologic and biochemical markers in acute trauma, this study provides a foundation for further exploration of these relationships and their clinical impact. More specifically, we found that BAC levels significantly influenced key laboratory markers, suggesting that acute EtOH intoxication may lead to hematologic and CSC changes that are potentially important in acute trauma management by frontline clinical staff.

Protective role of estrogen against excessive erythrocytosis in Monge\u2019s disease

Monge’s disease (chronic mountain sickness (CMS)) is a maladaptive condition caused by chronic (years) exposure to high-altitude hypoxia. One of the defining features of CMS is excessive erythrocytosis with extremely high hematocrit levels. In the Andean population, CMS prevalence is vastly different between males and females, being rare in females. Furthermore, there is a sharp increase in CMS incidence in females after menopause. In this study, we assessed the role of sex hormones (testosterone, progesterone, and estrogen) in CMS and non-CMS cells using a well-characterized in vitro erythroid platform. While we found that there was a mild (nonsignificant) increase in RBC production with testosterone, we observed that estrogen, in physiologic concentrations, reduced sharply CD235a+ cells (glycophorin A; a marker of RBC), from 56% in the untreated CMS cells to 10% in the treated CMS cells, in a stage-specific and dose-responsive manner. At the molecular level, we determined that estrogen has a direct effect on GATA1, remarkably decreasing the messenger RNA (mRNA) and protein levels of GATA1 (p < 0.01) and its target genes (Alas2, BclxL, and Epor, p < 0.001). These changes result in a significant increase in apoptosis of erythroid cells. We also demonstrate that estrogen regulates erythropoiesis in CMS patients through estrogen beta signaling and that its inhibition can diminish the effects of estrogen by significantly increasing HIF1, VEGF, and GATA1 mRNA levels. Taken altogether, our results indicate that estrogen has a major impact on the regulation of erythropoiesis, particularly under chronic hypoxic conditions, and has the potential to treat blood diseases, such as high altitude severe erythrocytosis.

Haematologic and Biochemical Parameters of Haemogregarine-infected ‎and Non-infected African Hinge-Back Tortoises in Ibadan, Nigeria

This study was conducted to evaluate haematological and biochemical parameters of haemogregarine-infected (h-infected) and non-infected African hinge-back tortoises in Ibadan, Nigeria. Blood samples were collected from 120 tortoises, of which 70 were Kinixys belliana and 50 were K. homeana. Stained thin smears were examined for haemogregarines using light microscope. Haematological and biochemical analyses were carried out following standard procedures. A total of 91(75.83 %) tortoises were positive for haemogregarines. Significantly (P&lt;0.05) lower values of haematocrit (23.92 %), haemoglobin (5.21g/dl) and mean corpuscular haemoglobin concentration (MCHC) (21.78 %) were recorded for h-infected tortoises with haematocrit (33.29 %), haemoglobin (8.31g/dl) and MCHC (24.96 %). Higher values of white blood cells (WBC) (7.26 x 109/L) and lymphocytes (2.71x109/L), were observed in h-infected than non-infected with WBC (5.58 x 109/L) and lymphocytes (2.15x109/L). Higher values of haematocrit and haemaglobin were recorded for K. Homeana. Males had higher haematocrit (27.27 %) and WBC (7.09 x 109/L) than females with haematocrit (24.35 %) and WBC (6.93 x 109/L). Females had higher MCHC, haemoglobin and calcium values than males.The lower values of haematocrit, haemoglobin and MCHC obtained for h-infected tortoises were expected since haemogregarines are usually found intra-erythrocytic in their host thereby destroying affected erythrocytes and causing a decrease in haematocrit value. Higher WBC counts in h-infected tortoises is typical in diseased conditions. The higher level of calcium in female tortoises is due to their reproductive cycle especially vitellogenesis and egg formation. Hypo-proteinaemia recorded in h- infected tortoises was attributed to parasitism. It is concluded that majority of haematological and biochemical analytes showed considerable variations with level of infection status, species and gender.

Hematocrit, age, and survival in a wild vertebrate population.

Understanding trade‐offs in wild populations is difficult, but important if we are to understand the evolution of life histories and the impact of ecological variables upon them. Markers that reflect physiological state and predict future survival would be of considerable benefit to unraveling such trade‐offs and could provide insight into individual variation in senescence. However, currently used markers often yield inconsistent results. One underutilized measure is hematocrit, the proportion of blood comprising erythrocytes, which relates to the blood's oxygen‐carrying capacity and viscosity, and to individual endurance. Hematocrit has been shown to decline with age in cross‐sectional studies (which may be confounded by selective appearance/disappearance). However, few studies have tested whether hematocrit declines within individuals or whether low hematocrit impacts survival in wild taxa. Using longitudinal data from the Seychelles warbler (Acrocephalus sechellensis), we demonstrated that hematocrit increases with age in young individuals (<1.5 years) but decreases with age in older individuals (1.5–13 years). In breeders, hematocrit was higher in males than females and varied relative to breeding stage. High hematocrit was associated with lower survival in young individuals, but not older individuals. Thus, while we did not find support for hematocrit as a marker of senescence, high hematocrit is indicative of poor condition in younger individuals. Possible explanations are that these individuals were experiencing dehydration and/or high endurance demands prior to capture, which warrants further investigation. Our study demonstrates that hematocrit can be an informative metric for life‐history studies investigating trade‐offs between survival, longevity, and reproduction.

A Retrospective Study in Dengue Positive Cases - Hematological and Biochemical Parameters (Liver Function Tests, Renal Function Tests) Correlation

Dengue fever is the most common arboviral infection in the world, and it is a disease with a broad clinical spectrum and a wide variety of presentations, ranging from asymptomatic to an undifferentiated fever (viral syndrome) to the more severe forms such as severe dengue (SD) or Dengue hemorrhagic fever (DHF). The diagnosis of dengue fever is carried out based on clinical, epidemiological and laboratory data. Among laboratory tests, both non-specific [blood count, platelet count, tourniquet test, prothrombin time (PT), activated partial thromboplastin time (APTT), liver function tests and serum albumin concentration] and specific tests (viral isolation tests and serology for antibody examination) are used. The principle aim of our study is to assess and compare the biochemical and haematological parameters of patients with dengue. In our study population, the peak frequency of the patients who were dengue positive was on the age group between 21 and 30 years (44%), and it was more common among males. The most common serological indicator of dengue fever was the presence of NS1 antibody. High haematocrit (56%) was found in most of the patients, and the next common finding was thrombocytopenia (51%). Elevated Serum glutamic oxaloacetic transaminase seen in 53% of cases and Elevated Serum glutamic pyruvic transaminase, Alkaline Phosphatase, Serum Bilirubin, Serum creatinine and Serum urea were found in 41%, 11%,7%, 4%, 3%of cases respectively. The knowledge about these parameters helps the clinician in early diagnosis of the cases and initiation of the management measures before the drastic clinical features of the disease sets.

Haematological indices of grower pigs fed low protein and low energy diets supplemented with multi-enzyme

One of the reliable information for evaluating nutritional status, performance and physiological state of farm animals to nutrient utilization is through haematological indices assessment. Haematological parameters of grower pigs fed low crude protein and low energy diets supplemented with multi-enzyme (enziblend plus+) were determined using 36 hybrid (Landrace x Large white) male pigs of 8-10 weeks old. Two metabolizable energy (3000 and 2600 Kcal ME/kg) and three crude protein levels (18, 16 and 14 % CP) were used to formulate Six dietary treatments; T1 (3000 Kcal ME/Kg; 18 % CP), T2 (3000 Kcal ME/Kg; 16 % CP+ 1g of enzyme/kg diet), T3 (3000 Kcal ME/Kg; 14 % CP+ 1g of enzyme/kg diet), T4 (2600 Kcal ME/Kg; 18 % CP + 1g of enzyme/kg diet), T5 (2600 Kcal ME/Kg; 16 % CP + 1g of enzyme/kg diet) and T6 (2600 Kcal ME/Kg; 14 % CP + 1g of enzyme/kg diet). The treatments were replicated three with two pigs per replicate. The experiment was a 2 x 3 factorial, and T1 served as the control. Blood samples were collected (3mls) via the ear vein from one pig in each replicate, three from treatment for haematological analysis. The results of this study recorded significant differences (P&lt;0.05) in most of the hematological parameters evaluated, except in mean corpuscular haemoglobin concentration (MCHC) and basophile counts of the pigs. Grower pigs fed T2 and T4 diets recorded significantly (P&lt;0.05) higher packed cell volume (PCV) (40.15% and 40.22%), hemoglobin (12.25g/dl and 12.00g/dl), red blood cell (7.95 x106 / µl and 8.00 x106 / µl), white blood cell (8.12 x103 / µl and 7.91 x103 / µl) and lymphocyte counts (5.26 x103 / µl and 5.00 x103 / µl). Grower pigs on T6 recorded significantly (P &lt;0.05) lower mean values for PCV (32.55%), hemoglobin concentration (Hb) (9.82g/dl), red blood cell (RBC) (5.32 x103 / µl), white blood cell (WBC) (6.10 x103 / µl), lymphocyte (3.10 x103 / µl) and monocyte (0.31 x103 / µl) counts, respectively. Based on the findings of this study, feeding of growing pigs with low energy and low crude protein diets below 16 % CP with multi-enzyme supplementation adversely effected PCV, hemoglobin, RBC, WBC, lymphocytes and monocytes of grower pigs and therefore should be avoided in pig production.

To Retrograde Autologous Prime or Not?

To Retrograde Autologous Prime or Not?

Correlation between Anemia and Malaria Infection Severity in Patients with Type 2 Diabetes Mellitus in Nigeria

Background: Malaria and diabetes mellitus are still a significant global public health problem despite the phenomenal progresses in clinical sciences related to the diseases. Both anemia and malaria parasitemia are common in developing countries. It is therefore important to diagnose and determine the correlation between anemia level and malaria infection severity in diabetic patients for better management. Methods: Patients with diabetes mellitus (DM) infected with malaria were recruited (n=50) as subjects and non-diabetic patients were used as control in this study, who were further divided into two sub-group: non-DM infected with malaria (n=25) and non-DM without malaria parasitemia (n=25). Blood sample were collected to examine the fasting blood sugar (FBS) level, packed cell volume (PCV), hemoglobin (Hb) level, and malaria parasitemia. Statistical analysis was then performed using ANOVA with a p value of less than or equal to 0.05 considered statistically significant. Results: The parasite density in DM with malaria was significantly lower (p&lt;0.05) than in the non-DM with malaria. Interestingly, there was a higher PCV and hemoglobin level (p&lt;0.05) in DM with malaria when compared with non-DM with malaria. Conclusion: DM patients infected with plasmodium have low parasite density but higher hemoglobin level and PCV compared to the control group. There is no correlation between the severity of anemia and malaria parasitemia in DM patients infected with malaria when compared to non-diabetic subjects infected with malaria. Further studies are needed to explore the correlation between hemoglobin level in DM and plasmodium infection

Correlation of ultrasound measurement of IVC to aorta diameter ratio with hematocrit and shock severity among children with dengue fever

Background: Severe dengue is a leading cause of serious illness and death, particularly in the Philippines. There is no specific treatment for dengue fever. Before fluid resuscitation, a baseline hematocrit is obtained, and frequent monitoring of the complete blood count is needed depending on the case of the patient. Aside from obtaining the hematocrit, the IVC/Ao diameter ratio can be a promising tool in objectively guiding the clinicians during the fluid resuscitation in real-time. This study aims to determine the correlation of ultrasound measurement of IVC to aorta diameter ratio with hematocrit and severity of shock among children with dengue fever seen at the emergency room. Methods and materials: This is a prospective observational study design that was conducted at the ER of a tertiary hospital. Clinical data and hematocrit of the children with dengue fever aged 1 m to 18 y old were documented. The Inferior vena cava diameter (IVC)and the Aorta (Ao) diameter ratio was determined using bedside ultrasound. Results: The IVC/Ao diameter ratio correlates with high hematocrit and dengue shock in children (p < 0.0001). There is a significantly higher proportion of subjects with a hematocrit of normal range of age among those with a ratio 1.0. A ratio of <0.8 is significantly associated with high hematocrit level for age (p < 0.0001). Overall, IVC/Ao diameter ratio was significantly associated with severity of Dengue illness. 97% of those without warning signs had IVC/Ao diameter ratio of 1.00 (p < 0.0001). For those with warning signs, 63.4% had a ratio of from 0.8–0.99, and 93% with severe dengue had a ratio of from <0.80 (p < 0.0001). Sensitivity or the probability of identifying those with true disease, of IVC/Ao diameter ratio of <1.00 in predicting dengue with warning signs and severe dengue (grouped) was 87.7%. Specificity or the probability of identifying those without the disease (without warning signs) was higher at 97.1%. Measurement of IVC/Ao diameter ratio has high interrater and intrarater reliability. Conclusion: This study concludes that the IVC/Ao ratio correlates with the severity of dengue fever and with the hematocrit in the emergency setting. Overall, IVC/Ao diameter ratio was significantly associated with severity of dengue illness.

PSV-1 A Subcutaneous Ear Implant to Deliver an Anaplasmosis Vaccine to Dairy Steers

Abstract Bovine anaplasmosis is the most prevalent tick-transmitted disease of cattle worldwide and a major obstacle to beef production. The objective of the study was to test the ability of a single-dose delivery platform to produce long-lasting immunity against anaplasmosis infections. Twelve Holstein steers were administered a 3-stage, single-dose vaccine against Anaplasma marginale major surface protein 1a. The vaccine consisted of a soluble vaccine administered subcutaneously for immune priming, a biodegradable polyanhydride depot for intermediate slow-release of the vaccine for boosting the immune response, and an immune-isolated vaccine platform for extended antigen release deposited subcutaneously in the ear. Six calves were randomly assigned to two vaccine constructs that featured rods and implants containing a combination of two adjuvants (DEAE-Dextran and Quil-A). The remaining 6 calves were randomly assigned to two vaccine constructs that featured rods and implants containing the same adjuvant (DEAE-Dextran or Quil-A). Twenty months post-implantation, calves were challenged with Anaplasma marginale stabilate and were monitored weekly for signs of fever, decreased packed cell volume (PCV) and bacteremia. Outcome variables (fever, PCV, bacteremia) were analyzed using a linear mixed model (PROC GLMMIX; SAS University Edition v9.04.01, SAS Institute, Cary, NC). Two-tailed chi-squared tests were used to compare need for antibiotic intervention between vaccine constructs. Calves within the combination adjuvant construct group (DEAE-D and Quil-A) exhibited significantly (P = .006) higher PCV than calves within the single adjuvant construct group (DEAE-Dextran or Quil-A). Likewise, calves within the combination adjuvant group required significantly (P = .014) fewer antibiotic interventions compared with calves in the single adjuvant group. Findings of this study suggest that calves exhibited significantly diminished clinical signs of anaplasmosis when vaccine was delivered with a combination of adjuvants as opposed to a single adjuvant in a single-dose delivery platform.